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Gene Dmel\CG42674

General Information
Symbol	Dmel\CG42674	Species	D. melanogaster
Name		Annotation symbol	CG42674
Feature type	protein_coding_gene	FlyBase ID	FBgn0261556
Gene Model Status	Current	Stock availability	21 publicly available
Also Known As	CG7323, CG32223
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	77A1-77A1	Sequence location	3L:20,142,714..20,191,417 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG42674 (FBgn0261556). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: Rho guanyl-nucleotide exchange factor activity; phospholipid binding. There is experimental evidence that it is involved in the biological process: regulation of cell shape; cell adhesion; imaginal disc-derived leg morphogenesis. 25 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has 7 annotated transcripts and 7 annotated polypeptides. Protein features are: Dbl homology (DH) domain; Pleckstrin homology domain; Rho GTP exchange factor. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system, larval/adult midgut, larval Malpighian tubules, adult salivary gland. Gene sequence location is 3L:20142714..20191417.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 77A1-77A1   Limits computationally determined from genome sequence between P{lacW}l(3)L1243L1243&P{lacW}Mi-2j3D4 and P{PZ}eRF100103
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG42674 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene merge based on EST/cDNA data. Gene model reviewed during 5.45 Gene merge based on RNAseq data. Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG42674-RA FBtr0302712  NM_001144512   10165   1626 CG42674-RB FBtr0302713  NM_001144513   9727   1480 CG42674-RC FBtr0302714  NM_140925   10270   1661 CG42674-RD FBtr0302715  NM_001144514   9513   1445 CG42674-RE FBtr0302716  NM_001202208   7058   1412 CG42674-RF FBtr0332698  NM_001275161   6949   1412 CG42674-RG FBtr0332699  NM_001275162   5003   848
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG42674-PA   FBpp0291852   173.7   1626   8.04     NP_001137984   ACL83339 CG42674-PB   FBpp0291853   159.2   1480   7.75     NP_001137985   ACL83340 CG42674-PC   FBpp0291854   177.4   1661   8.15     NP_649182   AAF49064 CG42674-PD   FBpp0291855   155.5   1445   7.59     NP_001137986   ACL83341 CG42674-PE   FBpp0291856   156.3   1412   9.67     NP_001189137   ADV37573 CG42674-PF   FBpp0304944   156.3   1412   9.67     NP_001262090   AGB94783 CG42674-PG   FBpp0304945   93.3   848   7.79     NP_001262091   AGB94784
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dbl homology (DH) domain (IPR000219) Pleckstrin homology domain (IPR001849) Rho GTP exchange factor (IPR015721)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF49064   AE014296 ACL83339   AE014296 ACL83340   AE014296 ACL83341   AE014296 ADV37573   AE014296 AGB94783   AE014296 AGB94784   AI296208   AI533109   AW944387   AY061597 AAL29145   AY094943 AAM11296   BI164651   BI167086   BI586695   BI628512   CL528217   CO196185   CO276774   CO277446   CO284931   CO298094   CO309097   CO310522   CO342097   CO343180   CZ466836   CZ469748   CZ470083   CZ470128   CZ471132   CZ471167   CZ478097   CZ479804   CZ483119   CZ490799   EC056724   EC064733   EC077777   EC083064   EC220626   EC221548   EC236619   EC252230   EC259545   EL883260   EL883491   EV600672   GH778397   GH787279   GR705717   GR705733   GR705798
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	B7Z086 B7Z087 B7Z088 Q9VW85 Q8SWY9 Q95R62
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system, larval/adult midgut, larval Malpighian tubules, adult salivary gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    6 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    6 head, mated 20-day female    5 head, mated 1-day male    12 head, mated 4-day male    10 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    8 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    2 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    6 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    6 head, mated 20-day female    5 head, mated 1-day male    12 head, mated 4-day male    10 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    8 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    2 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    6 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    6 head, mated 20-day female    5 head, mated 1-day male    12 head, mated 4-day male    10 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    8 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    2 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    6 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    6 head, mated 20-day female    5 head, mated 1-day male    12 head, mated 4-day male    10 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    8 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    2 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    6 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    6 head, mated 20-day female    5 head, mated 1-day male    12 head, mated 4-day male    10 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    8 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    2 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    6 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    6 head, mated 20-day female    5 head, mated 1-day male    12 head, mated 4-day male    10 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    8 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    2 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    6 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    6 head, mated 20-day female    5 head, mated 1-day male    12 head, mated 4-day male    10 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    8 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    2 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    6 central nervous system, pupae P8    11 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    5 head, mated 1-day female    7 head, mated 4-day female    6 head, mated 20-day female    5 head, mated 1-day male    12 head, mated 4-day male    10 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    8 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    2 carcass, larvae L3 wandering    4 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    5 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    8 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    14 larva L1    7 larva L2    4 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    6 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    5 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    7 