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Gene Dmel\nej

General Information
Symbol	Dmel\nej	Species	D. melanogaster
Name	nejire	Annotation symbol	CG15319
Feature type	protein_coding_gene	FlyBase ID	FBgn0261617
Gene Model Status	Current	Stock availability	27 publicly available
Also Known As	dCBP, CBP, CG15321, CBP/p300, p300/CBP
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	8F7-8F9	Sequence location	X:9,559,693..9,581,099 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene nejire is referred to in FlyBase by the symbol Dmel\nej (CG15319, FBgn0261617). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: histone acetyltransferase activity (H3-K27 specific); protein binding; transcription factor binding; histone acetyltransferase activity; histone acetyltransferase activity (H3-K18 specific); transcription coactivator activity. There is experimental evidence for 17 unique biological process terms, many of which group under: biological regulation; cellular component organization or biogenesis; sensory organ development; macromolecule modification; organelle organization; system development; rhythmic process; compound eye morphogenesis; neuron differentiation; chromosome organization. 60 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; external compound sense organ; adult segment; cephalopharyngeal skeleton; imaginal precursor; peripheral nervous system; eo support cell; acellular anatomical structure; larval abdominal segment. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Bromodomain; Bromodomain, conserved site; Coactivator CBP, KIX; Domain of unknown function DUF902, CREBbp; Histone H3-K56 acetyltransferase, RTT109; Nuclear receptor coactivator, CREB-bp-like, interlocking; Nuclear receptor coactivator, interlocking; Zinc finger, PHD-type, conserved site; Zinc finger, TAZ-type; Zinc finger, ZZ-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, larval trachea, adult ovary, larval carcass. Gene sequence location is X:9559693..9581099.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	References Schmidt et al., 2011, Glia 59(9): 1264--1272 Sela et al., 2010, Genome Biol. 11(6): R59 Herz et al., 2010, Mol. Cell. Biol. 30(10): 2485--2497 Guenin et al., 2010, BMC Genomics 11: 47 Kirilly et al., 2011, Neuron 72(1): 86--100 Sequence features BKN21411 HFA18801 HFA18006 BKN33191 Controlled Vocabulary Terms embryonic stage 5 embryonic stage 12 embryonic stage 11 organism embryonic stage 8 gastrula stage embryonic stage 6 embryonic stage 9 early extended germ band stage ubiquitous embryonic stage 4 embryonic stage 10 embryonic stage 7 Clones 79695 EK194802 32961 146843 EK249549 104496 EK142817 214565.12 EP11651 CK00533 CK01.39A.H4 GH08927 160072 EK229233
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 8F7-8F9   Limits computationally determined from genome sequence between P{EP}EP912EP912 and P{EP}nejEP1149&P{EP}nejEP1179
Experimentally Determined Cytological Location
Cytogenetic map Notes References 8F4-8F8   (determined by in situ hybridisation)   (Peter et al., 2002) 8F4-8F7   (determined by in situ hybridisation)   (Schaefer et al., 1999.12.2) 8F-9A     (Chen, 1997.2.5) 8F6-8F7   (determined by in situ hybridisation)   (Schaefer et al., 1999.8.12) 8F4-8F7   (determined by in situ hybridisation)   (Schaefer et al., 1999.6.15) 8F-9A   (determined by in situ hybridisation)   (Akimaru et al., 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nej for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42 Gene model reviewed during 5.43 DGC clone LD33277 appears problematic: incomplete CDS The 5' non-translated part of nej was previously annotated as a separate gene, CG15321, which has been withdrawn.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nej-RB FBtr0071402  NM_079903   11733   3276 nej-RC FBtr0302722  NM_001201646   12001   3282 nej-RD FBtr0302723  NM_001201647   15511   3276
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nej-PB   FBpp0071337   340.7   3276   8.86     NP_524642   AAF46516 nej-PC   FBpp0291862   341.4   3282   8.89     NP_001188575   ADV37657 nej-PD   FBpp0291863   340.7   3276   8.86     NP_001188576   ADV37658
Additional Polypeptide Data & Comments
