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Gene Dmel\sing

General Information
Symbol	Dmel\sing	Species	D. melanogaster
Name	singles bar	Annotation symbol	CG13011
Feature type	protein_coding_gene	FlyBase ID	FBgn0261245
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	X	Recombination map	1-
Cytogenetic map	15A-15A	Sequence location	X:16,573,330..16,575,817 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene singles bar is referred to in FlyBase by the symbol Dmel\sing (CG13011, FBgn0261245). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: myoblast fusion. 7 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; embryonic myoblast; A1-7 dorsal acute muscle 1; embryonic/larval somatic muscle. It has one annotated transcript and one annotated polypeptide. Protein features are: MARVEL-like domain; Marvel. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1627808_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of sing. Gene sequence location is X:16573330..16575817.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Clones FI15330
FB2012_01	Sequence features HFA19530 BKN30046
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 15A-15A   Left limit from (method unavailable) (FBrf0101712) Right limit from (method unavailable) (FBrf0101712)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 15A-15A     (Maeland et al., 1998)
Experimentally Determined Recombination Data
Location	1- (Maeland et al., 1996)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\sing for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) sing-RA FBtr0074318  NM_132918   2271   176
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein sing-PA   FBpp0074092   19.5   176   8.61     NP_573146   AAF48643
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Marvel (IPR008253) MARVEL-like domain (IPR021128)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014298 AAF48643   BT100197   BT128825   CO316147   EC065406   EW713157   GH943519   GH949050   GH950634   GH950719   GH952498   GH954441   GH959674   GH962921   GH965200   GH965776   GH971386   GH979101   GH981519   GH982664   GH983157   GH983371   GH984294   GH985120
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VXD1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0261245    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0261245 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0261245 expression level Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    267 embryo 04-06hr    733 embryo 06-08hr    3058 embryo 08-10hr    3909 embryo 10-12hr    3343 embryo 12-14hr    1103 embryo 14-16hr    521 embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9    117 white prepupae new    188 white prepupae 12hr    754 white prepupae 24hr    1602 pupae 2d postWPP    408 pupae 3d postWPP    90 pupae 4d postWPP    150 adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr  (267) embryo 04-06hr  (733) embryo 06-08hr  (3058) embryo 08-10hr  (3909) embryo 10-12hr  (3343) embryo 12-14hr  (1103) embryo 14-16hr  (521) embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9  (117) white prepupae new  (188) white prepupae 12hr  (754) white prepupae 24hr  (1602) pupae 2d postWPP  (408) pupae 3d postWPP    90 pupae 4d postWPP  (150) adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    267 embryo 04-06hr    733 embryo 06-08hr  (3058) embryo 08-10hr  (3909) embryo 10-12hr  (3343) embryo 12-14hr    1103 embryo 14-16hr    521 embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9    117 white prepupae new    188 white prepupae 12hr    754 white prepupae 24hr  (1602) pupae 2d postWPP    408 pupae 3d postWPP    90 pupae 4d postWPP    150 adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    267 embryo 04-06hr    733 embryo 06-08hr    3058 embryo 08-10hr    3909 embryo 10-12hr    3343 embryo 12-14hr    1103 embryo 14-16hr    521 embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9    117 white prepupae new    188 white prepupae 12hr    754 white prepupae 24hr    1602 pupae 2d postWPP    408 pupae 3d postWPP    90 pupae 4d postWPP    150 adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    267 embryo 04-06hr    733 embryo 06-08hr    3058 embryo 08-10hr    3909 embryo 10-12hr    3343 embryo 12-14hr    1103 embryo 14-16hr    521 embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9    117 white prepupae new    188 white prepupae 12hr    754 white prepupae 24hr    1602 pupae 2d postWPP    408 pupae 3d postWPP    90 pupae 4d postWPP    150 adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0261245 expression level Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    267 embryo 04-06hr    733 embryo 06-08hr    3058 embryo 08-10hr    3909 embryo 10-12hr    3343 embryo 12-14hr    1103 embryo 14-16hr    521 embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9    117 white prepupae new    188 white prepupae 12hr    754 white prepupae 24hr    1602 pupae 2d postWPP    408 pupae 3d postWPP    90 pupae 4d postWPP    150 adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr  (267) embryo 04-06hr  (733) embryo 