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Gene Dmel\zuc

General Information
Symbol	Dmel\zuc	Species	D. melanogaster
Name	zucchini	Annotation symbol	CG12314
Feature type	protein_coding_gene	FlyBase ID	FBgn0261266
Gene Model Status	Current	Stock availability	9 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map	2-[45]
Cytogenetic map	33B5-33F2	Sequence location	2L:11,988,735..11,989,882 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene zucchini is referred to in FlyBase by the symbol Dmel\zuc (CG12314, FBgn0261266). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: piRNA metabolic process; gene silencing by RNA; oogenesis; dorsal appendage formation; karyosome formation. 13 alleles are reported. The phenotypes of these alleles are annotated with: egg; oocyte; karyosome; egg chamber; dorsal appendage. It has one annotated transcript and one annotated polypeptide. Protein features are: Phospholipase D-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1634161_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of zuc. Gene sequence location is 2L:11988735..11989861.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 33B5-33F2   Limits computationally determined from genome sequence between P{EP}MESK1EP980 and P{PZ}l(2)0181001810&P{EP}CG6785EP340; Left limit from inclusion within Df(2L)prd1.7 (FBrf0054123) Right limit from inclusion within Df(2L)Prl (FBrf0054123)
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	2-[45]
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\zuc for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.51
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) zuc-RA FBtr0080316  NM_135686   1148   253
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein zuc-PA   FBpp0079900   28.5   253   9.53     NP_609530   AAF53139
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Phospholipase D-like domain (IPR025202)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246582   AE014134 AAF53139   AW941588   AY118493 AAM49862   CL844459   CZ483815   EC228746
UniProtKB/Swiss-Prot	Q9VKD7
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   anterior endoderm anlage   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
mitochondrion inferred from direct assay (Huang et al., 2011) inferred from direct assay (assigned by UniProtKB) (Saito et al., 2010) P granule inferred from direct assay (Pane et al., 2007) NOT P granule inferred from direct assay (assigned by UniProtKB) (Saito et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    26.975 Larval Midgut    11.5 Larval Hindgut    12.1 Larval Malpighian Tubules    4.8 Larval Fat Body    8 Larval Salivary Gland    15.4 Larval Trachea    33.175 Larval Carcass    10.55 Adult Head    2.7 Adult Eye    0.85 Adult Brain    3.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    5.7 Adult Midgut    8.4 Adult Hindgut    3.8 Adult Malpighian Tubules    6.8 Adult Fat Body    8 Adult Salivary Gland    9.9 Adult Heart    1.9 Adult VirginFemale Spermatheca    5.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    97.6 Adult Testis    9.8 Adult Male Accessory Gland    20.8 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    26.975 Larval Midgut    11.5 Larval Hindgut    12.1 Larval Malpighian Tubules    4.8 Larval Fat Body    8 Larval Salivary Gland    15.4 Larval Trachea    33.175 Larval Carcass    10.55 Adult Head    2.7 Adult Eye    0.85 Adult Brain    3.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    5.7 Adult Midgut    8.4 Adult Hindgut    3.8 Adult Malpighian Tubules    6.8 Adult Fat Body    8 Adult Salivary Gland    9.9 Adult Heart    1.9 Adult VirginFemale Spermatheca    5.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    97.6 Adult Testis    9.8 Adult Male Accessory Gland    20.8 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    26.975 Larval Midgut    11.5 Larval Hindgut    12.1 Larval Malpighian Tubules    4.8 Larval Fat Body    8 Larval Salivary Gland    15.4 Larval Trachea    33.175 Larval Carcass    10.55 Adult Head    2.7 Adult Eye    0.85 Adult Brain    3.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    5.7 Adult Midgut    8.4 Adult Hindgut    3.8 Adult Malpighian Tubules    6.8 Adult Fat Body    8 Adult Salivary Gland    9.9 Adult Heart    1.9 Adult VirginFemale Spermatheca    5.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    97.6 Adult Testis    9.8 Adult Male Accessory Gland    20.8 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    26.975 Larval Midgut    11.5 Larval Hindgut    12.1 Larval Malpighian Tubules    4.8 Larval Fat Body    8 Larval Salivary Gland    15.4 Larval Trachea    33.175 Larval Carcass    10.55 Adult Head    2.7 Adult Eye    0.85 Adult Brain    3.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    5.7 Adult Midgut    8.4 Adult Hindgut    3.8 Adult Malpighian Tubules    6.8 Adult Fat Body    8 Adult Salivary Gland    9.9 Adult Heart    1.9 Adult VirginFemale Spermatheca    5.