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Gene Dmel\eIF-2α

General Information
Symbol	Dmel\eIF-2α	Species	D. melanogaster
Name	eukaryotic translation Initiation Factor 2α	Annotation symbol	CG9946
Feature type	protein_coding_gene	FlyBase ID	FBgn0261609
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	eIF2α, eIF-2alpha, eIF-2, l(1)19-153, l(1)14Cf
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	14C6-14C6	Sequence location	X:16,339,209..16,341,026 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene eukaryotic translation Initiation Factor 2α is referred to in FlyBase by the symbol Dmel\eIF-2α (CG9946, FBgn0261609). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: translation initiation factor activity. There is experimental evidence that it is involved in the biological process: mitotic spindle elongation; mitotic spindle organization. 9 alleles are reported. The phenotype of these alleles is annotated with: macrochaeta. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Nucleic acid-binding, OB-fold; RNA-binding domain, S1; Ribosomal protein S1, RNA-binding domain; Translation initiation factor 2, alpha subunit; Translation initiation factor 2, alpha subunit, C-terminal; Translation initiation factor 2, alpha subunit, middle domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed within 00-18 hour embryonic stages, during early larval stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, adult crop, adult midgut, adult hindgut, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, larval midgut, larval hindgut, larval/adult Malpighian tubules, larval fat body, virgin spermatheca, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1632197_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of eIF-2alpha. Gene sequence location is X:16339528..16341007.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	M(1)14C Moderate Minute.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Polypeptides eIF-2alpha-PB Transcripts eIF-2alpha-RB
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 14C6-14C6   Limits computationally determined from genome sequence between P{EP}EP1547EP1547 and P{EP}CG4928EP1341; Left limit from inclusion within Tp(1;2)r+75c (FBrf0045066) Right limit from non-inclusion within Df(1)r-D1 (FBrf0045066)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 14C8-14D1   (determined by in situ hybridisation)   (Cavener, 1998.7.17)
Experimentally Determined Recombination Data
Location
Left of (cM)	r (Schalet, 1986)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\eIF-2α for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.51
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) eIF-2α-RA FBtr0074300  NM_132902   1200   341 eIF-2α-RB FBtr0343016     1519   341
Additional Transcript Data & Comments
Reported size (kB)	1.350 (northern blot) (Qu and Cavener, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein eIF-2α-PA   FBpp0074075   38.6   341   4.59     NP_573130   AAF48615 eIF-2α-PB   FBpp0309768   38.6   341   4.59
Additional Polypeptide Data & Comments
Reported size (kDa)	341 (aa) (Qu and Cavener, 1994)
Comments	eIF-2α protein is phosphorylated by Gcn2 protein. (Santoyo et al., 1997)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ribosomal protein S1, RNA-binding domain (IPR003029) Translation initiation factor 2, alpha subunit (IPR011488) Nucleic acid-binding, OB-fold (IPR012340) Translation initiation factor 2, alpha subunit, middle domain (IPR024054) Translation initiation factor 2, alpha subunit, C-terminal (IPR024055) RNA-binding domain, S1 (IPR022967)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390487   AA567301   AA695388   AA696176   AA802588   AA816954   AE014298 AAF48615   AF145633 AAD38608   AI106919   AI107987   AI114362   AI294430   AI295616   AI295761   AI386632   AI386970   AI402039   AI518348   BF489280   BF500195   BF500285   BF504014   BF504023   BG635870   BG638284   BG640539   BI171143   BI215411   BI242968   BI367284   BI371966   BI484258   BI484305   BI563872   BI565368   BI578274   BI580890   BI581339   BI581402   BI584415   BI612156   BI624016   BI625563   BI626566   BI635979   BI640015   BP548695   BP552774   BP552775   BX019409   BX020418   BX034246   BX042060   BX042247   BX045450   BX045477   BX046331   BX064182   BX065470   BX068435   CK659773   CO181670   CO262470   CO282643   CO283346   CO287232   CO291349   CO300093   CO315111   EC065540   EC195736   EC196561   EC199521   EC207931   EC233434   EC246035   L19196 AAA53627
UniProtKB/Swiss-Prot	P41374
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & larval stage     (Qu and Cavener, 1994)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, adult crop, adult midgut, adult hindgut, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, larval midgut, larval hindgut, larval/adult Malpighian tubules, larval fat body, virgin spermatheca, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    575.375 Larval Midgut    292.3 Larval Hindgut    476.5 Larval Malpighian Tubules    476.9 Larval Fat Body    495.1 Larval Salivary Gland    903.2 Larval Trachea    934.675 Larval Carcass    636.45 Adult Head    483.8 Adult Eye    636.625 Adult Brain    300.5 Adult Thoracic-Abdominal Ganglion    282 Adult Crop    521 Adult Midgut    552 Adult Hindgut    536.7 Adult Malpighian Tubules    427.3 Adult Fat Body    609.9 Adult Salivary Gland    696.3 Adult Heart    530.8 Adult VirginFemale Spermatheca    492.6 Adult InseminatedFemale Spermatheca    564 Adult Ovary    815.2 Adult Testis    347.2 Adult Male Accessory Gland    1029 Adult Carcass    500.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (575.375) Larval Midgut    292.3 Larval Hindgut    476.5 Larval Malpighian Tubules    476.9 Larval Fat Body    495.1 Larval Salivary Gland  (903.2) Larval Trachea  (934.675) Larval Carcass  (636.45) Adult Head    483.8 Adult Eye  (636.625) Adult Brain    300.5 Adult Thoracic-Abdominal Ganglion    282 Adult Crop  521 Adult Midgut  (552) Adult Hindgut  536.7 Adult Malpighian Tubules    427.3 Adult Fat Body  (609.9) Adult Salivary Gland  (696.3) Adult Heart  530.8 Adult VirginFemale Spermatheca    492.6 Adult InseminatedFemale Spermatheca  (564) Adult Ovary  (815.2) Adult Testis    347.2 Adult Male Accessory Gland  (1029) Adult Carcass  500.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    575.375 Larval Midgut    292.3 Larval Hindgut    476.5 Larval Malpighian Tubules    476.9 Larval Fat Body    495.1 Larval Salivary Gland    903.2 Larval Trachea    934.675 Larval Carcass    636.45 Adult Head    483.8 Adult Eye    636.625 Adult Brain    300.5 Adult Thoracic-Abdominal Ganglion    282 Adult Crop    521 Adult Midgut    552 Adult Hindgut    536.7 Adult Malpighian Tubules    427.3 Adult Fat Body    609.9 Adult Salivary Gland    696.3 Adult Heart    530.8 Adult VirginFemale Spermatheca    492.6 Adult InseminatedFemale Spermatheca    564 Adult Ovary    815.2 Adult Testis    347.2 Adult Male Accessory Gland  1029 Adult Carcass    500.