Reported size (kDa)	3190 (aa); 332 (kD) (Akimaru et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Bromodomain, conserved site (IPR018359) Zinc finger, TAZ-type (IPR000197) Zinc finger, ZZ-type (IPR000433) Bromodomain (IPR001487) Coactivator CBP, KIX (IPR003101) Domain of unknown function DUF902, CREBbp (IPR010303) Nuclear receptor coactivator, interlocking (IPR009110) Zinc finger, PHD-type, conserved site (IPR019786) Nuclear receptor coactivator, CREB-bp-like, interlocking (IPR014744) Histone H3-K56 acetyltransferase, RTT109 (IPR013178)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140885   AA140886   AA246425   AA246458   AA439032   AA439695   AA979031   AE014298 AAF46516   AE014298 ADV37657   AE014298 ADV37658   AI109676   AJ426814   AJ426817   AJ426819   AQ025365   AQ025395   AQ025399   AQ025447   AW943174   AX189291   AX777235   AY058635 AAL13864   AY089341   BF491006   BI353161   BI481721   BI482473   BI621706   BI626220   BI627283   BI634921   BP548778   BT030955   BT030977   CK662210   CO152767   CO155715   CO156642   CO265498   CO270481   CO289723   CO305853   CO312543   CO321749   CO324761   CO340976   DQ327754   EC057662   EC064212   EC200365   EC202464   EC208398   EC209739   EC215249   EC231415   EC237946   EC242210   EC244696   EC250157   EC256741   EC265811   EC266546   EC267521   ES692130   ES694227   EV594535   U88570 AAB53050
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O01368 Q95TP4 Q9W321 Q9W320
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 12   organism | ubiquitous   (Ludlam et al., 2002) embryonic stage 5     • nucleus (Lilja et al., 2003)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0261617    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0261617 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0261617 expression level Developmental Stage   Expression Level embryo 00-02hr    6858 embryo 02-04hr    7851 embryo 04-06hr    4100 embryo 06-08hr    4363 embryo 08-10hr    3121 embryo 10-12hr    1195 embryo 12-14hr    1860 embryo 14-16hr    3785 embryo 16-18hr    2613 embryo 18-20hr    1920 embryo 20-22hr    4838 embryo 22-24hr    2860 larva L1    1114 larva L2    707 larva L3 12hr old    899 larva L3 puffstage 1-2    474 larva L3 puffstage 3-6    1431 larva L3 puffstage 7-9    1113 white prepupae new    1105 white prepupae 12hr    1313 white prepupae 24hr    1619 pupae 2d postWPP    959 pupae 3d postWPP    573 pupae 4d postWPP    771 adult male 01day    787 adult male 05day    1099 adult male 30day    1176 adult female 01day    2679 adult female 05day    3437 adult female 30day    3741 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (6858) embryo 02-04hr  (7851) embryo 04-06hr  (4100) embryo 06-08hr  (4363) embryo 08-10hr  (3121) embryo 10-12hr  (1195) embryo 12-14hr  (1860) embryo 14-16hr  (3785) embryo 16-18hr  (2613) embryo 18-20hr  (1920) embryo 20-22hr  (4838) embryo 22-24hr  (2860) larva L1  (1114) larva L2  (707) larva L3 12hr old  (899) larva L3 puffstage 1-2  (474) larva L3 puffstage 3-6  (1431) larva L3 puffstage 7-9  (1113) white prepupae new  (1105) white prepupae 12hr  (1313) white prepupae 24hr  (1619) pupae 2d postWPP  (959) pupae 3d postWPP  (573) pupae 4d postWPP  (771) adult male 01day  (787) adult male 05day  (1099) adult male 30day  (1176) adult female 01day  (2679) adult female 05day  (3437) adult female 30day  (3741) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (6858) embryo 02-04hr  (7851) embryo 04-06hr  (4100) embryo 06-08hr  (4363) embryo 08-10hr  (3121) embryo 10-12hr    1195 embryo 12-14hr  (1860) embryo 14-16hr  (3785) embryo 16-18hr  (2613) embryo 18-20hr  (1920) embryo 20-22hr  (4838) embryo 22-24hr  (2860) larva L1    1114 larva L2    707 larva L3 12hr old    899 larva L3 puffstage 1-2    474 larva L3 puffstage 3-6  1431 larva L3 puffstage 7-9    1113 white prepupae new    1105 white prepupae 12hr    1313 white prepupae 24hr  (1619) pupae 2d postWPP    959 pupae 3d postWPP    573 pupae 4d postWPP    771 adult male 01day    787 adult male 05day    1099 adult male 30day    1176 adult female 01day  (2679) adult female 05day  (3437) adult female 30day  (3741) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6858 embryo 02-04hr    7851 embryo 04-06hr    4100 embryo 06-08hr    