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Gene Dmel\Rbp1

General Information
Symbol	Dmel\Rbp1	Species	D. melanogaster
Name	RNA-binding protein 1	Annotation symbol	CG17136
Feature type	protein_coding_gene	FlyBase ID	FBgn0260944
Gene Model Status	Current	Stock availability	3719 publicly available
Also Known As	RRM1, Rbp-1, RRM11
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	86C6-86C6	Sequence location	3R:6,610,104..6,612,187 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene RNA-binding protein 1 is referred to in FlyBase by the symbol Dmel\Rbp1 (CG17136, FBgn0260944). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: nuclear mRNA cis splicing, via spliceosome; RNA splicing. 30 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult spermathecae, adult male reproductive system, larval/adult carcass. Gene sequence location is 3R:6610104..6612187.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms transcriptionally active chromatin nuclear mRNA cis splicing, via spliceosome
FB2012_01	Sequence features BKN41855 HFA16817
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 86C6-86C6   Limits computationally determined from genome sequence between P{PZ}hth05745 and P{EP}EP3340EP3340
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rbp1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rbp1-RA FBtr0082275  NM_079583   774   135 Rbp1-RD FBtr0082277  NM_169364   1513   144
Additional Transcript Data & Comments
Reported size (kB)	5, 2.7, 2.0, 0.8 (northern blot) (Kim et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rbp1-PA   FBpp0081752   15.4   135   11.83     NP_524307   AAF54555 Rbp1-PD   FBpp0081754   16.0   144   10.34     NP_731510   AAN13487
Additional Polypeptide Data & Comments
Reported size (kDa)	135 (aa); 27 (kD observed); 15 (kD predicted) (Kim et al., 1992)
Comments	Indirect immunofluorescence reveals that Rbp1 protein is localized at a limited number of sites along the larval salivary gland polytene chromosomes. Furthermore, it was shown that the Rbp1 protein localization pattern is the same as the RNA polymerase II localization pattern on the polytene chromosomes. Splicing assays in human cell extracts show that Rbp1 is capable of activating splicing and switching splice site selection. (Kim et al., 1992)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695323   AE014297 AAF54555   AE014297 AAN13487   AI297394   AI387802   AI388366   AY069252 AAL39397   BI164560   BI165732   BI165818   BI172346   BI358745   BI485636   BI564386   BI564778   BI565027   BI568745   BI568773   BI573915   BI573916   BI575604   BI581128   BI581623   BI582750   BI585037   BI586775   BI607731   BI620095   BI624130   BI628050   BI632071   BI634143   BP543283   BP543284   BP546560   BP548911   BP548912   BT120323   CK086549   CO187065   CO264866   CO273985   CO274502   CO280521   CO281108   CO293046   CO294544   CO296054   CO298927   CO302316   CO303256   CO303652   CO305270   CO306551   CO306731   CO318316   CO326908   CO332058   CO337921   CO339340   CO340120   CO340412   CO343811   CZ479104   CZ480726   EC061852   EC066910   EC078739   EC086763   EC088761   EC089671   EC194994   EC198107   EC209728   EC209815   EC212688   EC212935   EC214097   EC214418   EC214605   EC232866   EC235658   EC248943   EC251072   EC253154   EC254300   EC258129   EV588857   EV591416   L04929 AAA28850   S51691 AAB24622   S51740 AAB24631
UniProtKB/Swiss-Prot	Q02427
