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Gene Dmel\proPO-A1

General Information
Symbol	Dmel\proPO-A1	Species	D. melanogaster
Name	prophenol oxidase A1	Annotation symbol	CG42639
Feature type	protein_coding_gene	FlyBase ID	FBgn0261362
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	CG5779, Mox, Dox-A1, PPO1, Propo, A1, pro-PO, DoxA1, proPO54, PPO
Genomic Location
Chromosome (arm)	2R	Recombination map	2-79.6
Cytogenetic map	54F6-54F6	Sequence location	2R:13,774,725..13,777,478 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene prophenol oxidase A1 is referred to in FlyBase by the symbol Dmel\proPO-A1 (CG42639, FBgn0261362). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: L-DOPA monooxygenase activity; catechol oxidase activity; dopamine monooxygenase activity; monophenol monooxygenase activity. It is reported to be involved in the biological process: dopamine metabolic process. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Hemocyanin, C-terminal; Hemocyanin, N-terminal; Hemocyanin/hexamerin; Hemocyanin/hexamerin middle domain; Immunoglobulin E-set; Tyrosinase; Uncharacterised domain, di-copper centre. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages, during late larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult heart, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult brain, larval trachea. Comments on Affy2 ProbeSet: ProbeSet 1635494_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of proPO-A1. Gene sequence location is 2R:13774725..13777478.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Dox-A1: Diphenol oxidase A1 subunit Enzyme detectable in embryos at ten hours and remains throughout development and adult life. A1 band more intense than those of subunits A2 and A3.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 54F6-54F6
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	2-79.6 (Asada et al., 1993)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\proPO-A1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) proPO-A1-RA FBtr0302291  NM_057464   2338   690
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein proPO-A1-PA   FBpp0291497   79.1   690   6.57     NP_476812   AAF57775
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Immunoglobulin E-set (IPR014756) Hemocyanin/hexamerin (IPR013788) Hemocyanin, N-terminal (IPR005204) Hemocyanin/hexamerin middle domain (IPR000896) Uncharacterised domain, di-copper centre (IPR008922) Tyrosinase (IPR002227) Hemocyanin, C-terminal (IPR005203)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA816984   AE013599 AAF57775   AC004640   AI107318   AI107728   AI947054   AY060617   BI214919   BI216824   BI241542   BI243715   BI361653   BI369509   BI482709   BI484732   BI566636   BI573407   BI573408   BI573409   BI573410   BI576652   BI579706   BI582314   BI582315   BI583721   BI584787   BI584960   BI587863   BI606027   BI607274   BI607853   BI608188   BI608657   BI610063   BI611191   BI611483   BI611613   BI611642   BI613003   BI614166   BI614485   BI614795   BI616744   BI619301   BI620556   BI623727   BI626040   BI628751   BI629114   BI631586   BI634998   CO289996   CO315875   EC070306   EC079715   EC200769   EC233060   EC244956   EC249767   GH749482   GH753329   GH754580   GH755239   GH758897   GH759118   GH770630   GH772415   GH772666   GH776791   GH781158   GH783432   GH784491   GH785680   GH790327   GH792137   GH792693   GH794147   GH794951   GH797522   GH798120   GH803196   GH804038   GH805697   GH807531   GH808324   GH812878   GH813272   GH815926   GH818156   GH819648   GH821167   GH822483   GH823324   GH828569   GH828967   GH832520   GH833030   GH835287   GH836960   GH837059   GH847043   GH848200   GH849421   GH853985   GH854171   GH854311   GH855293   GH856005   GH857700   GH857840   GH860079   GH861815   GH862764   GH865793   GH869598   GH870617   GH872962   GH874911   GH875526   GH875882   GH878203   GH879690   GH883725   GH886016   GH886907   GH887325   GH888589   GH889989   GH891203   GH891351   GH891514   GH891819   GH893133   GH893469   GH897192   GH901090   GH902360   GH904872   GH908058   GH909350   GH912246   GH916779   GH916837   GH917962   GH919464   GH919756   GH920171   GH920559   GH921254   GH923521   GH924582   GH931079   GH931185   GH932011   GH932306   GH932467   GH935706   GH940667
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K2W6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000435805 2R:13,774,335..13,774,665 evidence=Reporter construct (in vivo) (Gajewski et al., 2007, Halfon, 2012.6.19) regulatory region FBsf0000436230 2R:13,777,496..13,777,761 evidence=Reporter construct (in vivo) (Ferjoux et al., 2007, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 12   procrystal cell   (Fisher et al., 2012) embryonic stage 13 -- 16   crystal cell   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
