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Gene Dmel\RfC4

General Information
Symbol	Dmel\RfC4	Species	D. melanogaster
Name	Replication factor C subunit 4	Annotation symbol	CG14999
Feature type	protein_coding_gene	FlyBase ID	FBgn0260985
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	Rfc40, l(3)e20, l(3)64Ai
Genomic Location
Chromosome (arm)	3L	Recombination map	3-
Cytogenetic map	64A7-64A7	Sequence location	3L:4,134,860..4,136,039 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Replication factor C subunit 4 is referred to in FlyBase by the symbol Dmel\RfC4 (CG14999, FBgn0260985). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATP-dependent DNA helicase activity. There is experimental evidence that it is involved in the biological process: mitotic chromosome condensation; female meiosis chromosome segregation; DNA replication checkpoint; lateral inhibition; mitosis; DNA damage checkpoint; neurogenesis. 11 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; polytene chromosome; chaeta; polytene chromosome band; cell cycle; imaginal disc; embryonic/larval brain. It has one annotated transcript and one annotated polypeptide. Protein features are: ATPase, AAA+ type, core; ATPase, AAA-type, core; DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal; Replication factor C. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1634943_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of RfC4. Gene sequence location is 3L:4134860..4136039.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	l(3)SG13 Homozygous larvae contain rudimentary imaginal discs, but disc primordia do not grow during larval development; testes and ovaries smaller than normal, and cell number in central nervous system reduced. Mutant gonads do not survive metamorphosis when implanted into wild-type larvae. Homozygous cuticular clones appear to develop normally. Mutant larvae support growth of implanted wild-type discs. Normal gene product postulated to be required for cell proliferation; survival of somatic epidermal clones attributed to perdurance. Larval ganglia exhibit extremely low mitotic index; chromosomes irregularly condensed; extensive chromosome fragmentation observed frequently (Gatti and Baker, 1989, Genes Dev. 3: 438-53); salivary chromosomes appear normal.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	Unknown western blot Controlled Vocabulary Terms larval stage embryonic/larval neuroblast third instar larval stage organism Sequence features HFA08691 BKN22927
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64A7-64A7   Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64A10-64A11     (Harrison, 1994.10.14) 64A10-64A11   (determined by in situ hybridisation)   (Harrison et al., 1995)
Experimentally Determined Recombination Data
Location	3-25.9 (Gatti and Baker, 1989) 3- (Harrison, 1994.10.14)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RfC4 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RfC4-RA FBtr0073264  NM_079191   1128   331
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RfC4-PA   FBpp0073120   37.2   331   7.82     NP_523915   AAF47843
Additional Polypeptide Data & Comments
Reported size (kDa)	331 (aa); 40 (kD observed) (Harrison et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ATPase, AAA+ type, core (IPR003593) DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal (IPR008921) Replication factor C (IPR013748) ATPase, AAA-type, core (IPR003959)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA540122   AE014296 AAF47843   AI520349   AW943506   AY094829 AAM11182   BF493261   BG633345   BG636208   BI564006   BI588376   BI633317   BI635475   BX026889   BX046199   BX058571   CK604234   CK604235   CK604264   CK604265   CK604376   CK604377   CK604473   CK604474   CK604738   CK604739   CO193287   CO308614   CO311589   CO323688   EC064883   EC080453   EC203783   EC234863   EC262984   EV577691   EV582777   GH944057   GH948569   GH953822   GH961756   GH961805   GH963547   GH968388   GH974410   GH975742   GH981340   U15967 AAB60241
UniProtKB/Swiss-Prot	P53034
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   embryonic/larval neuroblast   (Krause et al., 2001) Comment:proliferating neuroblasts western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Harrison et al., 1995) larval stage   organism   (Krause et al., 2001)
Additional Descriptive Data
	RfC4 protein is detected throughout embryogenesis on Western blots. It is detected in the nuclei of blastoderm stage embryos. (Harrison et al., 1995) RfC4 protein is distributed in zones of proliferation in the larval central nervous system. (Krause et al., 2001)