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4   organism   • yolk plasma | posterior (Lecuyer et al., 2007) embryonic stage 5   pole cell   (Lecuyer et al., 2007) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Kim et al., 1992) third instar larval stage     (Kim et al., 1992) pupal stage     (Kim et al., 1992) adult stage     (Kim et al., 1992)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term	nucleus polytene chromosome (Kim et al., 1992)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0260944    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0260944 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0260944 expression level Developmental Stage   Expression Level embryo 00-02hr    3266 embryo 02-04hr    4591 embryo 04-06hr    5871 embryo 06-08hr    5983 embryo 08-10hr    5318 embryo 10-12hr    4939 embryo 12-14hr    2523 embryo 14-16hr    2075 embryo 16-18hr    1542 embryo 18-20hr    1373 embryo 20-22hr    1383 embryo 22-24hr    1278 larva L1    1830 larva L2    2560 larva L3 12hr old    1835 larva L3 puffstage 1-2    1437 larva L3 puffstage 3-6    1833 larva L3 puffstage 7-9    2250 white prepupae new    2125 white prepupae 12hr    2151 white prepupae 24hr    2967 pupae 2d postWPP    1928 pupae 3d postWPP    1070 pupae 4d postWPP    965 adult male 01day    1163 adult male 05day    1522 adult male 30day    1354 adult female 01day    1724 adult female 05day    2438 adult female 30day    2579 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3266) embryo 02-04hr  (4591) embryo 04-06hr  (5871) embryo 06-08hr  (5983) embryo 08-10hr  (5318) embryo 10-12hr  (4939) embryo 12-14hr  (2523) embryo 14-16hr  (2075) embryo 16-18hr  (1542) embryo 18-20hr  (1373) embryo 20-22hr  (1383) embryo 22-24hr  (1278) larva L1  (1830) larva L2  (2560) larva L3 12hr old  (1835) larva L3 puffstage 1-2  (1437) larva L3 puffstage 3-6  (1833) larva L3 puffstage 7-9  (2250) white prepupae new  (2125) white prepupae 12hr  (2151) white prepupae 24hr  (2967) pupae 2d postWPP  (1928) pupae 3d postWPP  (1070) pupae 4d postWPP  (965) adult male 01day  (1163) adult male 05day  (1522) adult male 30day  (1354) adult female 01day  (1724) adult female 05day  (2438) adult female 30day  (2579) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3266) embryo 02-04hr  (4591) embryo 04-06hr  (5871) embryo 06-08hr  (5983) embryo 08-10hr  (5318) embryo 10-12hr  (4939) embryo 12-14hr  (2523) embryo 14-16hr  (2075) embryo 16-18hr  (1542) embryo 18-20hr    1373 embryo 20-22hr    1383 embryo 22-24hr    1278 larva L1  (1830) larva L2  (2560) larva L3 12hr old  (1835) larva L3 puffstage 1-2  1437 larva L3 puffstage 3-6  (1833) larva L3 puffstage 7-9  (2250) white prepupae new  (2125) white prepupae 12hr  (2151) white prepupae 24hr  (2967) pupae 2d postWPP  (1928) pupae 3d postWPP    1070 pupae 4d postWPP    965 adult male 01day    1163 adult male 05day  (1522) adult male 30day    1354 adult female 01day  (1724) adult female 05day  (2438) adult female 30day  (2579) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3266 embryo 02-04hr    4591 embryo 04-06hr    5871 embryo 06-08hr    5983 embryo 08-10hr    5318 embryo 10-12hr    4939 embryo 12-14hr    2523 embryo 14-16hr    2075 embryo 16-18hr    1542 embryo 18-20hr    1373 embryo 20-22hr    1383 embryo 22-24hr    1278 larva L1    1830 larva L2    2560 larva L3 12hr old    1835 larva L3 puffstage 1-2    1437 larva L3 puffstage 3-6    1833 larva L3 puffstage 7-9    2250 white prepupae new    2125 white prepupae 12hr    2151 white prepupae 24hr    2967 pupae 2d postWPP    1928 pupae 3d postWPP    1070 pupae 4d postWPP    965 adult male 01day    1163 adult male 05day    1522 adult male 30day    1354 adult female 01day    1724 adult female 05day    2438 adult female 30day    2579 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3266 embryo 02-04hr    4591 embryo 04-06hr    5871 embryo 06-08hr    5983 embryo 08-10hr    5318 embryo 10-12hr    4939 embryo 12-14hr    2523 embryo 14-16hr    2075 embryo 16-18hr    1542 embryo 18-20hr    1373 embryo 20-22hr    1383 embryo 22-24hr    1278 larva L1    1830 larva L2    2560 larva L3 12hr old    1835 larva L3 puffstage 1-2    1437 larva L3 puffstage 3-6    1833 larva L3 puffstage 7-9    2250 white prepupae new    2125 white prepupae 12hr    2151 white prepupae 24hr    2967 pupae 2d postWPP    1928 pupae 3d postWPP    1070 pupae 4d postWPP    965 adult male 01day    1163 adult male 05day    1522 adult male 30day    1354 adult female 01day    1724 adult female 05day    2438 adult female 30day    2579 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0260944 expression level Developmental Stage   Expression Level embryo 00-02hr    3266 embryo 02-04hr    4591 embryo 04-06hr    5871 embryo 06-08hr    5983 embryo 08-10hr    5318 embryo 10-12hr    4939 embryo 12-14hr    2523 embryo 14-16hr    2075 embryo 16-18hr    1542 embryo 18-20hr    1373 embryo 20-22hr    1383 embryo 22-24hr    1278 larva L1    1830 larva L2    2560 larva L3 12hr old    1835 larva L3 puffstage 1-2    1437 larva L3 puffstage 3-6    1833 larva L3 puffstage 7-9    2250 white prepupae new    2125 white prepupae 12hr    2151 white prepupae 24hr    2967 pupae 2d postWPP    1928 pupae 3d postWPP    1070 pupae 4d postWPP    965 adult male 01day    1163 adult male 05day    1522 adult male 30day    1354 adult female 01day    1724 adult female 05day    2438 adult female 30day    2579 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3266) embryo 02-04hr  (4591) embryo 04-06hr  (5871) embryo 06-08hr  (5983) embryo 08-10hr  (5318) embryo 10-12hr  (4939) embryo 12-14hr  (2523) embryo 14-16hr  (2075) embryo 16-18hr  (1542) embryo 18-20hr  (1373) embryo 20-22hr  (1383) embryo 22-24hr  (1278) larva L1  (1830) larva L2  (2560) larva L3 12hr old  (1835) larva L3 puffstage 1-2  (1437) larva L3 puffstage 3-6  (1833) larva L3 puffstage 7-9  (2250) white prepupae new  (2125) white prepupae 12hr  (2151) white prepupae 24hr  (2967) pupae 2d postWPP  (1928) pupae 3d postWPP  (1070) pupae 4d postWPP  (965) adult male 01day  (1163) adult male 05day  (1522) adult male 30day  (1354) adult female 01day  (1724) adult female 05day  (2438) adult female 30day  (2579) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3266) embryo 02-04hr  (4591) embryo 04-06hr  (5871) embryo 06-08hr  (5983) embryo 08-10hr  (5318) embryo 10-12hr  (4939) embryo 12-14hr  (2523) embryo 14-16hr  2075 embryo 16-18hr  1542 embryo 18-20hr    1373 embryo 20-22hr    1383 embryo 22-24hr    1278 larva L1  1830 larva L2  (2560) larva L3 12hr old  1835 larva L3 puffstage 1-2  1437 larva L3 puffstage 3-6  1833 larva L3 puffstage 7-9  2250 white prepupae new  2125 white prepupae 12hr  2151 white prepupae 24hr  (2967) pupae 2d postWPP  1928 pupae 3d postWPP    1070 pupae 4d postWPP    965 adult male 01day    1163 adult male 05day  1522 adult male 30day    1354 adult female 01day  1724 adult female 05day  2438 adult female 30day  (2579) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3266 embryo 02-04hr    4591 embryo 04-06hr    5871 embryo 06-08hr    5983 embryo 08-10hr    5318 embryo 10-12hr    4939 embryo 12-14hr    2523 embryo 14-16hr    2075 embryo 16-18hr    1542 embryo 18-20hr    1373 embryo 20-22hr    1383 embryo 22-24hr    1278 larva L1    1830 larva L2    2560 larva L3 12hr old    1835 larva L3 puffstage 1-2    1437 larva L3 puffstage 3-6    1833 larva L3 puffstage 7-9    2250 white prepupae new    2125 white prepupae 12hr    2151 white prepupae 24hr    2967 pupae 2d postWPP    1928 pupae 3d postWPP    1070 pupae 4d postWPP    965 adult male 01day    1163 adult male 05day    1522 adult male 30day    1354 adult female 01day    1724 adult female 05day    2438 adult female 30day    2579 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3266 embryo 02-04hr    4591 embryo 04-06hr    5871 embryo 06-08hr    5983 embryo 08-10hr    5318 embryo 10-12hr    4939 embryo 12-14hr    2523 embryo 14-16hr    