• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Cul-3

General Information
Symbol	Dmel\Cul-3	Species	D. melanogaster
Name	Cullin-3	Annotation symbol	CG42616
Feature type	protein_coding_gene	FlyBase ID	FBgn0261268
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	cul3, gft, l(2)br34, CG11861, l(2)35Cd, dCul3, BG:DS07851.2, br34, CG31829
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	35C5-35C5	Sequence location	2L:15,265,245..15,272,011 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Cullin-3 is referred to in FlyBase by the symbol Dmel\Cul-3 (CG42616, FBgn0261268). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: ubiquitin-protein ligase activity. There is experimental evidence that it is involved in the biological process: activation of caspase activity; sensory organ development; dendrite morphogenesis; sperm individualization; imaginal disc-derived wing morphogenesis; axonogenesis; protein ubiquitination. 33 alleles are reported. The phenotypes of these alleles are annotated with: sensillum campaniformium; wing margin bristle; anterior crossvein; wing vein L4; spermatid; wing hair. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Cullin homology; Cullin protein, neddylation domain; Cullin repeat-like-containing domain; Cullin, N-terminal; Cullin, conserved site; Winged helix-turn-helix transcription repressor DNA-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. Gene sequence location is 2L:15265245..15272011.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	l(2)35Cd l(2)35Cd2, but not other alleles, fails to complement In(2LR)Scorv1.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features BKN27226 BKN20070 HFA03537 References Ayyub, 2011, J. Genet. 90(2): 239--249 Controlled Vocabulary Terms testis male germline cell adult stage Clones 40526 53030 40590 EK213254 EK153615 EK170319 EK105808 82182
FB2011_10	References Du et al., 2011, PLoS ONE 6(9): e24168 Controlled Vocabulary Terms day 6 of adulthood day 5 of adulthood male day 7 of adulthood spermatozoon
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 35C5-35C5   Limits computationally determined from genome sequence between P{EP}vigEP812&P{PZ}stc05441 and P{EP}esgEP633&P{PZ}esg07082
Experimentally Determined Cytological Location
Cytogenetic map Notes References 35D1-35D4   (determined by in situ hybridisation)   (Ashburner et al., 1999) 35D1-35D4   (determined by in situ hybridisation)   (Spradling et al., 1999) 35D1-35D4   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes	Recombinants of gft06430 indicate that the gene order is rd l(2)35Cc gft. (Ashburner et al., 1999.10.12)
Gene Model & Products
Please see the GBrowse view of Dmel\Cul-3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene merge based on EST/cDNA data. Gene model reviewed during 5.43
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cul-3-RC FBtr0302157  NM_078849   3010   773 Cul-3-RD FBtr0302156  NM_165111   4319   773 Cul-3-RE FBtr0302158  NM_165110   3272   773 Cul-3-RF FBtr0302159  NM_165112   3558   934
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Cul-3-PC   FBpp0291367   89.6   773   9.09     NP_523573   AAF53450 Cul-3-PD   FBpp0291366   89.6   773   9.09     NP_723908   AAN10895 Cul-3-PE   FBpp0291368   89.6   773   9.09     NP_723907   AAF53451 Cul-3-PF   FBpp0291369   106.9   934   9.51     NP_723909   AAN10896
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Cullin, N-terminal (IPR001373) Winged helix-turn-helix transcription repressor DNA-binding (IPR011991) Cullin, conserved site (IPR016157) Cullin homology (IPR016158) Cullin repeat-like-containing domain (IPR016159) Cullin protein, neddylation domain (IPR019559)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202900   AA391453   AA816692   AA949500   AA979495   AE014134 AAF53450   AE014134 AAF53451   AE014134 AAN10895   AE014134 AAN10896   AE003412 AAF44933   AI109574   AI135442   AI135455   AI516836   AI519731   AI542433   AI944606   AI945458   AI946599   AW942138   BE662122   BE662150   BE977749   BF485814   BF486537   BF486693   BF487211   BF489462   BF491675   BF492504   BF492505   BF493250   BF493908   BF494546   BF494984   BF496566   BF498042   BF498756   BF501064   BF503498   BF503822   BF505539   BF506110   BF506272   BI168581   BI169489   BI230380   BI361974   BI364250   BI365833   BI367290   BI370606   BI370620   BI637884   BT021406 AAX33554   BT082116   BT099635   CA806698   CK135168   CL868471   CO181133   CO185657   CO193027   CO272077   CO274144   CO275958   CO280282   CO280882   CO285049   CO292753   CO296265   CO297339   CO297670   CO297765   CO297792   CO300962   CO309726   CO317093   CO318464   CO320572   CZ479946   EC061888   EC064382   EC064462   EC066587   EC072051   EC074125   EC077753   EC078305   EC078731   EC084843   EC087428   EC199367   EC200021   EC208920   EC215628   EC217376   EC222806   EC238333   EC246017   EC250474   G00415   GH739582   GH740345   GH741357   GH741420   GH742279   GH742455   GH743213   GH744145   GH745467   GH746358   GH746717   GH761564   GH761967   GH762578   GH763206   GH763381   GH763579   GH765704   GH766571   GH766759   GH766796   GH767613   GH767698   GH768630   GH769698   L80117