extracellular region inferred from direct assay (Karlsson et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult heart, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult brain, larval trachea. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    13.3 Larval Hindgut    75.8 Larval Malpighian Tubules    25.1 Larval Fat Body    96.4 Larval Salivary Gland    15.3 Larval Trachea    321.05 Larval Carcass    646.625 Adult Head    438.5 Adult Eye    57.3 Adult Brain    166.1 Adult Thoracic-Abdominal Ganglion    89.8 Adult Crop    29.8 Adult Midgut    2.4 Adult Hindgut    6.9 Adult Malpighian Tubules    12.5 Adult Fat Body    69.6 Adult Salivary Gland    13.9 Adult Heart    1904.875 Adult VirginFemale Spermatheca    39.5 Adult InseminatedFemale Spermatheca    17.6 Adult Ovary    2.9 Adult Testis    78.1 Adult Male Accessory Gland    11.6 Adult Carcass    545.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    13.3 Larval Hindgut    75.8 Larval Malpighian Tubules    25.1 Larval Fat Body    96.4 Larval Salivary Gland    15.3 Larval Trachea    321.05 Larval Carcass  (646.625) Adult Head    438.5 Adult Eye    57.3 Adult Brain    166.1 Adult Thoracic-Abdominal Ganglion    89.8 Adult Crop    29.8 Adult Midgut    2.4 Adult Hindgut    6.9 Adult Malpighian Tubules    12.5 Adult Fat Body    69.6 Adult Salivary Gland    13.9 Adult Heart  (1904.875) Adult VirginFemale Spermatheca    39.5 Adult InseminatedFemale Spermatheca    17.6 Adult Ovary    2.9 Adult Testis    78.1 Adult Male Accessory Gland    11.6 Adult Carcass  (545.6) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    13.3 Larval Hindgut    75.8 Larval Malpighian Tubules    25.1 Larval Fat Body    96.4 Larval Salivary Gland    15.3 Larval Trachea    321.05 Larval Carcass    646.625 Adult Head    438.5 Adult Eye    57.3 Adult Brain    166.1 Adult Thoracic-Abdominal Ganglion    89.8 Adult Crop    29.8 Adult Midgut    2.4 Adult Hindgut    6.9 Adult Malpighian Tubules    12.5 Adult Fat Body    69.6 Adult Salivary Gland    13.9 Adult Heart  (1904.875) Adult VirginFemale Spermatheca    39.5 Adult InseminatedFemale Spermatheca    17.6 Adult Ovary    2.9 Adult Testis    78.1 Adult Male Accessory Gland    11.6 Adult Carcass    545.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    13.3 Larval Hindgut    75.8 Larval Malpighian Tubules    25.1 Larval Fat Body    96.4 Larval Salivary Gland    15.3 Larval Trachea    321.05 Larval Carcass    646.625 Adult Head    438.5 Adult Eye    57.3 Adult Brain    166.1 Adult Thoracic-Abdominal Ganglion    89.8 Adult Crop    29.8 Adult Midgut    2.4 Adult Hindgut    6.9 Adult Malpighian Tubules    12.5 Adult Fat Body    69.6 Adult Salivary Gland    13.9 Adult Heart    1904.875 Adult VirginFemale Spermatheca    39.5 Adult InseminatedFemale Spermatheca    17.6 Adult Ovary    2.9 Adult Testis    78.1 Adult Male Accessory Gland    11.6 Adult Carcass    545.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    13.3 Larval Hindgut    75.8 Larval Malpighian Tubules    25.1 Larval Fat Body    96.4 Larval Salivary Gland    15.3 Larval Trachea    321.05 Larval Carcass    646.625 Adult Head    438.5 Adult Eye    57.3 Adult Brain    166.1 Adult Thoracic-Abdominal Ganglion    89.8 Adult Crop    29.8 Adult Midgut    2.4 Adult Hindgut    6.9 Adult Malpighian Tubules    12.5 Adult Fat Body    69.6 Adult Salivary Gland    13.9 Adult Heart    1904.875 Adult VirginFemale Spermatheca    39.5 Adult InseminatedFemale Spermatheca    17.6 Adult Ovary    2.9 Adult Testis    78.1 Adult Male Accessory Gland    11.6 Adult Carcass    545.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    13.3 Larval Hindgut    75.8 Larval Malpighian Tubules    25.1 Larval Fat Body    96.4 Larval Salivary Gland    15.3 Larval Trachea    321.05 Larval Carcass  646.625 Adult Head    438.5 Adult Eye    57.3 Adult Brain    166.1 Adult Thoracic-Abdominal Ganglion    89.8 Adult Crop    29.8 Adult Midgut    2.4 Adult Hindgut    6.9 Adult Malpighian Tubules    12.5 Adult Fat Body    69.6 Adult Salivary Gland    13.9 Adult Heart  (1904.875) Adult VirginFemale Spermatheca    39.5 Adult InseminatedFemale Spermatheca    17.6 Adult Ovary    2.9 Adult Testis    78.1 Adult Male Accessory Gland    11.6 Adult Carcass  545.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    13.3 Larval Hindgut    75.8 Larval Malpighian Tubules    25.1 Larval Fat Body    96.4 Larval Salivary Gland    15.3 Larval Trachea    321.05 Larval Carcass    646.625 Adult Head    438.5 Adult Eye    57.3 Adult Brain    166.1 Adult Thoracic-Abdominal Ganglion    89.8 Adult Crop    29.8 Adult Midgut    2.4 Adult Hindgut    6.9 Adult Malpighian Tubules    12.5 Adult Fat Body    69.6 Adult Salivary Gland    13.9 Adult Heart  (1904.875) Adult VirginFemale Spermatheca    39.5 Adult InseminatedFemale Spermatheca    17.6 Adult Ovary    2.9 Adult Testis    78.1 Adult Male Accessory Gland    11.6 Adult Carcass    545.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    16.975 Larval Midgut    13.3 Larval Hindgut    75.8 Larval Malpighian Tubules    25.1 Larval Fat Body    96.4 Larval Salivary Gland    15.3 Larval Trachea    321.05 Larval Carcass    646.625 Adult Head    438.5 Adult Eye    57.3 Adult Brain    166.1 Adult Thoracic-Abdominal Ganglion    89.8 Adult Crop    29.8 Adult Midgut    2.4 Adult Hindgut    6.9 Adult Malpighian Tubules    12.5 Adult Fat Body    69.6 Adult Salivary Gland    13.9 Adult Heart    1904.875 Adult VirginFemale Spermatheca    39.5 Adult InseminatedFemale Spermatheca    17.6 Adult Ovary    2.9 Adult Testis    78.1 Adult Male Accessory Gland    11.6 Adult Carcass    545.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    438.5   NA  Eye    57.3   NA  Brain    166.1   no informative data  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    89.8   NA  Crop    no informative data   no informative data  Midgut    2.4   75.8  Hindgut    6.9   no informative data  Malpighian Tubules    no informative data   96.4  Fat Body    69.6   no informative data  Salivary Gland    no informative data   NA  Heart    1904.875   321.05  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    2.9   NA  Testis    78.1   NA  Male Accessory Gland    no informative data   646.625  Carcass    545.