RRM1, Rbp-1, RRM11
Gene model reviewed during 5.49
None of the polypeptides share 100% sequence identity.
135 (aa); 27 (kD observed); 15 (kD predicted)
Indirect immunofluorescence reveals that Rbp1 protein is localized at a limited number of sites along the larval salivary gland polytene chromosomes. Furthermore, it was shown that the Rbp1 protein localization pattern is the same as the RNA polymerase II localization pattern on the polytene chromosomes. Splicing assays in human cell extracts show that Rbp1 is capable of activating splicing and switching splice site selection.
Extensively phosphorylated on serine residues in the RS domain.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rbp1 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Rbp1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: Rbp1 Rbp11
dsRNA made from templates generated with primers directed against this gene.
Pathway interactions indicate a pivotal role of Rbp1 connecting splicing machinery to the upstream components of the sex-determination pathway.
Both HeLa and Kc cell nuclear extracts have been used for UV cross-linking experiments to determine which proteins bind to dsxRE as part of the native tra- and tra2-dependent dsx enhancer complex (dsxEC). Rbp1 and SRp30 have been identified that bind the 13-nucleotide repeats and purine rich element (PRE), respectively, of the dsx repeat element (dsxRE).
The RNA target sequences recognised by Rbp1 have been determined using the in vitro selection approach and were found within the repeat region and in the purine rich region polypyrimidine tract of the regulated female specific 3' splice site of dsx. The Rbp1 protein can activate female specific splicing of dsx in vivo by recognising target sequences present within the pre-mRNA.
A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs.
The general nuclear expression pattern, colocalization on chromosomes with RNA polymerase II, the similarity to human splicing factor and the effect on alternative splicing in vitro all suggest that Rbp1 is involved in the processing of precursor mRNAs.