FlyBase Gene Report: Dmel\didum

General Information

Symbol

Dmel\didum

Species

D. melanogaster

Name

dilute class unconventional myosin

Annotation Symbol

CG2146

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261397

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Snapshot

dilute class unconventional myosin (didum) encodes a class V unconventional myosin that binds actin and hydrolyzes ATP. It functions in mRNA localization in the oocyte, spermatid individualization during spermatogenesis, transport of pigment granules in photoreceptors and mitochondrial transport in neuronal cells. [Date last reviewed: 2019-02-28]

Other Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

MyoV, myosin V, shorty, DmV, MyoV43CD

Key Links

Genomic Location

Cytogenetic map

43C7-43D1

Sequence location

2R:7,500,147..7,508,625 [+]

Recombination map

2-56

RefSeq locus

NT_033778 REGION:7500147..7508625

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (25 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

enables actin binding

inferred from direct assay

(Toth et al., 2005)

enables actin-dependent ATPase activity

inferred from direct assay

(Ji et al., 2015, Toth et al., 2005)

enables ATP binding

inferred from direct assay

(Toth et al., 2005)

enables calcium-dependent ATPase activity

inferred from direct assay

(Ji et al., 2015)

enables calmodulin binding

inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253

(Martin and Bayley, 2004)

enables myosin light chain binding

inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687

(Franke et al., 2006, Toth et al., 2005)

inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253

(Toth et al., 2005)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables actin binding

inferred from sequence or structural similarity with MGI:MGI:105976

(Bonafe and Sellers, 1998, MacIver et al., 1998)

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

enables actin-dependent ATPase activity

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

enables ATP binding

inferred from sequence or structural similarity with MGI:MGI:105976

(Bonafe and Sellers, 1998, MacIver et al., 1998)

enables microfilament motor activity

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (10 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in actin filament-based movement

inferred from direct assay

(Toth et al., 2005)

involved_in anterior/posterior axis specification, embryo

inferred from high throughput mutant phenotype

(Luschnig et al., 2004)

involved_in intracellular protein transport

inferred from mutant phenotype

(Pocha et al., 2011)

inferred from genetic interaction with FLYBASE:crb; FB:FBgn0259685

(Pocha et al., 2011)

involved_in mitochondrion transport along microtubule

inferred from direct assay

(Pathak et al., 2010)

NOT involved_in oogenesis

inferred from mutant phenotype

(Mermall et al., 2005)

involved_in positive regulation of pole plasm oskar mRNA localization

inferred from mutant phenotype

(Krauss et al., 2009)

involved_in rhabdomere development

inferred from mutant phenotype

(Li et al., 2007)

involved_in sperm individualization

inferred from mutant phenotype

(Mermall et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in vesicle transport along actin filament

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of myosin V complex

inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253

(Martin and Bayley, 2004)

inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687

(Franke et al., 2006)

located_in neuronal cell body

inferred from direct assay

(Pocha et al., 2011)

located_in rhabdomere

inferred from direct assay

(Pocha et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

located_in actin cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of myosin complex

inferred from sequence or structural similarity with MGI:MGI:105976

(Bonafe and Sellers, 1998, MacIver et al., 1998)

located_in vesicle

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

MYOSIN MOTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

MYOSIN MOTORS -: Myosin motors are motor proteins that use the hydrolysis of ATP to drive movement along actin filaments. They can be divided into two groups: conventional myosins (myosin II) are involved in generating the mechanical force for muscle contraction; unconventional myosins (all other classes) are involved in processes such as cell motility, cytokinesis and trafficking. (Adapted from FBrf0134714.)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\didum for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Alternative translation stop created by use of multiphasic reading frames within coding region.

Sequence Ontology: Class of Gene

gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

didum-RA

FBtr0089745

NM_057838

6478

1792

didum-RB

FBtr0089744

NM_165536

5992

1196

didum-RC

FBtr0089743

NM_165535

6114

1800

Additional Transcript Data and Comments

Reported size (kB)

6.0 (northern blot)

(Bonafe and Sellers, 1998)

6.5 (northern blot)

(MacIver et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

didum-PA

FBpp0088686

207.3

1792

8.52

NP_477186

AAF59241

didum-PB

FBpp0088685

137.9

1196

9.45

NP_724570

AAM68903

didum-PC

FBpp0088684

208.2

1800

8.60

NP_724569

AAM68902

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

1792 (aa); 207 (kD predicted)

(Bonafe and Sellers, 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\didum using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

didum_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & adult stage

(Bonafe and Sellers, 1998)

adult stage & oogenesis

oocyte

(MacIver et al., 1998)

adult stage | male

testis

(MacIver et al., 1998)

embryonic stage 1 -- 6

organism | ubiquitous

(Bonafe and Sellers, 1998)

embryonic stage 16

embryonic/larval hindgut

(Bonafe and Sellers, 1998)

embryonic stage

(MacIver et al., 1998)

adult stage oogenesis stage S10 -- S14

nurse cell

(MacIver et al., 1998)

adult stage | female

ovary

(MacIver et al., 1998)

adult stage oogenesis stage S6 -- S8

oocyte | anterior

(MacIver et al., 1998)

Additional Descriptive Data

didum transcripts are detected in embryos and adults on northern blots. Maternally derived transcripts are uniformly distributed in embryos up to stage 6. Later in embryogenesis, high levels of didum transcript are observed in the ectodermal tissue of the hindgut.

(Bonafe and Sellers, 1998)

didum transcripts are detected in adult ovaries and testes and at low levels in embryos by northern blots. They are detected by in situ hybridization in ovaries. They are localized to the oocyte from germarial stage 2 and are confined to the anterior margin of the oocyte during the middle stages of oogenesis (6-8). In late vitellogenic stages (9-11), there is strong staining in nurse cells and a loss of anterior localization in the oocyte.

(MacIver et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of myosin V complex

inferred from physical interaction with FLYBASE:Cam; FB:FBgn0000253

(Martin and Bayley, 2004)

inferred from physical interaction with FLYBASE:Mlc-c; FB:FBgn0004687

(Franke et al., 2006)

located_in neuronal cell body

inferred from direct assay

(Pocha et al., 2011)

located_in rhabdomere

inferred from direct assay

(Pocha et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\didum in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\didum

View exonic expression by tissue for Dmel\didum

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0261397

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2146

Flygut - An atlas of the Drosophila adult midgut

FBgn0261397

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of didum

Transgenic constructs containing regulatory region of didum

Deletions and Duplications ( 4 )

Disrupted in

Df(2R)del28

(Mermall et al., 2005)

Df(2R)Drl^rv21

(Mermall et al., 2005)

Df(2R)Drl^rv22

(Mermall et al., 2005)

Df(2R)Exel6052

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of embryonic stage | germline clone | maternal effect | recessive

didum¹⁵⁴

lethal - all die before end of embryonic stage | germline clone | non-rescuable maternal effect | recessive

lethal - all die before end of larval stage | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

didum^GD1848

viable, with Scer\GAL4^pnr-MD237

didum^GD1848

viable, with Scer\GAL4^tin.CΔ4

didum^GD1848

Sterility

Allele

female fertile

didum^Q1052st

female fertile | germline clone

didum^Q1052st

male sterile | recessive

didum^Q1052st

Other Phenotypes

Allele

developmental rate defective

didum^unspecified

developmental rate defective | recessive

didum^Q1052st

some die during embryonic stage | maternal effect | recessive

didum¹⁵⁴

some die during embryonic stage | non-rescuable maternal effect | recessive

some die during larval stage | recessive

didum^unspecified

some die during third instar larval stage | recessive

didum^Q1052st

Phenotype manifest in

Allele

embryo | germline clone | maternal effect | posterior

embryonic abdomen | germline clone | maternal effect

didum¹⁵⁴

embryonic head | germline clone | maternal effect

eye photoreceptor cell | somatic clone

didum^Q1052st

filzkorper | germline clone | maternal effect

male genitalia, with Scer\GAL4^Abd-B-LDN

didum^GD1848

male genitalia, with Scer\GAL4^tsh-md621

didum^GD1848

ovary | germline clone | maternal effect

primordial germ cell | germline clone | maternal effect

rhabdomere | ectopic

didum^KG04384

rhabdomere | ectopic | somatic clone

didum^Q1052st

rhabdomere | somatic clone

didum^Q1052st

spermatid cyst

didum^Q1052st

spermatozoon

didum^Q1052st

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (24)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MYO5A