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V475 Q8IP45
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   eye disc | ubiquitous   (Ou et al., 2002) RT-PCR Stage Tissue/Position (including subcellular localization) Reference adult stage | male   testis   (Arama et al., 2007) Comment:male-specific isoform male germline cell   (Arama et al., 2007) Comment:male-specific isoform
Additional Descriptive Data
	One Cul-3 isoform (with the downstream translation start) is detected only in male germline cells. The other isoform is detected in somatic cells. (Arama et al., 2007)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0261268    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0261268 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0261268 expression level Developmental Stage   Expression Level embryo 00-02hr    2304 embryo 02-04hr    2135 embryo 04-06hr    3206 embryo 06-08hr    2809 embryo 08-10hr    2581 embryo 10-12hr    1808 embryo 12-14hr    2241 embryo 14-16hr    1201 embryo 16-18hr    1096 embryo 18-20hr    824 embryo 20-22hr    761 embryo 22-24hr    950 larva L1    735 larva L2    993 larva L3 12hr old    818 larva L3 puffstage 1-2    1140 larva L3 puffstage 3-6    2118 larva L3 puffstage 7-9    3829 white prepupae new    3872 white prepupae 12hr    3236 white prepupae 24hr    4021 pupae 2d postWPP    4230 pupae 3d postWPP    3733 pupae 4d postWPP    2841 adult male 01day    5668 adult male 05day    6500 adult male 30day    5572 adult female 01day    1219 adult female 05day    2146 adult female 30day    1948 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2304) embryo 02-04hr  (2135) embryo 04-06hr  (3206) embryo 06-08hr  (2809) embryo 08-10hr  (2581) embryo 10-12hr  (1808) embryo 12-14hr  (2241) embryo 14-16hr  (1201) embryo 16-18hr  (1096) embryo 18-20hr  (824) embryo 20-22hr  (761) embryo 22-24hr  (950) larva L1  (735) larva L2  (993) larva L3 12hr old  (818) larva L3 puffstage 1-2  (1140) larva L3 puffstage 3-6  (2118) larva L3 puffstage 7-9  (3829) white prepupae new  (3872) white prepupae 12hr  (3236) white prepupae 24hr  (4021) pupae 2d postWPP  (4230) pupae 3d postWPP  (3733) pupae 4d postWPP  (2841) adult male 01day  (5668) adult male 05day  (6500) adult male 30day  (5572) adult female 01day  (1219) adult female 05day  (2146) adult female 30day  (1948) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2304) embryo 02-04hr  (2135) embryo 04-06hr  (3206) embryo 06-08hr  (2809) embryo 08-10hr  (2581) embryo 10-12hr  (1808) embryo 12-14hr  (2241) embryo 14-16hr    1201 embryo 16-18hr    1096 embryo 18-20hr    824 embryo 20-22hr    761 embryo 22-24hr    950 larva L1    735 larva L2    993 larva L3 12hr old    818 larva L3 puffstage 1-2    1140 larva L3 puffstage 3-6  (2118) larva L3 puffstage 7-9  (3829) white prepupae new  (3872) white prepupae 12hr  (3236) white prepupae 24hr  (4021) pupae 2d postWPP  (4230) pupae 3d postWPP  (3733) pupae 4d postWPP  (2841) adult male 01day  (5668) adult male 05day  (6500) adult male 30day  (5572) adult female 01day    1219 adult female 05day  (2146) adult female 30day  (1948) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2304 embryo 02-04hr    2135 embryo 04-06hr    3206 embryo 06-08hr    2809 embryo 08-10hr    2581 embryo 10-12hr    1808 embryo 12-14hr    2241 embryo 14-16hr    1201 embryo 16-18hr    1096 embryo 18-20hr    824 embryo 20-22hr    761 embryo 22-24hr    950 larva L1    735 larva L2    993 larva L3 12hr old    818 larva L3 puffstage 1-2    1140 larva L3 puffstage 3-6    2118 larva L3 puffstage 7-9    3829 white prepupae new    3872 white prepupae 12hr    3236 white prepupae 24hr    4021 pupae 2d postWPP    4230 pupae 3d postWPP    3733 pupae 4d postWPP    2841 adult male 01day    5668 adult male 05day    6500 adult male 30day    5572 adult female 01day    1219 adult female 05day    2146 adult female 30day    1948 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2304 embryo 02-04hr    2135 embryo 04-06hr    3206 embryo 06-08hr    2809 embryo 08-10hr    2581 embryo 10-12hr    1808 embryo 12-14hr    2241 embryo 14-16hr    1201 embryo 16-18hr    1096 embryo 18-20hr    824 embryo 20-22hr    761 embryo 22-24hr    950 larva L1    735 larva L2    993 larva L3 12hr old    818 larva L3 puffstage 1-2    1140 larva L3 puffstage 3-6    2118 larva L3 puffstage 7-9    3829 white prepupae new    3872 white prepupae 12hr    3236 white prepupae 24hr    4021 pupae 2d postWPP    4230 pupae 3d postWPP    3733 pupae 4d postWPP    2841 adult male 01day    5668 adult male 05day    6500 adult male 30day    5572 adult female 01day    1219 adult female 05day    2146 adult female 30day    1948 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0261268 expression level Developmental Stage   Expression Level embryo 00-02hr    2304 embryo 02-04hr    2135 embryo 04-06hr    3206 embryo 06-08hr    2809 embryo 08-10hr    2581 embryo 10-12hr    1808 embryo 12-14hr    2241 embryo 14-16hr    1201 embryo 16-18hr    1096 embryo 18-20hr    824 embryo 20-22hr    761 embryo 22-24hr    950 larva L1    735 larva L2    993 larva L3 12hr old    818 larva L3 puffstage 1-2    1140 larva L3 puffstage 3-6    2118 larva L3 puffstage 7-9    3829 