FlyBase Gene Report: Dmel\wb

General Information

Symbol

Dmel\wb

Species

D. melanogaster

Name

wing blister

Annotation Symbol

CG42677

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261563

Gene Model Status

Current

Stock Availability

34 publicly available

Gene Snapshot

wing blister (wb) encodes one of 2 laminin α chains which assembles into a Laminin molecule. Laminin is an extracellular matrix component that interacts with integrins and mediates cell adhesion, guidance, signalling and basement membrane integrity. [Date last reviewed: 2019-03-21]

Other Summaries

Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

l(2)br1, BG:DS03792 .2, l(2)34Fb, A1, laminin α1,2

Key Links

Genomic Location

Cytogenetic map

35A3-35A4

Sequence location

2L:14,263,027..14,328,262 [+]

Recombination map

2-50

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

LAMININS

Protein Family (UniProt)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

Predictions / Assertions

Summaries

Gene Group (FlyBase)

LAMININS -: Laminins are major structural components of the basement membrane. (Adapted from FBrf0230242.)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

wb: wing blister

Most of the mutants are characterized by homo- or hemizygous lethality, but a visible phenotype has also been reported. O'Donnell et al., 1977, describe a bent-down-wing phenotype occurring when one of their lethals is crossed to other lethals in the same complementation group and the same phenotype when three of their lethals are crossed to deletions for the locus. Woodruff and Ashburner, 1979, describe the viable and fertile wing blister phenotype (a roughly circular area around the crossveins of the wing, forming a blister which often collapses soon after eclosion, distorting the wing blade). The allele showing this phenotype (wb1) is viable and fertile in homozygotes and hemizygotes as well as in heteroallelic combinations with lethal alleles.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\wb or the JBrowse view of Dmel\wb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.51

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

wb-RB

FBtr0302731

NM_165086

11333

3375

wb-RC

FBtr0302732

NM_001201877

8975

2567

wb-RD

FBtr0302733

NM_001201878

9057

2567

wb-RE

FBtr0302734

NM_001201879

9183

2567

wb-RF

FBtr0302735

NM_001201880

8996

2567

wb-RG

FBtr0305063

NM_057442

10270

3001

Additional Transcript Data and Comments

Reported size (kB)

11 (northern blot)

(Martin et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

wb-PB

FBpp0291871

374.7

3375

6.79

NP_723870

AAN10875

wb-PC

FBpp0291872

287.7

2567

8.40

NP_001188806

ADV37056

wb-PD

FBpp0291873

287.7

2567

8.40

NP_001188807

ADV37057

wb-PE

FBpp0291874

287.7

2567

8.40

NP_001188808

ADV37058

wb-PF

FBpp0291875

287.7

2567

8.40

NP_001188809

ADV37059

wb-PG

FBpp0293600

332.7

3001

6.61

NP_476790

AAF53381

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2567 aa isoforms: wb-PC, wb-PD, wb-PE, wb-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

3367 (aa); 360 (kD observed); 374 (kD predicted)

(Martin et al., 1999)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\wb using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

wb_1
wb_2

Linkouts

Gene Ontology (7 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

digestive tract mesoderm development

inferred from mutant phenotype

(Weyers et al., 2011)

epithelial structure maintenance

inferred from mutant phenotype

(Martin et al., 1999)

gonad development

inferred from mutant phenotype

(Weyers et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

animal organ morphogenesis

inferred from biological aspect of ancestor with PANTHER:PTN002576803

(assigned by GO_Central )

(Gaudet et al., 2011)

axon guidance

inferred from biological aspect of ancestor with PANTHER:PTN000708002

(assigned by GO_Central )