4363 embryo 08-10hr    3121 embryo 10-12hr    1195 embryo 12-14hr    1860 embryo 14-16hr    3785 embryo 16-18hr    2613 embryo 18-20hr    1920 embryo 20-22hr    4838 embryo 22-24hr    2860 larva L1    1114 larva L2    707 larva L3 12hr old    899 larva L3 puffstage 1-2    474 larva L3 puffstage 3-6    1431 larva L3 puffstage 7-9    1113 white prepupae new    1105 white prepupae 12hr    1313 white prepupae 24hr    1619 pupae 2d postWPP    959 pupae 3d postWPP    573 pupae 4d postWPP    771 adult male 01day    787 adult male 05day    1099 adult male 30day    1176 adult female 01day    2679 adult female 05day    3437 adult female 30day    3741 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6858 embryo 02-04hr    7851 embryo 04-06hr    4100 embryo 06-08hr    4363 embryo 08-10hr    3121 embryo 10-12hr    1195 embryo 12-14hr    1860 embryo 14-16hr    3785 embryo 16-18hr    2613 embryo 18-20hr    1920 embryo 20-22hr    4838 embryo 22-24hr    2860 larva L1    1114 larva L2    707 larva L3 12hr old    899 larva L3 puffstage 1-2    474 larva L3 puffstage 3-6    1431 larva L3 puffstage 7-9    1113 white prepupae new    1105 white prepupae 12hr    1313 white prepupae 24hr    1619 pupae 2d postWPP    959 pupae 3d postWPP    573 pupae 4d postWPP    771 adult male 01day    787 adult male 05day    1099 adult male 30day    1176 adult female 01day    2679 adult female 05day    3437 adult female 30day    3741 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0261617 expression level Developmental Stage   Expression Level embryo 00-02hr    6858 embryo 02-04hr    7851 embryo 04-06hr    4100 embryo 06-08hr    4363 embryo 08-10hr    3121 embryo 10-12hr    1195 embryo 12-14hr    1860 embryo 14-16hr    3785 embryo 16-18hr    2613 embryo 18-20hr    1920 embryo 20-22hr    4838 embryo 22-24hr    2860 larva L1    1114 larva L2    707 larva L3 12hr old    899 larva L3 puffstage 1-2    474 larva L3 puffstage 3-6    1431 larva L3 puffstage 7-9    1113 white prepupae new    1105 white prepupae 12hr    1313 white prepupae 24hr    1619 pupae 2d postWPP    959 pupae 3d postWPP    573 pupae 4d postWPP    771 adult male 01day    787 adult male 05day    1099 adult male 30day    1176 adult female 01day    2679 adult female 05day    3437 adult female 30day    3741 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (6858) embryo 02-04hr  (7851) embryo 04-06hr  (4100) embryo 06-08hr  (4363) embryo 08-10hr  (3121) embryo 10-12hr  (1195) embryo 12-14hr  (1860) embryo 14-16hr  (3785) embryo 16-18hr  (2613) embryo 18-20hr  (1920) embryo 20-22hr  (4838) embryo 22-24hr  (2860) larva L1  (1114) larva L2  (707) larva L3 12hr old  (899) larva L3 puffstage 1-2  (474) larva L3 puffstage 3-6  (1431) larva L3 puffstage 7-9  (1113) white prepupae new  (1105) white prepupae 12hr  (1313) white prepupae 24hr  (1619) pupae 2d postWPP  (959) pupae 3d postWPP  (573) pupae 4d postWPP  (771) adult male 01day  (787) adult male 05day  (1099) adult male 30day  (1176) adult female 01day  (2679) adult female 05day  (3437) adult female 30day  (3741) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (6858) embryo 02-04hr  (7851) embryo 04-06hr  (4100) embryo 06-08hr  (4363) embryo 08-10hr  (3121) embryo 10-12hr    1195 embryo 12-14hr  1860 embryo 14-16hr  (3785) embryo 16-18hr  (2613) embryo 18-20hr  1920 embryo 20-22hr  (4838) embryo 22-24hr  (2860) larva L1    1114 larva L2    707 larva L3 12hr old    899 larva L3 puffstage 1-2    474 larva L3 puffstage 3-6  1431 larva L3 puffstage 7-9    1113 white prepupae new    1105 white prepupae 12hr    1313 white prepupae 24hr  1619 pupae 2d postWPP    959 pupae 3d postWPP    573 pupae 4d postWPP    771 adult male 01day    787 adult male 05day    1099 adult male 30day    1176 adult female 01day  (2679) adult female 05day  (3437) adult female 30day  (3741) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6858 embryo 02-04hr    7851 embryo 04-06hr    4100 embryo 06-08hr    4363 embryo 08-10hr    3121 embryo 10-12hr    1195 embryo 12-14hr    1860 embryo 14-16hr    3785 embryo 16-18hr    2613 embryo 18-20hr    1920 embryo 20-22hr    4838 embryo 22-24hr    2860 larva L1    1114 larva L2    707 larva L3 12hr old    899 larva L3 puffstage 1-2    474 larva L3 puffstage 