white prepupae new    3872 white prepupae 12hr    3236 white prepupae 24hr    4021 pupae 2d postWPP    4230 pupae 3d postWPP    3733 pupae 4d postWPP    2841 adult male 01day    5668 adult male 05day    6500 adult male 30day    5572 adult female 01day    1219 adult female 05day    2146 adult female 30day    1948 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2304) embryo 02-04hr  (2135) embryo 04-06hr  (3206) embryo 06-08hr  (2809) embryo 08-10hr  (2581) embryo 10-12hr  (1808) embryo 12-14hr  (2241) embryo 14-16hr  (1201) embryo 16-18hr  (1096) embryo 18-20hr  (824) embryo 20-22hr  (761) embryo 22-24hr  (950) larva L1  (735) larva L2  (993) larva L3 12hr old  (818) larva L3 puffstage 1-2  (1140) larva L3 puffstage 3-6  (2118) larva L3 puffstage 7-9  (3829) white prepupae new  (3872) white prepupae 12hr  (3236) white prepupae 24hr  (4021) pupae 2d postWPP  (4230) pupae 3d postWPP  (3733) pupae 4d postWPP  (2841) adult male 01day  (5668) adult male 05day  (6500) adult male 30day  (5572) adult female 01day  (1219) adult female 05day  (2146) adult female 30day  (1948) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  2304 embryo 02-04hr  2135 embryo 04-06hr  (3206) embryo 06-08hr  (2809) embryo 08-10hr  (2581) embryo 10-12hr  1808 embryo 12-14hr  2241 embryo 14-16hr    1201 embryo 16-18hr    1096 embryo 18-20hr    824 embryo 20-22hr    761 embryo 22-24hr    950 larva L1    735 larva L2    993 larva L3 12hr old    818 larva L3 puffstage 1-2    1140 larva L3 puffstage 3-6  2118 larva L3 puffstage 7-9  (3829) white prepupae new  (3872) white prepupae 12hr  (3236) white prepupae 24hr  (4021) pupae 2d postWPP  (4230) pupae 3d postWPP  (3733) pupae 4d postWPP  (2841) adult male 01day  (5668) adult male 05day  (6500) adult male 30day  (5572) adult female 01day    1219 adult female 05day  2146 adult female 30day  1948 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2304 embryo 02-04hr    2135 embryo 04-06hr    3206 embryo 06-08hr    2809 embryo 08-10hr    2581 embryo 10-12hr    1808 embryo 12-14hr    2241 embryo 14-16hr    1201 embryo 16-18hr    1096 embryo 18-20hr    824 embryo 20-22hr    761 embryo 22-24hr    950 larva L1    735 larva L2    993 larva L3 12hr old    818 larva L3 puffstage 1-2    1140 larva L3 puffstage 3-6    2118 larva L3 puffstage 7-9    3829 white prepupae new    3872 white prepupae 12hr    3236 white prepupae 24hr    4021 pupae 2d postWPP    4230 pupae 3d postWPP    3733 pupae 4d postWPP    2841 adult male 01day    5668 adult male 05day    6500 adult male 30day    5572 adult female 01day    1219 adult female 05day    2146 adult female 30day    1948 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2304 embryo 02-04hr    2135 embryo 04-06hr    3206 embryo 06-08hr    2809 embryo 08-10hr    2581 embryo 10-12hr    1808 embryo 12-14hr    2241 embryo 14-16hr    1201 embryo 16-18hr    1096 embryo 18-20hr    824 embryo 20-22hr    761 embryo 22-24hr    950 larva L1    735 larva L2    993 larva L3 12hr old    818 larva L3 puffstage 1-2    1140 larva L3 puffstage 3-6    2118 larva L3 puffstage 7-9    3829 white prepupae new    3872 white prepupae 12hr    3236 white prepupae 24hr    4021 pupae 2d postWPP    4230 pupae 3d postWPP    3733 pupae 4d postWPP    2841 adult male 01day    5668 adult male 05day    6500 adult male 30day    5572 adult female 01day    1219 adult female 05day    2146 adult female 30day    1948 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0261268    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0261268 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0261268
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 Cul-3GD7408 lethal | embryonic stage | recessive Cul-3d577 Cul-3gft1 Cul-3gft2 Cul-3gft3 Cul-3gft4 Cul-3gft5 Cul-3gft6 lethal | germline clone | maternal effect | recessive Cul-306430 Cul-3gft2 lethal | larval stage (with Df(2L)TE35D-15) Cul-306430 Cul-3d577 Cul-3gft1 Cul-3gft2 lethal | larval stage | recessive Cul-306430 Cul-3d577 Cul-3gft1 Cul-3gft2 Cul-3gft4 lethal | recessive Cul-306430 Cul-3gft2 viable, with Scer\GAL4pnr-MD237 Cul-3GD10381 Sterility Allele male fertile (with Cul-306430) Cul-3mds1 Cul-3mds2 Cul-3mds3 Cul-3mds4 Cul-3mds5 male fertile (with Cul-3gft3) Cul-3mds3 Cul-3mds4 Cul-3mds5 male fertile (with Cul-3mds1) Cul-306430 male fertile (with Cul-3mds2) Cul-306430 male fertile (with Cul-3mds3) Cul-306430 Cul-3gft3 male fertile (with Cul-3mds4) Cul-306430 Cul-3gft3 male fertile (with Cul-3mds5) Cul-306430 Cul-3gft3 male sterile Cul-3mds1 Cul-3mds2 Cul-3mds3 Cul-3mds4 Cul-3mds5 male sterile (with Cul-3d577) Cul-3mds1 Cul-3mds2 Cul-3mds3 Cul-3mds4 Cul-3mds5 male sterile (with Cul-3gft1) Cul-3mds1 Cul-3mds2 Cul-3mds3 Cul-3mds4 Cul-3mds5 male sterile (with Cul-3gft2) Cul-3mds1 Cul-3mds2 Cul-3mds3 Cul-3mds4 Cul-3mds5 male sterile (with Cul-3gft3) Cul-3mds1 Cul-3mds2 male sterile (with Cul-3gft4) Cul-3mds1 Cul-3mds2 Cul-3mds4 male sterile (with Cul-3GR18) Cul-3mds1 Cul-3mds2 Cul-3mds3 Cul-3mds4 Cul-3mds5 male sterile (with Cul-3mds1) Cul-3d577 Cul-3gft1 Cul-3gft2 Cul-3gft3 Cul-3gft4 Cul-3GR18 male sterile (with Cul-3mds2) Cul-3d577 Cul-3gft1 Cul-3gft2 Cul-3gft3 Cul-3gft4 Cul-3GR18 male sterile (with Cul-3mds3) Cul-3d577 Cul-3gft1 