2075 embryo 16-18hr    1542 embryo 18-20hr    1373 embryo 20-22hr    1383 embryo 22-24hr    1278 larva L1    1830 larva L2    2560 larva L3 12hr old    1835 larva L3 puffstage 1-2    1437 larva L3 puffstage 3-6    1833 larva L3 puffstage 7-9    2250 white prepupae new    2125 white prepupae 12hr    2151 white prepupae 24hr    2967 pupae 2d postWPP    1928 pupae 3d postWPP    1070 pupae 4d postWPP    965 adult male 01day    1163 adult male 05day    1522 adult male 30day    1354 adult female 01day    1724 adult female 05day    2438 adult female 30day    2579 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0260944    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0260944 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult spermathecae, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260944
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Rbp1e04474 viable, with Scer\GAL4pnr-MD237 Rbp1GD9289 Sterility Allele fertile Rbp1e04474 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Rbp1 Class Mutagen Stocks Known lesion Rbp1e04474 piggyBac transposase 2 -- Rbp1e01298 piggyBac transposase 1 -- Rbp1e02960 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 27 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rbp1 Class Mutagen Stocks Known lesion Rbp1SA.RB in vitro construct 3718 Yes Rbp1GD9289 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rbp1Act5C.PH in vitro construct - regulatory fusion in vitro construct 0 Yes Rbp1ALT.MtnA in vitro construct - regulatory fusion 0 Yes Rbp1delRS.MtnA in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Rbp1GlyG-A.Act5C in vitro construct - deletion 0 Yes Rbp1GlyR-K.Act5C in vitro construct - deletion 0 Yes Rbp1GlyS-T.Act5C in vitro construct - deletion 0 Yes Rbp1KK115799 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Rbp1RS.MtnA in vitro construct - regulatory fusion 0 Yes Rbp1Sub109,111-115.Act5C in vitro construct - deletion 0 Yes Rbp1SubRNP.Act5C in vitro construct - deletion 0 Yes Rbp1T:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes Rbp1Δ107-114.Act5C in vitro construct - deletion 0 Yes Rbp1Δ115-124.Act5C in vitro construct - deletion 0 Yes Rbp1Δ115-135.Act5C in vitro construct - deletion 0 Yes Rbp1Δ125-135.Act5C in vitro construct - deletion 0 Yes Rbp1ΔPvu.Act5C in vitro construct - deletion 0 Yes Rbp1ΔRRM-Gly.Act5C in vitro construct - deletion 0 Yes Rbp1ΔRRM.T:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes Rbp1ΔRS.T:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes Rbp1ΔSR+1.Act5C in vitro construct - deletion 0 Yes Rbp1ΔSR+1R-K.Act5C in vitro construct - deletion 0 Yes Rbp1ΔSR+2.Act5C in vitro construct - deletion 0 Yes Rbp1ΔSR+3.Act5C in vitro construct - deletion 0 Yes Rbp1ΔSR+3SRSR.Act5C in vitro construct - deletion 0 Yes Rbp1ΔSR.Act5C in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)cu (Kumar and Lopez, 2005) Df(3R)Exel6159 (Ryder, 2004.4.26) Df(3R)Exel7305 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9289} NA P{KK115799} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}Rbp1e01298 NA PBac{RB}Rbp1e02960 NA PBac{RB}Rbp1e04474 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Trn-SR (Allemand et al., 2002)
Biological Process
CV term Evidence References
nuclear mRNA cis splicing, via spliceosome inferred from direct assay (Kim et al., 1992) RNA splicing inferred from mutant phenotype (Kumar and Lopez, 2005)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Allemand et al., 2002, Kim et al., 1992) transcriptionally active chromatin inferred from direct assay (Kim et al., 1992)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) non-traceable author statement (Kim, 1993.2.3, Kim, 1993.3.10, Kim, 1993.3.10) nucleic acid binding inferred from electronic annotation with InterPro:IPR000504 (FlyBase Curators et al., 2004-) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