3-6    1431 larva L3 puffstage 7-9    1113 white prepupae new    1105 white prepupae 12hr    1313 white prepupae 24hr    1619 pupae 2d postWPP    959 pupae 3d postWPP    573 pupae 4d postWPP    771 adult male 01day    787 adult male 05day    1099 adult male 30day    1176 adult female 01day    2679 adult female 05day    3437 adult female 30day    3741 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6858 embryo 02-04hr    7851 embryo 04-06hr    4100 embryo 06-08hr    4363 embryo 08-10hr    3121 embryo 10-12hr    1195 embryo 12-14hr    1860 embryo 14-16hr    3785 embryo 16-18hr    2613 embryo 18-20hr    1920 embryo 20-22hr    4838 embryo 22-24hr    2860 larva L1    1114 larva L2    707 larva L3 12hr old    899 larva L3 puffstage 1-2    474 larva L3 puffstage 3-6    1431 larva L3 puffstage 7-9    1113 white prepupae new    1105 white prepupae 12hr    1313 white prepupae 24hr    1619 pupae 2d postWPP    959 pupae 3d postWPP    573 pupae 4d postWPP    771 adult male 01day    787 adult male 05day    1099 adult male 30day    1176 adult female 01day    2679 adult female 05day    3437 adult female 30day    3741 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0261617    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0261617 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, larval trachea, adult ovary, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015624
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal nej3 nejG0112 nejG0350 nejG0470 lethal, with Scer\GAL41407 nejGD8975 lethal, with Scer\GAL4pnr-MD237 nejGD1494 lethal (with nej3) nejP lethal (with nejP) nej3 nejQ7 nejS103 nejS342 nejTA57 lethal (with nejQ7) nejP lethal (with nejS103) nejP lethal (with nejS342) nejP lethal (with nejTA57) nejP lethal | adult stage nejG0112 lethal | embryonic stage | recessive nej3 nejQ7 lethal | larval stage, with Scer\GAL4elav.PLu nejEP1149 lethal | pharate adult stage nejG0112 lethal | pharate adult stage, with Scer\GAL430A nejScer\UAS.cMa lethal | prepupal stage nejG0470 lethal | prepupal stage, with Scer\GAL4GMR.PF nejΔQ.Scer\UAS lethal | P-stage nejG0112 nejG0350 nejG0470 lethal | pupal stage, with Scer\GAL4Act5C.PI nejdsRNA.Scer\UAS.cKa lethal | pupal stage, with Scer\GAL4GMR.PF nejΔQ.Scer\UAS lethal | pupal stage, with Scer\GAL4pnr-MD237 nejGD8975 lethal | recessive nej3 nejG0350 nejS103 nejS342 nejTA57 nejWA69 semi-lethal | embryonic stage, with Scer\GAL4Act5C.PI nejdsRNA.Scer\UAS.cKa semi-lethal | larval stage, with Scer\GAL4Act5C.PI nejdsRNA.Scer\UAS.cKa semi-lethal | pharate adult stage, with Scer\GAL430A nejScer\UAS.cMa semi-lethal | pharate adult stage, with Scer\GAL4OK363 nejScer\UAS.cMa semi-lethal | recessive nejG0112 nejG0470 semi-viable nejS342 nejTA57 viable, with Scer\GAL4[-]: nejEP1149 nejEP1179 nejScer\UAS.cMa Sterility Allele male sterile nejG0350 Other Phenotypes Allele developmental rate defective | recessive nejS342 nejTA57 developmental rate defective | recessive, with Scer\GAL4[-]: nejEP1149 nejEP1179 eye color defective, with Scer\GAL4GMR.PF nejdsRNA.Scer\UAS.cKa hypoactive | recessive nejS342 nejTA57 hypoactive | recessive, with Scer\GAL4[-]: nejEP1149 nejEP1179 mitotic cell cycle defective | third instar larval stage, with Scer\GAL4hs.PB nejdsRNA.Scer\UAS neuroanatomy defective nejQ7 nejS342 nejTA57 neuroanatomy defective, with Scer\GAL4elav.PLu nejEP1149 nejEP1179 neuroanatomy defective | larval stage, with Scer\GAL41407 nejGD8975 neurophysiology defective nejQ7 nejS342 nejTA57 neurophysiology defective, with Scer\GAL4elav.PLu nejEP1149 nejEP1179 nejScer\UAS.cMa neurophysiology defective, with Scer\GAL4Mhc.PW nejScer\UAS.cMa visible, with Scer\GAL430A nejScer\UAS.cMa visible, with Scer\GAL4bbg-C96 nejScer\UAS.T:SV5\V5 visible, with Scer\GAL4DBD.GMR nejScer\UAS.cMa visible, with Scer\GAL4ey.PH nejdsRNA.Scer\UAS.cKa nejΔNZK.Scer\UAS nejΔQ.Scer\UAS visible, with Scer\GAL4GMR.PF nejdsRNA.Scer\UAS.cKa nejEP1149 nejEP1179 nejF2161A.Scer\UAS.T:SV5\V5 nejScer\UAS.T:SV5\V5 nejY2160A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔNZK.Scer\UAS visible, with Scer\GAL4GMR.PF, nej3 nejΔBHQ.Scer\UAS nejΔNZK.Scer\UAS visible, with Scer\GAL4GMR.PF, nejΔBHQ.Scer\UAS nej3 visible, with Scer\GAL4GMR.PF, nejΔNZK.Scer\UAS nej3 