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    97.6 Adult Testis    9.8 Adult Male Accessory Gland    20.8 Adult Carcass    7.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    26.975 Larval Midgut    11.5 Larval Hindgut    12.1 Larval Malpighian Tubules    4.8 Larval Fat Body    8 Larval Salivary Gland    15.4 Larval Trachea    33.175 Larval Carcass    10.55 Adult Head    2.7 Adult Eye    0.85 Adult Brain    3.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    5.7 Adult Midgut    8.4 Adult Hindgut    3.8 Adult Malpighian Tubules    6.8 Adult Fat Body    8 Adult Salivary Gland    9.9 Adult Heart    1.9 Adult VirginFemale Spermatheca    5.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    97.6 Adult Testis    9.8 Adult Male Accessory Gland    20.8 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    26.975 Larval Midgut    11.5 Larval Hindgut    12.1 Larval Malpighian Tubules    4.8 Larval Fat Body    8 Larval Salivary Gland    15.4 Larval Trachea    33.175 Larval Carcass    10.55 Adult Head    2.7 Adult Eye    0.85 Adult Brain    3.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    5.7 Adult Midgut    8.4 Adult Hindgut    3.8 Adult Malpighian Tubules    6.8 Adult Fat Body    8 Adult Salivary Gland    9.9 Adult Heart    1.9 Adult VirginFemale Spermatheca    5.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    97.6 Adult Testis    9.8 Adult Male Accessory Gland    20.8 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    26.975 Larval Midgut    11.5 Larval Hindgut    12.1 Larval Malpighian Tubules    4.8 Larval Fat Body    8 Larval Salivary Gland    15.4 Larval Trachea    33.175 Larval Carcass    10.55 Adult Head    2.7 Adult Eye    0.85 Adult Brain    3.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    5.7 Adult Midgut    8.4 Adult Hindgut    3.8 Adult Malpighian Tubules    6.8 Adult Fat Body    8 Adult Salivary Gland    9.9 Adult Heart    1.9 Adult VirginFemale Spermatheca    5.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    97.6 Adult Testis    9.8 Adult Male Accessory Gland    20.8 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    26.975 Larval Midgut    11.5 Larval Hindgut    12.1 Larval Malpighian Tubules    4.8 Larval Fat Body    8 Larval Salivary Gland    15.4 Larval Trachea    33.175 Larval Carcass    10.55 Adult Head    2.7 Adult Eye    0.85 Adult Brain    3.4 Adult Thoracic-Abdominal Ganglion    2.8 Adult Crop    5.7 Adult Midgut    8.4 Adult Hindgut    3.8 Adult Malpighian Tubules    6.8 Adult Fat Body    8 Adult Salivary Gland    9.9 Adult Heart    1.9 Adult VirginFemale Spermatheca    5.9 Adult InseminatedFemale Spermatheca    4.9 Adult Ovary    97.6 Adult Testis    9.8 Adult Male Accessory Gland    20.8 Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2.7   NA  Eye    0.85   NA  Brain    3.4   26.975  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.8   NA  Crop    5.7   no informative data  Midgut    8.4   12.1  Hindgut    3.8   4.8  Malpighian Tubules    6.8   8  Fat Body    8   no informative data  Salivary Gland    9.9   NA  Heart    1.9   33.175  Trachea    NA   NA  VirginFemale Spermatheca    5.9   NA  InseminatedFemale Spermatheca    4.9   NA  Ovary    97.6   NA  Testis    9.8   NA  Male Accessory Gland    no informative data   no informative data  Carcass    7.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    12 ovary, mated 4-day female    8 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    12 ovary, mated 4-day female    8 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    12 ovary, mated 4-day female    8 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    12 ovary, mated 4-day female    8 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    12 ovary, mated 4-day female    8 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    12 ovary, mated 4-day female    8 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    12 ovary, mated 4-day female    8 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    1 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    1 ovary, virgin 4-day female    12 ovary, mated 4-day female    8 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    39 embryo 04-06hr    27 embryo 06-08hr    15 embryo 08-10hr    22 embryo 10-12hr    15 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    7 embryo 18-20hr    3 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    11 adult female 05day    14 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (28) embryo 02-04hr  (39) embryo 04-06hr  (27) embryo 06-08hr    15 embryo 08-10hr    22 embryo 10-12hr    15 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    7 embryo 18-20hr    3 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    11 adult female 05day    14 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    39 embryo 04-06hr    27 embryo 06-08hr    15 embryo 08-10hr    22 embryo 10-12hr    15 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    7 embryo 18-20hr    3 