FlyBase Gene Report: Dmel\CG42594

General Information

Symbol

Dmel\CG42594

Species

D. melanogaster

Name

Annotation Symbol

CG42594

Feature Type

protein_coding_gene

FlyBase ID

FBgn0260971

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

4D2-4D5

Sequence location

X:4,840,833..4,906,575 [+]

Recombination map

1-9

RefSeq locus

NC_004354 REGION:4840833..4906575

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables outward rectifier potassium channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000108561

(GO Reference Genome Project, 2011-)

enables potassium channel activity

inferred from electronic annotation with InterPro:IPR003280

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:Task7; FB:FBgn0037690,FLYBASE:Task6; FB:FBgn0038165

(Gene Ontology Curators, 2002-)

enables potassium ion leak channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000108561

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in potassium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000108561

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR003280

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:Task7; FB:FBgn0037690,FLYBASE:Task6; FB:FBgn0038165

(Gene Ontology Curators, 2002-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from sequence or structural similarity with FLYBASE:Task7; FB:FBgn0037690,FLYBASE:Task6; FB:FBgn0038165

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR003280

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000108561

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

TWO-PORE DOMAIN K⁺ CHANNEL SUBUNITS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

TWO-PORE DOMAIN K⁺ CHANNEL SUBUNITS -: The two-pore domain K⁺ (K2P) channels possess four transmembrane domains and two pore domains; they assemble as dimers. K2P channels have been implicated in a number of processes such as maintaining resting potential (leak K⁺ conductance), responding to volatile anesthetics, chemo- and mechanosensation. (Adapted from FBrf0188365 and PMID:11121510).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG42594 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry E1JJG7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.56

Gene model reviewed during 5.52

Gene model reviewed during 5.39

Gene model reviewed during 5.55

Gene model reviewed during 5.57

Stop-codon suppression (UGA) postulated; (FBrf0223588, FlyBase analysis); supported by conservation of the extended ORF across Drosophila species (FBrf0202906).

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG42594-RA

FBtr0301749

NM_001169196

4812

905

CG42594-RC

FBtr0345703

NM_001169197

4812

1039

CG42594-RD

FBtr0345704

NM_001297940

5127

905

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG42594-PA

FBpp0290963

95.2

905

7.85

FBpp0311747

109.4

1039

6.89

FBpp0311748

95.2

905

7.85

E1JJG8

NP_001284869

AHN59340

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

905 aa isoforms: CG42594-PA, CG42594-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG42594 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG42594_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.58

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG42594 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG42594

View exonic expression by tissue for Dmel\CG42594

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

8674091/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260971

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260971

Flygut - An atlas of the Drosophila adult midgut

FBgn0260971

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG42594

Transgenic constructs containing regulatory region of CG42594

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene partially disrupted in

In(1)dl-49

(Miller et al., 2016)

Inferred from location ( 17 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

CG42594^GD15948

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

CG42594^GD3229

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG42594^GD3229

viable, with Scer\GAL4^Mef2.PR

CG42594^GD3229

viable, with Scer\GAL4^pnr-MD237

CG42594^GD3229

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (17)

Hsap\KCNK18

5 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (15)

Rnor\Kcnk18

5 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (16)

Mmus\Kcnk18

4 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\kcnk18

3 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\kcnk13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (21)

Drer\kcnk18

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-261a10.5

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (49)

Cele\twk-26

4 of 14

Yes

Cele\twk-45

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP003031

5 of 12

Yes

Agam\AgaP_AGAP013077

3 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\KCO1

4 of 13

Yes

Atha\KCO2

4 of 13

Yes

Atha\KCO5

4 of 13

Yes

Atha\KCO6

4 of 13

Yes

Atha\TPK4

4 of 13

Yes

Atha\KCO3

2 of 13

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\TOK1

4 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG42594. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (10)

6 of 13

6 of 13

6 of 13

6 of 13

5 of 13

5 of 13

5 of 13

4 of 13

4 of 13

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KCNK18; potassium two pore domain channel subfamily K member 18

5 of 14

613655

MIGRAINE WITH AURA, SUSCEPTIBILITY TO, 13; MGR13

KCNK5; potassium two pore domain channel subfamily K member 5

3 of 14

603493

KCNK17; potassium two pore domain channel subfamily K member 17

3 of 14

607370

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

1073105

DroID - A comprehensive database of gene and protein interactions.

FBgn0260971

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_stop_codon_read_through

(FlyBase Genome Annotators, 2014)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-9

Cytogenetic map

4D2-4D5

Sequence location

X:4,840,833..4,906,575 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

4D2-4D5

; Limits computationally determined from genome sequence between P{EP}CG2930^EP1352 and P{EP}EP425

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-9

(FlyBase, 2016)

1-9.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

12878

y¹ P{SUPor-P}CG42594^KG03323

25888

y¹ v¹; P{TRiP.JF01929}attP2

29593

y¹ v¹; P{TRiP.JF03272}attP2

50494

y¹ w^* Mi{MIC}CG42594^MI08920

35006

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01416}attP2

85935

y¹ sc^* v¹ sev²¹; P{TKO.GS05390}attP40/CyO

24795

y¹ P{EPgy2}CG32815^EY13020 w^67c23 P{EPgy2}CG42594^EY13020

6952R-1

15470R-3

v7042

w¹¹¹⁸; P{GD3229}v7042

v46415

w¹¹¹⁸; P{GD15948}v46415

Genomic Clones (35)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (4)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0260971

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG15470 CG32771 CG32770

(FlyBase Genome Annotators, 2009)

Additional comments

Annotations CG15470, CG32771 and CG32770 merged as CG42594 in release 5.18 of the genome annotation.

(FlyBase Genome Annotators, 2009)

Annotation CG6952 split into CG32770 and CG32771 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Name Synonyms

CG15470

CG32770

CG32771

Secondary FlyBase IDs

FBgn0029731
FBgn0052771
FBgn0029732
FBgn0052770

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

8674091

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

E1JJG7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

E1JJG7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

8674091/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260971

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260971

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42594

MARRVEL_MODEL - MARRVEL (model organism gene)

8674091

Linkouts

BioGRID - A database of protein and genetic interactions.

1073105

DroID - A comprehensive database of gene and protein interactions.

FBgn0260971

DRSC - Results frm RNAi screens

FBgn0260971

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG42594_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0260971

Flygut - An atlas of the Drosophila adult midgut

FBgn0260971

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0260971

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014979

References (42)

The following external sites may use different assemblies or annotations than FlyBase.

-0.58

Report Sections