Marker for
Subcellular Localization
CV Term	nucleus (Harrison et al., 1995)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0260985    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0260985 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0260985 expression level Developmental Stage   Expression Level embryo 00-02hr    9211 embryo 02-04hr    1966 embryo 04-06hr    5578 embryo 06-08hr    3995 embryo 08-10hr    3018 embryo 10-12hr    1237 embryo 12-14hr    553 embryo 14-16hr    447 embryo 16-18hr    267 embryo 18-20hr    287 embryo 20-22hr    407 embryo 22-24hr    449 larva L1    1858 larva L2    1383 larva L3 12hr old    1019 larva L3 puffstage 1-2    1343 larva L3 puffstage 3-6    1807 larva L3 puffstage 7-9    1883 white prepupae new    1567 white prepupae 12hr    2858 white prepupae 24hr    1939 pupae 2d postWPP    718 pupae 3d postWPP    534 pupae 4d postWPP    762 adult male 01day    381 adult male 05day    476 adult male 30day    566 adult female 01day    3460 adult female 05day    5663 adult female 30day    5859 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (9211) embryo 02-04hr  (1966) embryo 04-06hr  (5578) embryo 06-08hr  (3995) embryo 08-10hr  (3018) embryo 10-12hr  (1237) embryo 12-14hr  (553) embryo 14-16hr  (447) embryo 16-18hr  (267) embryo 18-20hr  (287) embryo 20-22hr  (407) embryo 22-24hr  (449) larva L1  (1858) larva L2  (1383) larva L3 12hr old  (1019) larva L3 puffstage 1-2  (1343) larva L3 puffstage 3-6  (1807) larva L3 puffstage 7-9  (1883) white prepupae new  (1567) white prepupae 12hr  (2858) white prepupae 24hr  (1939) pupae 2d postWPP  (718) pupae 3d postWPP  (534) pupae 4d postWPP  (762) adult male 01day  (381) adult male 05day  (476) adult male 30day  (566) adult female 01day  (3460) adult female 05day  (5663) adult female 30day  (5859) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (9211) embryo 02-04hr  (1966) embryo 04-06hr  (5578) embryo 06-08hr  (3995) embryo 08-10hr  (3018) embryo 10-12hr    1237 embryo 12-14hr    553 embryo 14-16hr    447 embryo 16-18hr    267 embryo 18-20hr    287 embryo 20-22hr    407 embryo 22-24hr    449 larva L1  (1858) larva L2    1383 larva L3 12hr old    1019 larva L3 puffstage 1-2    1343 larva L3 puffstage 3-6  (1807) larva L3 puffstage 7-9  (1883) white prepupae new  (1567) white prepupae 12hr  (2858) white prepupae 24hr  (1939) pupae 2d postWPP    718 pupae 3d postWPP    534 pupae 4d postWPP    762 adult male 01day    381 adult male 05day    476 adult male 30day    566 adult female 01day  (3460) adult female 05day  (5663) adult female 30day  (5859) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9211 embryo 02-04hr    1966 embryo 04-06hr    5578 embryo 06-08hr    3995 embryo 08-10hr    3018 embryo 10-12hr    1237 embryo 12-14hr    553 embryo 14-16hr    447 embryo 16-18hr    267 embryo 18-20hr    287 embryo 20-22hr    407 embryo 22-24hr    449 larva L1    1858 larva L2    1383 larva L3 12hr old    1019 larva L3 puffstage 1-2    1343 larva L3 puffstage 3-6    1807 larva L3 puffstage 7-9    1883 white prepupae new    1567 white prepupae 12hr    2858 white prepupae 24hr    1939 pupae 2d postWPP    718 pupae 3d postWPP    534 pupae 4d postWPP    762 adult male 01day    381 adult male 05day    476 adult male 30day    566 adult female 01day    3460 adult female 05day    5663 adult female 30day    5859 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9211 embryo 02-04hr    1966 embryo 04-06hr    5578 embryo 06-08hr    3995 embryo 08-10hr    3018 embryo 10-12hr    1237 embryo 12-14hr    553 embryo 14-16hr    447 embryo 16-18hr    267 embryo 18-20hr    287 embryo 20-22hr    407 embryo 22-24hr    449 larva L1    1858 larva L2    1383 larva L3 12hr old    1019 larva L3 puffstage 1-2    1343 larva L3 puffstage 3-6    1807 larva L3 puffstage 7-9    1883 white prepupae new    1567 white prepupae 12hr    2858 white prepupae 24hr    1939 pupae 2d postWPP    718 pupae 3d postWPP    534 pupae 4d postWPP    762 adult male 01day    381 adult male 05day    476 adult male 30day    566 adult female 01day    3460 adult female 05day    5663 adult female 30day    5859 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0260985 expression level Developmental Stage   Expression Level embryo 00-02hr    9211 embryo 02-04hr    1966 embryo 04-06hr    5578 embryo 06-08hr    3995 embryo 08-10hr    3018 embryo 10-12hr    1237 embryo 12-14hr    553 embryo 14-16hr    447 embryo 16-18hr    267 embryo 18-20hr    287 embryo 20-22hr    407 embryo 22-24hr    449 larva L1    1858 larva L2    1383 larva L3 12hr old    1019 larva L3 puffstage 1-2    1343 