(Gaudet et al., 2011)

tissue development

inferred from biological aspect of ancestor with PANTHER:PTN002576803

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

basement membrane

inferred from direct assay

(Martin et al., 1999)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

basement membrane

inferred from biological aspect of ancestor with PANTHER:PTN000832138

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 4

organism | ubiquitous | uniform

(Martin et al., 1999)

embryonic stage 13 -- 17

visceral mesoderm

(Martin et al., 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Martin et al., 1999)

embryonic stage 2

organism | ubiquitous

(Martin et al., 1999)

embryonic stage 9 -- 11

organism | ubiquitous

(Martin et al., 1999)

embryonic stage 13 -- 17

muscle attachment site | vicinity of

(Martin et al., 1999)

embryonic stage 14

alary cell

(Martin et al., 1999)

adult stage & oogenesis

oocyte

(Martin et al., 1999)

nurse cell

(Martin et al., 1999)

Additional Descriptive Data

11kb wb trancripts are first detected in early stages of embryogenesis and peak in 6-12hr embryos on northern blots. Transcripts are barely detectable in third instar larvae and increase again in pupal stages. A weak 10.5kb is also detected in later embryos. wb transcripts are first detected by in situ hybridization during oogenesis in nurse cells and growing oocytes. Transcripts are uniformly distributed in cleavage stage embryos and are enriched in cells of the trunk region in blastoderm embryos. During germ band extension, wb tr nscripts are uniformly distributed. After germ band retraction, transcripts accumulate in the visceral mesoderm, near muscle attachment sites, and in cardiac cells.

(Martin et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

mesectoderm

(Martin et al., 1999)

embryonic stage 12 -- 17

visceral mesoderm

(Martin et al., 1999)

embryonic stage 14

muscle attachment site

(Martin et al., 1999)

embryonic stage 15 -- 17

ventral nerve cord | dorsal

(Martin et al., 1999)

third instar larval stage

wing disc | restricted

(Martin et al., 1999)

wing pouch | restricted

Comment: in a spot pattern

(Martin et al., 1999)

Additional Descriptive Data

wb protein is first observed in stage 10 embryos as a weak diffusive stripe between the ectoderm and the mesoderm. During germ band retraction, the protein is localized diffusely in the visceral mesoderm. At stage 14, strong staining is observed in the basement membranes that surround the digestive system and at muscle attachment sites. In later stages, staining is observed in dorsal structures along the ventral nerve cord and in basement membranes around the digestive system. wb protein is also observed in developing wing imaginal d scs in a spot pattern on the presumptive wing dorsal and ventral region.

(Martin et al., 1999)

Marker for

Subcellular Localization

CV Term

Evidence

References

basement membrane

inferred from direct assay

(Martin et al., 1999)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\wb in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\wb

View exonic expression by tissue for Dmel\wb

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0004002

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CK02229

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 84 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of wb

Transgenic constructs containing regulatory region of wb

Deletions and Duplications ( 124 )

Not disrupted in

Df(2L)A48

(Fossett et al., 1994, Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A63

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A72

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A178

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A217

(Roote, 2009.11.24, Roote, 2000.7.3, Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A220

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A245

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A260

(Ashburner et al., 1982)

Df(2L)A264

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A266

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A267

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A379

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A400

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A445

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)Adh-nBR102

(Fossett et al., 1994)

Df(2L)Adh-nBR105

(Fossett et al., 1994)

Df(2L)Adh-nBR106

(Fossett et al., 1994)

Df(2L)Adh-nBR107

(Fossett et al., 1994)

Df(2L)Adh-nBR118

(Fossett et al., 1994)

Df(2L)Adh-nBR119

(Fossett et al., 1994)

Df(2L)Adh-nBR121

(Fossett et al., 1994)

Df(2L)Adh-nBR122

(Fossett et al., 1994)

Df(2L)Adh-nBR126

(Fossett et al., 1994)

Df(2L)Adh-nBR127

(Fossett et al., 1994)

Df(2L)Adh-nBR128

(Fossett et al., 1994)

Df(2L)Adh-nBR129

(Fossett et al., 1994)

Df(2L)b75

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)b77c

(Roote, 2000.7.3)

Df(2L)b80c1

(Ashburner et al., 1982)

(Roote, 2000.7.3)

