FB2019_05, released Oct 15, 2019

Gene: Dmel\RhoGAP18B

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General Information
Symbol
Dmel\RhoGAP18B
Species
D. melanogaster
Name
Rho GTPase activating protein at 18B
Annotation Symbol
CG42274
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261461
Gene Model Status
Current
Stock Availability
48 publicly available
Gene Snapshot
Rho GTPase activating protein at 18B (RhoGAP18B) encodes a protein that can act as a GTPase activating protein for several Rho GTPase and is involved in the regulation of actin dynamics. [Date last reviewed: 2019-08-01]
Other Summaries
Also Known As
whir, white rabbit
Key Links
Genomic Location
Cytogenetic map
18A3-18A4
Sequence location
X:19,136,107..19,167,082 [-]
Recombination map
1-62
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
GTPase activator activity; Rho GTPase binding
Predictions / Assertions
-
Summaries
Gene Group (FlyBase)
RHO GTPASE ACTIVATING PROTEINS -
RhoGAPs are GTPase activating proteins for Rho family GTPases. (Adapted from PMID:15731001 and FBrf0158741).
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\RhoGAP18B or the JBrowse view of Dmel\RhoGAP18B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.55
Gene model reviewed during 5.42
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Transposon in 3' UTR.
Gene model reviewed during 5.49
Gene model reviewed during 5.56
Sequence Ontology: Class of Gene
gene
gene_with_edited_transcript
(Rodriguez et al., 2012)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
RhoGAP18B-RB
FBtr0299622
NM_133123
2208
468
RhoGAP18B-RC
FBtr0299623
NM_133124
4695
1317
RhoGAP18B-RD
FBtr0299624
NM_001144748
3931
752
RhoGAP18B-RF
FBtr0299625
NM_001144749
1689
468
RhoGAP18B-RH
FBtr0339261
NM_001298497
1787
468
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
RhoGAP18B-PB
FBpp0288897
52.2
468
8.27
NP_573351
AAF48919
RhoGAP18B-PC
FBpp0288898
147.3
1317
6.87
NP_573352
AAF48921
RhoGAP18B-PD
FBpp0288899
84.5
752
4.85
NP_001138220
ACL82950
RhoGAP18B-PF
FBpp0288900
52.2
468
8.27
NP_001138221
ACL82951
RhoGAP18B-PH
FBpp0308368
52.2
468
8.27
NP_001285426
AHN59896
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

468 aa isoforms: RhoGAP18B-PB, RhoGAP18B-PF, RhoGAP18B-PH
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RhoGAP18B using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (7 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
GTPase activator activity
inferred from direct assay
(Rothenfluh et al., 2006)
Rho GTPase binding
inferred from physical interaction with FLYBASE:Rac1; FB:FBgn0010333
(Ojelade et al., 2015)
inferred from physical interaction with FLYBASE:Cdc42; FB:FBgn0010341
(Ojelade et al., 2015)
inferred from physical interaction with FLYBASE:Rho1; FB:FBgn0014020
(Ojelade et al., 2015)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
actin cytoskeleton organization
inferred from mutant phenotype
(Ojelade et al., 2015)
positive regulation of GTPase activity
inferred from direct assay
(Rothenfluh et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
regulation of GTPase activity
inferred from biological aspect of ancestor with PANTHER:PTN000571279
(assigned by GO_Central )
(Gaudet et al., 2011)
signal transduction
inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000571279
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 11 -- 12
dorsal epidermis primordium
(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic head epidermis
(Fisher et al., 2012)
embryonic ventral epidermis
(Fisher et al., 2012)
prepupal stage P3
leg | ubiquitous
(Greenberg and Hatini, 2011)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head

Comment: isoform RC

(Rothenfluh et al., 2006)
organism

Comment: isoform RA

(Rothenfluh et al., 2006)
organism | intense

Comment: isoform RD

(Rothenfluh et al., 2006)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head