larva L3 puffstage 3-6    1807 larva L3 puffstage 7-9    1883 white prepupae new    1567 white prepupae 12hr    2858 white prepupae 24hr    1939 pupae 2d postWPP    718 pupae 3d postWPP    534 pupae 4d postWPP    762 adult male 01day    381 adult male 05day    476 adult male 30day    566 adult female 01day    3460 adult female 05day    5663 adult female 30day    5859 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (9211) embryo 02-04hr  (1966) embryo 04-06hr  (5578) embryo 06-08hr  (3995) embryo 08-10hr  (3018) embryo 10-12hr  (1237) embryo 12-14hr  (553) embryo 14-16hr  (447) embryo 16-18hr  (267) embryo 18-20hr  (287) embryo 20-22hr  (407) embryo 22-24hr  (449) larva L1  (1858) larva L2  (1383) larva L3 12hr old  (1019) larva L3 puffstage 1-2  (1343) larva L3 puffstage 3-6  (1807) larva L3 puffstage 7-9  (1883) white prepupae new  (1567) white prepupae 12hr  (2858) white prepupae 24hr  (1939) pupae 2d postWPP  (718) pupae 3d postWPP  (534) pupae 4d postWPP  (762) adult male 01day  (381) adult male 05day  (476) adult male 30day  (566) adult female 01day  (3460) adult female 05day  (5663) adult female 30day  (5859) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (9211) embryo 02-04hr  1966 embryo 04-06hr  (5578) embryo 06-08hr  (3995) embryo 08-10hr  (3018) embryo 10-12hr    1237 embryo 12-14hr    553 embryo 14-16hr    447 embryo 16-18hr    267 embryo 18-20hr    287 embryo 20-22hr    407 embryo 22-24hr    449 larva L1  1858 larva L2    1383 larva L3 12hr old    1019 larva L3 puffstage 1-2    1343 larva L3 puffstage 3-6  1807 larva L3 puffstage 7-9  1883 white prepupae new  1567 white prepupae 12hr  (2858) white prepupae 24hr  1939 pupae 2d postWPP    718 pupae 3d postWPP    534 pupae 4d postWPP    762 adult male 01day    381 adult male 05day    476 adult male 30day    566 adult female 01day  (3460) adult female 05day  (5663) adult female 30day  (5859) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9211 embryo 02-04hr    1966 embryo 04-06hr    5578 embryo 06-08hr    3995 embryo 08-10hr    3018 embryo 10-12hr    1237 embryo 12-14hr    553 embryo 14-16hr    447 embryo 16-18hr    267 embryo 18-20hr    287 embryo 20-22hr    407 embryo 22-24hr    449 larva L1    1858 larva L2    1383 larva L3 12hr old    1019 larva L3 puffstage 1-2    1343 larva L3 puffstage 3-6    1807 larva L3 puffstage 7-9    1883 white prepupae new    1567 white prepupae 12hr    2858 white prepupae 24hr    1939 pupae 2d postWPP    718 pupae 3d postWPP    534 pupae 4d postWPP    762 adult male 01day    381 adult male 05day    476 adult male 30day    566 adult female 01day    3460 adult female 05day    5663 adult female 30day    5859 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9211 embryo 02-04hr    1966 embryo 04-06hr    5578 embryo 06-08hr    3995 embryo 08-10hr    3018 embryo 10-12hr    1237 embryo 12-14hr    553 embryo 14-16hr    447 embryo 16-18hr    267 embryo 18-20hr    287 embryo 20-22hr    407 embryo 22-24hr    449 larva L1    1858 larva L2    1383 larva L3 12hr old    1019 larva L3 puffstage 1-2    1343 larva L3 puffstage 3-6    1807 larva L3 puffstage 7-9    1883 white prepupae new    1567 white prepupae 12hr    2858 white prepupae 24hr    1939 pupae 2d postWPP    718 pupae 3d postWPP    534 pupae 4d postWPP    762 adult male 01day    381 adult male 05day    476 adult male 30day    566 adult female 01day    3460 adult female 05day    5663 adult female 30day    5859 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0260985    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0260985 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0260985 expression level Tissue   Expression Level Larval Central Nervous System    441.05 Larval Midgut    21.1 Larval Hindgut    74.7 Larval Malpighian Tubules    31.2 Larval Fat Body    34.5 Larval Salivary Gland    89.7 Larval Trachea    259.325 Larval Carcass    75.625 Adult Head    48.9 Adult Eye    57.4 Adult Brain    75.8 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    48.7 Adult Midgut    28.8 Adult Hindgut    50.3 Adult Malpighian Tubules    43.8 Adult Fat Body    31.7 Adult Salivary Gland    36.9 Adult Heart    37.25 Adult VirginFemale Spermatheca    24.8 Adult InseminatedFemale Spermatheca    39.9 Adult Ovary    628.3 Adult Testis    52.9 Adult Male Accessory Gland    106.7 Adult Carcass    37.