(Roote, 1996.8)

(Roote, 1996.8)

(Roote, 2000.7.3)

(Roote, 2000.7.3)

(Roote, 2000.6.13)

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn3

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn15

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn27

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)Sco^rv4

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)TE35B-1

(Gubb et al., 1985)

Df(2L)TE35B-6

(Gubb et al., 1985)

Df(2L)TE35B-7

(Gubb et al., 1985)

Df(2L)TE35B-11

(Gubb et al., 1985)

Df(2L)TE35B-12

(Gubb et al., 1985)

Df(2L)TE35BC-1

(Gubb et al., 1984)

Df(2L)TE35BC-3

(Gubb et al., 1984)

Df(2L)TE35BC-4

(Gubb et al., 1984)

Df(2L)TE35BC-7

(Gubb et al., 1984)

Df(2L)TE35BC-8

(Gubb et al., 1984)

Df(2L)TE35BC-24

(Gubb et al., 1984)

Df(2L)TE35BC-28

(Gubb et al., 1984)

Df(2L)TE35BC-34

(Gubb et al., 1984)

Df(2L)TE35BC-35

(Gubb et al., 1984)

In(2L)TE35B-13

(Gubb et al., 1985)

T(2;3)H147

(Roote, 1997.6.2)

T(2;3)Sco^rv7

(Woodruff and Ashburner, 1979)

T(Y;2)A80^DR15^P

(Woodruff and Ashburner, 1979)

T(Y;2)J165^DP58^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(Y;2)R15^DJ165^P

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Tp(2;2)A446

(Fossett et al., 1994, Ashburner et al., 1982)

Disrupted in

Df(2L)64j

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)A47

(Fossett et al., 1994, Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A215

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A246

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A263

(Ashburner et al., 1982)

Df(2L)A337

(Fossett et al., 1994)

Df(2L)A376

(Fossett et al., 1994, Ashburner et al., 1982)

Df(2L)A377

(Ashburner et al., 1982)

Df(2L)Adh-nBR100

(Roote, 1996.1.11, Roote, 1996.8, Roote et al., 1996, Fossett et al., 1994)

Df(2L)Adh-nBR101

(Fossett et al., 1994)

Df(2L)Adh-nBR103

(Roote, 1996.1.11, Roote, 1996.8, Fossett et al., 1994)

Df(2L)Adh-nBR117

(Fossett et al., 1994)

Df(2L)Adh-nBR120

(Roote, 1996.1.11, Roote, 1996.8, Fossett et al., 1994)

Df(2L)Adh-nBR125

(Roote, 1996.1.11, Roote, 1996.8, Fossett et al., 1994)

Df(2L)Adh-nBR130

(Fossett et al., 1994)

Df(2L)Adh-nBR131

(Fossett et al., 1994)

Df(2L)b75

(Roote, 2000.7.3, Gubb et al., 1990)

Df(2L)b78j

(Roote, 2009.11.24, Roote, 2000.7.3, Alexandrova et al., 1996, Roote, 1995.3.27)

Df(2L)b79b4

(Roote, 2000.7.3, Alexandrova et al., 1996, Roote, 1995.3.27)

Df(2L)b84a7

(BDGP Project Members, 1994-1999)

Df(2L)b84h50

(BDGP Project Members, 1994-1999)

Df(2L)b85f1

(Roote, 2000.7.3, Roote, 1995.3.27)

Df(2L)ED8185

(Roote, 2009.11.24, Roote, 2009.11.24)

Df(2L)ED8186

(Roote, 2009.11.24, Roote, 2009.11.24)

Df(2L)el17

(Roote et al., 1996)

Df(2L)el77

(Roote et al., 1996, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

Df(2L)el80f1

(Schock and Perrimon, 2003)

Df(2L)el81i1

(Roote et al., 1996)

Df(2L)fn1

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn5

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn7

(Martin et al., 1999, Gubb et al., 1985, Ashburner et al., 1982, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn12