visible, with Scer\GAL4hs.2sev nejΔBHQ.Scer\UAS nejΔQ.Scer\UAS visible, with Scer\GAL4hs.PB nejdsRNA.Scer\UAS.cKa visible, with Scer\GAL4OK363 nejScer\UAS.cMa visible, with Scer\GAL4OK386 nejScer\UAS.cMa visible, with Scer\GAL4sca-537.4 nejEP1149 visible (with nej131) nejP visible (with nejP) nej131 nejTC41 visible (with nejTC41) nejP visible | recessive | somatic clone nejTC41 wild-type nejhs.PA Phenotype manifest in Allele abdominal 4 ventral denticle belt nej1 abdominal 5 ventral denticle belt nej1 adult abdominal segment 4 | ectopic, with Scer\GAL4hs.PB nejdsRNA.Scer\UAS.cKa adult abdominal segment 5, with Scer\GAL4hs.PB nejdsRNA.Scer\UAS.cKa amnioserosa | germline clone nej1 cephalopharyngeal skeleton nej3 condensed chromosome | third instar larval stage, with Scer\GAL4hs.PB nejdsRNA.Scer\UAS cone cell | ectopic, with Scer\GAL4GMR.PF nejScer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS cuticle nej3 denticle belt | anterior nej3 dorsocentral bristle, with Scer\GAL4sca-537.4 nejEP1149 ectoderm | dorsal | germline clone nej1 embryonic/first instar larval cuticle nej1 embryonic/larval dorsal trunk nej3 embryonic/larval midgut | embryonic stage nej3 embryonic/larval midgut | embryonic stage | germline clone nej1 embryonic/larval proventriculus | embryonic stage nejQ7 nejTA57 embryonic/larval salivary gland nej3 nejG0350 nejG0112 nejG0470 embryonic/larval salivary gland, with Scer\GAL4SG nejdsRNA.Scer\UAS.cYa embryonic/larval tracheal system nej3 embryonic/larval ventral anastomosis nej3 embryonic antennal sense organ nejQ7 nejTA57 embryonic head nej1 epidermis nej1 nej2 eye, with Scer\GAL4DBD.GMR nejScer\UAS.cMa eye, with Scer\GAL4ey.PH nejdsRNA.Scer\UAS.cKa nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS nejΔQ.Scer\UAS eye, with Scer\GAL4GMR.PF nejScer\UAS.T:SV5\V5 nejdsRNA.Scer\UAS.cKa nejY2160A.Scer\UAS.T:SV5\V5 nejF2161A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS nejΔNZK.Scer\UAS nejEP1149 nejEP1179 eye, with Scer\GAL4GMR.PF, nej3 nejΔBHQ.Scer\UAS nejΔNZK.Scer\UAS eye, with Scer\GAL4GMR.PF, nejΔBHQ.Scer\UAS nej3 eye, with Scer\GAL4GMR.PF, nejΔNZK.Scer\UAS nej3 eye, with Scer\GAL4hs.2sev nejΔBHQ.Scer\UAS nejΔQ.Scer\UAS eye (with nej131) nejP eye (with nejP) nejTC41 nej131 eye (with nejTC41) nejP eye | dorsal, with Scer\GAL4ey.PH nejΔNZK.Scer\UAS eye | somatic clone nejTC41 eye disc, with Scer\GAL4ey.PH nejΔQ.Scer\UAS eye disc, with Scer\GAL4GMR.PF nejΔBHQ.Scer\UAS nejΔNZK.Scer\UAS eye disc | third instar larval stage, with Scer\GAL4hs.PB nejdsRNA.Scer\UAS eye photoreceptor cell, with Scer\GAL4GMR.PF nejScer\UAS.T:SV5\V5 nejdsRNA.Scer\UAS.cKa nejF2161A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS nejΔNZK.Scer\UAS eye photoreceptor cell, with Scer\GAL4hs.2sev nejΔQ.Scer\UAS eye photoreceptor cell (with nej131) nejP eye photoreceptor cell (with nejP) nej131 eye photoreceptor cell | ectopic, with Scer\GAL4GMR.PF nejΔNZK.Scer\UAS eye photoreceptor cell | somatic clone nejTC41 filzkorper nej3 ganglion mother cell | larval stage, with Scer\GAL41407 nejGD8975 gastric caecum nej3 head nej3 nej1 nej2 interommatidial bristle, with Scer\GAL4GMR.PF nejScer\UAS.T:SV5\V5 nejdsRNA.Scer\UAS.cKa nejY2160A.Scer\UAS.T:SV5\V5 nejF2161A.Scer\UAS.T:SV5\V5 nejΔNZK.Scer\UAS interommatidial bristle, with Scer\GAL4hs.2sev nejΔBHQ.Scer\UAS macrochaeta nej3 median tooth nejQ7 nejTA57 midgut constriction 1 nej3 mouth hooks nejQ7 nejTA57 mystery cell, with Scer\GAL4GMR.PF nejΔNZK.Scer\UAS neuromuscular junction nejQ7 nejS342 nejTA57 neuromuscular junction, with Scer\GAL4[-]: nejEP1149 nejEP1179 neuromuscular junction, with Scer\GAL4elav.PLu nejScer\UAS.cMa nejEP1149 nejEP1179 neuromuscular junction, with Scer\GAL4Mhc.PW nejScer\UAS.cMa neuromuscular junction, with Scer\GAL4Mhc.PW, Scer\GAL4elav.PLu nejEP1179 neuromuscular junction & bouton nejQ7 nejS342 nejTA57 neuromuscular junction & bouton, with Scer\GAL4[-]: nejEP1149 nejEP1179 neuromuscular junction & bouton, with Scer\GAL4elav.PLu nejEP1179 neuromuscular junction & t-bar, with Scer\GAL4[-]: nejEP1149 ommatidial precursor cluster, with Scer\GAL4GMR.PF nejScer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS nejΔNZK.Scer\UAS nejΔQ.Scer\UAS ommatidium, with Scer\GAL4ey.PH