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    14 head, virgin 4-day female    66 head, virgin 20-day female    26 head, mated 1-day female    29 head, mated 4-day female    95 head, mated 20-day female    47 head, mated 1-day male    14 head, mated 4-day male    132 head, mated 20-day male    58 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    8 digestive system, 4-day adult    2 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    2 carcass, 1-day adult    20 carcass, 4-day adult    18 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    4 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    14 head, virgin 4-day female  (66) head, virgin 20-day female  26 head, mated 1-day female  (29) head, mated 4-day female  (95) head, mated 20-day female  (47) head, mated 1-day male    14 head, mated 4-day male  (132) head, mated 20-day male  (58) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    8 digestive system, 4-day adult    2 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    2 carcass, 1-day adult    20 carcass, 4-day adult    18 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    4 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    14 head, virgin 4-day female    66 head, virgin 20-day female    26 head, mated 1-day female    29 head, mated 4-day female    95 head, mated 20-day female    47 head, mated 1-day male    14 head, mated 4-day male  (132) head, mated 20-day male    58 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    8 digestive system, 4-day adult    2 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    2 carcass, 1-day adult    20 carcass, 4-day adult    18 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    4 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    14 head, virgin 4-day female    66 head, virgin 20-day female    26 head, mated 1-day female    29 head, mated 4-day female    95 head, mated 20-day female    47 head, mated 1-day male    14 head, mated 4-day male    132 head, mated 20-day male    58 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    8 digestive system, 4-day adult    2 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    2 carcass, 1-day adult    20 carcass, 4-day adult    18 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    4 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    14 head, virgin 4-day female    66 head, virgin 20-day female    26 head, mated 1-day female    29 head, mated 4-day female    95 head, mated 20-day female    47 head, mated 1-day male    14 head, mated 4-day male    132 head, mated 20-day male    58 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    8 digestive system, 4-day adult    2 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    2 carcass, 1-day adult    20 carcass, 4-day adult    18 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    4 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    14 head, virgin 4-day female  (66) head, virgin 20-day female  26 head, mated 1-day female  29 head, mated 4-day female  (95) head, mated 20-day female  (47) head, mated 1-day male    14 head, mated 4-day male  (132) head, mated 20-day male  (58) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    8 digestive system, 4-day adult    2 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    2 carcass, 1-day adult    20 carcass, 4-day adult    18 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    4 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    14 head, virgin 4-day female    66 head, virgin 20-day female    26 head, mated 1-day female    29 head, mated 4-day female    95 head, mated 20-day female    47 head, mated 1-day male    14 head, mated 4-day male  132 head, mated 20-day male    58 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    8 digestive system, 4-day adult    2 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    2 carcass, 1-day adult    20 carcass, 4-day adult    18 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    4 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    1 central nervous system, pupae P8    0 head, virgin 1-day female    14 head, virgin 4-day female    66 head, virgin 20-day female    26 head, mated 1-day female    29 head, mated 4-day female    95 head, mated 20-day female    47 head, mated 1-day male    14 head, mated 4-day male    132 head, mated 20-day male    58 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    8 digestive system, 4-day adult    2 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    6 carcass, larvae L3 wandering    2 carcass, 1-day adult    20 carcass, 4-day adult    18 carcass, 20-day adult    2 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    4 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages, during late larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    13 embryo 08-10hr    38 embryo 10-12hr    89 embryo 12-14hr    96 embryo 14-16hr    110 embryo 16-18hr    76 embryo 18-20hr    8 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    4 larva L2    24 larva L3 12hr old    24 larva L3 puffstage 1-2    110 larva L3 puffstage 3-6    50 larva L3 puffstage 7-9    13 white prepupae new    13 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    3 adult male 05day    17 adult male 30day    4 adult female 01day    2 adult female 05day    6 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    13 embryo 08-10hr  (38) embryo 10-12hr  (89) embryo 12-14hr  (96) embryo 14-16hr  (110) embryo 16-18hr  (76) embryo 18-20hr    8 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    4 larva L2    24 larva L3 12hr old    24 larva L3 puffstage 1-2  (110) larva L3 puffstage 3-6  (50) larva L3 puffstage 7-9    13 white prepupae new    13 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    3 adult male 05day    17 adult male 30day    4 adult female 01day    2 adult female 05day    6 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    13 embryo 08-10hr    38 embryo 10-12hr    89 embryo 12-14hr    96 embryo 14-16hr  (110) embryo 16-18hr    76 embryo 18-20hr    8 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    4 larva L2    24 larva L3 12hr old    24 larva L3 puffstage 1-2  (110) larva L3 puffstage 3-6    50 larva L3 puffstage 7-9    13 white prepupae new    13 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    3 adult male 05day    17 adult male 30day    4 adult female 01day    2 adult female 05day    6 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    13 embryo 08-10hr    38 embryo 10-12hr    89 embryo 12-14hr    96 embryo 14-16hr    110 embryo 16-18hr    76 embryo 18-20hr    8 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    4 larva L2    24 larva L3 12hr old    24 larva L3 puffstage 1-2    110 larva L3 puffstage 3-6    50 larva L3 puffstage 7-9    13 white prepupae new    13 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    3 adult male 05day    17 adult male 30day    4 adult female 01day    2 adult female 05day    6 adult female 30day    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    13 embryo 08-10hr    38 embryo 10-12hr    89 embryo 12-14hr    96 embryo 14-16hr    110 embryo 16-18hr    76 embryo 18-20hr    8 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    4 larva L2    24 larva L3 12hr old    24 larva L3 puffstage 1-2    110 larva L3 puffstage 3-6    50 larva L3 puffstage 7-9    13 white prepupae new    13 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    3 adult male 05day    17 adult male 30day    4 adult female 01day    2 adult female 05day    6 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    13 embryo 08-10hr  (38) embryo 10-12hr  (89) embryo 12-14hr  (96) embryo 14-16hr  (110) embryo 16-18hr  (76) embryo 18-20hr    8 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    4 larva L2    24 larva L3 12hr old    24 larva L3 puffstage 1-2  (110) larva L3 puffstage 3-6  (50) larva L3 puffstage 7-9    13 white prepupae new    13 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    3 adult male 05day    17 adult male 30day    4 adult female 01day    2 adult female 05day    6 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    13 embryo 08-10hr    38 embryo 10-12hr    89 embryo 12-14hr    96 embryo 14-16hr  110 embryo 16-18hr    76 embryo 18-20hr    8 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    4 larva L2    24 larva L3 12hr old    24 larva L3 puffstage 1-2  110 larva L3 puffstage 3-6    50 larva L3 puffstage 7-9    13 white prepupae new    13 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    3 adult male 05day    17 adult male 30day    4 adult female 01day    2 adult female 05day    6 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    13 embryo 08-10hr    38 embryo 10-12hr    89 embryo 12-14hr    96 embryo 14-16hr    110 embryo 16-18hr    76 embryo 18-20hr    8 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    4 larva L2    24 larva L3 12hr old    24 larva L3 puffstage 1-2    110 larva L3 puffstage 3-6    50 larva L3 puffstage 7-9    13 white prepupae new    13 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    3 adult male 05day    17 adult male 30day    4 adult female 01day    2 adult female 05day    6 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    25 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    35 wing disc CME-W2    5 wing disc ML-DmD8    0 wing disc ML-DmD9    3 wing disc ML-DmD16-c3    358 wing disc ML-DmD21    40 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    25 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1  (35) wing disc CME-W2    5 wing disc ML-DmD8    0 wing disc ML-DmD9    3 wing disc ML-DmD16-c3  (358) wing disc ML-DmD21  (40) wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    25 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    35 wing disc CME-W2    5 wing disc ML-DmD8    0 wing disc ML-DmD9    3 wing disc ML-DmD16-c3  (358) wing disc ML-DmD21    40 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    25 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    35 wing disc CME-W2    5 wing disc ML-DmD8    0 wing disc ML-DmD9    3 wing disc ML-DmD16-c3    358 wing disc ML-DmD21    40 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    25 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    35 wing disc CME-W2    5 wing disc ML-DmD8    0 wing disc ML-DmD9    3 wing disc ML-DmD16-c3    358 wing disc ML-DmD21    40 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    25 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1  (35) wing disc CME-W2    5 wing disc ML-DmD8    0 wing disc ML-DmD9    3 wing disc ML-DmD16-c3  (358) wing disc ML-DmD21  (40) wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    25 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    35 wing disc CME-W2    5 wing disc ML-DmD8    0 wing disc ML-DmD9    3 wing disc ML-DmD16-c3  (358) wing disc ML-DmD21    40 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    25 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    35 wing disc CME-W2    5 wing disc ML-DmD8    0 wing disc ML-DmD9    3 wing disc ML-DmD16-c3    358 wing disc ML-DmD21    40 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    168 Cadmium 50 mM 12 hrs, larvae L3    79 Cadmium 50 mM 48 hrs, 4-day adult    79 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    162 Ethanol 10% 3 hrs, larvae L3    70 