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn26

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn30

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn31

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn36

(Martin et al., 1999, Roote et al., 1996, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)GT4

(Gubb et al., 1990)

Df(2L)GT5

(Gubb et al., 1990)

Df(2L)k07245-mr11

(Roote, 2000.6.13)

Df(2L)k11509-rv18

(Roote, 2000.6.13)

Df(2L)noc13

(Roote et al., 1996)

Df(2L)osp38

(Roote, 1996.8)

Df(2L)rN149-rv3

(Roote, 2000.6.13)

Df(2L)TE35B-2

(Gubb et al., 1985)

Df(2L)TE35B-3

(Gubb et al., 1985)

Df(2L)TE35B-4

(Gubb et al., 1985)

Df(2L)TE35B-5

(Gubb et al., 1985)

Df(2L)TE35B-8

(Gubb et al., 1985)

Df(2L)TE35B-9

(Gubb et al., 1985)

Df(2L)TE35B-10

(Roote et al., 1996, Gubb et al., 1985)

Df(2L)TE35B-15

(Roote et al., 1996, Gubb et al., 1985)

Df(2L)TE35B-300

(Roote et al., 1996)

Df(2L)TE35BC-6

(Gubb et al., 1984)

Df(2L)TE35BC-23

(Gubb et al., 1984)

Df(2L)TE35BC-29

(Gubb et al., 1984)

Df(2L)TE35BC-31

(Gubb et al., 1984)

Df(2L)TE35D-24

(Roote, 1998.3.17)

Df(2L)W

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)75c^LC158.1^R

(Roote et al., 1996, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

In(2L)75c^LC163^R

In(2L)75c

(Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

T(2;4)GT6

(Roote, 1998.6.3, Gubb et al., 1990)

Ts(2Lt;3Lt)H147+Ts(2Rt;3Rt)TE35B-8

(Roote et al., 1996)

Ts(YSt;2Lt)G74+Ts(YSt;2Rt)A80

(Woodruff and Ashburner, 1979)

Partially disrupted in

T(2;4)GT6

(Roote, 1999.6.8)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^Act5C.PU

wb^GD1560

lethal (with Df(2L)ED8186)

wb^{MI07688-TG4.1}

lethal | recessive

lethal - all die before end of embryonic stage | recessive

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

wb^GD1560

viable, with Scer\GAL4^Mef2.PR

wb^GD1560

viable, with Scer\GAL4^pnr-MD237

wb^GD1560

viable, with Scer\GAL4^tin.CΔ4

wb^GD1560

Sterility

Allele

fertile

Other Phenotypes

Allele

neuroanatomy defective | third instar larval stage, with Scer\GAL4^hh-Gal4

wb^GD1560

size defective | adult stage, with Scer\GAL4^nub.PU

wb^GD1560

some die during embryonic stage | recessive

visible, with Scer\GAL4^pnr-MD237

wb^GD1560

visible | recessive

Phenotype manifest in

Allele

chaeta | supernumerary, with Scer\GAL4^pnr-MD237

wb^GD1560

embryo | gastrula stage

embryo | germline clone

wb^4Y18

embryonic/first instar larval cuticle | dorsal

embryonic/larval dorsal trunk

embryonic/larval dorsal vessel

embryonic/larval pericardial cell

embryonic/larval salivary gland

wb^4Y18

embryonic/larval somatic muscle

embryonic midgut chamber

wb^SF25

extracellular matrix

wb^SF25

eye photoreceptor cell | somatic clone

wb^k05612

germ band | germline clone

wb^4Y18

gonad | embryonic stage (with wb^37.01)

wb^146.21

gonad | embryonic stage (with wb^146.21)

wb^37.01

gonadal sheath proper primordium | embryonic stage (with wb^37.01)

wb^146.21

gonadal sheath proper primordium | embryonic stage (with wb^146.21)

wb^37.01

larval dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^hh-Gal4

wb^GD1560

ventral oblique muscle

visceral mesoderm | embryonic stage (with wb^37.01)

wb^146.21

visceral mesoderm | embryonic stage (with wb^146.21)

wb^37.01

wing

wing, with Scer\GAL4^Bx-MS1096

wb^GD1560

wing, with Scer\GAL4^nub.PU

wb^GD1560

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\LAMA1

10 of 15

Yes

Hsap\LAMA2

9 of 15

Yes

3 of 15

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Lama1

10 of 15

Yes

Mmus\Lama2

8 of 15

Yes

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (10)

