FlyBase Gene Report: Dmel\CG42673

General Information

Symbol

Dmel\CG42673

Species

D. melanogaster

Name

Annotation Symbol

CG42673

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261555

Gene Model Status

Current

Stock Availability

34 publicly available

Gene Summary

Putative adapter protein. (UniProt, Q8SXX4)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

Dm_3L:44995

Key Links

Genomic Location

Cytogenetic map

67B13-67C1

Sequence location

3L:9,547,220..9,596,713 [+]

Recombination map

3-31

RefSeq locus

NT_037436 REGION:9547220..9596713

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables nitric-oxide synthase binding

inferred from biological aspect of ancestor with PANTHER:PTN000132727

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in postsynaptic actin cytoskeleton organization

inferred from sequence or structural similarity with UniProtKB:O75052

(Gene Ontology Curators, 2002-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in glutamatergic synapse

inferred from sequence or structural similarity with UniProtKB:O75052

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

Putative adapter protein.

(UniProt, Q8SXX4)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG42673 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8SXX4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG42673-RB

FBtr0302709

NM_168352

3618

698

CG42673-RC

FBtr0302710

NM_168353

3902

904

CG42673-RD

FBtr0302711

NM_001202149

3582

438

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG42673-PB

FBpp0291849

77.3

698

9.14

FBpp0291850

99.1

904

10.01

NP_729510

AAF50244

CG42673-PD

FBpp0291851

49.9

438

8.68

M9MRS2

NP_001189078

ADV37515

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG42673 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.81

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

anal pad primordium

Comment: reported as anal pad specific anlage

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG42673 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG42673

View exonic expression by tissue for Dmel\CG42673

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261555

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39111/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261555

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052048

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261555

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32048

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG42673

Transgenic constructs containing regulatory region of CG42673

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel6114

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

CG42673^GD8879

viable

CG42673⁰⁷

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

CG42673^GD8879

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (27)

Hsap\NOS1AP

11 of 14

Yes

Hsap\C1orf226

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\APBB2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC112267931

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (22)

Rnor\LOC100361087

11 of 14

Yes

Rnor\Nos1ap

10 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (23)

Mmus\Nos1ap

12 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (18)

Xtro\LOC100488020

5 of 13

Yes

Xtro\malt1

5 of 13

Yes

Xtro\nos1ap

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\entpd2

1 of 13

Xtro\f2r

1 of 13

Yes

Xtro\kif1a

1 of 13

Xtro\ldlrap1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (31)

Drer\nos1apa

11 of 14

Yes

Drer\si:dkey-34e4.1

11 of 14

Yes

Drer\nos1apb

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\ldlrap1b

2 of 14

Yes

Drer\mapk8ip2

2 of 14

Drer\numbl

2 of 14

Drer\si:dkey-71h2.2

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-204c21.1

1 of 14

Drer\si:dkey-19b23.8

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\dyc-1

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\jip-1

1 of 14

Cele\lin-10

1 of 14

Cele\num-1

1 of 14

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP007627

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG42673. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (11)

Aplip1

3 of 13

ced-6

3 of 13

CG8312

3 of 13

Dab

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

Shc

1 of 13

X11Lβ

1 of 13

X11L

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

NOS1AP; nitric oxide synthase 1 adaptor protein

model of nephrotic syndrome type 22

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NOS1AP; nitric oxide synthase 1 adaptor protein

11 of 14

605551

NEPHROTIC SYNDROME, TYPE 22; NPHS22

nephrotic syndrome type 22

C1orf226; chromosome 1 open reading frame 226

4 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261555

MIST (protein-protein) - An integrated Molecular Interaction Database

39111

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8SXX4

Class of Gene

nuclear_gene

protein_coding_gene

(FlyBase Genome Annotators, 2007)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-31

Cytogenetic map

67B13-67C1

Sequence location

3L:9,547,220..9,596,713 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

67B13-67C1

Limits computationally determined from genome sequence between P{EP}Hsp26^EP3336&P{EP}Hsp26^EP3315 and P{PZ}fry⁰²²⁴⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-31

(FlyBase, 2016)

3-23.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (34)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

72042

w¹¹¹⁸; P{VT028151-p65.AD}attP40

74050

w¹¹¹⁸; P{VT028145-p65.AD}attP40

Korea

12141

P{VT028145-GAL4}attP2

12421

P{VT028144-GAL4}attP2

13749

P{VT028151-GAL4}attP2

13750

P{VT028160-GAL4}attP2

17102

P{VT028145-lexA::GADfl}attP40

17103

P{VT028153-lexA::GADfl}attP40

17135

P{VT028144-lexA::GADfl}attP40

VDRC

v108571

P{KK101290}VIE-260B

v202424

P{VT028153-GAL4}attP2

v50237

w¹¹¹⁸ P{GD16604}v50237

More stocks available...

Genomic Clones (42)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (24)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261555

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE44718

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261555

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG34272) in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG32048 CG34272

(FlyBase Genome Annotators, 2010)

Source for merge of: CG32048 BcDNA:RE71585

(FlyBase, 1996-)

Source for merge of: CG14178 CG17357 CG3179

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: CG32048 BcDNA:RE71517

(FlyBase, 1996-)

Additional comments

Annotations CG32048 and CG34272 merged as CG42673 in release 5.25 of the genome annotation.

(FlyBase Genome Annotators, 2010)

Annotations CG14178, CG17357 and CG3179 merged as CG32048 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG32048 BcDNA:RE71585 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Source for merge of CG32048 BcDNA:RE71517 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

BcDNA:RE71517

BcDNA:RE71585

CG14178

CG17357

(Robertson, 2001.5.6)

CG3179

(Robertson, 2001.5.6)

CG32048

(Ashwal-Fluss et al., 2014, Hazelett et al., 2009, Keleman et al., 2009.8.5, Zhang et al., 2007)

CG34272

(FlyBase Genome Annotators, 2007)

CG42673

(Guzman et al., 2021, Meltzer et al., 2019, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, Ni et al., 2011.7.19, FlyBase Genome Annotators, 2010)

Dm_3L:44995

(FlyBase Genome Annotators, 2014, Ladoukakis et al., 2011)

Name Synonyms

Secondary FlyBase IDs

FBgn0052048
FBgn0036011
FBgn0036012
FBgn0040825
FBgn0061389
FBgn0064857
FBgn0085301

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39111

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8SXX4

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8SXX4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9MRS2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8SXX4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261555

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39111/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0261555

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0261555

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42673

MARRVEL_MODEL - MARRVEL (model organism gene)

39111

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261555

DroID - A comprehensive database of gene and protein interactions.

FBgn0261555

DRSC - Results frm RNAi screens

FBgn0261555

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052048

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0261555

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32048

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

39111

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8SXX4

References (44)

The following external sites may use different assemblies or annotations than FlyBase.

-0.81

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