12 of 15

Yes

Hsap\MYO5B

11 of 15

Yes

Hsap\MYO5C

10 of 15

Yes

Hsap\MYH10

3 of 15

Hsap\MYO19

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Yes

Hsap\MYO1G

1 of 15

Hsap\MYO15A

1 of 15

Hsap\RADIL

1 of 15

Yes

Hsap\RASIP1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (25)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Myo5a

11 of 15

Yes

Mmus\Myo5b

11 of 15

Yes

Mmus\Myo5c

10 of 15

Yes

Mmus\Myh10

3 of 15

Mmus\Myo19

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Radil

1 of 15

Yes

Mmus\Rasip1

1 of 15

Yes

Rattus norvegicus (Norway rat) (24)

Rnor\Myo5b

9 of 13

Yes

Rnor\Myo5c

9 of 13

Yes

Rnor\Myo5a

8 of 13

Yes

Rnor\Myo19

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Rnor\LOC103693367

1 of 13

Yes

Rnor\Myh8

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Rasip1

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (30)

Xtro\myo5a

7 of 12

Yes

Xtro\myo5b

6 of 12

Yes

Xtro\myo19

3 of 12

Yes

Xtro\myh6

2 of 12

Xtro\myo5c

2 of 12

Yes

Xtro\iqcb1

1 of 12

Yes

Xtro\LOC100485637

1 of 12

Xtro\LOC100485792

1 of 12

Yes

Xtro\LOC100493121

1 of 12

Yes

Xtro\LOC100493774

1 of 12

Xtro\LOC100495065

1 of 12

Yes

Xtro\LOC100495212

1 of 12

Yes

Xtro\LOC100495638

1 of 12

Yes

Xtro\LOC100495802

1 of 12

Yes

Xtro\LOC100495968

1 of 12

Yes

Xtro\LOC101733223

1 of 12

Yes

Xtro\LOC101734799

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\myh7

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Danio rerio (Zebrafish) (41)

Drer\myo5b

9 of 15

Yes

Drer\myo5aa

8 of 15

Yes

Drer\myo5ab

8 of 15

Yes

Drer\myo5c

7 of 15

Yes

Drer\si:dkey-110c1.10

7 of 15

Yes

Drer\myo19

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\rasip1

1 of 15

Yes

Drer\smyhc3

1 of 15

Drer\wu:fd14a01

1 of 15

Drer\zgc:66156

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\hum-2

14 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Yes

Cele\unc-15

1 of 15

Arabidopsis thaliana (thale-cress) (17)

Atha\MYA1

7 of 9

Yes

Atha\XIC

7 of 9

Yes

Atha\XIE

7 of 9

Yes

Atha\XI-I

7 of 9

Yes

Atha\XIJ

7 of 9

Yes

Atha\MYA2

6 of 9

Yes

Atha\XIF

6 of 9

Yes

Atha\XIK

6 of 9

Yes

Atha\VIIIA

5 of 9

Yes

Atha\XIA

5 of 9

Yes

Atha\XIB

5 of 9

Yes

Atha\XID

5 of 9

Yes

Atha\XIG

5 of 9

Yes

Atha\XIH

5 of 9

Yes

Atha\ATM1

4 of 9

Yes

Atha\ATM2

3 of 9

Yes

Atha\VIIIB

3 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\MYO2

14 of 15

Yes

Scer\MYO4

9 of 15

Yes

Scer\MYO1

2 of 15

Scer\YPR089W

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\myo52

10 of 12

Yes

Spom\myo51

8 of 12

Yes

Spom\myo2

2 of 12

Spom\myp2

2 of 12

Spom\SPAC25B8.08

1 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900CG )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15267