mRNA splice site selection inferred from sequence or structural similarity with NCBI:NP_003008 (Mount and Salz, 2000) nuclear mRNA splicing, via spliceosome inferred from sequence or structural similarity with NCBI:NP_003008 (Mount and Salz, 2000) non-traceable author statement (Kim, 1993.2.3, Duttaroy and Mount, 1998)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 1998.11.1)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rbp1 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66438 DroID - A comprehensive database of gene and protein interactions. • FBgn0260944 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41294
Orthologs
Genome-wide drosophilid orthologs	Dana\GF17902 Dere\GG17683 Dmoj\GI23736 Dsec\GM23896 Dsim\GD18708 Dvir\GJ10582 Dwil\GK13897 Dyak\GE26048
Curated drosophilid orthologs
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • 152479 Branchiostoma floridae (lancelet) BGIBMGA007656-PA Bombyx mori (silk worm) CPIJ003074 Culex pipens (mosquito) ENSGACP00000001909 Gymnopilus aculeatus (mushroom) ISCW021699-PA Ixodes scapularis (deer tick) ENSOANP00000024681 Ornithorynchus anatinus (platypus) 95292 Phytophthora ramorum (sudden oak death pathogen) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB11874 LH18882 FBgn0243090 FBgn0233705 FBgn0218403 FBgn0215904 FBgn0201978 FBgn0190229 FBgn0178761 JGI_V11_304081 FBgn0075198 FBgn0164306 FBgn0138086 FBgn0030479 FBgn0131928 FBgn0111709 FBgn0109905 FBgn0096507 FBgn0094920 CPIJ003074 BGIBMGA007656 GB13703 AGAP007325 AAEL001356 PHUM357390 GLEAN_04361
Stocks & Reagents
Stocks Listed in FlyBase ( 3719 )
Harvard	- PBac{RB}e00002 - PBac{RB}e00005 - PBac{RB}e00013 - PBac{RB}e00015 - PBac{RB}e00018 - PBac{RB}e00022 - PBac{RB}e00023 - PBac{RB}e00025 - PBac{RB}e00030 - PBac{RB}e00031 - PBac{RB}e00035 - PBac{RB}e00041
	More stocks available...
Genomic Clones ( 3 )
	BACR15J23 BACR29B06 BACR32N16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 81 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM02602 RE57382
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH18462 GH19248 GM31486 LP11684 RE04260 RE05677 RE05777 RE14693 RE64713 RE72367 RH02934 RH19149 RH21049 RH24478 RH26874 RH31340 RH33973 RH35242 RH36919 RH37363 RH48315 RH54948 RH60119 RH62283 RH62661 RH64746 RH71514
Other clones	12154 29221 29397 30760 40113 72888 74592 75147 78751 108287 120525 121109 125562 144986 166188 173756 182083 214219.33 214511.47 214513.37 274589 CK01.29B.H8 Dmel_neb_010880_0072_3574 Dmel_neb_012824_0392_1053 EK025964 EK112828 EK114308 EK140534 EK154252 EK156004 EK174131 EK183903 EK201557 EK207153 EK212603 EK240703 EK242320 EK247850 EK251417 EK252059 EK271529 EK302414 EN15604 EP01639 EP04943 EP10046 EP10604 EP18108 FGC019F06 FGH107F12 FGM002E10 RH09376
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0260944 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41294
Antibody Information
	polyclonal (Kim et al., 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Rbp1 Rbp11 (FlyBase, 1996-)
Additional comments
Other Comments
	dsRNA made from templates generated with primers directed against this gene. (Kumar and Lopez, 2005) Pathway interactions indicate a pivotal role of Rbp1 connecting splicing machinery to the upstream components of the sex-determination pathway. (Giot et al., 2003) Protein-protein interaction studies between Rbp1 and itself and between Rbp1 and tra2 suggest that Rbp1 and tra2 function in regulation of dsx splicing by forming a complex. (Heinrichs and Baker, 1997) Both HeLa and Kc cell nuclear extracts have been used for UV cross-linking experiments to determine which proteins bind to dsxRE as part of the native tra- and tra2-dependent dsx enhancer complex (dsxEC). Rbp1 