Cul-3gft2 Cul-3GR18 male sterile (with Cul-3mds4) Cul-3d577 Cul-3gft1 Cul-3gft2 Cul-3gft4 Cul-3GR18 male sterile (with Cul-3mds5) Cul-3d577 Cul-3gft1 Cul-3gft2 Cul-3GR18 male sterile | partially (with Cul-3gft4) Cul-3mds3 Cul-3mds5 male sterile | partially (with Cul-3mds3) Cul-3gft4 male sterile | partially (with Cul-3mds5) Cul-3gft4 Other Phenotypes Allele neuroanatomy defective Cul-3gft2 Cul-3gft8 visible, with Scer\GAL4dpp.blk1 Cul-3Scer\UAS.cMa visible, with Scer\GAL4sca-4512 Cul-3Scer\UAS.cMa visible | recessive | somatic clone Cul-306430 Cul-3gft2 Phenotype manifest in Allele anterior crossvein, with Scer\GAL4dpp.blk1 Cul-3Scer\UAS.cMa dorsal appendage | germline clone | maternal effect Cul-306430 Cul-3gft2 egg | germline clone | maternal effect Cul-306430 Cul-3gft2 external sensory organ | ectopic | somatic clone Cul-306430 Cul-3gft2 macrochaeta | somatic clone Cul-306430 Cul-3gft2 mesothoracic tergum & macrochaeta, with Scer\GAL4sca-4512 Cul-3Scer\UAS.cMa microchaeta | somatic clone Cul-306430 Cul-3gft2 neuron & alpha-lobe Cul-3gft8 neuron & beta-lobe Cul-3gft8 neuron & gamma-lobe Cul-3gft8 neuron & mushroom body Cul-3gft2 Cul-3gft8 scutellum & macrochaeta, with Scer\GAL4sca-4512 Cul-3Scer\UAS.cMa sensillum campaniformium, with Scer\GAL4dpp.blk1 Cul-3Scer\UAS.cMa sensillum campaniformium & wing, with Scer\GAL4sca-4512 Cul-3Scer\UAS.cMa sensillum campaniformium | ectopic | somatic clone Cul-306430 Cul-3gft2 sensillum campaniformium of dorsal radius | ectopic | somatic clone Cul-306430 Cul-3gft2 sensory mother cell | cell autonomous | ectopic | somatic clone Cul-306430 Cul-3gft2 spermatid Cul-3mds5 spermatid (with Cul-3gft1) Cul-3mds1 spermatid (with Cul-3gft2) Cul-3mds1 spermatid (with Cul-3gft4) Cul-3mds1 spermatid (with Cul-3GR18) Cul-3mds1 spermatid (with Cul-3mds1) Cul-3gft1 Cul-3gft2 Cul-3gft4 Cul-3GR18 trichogen cell | ectopic | somatic clone Cul-306430 Cul-3gft2 wing | somatic clone Cul-306430 Cul-3gft2 wing disc | somatic clone Cul-306430 Cul-3gft2 wing hair, with Scer\GAL4dpp.blk1 Cul-3Scer\UAS.cMa wing hair | somatic clone Cul-306430 Cul-3gft2 wing margin bristle, with Scer\GAL4sca-4512 Cul-3Scer\UAS.cMa wing vein | somatic clone Cul-306430 Cul-3gft2 wing vein L3 | somatic clone Cul-306430 Cul-3gft2 wing vein L4, with Scer\GAL4dpp.blk1 Cul-3Scer\UAS.cMa
Classical Alleles ( 21 )
For All Classical Alleles Show
Allele of Cul-3 Class Mutagen Stocks Known lesion Cul-3gft2 loss of function allele ethyl methanesulfonate 3 Yes Cul-306430 loss of function allele P-element activity 2 Yes Cul-3EY11031 P-element activity 2 -- Cul-3e02121 piggyBac transposase 1 -- Cul-3GR18 gamma ray 1 Yes Cul-3mds1 ethyl methanesulfonate 1 Yes Cul-3mds3 1 Yes Cul-3NP0099 1 -- Cul-3d577 PM hybrid dysgenesis 0 -- Cul-3gft1 ethyl methanesulfonate 0 -- Cul-3gft3 ethyl methanesulfonate 0 -- Cul-3gft4 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes Cul-3gft5 ethyl methanesulfonate 0 -- Cul-3gft6 ethyl methanesulfonate 0 Yes Cul-3gft7 ethyl methanesulfonate 0 -- Cul-3gft8 ethyl methanesulfonate 0 Yes Cul-3IK1 P-element activity 0 -- Cul-3mds2 0 Yes Cul-3mds4 0 Yes Cul-3mds5 0 Yes Cul-3unspecified 0 --
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Cul-3 Class Mutagen Stocks Known lesion Cul-3GD10381 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cul-3GD7408 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cul-3HM05109 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cul-3Scer\UAS.cMa in vitro construct - regulatory fusion 1 Yes Cul-3ΔC.Scer\UAS in vitro construct - regulatory fusion 1 Yes Cul-3K717R.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion 0 Yes Cul-3KK105734 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Cul-3KR.Scer\UAS.T:Zzzz\FLAG in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes Cul-3Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Cul-3Soma in vitro construct - regulatory fusion 0 Yes Cul-3Testis in vitro construct - regulatory fusion 0 Yes Cul-3WT.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(2L)64j (Ashburner, 1982, Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)A47 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A63 (Ashburner et al., 1982, Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A72 (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner et al., 1990, Fossett et al., 1994) Df(2L)A178 (Ashburner et al., 1982, Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A215 (Fossett et al., 1994) Df(2L)A217 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A220 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A245 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A260 (Ashburner et al., 1982) Df(2L)A264 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A266 (Ashburner et al., 1982, Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A267 (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner et al., 1990, Fossett et al., 1994) Df(2L)A379 (Ashburner et al., 1982, Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A400 (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) Df(2L)A445 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)Adh-nBR100 (Fossett et al., 1994) Df(2L)Adh-nBR105 (Fossett et al., 