06-08hr  (3058) embryo 08-10hr  (3909) embryo 10-12hr  (3343) embryo 12-14hr  (1103) embryo 14-16hr  (521) embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9  117 white prepupae new  (188) white prepupae 12hr  (754) white prepupae 24hr  (1602) pupae 2d postWPP  (408) pupae 3d postWPP    90 pupae 4d postWPP  (150) adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    267 embryo 04-06hr    733 embryo 06-08hr  (3058) embryo 08-10hr  (3909) embryo 10-12hr  (3343) embryo 12-14hr    1103 embryo 14-16hr    521 embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9    117 white prepupae new    188 white prepupae 12hr    754 white prepupae 24hr  1602 pupae 2d postWPP    408 pupae 3d postWPP    90 pupae 4d postWPP    150 adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    267 embryo 04-06hr    733 embryo 06-08hr    3058 embryo 08-10hr    3909 embryo 10-12hr    3343 embryo 12-14hr    1103 embryo 14-16hr    521 embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9    117 white prepupae new    188 white prepupae 12hr    754 white prepupae 24hr    1602 pupae 2d postWPP    408 pupae 3d postWPP    90 pupae 4d postWPP    150 adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    20 embryo 02-04hr    267 embryo 04-06hr    733 embryo 06-08hr    3058 embryo 08-10hr    3909 embryo 10-12hr    3343 embryo 12-14hr    1103 embryo 14-16hr    521 embryo 16-18hr    94 embryo 18-20hr    80 embryo 20-22hr    56 embryo 22-24hr    79 larva L1    31 larva L2    25 larva L3 12hr old    18 larva L3 puffstage 1-2    26 larva L3 puffstage 3-6    52 larva L3 puffstage 7-9    117 white prepupae new    188 white prepupae 12hr    754 white prepupae 24hr    1602 pupae 2d postWPP    408 pupae 3d postWPP    90 pupae 4d postWPP    150 adult male 01day    66 adult male 05day    52 adult male 30day    64 adult female 01day    66 adult female 05day    30 adult female 30day    28 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0261245    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0261245 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0261245 expression level Tissue   Expression Level Larval Central Nervous System    5.35 Larval Midgut    9.9 Larval Hindgut    5.9 Larval Malpighian Tubules no informative data Larval Fat Body    31.6 Larval Salivary Gland no informative data Larval Trachea    3.15 Larval Carcass    9.05 Adult Head    6.3 Adult Eye    5.5 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.3 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    11.5 Adult Malpighian Tubules    8.8 Adult Fat Body    9.1 Adult Salivary Gland no informative data Adult Heart    5.975 Adult VirginFemale Spermatheca    7.4 Adult InseminatedFemale Spermatheca    6.6 Adult Ovary    7.6 Adult Testis    7.7 Adult Male Accessory Gland no informative data Adult Carcass    7.2 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    5.35 Larval Midgut    9.9 Larval Hindgut    5.9 Larval Malpighian Tubules no informative data Larval Fat Body    31.6 Larval Salivary Gland no informative data Larval Trachea    3.15 Larval Carcass    9.05 Adult Head    6.3 Adult Eye    5.5 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.3 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    11.5 Adult Malpighian Tubules    8.8 Adult Fat Body    9.1 Adult Salivary Gland no informative data Adult Heart    5.975 Adult VirginFemale Spermatheca    7.4 Adult InseminatedFemale Spermatheca    6.6 Adult Ovary    7.6 Adult Testis    7.7 Adult Male Accessory Gland no informative data Adult Carcass    7.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    5.35 Larval Midgut    9.9 Larval Hindgut    5.9 Larval Malpighian Tubules no informative data Larval Fat Body    31.6 Larval Salivary Gland no informative data Larval Trachea    3.15 Larval Carcass    9.05 Adult Head    6.3 Adult Eye    5.5 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.3 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    11.5 Adult Malpighian Tubules    8.8 Adult Fat Body    9.1 Adult Salivary Gland no informative data Adult Heart    5.975 Adult VirginFemale Spermatheca    7.4 Adult InseminatedFemale Spermatheca    6.6 Adult Ovary    7.6 Adult Testis    7.7 Adult Male Accessory Gland no informative data Adult Carcass    7.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    5.35 Larval Midgut    9.9 Larval Hindgut    5.9 Larval Malpighian Tubules no informative data Larval Fat Body    31.6 Larval Salivary Gland no informative data Larval Trachea    3.15 Larval Carcass    9.05 Adult Head    6.3 Adult Eye    5.5 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.3 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    11.5 Adult Malpighian Tubules    8.8 Adult Fat Body    9.1 Adult Salivary Gland no informative data Adult Heart    5.975 Adult VirginFemale Spermatheca    7.4 Adult InseminatedFemale Spermatheca    6.6 Adult Ovary    7.6 Adult Testis    7.7 Adult Male Accessory Gland no informative data Adult Carcass    7.