Drosophila persimilis

Dper\GL20011

Drosophila willistoni

Dwil\GK10684

Drosophila virilis

Dvir\GJ22297

Drosophila mojavensis

Dmoj\GI19162

Drosophila grimshawi

Dgri\GH22080

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150070 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17021447

Glossina morsitans

Tsetse fly

Gmor\GMOY008690

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13172

Mayetiola destructor

Hessian fly

Mdes\Mdes010761

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009357

Anopheles darlingi

American malaria mosquito

Adar\ADAC004331

Anopheles gambiae

Malaria mosquito

Agam\AGAP006479

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009300

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00HU )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA009779

Danaus plexippus

Monarch butterfly

Dple\DPOGS212512-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL009324

Apis florea

Little honeybee

Aflo\2864764676

Apis mellifera

Western honey bee

Amel\GB50690

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP23617

Bombus terrestris

Buff-tailed bumblebee

Bter\15210228

Linepithema humile

Argentine ant

Lhum\LH11771

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002ee6

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006416

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05340

Tribolium castaneum

Red flour beetle

Tcas\TC009935

Pediculus humanus

Human body louse

Phum\PHUM047290

Rhodnius prolixus

Kissing bug

Rpro\RPRC013067

Cimex lectularius

Bed bug

Clec\CLEC004169

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005774

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI26900

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI49226

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_07190

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00H0 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR013439

Ixodes scapularis

Black-legged tick

Isca\ISCW021017

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70740.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70741.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur06g00630

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_308269

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_100958

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0G93 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-MyV

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-My5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-MyosinV

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-MyV_1

Ciona intestinalis

Vase tunicate

Cint\LOC100186725

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000012984

Gallus gallus

Domestic chicken

Ggal\MYO5C

Gallus gallus

Domestic chicken

Ggal\MYO5A

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (15)

Gene Symbol

Score

Source

Alignment

4 of 10

Mhc

3 of 10

Myo10A

3 of 10

zip

3 of 10

2 of 10

jar

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

Prm

2 of 10

CG31638

1 of 10

CG43796

1 of 10

Mhcl

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

MYO5A; myosin VA

model of Griscelli syndrome type 1

IEA

(FlyBase, 2019-)

MYO5B; myosin VB

model of microvillus inclusion disease

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MYO5A; myosin VA

12 of 15

160777

GRISCELLI SYNDROME, TYPE 1; GS1

Griscelli syndrome type 1

MYO5B; myosin VB

11 of 15

606540

DIARRHEA 2, WITH MICROVILLUS ATROPHY; DIAR2

microvillus inclusion disease

MYO5C; myosin VC

10 of 15

610022

MYH10; myosin heavy chain 10

3 of 15

160776

MYO19; myosin XIX

3 of 15

617379

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

didum - Act57B

experimental knowledge based

(Guruharsha et al., 2011)

didum - Arp2

experimental knowledge based

(Guruharsha et al., 2011)

didum - Arpc2

experimental knowledge based

(Guruharsha et al., 2011)

didum - CG7611

pull down, anti tag western blot

(Finan et al., 2011)

didum - Cam

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Satoh et al., 2008, Toth et al., 2005)

didum - Kaz

pull down, anti tag western blot

(Finan et al., 2011)

didum - Khc

two hybrid

(Krauss et al., 2009)

didum - Mer

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

didum - Mlc-c

anti tag coimmunoprecipitation, western blot, Identification by mass spectrometry, peptide massfingerprinting, anti bait coimmunoprecipitation

(Satoh et al., 2008, Franke et al., 2006, Toth et al., 2005)

didum - Ntan1

experimental knowledge based

(Guruharsha et al., 2011)

didum - Nup93-1

pull down, anti tag western blot

(Finan et al., 2011)

didum - Patj

anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(Pocha et al., 2011)

didum - Rab11

pull down, peptide massfingerprinting, anti tag coimmunoprecipitation, autoradiography, anti tag western blot, two hybrid