1994) Df(2L)Adh-nBR106 (Fossett et al., 1994) Df(2L)Adh-nBR107 (Fossett et al., 1994) Df(2L)Adh-nBR117 (Fossett et al., 1994) Df(2L)Adh-nBR119 (Fossett et al., 1994) Df(2L)Adh-nBR121 (Fossett et al., 1994) Df(2L)Adh-nBR122 (Fossett et al., 1994) Df(2L)Adh-nBR127 (Fossett et al., 1994) Df(2L)Adh-nBR128 (Fossett et al., 1994) Df(2L)Adh-nBR131 (Fossett et al., 1994) Df(2L)ARR1 (Ashburner, 1982, Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)b80e3 (Ashburner et al., 1982) Df(2L)b81a1 (Ashburner et al., 1982) Df(2L)b84a3 (Furukawa et al., 1992) Df(2L)b84a7 (Furukawa et al., 1992) Df(2L)b84h50 (Furukawa et al., 1992) Df(2L)b87e25 (Zhu et al., 2005) Df(2L)el28 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)fn2 (Ashburner et al., 1982, Ashburner et al., 1982) Df(2L)fn3 (Ashburner et al., 1982, Ashburner, 1982) Df(2L)fn5 (Ashburner et al., 1982, Furukawa et al., 1992) Df(2L)fn7 (Ashburner et al., 1982, Ashburner, 1982) Df(2L)fn12 (Ashburner et al., 1982, Ashburner et al., 1990) Df(2L)fn15 (Ashburner et al., 1982) Df(2L)fn30 (Ashburner et al., 1982) Df(2L)fn31 (Ashburner et al., 1982, Ashburner, 1982) Df(2L)fn36 (Ashburner et al., 1982) Df(2L)H20 (Ashburner et al., 1990) Df(2L)osp144 (Ashburner et al., 1982) Df(2L)PA4 (Ashburner et al., 1983) Df(2L)r10 (Ashburner et al., 1990, Zhu et al., 2005) Df(2L)RA5 (Ashburner et al., 1990) Df(2L)rd9 (Fergestad et al., 2010, Ashburner et al., 1990, Furukawa et al., 1992, Roote, 1996.8, Zhu et al., 2005) Df(2L)RM5 (Ashburner et al., 1990) Df(2L)RN2 (Ashburner et al., 1990) Df(2L)Scorv4 (Ashburner et al., 1983, Ashburner et al., 1990) Df(2L)Scorv10 (Ashburner et al., 1983) Df(2L)Scorv14 (Ashburner et al., 1983) Df(2L)Scorv16 (Ashburner et al., 1983, Ashburner et al., 1990) Df(2L)Scorv18 (Ashburner et al., 1983, Ashburner et al., 1990) Df(2L)Scorv19 (Ashburner et al., 1983, Ashburner et al., 1990) Df(2L)Scorv25 (Ashburner et al., 1983, Ashburner et al., 1990) Df(2L)TE35BC-1 (Ashburner, 1982) Df(2L)TE35BC-7 (Furukawa et al., 1992) Df(2L)TE35BC-28 (Furukawa et al., 1992) Df(2L)TE35D-1 (Ashburner et al., 1990) Df(2L)TE35D-2 (Ashburner et al., 1990, Furukawa et al., 1992, Roote, 1996.8) Df(2L)TE35D-3 (Ashburner et al., 1990) Df(2L)TE35D-13 (Ashburner et al., 1990, Furukawa et al., 1992, Roote, 1996.8) Df(2L)TE35D-14 (Ashburner et al., 1990) Df(2L)TE35D-19 (Ashburner et al., 1990) Df(2L)TE35D-21 (Ashburner et al., 1990, Roote, 1996.8) Df(2L)TE35D-22 (Cai et al., 2001, Ashburner et al., 1990) Df(2L)W (Ashburner et al., 1982) In(2L)C158LScorv11R (Ashburner et al., 1983) In(2L)C158LScorv17R (Ashburner et al., 1983) In(2LR)Scorv1-DV7 (Ashburner et al., 1990) T(2;3)Scorv7 (Ashburner et al., 1983, Ashburner and Harrington, 1984, Ashburner et al., 1990) T(Y;2)A80DR15P (Ashburner et al., 1982) Ts(2Lt;3Lt)TE35BC-3+Ts(2Rt;3Rt)Scorv13 (Ashburner et al., 1983) Ts(Y;2Lt)el4+Ts(YSt;2Rt)R15 (Ashburner et al., 1982)
Disrupted in	Df(2L)A48 (Spradling et al., 1999, Cai et al., 2001, Ashburner et al., 1982, Ashburner et al., 1982, BDGP Project Members, 1994-1999, Fossett et al., 1994) Df(2L)A215 (Ashburner et al., 1982) Df(2L)A246 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A263 (Ashburner et al., 1982, Furukawa et al., 1992) Df(2L)A337 (Fossett et al., 1994) Df(2L)A376 (Ashburner et al., 1982, Ashburner, 1982, Fossett et al., 1994) Df(2L)A377 (Ashburner et al., 1982, Ashburner, 1982) Df(2L)Adh-nBR101 (Fossett et al., 1994) Df(2L)Adh-nBR102 (Fossett et al., 1994) Df(2L)Adh-nBR103 (Fossett et al., 1994) Df(2L)Adh-nBR118 (Fossett et al., 1994) Df(2L)Adh-nBR120 (Fossett et al., 1994) Df(2L)Adh-nBR125 (Fossett et al., 1994) Df(2L)Adh-nBR126 (Fossett et al., 1994) Df(2L)Adh-nBR129 (Fossett et al., 1994, Roote, 1996.1.11, Roote et al., 1996, Roote, 1996.8) Df(2L)Adh-nBR130 (Fossett et al., 1994) Df(2L)b80e4 (Ashburner et al., 1990) Df(2L)do1 (Ashburner, 1982) Df(2L)ED3 (Ryder and Roote, 2004.11.10) Df(2L)ED1050 (Roote, 2004.6.24) Df(2L)el20 (Ashburner et al., 1990) Df(2L)el80f1 (Ashburner et al., 1983) Df(2L)Exel7062 (Ryder, 2004.4.26) Df(2L)Exel8034 (Ryder, 2004.4.26) Df(2L)fn1 (Ashburner et al., 1982, Ashburner et al., 1982) Df(2L)fn26 (Ashburner et al., 1982, Ashburner et al., 1990) Df(2L)fn27 (Ashburner et al., 1982, Ashburner et al., 1982, Ashburner, 1982, Ashburner et al., 1990) Df(2L)k07245-mr32 (Roote, 2000.6.13) Df(2L)n78l3 (Ashburner et al., 1982, Ashburner et al., 1990) Df(2L)noc11 (Ashburner et al., 1990, Roote, 1996.1.11, Roote, 1996.8) Df(2L)osp29 (Cai et al., 2001, Ashburner et al., 1982, Furukawa et al., 1992) Df(2L)osp38 (Ashburner et al., 1990) Df(2L)PZ06430-mr1 (Roote, 2000.6.13) Df(2L)PZ06430-mr2 (Roote, 2000.6.13) Df(2L)PZ06430-mr3 (Roote, 2000.6.13) Df(2L)PZ06430-mr4 (Roote, 2000.6.13) Df(2L)PZ06430-mr5 (Roote, 2000.6.13) Df(2L)PZ06430-mr7 (Roote, 2000.6.13) Df(2L)PZ06430-mr8 (Roote, 2000.6.13) Df(2L)PZ06430-mr9 (Roote, 2000.6.13) Df(2L)PZ06430-mr10 (Roote, 2000.6.13) Df(2L)PZ06430-mr11 (Roote, 2000.6.13) Df(2L)PZ06430-mr12 (Roote, 2000.6.13) Df(2L)PZ06430-mr13 (Roote, 2000.6.13) Df(2L)PZ06430-mr14 (Roote, 2000.6.13) Df(2L)Sco-1 (Ashburner, 1982) Df(2L)Sco7 (Ashburner et al., 1983, Ashburner et al., 1990) Df(2L)Scorv23 (Ashburner, 1982, Ashburner et al., 