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    575.375 Larval Midgut    292.3 Larval Hindgut    476.5 Larval Malpighian Tubules    476.9 Larval Fat Body    495.1 Larval Salivary Gland    903.2 Larval Trachea    934.675 Larval Carcass    636.45 Adult Head    483.8 Adult Eye    636.625 Adult Brain    300.5 Adult Thoracic-Abdominal Ganglion    282 Adult Crop    521 Adult Midgut    552 Adult Hindgut    536.7 Adult Malpighian Tubules    427.3 Adult Fat Body    609.9 Adult Salivary Gland    696.3 Adult Heart    530.8 Adult VirginFemale Spermatheca    492.6 Adult InseminatedFemale Spermatheca    564 Adult Ovary    815.2 Adult Testis    347.2 Adult Male Accessory Gland    1029 Adult Carcass    500.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    575.375 Larval Midgut    292.3 Larval Hindgut    476.5 Larval Malpighian Tubules    476.9 Larval Fat Body    495.1 Larval Salivary Gland    903.2 Larval Trachea    934.675 Larval Carcass    636.45 Adult Head    483.8 Adult Eye    636.625 Adult Brain    300.5 Adult Thoracic-Abdominal Ganglion    282 Adult Crop    521 Adult Midgut    552 Adult Hindgut    536.7 Adult Malpighian Tubules    427.3 Adult Fat Body    609.9 Adult Salivary Gland    696.3 Adult Heart    530.8 Adult VirginFemale Spermatheca    492.6 Adult InseminatedFemale Spermatheca    564 Adult Ovary    815.2 Adult Testis    347.2 Adult Male Accessory Gland    1029 Adult Carcass    500.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  575.375 Larval Midgut    292.3 Larval Hindgut    476.5 Larval Malpighian Tubules    476.9 Larval Fat Body    495.1 Larval Salivary Gland  (903.2) Larval Trachea  (934.675) Larval Carcass  636.45 Adult Head    483.8 Adult Eye  636.625 Adult Brain    300.5 Adult Thoracic-Abdominal Ganglion    282 Adult Crop  521 Adult Midgut  552 Adult Hindgut  536.7 Adult Malpighian Tubules    427.3 Adult Fat Body  609.9 Adult Salivary Gland  696.3 Adult Heart  530.8 Adult VirginFemale Spermatheca    492.6 Adult InseminatedFemale Spermatheca  564 Adult Ovary  (815.2) Adult Testis    347.2 Adult Male Accessory Gland  (1029) Adult Carcass  500.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    575.375 Larval Midgut    292.3 Larval Hindgut    476.5 Larval Malpighian Tubules    476.9 Larval Fat Body    495.1 Larval Salivary Gland    903.2 Larval Trachea    934.675 Larval Carcass    636.45 Adult Head    483.8 Adult Eye    636.625 Adult Brain    300.5 Adult Thoracic-Abdominal Ganglion    282 Adult Crop    521 Adult Midgut    552 Adult Hindgut    536.7 Adult Malpighian Tubules    427.3 Adult Fat Body    609.9 Adult Salivary Gland    696.3 Adult Heart    530.8 Adult VirginFemale Spermatheca    492.6 Adult InseminatedFemale Spermatheca    564 Adult Ovary    815.2 Adult Testis    347.2 Adult Male Accessory Gland  1029 Adult Carcass    500.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    575.375 Larval Midgut    292.3 Larval Hindgut    476.5 Larval Malpighian Tubules    476.9 Larval Fat Body    495.1 Larval Salivary Gland    903.2 Larval Trachea    934.675 Larval Carcass    636.45 Adult Head    483.8 Adult Eye    636.625 Adult Brain    300.5 Adult Thoracic-Abdominal Ganglion    282 Adult Crop    521 Adult Midgut    552 Adult Hindgut    536.7 Adult Malpighian Tubules    427.3 Adult Fat Body    609.9 Adult Salivary Gland    696.3 Adult Heart    530.8 Adult VirginFemale Spermatheca    492.6 Adult InseminatedFemale Spermatheca    564 Adult Ovary    815.2 Adult Testis    347.2 Adult Male Accessory Gland    1029 Adult Carcass    500.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    483.8   NA  Eye    636.625   NA  Brain    300.5   575.375  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    282   NA  Crop    521   292.3  Midgut    552   476.5  Hindgut    536.7   476.9  Malpighian Tubules    427.3   495.1  Fat Body    609.9   903.2  Salivary Gland    696.3   NA  Heart    530.8   934.675  Trachea    NA   NA  VirginFemale Spermatheca    492.6   NA  InseminatedFemale Spermatheca    564   NA  Ovary    815.2   NA  Testis    347.2   NA  Male Accessory Gland    1029   636.45  Carcass    500.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    129 central nervous system, larvae L3    100 central nervous system, pupae P8    38 head, virgin 1-day female    48 head, virgin 4-day female    61 head, virgin 20-day female    43 head, mated 1-day female    45 head, mated 4-day female    54 head, mated 20-day female    42 head, mated 1-day male    41 head, mated 4-day male    45 head, mated 20-day male    47 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    34 digestive system, larvae L3 wandering    57 digestive system, 1-day adult    48 digestive system, 4-day adult    60 digestive system, 20-day adult    50 fat body, larvae L3 wandering    57 fat body, white prepupae    41 fat body, pupae P8    84 carcass, larvae L3 wandering    101 carcass, 1-day adult    48 carcass, 4-day adult    62 carcass, 20-day adult    57 ovary, virgin 4-day female    111 ovary, mated 4-day female    144 testis, mated 4-day male    104 accessory gland, mated 4-day male    113 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (129) central nervous system, larvae L3  (100) central nervous system, pupae P8  (38) head, virgin 1-day female  (48) head, virgin 4-day female  (61) head, virgin 20-day female  (43) head, mated 1-day female  (45) head, mated 4-day female  (54) head, mated 20-day female  (42) head, mated 1-day male  (41) head, mated 4-day male  (45) head, mated 20-day male  (47) salivary gland, larvae L3 wandering  (36) salivary gland, white prepupae  (34) digestive system, larvae L3 wandering  (57) digestive system, 1-day adult  (48) digestive system, 4-day adult  (60) digestive system, 20-day adult  (50) fat body, larvae L3 wandering  (57) fat body, white prepupae  (41) fat body, pupae P8  (84) carcass, larvae L3 wandering  (101) carcass, 1-day adult  (48) carcass, 4-day adult  (62) carcass, 20-day adult  (57) ovary, virgin 4-day female  (111) ovary, mated 4-day female  (144) testis, mated 4-day male  (104) accessory gland, mated 4-day male  (113) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (129) central nervous system, larvae L3    100 central nervous system, pupae P8    38 head, virgin 1-day female    48 head, virgin 4-day female    61 head, virgin 20-day female    