DHR39, ftz-F1β, fs(2)neo8, fs(2)04443, NR5B1
nuclear receptor superfamily - master regulator of a program for the production of female reproductive glands and other secretory tissues
Please see the JBrowse view of Dmel\Hr39 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
5.0, 3.5 (northern blot); 2.734 (longest cDNA)
5.1, 3.5 (northern blot)
808, 701 (aa)
Monomer; forms a complex with ftz.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hr39 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Hr39 expression was studied in staged third instar larvae and prepupae collected at two hour intervals. Transcripts can first be detected in early third instar larvae and continue to be present at low levels until 106-108hr. After that, they accumulate to high levels, peaking in early prepupae. Hr39 transcripts drop in abundance in mid prepupae, rise again in 10hr prepupae, and continue to be present through the beginning of pupal development. In staged prepupal salivary glands, Hr39 transcripts are abundant at 0hr, decline in 2hr glands, and then are reintroduced, peaking 6-8hr after puparium formation. Hr39 transcripts are rapidly induced by ecdysone.
Hr39 3.5kb transcripts are most abundant in 0-3 hour embryos and are probably maternally inherited.
5.1kb Hr39 transcripts are not present in early embryos but are detected at all other stages of development. An elevated transcript level is observed during puparium formation. Transcripts are uniformly distributed in early embryos. At later stages, high levels of expression are observed in the ventral cord, brain, and hindgut.
3.5kb Hr39 transcripts are maternally expressed. They are uniformly distributed in early embryos.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Hr39 in JBrowsePlease Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Hr39 is involved in αbgr; axon guidance and fasciculation but is necessarily downregulated in γ axons for pruning to occur.
Hr39 does not play an essential role during development.
Temporal profile of gene expression is not altered in Eip74EF mutant background.
ftz-f1 and Hr39 bind as monomers to oligonucleotides corresponding to the ftz-f1 recognition element (F1RE) located within the zebra element of ftz promoter. Antagonism between the two receptors contributes to the net F1RE-dependent transcription of a reporter gene in cotransfection assays. Results suggest common target genes may be coregulated at the transcriptional level by a mechanism of competition between ftz-f1 and Hr39 monomers for binding to a common element.
Hr39 cloned by screening an expression library with oligonucleotide probe corresponding to the positive element of the Adh adult enhancer: DNA sequence analysis showed Hr39 belongs to the steroid hormone receptor superfamily. Whereas ftz-f1, which binds to the same sequence, acts as an activator of distal Adh transcription, Hr39 acts to repress distal Adh transcription.
Source for merge of: Hr39 fs(2)neo8 fs(2)04443