Rnor\Lama1

8 of 13

Yes

Rnor\Lama2

3 of 13

Yes

Rnor\Lamc1

2 of 13

Rnor\Agrn

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (6)

Xtro\lama1

4 of 12

Yes

Xtro\lama3

2 of 12

Xtro\lama2

1 of 12

Yes

Xtro\lama4

1 of 12

Xtro\lama5

1 of 12

Xtro\lamc1

1 of 12

Danio rerio (Zebrafish) (12)

Drer\lama1

6 of 15

Yes

Drer\lama2

2 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\lam-3

8 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\IMH1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919001W )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\wb

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13629

Drosophila persimilis

Dper\GL21262

Drosophila willistoni

Dwil\GK15035

Drosophila virilis

Dvir\GJ18176

Drosophila mojavensis

Dmoj\GI12813

Drosophila grimshawi

Dgri\GH11139

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915004N )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17019364

Glossina morsitans

Tsetse fly

Gmor\GMOY004656

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_02569

Mayetiola destructor

Hessian fly

Mdes\Mdes011845

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001477

Anopheles darlingi

American malaria mosquito

Adar\ADAC008950

Anopheles gambiae

Malaria mosquito

Agam\AGAP007849

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004683

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W003O )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006098

Apis florea

Little honeybee

Aflo\2961247060

Apis mellifera

Western honey bee

Amel\GB53931

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25575

Bombus terrestris

Buff-tailed bumblebee

Bter\15207746

Linepithema humile

Argentine ant

Lhum\LH10455

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0020e9

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011291

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02834

Tribolium castaneum

Red flour beetle

Tcas\TC014773

Pediculus humanus

Human body louse

Phum\PHUM394240

Rhodnius prolixus

Kissing bug

Rpro\RPRC004257

Rhodnius prolixus

Kissing bug

Rpro\RPRC008582

Cimex lectularius

Bed bug

Clec\CLEC006984

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004055

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_03497

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X003E )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR010063

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78363.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78361.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM68921.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g03250

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_313559

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G005L )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Netrin1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Lama2L_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Lama2L

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Lamg1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Lama5Lf

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Lama2f

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-ProtglyL

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Net

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Lama2f_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-LamaLf_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-LamL

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_021166gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-LamaLf

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000004654

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007891

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007177

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005180

Gallus gallus

Domestic chicken

Ggal\LAMA5

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000029072

Gallus gallus

Domestic chicken

Ggal\LAMC1

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000015001

Gallus gallus

Domestic chicken

Ggal\LAMC3

Gallus gallus

Domestic chicken

Ggal\LAMC2

Gallus gallus

Domestic chicken

Ggal\Gga.55092

Gallus gallus

Domestic chicken

Ggal\LAMA1

Gallus gallus

Domestic chicken

Ggal\NTN1

Gallus gallus

Domestic chicken

Ggal\LAMA3

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (9)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

2 of 10

2 of 10

2 of 10

dsd

1 of 10

Megf8

1 of 10

SP2353

1 of 10

trol

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

LAMA2; laminin subunit alpha 2

model of congenital merosin-deficient muscular dystrophy 1A

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

LAMA1; laminin subunit alpha 1

10 of 15

150320

PORETTI-BOLTSHAUSER SYNDROME; PTBHS

LAMA2; laminin subunit alpha 2

9 of 15

156225

congenital merosin-deficient muscular dystrophy 1A

LAMA3; laminin subunit alpha 3

3 of 15

600805

LAMA5; laminin subunit alpha 5

3 of 15

601033

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

scb

(Schock and Perrimon, 2003)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Eogt|Dcr-2

enhanceable

(Müller et al., 2013)

mys

enhanceable

(Schock and Perrimon, 2003)

scb

enhanceable

(Schock and Perrimon, 2003)

sli

enhanceable

(MacMullin and Jacobs, 2006)