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    441.05 Larval Midgut    21.1 Larval Hindgut    74.7 Larval Malpighian Tubules    31.2 Larval Fat Body    34.5 Larval Salivary Gland    89.7 Larval Trachea    259.325 Larval Carcass    75.625 Adult Head    48.9 Adult Eye    57.4 Adult Brain    75.8 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    48.7 Adult Midgut    28.8 Adult Hindgut    50.3 Adult Malpighian Tubules    43.8 Adult Fat Body    31.7 Adult Salivary Gland    36.9 Adult Heart    37.25 Adult VirginFemale Spermatheca    24.8 Adult InseminatedFemale Spermatheca    39.9 Adult Ovary  (628.3) Adult Testis    52.9 Adult Male Accessory Gland    106.7 Adult Carcass    37.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    441.05 Larval Midgut    21.1 Larval Hindgut    74.7 Larval Malpighian Tubules    31.2 Larval Fat Body    34.5 Larval Salivary Gland    89.7 Larval Trachea    259.325 Larval Carcass    75.625 Adult Head    48.9 Adult Eye    57.4 Adult Brain    75.8 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    48.7 Adult Midgut    28.8 Adult Hindgut    50.3 Adult Malpighian Tubules    43.8 Adult Fat Body    31.7 Adult Salivary Gland    36.9 Adult Heart    37.25 Adult VirginFemale Spermatheca    24.8 Adult InseminatedFemale Spermatheca    39.9 Adult Ovary    628.3 Adult Testis    52.9 Adult Male Accessory Gland    106.7 Adult Carcass    37.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    441.05 Larval Midgut    21.1 Larval Hindgut    74.7 Larval Malpighian Tubules    31.2 Larval Fat Body    34.5 Larval Salivary Gland    89.7 Larval Trachea    259.325 Larval Carcass    75.625 Adult Head    48.9 Adult Eye    57.4 Adult Brain    75.8 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    48.7 Adult Midgut    28.8 Adult Hindgut    50.3 Adult Malpighian Tubules    43.8 Adult Fat Body    31.7 Adult Salivary Gland    36.9 Adult Heart    37.25 Adult VirginFemale Spermatheca    24.8 Adult InseminatedFemale Spermatheca    39.9 Adult Ovary    628.3 Adult Testis    52.9 Adult Male Accessory Gland    106.7 Adult Carcass    37.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0260985 expression level Tissue   Expression Level Larval Central Nervous System    441.05 Larval Midgut    21.1 Larval Hindgut    74.7 Larval Malpighian Tubules    31.2 Larval Fat Body    34.5 Larval Salivary Gland    89.7 Larval Trachea    259.325 Larval Carcass    75.625 Adult Head    48.9 Adult Eye    57.4 Adult Brain    75.8 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    48.7 Adult Midgut    28.8 Adult Hindgut    50.3 Adult Malpighian Tubules    43.8 Adult Fat Body    31.7 Adult Salivary Gland    36.9 Adult Heart    37.25 Adult VirginFemale Spermatheca    24.8 Adult InseminatedFemale Spermatheca    39.9 Adult Ovary    628.3 Adult Testis    52.9 Adult Male Accessory Gland    106.7 Adult Carcass    37.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    441.05 Larval Midgut    21.1 Larval Hindgut    74.7 Larval Malpighian Tubules    31.2 Larval Fat Body    34.5 Larval Salivary Gland    89.7 Larval Trachea    259.325 Larval Carcass    75.625 Adult Head    48.9 Adult Eye    57.4 Adult Brain    75.8 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    48.7 Adult Midgut    28.8 Adult Hindgut    50.3 Adult Malpighian Tubules    43.8 Adult Fat Body    31.7 Adult Salivary Gland    36.9 Adult Heart    37.25 Adult VirginFemale Spermatheca    24.8 Adult InseminatedFemale Spermatheca    39.9 Adult Ovary  628.3 Adult Testis    52.9 Adult Male Accessory Gland    106.7 Adult Carcass    37.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    441.05 Larval Midgut    21.1 Larval Hindgut    74.7 Larval Malpighian Tubules    31.2 Larval Fat Body    34.5 Larval Salivary Gland    89.7 Larval Trachea    259.325 Larval Carcass    75.625 Adult Head    48.9 Adult Eye    57.4 Adult Brain    75.8 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    48.7 Adult Midgut    28.8 Adult Hindgut    50.3 Adult Malpighian Tubules    43.8 Adult Fat Body    31.7 Adult Salivary Gland    36.9 Adult Heart    37.25 Adult VirginFemale Spermatheca    24.8 Adult InseminatedFemale Spermatheca    39.9 Adult Ovary    628.3 Adult Testis    52.9 Adult Male Accessory Gland    106.7 Adult Carcass    37.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    441.05 Larval Midgut    21.1 Larval Hindgut    74.7 Larval Malpighian Tubules    31.2 Larval Fat Body    34.5 Larval Salivary Gland    89.7 Larval Trachea    259.325 Larval Carcass    75.625 Adult Head    48.9 Adult Eye    57.4 Adult Brain    75.8 Adult Thoracic-Abdominal Ganglion    85.6 Adult Crop    48.7 Adult Midgut    28.8 Adult Hindgut    50.3 Adult Malpighian Tubules    43.8 Adult Fat Body    31.7 Adult Salivary Gland    36.9 Adult Heart    37.25 Adult VirginFemale Spermatheca    24.8 Adult InseminatedFemale Spermatheca    39.9 Adult Ovary    628.3 Adult Testis    52.9 Adult Male Accessory Gland    106.7 Adult Carcass    37.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    48.9   NA  Eye    57.4   NA  Brain    75.8   441.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    85.6   NA  Crop    48.7   21.1  Midgut    28.8   74.7  Hindgut    50.3   31.2  Malpighian Tubules    43.8   34.5  Fat Body    31.7   89.7  Salivary Gland    36.9   NA  Heart    37.25   259.325  Trachea    NA   NA  VirginFemale Spermatheca    24.8   NA  InseminatedFemale Spermatheca    39.9   NA  Ovary    628.3   NA  Testis    52.9   NA  Male Accessory Gland    106.7   75.625  Carcass    37.7 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260985