Comment: isoforms RA, RC and RD

(Rothenfluh et al., 2006)
Additional Descriptive Data
Expression in stage P3 pupal leg discs is ubiquitous.
(Greenberg and Hatini, 2011)
RhoGAP18B isoform RD is detected in both adult head and body whereas isoform RA is just detected in the body and isoform RC predominantly in the adult head.
(Rothenfluh et al., 2006)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RhoGAP18B in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 58 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of RhoGAP18B
Transgenic constructs containing regulatory region of RhoGAP18B
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\ARHGAP9
2 of 15
Yes
Yes
Hsap\ARHGAP12
2 of 15
Yes
Yes
Hsap\ARHGAP15
2 of 15
Yes
No
Hsap\ARHGAP27
2 of 15
Yes
Yes
Hsap\RACGAP1
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Arhgap9
2 of 15
Yes
Yes
Mmus\Arhgap12
2 of 15
Yes
Yes
Mmus\Arhgap15
2 of 15
Yes
No
Mmus\Arhgap27
2 of 15
Yes
Yes
Mmus\Racgap1
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
Rnor\Arhgap9
2 of 13
Yes
Yes
Rnor\Arhgap15
2 of 13
Yes
Yes
Rnor\Arhgap27
2 of 13
Yes
Yes
Rnor\Arhgap12
1 of 13
No
No
Rnor\Racgap1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\arhgap12
1 of 12
Yes
Yes
Xtro\arhgap27
1 of 12
Yes
Yes
Xtro\racgap1
1 of 12
Yes
No
Danio rerio (Zebrafish) (8)
Drer\arhgap9
2 of 15
Yes
No
Drer\arhgap12a
2 of 15
Yes
Yes
Drer\arhgap12b
2 of 15
Yes
Yes
Drer\arhgap15
2 of 15
Yes
Yes
Drer\arhgap27l
2 of 15
Yes
Yes
Drer\arhgap27
1 of 15
No
No
Drer\racgap1
1 of 15
No
No
Drer\si:ch1073-416j23.1
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\pac-1
1 of 15
Yes
No
Cele\srgp-1
1 of 15
Yes
Yes
Cele\tag-325
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (1)
Atha\AT5G19390
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\BEM2
2 of 15
Yes
No
Scer\BEM3
1 of 15
No
Yes
Scer\RGA1
1 of 15
No
No
Scer\RGA2
1 of 15
No
No
Scer\RGD1
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (4)
Spom\rga2
2 of 12
Yes
Yes
Spom\rga3
1 of 12
No
No
Spom\rga4
1 of 12
No
No
Spom\rga7
1 of 12
No
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919047J )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\RhoGAP18B
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00004804
Drosophila simulans
Dsim\GD15602
Drosophila sechellia
Dsec\GM22767
Drosophila sechellia
Dsec\GM22768
Drosophila erecta
Dere\GG18077
Drosophila yakuba
Dyak\GE15475
Drosophila ananassae
Dana\GF16007
Drosophila pseudoobscura pseudoobscura
Dpse\GA22300
Drosophila pseudoobscura pseudoobscura
Dpse\GA31963
Drosophila persimilis
Dper\GL14655
Drosophila willistoni
Dwil\GK25096
Drosophila virilis
Dvir\GJ15255
Drosophila mojavensis
Dmoj\GI15727
Drosophila grimshawi
Dgri\GH23601
Drosophila grimshawi
Dgri\GH24224
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X022Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_54327
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (8)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
RacGAP84C
2 of 10
tum
2 of 10
CdGAPr
1 of 10
RhoGAP1A
1 of 10
RhoGAP16F
1 of 10
RhoGAP19D
1 of 10
RhoGAP100F
1 of 10
Rlip
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement
View in Interactions Browser
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
RhoGAP18B
suppressible
Arf51F
(Gonzalez et al., 2018, Peru Y Colón de Portugal et al., 2012)
RhoGAP18B
suppressible
Arfip
(Gonzalez et al., 2018, Peru Y Colón de Portugal et al., 2012)
RhoGAP18B
suppressible
Efa6
(Gonzalez et al., 2018)
RhoGAP18B
suppressible
LIMK1
(Ojelade et al., 2015)
RhoGAP18B
suppressible
Mtl|Rac1|Rac2
(Rothenfluh et al., 2006)
RhoGAP18B
suppressible
Rho1
(Rothenfluh et al., 2006)
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map
1-62
Cytogenetic map
18A3-18A4
Sequence location
X:19,136,107..19,167,082 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
18A3-18A4
Limits computationally determined from genome sequence between P{EP}CG7326EP1133 and P{EP}CG7502EP1326
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
1-62
(FlyBase, 2016)
1-60.4
(Comeron et al., 2012)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (48)
Genomic Clones (27)
cDNA Clones (132)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: whir 5-131
    (Rothenfluh, 2004.2.23)
    Source for database merge of
    Source for merge of: RhoGAP18B CG7502
    (FlyBase Genome Annotators, 2008)
    Source for merge of: RhoGAP18B whir
    (Rothenfluh et al., 2006)
    Source for merge of: CG7481 CG7531
    (FlyBase, 1996-)
    Source for merge of: RhoGAP18B CG17015
    (FlyBase Genome Annotators, 2002-2003)
    Additional comments
    Annotations CG7481 and CG7502 merged as CG42274 in release 5.8 of the genome annotation.
    (FlyBase Genome Annotators, 2008)
    Annotations CG7481, CG17015 and CG7531 merged as CG7481 in release 3 of the genome annotation.
    (FlyBase Genome Annotators, 2002-2003)
    Source for merge of CG7481 CG7531 was a shared cDNA ( date:010720 ).
    (FlyBase, 1996-)
    Other Comments
    Different isoforms of RhoGAP18B act via inhibiting distinct Rho-family GTPases to regulate actin dynamics.
    (Ojelade et al., 2015)
    RhoGAP18B is transcribed from at least two promoters separated by approximately 11kb.
    (Rothenfluh et al., 2006)
    RhoGAP18B mutants exhibit strong resistance to the sedating effects of ethanol.
    (Rothenfluh et al., 2006)
    Mutants show resistance to the behavioral effects of nicotine and altered responses to ethanol.
    (Rothenfluh et al., 2004)
    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
    (Kiger et al., 2003)
    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: increased or polarized (uneven) accumulation of F-actin.
    (Kiger et al., 2003)
    Mutants are resistant to the behavioral effects of both nicotine and ethanol. Mutants show a strongly accentuated and prolonged startle response, suggesting that they are more sensitive to the sensory input of the alcohol smell, or more responsive to it.
    (Rothenfluh and Heberlein, 2003)
    Origin and Etymology
    Discoverer
    Etymology
    Named 'white rabbit' for its diverse role in regulating responses to abused drugs as described in the song 'White Rabbit' by Jefferson Airplane.
    (Rothenfluh et al., 2006)
    Identification
    External Crossreferences and Linkouts ( 69 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (11)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (70)