Caffeine 1.5 mg/ml 4 hrs, larvae L3    86 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    187 Rotenone 8 μg 12 hrs, larvae L3    124 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult  (35) Cadmium 50 mM 6 hrs, larvae L3  (168) Cadmium 50 mM 12 hrs, larvae L3  (79) Cadmium 50 mM 48 hrs, 4-day adult  (79) Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3  (162) Ethanol 10% 3 hrs, larvae L3  (70) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (86) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3  (187) Rotenone 8 μg 12 hrs, larvae L3  (124) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3  (168) Cadmium 50 mM 12 hrs, larvae L3    79 Cadmium 50 mM 48 hrs, 4-day adult    79 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3  (162) Ethanol 10% 3 hrs, larvae L3    70 Caffeine 1.5 mg/ml 4 hrs, larvae L3    86 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3  (187) Rotenone 8 μg 12 hrs, larvae L3  (124) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    168 Cadmium 50 mM 12 hrs, larvae L3    79 Cadmium 50 mM 48 hrs, 4-day adult    79 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    162 Ethanol 10% 3 hrs, larvae L3    70 Caffeine 1.5 mg/ml 4 hrs, larvae L3    86 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    187 Rotenone 8 μg 12 hrs, larvae L3    124 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    168 Cadmium 50 mM 12 hrs, larvae L3    79 Cadmium 50 mM 48 hrs, 4-day adult    79 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    162 Ethanol 10% 3 hrs, larvae L3    70 Caffeine 1.5 mg/ml 4 hrs, larvae L3    86 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    187 Rotenone 8 μg 12 hrs, larvae L3    124 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult  (35) Cadmium 50 mM 6 hrs, larvae L3  (168) Cadmium 50 mM 12 hrs, larvae L3  (79) Cadmium 50 mM 48 hrs, 4-day adult  (79) Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3  (162) Ethanol 10% 3 hrs, larvae L3  (70) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (86) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3  (187) Rotenone 8 μg 12 hrs, larvae L3  (124) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3  (168) Cadmium 50 mM 12 hrs, larvae L3    79 Cadmium 50 mM 48 hrs, 4-day adult    79 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3  (162) Ethanol 10% 3 hrs, larvae L3    70 Caffeine 1.5 mg/ml 4 hrs, larvae L3    86 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3  (187) Rotenone 8 μg 12 hrs, larvae L3  124 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    35 Cadmium 50 mM 6 hrs, larvae L3    168 Cadmium 50 mM 12 hrs, larvae L3    79 Cadmium 50 mM 48 hrs, 4-day adult    79 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    162 Ethanol 10% 3 hrs, larvae L3    70 Caffeine 1.5 mg/ml 4 hrs, larvae L3    86 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    10 Rotenone 2 μg 12 hrs, larvae L3    187 Rotenone 8 μg 12 hrs, larvae L3    124 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
External Data & Images
Linkouts	BDGP expression data • GH04080 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0261362
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable proPO-A1GM95 viable, with Scer\GAL4pnr-MD237 proPO-A1GD11763 Other Phenotypes Allele electrophoretic variant proPO-A1F electrophoretic variant | slow proPO-A1S Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of proPO-A1 Class Mutagen Stocks Known lesion proPO-A1F natural population 0 -- proPO-A1GM95 natural population 0 -- proPO-A1S natural population 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of proPO-A1 Class Mutagen Stocks Known lesion proPO-A1Scer\UAS.cTa in vitro construct - regulatory fusion 6 Yes proPO-A1GD11763 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes proPO-A1KK106290 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes proPO-A1Scer\UAS.cNa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)PC4 (Asada et al., 1993) Df(2R)Pcl11B (Asada et al., 1993)
Not disrupted in	Df(2R)Pcl-W5 (Asada et al., 1993)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11763} NA P{KK106290} NA P{UAS-proPO} NA P{UAS-proPO-A1.N} NA reporter construct P{proPO-A1-lacZ.F} No
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
catechol oxidase activity inferred from direct assay (Asada and Sezaki, 1999) dopamine monooxygenase activity inferred from direct assay (Li et al., 2012) L-DOPA monooxygenase activity inferred from direct assay (Li et al., 2012) monophenol monooxygenase activity inferred from direct assay (Asada and Sezaki, 1999, Li et al., 2012)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
extracellular region inferred from direct assay (Karlsson et al., 2004)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
dopamine metabolic process inferred by curator from GO:0036264 (Li et al., 2012) metabolic process inferred from electronic annotation with InterPro:IPR002227 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