1983) Df(2L)SS1 (Ashburner et al., 1990) Df(2L)TE35BC-3 (Cai et al., 2001, Ashburner et al., 1982, Ashburner, 1982, Ashburner et al., 1990) Df(2L)TE35BC-4 (Ashburner et al., 1982, Ashburner, 1982, Ashburner et al., 1990) Df(2L)TE35BC-24 (Zhu et al., 2005) Df(2L)TE35BC-34 (Ashburner et al., 1990) Df(2L)TE35D-4 (Ashburner et al., 1990) Df(2L)TE35D-6 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)TE35D-15 (Ashburner et al., 1990, Furukawa et al., 1992) Df(2L)TE35D-16 (Ashburner et al., 1990) Df(2L)TE35D-18 (Furukawa et al., 1992) Df(2L)TE35D-20 (Ashburner et al., 1990) Df(2L)TE35D-23 (Ashburner et al., 1990) In(2L)75c (Spradling et al., 1999, Ashburner, 1982, BDGP Project Members, 1994-1999) In(2L)C158LScorv11R (Ashburner and Harrington, 1984) In(2L)C158LScorv17R (Ashburner and Harrington, 1984) In(2L)dpps22Ldppd36R (Ashburner et al., 1990) In(2L)el18 (Ashburner et al., 1990) In(2LR)noc4LScorv9R (Cai et al., 2001, Ashburner and Harrington, 1984) In(2LR)TE35B-6LScorv9R (Ashburner and Harrington, 1984) Tp(2;2)A446 (Ashburner et al., 1982, Ashburner et al., 1982, Fossett et al., 1994) Ts(2Lt;3Lt)TE35B-4+Ts(2Rt;3Rt)Scorv13 (Ashburner and Harrington, 1984) Ts(2Lt;3Lt)TE35BC-3+Ts(2Rt;3Rt)Scorv13 (Ashburner and Harrington, 1984)
Duplicated in	Dp(2;1)Scorv23 (Ashburner, 1982, Ashburner et al., 1983, Ashburner et al., 1990) Dp(2;2)TE35B-54L (Ashburner et al., 1990) Dp(2;3)osp3 (Ashburner et al., 1990) In(2L)C163.41LC158R (Ashburner, 1982, Ashburner et al., 1990) In(2L)Scorv17LC158R (Ashburner, 1982, Ashburner et al., 1990)
Not duplicated in	Dp(2;2)Adh3 (Ashburner, 1982) Dp(2;2)GYL (Ashburner, 1982, Ashburner et al., 1990) Dp(2;2)GYS (Ashburner, 1982)
Partially disrupted in	Df(2L)le1 (Texada et al., 2008)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK105734} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Cul-306430 No insertion of mobile activating element P{EPgy2}Cul-3EY11031 NA insertion of enhancer trap binary system P{GawB}Cul-3NP0099 No miscellaneous insertions P{}Cul-3d577 NA PBac{RB}Cul-3e02121 NA
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
activation of caspase activity inferred from mutant phenotype (Arama et al., 2007) axonogenesis inferred from mutant phenotype (Zhu et al., 2005) dendrite morphogenesis inferred from mutant phenotype (Zhu et al., 2005) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Mistry et al., 2004) protein ubiquitination inferred from mutant phenotype (Arama et al., 2007) sensory organ development inferred from mutant phenotype (Mistry et al., 2004) sperm individualization inferred from mutant phenotype (Arama et al., 2007)
Cellular Component
CV term Evidence References
Cul3-RING ubiquitin ligase complex inferred from physical interaction with klhl10 (Arama et al., 2007)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
ubiquitin-protein ligase activity non-traceable author statement (Ou et al., 2003) ubiquitin protein ligase binding inferred from electronic annotation with InterPro:IPR001373, InterPro:IPR016157, InterPro:IPR016158, InterPro:IPR019559 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
compound eye morphogenesis non-traceable author statement (Ou et al., 2003) regulation of proteolysis non-traceable author statement (Ou et al., 2003) ubiquitin-dependent protein catabolic process inferred from electronic annotation with InterPro:IPR001373, InterPro:IPR016157, InterPro:IPR016158, InterPro:IPR019559 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
nuclear ubiquitin ligase complex inferred from sequence or structural similarity with EMBL:AF083216 (FlyBase, 1992-) SCF ubiquitin ligase complex non-traceable author statement (Ou et al., 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Cul-3 allele Gene References gft1 G-sα60A (Mistry et al., 2004) gft2 G-sα60A (Mistry et al., 2004) gft4 G-sα60A (Mistry et al., 2004) gft06430 G-sα60A (Mistry et al., 2004) unspecified G-sα60A (Mistry, 1997, Mistry et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60913 DroID - A comprehensive database of gene and protein interactions. • FBgn0261268 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34896
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14531 Dere\GG24216 Dgri\GH13125 Dmoj\GI22726 Dper\GL26600 Dpse\GA16511 Dsec\GM14478 Dsim\GD21965 Dvir\GJ23288 Dwil\GK18258 Dyak\GE19410
Curated drosophilid orthologs	Dpse\GA11243   Dpse\GA16511
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11526 LH19858 FBgn0236753 FBgn0220257 FBgn0210390 FBgn0193380 FBgn0169401 JGI_V11_228373 FBgn0076526 FBgn0164182 FBgn0145453 FBgn0120602 FBgn0116348 FBgn0091558 CPIJ011310 BGIBMGA013548 ACYPI008151 ACYPI001054 GB16348 AGAP012905 AGAP008105 AAEL007187 AAEL006291 PB19854 PHUM587350 GLEAN_13922
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	9935 w*; P{UAS-gft.ΔC}3 9936 w*; P{UAS-gft.FL}3 23866 Cul-3gft2 P{neoFRT}40A/CyO 28899 y1 v1; P{TRiP.HM05109}attP2 25147 Cul-3mds1 cn1 bw1/CyO 32125 Cul-3mds3 cn1 bw1/CyO 12175 cn1 P{PZ}Cul-306430/CyO; ry506 20656 y1 w67c23; P{EPgy2}Cul-3EY11031
Harvard	- PBac{RB}Cul-3e02121
Kyoto	106883 Adhn10 Cul-3gft2 cn1 vg1/CyO
VDRC	v16331 w1118; P{GD7408}v16331 v25875 w1118; P{GD10381}v25875
	More stocks available...