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0261245 expression level Tissue   Expression Level Larval Central Nervous System    5.35 Larval Midgut    9.9 Larval Hindgut    5.9 Larval Malpighian Tubules no informative data Larval Fat Body    31.6 Larval Salivary Gland no informative data Larval Trachea    3.15 Larval Carcass    9.05 Adult Head    6.3 Adult Eye    5.5 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.3 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    11.5 Adult Malpighian Tubules    8.8 Adult Fat Body    9.1 Adult Salivary Gland no informative data Adult Heart    5.975 Adult VirginFemale Spermatheca    7.4 Adult InseminatedFemale Spermatheca    6.6 Adult Ovary    7.6 Adult Testis    7.7 Adult Male Accessory Gland no informative data Adult Carcass    7.2 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    5.35 Larval Midgut    9.9 Larval Hindgut    5.9 Larval Malpighian Tubules no informative data Larval Fat Body    31.6 Larval Salivary Gland no informative data Larval Trachea    3.15 Larval Carcass    9.05 Adult Head    6.3 Adult Eye    5.5 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.3 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    11.5 Adult Malpighian Tubules    8.8 Adult Fat Body    9.1 Adult Salivary Gland no informative data Adult Heart    5.975 Adult VirginFemale Spermatheca    7.4 Adult InseminatedFemale Spermatheca    6.6 Adult Ovary    7.6 Adult Testis    7.7 Adult Male Accessory Gland no informative data Adult Carcass    7.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    5.35 Larval Midgut    9.9 Larval Hindgut    5.9 Larval Malpighian Tubules no informative data Larval Fat Body    31.6 Larval Salivary Gland no informative data Larval Trachea    3.15 Larval Carcass    9.05 Adult Head    6.3 Adult Eye    5.5 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.3 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    11.5 Adult Malpighian Tubules    8.8 Adult Fat Body    9.1 Adult Salivary Gland no informative data Adult Heart    5.975 Adult VirginFemale Spermatheca    7.4 Adult InseminatedFemale Spermatheca    6.6 Adult Ovary    7.6 Adult Testis    7.7 Adult Male Accessory Gland no informative data Adult Carcass    7.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    5.35 Larval Midgut    9.9 Larval Hindgut    5.9 Larval Malpighian Tubules no informative data Larval Fat Body    31.6 Larval Salivary Gland no informative data Larval Trachea    3.15 Larval Carcass    9.05 Adult Head    6.3 Adult Eye    5.5 Adult Brain    5.2 Adult Thoracic-Abdominal Ganglion    8.3 Adult Crop no informative data Adult Midgut no informative data Adult Hindgut    11.5 Adult Malpighian Tubules    8.8 Adult Fat Body    9.1 Adult Salivary Gland no informative data Adult Heart    5.975 Adult VirginFemale Spermatheca    7.4 Adult InseminatedFemale Spermatheca    6.6 Adult Ovary    7.6 Adult Testis    7.7 Adult Male Accessory Gland no informative data Adult Carcass    7.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules no informative data Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut no informative data Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    6.3   NA  Eye    5.5   NA  Brain    5.2   5.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    8.3   NA  Crop    no informative data   9.9  Midgut    no informative data   5.9  Hindgut    11.5   no informative data  Malpighian Tubules    8.8   31.6  Fat Body    9.1   no informative data  Salivary Gland    no informative data   NA  Heart    5.975   3.15  Trachea    NA   NA  VirginFemale Spermatheca    7.4   NA  InseminatedFemale Spermatheca    6.6   NA  Ovary    7.6   NA  Testis    7.7   NA  Male Accessory Gland    no informative data   9.05  Carcass    7.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0261245
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive sing22 sing23 sing27 viable, with Scer\GAL4pnr-MD237 singGD3396 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 singGD3396 Phenotype manifest in Allele A1-7 dorsal acute muscle 1 sing23 embryonic/larval somatic muscle singunspecified embryonic myoblast sing23 trichogen cell, with Scer\GAL4pnr-MD237 singGD3396
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of sing Class Mutagen Stocks Known lesion sing22 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes sing23 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes sing27 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes singunspecified 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of sing Class Mutagen Stocks Known lesion singGD3396 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes sing+t2.524 in vitro construct - genomic fragment 0 Yes singScer\UAS.cEa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UAS-sing.E} NA characterization construct P{sing+t2.524} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )
	There is no information supporting a molecular function term for this gene. (Based on a review of available data before: 2006)
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
myoblast fusion inferred from mutant phenotype (Maeland et al., 1998, Estrada et al., 2007)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