proPO-A1 - Adh experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Argk experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - CG11060 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) proPO-A1 - CG16826 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - CG31343 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - CG31445 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) proPO-A1 - CG32473 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - CG34205 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - CG3544 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) proPO-A1 - CG4390 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) proPO-A1 - CG4810 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) proPO-A1 - CG5500 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) proPO-A1 - CG9890 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) proPO-A1 - Fer1HCH experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Fer2LCH experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Iap2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) proPO-A1 - Lsp2 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Rfabg experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Scp1 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Tsf1 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Yp1 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Yp2 experimental knowledge based (Guruharsha et al., 2011) proPO-A1 - Yp3 experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions proPO-A1 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62729 DroID - A comprehensive database of gene and protein interactions. • FBgn0261362 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37044
Orthologs
OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG670V2T Dipteran inclusive ortholog search EOG6Z62WR Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search EOG6DFN3J Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG670V2T)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\proPO-A1     Drosophila simulans   Dsim\GD25430     Drosophila erecta   Dere\GG21008     Drosophila yakuba   Dyak\GE13951     Drosophila ananassae   Dana\GF13216     Drosophila pseudoobscura pseudoobscura   Dpse\GA19121     Drosophila persimilis   Dper\GL10548     Drosophila willistoni   Dwil\GK22067     Drosophila virilis   Dvir\GJ22164     Drosophila mojavensis   Dmoj\GI19287     Drosophila grimshawi   Dgri\GH22590     Drosophila grimshawi   Dgri\GH21740
Orthologs in non-Drosophila Dipterans (EOG6Z62WR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\PPO2   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011763   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011764   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014837   Aedes aegypti Yellow fever mosquito  Aaeg\PPO4   Aedes aegypti Yellow fever mosquito  Aaeg\PPO7   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL015113   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006877   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL015116   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010919   Aedes aegypti Yellow fever mosquito  Aaeg\ProPO1   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013496   Anopheles gambiae Malaria mosquito  Agam\PPO2   Anopheles gambiae Malaria mosquito  Agam\AGAP012941   Anopheles gambiae Malaria mosquito  Agam\AGAP004979   Anopheles gambiae Malaria mosquito  Agam\PPO3   Anopheles gambiae Malaria mosquito  Agam\PPO5   Anopheles gambiae Malaria mosquito  Agam\ppo8   Anopheles gambiae Malaria mosquito  Agam\PPO7   Anopheles gambiae Malaria mosquito  Agam\PPO4   Anopheles gambiae Malaria mosquito  Agam\PPO6   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005078   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005187   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ016566   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009877   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ016563   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009878   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ016565
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (EOG6DFN3J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_326755 Y
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	5867 w*; P{UAS-proPO}2 5868 w*; P{UAS-proPO}3 5866 P{UAS-proPO}X, y1 w*
Kyoto	108469 w*; P{UAS-proPO}2 108470 w*; P{UAS-proPO}3 108468 P{UAS-proPO}X, y1 w*
VDRC	v107599 P{KK106290}VIE-260B v42048 w1118; P{GD11763}v42048 v42049 w1118; P{GD11763}v42049
Genomic Clones ( 2 )
	BACR20E20 BACR22L21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 154 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH04080
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH04119 GH05556 LD21933 RE21071 RE24106 RE38637 RE41433 RE45969 RE55716 RE65437 RE67877 RE73778 RH11821 RH12368 RH13269 RH14935 RH17352 RH17758 RH17971 RH18015 RH20283 RH20284 RH22396 RH24064 RH24312 RH28756 RH33254 RH36337 RH39991 RH42053 RH43476 RH43859 RH44264 RH46807 RH50311 RH51725 RH56057 RH57285 RH57928 RH61184 RH66566 RH72106 RH74302
Other clones	16217 36596 65179 160153 bs34e07 CK01.93B.C5 CK01.116D.C4 EK072811 EK262913 RH09052 RH09053 RP003017136 RP003019268 RP003037645 RP003046686 RP003048148 RP003050074 RP003052460 RP003055348 RP003064876 RP003069928 RP003072749 RP003075180 RP003081931 RP003091333 RP003103591 RP003132140 RP003132281 RP003133138 RP003138517 RP003145763 RP003150213 RP003151297 RP003156973 RP003170563 RP003194799 RP003195036 RP003199558 RP003202263 RP003206708 RP003210740 RP003214276 RP003221159 RP003229232 RP003234851 RP003248782 RP003254651 RP003258904 RP003259175 RP003265468 RP003271558 RP003274492 RP003280262 RP003287060 RP003290832 RP003294830 RP003296251 RP003300815 RP003303540 RP003310094 RP003314382 RP003318401 RP003323970 RP003326483 RP003337670 RP003342141 RP003343921 RP003349362 RP003352230 RP003382218 RP003389541 RP003404573 RP003406947 RP003421861 RP003444383 RP003450955 RP003470032 RP003476308 RP003480102 RP003481011 RP003482696 RP003489397 RP003490638 RP003492428 RP003492850 RP003507827 RP003511019 RP003515597 RP003520763 RP003521171 RP003528020 RP003537887 RP003538920 RP003544290 RP003557150 RP003564894 RP003581429 RP003585418 RP003590171 RP003590730 RP003593648 RP003600717 RP003608972 RP003613239 RP003631741 RP003648182 RP003669971 RP003671905 RP003676093 RP003676577