nejdsRNA.Scer\UAS.cKa ommatidium, with Scer\GAL4GMR.PF nejScer\UAS.T:SV5\V5 nejdsRNA.Scer\UAS.cKa nejY2160A.Scer\UAS.T:SV5\V5 nejF2161A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS nejΔNZK.Scer\UAS ommatidium, with Scer\GAL4hs.2sev nejF2161A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS nejΔQ.Scer\UAS ommatidium (with nej131) nejP ommatidium (with nejP) nejTC41 nej131 ommatidium (with nejTC41) nejP ommatidium | somatic clone nejTC41 outer photoreceptor cell | ectopic, with Scer\GAL4hs.2sev nejKIX.Scer\UAS photoreceptor cell R3, with Scer\GAL4hs.2sev nejF2161A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS photoreceptor cell R3 | ectopic, with Scer\GAL4hs.2sev nejF2161A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS photoreceptor cell R4, with Scer\GAL4hs.2sev nejF2161A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS photoreceptor cell R7, with Scer\GAL4hs.2sev nejKIX.Scer\UAS nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS photoreceptor cell R7 | ectopic, with Scer\GAL4hs.2sev nejF2161A.Scer\UAS.T:SV5\V5 nejΔBHQ.Scer\UAS nejΔHQ.Scer\UAS pigment cell | somatic clone nejTC41 retina, with Scer\GAL4GMR.PF nejScer\UAS.T:SV5\V5 rhabdomere, with Scer\GAL4GMR.PF nejdsRNA.Scer\UAS.cKa nejF2161A.Scer\UAS.T:SV5\V5 scutellar bristle, with Scer\GAL4sca-537.4 nejEP1149 terminal organ | embryonic stage nejQ7 nejTA57 thorax nej3 nej1 nej2 tormogen cell, with Scer\GAL4sca-537.4 nejEP1149 trichogen cell, with Scer\GAL4sca-537.4 nejEP1149 wing, with Scer\GAL430A nejScer\UAS.cMa wing, with Scer\GAL4bbg-C96 nejScer\UAS.T:SV5\V5 wing, with Scer\GAL4OK363 nejScer\UAS.cMa wing, with Scer\GAL4OK386 nejScer\UAS.cMa
Classical Alleles ( 26 )
For All Classical Alleles Show
Allele of nej Class Mutagen Stocks Known lesion nej3 P-element activity 2 Yes nejG0119a 2 -- nejQ7 antimorphic allele - genetic evidence ethyl methanesulfonate 2 -- nej0.3 ethyl methanesulfonate 1 -- nejEP1179 hypomorphic allele - genetic evidence P-element activity 1 Yes nejEP950 P-element activity 1 Yes nejG0112 P-element activity 1 -- nejG0350 P-element activity 1 -- nejG0470 P-element activity 1 -- nejP P-element activity 1 Yes nej131 0 -- nej1 0 -- nej2 0 -- nejCPTI000727 piggyBac transposase 0 Yes nejEP1149 hypomorphic allele - genetic evidence P-element activity 0 Yes nejEP1410 P-element activity 0 Yes nejEP2246 0 -- nejGG01399 0 -- nejGS1188 0 -- nejKG06619 0 -- nejS103 ethyl methanesulfonate 0 -- nejS342 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- nejTA57 ethyl methanesulfonate 0 -- nejTC41 hypomorphic allele - genetic evidence 0 -- nejunspecified 0 -- nejWA69 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 34 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nej Class Mutagen Stocks Known lesion nejdsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 3 Yes nejF2161A.Scer\UAS.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - coding region fusion 2 Yes nejGD8975 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes nej+t16 in vitro construct - genomic fragment 1 Yes nejGD1494 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nejHM04037 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nejhs.PA in vitro construct - regulatory fusion 1 Yes nejJF02806 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nejKK101360 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nejKK113191 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nejScer\UAS.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes nej1677-2608.Act5C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nej2040-3190.Act5C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nej383-1090.Act5C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nejAct5C.PA in vitro construct - regulatory fusion 0 -- nejCMV.cFa in vitro construct - regulatory fusion 0 Yes nejdsRNA.cFa in vitro construct - RNAi 0 Yes nejdsRNA.cRa in vitro construct - RNAi 0 Yes nejdsRNA.Scer\UAS.cKa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes nejdsRNA.Scer\UAS.cYa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes nejF2161A.MtnA.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nejHATmut.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes nejHMS01507 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes nejKIX.