43 head, mated 1-day female    45 head, mated 4-day female    54 head, mated 20-day female    42 head, mated 1-day male    41 head, mated 4-day male    45 head, mated 20-day male    47 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    34 digestive system, larvae L3 wandering    57 digestive system, 1-day adult    48 digestive system, 4-day adult    60 digestive system, 20-day adult    50 fat body, larvae L3 wandering    57 fat body, white prepupae    41 fat body, pupae P8    84 carcass, larvae L3 wandering  101 carcass, 1-day adult    48 carcass, 4-day adult    62 carcass, 20-day adult    57 ovary, virgin 4-day female  (111) ovary, mated 4-day female  (144) testis, mated 4-day male  104 accessory gland, mated 4-day male  (113) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    129 central nervous system, larvae L3    100 central nervous system, pupae P8    38 head, virgin 1-day female    48 head, virgin 4-day female    61 head, virgin 20-day female    43 head, mated 1-day female    45 head, mated 4-day female    54 head, mated 20-day female    42 head, mated 1-day male    41 head, mated 4-day male    45 head, mated 20-day male    47 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    34 digestive system, larvae L3 wandering    57 digestive system, 1-day adult    48 digestive system, 4-day adult    60 digestive system, 20-day adult    50 fat body, larvae L3 wandering    57 fat body, white prepupae    41 fat body, pupae P8    84 carcass, larvae L3 wandering    101 carcass, 1-day adult    48 carcass, 4-day adult    62 carcass, 20-day adult    57 ovary, virgin 4-day female    111 ovary, mated 4-day female    144 testis, mated 4-day male    104 accessory gland, mated 4-day male    113 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    129 central nervous system, larvae L3    100 central nervous system, pupae P8    38 head, virgin 1-day female    48 head, virgin 4-day female    61 head, virgin 20-day female    43 head, mated 1-day female    45 head, mated 4-day female    54 head, mated 20-day female    42 head, mated 1-day male    41 head, mated 4-day male    45 head, mated 20-day male    47 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    34 digestive system, larvae L3 wandering    57 digestive system, 1-day adult    48 digestive system, 4-day adult    60 digestive system, 20-day adult    50 fat body, larvae L3 wandering    57 fat body, white prepupae    41 fat body, pupae P8    84 carcass, larvae L3 wandering    101 carcass, 1-day adult    48 carcass, 4-day adult    62 carcass, 20-day adult    57 ovary, virgin 4-day female    111 ovary, mated 4-day female    144 testis, mated 4-day male    104 accessory gland, mated 4-day male    113 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (129) central nervous system, larvae L3  (100) central nervous system, pupae P8  (38) head, virgin 1-day female  (48) head, virgin 4-day female  (61) head, virgin 20-day female  (43) head, mated 1-day female  (45) head, mated 4-day female  (54) head, mated 20-day female  (42) head, mated 1-day male  (41) head, mated 4-day male  (45) head, mated 20-day male  (47) salivary gland, larvae L3 wandering  (36) salivary gland, white prepupae  (34) digestive system, larvae L3 wandering  (57) digestive system, 1-day adult  (48) digestive system, 4-day adult  (60) digestive system, 20-day adult  (50) fat body, larvae L3 wandering  (57) fat body, white prepupae  (41) fat body, pupae P8  (84) carcass, larvae L3 wandering  (101) carcass, 1-day adult  (48) carcass, 4-day adult  (62) carcass, 20-day adult  (57) ovary, virgin 4-day female  (111) ovary, mated 4-day female  (144) testis, mated 4-day male  (104) accessory gland, mated 4-day male  (113) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  129 central nervous system, larvae L3    100 central nervous system, pupae P8    38 head, virgin 1-day female    48 head, virgin 4-day female    61 head, virgin 20-day female    43 head, mated 1-day female    45 head, mated 4-day female    54 head, mated 20-day female    42 head, mated 1-day male    41 head, mated 4-day male    45 head, mated 20-day male    47 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    34 digestive system, larvae L3 wandering    57 digestive system, 1-day adult    48 digestive system, 4-day adult    60 digestive system, 20-day adult    50 fat body, larvae L3 wandering    57 fat body, white prepupae    41 fat body, pupae P8    84 carcass, larvae L3 wandering  101 carcass, 1-day adult    48 carcass, 4-day adult    62 carcass, 20-day adult    57 ovary, virgin 4-day female  111 ovary, mated 4-day female  (144) testis, mated 4-day male  104 accessory gland, mated 4-day male  113 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    129 central nervous system, larvae L3    100 central nervous system, pupae P8    38 head, virgin 1-day female    48 head, virgin 4-day female    61 head, virgin 20-day female    43 head, mated 1-day female    45 head, mated 4-day female    54 head, mated 20-day female    42 head, mated 1-day male    41 head, mated 4-day male    45 head, mated 20-day male    47 salivary gland, larvae L3 wandering    36 salivary gland, white prepupae    34 digestive system, larvae L3 wandering    57 digestive system, 1-day adult    48 digestive system, 4-day adult    60 digestive system, 20-day adult    50 fat body, larvae L3 wandering    57 fat body, white prepupae    41 fat body, pupae P8    84 carcass, larvae L3 wandering    101 carcass, 1-day adult    48 carcass, 4-day adult    62 carcass, 20-day adult    57 ovary, virgin 4-day female    111 ovary, mated 4-day female    144 testis, mated 4-day male    104 accessory gland, mated 4-day male    113 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed within 00-18 hour embryonic stages, during early larval stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    189 embryo 02-04hr    111 embryo 04-06hr    131 embryo 06-08hr    176 embryo 08-10hr    177 embryo 10-12hr    215 embryo 12-14hr    127 embryo 14-16hr    134 embryo 16-18hr    98 embryo 18-20hr    90 embryo 20-22hr    97 embryo 22-24hr    76 larva L1    212 larva L2    194 larva L3 12hr old    144 larva L3 puffstage 1-2    92 larva L3 puffstage 3-6    102 larva L3 puffstage 7-9    101 white prepupae new    95 white prepupae 12hr    81 white prepupae 24hr    92 pupae 2d postWPP    86 pupae 3d postWPP    62 pupae 4d postWPP    72 adult male 01day    75 adult male 05day    87 adult male 30day    86 adult female 01day    170 adult female 05day    172 adult female 30day    187 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (189) embryo 02-04hr  (111) embryo 04-06hr  (131) embryo 