adult female 05day    7 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    5 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    8 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    14 larva L1    7 larva L2    4 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    6 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    5 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    7 adult female 05day    7 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    5 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    8 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    14 larva L1    7 larva L2    4 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    6 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    5 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    7 adult female 05day    7 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    5 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    8 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    14 larva L1    7 larva L2    4 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    6 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    5 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    7 adult female 05day    7 adult female 30day    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    5 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    8 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    14 larva L1    7 larva L2    4 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    6 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    5 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    7 adult female 05day    7 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    5 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    8 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    14 larva L1    7 larva L2    4 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    6 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    5 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    7 adult female 05day    7 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    5 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    8 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    14 larva L1    7 larva L2    4 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    6 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    5 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    7 adult female 05day    7 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    5 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    4 embryo 12-14hr    4 embryo 14-16hr    8 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    14 larva L1    7 larva L2    4 larva L3 12hr old    4 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    6 white prepupae new    6 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    4 pupae 3d postWPP    2 pupae 4d postWPP    5 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    7 adult female 05day    7 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    2 embryonic GM2    17 embryonic Kc167    6 embryonic S1    18 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    40 wing disc ML-DmD8    8 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    8 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    6 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    12 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    2 embryonic GM2    17 embryonic Kc167    6 embryonic S1    18 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2  (40) wing disc ML-DmD8    8 wing disc ML-DmD9  (33) wing disc ML-DmD16-c3    11 wing disc ML-DmD21    8 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    6 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    12 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    2 embryonic GM2    17 embryonic Kc167    6 embryonic S1    18 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    40 wing disc ML-DmD8    8 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    8 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    6 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    12 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    2 embryonic GM2    17 embryonic Kc167    6 embryonic S1    18 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    40 wing disc ML-DmD8    8 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    8 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    6 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    12 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    2 embryonic GM2    17 embryonic Kc167    6 embryonic S1    18 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    40 wing disc ML-DmD8    8 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    8 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    6 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    12 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    2 embryonic GM2    17 embryonic Kc167    6 embryonic S1    18 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2  (40) wing disc ML-DmD8    8 wing disc ML-DmD9  (33) wing disc ML-DmD16-c3    11 wing disc ML-DmD21    8 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    6 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    12 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    2 embryonic GM2    17 embryonic Kc167    6 embryonic S1    18 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    40 wing disc ML-DmD8    8 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    8 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    6 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    12 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    2 embryonic GM2    17 embryonic Kc167    6 embryonic S1    18 embryonic S3    10 leg disc CME L1    12 wing disc CME-W2    40 wing disc ML-DmD8    8 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    8 wing disc ML-DmD32    15 haltere disc ML-DmD17-c3    6 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    2 tumorous blood cells mbn2    12 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    8 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    7 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • RH56938 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036946
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 CG42674GD5131 viable CG42674EY10278 CG42674f00650 viable, with Scer\GAL4pnr-MD237 CG42674GD16755 Sterility Allele fertile CG42674EY10278 CG42674f00650 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 CG42674GD16755 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 CG42674GD16755
Classical Alleles ( 17 )
For All Classical Alleles Show