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes nejKr.PL FLPase 0 Yes nejKr.Scer\FRT in vitro construct - regulatory fusion in vitro construct 0 Yes nejMtnA.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nejScer\UAS.cMa in vitro construct - regulatory fusion 0 Yes nejScer\UAS.cYa in vitro construct - regulatory fusion 0 Yes nejY2160A.Scer\UAS.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes nejΔBHQ.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes nejΔHQ.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes nejΔNZK.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes nejΔQ.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Not duplicated in	Dp(1;2)v+75d (Florence and McGinnis, 1998) Dp(1;3)wm49a (Florence and McGinnis, 1998)
Not disrupted in	Df(1)9a4-5 (Florence and McGinnis, 1998) Df(1)dm75e19 (Florence and McGinnis, 1998) Df(1)lz-90b24 (Florence and McGinnis, 1998) Df(1)v-L15 (Florence and McGinnis, 1998)
Duplicated in	Dp(1;3)DC212 (Popodi et al., 2010-) Dp(1;Y)FF1 (Florence and McGinnis, 1998) Dp(1;Y)lz+ (Florence and McGinnis, 1998)
Disrupted in	Df(1)C52 (Florence and McGinnis, 1998)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1494} NA P{KK101360} NA P{TRiP.HM04037} NA P{TRiP.HMS01507} NA P{TRiP.JF02806} NA P{UAS-nej.F2161A-V5} NA P{UAS-nej.KIX} NA P{UAS-nej.M} NA P{UAS-nej.RNAi.Y} NA P{UAS-nej.RNAi} NA P{UAS-nej.siRNA} NA P{UAS-nej.wt-V5} NA P{UAS-nej.Y2160A-V5} NA P{UAS-nej.Y} NA P{UAS-nej.ΔBHQ} NA P{UAS-nej.ΔHQ} NA P{UAS-nej.ΔNZK} NA P{UAS-nej.ΔQ} NA heat-shock construct P{hs-nej+} NA characterization construct P{Kr(FRT.stop)nej} NA P{Kr(-FRT)nej} NA P{nej+t16} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}nejEP950 NA P{EP}nejEP1149 NA P{EP}nejEP1179 NA P{EP}nejEP1410 NA P{EP}nejEP2246 NA P{GSV1}btdGS1188 NA P{Mae-UAS.6.11}B0457B0457 NA P{Mae-UAS.6.11}B2010B2010 NA P{Mae-UAS.6.11}B2011B2011 NA P{Mae-UAS.6.11}B2012B2012 NA P{Mae-UAS.6.11}GG01052GG01052 NA P{Mae-UAS.6.11}nejGG01399 NA miscellaneous insertions P{GT1}BG01222 NA P{GT1}BG02133 NA P{SUPor-P}KG07347 NA P{SUPor-P}nejKG06619 NA PBac{602.P.SVS-1}nejCPTI000727 NA insertion of enhancer trap P{lacW}l(1)G0137G0137 No P{lacW}l(1)G0236G0236 No P{lacW}l(1)G0270G0270 No P{lacW}l(1)G0286G0286 No P{lacW}l(1)G0400G0400 No P{lacW}l(1)G0464G0464 No P{lacW}nejG0112 No P{lacW}nejG0119a No P{lacW}nejG0350 No P{lacW}nejG0470 No P{lacW}nejP No
Gene Ontology: Function, Process & Cellular Component ( 30 unique terms )
Terms Based on Experimental Evidence ( 25 terms )
Molecular Function
CV term Evidence References
histone acetyltransferase activity inferred from direct assay (Ludlam et al., 2002) histone acetyltransferase activity (H3-K18 specific) inferred from direct assay (Tie et al., 2009) histone acetyltransferase activity (H3-K27 specific) inferred from direct assay (Tie et al., 2009) protein binding inferred from physical interaction with Clk (Hung et al., 2007) inferred from physical interaction with mod (Bantignies et al., 2002) transcription coactivator activity inferred from direct assay (Hung et al., 2007) inferred from genetic interaction with ci (Chen et al., 2000) inferred from genetic interaction with per (Hung et al., 2007) transcription factor binding inferred from physical interaction with ci (Chen et al., 2000)
Biological Process
CV term Evidence References
circadian regulation of gene expression inferred from mutant phenotype (Hung et al., 2007) compound eye development inferred from mutant phenotype (Mallik and Lakhotia, 2010) compound eye morphogenesis inferred from mutant phenotype (Kumar et al., 2004) DNA replication checkpoint inferred from mutant phenotype (Smolik and Jones, 2007) histone H3-K18 acetylation inferred from direct assay (Tie et al., 2009) histone H3-K27 acetylation inferred from direct assay (Tie et al., 2009) histone H4-K8 acetylation inferred from mutant phenotype (Ludlam et al., 2002) histone H4-K12 acetylation inferred from mutant phenotype (Ludlam et al., 2002) locomotor rhythm inferred from mutant phenotype (Hung et al., 2007) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) neurotransmitter secretion inferred from mutant phenotype (Marek et al., 2000) positive regulation of