membrane inferred from electronic annotation with InterPro:IPR008253 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions sing allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261245
Orthologs
Genome-wide drosophilid orthologs	Dana\GF21837 Dere\GG19066 Dgri\GH12790 Dmoj\GI15231 Dper\GL13049 Dpse\GA11974 Dsec\GM13456 Dsim\GD17295 Dvir\GJ18690 Dwil\GK19928 Dyak\GE17611
Curated drosophilid orthologs	Dpse\GA11974   Dyak\GE17611
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL009493-PA Aedes aegypti (mosquito) AGAP000298-PA Anopheles gambiae (mosquito) XP_001122399.1 Apis mellifera (honey bee) CPIJ007151 Culex pipens (mosquito) XP_001606694.1 Nasonia vitripennis (wasp) PHUM003767-PA Pediculus humanus (human body louse) XP_972292.1 Tribolium castaneum (red flour beetle) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13567 NV18630 NV12336 LH18339 FBgn0068453 FBgn0221925 FBgn0205843 FBgn0188856 FBgn0168387 FBgn0072023 FBgn0150655 FBgn0137980 FBgn0120269 FBgn0111272 FBgn0098837 CPIJ007151 ACYPI53795 GB16083 AGAP000298 AAEL009493 PHUM000410 GLEAN_12091
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v12203 w1118; P{GD3396}v12203 v12202 w1118; P{GD3396}v12202/TM3
Genomic Clones ( 1 )
	BACR18G21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 23 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	FI15330 MIP13627
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	109170 DMG1-chrX.17.078.a EK263762 RP004114204 RP004143518 RP004169049 RP004171125 RP004187707 RP004197402 RP004199151 RP004210917 RP004212514 RP004218819 RP004230452 RP004250034 RP004254599 RP004413229 RP004487729 RP004494576 RP004520097 RP004553477
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261245 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32644 48094
Antibody Information
Other Information
Discoverer
Etymology
	The gene is named "singles bar" due to the mutant phenotype; single myoblasts accumulate within the embryo. (Estrada et al., 2007)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: singles-bar CG13011 (Estrada et al., 2007)
Additional comments
Other Comments
	sing is essential for myoblast fusion, being required for progression past the pre-fusion complex stage of myoblast fusion. (Estrada et al., 2007) Myoblasts fail to fuse to form the multinucleate muscles. (Maeland et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132918 RefSeq (Proteins) • NP_573146 DDBJ / EMBL / GenBank • AAF48643 Entrez Gene - A searchable database of RefSeq genes. • 32644 UniProtKB/TrEMBL • Q9VXD1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Marvel (IPR008253) MARVEL-like domain (IPR021128)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261245 DRSC - Results from RNAi screens. • FBgn0261245 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0261245 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261245 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32644 48094 InParanoid A subset of ortholog calls from InParanoid. • AAEL009493-PA Aedes aegypti (mosquito) AGAP000298-PA Anopheles gambiae (mosquito) XP_001122399.1 Apis mellifera (honey bee) CPIJ007151 Culex pipens (mosquito) XP_001606694.1 Nasonia vitripennis (wasp) PHUM003767-PA Pediculus humanus (human body louse) XP_972292.1 Tribolium castaneum (red flour beetle) modMine - Data generated by the modENCODE project. • FBgn0261245 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13567 NV18630 NV12336 LH18339 FBgn0068453 FBgn0221925 FBgn0205843 FBgn0188856 FBgn0168387 FBgn0072023 FBgn0150655 FBgn0137980 FBgn0120269 FBgn0111272 FBgn0098837 CPIJ007151 ACYPI53795 GB16083 AGAP000298 AAEL009493 PHUM000410 GLEAN_12091
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	CG13011 (Estrada et al., 2007) sing (Estrada et al., 2006, Estrada et al., 2007) singles-bar
Name Synonym	CG13011   singles bar (Maeland et al., 1996, Estrada et al., 2007)
Secondary FlyBase IDs
	FBgn0022723 FBgn0030771
References ( 10 )
Research paper	Estrada et al., 2007, Dev. Biol. 307(2): 328--339 The MARVEL domain protein, Singles Bar, is required for progression past the pre-fusion complex stage of myoblast fusion. [FBrf0200758] Ruiz-Gomez et al., 2000, Cell 102(2): 189--198 Drosophila dumbfounded: a myoblast attractant essential for fusion. [FBrf0130056]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Review	Paululat et al., 1999, Mech. Dev. 83(1,2): 17--26 Essential genes for myoblast fusion in Drosophila embryogenesis. [FBrf0108990]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Abstract	Estrada et al., 2006, A. Dros. Res. Conf. 47: 76 The MARVEL domain protein, SINGLES BAR, is required for progression past the pre-fusion complex stage of myoblast fusion. [FBrf0188985] Maeland et al., 1998, A. Dros. Res. Conf. 39: 676C Characterisation of singles bar, a gene required for myoblast fusion. [FBrf0101712] Maeland et al., 1996, Molec. Biol. Cell 7(Suppl.): 39a Characterisation of singles bar a new mutant in Drosophila melanogaster required for muscle development. [FBrf0092620]
FlyBase analysis	FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398]