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    11 adult female 05day    14 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    39 embryo 04-06hr    27 embryo 06-08hr    15 embryo 08-10hr    22 embryo 10-12hr    15 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    7 embryo 18-20hr    3 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    11 adult female 05day    14 adult female 30day    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    39 embryo 04-06hr    27 embryo 06-08hr    15 embryo 08-10hr    22 embryo 10-12hr    15 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    7 embryo 18-20hr    3 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    11 adult female 05day    14 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  28 embryo 02-04hr  (39) embryo 04-06hr  27 embryo 06-08hr    15 embryo 08-10hr    22 embryo 10-12hr    15 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    7 embryo 18-20hr    3 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    11 adult female 05day    14 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    39 embryo 04-06hr    27 embryo 06-08hr    15 embryo 08-10hr    22 embryo 10-12hr    15 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    7 embryo 18-20hr    3 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    11 adult female 05day    14 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    28 embryo 02-04hr    39 embryo 04-06hr    27 embryo 06-08hr    15 embryo 08-10hr    22 embryo 10-12hr    15 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    7 embryo 18-20hr    3 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    2 white prepupae 24hr    2 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    2 adult male 01day    1 adult male 05day    2 adult male 30day    1 adult female 01day    11 adult female 05day    14 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    6 embryonic 1182-4H    4 embryonic GM2    4 embryonic Kc167    8 embryonic S1    5 embryonic S3    6 leg disc CME L1    8 wing disc CME-W2    5 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    9 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    4 ovary fGS/OSS    10 ovary OSC    8 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    6 embryonic 1182-4H    4 embryonic GM2    4 embryonic Kc167    8 embryonic S1    5 embryonic S3    6 leg disc CME L1    8 wing disc CME-W2    5 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    9 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    4 ovary fGS/OSS    10 ovary OSC    8 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    6 embryonic 1182-4H    4 embryonic GM2    4 embryonic Kc167    8 embryonic S1    5 embryonic S3    6 leg disc CME L1    8 wing disc CME-W2    5 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    9 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    4 ovary fGS/OSS    10 ovary OSC    8 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    6 embryonic 1182-4H    4 embryonic GM2    4 embryonic Kc167    8 embryonic S1    5 embryonic S3    6 leg disc CME L1    8 wing disc CME-W2    5 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    9 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    4 ovary fGS/OSS    10 ovary OSC    8 ovary OSS    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    6 embryonic 1182-4H    4 embryonic GM2    4 embryonic Kc167    8 embryonic S1    5 embryonic S3    6 leg disc CME L1    8 wing disc CME-W2    5 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    9 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    4 ovary fGS/OSS    10 ovary OSC    8 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    6 embryonic 1182-4H    4 embryonic GM2    4 embryonic Kc167    8 embryonic S1    5 embryonic S3    6 leg disc CME L1    8 wing disc CME-W2    5 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    9 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    4 ovary fGS/OSS    10 ovary OSC    8 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    6 embryonic 1182-4H    4 embryonic GM2    4 embryonic Kc167    8 embryonic S1    5 embryonic S3    6 leg disc CME L1    8 wing disc CME-W2    5 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    9 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    4 ovary fGS/OSS    10 ovary OSC    8 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    6 embryonic 1182-4H    4 embryonic GM2    4 embryonic Kc167    8 embryonic S1    5 embryonic S3    6 leg disc CME L1    8 wing disc CME-W2    5 wing disc ML-DmD8    9 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    9 wing disc ML-DmD32    3 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    4 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    1 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    4 ovary fGS/OSS    10 ovary OSC    8 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    3 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • LD05365 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0261266
