FB2021_01, released Feb 18, 2021

Gene: Dmel\inaE

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General Information
Symbol
Dmel\inaE
Species
D. melanogaster
Name
inactivation no afterpotential E
Annotation Symbol
CG33174
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261244
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Snapshot
inactivation no afterpotential E (inaE) encodes a diacylglycerol lipase involved in phototransduction and response to oxidative stress. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

DAGL, diacylglycerol lipase

Key Links
Genomic Location
Cytogenetic map
12C4-12C5
Sequence location
X:13,783,714..13,811,493 [+]
Recombination map

1-46

RefSeq locus
NC_004354 REGION:13783714..13811493
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables lipoprotein lipase activity
inferred from direct assay
(Leung et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (8 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in phototransduction
inferred from mutant phenotype
(Leung et al., 2008)
involved_in response to oxidative stress
inferred from mutant phenotype
(Lin et al., 2014)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
involved_in endocannabinoid signaling pathway
inferred from biological aspect of ancestor with PANTHER:PTN001851551
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in G protein-coupled glutamate receptor signaling pathway
inferred from biological aspect of ancestor with PANTHER:PTN001851551
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in lipid metabolic process
inferred from electronic annotation with InterPro:IPR002921
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
involved_in neurogenesis
inferred from biological aspect of ancestor with PANTHER:PTN001851551
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in neurotransmitter biosynthetic process
inferred from biological aspect of ancestor with PANTHER:PTN001851551
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in retrograde trans-synaptic signaling by endocannabinoid
inferred from biological aspect of ancestor with PANTHER:PTN001851551
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Leung et al., 2008)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in postsynaptic membrane
inferred from biological aspect of ancestor with PANTHER:PTN001851551
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
TRIGLYCERIDE LIPASES -
Triglyceride lipases catalyze the hydrolysis of triacylglycerol into diacylglycerol and a carboxylate.
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
inaE (J.C. Hall)
Same basic eye-physiology phenotype as in inaA, inaB, and inaC; one allele (at least, i.e. inaE1) causes age-dependent photoreceptor degeneration.
Summary (Interactive Fly)

diacylglycerol lipase involved in visual signal transduction and lipid metabolism

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
6

Please see the JBrowse view of Dmel\inaE for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
inaE-RA
FBtr0073826
NM_176728
4004
644
inaE-RD
FBtr0073825
NM_176727
5910
737
inaE-RE
FBtr0290302
NM_001144723
5086
1318
inaE-RF
FBtr0290303
NM_001144724
5698
815
inaE-RG
FBtr0340290
NM_001298301
5062
1310
inaE-RH
FBtr0340291
NM_001298302
5973
729
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
inaE-PA
FBpp0073657
73.4
644
8.28
NP_788901
AAO41653
inaE-PD
FBpp0073656
83.9
737
8.43
NP_788900
AAO41652
inaE-PE
FBpp0288741
144.9
1318
7.64
NP_001138195
ACL82927
inaE-PF
FBpp0288742
92.4
815
7.89
NP_001138196
ACL82928
inaE-PG
FBpp0309251
143.9
1310
7.57
NP_001285230
AHN59700
inaE-PH
FBpp0309252
82.9
729
8.36
NP_001285231
AHN59701
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\inaE using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Leung et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\inaE in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of inaE
Transgenic constructs containing regulatory region of inaE
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation
Gene disrupted in
Df(1)AR10
(Leung et al., 2008)
Df(1)benCO2
(Leung et al., 2008)
Inferred from location
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\DAGLA
13 of 15
Yes
Yes
Hsap\DAGLB
5 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Dagla
13 of 15
Yes
Yes
Mmus\Daglb
4 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Dagla
11 of 13
Yes
Yes
Rnor\Daglb
3 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\dagla
11 of 12
Yes
Yes
Xtro\daglb
2 of 12
No
Yes
Xtro\LOC100486956
2 of 12
No
Yes
Xtro\LOC105945148
1 of 12
No
Yes
Danio rerio (Zebrafish) (2)
Drer\dagla
11 of 15
Yes
Yes
Drer\daglb
4 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\dagl-2
10 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (7)
Atha\AT1G05790
4 of 9
Yes
Yes
Atha\AT2G42450
2 of 9
No
Yes
Atha\AT3G14075
2 of 9
No
Yes
Atha\AT3G49050
1 of 9
No
Yes
Atha\AT4G00500
1 of 9
No
Yes
Atha\AT4G16070
1 of 9
No
Yes
Atha\AT5G37715
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900YH )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00008405
Drosophila simulans
Dsim\GD17147
Drosophila sechellia
Dsec\GM17642
Drosophila erecta
Dere\GG17815
Drosophila yakuba
Dyak\GE17110
Drosophila ananassae
Dana\GF19211
Drosophila pseudoobscura pseudoobscura
Dpse\GA26665
Drosophila persimilis
Dper\GL20159
Drosophila willistoni
Dwil\GK10079
Drosophila virilis
Dvir\GJ18541
Drosophila mojavensis
Dmoj\GI21769
Drosophila grimshawi
Dgri\GH12822
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500GS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17010296
Glossina morsitans
Tsetse fly
Gmor\GMOY004720
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08956
Mayetiola destructor
Hessian fly
Mdes\Mdes005156
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL018147
Anopheles darlingi
American malaria mosquito
Adar\ADAC000663
Anopheles gambiae
Malaria mosquito
Agam\AGAP000945
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ015557
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02W7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Apis florea
Little honeybee
Aflo\2683378212
Apis mellifera
Western honey bee
Amel\GB52082
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP25743
Bombus terrestris
Buff-tailed bumblebee
Bter\15213341
Linepithema humile
Argentine ant
Lhum\LH14360
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001b05
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG017097
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10124
Tribolium castaneum
Red flour beetle
Tcas\TC006897
Tribolium castaneum
Red flour beetle
Tcas\TC033401
Pediculus humanus
Human body louse
Phum\PHUM569760
Rhodnius prolixus
Kissing bug
Rpro\RPRC000586
Rhodnius prolixus
Kissing bug
Rpro\RPRC008859
Rhodnius prolixus
Kissing bug
Rpro\RPRC004456
Cimex lectularius
Bed bug
Clec\CLEC000819
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_16064
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_05132
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X028M )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR012868
Strigamia maritima
European centipede
Smar\SMAR011011
Ixodes scapularis
Black-legged tick
Isca\ISCW009207
Ixodes scapularis
Black-legged tick
Isca\ISCW007230
Ixodes scapularis
Black-legged tick
Isca\ISCW004765
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77722.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67601.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67603.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77723.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77720.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67967.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM67606.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur09g05290
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_306186
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_58603
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04AM )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Daglb
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Dagla
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Daglb-2
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000006466
Ciona intestinalis
Vase tunicate
Cint\LOC100175676
Gallus gallus
Domestic chicken
Ggal\DAGLA
Gallus gallus
Domestic chicken
Ggal\DAGLB
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    DAGLA; diacylglycerol lipase alpha
    13 of 15
    614015
        DAGLB; diacylglycerol lipase beta
        5 of 15
        614016
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Interactions Browser
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            pnr
            suppressible
            inaE
            (Pena-Rangel et al., 2002)
            trp
            enhanceable
            inaE
            (Leung et al., 2008)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Linkouts
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            X
            Recombination map