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261362 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37044
Antibody Information
	polyclonal (Li et al., 2012)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: proPO-A1 CG42639 (Fujimoto et al., 1995, FlyBase, 1992-)
Source for database merge of
Additional comments	FlyBase curator comment: The Black cells (Bc) mutation had incorrectly been merged with the gene corresponding to the CG5779 annotation in FlyBase (this gene encodes the A1 component of phenol oxidase originally described in FBrf0017315) under the single gene record FBgn0000165. The confusion probably arose because phenol oxidase activity is reduced in Bc heterozygous larval cell-free extracts and undetectable in homozygous larval cell-free extracts and because the Bc mutation and the gene corresponding to the CG5779 annotation map to the same location (2-80.6). FBrf0035582 suggests that the primary defect in Bc mutants involves the loss of the physical barrier between phenol oxidase and its substrate in the crystal cells, and that the Bc[+] product is required for binding the substrate in the paracrystalline structures of the crystal cells so that it is inaccessible to the phenol oxidase enzyme (rather than Bc directly encoding a phenol oxidase). The Bc mutant locus and the gene corresponding to the CG5779 annotation have thus been split into two separate genes in FlyBase (release FB_2009_09). The gene corresponding to the CG5779 annotation has been called both "Dox-A1, Diphenol oxidase A1 subunit" and "Mox, Monophenol oxidase" in the literature. The gene product has been shown to have both monophenol oxidase and diphenol oxidase activity (FBrf0058767). To avoid confusion, the name used for this gene in FlyBase is now "proPO-A1, prophenol oxidase A1" to indicate that the CG5779 annotation encodes the structural locus of the A1 component of phenol oxidase originally described in FBrf0017315, which is produced as a proenzyme. The symbol of the annotation has also been renamed from CG5779 to CG42639 in release 5.22 of the genome annotation to try to avoid confusion. In addition, the "Phox, Phenol oxidase" gene originally described in FBrf0034818 had been merged with the proPO-A1 gene (and thus with the Black cells (Bc) mutation) in FlyBase. This is probably because in FBrf0064348 it is suggested that Phox may be the same locus as proPO-A1, since it maps to the same location and both proPO-A1 and Phox show monophenol oxidase activity. However, FBrf0036109 determined the molecular weight of Phox to be 54kD, which differs from the weight reported for proPO-A1 in FBrf0027149. Thus the "Phox, Phenol oxidase" gene originally described in FBrf0034818 has also been split out into a separate gene in its own right in FlyBase (release FB_2009_09). (Millburn, 2009.9.4) "Phox" may correspond to "proPO-A1", since they map to the same location and both show monophenol oxidase activity. (Asada et al., 1993) It is unlikely that Phox corresponds to proPO-A1 (the A1 component of phenol oxidase described in FBrf0017315). (Batterham and MacKechnie, 1980)
Other Comments
	FlyBase curator comment: the gene referred to as "Black cells" in FBrf0209379 does not correspond to the Bc mutation, but in fact corresponds to proPO-A1 (FBgn0261362). (Tokusumi et al., 2009) FlyBase curator comment: the gene referred to as "Black cells" in FBrf0207779 does not correspond to the Bc mutation, but in fact corresponds to proPO-A1 (FBgn0261362) by BLAST of the primer sequences given in the paper. The confusion probably arose due to errors in FlyBase prior to release FB_2009_09 in the representation of D.melanogaster phenol oxidase genes (see FBrf0208743). (Ye et al., 2009) FlyBase curator comment: the gene referred to as "Bc, Black cells" in FBrf0201838 does not correspond to the Bc mutation, but in fact corresponds to proPO-A1 (FBgn0261362) by BLAST of the PCR primer sequences given in the paper. The confusion probably arose due to errors in FlyBase prior to release FB_2009_09 in the representation of D.melanogaster phenol oxidase genes (see FBrf0208743). (Gajewski et al., 2007) FlyBase curator comment: the gene referred to as "Black cells" in FBrf0167944 does not correspond to the Bc mutation, but in fact corresponds to proPO-A1 (FBgn0261362) from the Bloomington stock used. The confusion probably arose due to errors in FlyBase prior to release FB_2009_09 in the representation of D.melanogaster phenol oxidase genes (see FBrf0208743). (Anholt et al., 2003) FlyBase curator comment: the gene referred to as "Bc, Black cells" in FBrf0167995 does not correspond to the Bc mutation, but in fact corresponds to proPO-A1 (FBgn0261362) by BLAST of the primer sequence given in the paper for cDNA generation. The confusion probably arose due to errors in FlyBase prior to release FB_2009_09 in the representation of D.melanogaster phenol oxidase genes (see FBrf0208743). (Fossett et al., 2003) Males have a minimum level of phenol oxidase activity that protects them from mechanical damage of cuticle or toxic effects. Females have high levels of phenol oxidase, may be caused by their reproductive function. (Bobak et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057464 RefSeq (Proteins) • NP_476812 DDBJ / EMBL / GenBank • AA816984 AAF57775 AC004640 AI107318 AI107728 BI214919 BI216824 BI241542 BI243715 BI361653 BI369509 BI482709 BI484732 BI566636 BI573407 BI573408 BI573409 