Smox

enhanceable

(Hevia et al., 2017)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261563

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43946

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

35A3-35A4

Sequence location

2L:14,263,027..14,328,262 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35A3-35A4

Limits computationally determined from genome sequence between P{lacW}l(2)34Fa^k00811&P{PZ}wb⁰⁹⁴³⁷ and P{EP}elB^EP2039&P{PZ}osp^rJ571

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

35A1-35A2

(Martin, 1999.3.16, BDGP Project Members, 1994-1999)

34F1-34F4

(determined by in situ hybridisation) 35A1--2 (determined by in situ hybridisation)

(Ashburner et al., 1999)

35A1-35A2

(determined by in situ hybridisation)

(Spradling et al., 1999)

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016, Ashburner et al., 1980)

2-46.1

(Comeron et al., 2012)

2-49.9

(Lindsley and Zimm, 1992)

Left of (cM)

(Ashburner et al., 1980)

Pgant35A

(Woodruff and Ashburner, 1979)

(Lindsley and Zimm, 1992, Woodruff and Ashburner, 1979)

Right of (cM)

Notes

Maps 0.1 cM to the left of Tp(2;2)Sco.

(Woodruff and Ashburner, 1979)

Stocks and Reagents

Stocks (34)

d03532

d03639

c02188

c03899

e00066

e00123

e02629

f03903

f06277

v102919

v108020

v3141

w¹¹¹⁸; P{GD1560}v3141

More stocks available...

Genomic Clones (46)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (20)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

MIP16024

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261563

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261563

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43946

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Martin et al., 1999)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: wb BEST:CK02229

(Ashburner et al., 1999)

Source for merge of: wb CG15288

(Misra, 2000.4.12)

Source for merge of: CG32970 BG:DS03792 .2

(FlyBase, 1996-)

Source for merge of: wb CG32970

(FlyBase Genome Annotators, 2010)

Additional comments

Annotations CG15288 and CG32970 merged as CG42677 in release 5.25 of the genome annotation.

(FlyBase Genome Annotators, 2010)

" BG:DS03792 .2" may correspond to "ms(2)34Fe".

(Ashburner et al., 1999.10.12)

Other Comments

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

(Inlow and Restifo, 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

New annotation (CG32970) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

wb is involved in processes requiring cell migration and cell adhesion.

(Martin et al., 1999)

wb has been characterised as a potential ligand of PS2 integrins by functional interaction in cell culture.

(Graner et al., 1998)

Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis.

(Kopczynski et al., 1998)

Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

(Roch et al., 1998)

One allele in this group has bent down wings when crossed to either the other lethals in the group or with deletions that included this locus.

(O'Donnell et al., 1977)

Most of the mutants are characterized by homo- or hemizygous lethality, but a visible phenotype has also been reported. O'Donnell et al., 1977 describe a bent-down-wing phenotype occurring when one of their lethals is crossed to other lethals in the same complementation group and the same phenotype when three of their lethals are crossed to deletions for the locus. Woodruff and Ashburner, 1979 describe the viable and fertile wing blister phenotype (a roughly circular area around the crossveins of the wing, forming a blister which often collapses soon after eclosion, distorting the wing blade). The allele showing this phenotype (wb¹) is viable and fertile in homozygotes and hemizygotes as well as in heteroallelic combinations with lethal alleles.