            1-46

            Cytogenetic map
            12C4-12C5
            Sequence location
            X:13,783,714..13,811,493 [+]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            12C4-12C5
            Limits computationally determined from genome sequence between P{EP}CG33174EP1101 and P{EP}EP1595EP1595
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            1-46

            (FlyBase, 2016)

            1-44.0

            (Comeron et al., 2012)

            1-

            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (12)
            Genomic Clones (20)
            cDNA Clones (80)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Laboratory Generated Antibodies
               

              polyclonal

              (Leung et al., 2008)
              Commercially Available Antibodies
               
              Other Information
              Relationship to Other Genes
              Source for database identify of
              Source for database merge of

              Source for merge of: inaE CG33174

              (Leung et al., 2008)

              Source for merge of: CG11102 CG11104 CG15761 CG11096

              (FlyBase Genome Annotators, 2002-2003)
              Additional comments

              Annotations CG11102, CG11104, CG15761 and CG11096 merged as CG33174 in release 3 of the genome annotation.

              (FlyBase Genome Annotators, 2002-2003)
              Other Comments
              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 62 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              FlyMine - An integrated database for Drosophila genomics
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              modMine - A data warehouse for the modENCODE project
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Synonyms and Secondary IDs (11)
              Reported As
              Symbol Synonym
              CG11096
              CG11102
              (Robertson, 2001.1.8)
              CG11104
              CG15761
              CG32630
              CG33174
              (Ashwal-Fluss et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5, Perkins et al., 2009, Chen et al., 2008, Haussmann et al., 2008, Leung et al., 2008, McPartland et al., 2006)
              DAGL
              (Johnson and Stolzing, 2019, Lin et al., 2014)
              EP(X)1101
              (Pena-Rangel et al., 2002)
              inaE
              (Katz and Minke, 2018, Crocker et al., 2016, Sarov et al., 2016, Lin et al., 2014, Pak et al., 2012, Leung et al., 2008)
              Name Synonyms
              CG33174
              diacylglycerol lipase
              (Johnson and Stolzing, 2019, Lin et al., 2014)
              inactivation no afterpotential E
              Secondary FlyBase IDs
              • FBgn0001264
              • FBgn0053174
              • FBgn0030523
              • FBgn0030526
              • FBgn0030527
              • FBgn0040866
              • FBgn0052630
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (44)