BI573410 BI576652 BI579706 BI582314 BI582315 BI583721 BI584787 BI584960 BI587863 BI606027 BI607274 BI607853 BI608188 BI608657 BI610063 BI611191 BI611483 BI611613 BI611642 BI613003 BI614166 BI614485 BI614795 BI616744 BI619301 BI620556 BI623727 BI626040 BI628751 BI629114 BI631586 BI634998 CO289996 CO315875 EC070306 EC079715 EC200769 EC233060 EC244956 EC249767 GH749482 GH753329 GH754580 GH755239 GH758897 GH759118 GH770630 GH772415 GH772666 GH776791 GH781158 GH783432 GH784491 GH785680 GH790327 GH792137 GH792693 GH794147 GH794951 GH797522 GH798120 GH803196 GH804038 GH805697 GH807531 GH808324 GH812878 GH813272 GH815926 GH818156 GH819648 GH821167 GH822483 GH823324 GH828569 GH828967 GH832520 GH833030 GH835287 GH836960 GH837059 GH847043 GH848200 GH849421 GH853985 GH854171 GH854311 GH855293 GH856005 GH857700 GH857840 GH860079 GH861815 GH862764 GH865793 GH869598 GH870617 GH872962 GH874911 GH875526 GH875882 GH878203 GH879690 GH883725 GH886016 GH886907 GH887325 GH888589 GH889989 GH891203 GH891351 GH891514 GH891819 GH893133 GH893469 GH897192 GH901090 GH902360 GH904872 GH908058 GH909350 GH912246 GH916779 GH916837 GH917962 GH919464 GH919756 GH920171 GH920559 GH921254 GH923521 GH924582 GH931079 GH931185 GH932011 GH932306 GH932467 GH935706 GH940667 Entrez Gene - A searchable database of RefSeq genes. • 37044 UniProtKB/TrEMBL • Q7K2W6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Immunoglobulin E-set (IPR014756) Hemocyanin/hexamerin (IPR013788) Hemocyanin, N-terminal (IPR005204) Hemocyanin/hexamerin middle domain (IPR000896) Uncharacterised domain, di-copper centre (IPR008922) Tyrosinase (IPR002227) Hemocyanin, C-terminal (IPR005203) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH04080
Linkouts
	BioGRID - A database of protein and genetic interactions • 62729 DroID - A comprehensive database of gene and protein interactions. • FBgn0261362 DRSC - Results from RNAi screens. • FBgn0261362 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0261362 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261362 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37044 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37044 modMine - Data generated by the modENCODE project. • FBgn0261362
Synonyms & Secondary IDs ( 34 )
Reported As
Symbol Synonym	A1 (Batterham and MacKechnie, 1980) A1 (Asada and Sezaki, 1999, Asada et al., 1993, Asada, 1998) CG5779 (Reumer et al., 2009, Giot et al., 2003, Asha et al., 2003, Battersby et al., 2003, Ferjoux et al., 2007, Karlsson et al., 2004, Keleman et al., 2009.8.5, Gajewski et al., 2007, Nam et al., 2008, Wertheim et al., 2005, Seinen et al., 2010, Li et al., 2012) CG42639   DmePPOA1 (Burmester, 2001) DoxA1 (Reumer et al., 2009, Asano and Takebuchi, 2009) Dox-A1 (Belokon' et al., 1990, Bobak et al., 1991, Karlsson et al., 2004, True et al., 2001) i12 (Battersby et al., 2003) Mox (Asada, 1997, Asada et al., 1993, Asada et al., 2004, Cerenius and Soderhall, 2004, Asada et al., 2003, True et al., 2001, Sezaki et al., 2001, Aleporou et al., 2003) Phox (True et al., 2001) PO (Matskevich et al., 2010) PO54 (Bras et al., 2012) PO A1 (Omelyanchuk et al., 2001) PPO (Matskevich et al., 2010, Sinenko et al., 2010) PPO1 (Nam et al., 2008, Nam et al., 2012, Li et al., 2012, Chen et al., 2012) pro-A (Fossett et al., 2003) Propo (Grigorian et al., 2011) proPO (Matskevich et al., 2010, True et al., 2000.10.19) pro-PO (Fujimoto, 1995.2.2, Waltzer et al., 2002, Waltzer et al., 2003) proPO54 (Reumer et al., 2009, Ferjoux et al., 2007) ProPO A1 (Asano and Takebuchi, 2009) proPO-A1 (Millburn, 2009.9.4, Nawaz et al., 2012) proPO A1 (Chosa et al., 1997) pro-POA1 (Fujimoto et al., 1995, Fujimoto et al., 1995)
Name Synonym	Diphenol oxidase A1 subunit   Monophenol oxidase (Sezaki et al., 2001) Monophenoloxidase (Asada et al., 1993) phenoloxidase (Matskevich et al., 2010) Prophenoloxidase (True et al., 2001) prophenoloxidase (Sinenko et al., 2010) Prophenoloxidase A1 (Reumer et al., 2009, Evans et al., 2003) pro-phenol oxidase A1 (Fujimoto, 1995.2.2) prophenol oxidase A1 (Millburn, 2009.9.4, Paré et al., 2012) prophenoloxidase A1 (Fossett et al., 2003)
Secondary FlyBase IDs
	FBgn0000165
References ( 61 )
Generate a list of	All references relating to this gene ( 61 )
List References by type	Research paper  ( 40 ) Supplementary material  ( 4 ) Review  ( 3 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 )
Recent research papers ( 7 )
	Bras et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(13): 4986--4991 Myeloid leukemia factor is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis. [FBrf0217882] Chen et al., 2012, Dev. Comp. Immunol. 38(1): 88--97 Specific amino acids affecting Drosophila melanogaster prophenoloxidase activity in vitro. [FBrf0219007] Li et al., 2012, Dev. Comp. Immunol. 36(4): 648--656 Properties of Drosophila melanogaster prophenoloxidases expressed in Escherichia coli. [FBrf0217383] Nam et al., 2012, EMBO J. 31(5): 1253--1265 Genetic evidence of a redox-dependent systemic wound response via Hayan Protease-Phenoloxidase system in Drosophila. [FBrf0217593] Nawaz et al., 2012, PLoS ONE 7(5): e35640 Differential evolutionary wiring of the tyrosine kinase btk. [FBrf0218278] Paré et al., 2012, PLoS ONE 7(5): e36254 The functions of grainy head-like proteins in animals and fungi and the evolution of apical extracellular barriers. [FBrf0218280] Grigorian et al., 2011, Dev. Genes Evol. 221(3): 121--131 Hematopoiesis at the onset of metamorphosis: terminal differentiation and dissociation of the Drosophila lymph gland. [FBrf0214596] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...