Genomic Clones ( 1 )
	BACR36B06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 115 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD10516
Other clones	GH13892 MIP05150
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01565 AT02280 AT03216 AT03778 AT05034 AT07783 AT08501 AT08710 AT10339 AT12396 AT13227 AT16276 AT19493 AT19866 AT20863 AT21182 AT21933 AT25411 AT28473 GH08809 GH13215 GH13234 GH27416 LD03316 LD03964 LD29530 LD34096 LD39446 RE09378 RE10178 RE10675 RE46419 RE49322 RE51222 RE53029 RE57306 RE57323 RH27195 SD08760 SD20020
Other clones	12434 40526 40590 41989 53030 54830 57016 57039 82182 83264 156856 158042 162949 167322 167424 177130 181796 AT01026 bs02e09 bs13b05 bs24c08 bs25a04 bs28c10 bs68e10 CK01.7D.G5 CK01.145A.B8 EC13601 EC35009 EK010727 EK105808 EK113342 EK119240 EK153615 EK155328 EK168063 EK170319 EK170510 EK170544 EK179763 EK200903 EK213254 EK216728 EK218961 EK267110 EK272219 EK286318 ESG014c.E21_F10 RP001001115 RP001003037 RP001003759 RP001005284 RP001005835 RP001013081 RP001013089 RP001019588 RP001021763 RP001022187 RP001022726 RP001022839 RP001022911 RP001032872 RP001037181 RP001042146 RP001043790 RP001045568 RP001046179 RP001046359 RP001050116 RP001052587 RP001052808 RP001053710 RP001055067
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261268 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34896 318967
Antibody Information
Other Information
Discoverer
Etymology
	'guftagu' means 'private conversation' in Urdu, reflecting the experimental eavesdropping (by identification of suppressive interactions) on the interaction between gft and G-sα60A. (Mistry, 1997) Gene is named "medusa" (in Greek mythology, Medusa represents both life and death) based on the mutant phenotype that affects caspase activation. (Arama et al., 2007) The gene is named "guftagu", an Urdu word that means "private conversation", because it appears that the gene product has a "private conversation" with the activated G-sα60A signalling pathway in order to modify the phenotype caused by expression of constitutively activated G-sα60A protein. (Mistry et al., 2004)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Cul-3 cul-3 (FlyBase, 2009-)
Source for database merge of	Source for merge of: gft CG31829 (FlyBase Genome Annotators, 2009)
Additional comments	Annotations CG11861 and CG31829 merged as CG42616 in release 5.20 of the genome annotation. (FlyBase Genome Annotators, 2009) Renamed to be consistent with FlyBase nomenclature rules. (FlyBase, 2009-)
Other Comments
	Normal gft function requires an intact Nedd8 conjugation site. (Zhu et al., 2005) gft plays an essential role in both axonal arborization and proper elaboration of dendrites and may require neddylation for its proper function. (Zhu et al., 2005) gft mutant axons often stall around branching points, and it is possible that mutant neurites become truncated because of defects in arborization rather than extension. (Zhu et al., 2005) Perturbation of gft function has pleiotropic effects during development, including defects in external sensory organ development, defects patterning in the wing and notum and defects in cell growth and survival. (Mistry et al., 2004) New annotation (CG31829) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) 1 additional allele is discussed but is not named. (Ashburner et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078849 NM_165110 NM_165111 NM_165112 RefSeq (Proteins) • NP_523573 NP_723907 NP_723908 NP_723909 DDBJ / EMBL / GenBank • AA202900 AA391453 AA816692 AA949500 AA979495 AAF44933 AAF53450 AAF53451 AAN10895 AAN10896 AAX33554 AE003412 AI516836 AI944606 AI945458 AI946599 AW942138 BE662122 BE662150 BT021406 CL868471 CZ479946 G00415 L80117 Entrez Gene - A searchable database of RefSeq genes. • 34896 UniProtKB/TrEMBL • Q8IP45 Q9V475
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Cullin, N-terminal (IPR001373) Winged helix-turn-helix transcription repressor DNA-binding (IPR011991) Cullin, conserved site (IPR016157) Cullin homology (IPR016158) Cullin repeat-like-containing domain (IPR016159) Cullin protein, neddylation domain (IPR019559)
Linkouts
	BioGRID - A database of protein and genetic interactions • 60913 DroID - A comprehensive database of gene and protein interactions. • FBgn0261268 DRSC - Results from RNAi screens. • FBgn0261268 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0261268 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261268 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 216192 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34896 318967 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34896 modMine - Data generated by the modENCODE project. • FBgn0261268 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV11526 LH19858 FBgn0236753 FBgn0220257 FBgn0210390 FBgn0193380 FBgn0169401 JGI_V11_228373 FBgn0076526 FBgn0164182 FBgn0145453 FBgn0120602 FBgn0116348 FBgn0091558 CPIJ011310 BGIBMGA013548 ACYPI008151 ACYPI001054 GB16348 AGAP012905 AGAP008105 AAEL007187 AAEL006291 PB19854 PHUM587350 GLEAN_13922