Allele of CG42674 Class Mutagen Stocks Known lesion CG42674f00650 piggyBac transposase 2 -- CG42674c01042 piggyBac transposase 1 -- CG42674c04284 1 -- CG42674c05112 piggyBac transposase 1 -- CG42674c05544 piggyBac transposase 1 -- CG42674c05602 piggyBac transposase 1 -- CG42674e00216 piggyBac transposase 1 -- CG42674e01995 piggyBac transposase 1 -- CG42674EY10278 P-element activity 1 -- CG42674f08097 piggyBac transposase 1 -- CG42674KG00413 1 -- CG42674MI04021 1 -- CG42674MI04330 1 -- CG42674MI06792 1 -- CG42674c06971 piggyBac transposase 0 -- CG42674c07018 piggyBac transposase 0 -- CG42674DG23102 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG42674 Class Mutagen Stocks Known lesion CG42674GD16755 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG42674GD5131 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG42674HMS00228 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG42674JF01659 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG42674KK105072 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG42674GD8124 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes CG42674NIG.13811R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes CG42674NIG.7323R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3L)Ssk (Yanagihashi et al., 2012)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.7323R} NA P{NIG.13811R} NA P{TRiP.HMS00228} NA P{TRiP.JF01659} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}CG42674EY10278 NA P{GSV1}GSd217GSd217 NA miscellaneous insertions Mi{MIC}CG42674MI04021 NA Mi{MIC}CG42674MI04330 NA Mi{MIC}CG42674MI06792 NA P{SUPor-P}CG42674KG00413 NA P{wHy}CG42674DG23102 NA PBac{PB}CG42674c01042 NA PBac{PB}CG42674c04284 NA PBac{PB}CG42674c05112 NA PBac{PB}CG42674c05544 NA PBac{PB}CG42674c05602 NA PBac{PB}CG42674c06971 NA PBac{PB}CG42674c07018 NA PBac{RB}CG42674e00216 NA PBac{RB}CG42674e01995 NA PBac{WH}CG42674f00650 NA PBac{WH}CG42674f08097 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell adhesion inferred from mutant phenotype (Kiger et al., 2003) imaginal disc-derived leg morphogenesis inferred from mutant phenotype (Greenberg and Hatini, 2011) regulation of cell shape inferred from mutant phenotype (Kiger et al., 2003)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
phospholipid binding inferred from electronic annotation with InterPro:IPR001849 (FlyBase Curators et al., 2004-) Rho guanyl-nucleotide exchange factor activity inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
regulation of Rho protein signal transduction inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
CG42674 - CG31637 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG42674 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261556 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40205
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6XWGH8 Dipteran inclusive ortholog search EOG6DFPWM Insect inclusive ortholog search EOG6NK9QG Arthropod inclusive ortholog search EOG6J3V01 Metazoa inclusive ortholog search EOG60PH0V
Orthologs in Drosophila Species (EOG6XWGH8)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG42674     Drosophila simulans   Dsim\GD12217 Y   Drosophila sechellia   Dsec\GM22246 Y   Drosophila erecta   Dere\GG13340 Y   Drosophila yakuba   Dyak\GE22946 Y   Drosophila ananassae   Dana\GF24485     Drosophila pseudoobscura pseudoobscura   Dpse\GA23600 Y   Drosophila persimilis   Dper\GL25056     Drosophila willistoni   Dwil\GK17249 Y   Drosophila virilis   Dvir\GJ11546 Y   Drosophila mojavensis   Dmoj\GI11526 Y   Drosophila grimshawi   Dgri\GH16335 Y
Orthologs in non-Drosophila Dipterans (EOG6DFPWM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007721   Anopheles gambiae Malaria mosquito  Agam\AGAP010473   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ007917
Orthologs in non-Dipteran Insects (EOG6NK9QG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB54496   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG036935   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16120   Atta cephalotes Leafcutter ant  Acep\ACEP23414   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22789   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16198   Pogonomyrmex barbatus Red harvester ant  Pbar\PB23636   Solenopsis invicta Red fire ant  Sinv\SINV20908   Bombyx mori Silkmoth  Bmor\BGIBMGA012299   Pediculus humanus Human body louse  Phum\PHUM155340   Tribolium castaneum Red flour beetle  Tcas\TC014600
Orthologs in non-Insect Arthropods (EOG6J3V01)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW008875
Orthologs in non-Arthropod Metazoa (EOG60PH0V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\rhgf-2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014333gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_026073gn   Danio rerio Zebrafish  Drer\CABZ01057013.1   Danio rerio Zebrafish  Drer\plekhg5a   Danio rerio Zebrafish  Drer\plekhg6   Xenopus tropicalis Western clawed frog  Xtro\plekhg5   Mus musculus House mouse  Mmus\ENSMUSG00000039713   Mus musculus House mouse  Mmus\Plekhg6   Rattus norvegicus Norway rat  Rnor\Plekhg6   Rattus norvegicus Norway rat  Rnor\Plekhg5   Homo sapiens Human  Hsap\ENSG00000171680   Homo sapiens Human  Hsap\PLEKHG6
Human Orthologs (2)
Gene OMIM HGNC Hsap\ENSG00000171680     Hsap\PLEKHG6
AAA Orthologs (29) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12215 Drosophila simulans Dsim\GD12216 Drosophila simulans Dsim\GD12217 Drosophila sechellia Dsec\GM22244 Drosophila sechellia Dsec\GM22245 Drosophila sechellia Dsec\GM22246 Drosophila erecta Dere\GG13338 Drosophila erecta Dere\GG13339 Drosophila erecta Dere\GG13340 Drosophila yakuba Dyak\GE22944 Drosophila yakuba Dyak\GE22945 Drosophila yakuba Dyak\GE22946 Drosophila ananassae Dana\GF24484 Drosophila pseudoobscura pseudoobscura Dpse\GA16764 Drosophila pseudoobscura pseudoobscura Dpse\GA23599 Drosophila pseudoobscura pseudoobscura Dpse\GA23600 Drosophila persimilis Dper\GL25055 Drosophila willistoni Dwil\GK17247 Drosophila willistoni Dwil\GK17248 Drosophila willistoni Dwil\GK17249 Drosophila virilis Dvir\GJ11544 Drosophila virilis Dvir\GJ11545 Drosophila virilis Dvir\GJ11546 Drosophila mojavensis Dmoj\GI11524 Drosophila mojavensis Dmoj\GI11525 Drosophila mojavensis Dmoj\GI11526 Drosophila grimshawi Dgri\GH16332 Drosophila grimshawi Dgri\GH16333 Drosophila grimshawi Dgri\GH16335
Stocks & Reagents
Stocks Listed in FlyBase ( 21 )
Harvard	- PBac{PB}CG42674c01042 - PBac{PB}CG42674c04284 - PBac{PB}CG42674c05112 - PBac{PB}CG42674c05544 - PBac{PB}CG42674c05602 - PBac{RB}CG42674e00216 - PBac{RB}CG42674e01995 - PBac{WH}CG42674f00650 - PBac{WH}CG42674f08097
VDRC	v100153 P{KK105072}VIE-260B v15631 w1118; P{GD5131}v15631 v46770 w1118; P{GD16755}v46770
	More stocks available...
Genomic Clones ( 1 )
	BACR31C03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 30 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RH56938 SD04822
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP10046 RE04365 RE07382 RH26780
Other clones	523 5413 18292 99560 100087 157317 214247.54 214279.55 CK01.112C.H3 Dmel_neb_015033_0917_2849 EK001730 EK122026 EK124446 EK167610 EK171501 EK221925 EK260310 EP13827 EP16243 IP12955 IP13155 MB6-CG42348-RD RP003240514 RP003443289
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261556 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40205
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG13811 CG7323 (FlyBase Genome Annotators, 2008) Source for merge of: CG13812 CG32223 (FlyBase, 1996-) Source for merge of: CG32223 CG42348 (FlyBase Genome Annotators, 2010)
Additional comments	Annotations CG32223 and CG42348 merged as CG42674 in release 5.25 of the genome annotation. (FlyBase Genome Annotators, 2010) Annotations CG13811 and CG7323 merged as CG42348 in release 5.9 of the genome annotation. (FlyBase Genome Annotators, 2008) Source for merge of CG13812 CG32223 was a shared cDNA (date:030728). (FlyBase, 1996-)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become round and detached. Kc167 cells are unaffected. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Annotation CG13812 renamed CG32223 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140925 NM_001144512 NM_001144513 NM_001144514 NM_001202208 NM_001275161 NM_001275162 RefSeq (Proteins) • NP_649182 NP_001137984 NP_001137985 NP_001137986 NP_001189137 NP_001262090 NP_001262091 DDBJ / EMBL / GenBank • AAF49064 AAL29145 AAM11296 ACL83339 ACL83340 ACL83341 ADV37573 AGB94783 AGB94784 AI533109 AW944387 AY061597 AY094943 BI628512 CL528217 CZ466836 CZ469748 CZ470083 CZ470128 CZ471132 CZ471167 CZ478097 CZ479804 CZ483119 CZ490799 Entrez Gene - A searchable database of RefSeq genes. • 40205 326201 UniProtKB/TrEMBL • B7Z086 B7Z087 B7Z088 Q8SWY9 Q9VW85 Q95R62
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dbl homology (DH) domain (IPR000219) Pleckstrin homology domain (IPR001849) Rho GTP exchange factor (IPR015721) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RH56938
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261556 DRSC - Results from RNAi screens. • FBgn0261556 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0036946 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261556 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40205 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40205 modMine - Data generated by the modENCODE project. • FBgn0261556
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG7323 (Greenberg and Hatini, 2011, Bakal et al., 2007, Japanese National Institute of Genetics, 2012.5.21) CG13811 (Japanese National Institute of Genetics, 2012.5.21) CG13812 (Mount, 2001.8.14, ) CG32223 (Minidorff et al., 2007, Minidorff et al., 2007, Keleman et al., 2009.8.5) CG42348 (FlyBase Genome Annotators, 2008) CG42674 (FlyBase Genome Annotators, 2010, Ni et al., 2008, Ni et al., 2011.7.19, Yanagihashi et al., 2012)
Name Synonym	CG7323   CG13811
Secondary FlyBase IDs
	FBgn0036943 FBgn0036944 FBgn0036946 FBgn0052223 FBgn0259679
References ( 37 )
Generate a list of	All references relating to this gene ( 37 )
List References by type	Research paper  ( 5 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 15 ) FlyBase analysis  ( 6 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Yanagihashi et al., 2012, J. Cell Sci. 125(8): 1980--1990 Snakeskin, a membrane protein associated with smooth septate junctions, is required for intestinal barrier function in Drosophila. [FBrf0218431] Greenberg and Hatini, 2011, Mech. Dev. 128(1-2): 5--17 Systematic expression and loss-of-function analysis defines spatially restricted requirements for Drosophila RhoGEFs and RhoGAPs in leg morphogenesis. [FBrf0212976] Show all research papers ...