FB2021_06, released December 14, 2021

Gene: Dmel\haf

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General Information
Symbol
Dmel\haf
Species
D. melanogaster
Name
hattifattener
Annotation Symbol
CG14351
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261509
Gene Model Status
Current
Stock Availability
13 publicly available
Gene Summary
hattifattener (haf) encodes a large transmembrane leucine-rich repeat (LRR) protein whose mutants have variable motor axon defects in which ISNb motor axons fail to make the correct decisions at choice points. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
22A3-22A3
Sequence location
Recombination map
2-3
RefSeq locus
NT_033779 REGION:1555129..1609389
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (1 term)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in motor neuron axon guidance
inferred from mutant phenotype
(Kurusu et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
hattifattener (haf) encodes a large transmembrane leucine-rich repeat (LRR) protein whose mutants have variable motor axon defects in which ISNb motor axons fail to make the correct decisions at choice points. [Date last reviewed: 2019-03-07]
Gene Model and Products
Number of Transcripts
8
Number of Unique Polypeptides
6

Please see the JBrowse view of Dmel\haf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.42

Gene model reviewed during 5.53

Stop-codon suppression (UGA) postulated; FBrf0234051. Double and triple stop-codon suppression (UGA, UGA, UGA) postulated by FlyBase.

Gene model reviewed during 6.25

Sequence Ontology: Class of Gene
gene_with_stop_codon_read_through
(Jungreis et al., 2016)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
haf-RB
FBtr0310017
NM_001272944
5009
981
haf-RC
FBtr0310018
NM_134763
5305
982
haf-RD
FBtr0310019
NM_001272945
6000
981
haf-RE
FBtr0310020
NM_001272946
6069
982
haf-RF
FBtr0344146
NM_001298635
4915
852
haf-RG
FBtr0474152
NM_001369974
5009
988
haf-RH
FBtr0474153
NM_001369975
5009
1001
haf-RI
FBtr0474154
NM_001369976
5009
1347
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
haf-PB
FBpp0301720
104.2
981
9.52
NP_001259873
AGB92410
haf-PC
FBpp0301721
104.3
982
9.52
NP_608607
AAF51357
haf-PD
FBpp0301722
104.2
981
9.52
NP_001259874
AGB92411
haf-PE
FBpp0301723
104.3
982
9.52
NP_001259875
AGB92412
haf-PF
FBpp0310559
91.2
852
8.61
NP_001285564
AHN54079
haf-PG
FBpp0423151
104.9
988
9.60
QCD25251
haf-PH
FBpp0423152
106.4
1001
9.50
QCD25252
haf-PI
FBpp0423153
143.9
1347
8.32
QCD25253
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

981 aa isoforms: haf-PB, haf-PD
982 aa isoforms: haf-PC, haf-PE
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\haf using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 17
embryonic/larval central nervous system
(Kurusu et al., 2008)
embryonic/larval somatic muscle cell | restricted
(Kurusu et al., 2008)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
Reporter: P{GawB}hafNP2012
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 16
embryonic/larval somatic muscle cell | restricted
(Kurusu et al., 2008)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\haf in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of haf
Transgenic constructs containing regulatory region of haf
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(2L)Exel6005
(Ryder, 2004.4.26)
Gene partially disrupted in
In(2LR)SM1
(Miller et al., 2018)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4pnr-MD237
lethal - all die during embryonic stage, with Scer\GAL4αTub84B.PL
partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal body color, with Scer\GAL4pnr-MD237
abnormal neuroanatomy, with Scer\GAL4G14
abnormal neuroanatomy, with Scer\GAL4how-24B
abnormal neuroanatomy, with Scer\GAL4l(3)H94-H94
abnormal neuroanatomy, with Scer\GAL4tey-5053A
abnormal neuroanatomy, with Scer\GAL4αTub84B.PL
abnormal neuroanatomy | recessive
abnormal pain response | adult stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
some die during embryonic stage, with Scer\GAL4αTub84B.PL
Phenotype manifest in
Allele
embryonic/larval salivary gland, with Scer\GAL4fkh.PH
larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4G14
larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4how-24B
larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4l(3)H94-H94
larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4tey-5053A
larval intersegmental nerve branch ISNb of A1-7 | embryonic stage 17
larval intersegmental nerve branch ISNb of A1-7 | embryonic stage, with Scer\GAL4αTub84B.PL
larval ventral nerve cord, with Scer\GAL4elav.PLu
mesothoracic tergum, with Scer\GAL4pnr-MD237
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\TRIL
2 of 15
Yes
Yes
Hsap\ISLR2
1 of 15
No
No
Hsap\ISLR
1 of 15
No
Yes
Hsap\LRIG1
1 of 15
No
No
Hsap\LRIG2
1 of 15
No
No
Hsap\LRIG3
1 of 15
No
No
Hsap\VASN
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Islr2
1 of 15
Yes
No
Mmus\Islr
1 of 15
Yes
Yes
Mmus\Lrig1
1 of 15
Yes
No
Mmus\Lrig2
1 of 15
Yes
No
Mmus\Lrig3
1 of 15
Yes
No
Mmus\Lrrn4
1 of 15
Yes
No
Mmus\Vasn
1 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (7)
Rnor\Islr2
1 of 13
Yes
Yes
Rnor\Islr
1 of 13
Yes
Yes
Rnor\Lrig1
1 of 13
Yes
No
Rnor\Lrig2
1 of 13
Yes
No
Rnor\Lrig3
1 of 13
Yes
No
Rnor\Lrrn4
1 of 13
Yes
Yes
Rnor\Vasn
1 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\lrig1
1 of 12
Yes
No
Xtro\lrig2
1 of 12
Yes
No
Xtro\lrig3
1 of 12
Yes
No
Xtro\vasn
1 of 12
Yes
Yes
Danio rerio (Zebrafish) (7)
Drer\islr2
1 of 15
Yes
Yes
Drer\lrig1
1 of 15
Yes
No
Drer\lrig2
1 of 15
Yes
No
Drer\si:ch211-106k21.5
1 of 15
Yes
Yes
Drer\vasna
1 of 15
Yes
Yes
Drer\vasnb
1 of 15
Yes
Yes
Drer\waif2
1 of 15
Yes
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\iglr-1
1 of 15
Yes
No
Cele\lron-2
1 of 15
Yes
No
Cele\lron-6
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902ZN )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000756
Drosophila simulans
Dsim\GD23104
Drosophila sechellia
Dsec\GM16824
Drosophila erecta
Dere\GG24798
Drosophila yakuba
Dyak\GE17637
Drosophila ananassae
Dana\GF15849
Drosophila pseudoobscura pseudoobscura
Dpse\GA12925
Drosophila persimilis
Dper\GL15753
Drosophila willistoni
Dwil\GK15199
Drosophila virilis
Dvir\GJ17086
Drosophila mojavensis
Dmoj\GI14569
Drosophila grimshawi
Dgri\GH22465
Drosophila grimshawi
Dgri\GH11418
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501BE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17022896
Glossina morsitans
Tsetse fly
Gmor\GMOY006649
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_06719
Mayetiola destructor
Hessian fly
Mdes\Mdes015126
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL010286
Anopheles darlingi
American malaria mosquito
Adar\ADAC010414
Anopheles gambiae
Malaria mosquito
Agam\AGAP007846
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ003844
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02PJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA003122
Danaus plexippus
Monarch butterfly
Dple\DPOGS201338-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL012398
Apis florea
Little honeybee
Aflo\2987438532
Apis mellifera
Western honey bee
Amel\GB45364
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP24572
Bombus terrestris
Buff-tailed bumblebee
Bter\15212030
Linepithema humile
Argentine ant
Lhum\LH23759
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000750
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG003756
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_06214
Tribolium castaneum
Red flour beetle
Tcas\TC014301
Pediculus humanus
Human body louse
Phum\PHUM375610
Rhodnius prolixus
Kissing bug
Rpro\RPRC012446
Cimex lectularius
Bed bug
Clec\CLEC012987
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006523
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_02888
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02MT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR012274
Strigamia maritima
European centipede
Smar\SMAR008884
Ixodes scapularis
Black-legged tick
Isca\ISCW020146
Stegodyphus mimosarum
African social velvet spider
Smim\KFM63920.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur10g04730
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0416 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Tlr016
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (13)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG7896
1 of 10
CG16974
1 of 10
CG18249
1 of 10
CG18480
1 of 10
hfw
1 of 10
kek1
1 of 10
kek2
1 of 10
kek3
1 of 10
kek6
1 of 10
Lapsyn
1 of 10
Lrt
1 of 10
rdo
1 of 10
swi2
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    hafEY11244
    exacerbates  tauopathy
    modeled by Hsap\MAPTUAS.cWa
    (Feuillette et al., 2020)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-3
    Cytogenetic map
    22A3-22A3
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    22A3-22A3
    Limits computationally determined from genome sequence between P{PZ}l(2)1068510685&P{lacW}Tango14k00619 and P{lacW}RFeSPk11704
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-3

    (FlyBase, 2016)

    2-2.7

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (13)
    Genomic Clones (45)
    cDNA Clones (33)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Kurusu et al., 2008)

    monoclonal

    (Kurusu et al., 2008)
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: haf CG14351

    (Kurusu et al., 2008)
    Additional comments
    Other Comments
    Origin and Etymology
    Discoverer
    Etymology

    Hattifatteners are worm-like creatures from the Moomin stories that float randomly around the world in little boats.

    (Kurusu et al., 2008)
    Identification
    External Crossreferences and Linkouts ( 51 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL - MARRVEL (model organism gene)
    modMine - A data warehouse for the modENCODE project
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Synonyms and Secondary IDs (5)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (48)