and SRp30 have been identified that bind the 13-nucleotide repeats and purine rich element (PRE), respectively, of the dsx repeat element (dsxRE). (Lynch and Maniatis, 1996) The RNA target sequences recognised by Rbp1 have been determined using the in vitro selection approach and were found within the repeat region and in the purine rich region polypyrimidine tract of the regulated female specific 3' splice site of dsx. The Rbp1 protein can activate female specific splicing of dsx in vivo by recognising target sequences present within the pre-mRNA. (Heinrichs and Baker, 1995) A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs. (Kim and Baker, 1993) The general nuclear expression pattern, colocalization on chromosomes with RNA polymerase II, the similarity to human splicing factor and the effect on alternative splicing in vitro all suggest that Rbp1 is involved in the processing of precursor mRNAs. (Kim et al., 1992)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079583 NM_169364 RefSeq (Proteins) • NP_524307 NP_731510 DDBJ / EMBL / GenBank • AA695323 AAA28850 AAB24622 AAB24631 AAF54555 AAL39397 AAN13486 AAN13487 AAN13488 AY069252 CZ479104 CZ480726 L04929 S51691 S51740 Entrez Gene - A searchable database of RefSeq genes. • 41294 UniProtKB/Swiss-Prot • Q02427
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677)
Linkouts
	BioGRID - A database of protein and genetic interactions • 66438 DroID - A comprehensive database of gene and protein interactions. • FBgn0260944 DRSC - Results from RNAi screens. • FBgn0260944 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260944 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0260944 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41294 InParanoid A subset of ortholog calls from InParanoid. • 152479 Branchiostoma floridae (lancelet) BGIBMGA007656-PA Bombyx mori (silk worm) CPIJ003074 Culex pipens (mosquito) ENSGACP00000001909 Gymnopilus aculeatus (mushroom) ISCW021699-PA Ixodes scapularis (deer tick) ENSOANP00000024681 Ornithorynchus anatinus (platypus) 95292 Phytophthora ramorum (sudden oak death pathogen) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41294 modMine - Data generated by the modENCODE project. • FBgn0260944 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB11874 LH18882 FBgn0243090 FBgn0233705 FBgn0218403 FBgn0215904 FBgn0201978 FBgn0190229 FBgn0178761 JGI_V11_304081 FBgn0075198 FBgn0164306 FBgn0138086 FBgn0030479 FBgn0131928 FBgn0111709 FBgn0109905 FBgn0096507 FBgn0094920 CPIJ003074 BGIBMGA007656 GB13703 AGAP007325 AAEL001356 PHUM357390 GLEAN_04361
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	CG17136 (Mount and Salz, 2000, Keleman et al., 2009.8.5) RBP1 (Smith and Valcarcel, 2000, Kumar and Lopez, 2004, Hoffman and Lis, 2000, Tacke and Manley, 1999, Mount et al., 1999, Graveley et al., 1999, Du et al., 1998, Heinrichs and Baker, 1997, Lynch and Maniatis, 1996, Manley and Tacke, 1996, Kpebe and Rabinow, 2007) rbp1 (Swiss-Prot Project Members, 1998.11.1, Kim, 1993.2.3, Heinrichs et al., 1998, Wan et al., 2008) Rbp-1 (Burnette et al., 1999, Lecuyer et al., 2007) Rbp1 (Burnette, 2005, Qi et al., 2007, Chandler et al., 2003, Labourier and Rio, 2001) Rbp11   RRM1 (Kim, 1993.3.10, ) rrm1 (Kim and Baker, 1993) RRM11 (Kim, 1993.3.10, ) rrm11 (Kim and Baker, 1993)
Name Synonym	RNA-binding protein 1   RNA-binding protein 11
Secondary FlyBase IDs
	FBgn0010252 FBgn0010254
References ( 60 )
Generate a list of	All references relating to this gene ( 60 )
List References by type	Research paper  ( 24 ) Supplementary material  ( 3 ) Review  ( 6 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 9 ) FlyBase analysis  ( 2 ) Protein sequence record  ( 1 ) DNA/RNA sequence record  ( 3 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...