(Ji et al., 2015, Ramel et al., 2013, Satoh et al., 2008, Li et al., 2007)

didum - Rab32

pull down, anti tag western blot, anti tag coimmunoprecipitation, autoradiography, two hybrid

(Ji et al., 2015, Satoh et al., 2008)

didum - SMC5

anti tag coimmunoprecipitation, anti tag western blot

(Caridi et al., 2018)

didum - Swip-1

experimental knowledge based

(Guruharsha et al., 2011)

didum - cpa

experimental knowledge based

(Guruharsha et al., 2011)

didum - cpb

experimental knowledge based

(Guruharsha et al., 2011)

didum - jar

anti bait coimmunoprecipitation, western blot, pull down, peptide massfingerprinting

(Finan et al., 2011)

didum - jnj

anti tag coimmunoprecipitation, anti tag western blot

(Caridi et al., 2018)

didum - osk

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, Identification by mass spectrometry

(Hurd et al., 2016, Krauss et al., 2009)

didum - sdt

anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(Pocha et al., 2011)

didum - stau

anti tag coimmunoprecipitation, western blot

(Lu et al., 2018)

RNA-protein

Physical Interaction

Assay

References

didum - osk

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, Identification by mass spectrometry

(Hurd et al., 2016, Krauss et al., 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

fzr

suppressible

didum

(Neuert et al., 2017)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61556

DroID - A comprehensive database of gene and protein interactions.

FBgn0261397

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35680

MIST (protein-protein) - An integrated Molecular Interaction Database

didum

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-56

Cytogenetic map

43C7-43D1

Sequence location

2R:7,500,147..7,508,625 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43C7-43D1

Limits computationally determined from genome sequence between P{lacW}cos^k16101 and P{EP}CG1600^EP398

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

43B-43B

(Maciver, 1996.9.17)

43C-43D

(Bonafe, 1997.5.12)

43B-43C

(determined by in situ hybridisation)

(MacIver et al., 1998, McCormack et al., 1996)

43C-43D

(determined by in situ hybridisation)

(Bonafe and Sellers, 1998)

Experimentally Determined Recombination Data

Location

2-56

(FlyBase, 2016)

2-56.6

(Comeron et al., 2012)

(Luschnig et al., 2004, McCormack et al., 1996)

Left of (cM)

(Krauss et al., 2009)

Right of (cM)

Notes

Maps to 2R.

(Luschnig et al., 2004)

Stocks and Reagents

Stocks (8)

Bloomington

14094

y¹; P{SUPor-P}didum^KG04384/CyO

76986

y¹ sc^* v¹ sev²¹; P{TKO.GS00739}attP40

78199

y¹ sc^* v¹ sev²¹; P{TOE.GS01374}attP40

55740

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03900}attP40

VDRC

v16902

w¹¹¹⁸; P{GD1848}v16902

v44291

w¹¹¹⁸; P{GD1848}v44291

v44292

w¹¹¹⁸; P{GD1848}v44292

v318855

PBac{fTRG01350.sfGFP-TVPTBF}VK00033

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (58)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE30195

Other clones

FI23904

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261397

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261397

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35680

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lu et al., 2018, Finan et al., 2011, Krauss et al., 2009, Toth et al., 2005)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: didum CG2146

(Gabrielian, 1999.11)

Source for database merge of

Source for merge of: soy didum

(Krauss et al., 2009)

Source for merge of: didum NEST:bs14c05

(FlyBase, 1996-)

Additional comments

soy mutants complement tud and stau mutants.

(Luschnig et al., 2004)

"l(2)43Cc" may correspond to "didum"; there is a band shift in the didum genomic region associated with the "l(2)43Cc⁴" mutation which is not a polymorphism, although it maps several kilobases 5' of the cloned genomic didum sequence.

(MacIver et al., 1998)

Source for merge of didum NEST:bs14c05 was sequence comparison ( date:031022 ).

(FlyBase, 1996-)

Other Comments

didum is required for wls protein-containing exosome release at synaptic boutons.