06-08hr  (176) embryo 08-10hr  (177) embryo 10-12hr  (215) embryo 12-14hr  (127) embryo 14-16hr  (134) embryo 16-18hr  (98) embryo 18-20hr  (90) embryo 20-22hr  (97) embryo 22-24hr  (76) larva L1  (212) larva L2  (194) larva L3 12hr old  (144) larva L3 puffstage 1-2  (92) larva L3 puffstage 3-6  (102) larva L3 puffstage 7-9  (101) white prepupae new  (95) white prepupae 12hr  (81) white prepupae 24hr  (92) pupae 2d postWPP  (86) pupae 3d postWPP  (62) pupae 4d postWPP  (72) adult male 01day  (75) adult male 05day  (87) adult male 30day  (86) adult female 01day  (170) adult female 05day  (172) adult female 30day  (187) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (189) embryo 02-04hr  (111) embryo 04-06hr  (131) embryo 06-08hr  (176) embryo 08-10hr  (177) embryo 10-12hr  (215) embryo 12-14hr  (127) embryo 14-16hr  (134) embryo 16-18hr    98 embryo 18-20hr    90 embryo 20-22hr    97 embryo 22-24hr    76 larva L1  (212) larva L2  (194) larva L3 12hr old  (144) larva L3 puffstage 1-2    92 larva L3 puffstage 3-6  102 larva L3 puffstage 7-9  101 white prepupae new    95 white prepupae 12hr    81 white prepupae 24hr    92 pupae 2d postWPP    86 pupae 3d postWPP    62 pupae 4d postWPP    72 adult male 01day    75 adult male 05day    87 adult male 30day    86 adult female 01day  (170) adult female 05day  (172) adult female 30day  (187) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    189 embryo 02-04hr    111 embryo 04-06hr    131 embryo 06-08hr    176 embryo 08-10hr    177 embryo 10-12hr    215 embryo 12-14hr    127 embryo 14-16hr    134 embryo 16-18hr    98 embryo 18-20hr    90 embryo 20-22hr    97 embryo 22-24hr    76 larva L1    212 larva L2    194 larva L3 12hr old    144 larva L3 puffstage 1-2    92 larva L3 puffstage 3-6    102 larva L3 puffstage 7-9    101 white prepupae new    95 white prepupae 12hr    81 white prepupae 24hr    92 pupae 2d postWPP    86 pupae 3d postWPP    62 pupae 4d postWPP    72 adult male 01day    75 adult male 05day    87 adult male 30day    86 adult female 01day    170 adult female 05day    172 adult female 30day    187 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    189 embryo 02-04hr    111 embryo 04-06hr    131 embryo 06-08hr    176 embryo 08-10hr    177 embryo 10-12hr    215 embryo 12-14hr    127 embryo 14-16hr    134 embryo 16-18hr    98 embryo 18-20hr    90 embryo 20-22hr    97 embryo 22-24hr    76 larva L1    212 larva L2    194 larva L3 12hr old    144 larva L3 puffstage 1-2    92 larva L3 puffstage 3-6    102 larva L3 puffstage 7-9    101 white prepupae new    95 white prepupae 12hr    81 white prepupae 24hr    92 pupae 2d postWPP    86 pupae 3d postWPP    62 pupae 4d postWPP    72 adult male 01day    75 adult male 05day    87 adult male 30day    86 adult female 01day    170 adult female 05day    172 adult female 30day    187 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (189) embryo 02-04hr  (111) embryo 04-06hr  (131) embryo 06-08hr  (176) embryo 08-10hr  (177) embryo 10-12hr  (215) embryo 12-14hr  (127) embryo 14-16hr  (134) embryo 16-18hr  (98) embryo 18-20hr  (90) embryo 20-22hr  (97) embryo 22-24hr  (76) larva L1  (212) larva L2  (194) larva L3 12hr old  (144) larva L3 puffstage 1-2  (92) larva L3 puffstage 3-6  (102) larva L3 puffstage 7-9  (101) white prepupae new  (95) white prepupae 12hr  (81) white prepupae 24hr  (92) pupae 2d postWPP  (86) pupae 3d postWPP  (62) pupae 4d postWPP  (72) adult male 01day  (75) adult male 05day  (87) adult male 30day  (86) adult female 01day  (170) adult female 05day  (172) adult female 30day  (187) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (189) embryo 02-04hr  111 embryo 04-06hr  131 embryo 06-08hr  (176) embryo 08-10hr  (177) embryo 10-12hr  (215) embryo 12-14hr  127 embryo 14-16hr  134 embryo 16-18hr    98 embryo 18-20hr    90 embryo 20-22hr    97 embryo 22-24hr    76 larva L1  (212) larva L2  (194) larva L3 12hr old  (144) larva L3 puffstage 1-2    92 larva L3 puffstage 3-6  102 larva L3 puffstage 7-9  101 white prepupae new    95 white prepupae 12hr    81 white prepupae 24hr    92 pupae 2d postWPP    86 pupae 3d postWPP    62 pupae 4d postWPP    72 adult male 01day    75 adult male 05day    87 adult male 30day    86 adult female 01day  (170) adult female 05day  (172) adult female 30day  (187) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    189 embryo 02-04hr    111 embryo 04-06hr    131 embryo 06-08hr    176 embryo 08-10hr    177 embryo 10-12hr    215 embryo 12-14hr    127 embryo 14-16hr    134 embryo 16-18hr    98 embryo 18-20hr    90 embryo 20-22hr    97 embryo 22-24hr    76 larva L1    212 larva L2    194 larva L3 12hr old    144 larva L3 puffstage 1-2    92 larva L3 puffstage 3-6    102 larva L3 puffstage 7-9    101 white prepupae new    95 white prepupae 12hr    81 white prepupae 24hr    92 pupae 2d postWPP    86 pupae 3d postWPP    62 pupae 4d postWPP    72 adult male 01day    75 adult male 05day    87 adult male 30day    86 adult female 01day    170 adult female 05day    172 adult female 30day    187 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    393 Schneider line 2 Sg4    222 embryonic 1182-4H    217 embryonic GM2    145 embryonic Kc167    209 embryonic S1    158 embryonic S3    160 leg disc CME L1    269 wing disc CME-W2    135 wing disc ML-DmD8    121 wing disc ML-DmD9    176 wing disc ML-DmD16-c3    253 wing disc ML-DmD21    325 wing disc ML-DmD32    183 haltere disc ML-DmD17-c3    108 eye-antennal disc ML-DmD11    230 antennal disc ML-DmD20-c5    208 mixed discs ML-DmD4-c1    196 CNS ML-DmBG1-c1    220 CNS ML-DmBG2-c2    171 tumorous blood cells mbn2    116 ovary fGS/OSS    123 ovary OSC    92 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (393) Schneider line 2 Sg4  (222) embryonic 1182-4H  (217) embryonic GM2  (145) embryonic Kc167  (209) embryonic S1  (158) embryonic S3  (160) leg disc CME L1  (269) wing disc CME-W2  (135) wing disc ML-DmD8  (121) wing disc ML-DmD9  (176) wing disc ML-DmD16-c3  (253) wing disc ML-DmD21  (325) wing disc ML-DmD32  (183) haltere disc ML-DmD17-c3  (108) eye-antennal disc ML-DmD11  (230) antennal disc ML-DmD20-c5  (208) mixed discs ML-DmD4-c1  (196) CNS ML-DmBG1-c1  (220) CNS ML-DmBG2-c2  (171) tumorous blood cells mbn2  (116) ovary fGS/OSS  (123) ovary OSC  (92) ovary OSS  (37) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (393) Schneider line 2 Sg4  (222) embryonic 1182-4H  (217) embryonic GM2  (145) embryonic Kc167  (209) embryonic S1  (158) embryonic S3  (160) leg disc CME L1  (269) wing disc CME-W2  (135) wing disc ML-DmD8  (121) wing disc ML-DmD9  (176) wing disc ML-DmD16-c3  (253) wing disc ML-DmD21  (325) wing disc ML-DmD32  (183) haltere disc ML-DmD17-c3  (108) eye-antennal disc ML-DmD11  (230) antennal disc ML-DmD20-c5  (208) mixed discs ML-DmD4-c1  (196) CNS ML-DmBG1-c1  (220) CNS ML-DmBG2-c2  (171) tumorous blood cells mbn2  (116) ovary fGS/OSS  (123) ovary OSC    92 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    393 Schneider line 2 Sg4    222 embryonic 1182-4H    217 embryonic GM2    145 embryonic Kc167    209 embryonic S1    158 embryonic S3    160 leg disc CME L1    269 wing disc CME-W2    135 wing disc ML-DmD8    121 wing disc ML-DmD9    176 wing disc ML-DmD16-c3    253 wing disc ML-DmD21    325 wing disc ML-DmD32    183 haltere disc ML-DmD17-c3    108 eye-antennal disc ML-DmD11    230 antennal disc ML-DmD20-c5    208 mixed discs ML-DmD4-c1    196 CNS ML-DmBG1-c1    220 CNS ML-DmBG2-c2    171 tumorous blood cells mbn2    116 ovary fGS/OSS    123 ovary OSC    92 ovary OSS    37 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    393 Schneider line 2 Sg4    222 embryonic 1182-4H    217 embryonic GM2    145 embryonic Kc167    209 embryonic S1    158 embryonic S3    160 leg disc CME L1    269 wing disc CME-W2    135 wing disc ML-DmD8    121 wing disc ML-DmD9    176 wing disc ML-DmD16-c3    253 wing disc ML-DmD21    325 wing disc ML-DmD32    183 haltere disc ML-DmD17-c3    108 eye-antennal disc ML-DmD11    230 antennal disc ML-DmD20-c5    208 mixed discs ML-DmD4-c1    196 CNS ML-DmBG1-c1    220 CNS ML-DmBG2-c2    171 tumorous blood cells mbn2    116 ovary fGS/OSS    123 ovary OSC    92 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (393) Schneider line 2 Sg4  (222) embryonic 1182-4H  (217) embryonic GM2  (145) embryonic Kc167  (209) embryonic S1  (158) embryonic S3  (160) leg disc CME L1  (269) wing disc CME-W2  (135) wing disc ML-DmD8  (121) wing disc ML-DmD9  (176) wing disc ML-DmD16-c3  (253) wing disc ML-DmD21  (325) wing disc ML-DmD32  (183) haltere disc ML-DmD17-c3  (108) eye-antennal disc ML-DmD11  (230) antennal disc ML-DmD20-c5  (208) mixed discs ML-DmD4-c1  (196) CNS ML-DmBG1-c1  (220) CNS ML-DmBG2-c2  (171) tumorous blood cells mbn2  (116) ovary fGS/OSS  (123) ovary OSC  (92) ovary OSS  (37) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (393) Schneider line 2 Sg4  (222) embryonic 1182-4H  (217) embryonic GM2  (145) embryonic Kc167  (209) embryonic S1  (158) embryonic S3  (160) leg disc CME L1  (269) wing disc CME-W2  135 wing disc ML-DmD8  121 wing disc ML-DmD9  (176) wing disc ML-DmD16-c3  (253) wing disc ML-DmD21  (325) wing disc ML-DmD32  (183) haltere disc ML-DmD17-c3  108 eye-antennal disc ML-DmD11  (230) antennal disc ML-DmD20-c5  (208) mixed discs ML-DmD4-c1  (196) CNS ML-DmBG1-c1  (220) CNS ML-DmBG2-c2  (171) tumorous blood cells mbn2  116 ovary fGS/OSS  123 ovary OSC    92 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    393 Schneider line 2 Sg4    222 embryonic 1182-4H    217 embryonic GM2    145 embryonic Kc167    209 embryonic S1    158 embryonic S3    160 leg disc CME L1    269 wing disc CME-W2    135 wing disc ML-DmD8    121 wing disc ML-DmD9    176 wing disc ML-DmD16-c3    253 wing disc ML-DmD21    325 wing disc ML-DmD32    183 haltere disc ML-DmD17-c3    108 eye-antennal disc ML-DmD11    230 antennal disc ML-DmD20-c5    208 mixed discs ML-DmD4-c1    196 CNS ML-DmBG1-c1    220 CNS ML-DmBG2-c2    171 tumorous blood cells mbn2    116 ovary fGS/OSS    123 ovary OSC    92 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    119 cold shock, 4-day adult    84 heat shock, 4-day adult    101 Cadmium 50 mM 6 hrs, larvae L3    86 Cadmium 50 mM 12 hrs, larvae L3    61 Cadmium 50 mM 48 hrs, 4-day adult    87 Cadmium 100 mM 48 hrs, 4-day adult    124 Copper 0.5 mM 12 hrs, larvae L3    168 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    107 Zinc 4.5 mM 48 hrs, 4-day adult    68 Ethanol 2.5% 3 hrs, larvae L3    134 Ethanol 5% 3 hrs, larvae L3    76 Ethanol 10% 3 hrs, larvae L3    86 Caffeine 1.5 mg/ml 4 hrs, larvae L3    64 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    119 Caffeine 25 mg/ml 48 hrs, 4-day adult    97 Paraquat 5 mM 48 hrs, 4-day adult    121 Paraquat 10 mM 48 hrs, 4-day adult    126 Rotenone 2 μg 12 hrs, larvae L3    79 Rotenone 8 μg 12 hrs, larvae L3    101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (119) cold shock, 4-day adult  (84) heat shock, 4-day adult  (101) Cadmium 50 mM 6 hrs, larvae L3  (86) Cadmium 50 mM 12 hrs, larvae L3  (61) Cadmium 50 mM 48 hrs, 4-day adult  (87) Cadmium 100 mM 48 hrs, 4-day adult  (124) Copper 0.5 mM 12 hrs, larvae L3  (168) Copper 15 mM 48 hrs, 4-day adult  (61) Zinc 5 mM 12 hrs, larvae L3  (107) Zinc 4.5 mM 48 hrs, 4-day adult  (68) Ethanol 2.5% 3 hrs, larvae L3  (134) Ethanol 5% 3 hrs, larvae L3  (76) Ethanol 10% 3 hrs, larvae L3  (86) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (64) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (119) Caffeine 25 mg/ml 48 hrs, 4-day adult  (97) Paraquat 5 mM 48 hrs, 4-day adult  (121) Paraquat 10 mM 48 hrs, 4-day adult  (126) Rotenone 2 μg 12 hrs, larvae L3  (79) Rotenone 8 μg 12 hrs, larvae L3  (101) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (119) cold shock, 4-day adult    84 heat shock, 4-day adult  101 Cadmium 50 mM 6 hrs, larvae L3    86 Cadmium 50 mM 12 hrs, larvae L3    61 Cadmium 50 mM 48 hrs, 4-day adult    87 Cadmium 100 mM 48 hrs, 4-day adult  (124) Copper 0.5 mM 12 hrs, larvae L3  (168) Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3  107 Zinc 4.5 mM 48 hrs, 4-day adult    68 Ethanol 2.5% 3 hrs, larvae L3  (134) Ethanol 5% 3 hrs, larvae L3    76 Ethanol 10% 3 hrs, larvae L3    86 Caffeine 1.5 mg/ml 4 hrs, larvae L3    64 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (119) Caffeine 25 mg/ml 48 hrs, 4-day adult    97 Paraquat 5 mM 48 hrs, 4-day adult  (121) Paraquat 10 mM 48 hrs, 4-day adult  (126) Rotenone 2 μg 12 hrs, larvae L3    79 Rotenone 8 μg 12 hrs, larvae L3  101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    119 cold shock, 4-day adult    84 heat shock, 4-day adult    101 Cadmium 50 mM 6 hrs, larvae L3    86 Cadmium 50 mM 12 hrs, larvae L3    61 Cadmium 50 mM 48 hrs, 4-day adult    87 Cadmium 100 mM 48 hrs, 4-day adult    124 Copper 0.5 mM 12 hrs, larvae L3    168 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    107 Zinc 4.5 mM 48 hrs, 4-day adult    68 Ethanol 2.5% 3 hrs, larvae L3    134 Ethanol 5% 3 hrs, larvae L3    76 Ethanol 10% 3 hrs, larvae L3    86 Caffeine 1.5 mg/ml 4 hrs, larvae L3    64 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    119 Caffeine 25 mg/ml 48 hrs, 4-day adult    97 Paraquat 5 mM 48 hrs, 4-day adult    121 Paraquat 10 mM 48 hrs, 4-day adult    126 Rotenone 2 μg 12 hrs, larvae L3    79 Rotenone 8 μg 12 hrs, larvae L3    101 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    119 cold shock, 4-day adult    84 heat shock, 4-day adult    101 Cadmium 50 mM 6 hrs, larvae L3    86 Cadmium 50 mM 12 hrs, larvae L3    61 Cadmium 50 mM 48 hrs, 4-day adult    87 Cadmium 100 mM 48 hrs, 4-day adult    124 Copper 0.5 mM 12 hrs, larvae L3    168 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    107 Zinc 4.5 mM 48 hrs, 4-day adult    68 Ethanol 2.5% 3 hrs, larvae L3    134 Ethanol 5% 3 hrs, larvae L3    76 Ethanol 10% 3 hrs, larvae L3    86 Caffeine 1.5 mg/ml 4 hrs, larvae L3    64 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    119 Caffeine 25 mg/ml 48 hrs, 4-day adult    97 Paraquat 5 mM 48 hrs, 4-day adult    121 Paraquat 10 mM 48 hrs, 4-day adult    126 Rotenone 2 μg 12 hrs, larvae L3    79 Rotenone 8 μg 12 hrs, larvae L3    101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (119) cold shock, 4-day adult  (84) heat shock, 4-day adult  (101) Cadmium 50 mM 6 hrs, larvae L3  (86) Cadmium 50 mM 12 hrs, larvae L3  (61) Cadmium 50 mM 48 hrs, 4-day adult  (87) Cadmium 100 mM 48 hrs, 4-day adult  (124) Copper 0.5 mM 12 hrs, larvae L3  (168) Copper 15 mM 48 hrs, 4-day adult  (61) Zinc 5 mM 12 hrs, larvae L3  (107) Zinc 4.5 mM 48 hrs, 4-day adult  (68) Ethanol 2.5% 3 hrs, larvae L3  (134) Ethanol 5% 3 hrs, larvae L3  (76) Ethanol 10% 3 hrs, larvae L3  (86) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (64) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (119) Caffeine 25 mg/ml 48 hrs, 4-day adult  (97) Paraquat 5 mM 48 hrs, 4-day adult  (121) Paraquat 10 mM 48 hrs, 4-day adult  (126) Rotenone 2 μg 12 hrs, larvae L3  (79) Rotenone 8 μg 12 hrs, larvae L3  (101) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  119 cold shock, 4-day adult    84 heat shock, 4-day adult  101 Cadmium 50 mM 6 hrs, larvae L3    86 Cadmium 50 mM 12 hrs, larvae L3    61 Cadmium 50 mM 48 hrs, 4-day adult    87 Cadmium 100 mM 48 hrs, 4-day adult  124 Copper 0.5 mM 12 hrs, larvae L3  (168) Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3  107 Zinc 4.5 mM 48 hrs, 4-day adult    68 Ethanol 2.5% 3 hrs, larvae L3  134 Ethanol 5% 3 hrs, larvae L3    76 Ethanol 10% 3 hrs, larvae L3    86 Caffeine 1.5 mg/ml 4 hrs, larvae L3    64 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  119 Caffeine 25 mg/ml 48 hrs, 4-day adult    97 Paraquat 5 mM 48 hrs, 4-day adult  121 Paraquat 10 mM 48 hrs, 4-day adult  126 Rotenone 2 μg 12 hrs, larvae L3    79 Rotenone 8 μg 12 hrs, larvae L3  101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    119 cold shock, 4-day adult    84 heat shock, 4-day adult    101 Cadmium 50 mM 6 hrs, larvae L3    86 Cadmium 50 mM 12 hrs, larvae L3    61 Cadmium 50 mM 48 hrs, 4-day adult    87 Cadmium 100 mM 48 hrs, 4-day adult    124 Copper 0.5 mM 12 hrs, larvae L3    168 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    107 Zinc 4.5 mM 48 hrs, 4-day adult    68 Ethanol 2.5% 3 hrs, larvae L3    134 Ethanol 5% 3 hrs, larvae L3    76 Ethanol 10% 3 hrs, larvae L3    86 Caffeine 1.5 mg/ml 4 hrs, larvae L3    64 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    119 Caffeine 25 mg/ml 48 hrs, 4-day adult    97 Paraquat 5 mM 48 hrs, 4-day adult    121 Paraquat 10 mM 48 hrs, 4-day adult    126 Rotenone 2 μg 12 hrs, larvae L3    79 Rotenone 8 μg 12 hrs, larvae L3    101 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004925
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 eIF-2αGD1430 lethal | heat sensitive eIF-2αHD.hs lethal | larval stage | recessive eIF-2αX1 lethal | pupal stage, with Scer\GAL4pnr-MD237 eIF-2αGD1430 Other Phenotypes Allele Minute eIF-2α5-HA-1790 eIF-2α815-29 Minute | dominant eIF-2α1 visible eIF-2α1 visible | dominant eIF-2α1 Phenotype manifest in Allele macrochaeta eIF-2α1
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of eIF-2α Class Mutagen Stocks Known lesion eIF-2α815-29 X ray 2 -- eIF-2α1 spontaneous 0 -- eIF-2α5-HA-1790 0 -- eIF-2αX1 X ray 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of eIF-2α Class Mutagen Stocks Known lesion eIF-2αGD1430 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes eIF-2αKK100282 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes eIF-2αHA.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes eIF-2αHD.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes eIF-2αhs.PQ in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)ED7364 (Marygold et al., 2007) Df(1)FDD-0230908 (Marygold et al., 2007) Df(1)l32 (Banga et al., 1995) Df(1)r75c (Schalet, 1986)
Duplicated in	Dp(1;1)FDDP-0024486 (Marygold et al., 2007) Dp(1;2)r+75c (Banga et al., 1995, Schalet, 1986)
Not disrupted in	Df(1)r-D1 (Banga et al., 1995, Schalet, 1986) Df(1)rif (Brown, 1994)
Not duplicated in	Dp(1;4)80f18c (Banga et al., 1995) Dp(1;4)81l8g (Banga et al., 1995) Dp(1;4)81l12h (Banga et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-eIF-2α.HA} NA P{hs-eIF-2α.HD} NA P{hs-eIF-2α.HS} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{RS5}eIF-2α5-HA-1790 NA insertion of enhancer trap P{lacW}l(1)G0272G0272 No
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
translation initiation factor activity inferred from genetic interaction with Saccharomyces GCN3 (Williams et al., 2001)
Biological Process
CV term Evidence References
mitotic spindle elongation inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
GTP binding non-traceable author statement (Swiss-Prot Project Members, 1995.6.1) translation initiation factor activity inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with human EIF2S1 AND inferred from sequence or structural similarity with Saccharomyces SUI2 (Qu and Cavener, 1994) inferred from sequence or structural similarity with Saccharomyces GCN3 (Williams et al., 2001) non-traceable author statement (Swiss-Prot Project Members, 1995.6.1) tRNA binding non-traceable author statement (Swiss-Prot Project Members, 1995.6.1)
Biological Process
CV term Evidence References
formation of translation initiation ternary complex non-traceable author statement (Swiss-Prot Project Members, 1995.6.1) translational initiation inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with human EIF2S1 AND inferred from sequence or structural similarity with Saccharomyces SUI2 (Qu and Cavener, 1994) inferred from sequence or structural similarity with Saccharomyces GCN3 (Williams et al., 2001)
Cellular Component
CV term Evidence References
cytosol inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with human EIF2S1 AND inferred from sequence or structural similarity with Saccharomyces SUI2 (Qu and Cavener, 1994) eukaryotic translation initiation factor 2B complex inferred from sequence or structural similarity with Saccharomyces GCN3 (Williams et al., 2001) eukaryotic translation initiation factor 2 complex inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with human EIF2S1 AND inferred from sequence or structural similarity with Saccharomyces SUI2 (Qu and Cavener, 1994) non-traceable author statement (Swiss-Prot Project Members, 1995.6.1)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
eIF-2α - Art7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Bap55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - BicD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG12163 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG12264 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG1701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG1785 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG32344 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG3436 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG3756 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG3862 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG4612 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG6937 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG8043 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG8478 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - CG8545 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Cbp80 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Cp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Dp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Droj2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Eb1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Map205 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Mi-2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Mtpα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Nacα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - P32 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - PEK enzymatic study, autoradiography (Pomar et al., 2003) eIF-2α - Prp3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - RpS19a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - RpS9 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Rs1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - SC35 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - SRPK anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Sec61β anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - SmD3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Srp68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Thiolase anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Tom20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Top3β anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Upf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - eIF-2β anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - fs(1)K10 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - hts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - ligatin anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - mRpL13 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - mRpL2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - mRpL39 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - mRpS33 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - mod(mdg4) anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - mtg experimental knowledge based (Guruharsha et al., 2011) eIF-2α - nito anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - pav anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - qkr54B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - qkr58E-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - skpA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - α-Spec anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) eIF-2α - εCOP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions eIF-2α allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261609 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32617
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG63JCQT Dipteran inclusive ortholog search EOG67SSPM Insect inclusive ortholog search EOG67SR9M Arthropod inclusive ortholog search EOG65MKN4 Metazoa inclusive ortholog search EOG6996TR
Orthologs in Drosophila Species (EOG63JCQT)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\eIF-2α     Drosophila simulans   Dsim\GD15749 Y   Drosophila sechellia   Dsec\GM13399 Y   Drosophila erecta   Dere\GG19321 Y   Drosophila yakuba   Dyak\GE15968 Y   Drosophila ananassae   Dana\GF19374 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA22144 Y   Drosophila persimilis   Dper\GL20268 Y   Drosophila willistoni   Dwil\GK25248 Y   Drosophila virilis   Dvir\GJ19141 Y   Drosophila mojavensis   Dmoj\GI21763 Y
Orthologs in non-Drosophila Dipterans (EOG67SSPM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013675   Anopheles gambiae Malaria mosquito  Agam\AGAP011190   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011194
Orthologs in non-Dipteran Insects (EOG67SR9M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB40360   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012739   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH25004   Atta cephalotes Leafcutter ant  Acep\ACEP25972   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18957   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19601   Linepithema humile Argentine ant  Lhum\LH18788   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12579   Solenopsis invicta Red fire ant  Sinv\SINV17331   Acyrthosiphon pisum Pea aphid  Apim\ACYPI009395   Bombyx mori Silkmoth  Bmor\BGIBMGA004302   Pediculus humanus Human body louse  Phum\PHUM551010   Tribolium castaneum Red flour beetle  Tcas\TC010161
Orthologs in non-Insect Arthropods (EOG65MKN4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\ST1A5   Ixodes scapularis Deer tick  Isca\ISCW017360
Orthologs in non-Arthropod Metazoa (EOG6996TR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\Y37E3.10   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003646gn   Danio rerio Zebrafish  Drer\eif2s1l   Danio rerio Zebrafish  Drer\eif2s1   Xenopus tropicalis Western clawed frog  Xtro\eif2s1   Gallus gallus Domestic chicken  Ggal\ENSGALG00000009565   Mus musculus House mouse  Mmus\Eif2s1   Rattus norvegicus Norway rat  Rnor\Eif2s1   Homo sapiens Human  Hsap\EIF2S1
Human Orthologs (1)
Gene OMIM HGNC Hsap\EIF2S1
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15749 Drosophila sechellia Dsec\GM13399 Drosophila erecta Dere\GG19321 Drosophila yakuba Dyak\GE15968 Drosophila ananassae Dana\GF19374 Drosophila pseudoobscura pseudoobscura Dpse\GA22144 Drosophila persimilis Dper\GL20268 Drosophila willistoni Dwil\GK25248 Drosophila virilis Dvir\GJ19141 Drosophila mojavensis Dmoj\GI21763
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	4926 y1 cv1 v1 eIF-2α815-29 f1/C(1)DX/Dp(1;Y)y+; Dp(1;4)r+, fK36/svspa-pol
Kyoto	107951 y1 cv1 v1 eIF-2α815-29 f1/C(1)DX/Dp(1;Y)y+; Df(1;4)r+*, fK36/svspa-pol
VDRC	v104562 P{KK100282}VIE-260B v7799 w1118; P{GD1430}v7799 v7798 w1118; P{GD1430}v7798/CyO
Genomic Clones ( 2 )
	BACR13G13 BACR47D09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 60 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH06180
Other clones	DPiM_GH06180
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT05276 AT05289 AT14946 AT15130 AT25238 GH05906 GH11187 GH16768 GH17367 GM02291 GM02737 GM05225 GM05870 LD09346 LD37833 LP07804 LP09295 LP09486 LP17772 RE12830 RE21805 RE40477 RE53021 RE55583 RE67332 RE67387 RE72125 RE75137 RH18717 RH18789 RH23610 RH34595 RH40562 RH56389 RH63132 RH66016 RH67545 SD11579 SD13374 SD17531 SD22765
Other clones	33889 43647 57220 119328 CK01.64D.F6 CK01.101B.G7 EC14817 EK065255 EK077828 EK085428 EK160907 EK162916 EK177360 EK239137 FGM001C08 FGM220F12 LD21861
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261609 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32617
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: eIF-2α M(1)14C (Marygold et al., 2007)
Additional comments	"l(1)G0272" may affect "eIF-2α". (Peter et al., 2002)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) Minute gene. (Marygold et al., 2007) eIF-2α protein from both control and heat shocked cells has been purified and characterised. (Duncan et al., 1995) eIF-2α has been isolated and characterised. (Qu and Cavener, 1994) eIF-2α nucleotide sequence displays a high degree of similarity with the human and yeast homologs. eIF-2α mRNA displays quasi-constitutive expression. (Ye et al., 1992) eIF-2α mRNA displays quasi-constitutive expression.   One of a class of genes (see MIN record) that when present in one, rather than two, copies, produce a characteristic phenotype consisting of short slender bristles and delayed development.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132902 RefSeq (Proteins) • NP_573130 DDBJ / EMBL / GenBank • AAA53627 AAD38608 AAF48615 AF145633 AI106919 AI402039 L19196 Entrez Gene - A searchable database of RefSeq genes. • 32617 UniProtKB/Swiss-Prot • P41374
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ribosomal protein S1, RNA-binding domain (IPR003029) Translation initiation factor 2, alpha subunit (IPR011488) Nucleic acid-binding, OB-fold (IPR012340) Translation initiation factor 2, alpha subunit, middle domain (IPR024054) Translation initiation factor 2, alpha subunit, C-terminal (IPR024055) RNA-binding domain, S1 (IPR022967)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261609 DRSC - Results from RNAi screens. • FBgn0261609 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004925 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261609 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32617 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32617 modMine - Data generated by the modENCODE project. • FBgn0261609
Synonyms & Secondary IDs ( 26 )
Reported As
Symbol Synonym	CG9946 (Chen et al., 2012, Pile et al., 2003, Lasko, 2000, Goshima et al., 2007, Pisa et al., 2009, Keleman et al., 2009.8.5, Hughes et al., 2008, Kim et al., 2010, Cammarato et al., 2011) deIF2α (Garrey et al., ) eIF-2 (Zapata et al., 1991, Goshima et al., 2007) eIF-2 a (Peter et al., 2002) eIF2a (Lindsay et al., 2008) eIF2-alpha (Lindquist et al., 2011) eIF-2 alpha (McNaughton et al., 2000) eIF-2alpha (Giot et al., 2003, Kopp, 2009.5.20, Cook et al., 2012) eIF2 alpha (Ryoo and Steller, 2007) eIF-2α (Matta et al., 2011, Marygold et al., 2007, Stuart et al., 2007, Pisa et al., 2009, Lindquist et al., 2011) eIF2α (Mounir et al., 2011, Pomar et al., 2003, Williams et al., 2001, Lasko, 2000, Ryoo and Steller, 2007, Haecker et al., 2008, Farny et al., 2009, Carra et al., 2010, Kim et al., 2012, Chakrabarti et al., 2012) Eif2α   eIF2-α (Andersen and Leevers, 2007) eiF-2α (Kim et al., 2010) elF2α (Ye et al., 1992) IF2A_DROME (Storm and Sonnhammer, 2003) l(1)14Cf (Banga, 1996.5.2, Banga et al., 1995) l(1)19-153 (Banga, 1996.5.2, Banga et al., 1995) M(1)8e3-10 (Stanewsky et al., 1993, ) M(1)14C (Marygold et al., 2007, ) M(1)19-153
Name Synonym	eIF-2alpha (Cammarato et al., 2011) eIF-2α   Eukaryotic initiation factor 2α   eukaryotic translation Initiation Factor 2 (Mounir et al., 2011) eukaryotic translation initiation factor 2α (Pisa et al., 2009) eukaryotic translation Initiation Factor 2α   Minute (1) 14C
Secondary FlyBase IDs
	FBgn0002589 FBgn0004925 FBgn0024971
References ( 63 )
Generate a list of	All references relating to this gene ( 63 )
List References by type	Research paper  ( 38 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 3 ) FlyBase analysis  ( 2 ) Protein sequence record  ( 1 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 10 )
	Chakrabarti et al., 2012, Cell Host Microbe 12(1): 60--70 Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. [FBrf0218984] Chen et al., 2012, Cell Death Differ. 19(10): 1655--1663 Effectors of alcohol-induced cell killing in Drosophila. [FBrf0219410] Cook et al., 2012, Genome Biol. 13(3): R21 The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome. [FBrf0219066] Kim et al., 2012, J. Biol. Chem. 287(9): 6628--6641 Glutathione s-transferase omega 1 activity is sufficient to suppress neurodegeneration in a Drosophila model of Parkinson disease. [FBrf0217543] Krauchunas et al., 2012, Dev. Biol. 370(1): 125--134 Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster. [FBrf0219342] Penney et al., 2012, Neuron 74(1): 166--178 TOR Is Required for the Retrograde Regulation of Synaptic Homeostasis at the Drosophila Neuromuscular Junction. [FBrf0218076] Casad et al., 2011, Genetics 189(3): 861--870 Cardiomyopathy Is Associated with Ribosomal Protein Gene Haplo-Insufficiency in Drosophila melanogaster. [FBrf0216591] Lindquist et al., 2011, Genome Res. 21(3): 433--446 Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Matta et al., 2011, Dev. Genes Evol. 221(1): 49--57 Getting real with real-time qPCR: a case study of reference gene selection for morphological variation in Drosophila melanogaster wings. [FBrf0213646] Mounir et al., 2011, Sci. Signal. 4(192): ra62 Akt Determines Cell Fate Through Inhibition of the PERK-eIF2{alpha} Phosphorylation Pathway. [FBrf0216247] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...