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 83 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43946

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261563

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

wb_1
wb_2

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CK02229

FlyMine - An integrated database for Drosophila genomics

FBgn0261563

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43946

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014773

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG42677

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

ECM-receptor

modMine - A data warehouse for the modENCODE project

FBgn0261563

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Laminin interactions

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261563

DRSC - Results frm RNAi screens

FBgn0261563

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0004002

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43946

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

ECM-receptor interaction

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Laminin interactions

Synonyms and Secondary IDs (36)

Reported As

Symbol Synonym

&agr1,2Lan

(Patel and Myat, 2013)

(Ashburner et al., 1980, O'Donnell et al., 1977)

BEST:CK02229

BG:DS03792.1

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

BG:DS03792.2

(Bayraktaroglu, 2000.4.8, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG15288

(Keleman et al., 2009.8.5, Perkins et al., 2009, Bhutkar et al., 2008, Ashburner, 2000.3.25)

CG32970

(Keleman et al., 2009.8.5)

CG42677

(Hevia et al., 2017, Hosono et al., 2015, FlyBase Genome Annotators, 2010)

CK02229

(Kopczynski et al., 1998)

CT35236

(Hynes and Zhao, 2000)

DLAM

(Graner et al., 1998)

LM-A/α2

(Hynes and Zhao, 2000)

LanA1,2

(Ismat et al., 2013)

(Zhang and Ten Hagen, 2011)

fus5

(Weyers et al., 2011)

l(2)09437

l(2)34Fb

(Ashburner et al., 1999, The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Alexandrova et al., 1996)

l(2)br1

(The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Gubb et al., 1985, Gubb et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982, Ashburner et al., 1980, Woodruff and Ashburner, 1979, Woodruff and Ashburner, 1979)

l34Fb

(Fossett et al., 1994)

(Ramos-Lewis and Page-McCaw, 2019, Wolfstetter et al., 2019, Joos et al., 2018, Lee et al., 2018, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, Ashwal-Fluss et al., 2014, Hollfelder et al., 2014, Kim and Choe, 2014, Pézeron et al., 2014, Ismat et al., 2013, Lindsley et al., 2013, Müller et al., 2013, Garelli et al., 2012, Han et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Manning et al., 2012, Vanderploeg et al., 2012, Wolfstetter and Holz, 2012, Carreira et al., 2011, Knox et al., 2011, Weyers et al., 2011, Sorrosal et al., 2010, Hazelett et al., 2009, Roote, 2009.11.24, Roote, 2009.11.24, Urbano et al., 2009, Bhutkar et al., 2008, Kwon et al., 2008, Tsai et al., 2008, Dietzl et al., 2007, Inoue and Hayashi, 2007, Morozova et al., 2007, MacMullin and Jacobs, 2006, Burgler and Macdonald, 2005)

α_1,2

(Urbano et al., 2009)

Name Synonyms

D-laminin α2

(Bunch et al., 1998, Graner et al., 1998)

Laminin A2

(Banyai and Patthy, 2004)

Laminin α1,2

(Brown et al., 2000)

LamininW

(Urbano et al., 2011)

SGP cluster fusion defects 5

(Weyers et al., 2011)

laminin

(Stevens and Jacobs, 2000)

laminin α1,2

(Brouzes and Farge, 2004, Martin et al., 1999)

laminin α1,2

(Urbano et al., 2009)

lamininW

(Knox et al., 2011)

lethal 1 in the black-reduced region

(Woodruff and Ashburner, 1979)

lethal group A1

(O'Donnell et al., 1977)

wing blister

(Wolfstetter et al., 2019, Han et al., 2012, Pool et al., 2012, Weyers et al., 2011, Sorrosal et al., 2010, Devenport et al., 2007, Inoue and Hayashi, 2007, Morozova et al., 2007, Martin, 1999.3.16)

wing blistered

(Volk, 1999)

wingblister

(Varshney and Stanley, 2017)

α laminin

(Inoue and Hayashi, 2007)

Secondary FlyBase IDs

FBgn0004002
FBgn0025691
FBgn0032544
FBgn0052970
FBgn0028885

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (158)

Report Sections

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