FlyBase Gene Report: Dmel\Ero1L

General Information

Symbol

Dmel\Ero1L

Species

D. melanogaster

Name

Endoplasmic reticulum oxidoreductin-1-like

Annotation Symbol

CG1333

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261274

Gene Model Status

Current

Stock Availability

10 publicly available

Enzyme Name (EC)

Thiol oxidase (1.8.3.2)

Gene Summary

Endoplasmic reticulum oxidoreductin-1-like (Ero1L) encodes a thiol oxidase that plays an important role in disulfide-bond formation. It oxidizes Pdi and mediates the oxidative protein folding in the endoplasmic reticulum. [Date last reviewed: 2021-01-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

kiga

Key Links

Genomic Location

Cytogenetic map

64A9-64A9

Sequence location

3L:4,170,404..4,173,789 [+]

Recombination map

3-10

RefSeq locus

NT_037436 REGION:4170404..4173789

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables thiol oxidase activity

inferred from mutant phenotype

(Tien et al., 2008)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables FAD binding

inferred from electronic annotation with InterPro:IPR007266

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables oxidoreductase activity

inferred from sequence or structural similarity with UniProtKB:Q96HE7

(assigned by UniProt )

(UniProt Curators, 2002-)

enables protein-disulfide reductase activity

inferred from biological aspect of ancestor with PANTHER:PTN000289179

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in protein folding in endoplasmic reticulum

inferred from mutant phenotype

(Tien et al., 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in chaperone cofactor-dependent protein refolding

inferred from sequence or structural similarity with UniProtKB:Q96HE7

(assigned by UniProt )

(UniProt Curators, 2002-)

involved_in protein folding in endoplasmic reticulum

inferred from biological aspect of ancestor with PANTHER:PTN000289179

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in endoplasmic reticulum membrane

inferred from biological aspect of ancestor with PANTHER:PTN000289179

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in endoplasmic reticulum

inferred from sequence or structural similarity with UniProtKB:Q96HE7

(assigned by UniProt )

(UniProt Curators, 2002-)

Gene Group (FlyBase)

OTHER SULFUR OXIDOREDUCTASES, DISULFIDE AS ACCEPTOR

Protein Family (UniProt)

Belongs to the EROs family. (Q9V3A6)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2) (1.8.3.2)

Predictions / Assertions

Summaries

Gene Snapshot

Gene Group (FlyBase)

OTHER SULFUR OXIDOREDUCTASES, DISULFIDE AS ACCEPTOR -: The other sulfur oxidoreductases, disulfide as acceptor group include enzymes that oxidize a sulfur-containing group and reduce disulfide but which do not fit into any of the other major groups.

Protein Function (UniProtKB)

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes pdi-1, the enzyme catalyzing protein disulfide formation, in order to allow pdi-1 to sustain additional rounds of disulfide formation. Following pdi reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell (By similarity).

(UniProt, Q9V3A6)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ero1L for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V3A6)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ero1L-RB

FBtr0073217

NM_139608

2100

483

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ero1L-PB

FBpp0073073

55.7

483

5.20

NP_647865

AAN11590

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

416 (aa)

(Cabibbo et al., 2000)

Comments

External Data

Subunit Structure (UniProtKB)

May function both as a monomer and a homodimer.

(UniProt, Q9V3A6)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V3A6

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ero1L using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ero1L_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10 -- 11

embryonic/larval tracheal section

(Chung et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ero1L in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ero1L

View exonic expression by tissue for Dmel\Ero1L

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38500/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261274

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028736

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261274

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1333

Flygut - An atlas of the Drosophila adult midgut

FBgn0261274

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ero1L

Transgenic constructs containing regulatory region of Ero1L

Aberrations (Deficiencies and Duplications) ( 12 )

Inferred from experimentation ( 12 )

Gene disrupted in

Df(3L)9-7K

(Kulkarni et al., 1994)

Df(3L)A466

(Kulkarni et al., 1994)

Df(3L)E14

(Kulkarni et al., 1994)

Df(3L)Exel6100

(Ryder, 2004.4.26)

Df(3L)GN19

(Harrison et al., 1995)

Df(3L)GN34

(Harrison et al., 1995)

Df(3L)TE1

(Harrison et al., 1995, Kulkarni et al., 1994)

Df(3L)TE3

(Kulkarni et al., 1994)

Gene not disrupted in

Df(3L)C175

(Harrison et al., 1995)

Df(3L)D4

(Kulkarni et al., 1994)

Df(3L)F2

(Kulkarni et al., 1994)