Synonyms & Secondary IDs ( 30 )
Reported As
Symbol Synonym	BG:DS07851.2 (Ashburner et al., 1999.10.12, Ashburner et al., 1999) br34 (Furukawa et al., 1992, Ashburner and Harrington, 1984) CG11861 (Donaldson et al., 2004, Haerty et al., 2007, Ida et al., 2009, Perkins et al., 2009, Keleman et al., 2009.8.5) CG31829 (Arama et al., 2007, Fujiyama-Nakamura et al., 2009) CG42616 (FlyBase Genome Annotators, 2009) cul3 (Arama et al., 2007, Arama et al., 2007, Djagaeva and Doronkin, 2009, Kaplan et al., 2010, Ayyub, 2011) CUL3 (Higa et al., 2003) Cul3 (Ou et al., 2003, Ou et al., 2002, Jiang, 2002, Ou et al., 2006, Chien-Hsiang et al., 2007, Arama et al., 2007, Zhu et al., 2005, Wu et al., 2005, Wang and Price, 2008, Zhang et al., 2006, Reynolds et al., 2008, Ou et al., 2007, Chan et al., 2008, Chan et al., 2008, Du et al., 2011, Heriche et al., 2003, Rencus-Lazar et al., 2008, Kim et al., 2010) Cul-3 (Mistry et al., 2003, Hudson and Cooley, 2010, Wasbrough et al., 2010, Wasbrough et al., 2010) cul-3   CUL-3 (Kaplan et al., 2010) dCul3 (Bocca et al., 2001, Fujiyama-Nakamura et al., 2009) dCul-3 (Mistry and Skeath, 2001) DMcul-3 (Filippov et al., 2000) gft (Skeath, 2007.3.20, Moorman et al., 2006, Ayyub et al., 2005, Zhu et al., 2005, Roote, 2008.8.22, Font-Burgada et al., 2008, Ida et al., 2009, Pasyukova et al., 2004, Heriche et al., 2003, Fergestad et al., 2010, Foley et al., 2010, Beltran et al., 2007, Mandal et al., 2010) gft/dCul-3 (Mistry et al., 2004) guftagu (Heriche et al., 2003) l35Cd (Fossett et al., 1994) l(2)35Cd (Ashburner et al., 1999, The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Alexandrova et al., 1996, Roote et al., 1996, Spradling et al., 1995, ) l(2)06430   l(2)br34 (The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Ashburner et al., 1990, Ashburner et al., 1983, Ashburner, 1982, Ashburner et al., 1982, Ashburner et al., 1982, ) mds (Arama et al., 2007)
Name Synonym	CG31829   Cullin (Alexandre et al., 2008) Cullin3 (Lin et al., 2002, Zhu et al., 2005, Djagaeva and Doronkin, 2009, Djagaeva and Doronkin, 2009, Kim et al., 2010) Cullin-3 (Mistry et al., 2003, Arama et al., 2007, Hudson and Cooley, 2010, Wasbrough et al., 2010) cullin-3 (Arama et al., 2006, Arama et al., 2007, Kaplan et al., 2010) cullin3 (Wu et al., 2005, Ou et al., 2007, Texada et al., 2008) cullin 3   Cullin 3 (Mandal et al., 2010) guftagu (Mistry, 1997, Zhu et al., 2005, Ida et al., 2009, Fujiyama-Nakamura et al., 2009, Pasyukova et al., 2004, Texada et al., 2008) medusa (Arama et al., 2007)
Secondary FlyBase IDs
	FBgn0001980 FBgn0027882 FBgn0051829
References ( 106 )
Generate a list of	All references relating to this gene ( 106 )
List References by type	Research paper  ( 53 ) Supplementary material  ( 6 ) Review  ( 3 ) Personal communication to FlyBase  ( 19 ) Abstract  ( 13 ) FlyBase analysis  ( 5 ) Stock list  ( 1 ) Computer file  ( 4 ) Curated genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 10 )
	Ayyub, 2011, J. Genet. 90(2): 239--249 Cullin-5 and cullin-2 play a role in the development of neuromuscular junction and the female germ line of Drosophila. [FBrf0214802] Du et al., 2011, PLoS ONE 6(9): e24168 In Vivo RNAi Screen Reveals Neddylation Genes as Novel Regulators of Hedgehog Signaling. [FBrf0215574] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Fergestad et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(12): 5617--5621 A Drosophila behavioral mutant, down and out (dao), is defective in an essential regulator of Erg potassium channels. [FBrf0210402] Foley et al., 2010, Biol. Letts 6(1): 102--105 Does segregating variation in sexual or microhabitat preferences lead to non-random mating within a population of Drosophila melanogaster? [FBrf0209672] Hudson and Cooley, 2010, J. Cell Biol. 188(1): 29--37 Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeleton. [FBrf0209686] Kaplan et al., 2010, Dev. Cell 19(1): 160--173 Gradients of a Ubiquitin E3 Ligase Inhibitor and a Caspase Inhibitor Determine Differentiation or Death in Spermatids. [FBrf0211287] Kim et al., 2010, Dev. Biol. 346(2): 247--257 Drosophila Cand1 regulates Cullin3-dependent E3 ligases by affecting the neddylation of Cullin3 and by controlling the stability of Cullin3 and adaptor protein. [FBrf0211875] Mandal et al., 2010, J. Cell Biol. 188(4): 473--479 Metabolic control of G1-S transition: cyclin E degradation by p53-induced activation of the ubiquitin-proteasome system. [FBrf0210063] Wasbrough et al., 2010, J. Proteomics 73(11): 2171--2185 The Drosophila melanogaster sperm proteome-II (DmSP-II). [FBrf0211978] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...