(Koles et al., 2012)

didum knockdown increases axonal mitochondrial length. didum acts to interrupt mitochondrial transport on microtubules to decrease the duty cycle and perhaps facilitate docking.

(Pathak et al., 2010)

didum protein associates with the osk mRNA transport complex preferentially at the oocyte cortex.

(Krauss et al., 2009)

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

(Inlow and Restifo, 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

Area matching Drosophila myosin V gene (inverted), Acc. No. AF003826.

(Davies, 2001.3.15)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35680

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261397

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

didum_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2146

Flygut - An atlas of the Drosophila adult midgut

FBgn0261397

FlyMine - An integrated database for Drosophila genomics

FBgn0261397

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35680

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009935

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2146

modMine - A data warehouse for the modENCODE project

FBgn0261397

Linkouts

BioGRID - A database of protein and genetic interactions.

61556

DroID - A comprehensive database of gene and protein interactions.

FBgn0261397

DRSC - Results frm RNAi screens

FBgn0261397

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0261397

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35680

MIST (protein-protein) - An integrated Molecular Interaction Database

didum

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

43CD

(Oliver et al., 1999)

CG2146

(Laffafian and Tepass, 2019, Kolkhof et al., 2017, Neuert et al., 2017, Çiçek et al., 2016, Harris et al., 2016, Hurd et al., 2016, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Krahmer et al., 2013, Ramel et al., 2013, Petzoldt et al., 2012, Ni et al., 2010.12.1, Perkins et al., 2009, Rogers et al., 2003, Goldstein and Gunawardena, 2000)

Didum

(Miettinen and Björklund, 2015, Ramel et al., 2013, Zhang et al., 2013, Liebl and Featherstone, 2008, Bandyopadhyay et al., 2006)

DmV

(Toth et al., 2005, Yamashita et al., 2000)

(Finan et al., 2011)

MYOV

(Goldstein and Gunawardena, 2000)

Myo1C

(Kollmar, 2015.9.15)

Myo5

(Koles et al., 2012)

MyoV

(Aguilar-Aragon et al., 2020, Lattner et al., 2019, Caridi et al., 2018, Pichaud, 2018, Harris et al., 2016, Hurd et al., 2016, Iwanami et al., 2016, Roman-Fernandez and Bryant, 2016, Xiong and Bellen, 2013, Gault et al., 2012, Pocha et al., 2011, Windler and Bilder, 2010, Krauss et al., 2009, Satoh et al., 2008, Li et al., 2007, Mermall et al., 2005, Yamashita et al., 2000)

MyoV43CD

(Bonafe and Sellers, 1998)

NEST:bs14c05

NMC7

(MacIver et al., 1998)

dmM5

(Ji et al., 2015)

myoV

(Massarwa et al., 2009)

soy

Name Synonyms

Didum

(Wong et al., 2011)

Myosin V

(Schopf and Huber, 2017, Krahmer et al., 2013, Zoller and Schulz, 2012, Sun et al., 2011, Serio et al., 2010, Satoh et al., 2008, Rogers et al., 2003)

Myosin5

(Laffafian and Tepass, 2019)

MyosinV

(Shah et al., 2004)

dilute class unconventional myosin

(Rudrapatna et al., 2014, Wang and Lin, 2007, Nelson et al., 2005)

myosin V

(Couderc et al., 2017, Finan et al., 2011, Pathak et al., 2010, Nagy et al., 2009, Toth et al., 2005, Welte, 2004, Rogers et al., 2003, Bonafe et al., 2002, Goldstein and Gunawardena, 2000, Bonafe et al., 1999, Bonafe and Sellers, 1998, McCormack et al., 1998, Bonafe, 1997.5.12, Bonafe and Sellers, 1997)

myosin-5

(Ji et al., 2015)

myosin-V

(Bonafe et al., 1996)

shorty

(Krauss et al., 2009, Krauss et al., 2009, Luschnig et al., 2004, Luschnig et al., 2004)

Secondary FlyBase IDs

FBgn0069080
FBgn0015933
FBgn0023490
FBgn0045305

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (135)

The following external sites may use different assemblies or annotations than FlyBase.

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