Df(3L)HR298

(Harrison et al., 1995)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

viable, with Scer\GAL4^Mef2.PR

Ero1L^GD3577

viable, with Scer\GAL4^tin.CΔ4

Ero1L^GD3577

Other Phenotypes

Allele

increased cell number | adult stage | somatic clone

Ero1L^335QRS

increased cell number | somatic clone, with Scer\GAL4^unspecified

Ero1L^GD3577

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

Ero1L^GD3577

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130, Scer\GAL80^Su(H).GBE

Ero1L^GD3577

Phenotype manifest in

Allele

adult gut, with Scer\GAL4^NP5130

Ero1L^GD3577

adult gut, with Scer\GAL4^NP5130, Scer\GAL80^Su(H).GBE

Ero1L^GD3577

adult gut | somatic clone, with Scer\GAL4^unspecified

Ero1L^GD3577

dorsal head epidermis

egg chamber

Ero1L^23T

follicle cell

Ero1L^23T

head bristle

macrochaeta & thorax

wing margin

Ero1L^335QRS

wing vein

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ERO1B

13 of 15

Yes

Hsap\ERO1A

12 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ero1lb

12 of 15

Yes

Mmus\Ero1l

12 of 15

Yes

Mmus\Cox5a

1 of 15

Rattus norvegicus (Norway rat) (2)

Rnor\Ero1b

12 of 13

Yes

Rnor\Ero1a

10 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\ero1a

12 of 12

Yes

Xtro\ero1b

9 of 12

Yes

Danio rerio (Zebrafish) (2)

Drer\ero1a

11 of 15

Yes

Drer\ero1b

11 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\ero-1

14 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\ERO1

7 of 9

Yes

Atha\ERO2

7 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ERO1

12 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\ero12

9 of 12

Yes

Spom\ero11

7 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ERO1B; endoplasmic reticulum oxidoreductase 1 beta

13 of 15

615437

ERO1A; endoplasmic reticulum oxidoreductase 1 alpha

12 of 15

615435

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Ero1L

suppressible

cnc

(Wang et al., 2015)

Ero1L

suppressible

PEK

(Wang et al., 2015)

Ero1L

suppressible

Xbp1

(Wang et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

Ero1L

(Tien et al., 2008)

External Data

Subunit Structure (UniProtKB)

May function both as a monomer and a homodimer.

(UniProt, Q9V3A6 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261274

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Protein processing in endoplasmic reticulum

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-10

Cytogenetic map

64A9-64A9

Sequence location

3L:4,170,404..4,173,789 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

64A9-64A9

Limits computationally determined from genome sequence between P{PZ}l(3)rG166^rG166 and P{PZ}sinu⁰⁶⁵²⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-10

(FlyBase, 2016)

3-8.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

42698

y¹ w^*; P{UAS-Ero1L.T}6B

27832

w¹¹¹⁸; Mi{ET1}Ero1L^MB10117

5180

bw¹; Ero1L^B3 st¹/TM3, Sb¹

5181

bw¹; Ero1L^C8 st¹/TM3, Sb¹

77677

w¹¹¹⁸; PBac{IT.GAL4}Ero1L^4093-G4/TM6B, Tb¹

64674

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05709}attP40

29300

y¹ w^*; P{EP}Ero1L^G18511/TM3, Sb¹ Ser¹

Kyoto

108087

bw¹; Ero1L^B3 st¹/TM3, Sb¹

VDRC

v110454

P{KK101914}VIE-260B

v51169

w¹¹¹⁸; P{GD3577}v51169

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (93)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD02945

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261274

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261274

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38500

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ero1L CG1333

(Gabrielian, 1999.11)

Source for identity of: Ero1L kiga

(Tien, 2006.2.28)

Source for database merge of

Source for merge of: Ero1L l(3)64Ag

(Tien et al., 2008)

Additional comments

Other Comments

Ero1L is specifically required for lateral inhibition but not for cell fate decisions and differentiation.

(Tien et al., 2008)

Discussed during genetic analysis of the Ras64B-Rop region.

(Harrison et al., 1995)

Origin and Etymology

Discoverer

Etymology

Named 'kiga', which is phonetic for 'notched' in Taiwanese.

(Tien et al., 2008)

Identification

External Crossreferences and Linkouts ( 34 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38500

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V3A6

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V3A6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V3A6

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261274

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38500/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261274

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ero1L_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028736

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261274

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1333

Flygut - An atlas of the Drosophila adult midgut

FBgn0261274

FlyMine - An integrated database for Drosophila genomics

FBgn0261274

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38500

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1333

MARRVEL_MODEL - MARRVEL (model organism gene)

38500

modMine - A data warehouse for the modENCODE project

FBgn0261274

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261274

DRSC - Results frm RNAi screens

FBgn0261274

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Protein processing in endoplasmic reticulum

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

CG1333

(Kumar et al., 2016, Park et al., 2014, Cammarato et al., 2011, Keleman et al., 2009.8.5, Perkins et al., 2009)

ERO1-L

(Cabibbo et al., 2000)

Ero1

(Sepúlveda-Salinas and Ramos-Castañeda, 2017)

Ero1L

(Sekiya et al., 2017, Clandinin and Owens, 2016-, Wang et al., 2015, Park et al., 2014, Kwon et al., 2013, Hazelett et al., 2009, Tien et al., 2008)

Ero1l

(Malzer et al., 2013)

ero1L

(Ryoo, 2015, Wang et al., 2015)

kiga

(Tien et al., 2008, Tien, 2006.2.28, Tien et al., 2006)

l(3)64Ag

l(3)SH22

(Harrison et al., 1995)

Name Synonyms

Endoplasmic reticulum oxidoreductin-1-like

(Tien et al., 2008)