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 RfC4GD4598 lethal | larval stage | recessive RfC4e20 lethal | pupal stage | recessive RfC4A18 RfC4B6 RfC4B12 RfC4C2 lethal | recessive RfC4A18 RfC4B6 RfC4Scim viable, with Scer\GAL4pnr-MD237 RfC4GD4598 Other Phenotypes Allele meiotic cell cycle defective | dominant RfC4Scim mitotic cell cycle defective RfC4A18 RfC4e20 RfC4Scim neuroanatomy defective | larval stage, with Scer\GAL41407 RfC4GD4598 visible, with Scer\GAL4pnr-MD237 RfC4GD4598 wild-type RfC4+t10.2 Phenotype manifest in Allele anaphase & condensed nuclear chromosome RfC4Scim cell cycle RfC4e20 chaeta, with Scer\GAL4pnr-MD237 RfC4GD4598 embryonic/larval brain RfC4A18 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 RfC4GD4598 ganglion mother cell | larval stage, with Scer\GAL41407 RfC4GD4598 imaginal disc RfC4e20 RfC4A18 RfC4B12 RfC4B6 RfC4C2 metaphase & condensed nuclear chromosome RfC4Scim mitosis & nuclear chromosome RfC4Scim polytene chromosome RfC4e20 RfC4A18 polytene chromosome band RfC4e20 RfC4A18 trichogen cell, with Scer\GAL4pnr-MD237 RfC4GD4598
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of RfC4 Class Mutagen Stocks Known lesion RfC4A18 2 Yes RfC4e20 ethyl methanesulfonate 2 Yes RfC4B6 1 -- RfC4NP4470 1 -- RfC4B12 0 -- RfC4C2 0 -- RfC4Scim Delta2-3 0 Yes RfC4unspecified 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RfC4 Class Mutagen Stocks Known lesion RfC4GD4598 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes RfC4KK102831 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RfC4+t10.2 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)BSC735 (Christensen et al., 2009.2.28) Df(3L)Exel6100 (Ryder, 2004.4.26) Df(3L)GN19 (Harrison et al., 1995) Df(3L)GN34 (Harrison et al., 1995) Df(3L)TE1 (Harrison et al., 1995)
Not disrupted in	Df(3L)C175 (Harrison et al., 1995) Df(3L)HR298 (Harrison et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK102831} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system P{GawB}RfC4NP4470 No miscellaneous insertions P{SUPor-P}RfC4Scim NA
Gene Ontology: Function, Process & Cellular Component ( 18 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
DNA damage checkpoint inferred from mutant phenotype (Krause et al., 2001) DNA replication checkpoint inferred from mutant phenotype (Krause et al., 2001) female meiosis chromosome segregation inferred from mutant phenotype (Dobie et al., 2001) lateral inhibition inferred from mutant phenotype (Mummery-Widmer et al., 2009) mitosis inferred from mutant phenotype (Dobie et al., 2001) mitotic chromosome condensation inferred from mutant phenotype (Dobie et al., 2001) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Krause et al., 2001)
Terms Based on Predictions or Assertions ( 11 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR013748 (FlyBase Curators et al., 2004-) ATP-dependent DNA helicase activity inferred from sequence or structural similarity with EMBL:AF139987 (FlyBase, 1992-) DNA binding non-traceable author statement (Krause et al., 1999, Harrison, 1994.10.14, Swiss-Prot Project Members, 1996.10.1) DNA clamp loader activity inferred from electronic annotation with InterPro:IPR013748 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
DNA repair traceable author statement (Mossi et al., 1997) DNA replication inferred from sequence or structural similarity with EMBL:AF139987 (FlyBase, 1992-) non-traceable author statement (Krause et al., 1999, Harrison, 1994.10.14) traceable author statement (Mossi et al., 1997) DNA strand elongation involved in DNA replication non-traceable author statement (Swiss-Prot Project Members, 1996.10.1) traceable author statement (Mossi et al., 1997) mitotic chromosome condensation non-traceable author statement (Heck and Krause, 1997) sister chromatid segregation non-traceable author statement (Heck and Krause, 1997)
Cellular Component
CV term Evidence References
DNA replication factor C complex inferred from sequence or structural similarity with EMBL:AF139987 (FlyBase, 1992-) non-traceable author statement (Krause et al., 1999, Harrison, 1994.10.14) nucleus non-traceable author statement (Swiss-Prot Project Members, 1996.10.1)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RfC4 allele Gene References RfC40B6 cutlet (Jaffe and Jongens, 2001) RfC40unspecified nab (Clements et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 63972 DroID - A comprehensive database of gene and protein interactions. • FBgn0260985 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38492
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23886 Dere\GG14218 Dgri\GH15919 Dmoj\GI16571 Dper\GL12737 Dpse\GA13416 Dsec\GM14011 Dsim\GD13291 Dvir\GJ12823 Dwil\GK10084 Dyak\GE20646
Curated drosophilid orthologs	Dpse\GA13416   Dsim\RfC40 (Swanson et al., 2001)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL006223-PA Aedes aegypti (mosquito) AGAP007477-PA Anopheles gambiae (mosquito) XP_623937.1 Apis mellifera (honey bee) XP_001945771.1 Acyrthosiphon pisum (pea aphid) At1g63160.1 Arabidopsis thaliana (wall cress) 86526 Batrachochytrium dendrobatidis (chytrid fungus) 123851 Branchiostoma floridae (lancelet) 123856 Branchiostoma floridae (lancelet) XP_001899751.1 Brugia malayi (roundworm nematode) BGIBMGA009495-PA Bombyx mori (silk worm) ENSBTAP00000024740 Bos taurus (bovine) CAL0000922 Candida albicans (a form of yeast) CN27463 Caenorhabditis brenneri (small nematode) CBP36817 Caenorhabditis briggsae (nematode) CE17139 Caenorhabditis elegans (worm) ENSCAFP00000017619 Canis familiaris (dog) CAGL0I07645g Candida glabrata (haploid yeast) Chro.30175 Cryptosporidium hominis (obligate parasite) ENSCINP00000010059 Ciona intestinalis (sea squirt) JA13655 Caenorhabditis japonica (nematode) CMF110C Cyanidioschyzon merolae (red alga) cn07232 Cryptococcus neoformans (yeast-like fungus) cgd3_1450 Cryptosporidium parvum (protozoan parasite) CPIJ000302 Culex pipens (mosquito) ENSCPOP00000011113 Cavia porcellus (guinea pig) 185418 Chlamidomonas reinhardtii (green alga) RP24141 Caenorhabditis remanei (nematode) ENSCSAVP00000003175 Ciona savignyi (sedentary tunicate) 192756 Capitella spl (polychaete worm) DDB0232231 Dictyostelium discoideum (amoeba) DEHA2A13574g Debaryomyces Hansenii (yeast) 303531 Daphnia pulex (daphnia) ENSDARP00000016472 Danio rerio (zebra fish) ENSECAP00000000368 Equus caballus (horse) NP_415412.1 Escherichia coli K12 (bacteria) XP_651156 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000000614 Gymnopilus aculeatus (mushroom) ENSGALP00000001978 Gallus gallus (chicken) GL50803_14095 Giardia lamblia (flagellated protozoan) 186210 Hypsipyla robusta (cedar shoot borer) ENSP00000055077 Homo sapiens (human) ISCW024706-PA Ixodes scapularis (deer tick) KLLA0C03718g Kluyveromyces lactis (yeast) 228440 Lottia gigantea (owl limpet) LmjF27.0550 Leishmania major (virus) 23144 Monocosta brevicollis (choanoflagellate) ENSMODP00000023216 Monodelphis domestica (gray opossum) ENSMMUP00000005124 Macaca mulatta (rhesus monkey) ENSMUSP00000023867 Mus musculus (house mouse) 209082 Nematostella vectensis (starlet anemone) XP_001607747.1 Nasonia vitripennis (wasp) ENSOANP00000011669 Ornithorynchus anatinus (platypus) ENSORLP00000023491 Oryzias latipes (japanese killifish) LOC_Os04g48060.1 Oryza sativa (asian rice) 20272 Ostreococcus tauri (green alga) PFL2005w Plasmodium falciparum (protozoal parasite) PHUM005360-PA Pediculus humanus (human body louse) PP00031 Pristionchus pacificus (nematode) 112407 Physcomitrella patens (moss) ENSPPYP00000019603 Pongo pygmaeus (orangutan) 71119 Phytophthora ramorum (sudden oak death pathogen) 108506 Phytophthora sojae (root rot pathogen) 817339 Populus trichocarpa (black cottonwood) ENSPTRP00000032986 Pan troglodytes (chimpanzee) PVX_100985 Plasmodium vivax (protozoal parasite) ENSRNOP00000055996 Rattus norvegicus (norway rat) 4830755 Sorghum bicolor (sorghum) YOL094C Saccharomyces cerevisiae (budding yeast) Smp_140530 Schistosoma mansoni (trematode parasite) SPAC1687.03c Schizosaccharomyces pombe (fission yeast) XP_790650.2 Strongylocentrotus purpuratus (purple sea urchin) 28707 Trichoplax adhaerens (metazoa) TA04525 Theileria annulata (apicomplexan parasite) XP_967947.1 Tribolium castaneum (red flour beetle) Tc00.1047053511589.160 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000018004 Tetraodon nigroviridis (green spotted pufferfish) TP03_0432 Theileria parva (parasitic protozoan) 8830 Thalassiosira pseudonana (marine diatom) ENSTRUP00000024932 Takifugu rubripes (pufferfish Takifugu) 3738.m00033 Tetrahymena thermophila (ciliate protozoa) 94370.m00027 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000039544 Xenopus tropicalis (frog) YALI0F07337g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB12474 NV10339 LH14131 ISCW024706 FBgn0237951 FBgn0212099 FBgn0200058 FBgn0185010 FBgn0168940 JGI_V11_303531 FBgn0073452 FBgn0150343 FBgn0139319 FBgn0123390 FBgn0106479 FBgn0100880 CPIJ000302 BGIBMGA009495 ACYPI007996 GB16926 AGAP007477 AAEL006223 PHUM237330 GLEAN_10228
