FlyBase Gene Report: Dmel\Orp8

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Orp8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fat body

Comment: cyclical, peak ZT1-4

(Xu et al., 2011)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval midgut

(Hernández de Madrid and Casanova, 2018)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

visceral muscle primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval visceral muscle cell

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic proventriculus

(Fisher et al., 2012)

embryonic/larval midgut interstitial cell

(Fisher et al., 2012)

Additional Descriptive Data

Expressed cyclically in the adult fat body.

(Xu et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Orp8 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Orp8

View exonic expression by tissue for Dmel\Orp8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261550

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42412/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261550

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261550

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5077

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Orp8

Transgenic constructs containing regulatory region of Orp8

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6185

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Orp8^GD476

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Orp8^GD476

Phenotype manifest in

Allele

abdominal segment motor neuron, with Scer\GAL4^nSyb.PS

Orp8^HMS01223

axon, with Scer\GAL4^nSyb.PS

Orp8^HMS01223

larval abdominal segment 7, with Scer\GAL4^nSyb.PS

Orp8^HMS01223

lipid droplet | increased number | third instar larval stage, with Scer\GAL4^nSyb.PS

Orp8^HMS01223

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\OSBPL5

12 of 15

Yes

Hsap\OSBPL8

12 of 15

Yes

Hsap\OSBPL9

3 of 15

Hsap\OSBPL11

3 of 15

Yes

Hsap\OSBPL10

2 of 15

Yes

Hsap\PAGE4

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Osbpl5

12 of 15

Yes

Mmus\Osbpl8

12 of 15

Yes

Mmus\Osbpl10

3 of 15

Yes

Mmus\Osbpl11

3 of 15

Yes

Mmus\Osbpl9

2 of 15

Mmus\Rps27rt

1 of 15

Rattus norvegicus (Norway rat) (5)

Rnor\Osbpl5

10 of 13

Yes

Rnor\Osbpl8

7 of 13

Yes

Rnor\Osbpl9

2 of 13

Rnor\Osbpl10

2 of 13

Yes

Rnor\Osbpl11

2 of 13

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\osbpl8

8 of 12

Yes

Xtro\osbpl9

3 of 12

Xtro\osbpl10

2 of 12

Yes

Xtro\osbpl11

2 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\osbpl5

10 of 15

Yes

Drer\osbpl8

5 of 15

Yes

Drer\LOC100334085

3 of 15

Yes

Drer\osbpl9

3 of 15

Drer\osbpl10b

3 of 15

Yes

Drer\osbpl11

2 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\obr-3

12 of 15

Yes

Cele\obr-4

2 of 15

Arabidopsis thaliana (thale-cress) (4)

Atha\ORP4A

3 of 9

Yes

Atha\ORP4B

2 of 9

Atha\ORP4C

2 of 9

Atha\AT1G77730

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\OSH7

8 of 15

Yes

Scer\OSH6

6 of 15

Yes

Scer\KES1

4 of 15

Scer\HES1

3 of 15

Yes

Scer\OSH3

1 of 15

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\SPCC23B6.01c

7 of 12

Yes

Spom\kes1

2 of 12

Spom\SPBC354.07c

1 of 12

Spom\SPBC646.08c

1 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

5 of 10

2 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

OSBPL5; oxysterol binding protein like 5

12 of 15

606733

OSBPL8; oxysterol binding protein like 8

12 of 15

606736

OSBPL9; oxysterol binding protein like 9

3 of 15

606737

OSBPL11; oxysterol binding protein like 11

3 of 15

606739

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Orp8 - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Orp8 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Orp8

enhanceable

Arl6IP1

(Byrne et al., 2022)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Arl6IP1

enhanceable

Orp8

(Byrne et al., 2022)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261550

MIST (protein-protein) - An integrated Molecular Interaction Database

CG42668

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Acyl chain remodelling of PS

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-68

Cytogenetic map

92E2-92E2

Sequence location

3R:20,309,344..20,324,196 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

92E2-92E2

Limits computationally determined from genome sequence between P{PZ}l(3)10585¹⁰⁵⁸⁵ and P{EP}Synd^EP409

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-68

(FlyBase, 2016)

3-72.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24702

w¹¹¹⁸; Mi{ET1}Orp8^MB04427

58300

y¹ v¹; P{TRiP.HMJ22407}attP40

34315

y¹ w^*; Mi{MIC}Orp8^MI02333

42441

y¹ w^*; Mi{MIC}Orp8^MI06855

82049

y¹ sc^* v¹ sev²¹; P{TKO.GS04490}attP40

34743

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01223}attP2

83870

y¹ sc^* v¹ sev²¹; P{SAM.dCas9.GS05309}attP40/CyO

67440

y¹ w^*; Mi{Trojan-GAL4.0}Orp8^{MI02333-TG4.0}/TM3, Sb¹ Ser¹

VDRC

v104813

P{KK111315}VIE-260B

v110074

P{KK108193}VIE-260B

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (44)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI12001

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261550

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261550

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

42412

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Orp8 CG42668

(Byrne et al., 2022)

Source for database merge of

Source for merge of: CG15684

(FlyBase Genome Annotators, 2010)

Additional comments

Annotations CG15684 and CG5077 merged as CG42668 in release 5.25 of the genome annotation.

(FlyBase Genome Annotators, 2010)

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 76 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42412

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4JCZ0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4JCZ0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261550

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261550

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42412/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261550

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261550

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5077

FlyMine - An integrated database for Drosophila genomics

FBgn0261550

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

42412

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC016041

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG42668

MARRVEL_MODEL - MARRVEL (model organism gene)

42412

modMine - A data warehouse for the modENCODE project

FBgn0261550

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261550

DRSC - Results frm RNAi screens

FBgn0261550

MIST (protein-protein) - An integrated Molecular Interaction Database

CG42668

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Acyl chain remodelling of PS

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Name Synonyms

Oxysterol-binding protein-related protein 8

oxysterol-binding protein-related protein 8

(Byrne et al., 2022)

Secondary FlyBase IDs

FBgn0038785
FBgn0038786

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (50)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections