l(2)br1, BG:DS03792.2 , l(2)34Fb, laminin α1,2, BG:DS03792.1
Gene model reviewed during 5.42
Gene model reviewed during 5.51
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
11 (northern blot)
3367 (aa); 360 (kD observed); 374 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\wb using the Feature Mapper tool.
11kb wb trancripts are first detected in early stages of embryogenesis and peak in 6-12hr embryos on northern blots. Transcripts are barely detectable in third instar larvae and increase again in pupal stages. A weak 10.5kb is also detected in later embryos. wb transcripts are first detected by in situ hybridization during oogenesis in nurse cells and growing oocytes. Transcripts are uniformly distributed in cleavage stage embryos and are enriched in cells of the trunk region in blastoderm embryos. During germ band extension, wb tr nscripts are uniformly distributed. After germ band retraction, transcripts accumulate in the visceral mesoderm, near muscle attachment sites, and in cardiac cells.
wb protein is detected in the cytoplasm of wing disc cells and at the interface of their contact with tracheal cells starting at embryonic stage 14. It is not detected in tracheal cells or in the surrounding epidermis.
In situ hybridization shows that wb is enriched in border follicle cells relative to follicle cells in stage S9 or S10 egg chambers.
wb protein is first observed in stage 10 embryos as a weak diffusive stripe between the ectoderm and the mesoderm. During germ band retraction, the protein is localized diffusely in the visceral mesoderm. At stage 14, strong staining is observed in the basement membranes that surround the digestive system and at muscle attachment sites. In later stages, staining is observed in dorsal structures along the ventral nerve cord and in basement membranes around the digestive system. wb protein is also observed in developing wing imaginal d scs in a spot pattern on the presumptive wing dorsal and ventral region.
GBrowse - Visual display of RNA-Seq signalsView Dmel\wb in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
New annotation (CG32970) in release 3 of the genome annotation.
wb has been characterised as a potential ligand of PS2 integrins by functional interaction in cell culture.
Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis.
Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.
One allele in this group has bent down wings when crossed to either the other lethals in the group or with deletions that included this locus.
Most of the mutants are characterized by homo- or hemizygous lethality, but a visible phenotype has also been reported. O'Donnell et al., 1977 describe a bent-down-wing phenotype occurring when one of their lethals is crossed to other lethals in the same complementation group and the same phenotype when three of their lethals are crossed to deletions for the locus. Woodruff and Ashburner, 1979 describe the viable and fertile wing blister phenotype (a roughly circular area around the crossveins of the wing, forming a blister which often collapses soon after eclosion, distorting the wing blade). The allele showing this phenotype (wb1) is viable and fertile in homozygotes and hemizygotes as well as in heteroallelic combinations with lethal alleles.