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable zucEY11457 zucf01342 Sterility Allele female sterile zucHM27 zucRS49 zucSG63 fertile zucEY11457 zucf01342 Phenotype manifest in Allele dorsal appendage zucRS49 dorsal appendage (with Df(2L)Prl) zucHM27 zucSG63 egg zucRS49 egg (with Df(2L)Prl) zucHM27 zucSG63 egg chamber zucunspecified karyosome zucunspecified oocyte zucunspecified
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of zuc Class Mutagen Stocks Known lesion zucf01342 piggyBac transposase 2 -- zucRS49 ethyl methanesulfonate 2 Yes zucEY11457 P-element activity 1 -- zucHM27 ethyl methanesulfonate 0 Yes zucSG63 ethyl methanesulfonate 0 Yes zucunspecified 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of zuc Class Mutagen Stocks Known lesion zucGD6746 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes zucGL00111 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes zucGL00112 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes zucHMS03002 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes zucKK101658 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes zucScer\UAS.P\T.T:Avic\GFP-EGFP 0 Yes zucScer\UAS.P\T.T:Ivir\HA1 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6031 (Ryder, 2004.4.26) Df(2L)prd1.7 (Schupbach and Wieschaus, 1991) Df(2L)Prl (Schupbach and Wieschaus, 1991)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK101658} NA P{TRiP.GL00111} NA P{TRiP.GL00112} NA P{TRiP.HMS03002} NA P{UASp-EGFP-zuc} NA P{UASp-HA-zuc} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}zucf01342 NA insertion of mobile activating element P{EPgy2}zucEY11457 NA insertion of enhancer trap P{lacW}l(2)SH2136SH2136 No
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
dorsal appendage formation inferred from mutant phenotype (Schupbach and Wieschaus, 1991) gene silencing by RNA inferred from mutant phenotype (Pane et al., 2007) karyosome formation inferred from mutant phenotype (Ghabrial et al., 1998) oogenesis inferred from mutant phenotype (Schupbach and Wieschaus, 1991) piRNA metabolic process inferred from mutant phenotype (assigned by UniProtKB) (Saito et al., 2010)
Cellular Component
CV term Evidence References
mitochondrion inferred from direct assay (Huang et al., 2011) inferred from direct assay (assigned by UniProtKB) (Saito et al., 2010) P granule inferred from direct assay (Pane et al., 2007) NOT P granule inferred from direct assay (assigned by UniProtKB) (Saito et al., 2010)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
oogenesis traceable author statement (van Eeden and St. Johnston, 1999)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions zuc allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261266
Orthologs
OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6RJGMV Dipteran inclusive ortholog search EOG69W2QD Insect inclusive ortholog search EOG6FXQ4S Arthropod inclusive ortholog search EOG6P8D17 Metazoa inclusive ortholog search EOG6ZKXFV
Orthologs in Drosophila Species (EOG6RJGMV)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\zuc     Drosophila simulans   Dsim\GD22146 Y   Drosophila sechellia   Dsec\GM26473 Y   Drosophila erecta   Dere\GG10288 Y   Drosophila yakuba   Dyak\GE12577 Y   Drosophila ananassae   Dana\GF14755 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11550 Y   Drosophila persimilis   Dper\GL19579 Y   Drosophila willistoni   Dwil\GK19043 Y   Drosophila virilis   Dvir\GJ16996     Drosophila mojavensis   Dmoj\GI13482     Drosophila mojavensis   Dmoj\GI21822     Drosophila mojavensis   Dmoj\GI24814 Y   Drosophila grimshawi   Dgri\GH20648     Drosophila grimshawi   Dgri\GH13819 Y
Orthologs in non-Drosophila Dipterans (EOG69W2QD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011385
Orthologs in non-Dipteran Insects (EOG6FXQ4S)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB41641   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004552   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19970   Atta cephalotes Leafcutter ant  Acep\ACEP24675   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18504   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL10887   Linepithema humile Argentine ant  Lhum\LH20516   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16156   Solenopsis invicta Red fire ant  Sinv\SINV24158   Acyrthosiphon pisum Pea aphid  Apim\ACYPI47236   Bombyx mori Silkmoth  Bmor\BGIBMGA007918   Pediculus humanus Human body louse  Phum\PHUM318600   Tribolium castaneum Red flour beetle  Tcas\TC010319   Tribolium castaneum Red flour beetle  Tcas\TC000031
Orthologs in non-Insect Arthropods (EOG6P8D17)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_308957   Daphnia pulex Water flea  Dpux\ARRDC3   Daphnia pulex Water flea  Dpux\JGI_V11_331332   Ixodes scapularis Deer tick  Isca\ISCW009492
Orthologs in non-Arthropod Metazoa (EOG6ZKXFV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012787gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021981gn   Danio rerio Zebrafish  Drer\pld6   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000005747   Mus musculus House mouse  Mmus\Pld6   Rattus norvegicus Norway rat  Rnor\Pld6   Homo sapiens Human  Hsap\PLD6
Human Orthologs (1)
Gene OMIM HGNC Hsap\PLD6
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22146 Drosophila sechellia Dsec\GM26473 Drosophila erecta Dere\GG10288 Drosophila yakuba Dyak\GE12577 Drosophila ananassae Dana\GF14755 Drosophila pseudoobscura pseudoobscura Dpse\GA11550 Drosophila persimilis Dper\GL19579 Drosophila willistoni Dwil\GK19043 Drosophila virilis Dvir\GJ22526 Drosophila mojavensis Dmoj\GI24814 Drosophila grimshawi Dgri\GH13819
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	18427 w1118; PBac{WH}zucf01342 20310 y1 w67c23; P{EPgy2}zucEY11457 35227 y1 sc* v1; P{TRiP.GL00111}attP2 35228 y1 sc* v1; P{TRiP.GL00112}attP2 36742 y1 sc* v1; P{TRiP.HMS03002}attP40 5120 zucRS49 cn1 bw1/CyO, l(2)DTS5131
Harvard	- PBac{WH}zucf01342
Kyoto	108052 zucRS49 cn1 bw1/CyO, l(2)DTS5131
VDRC	v48764 w1118; P{GD6746}v48764/TM3
Genomic Clones ( 1 )
	BACR37P12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 2 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD05365
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	CK01.1B.F8
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261266 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34609
Antibody Information
	polyclonal (Haase et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: zuc CG12314 (Pane et al., 2007)
Additional comments
Other Comments
	zuc is required for rasiRNA production in the female germline. (Pane et al., 2007) Mutations at the zuc locus cause defects in midoogenesis. (Schupbach and Wieschaus, 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135686 RefSeq (Proteins) • NP_609530 DDBJ / EMBL / GenBank • AA246582 AAF53139 AAM49862 AW941588 AY118493 CL844459 CZ483815 Entrez Gene - A searchable database of RefSeq genes. • 34609 UniProtKB/Swiss-Prot • Q9VKD7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Phospholipase D-like domain (IPR025202) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD05365
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261266 DRSC - Results from RNAi screens. • FBgn0261266 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0261266 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261266 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34609 modMine - Data generated by the modENCODE project. • FBgn0261266
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG12314 (Pane et al., 2007, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) ZUC (Kolaczkowski et al., 2011) zuc (Malone et al., 2009, Pane et al., 2007, Pane et al., 2007, Pane et al., 2007, Chambeyron et al., 2008, Robine et al., 2009, Saito et al., 2009, Todeschini et al., 2010, Olivieri et al., 2010, Haase et al., 2010, Nagao et al., 2010, Saito et al., 2010, Handler et al., 2011, Klenov et al., 2011, Olivieri et al., 2012) Zuc (Huang et al., 2011)
Name Synonym	CG12314   zucchini (Castillo et al., 2011, Malone et al., 2009, Pane et al., 2007, Pane et al., 2007, Pane et al., 2007, Robine et al., 2009, Saito et al., 2009, Todeschini et al., 2010, Olivieri et al., 2010, Haase et al., 2010, Handler et al., 2011)
Secondary FlyBase IDs
	FBgn0004056 FBgn0032389
References ( 38 )
Generate a list of	All references relating to this gene ( 38 )
List References by type	Research paper  ( 21 ) Supplementary material  ( 2 ) Review  ( 3 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 3 ) Website  ( 1 )
Recent research papers ( 8 )
	Lee and Langley, 2012, Genetics 192(4): 1411--1432 Long-term and short-term evolutionary impacts of transposable elements on Drosophila. [FBrf0220133] Olivieri et al., 2012, Mol. Cell 47(6): 954--969 The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila. [FBrf0219572] Voigt et al., 2012, RNA 18(12): 2128--2134 Crystal structure of the primary piRNA biogenesis factor Zucchini reveals similarity to the bacterial PLD endonuclease Nuc. [FBrf0220011] Castillo et al., 2011, BMC Evol. Biol. 11: 258 Molecular evolution under increasing transposable element burden in Drosophila: A speed limit on the evolutionary arms race. [FBrf0216257] Handler et al., 2011, EMBO J. 30(19): 3977--3993 A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors. [FBrf0216344] Huang et al., 2011, Dev. Cell 20(3): 376--387 piRNA-Associated Germline Nuage Formation and Spermatogenesis Require MitoPLD Profusogenic Mitochondrial-Surface Lipid Signaling. [FBrf0213221] Klenov et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(46): 18760--18765 Separation of stem cell maintenance and transposon silencing functions of Piwi protein. [FBrf0216776] Kolaczkowski et al., 2011, Mol. Biol. Evol. 28(2): 1033--1042 Recurrent adaptation in RNA interference genes across the Drosophila phylogeny. [FBrf0212798] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...