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	6195 RfC4e20/TM1 5158 bw1; RfC4B6 st1/TM6B, Tb1 5157 bw1; RfC4A18 st1/TM6B, Tb1 8987 mwh1 RfC4e20 red1 e1/TM3, Sb1 Ser1, y+
Kyoto	108081 bw1; RfC4A18 st1/TM6B, Tb1 104687 y* w*; P{GawB}RfC4NP4470 / TM6, P{UAS-lacZ.UW23-1}UW23-1
VDRC	v103930 P{KK102831}VIE-260B v37756 w1118; P{GD4598}v37756 v37757 w1118; P{GD4598}v37757
Genomic Clones ( 1 )
	BACR48M07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 35 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD40483
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01577 GH24992 LD19503 RH29569 RH34972 SD13818 SD16841 SD27378
Other clones	60489 102410 156055 525742 CK01.32B.G9 Dmel_dig_002743_0623_3743 Dmel_dig_012058_0612_1552 EC35537 EK191144 EK225512 EK258853 RP004123677 RP004197115 RP004232420 RP004245848 RP004335848 RP004364414 RP004455299 RP004513609 RP004531838 RP004533848 UT01023 UT01047 UT01137 UT01217 UT01418
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0260985 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38492 249487
Antibody Information
	monoclonal (Harrison et al., 1995)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: l(3)dsl1 l(3)SG13 (Shearn, 1995.5.3) Source for merge of: RfC4 RfC40 l(3)SG13 (Krause et al., 2001)
Additional comments
Other Comments
	Identified with: GH24992 <up>FlyBase curator comment: EST subsequently found to be chimeric</up>. (Zou et al., 2000) Candidate gene for tibia length quantitative trait locus. (Macdonald and Goldstein, 1999) EST GH24992 is chimeric; the 5' portion (AI405079) corresponds to part of SP2637, the 3' portion (BG633345) corresponds to part of RfC40. (FlyBase, 1996-) Clonal analysis demonstrates disc proliferation is reduced in mutant individuals. Discussed during genetic analysis of the Ras64B-Rop region. (Harrison et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079191 RefSeq (Proteins) • NP_523915 DDBJ / EMBL / GenBank • AAB60241 AAF47843 AAM11182 AI520349 AW943506 AY094829 BG633345 U15967 Entrez Gene - A searchable database of RefSeq genes. • 38492 UniProtKB/Swiss-Prot • P53034
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • ATPase, AAA+ type, core (IPR003593) DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal (IPR008921) Replication factor C (IPR013748) ATPase, AAA-type, core (IPR003959)
Linkouts
	BioGRID - A database of protein and genetic interactions • 63972 DroID - A comprehensive database of gene and protein interactions. • FBgn0260985 DRSC - Results from RNAi screens. • FBgn0260985 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260985 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0260985 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38492 249487 InParanoid A subset of ortholog calls from InParanoid. • AAEL006223-PA Aedes aegypti (mosquito) AGAP007477-PA Anopheles gambiae (mosquito) XP_623937.1 Apis mellifera (honey bee) XP_001945771.1 Acyrthosiphon pisum (pea aphid) At1g63160.1 Arabidopsis thaliana (wall cress) 86526 Batrachochytrium dendrobatidis (chytrid fungus) 123851 Branchiostoma floridae (lancelet) 123856 Branchiostoma floridae (lancelet) XP_001899751.1 Brugia malayi (roundworm nematode) BGIBMGA009495-PA Bombyx mori (silk worm) ENSBTAP00000024740 Bos taurus (bovine) CAL0000922 Candida albicans (a form of yeast) CN27463 Caenorhabditis brenneri (small nematode) CBP36817 Caenorhabditis briggsae (nematode) CE17139 Caenorhabditis elegans (worm) ENSCAFP00000017619 Canis familiaris (dog) CAGL0I07645g Candida glabrata (haploid yeast) Chro.30175 Cryptosporidium hominis (obligate parasite) ENSCINP00000010059 Ciona intestinalis (sea squirt) JA13655 Caenorhabditis japonica (nematode) CMF110C Cyanidioschyzon merolae (red alga) cn07232 Cryptococcus neoformans (yeast-like fungus) cgd3_1450 Cryptosporidium parvum (protozoan parasite) CPIJ000302 Culex pipens (mosquito) ENSCPOP00000011113 Cavia porcellus (guinea pig) 185418 Chlamidomonas reinhardtii (green alga) RP24141 Caenorhabditis remanei (nematode) ENSCSAVP00000003175 Ciona savignyi (sedentary tunicate) 192756 Capitella spl (polychaete worm) DDB0232231 Dictyostelium discoideum (amoeba) DEHA2A13574g Debaryomyces Hansenii (yeast) 303531 Daphnia pulex (daphnia) ENSDARP00000016472 Danio rerio (zebra fish) ENSECAP00000000368 Equus caballus (horse) NP_415412.1 Escherichia coli K12 (bacteria) XP_651156 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000000614 Gymnopilus aculeatus (mushroom) ENSGALP00000001978 Gallus gallus (chicken) GL50803_14095 Giardia lamblia (flagellated protozoan) 186210 Hypsipyla robusta (cedar shoot borer) ENSP00000055077 Homo sapiens (human) ISCW024706-PA Ixodes scapularis (deer tick) KLLA0C03718g Kluyveromyces lactis (yeast) 228440 Lottia gigantea (owl limpet) LmjF27.0550 Leishmania major (virus) 23144 Monocosta brevicollis (choanoflagellate) ENSMODP00000023216 Monodelphis domestica (gray opossum) ENSMMUP00000005124 Macaca mulatta (rhesus monkey) ENSMUSP00000023867 Mus musculus (house mouse) 209082 Nematostella vectensis (starlet anemone) XP_001607747.1 Nasonia vitripennis (wasp) ENSOANP00000011669 Ornithorynchus anatinus (platypus) ENSORLP00000023491 Oryzias latipes (japanese killifish) LOC_Os04g48060.1 Oryza sativa (asian rice) 20272 Ostreococcus tauri (green alga) PFL2005w Plasmodium falciparum (protozoal parasite) PHUM005360-PA Pediculus humanus (human body louse) PP00031 Pristionchus pacificus (nematode) 112407 Physcomitrella patens (moss) ENSPPYP00000019603 Pongo pygmaeus (orangutan) 71119 Phytophthora ramorum (sudden oak death pathogen) 108506 Phytophthora sojae (root rot pathogen) 817339 Populus trichocarpa (black cottonwood) ENSPTRP00000032986 Pan troglodytes (chimpanzee) PVX_100985 Plasmodium vivax (protozoal parasite) ENSRNOP00000055996 Rattus norvegicus (norway rat) 4830755 Sorghum bicolor (sorghum) YOL094C Saccharomyces cerevisiae (budding yeast) Smp_140530 Schistosoma mansoni (trematode parasite) SPAC1687.03c Schizosaccharomyces pombe (fission yeast) XP_790650.2 Strongylocentrotus purpuratus (purple sea urchin) 28707 Trichoplax adhaerens (metazoa) TA04525 Theileria annulata (apicomplexan parasite) XP_967947.1 Tribolium castaneum (red flour beetle) Tc00.1047053511589.160 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000018004 Tetraodon nigroviridis (green spotted pufferfish) TP03_0432 Theileria parva (parasitic protozoan) 8830 Thalassiosira pseudonana (marine diatom) ENSTRUP00000024932 Takifugu rubripes (pufferfish Takifugu) 3738.m00033 Tetrahymena thermophila (ciliate protozoa) 94370.m00027 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000039544 Xenopus tropicalis (frog) YALI0F07337g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38492 modMine - Data generated by the modENCODE project. • FBgn0260985 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB12474 NV10339 LH14131 ISCW024706 FBgn0237951 FBgn0212099 FBgn0200058 FBgn0185010 FBgn0168940 JGI_V11_303531 FBgn0073452 FBgn0150343 FBgn0139319 FBgn0123390 FBgn0106479 FBgn0100880 CPIJ000302 BGIBMGA009495 ACYPI007996 GB16926 AGAP007477 AAEL006223 PHUM237330 GLEAN_10228
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	154910_at (Kristensen et al., 2005) BEST:GH24992   CG14999 (Krause et al., 2001, Keleman et al., 2009.8.5, Gandhi et al., 2007, Hughes et al., 2008) DmRFC4 (Krause et al., 1999) l(3)64Ai (Clements et al., 2003, Harrison et al., 1995) l(3)A1   l(3)dsl-1 (Cohen, 1993) l(3)dsl1   l(3)e20 (Krause et al., 2001, Krause and Heck, 1996, Heck and Krause, 1997, Gatti and Baker, 1989, Ripoll et al., 1987, ) l(3)Rfc4 (Krause et al., 2001) l(3)rfc40 (Jaffe and Jongens, 2001) l(3)SG13   l(3)SH17 (Harrison et al., 1995) rfc (Harrison et al., 1994) RfC4 (Avet-Rochex et al., 2010) Rfc4 (Krause et al., 2001) rfc4 (Dobie et al., 2001) Rfc40 (Clements et al., 2003, Orian et al., 2003, ) RfC40 (Cayirlioglu et al., 2003, Somma et al., 2008, Christensen et al., 2009.2.28, Christensen et al., 2009.2.28, Neumüller et al., 2011) rfc40 (Harrison, 1994.10.14) rfc40 (Harrison, 1999.5.12)
Name Synonym	lethal (3) SG13   Replication-factor-C 40kD subunit   Replication factor C subunit 4
Secondary FlyBase IDs
	FBgn0002460 FBgn0004708 FBgn0015287 FBgn0026776 FBgn0043808
References ( 54 )
Generate a list of	All references relating to this gene ( 54 )
List References by type	Research paper  ( 23 ) Supplementary material  ( 6 ) Review  ( 4 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 5 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Book  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 2 )
	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Avet-Rochex et al., 2010, BMC Dev. Biol. 10: 65 An in vivo RNA interference screen identifies gene networks controlling Drosophila melanogaster blood cell homeostasis. [FBrf0211137] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...