transcription from RNA polymerase II promoter inferred from genetic interaction with ci (Chen et al., 2000) inferred from mutant phenotype (Chen et al., 2000) R3/R4 cell fate commitment inferred from mutant phenotype (Kumar et al., 2004) R7 cell differentiation inferred from mutant phenotype (Kumar et al., 2004) regulation of mitosis inferred from genetic interaction with Myb AND inferred from mutant phenotype (Fung et al., 2003) smoothened signaling pathway inferred from mutant phenotype (Chen et al., 2000) synapse assembly inferred from mutant phenotype (Marek et al., 2000)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Marek et al., 2000, Bantignies et al., 2002) protein complex inferred from physical interaction with mod (Bantignies et al., 2002)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
cAMP response element binding protein binding traceable author statement (Marek et al., 2000) transcription coactivator activity inferred from sequence or structural similarity (Chen et al., 1996) inferred from sequence or structural similarity with mouse Crebbp (FlyBase, 1992-) zinc ion binding inferred from electronic annotation with InterPro:IPR000197, InterPro:IPR000433 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
hemopoiesis traceable author statement (Evans et al., 2003) smoothened signaling pathway non-traceable author statement (Fry and Peterson, 2001) Wnt receptor signaling pathway non-traceable author statement (Fry and Peterson, 2001)
Cellular Component
CV term Evidence References
histone acetyltransferase complex inferred from electronic annotation with InterPro:IPR014744 (FlyBase Curators et al., 2004-) nucleus inferred from sequence or structural similarity (Chen et al., 1996)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nej allele Gene References nej3 arm (Waltzer and Bienz, 1998) ci (Akimaru et al., 1997, Florence and McGinnis, 1998) dpp (Waltzer and Bienz, 1999) Myb (Fung et al., 2003) sax (Waltzer and Bienz, 1999) sgg (Waltzer and Bienz, 1998) shg (Waltzer and Bienz, 1998) so (Kumar et al., 2004) Ubx (Florence and McGinnis, 1998) nejQ7 ci (Florence and McGinnis, 1998) Dfd (Florence and McGinnis, 1998) qed (Florence, 1999.7.22) Ubx (Florence and McGinnis, 1998) nejS103 Dfd (Florence and McGinnis, 1998) Ubx (Florence and McGinnis, 1998) w (Petruk et al., 2001) nejS342 Dfd (Florence and McGinnis, 1998) Ubx (Florence and McGinnis, 1998) nejScer\UAS.cMa ash1 (Bantignies et al., 2000) nejScer\UAS.T:SV5\V5 so (Kumar et al., 2004) nejTA57 Dfd (Florence and McGinnis, 1998) nejunspecified Myb (Okada et al., 2002) nejWA69 Dfd (Florence and McGinnis, 1998) nejΔBHQ.Scer\UAS so (Kumar et al., 2004) unspecified ade2 (Anderson et al., 2005) CG1707 (Anderson et al., 2005) cgd (Anderson et al., 2005) CrebA (Anderson et al., 2005) CrebB-17A (Anderson et al., 2005) dpp (Anderson et al., 2005) Egfr (Anderson et al., 2005) enc (Anderson et al., 2005) ey (Anderson et al., 2005) gl (Anderson et al., 2005) grim (Mueller et al., 2005) H (Mueller et al., 2005) Iap2 (Anderson et al., 2005) kni (Anderson et al., 2005) Kr (Anderson et al., 2005) L (Singh et al., 2005) l(3)71DEc (Anderson et al., 2005) l(3)72Df (Anderson et al., 2005) l(3)L3659 (Anderson et al., 2005) lilli (Anderson et al., 2005) ll (Anderson et al., 2005) luc (Anderson et al., 2005) Mad (Kumar et al., 2004) Nsf2 (Laviolette et al., 2005) pan (Kumar et al., 2004) rho (Anderson et al., 2005) RhoGAP93B (Anderson et al., 2005) rn (Anderson et al., 2005) rpr (Mueller et al., 2005) Scr (Anderson et al., 2005) sog (Anderson et al., 2005) spi (Anderson et al., 2005) srp (Anderson et al., 2005) sty (Anderson et al., 2005) th (Anderson et al., 2005) Tpl (Anderson et al., 2005) Tsp42Ea (Anderson et al., 2005) Ubx (Florence and McGinnis, 1998, Anderson et al., 2005) W (Mueller et al., 2005) Xrp1 (Anderson et al., 2005)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261617 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43856
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22418 Dere\GG18962 Dere\GG18963 Dgri\GH12924 Dmoj\GI14627 Dper\GL16612 Dpse\GA13644 Dsec\GM11348 Dsec\GM11349 Dsim\GD16049 Dsim\GD16050 Dvir\GJ19060 Dwil\GK25575 Dyak\GE15437 Dyak\GE15438
Curated drosophilid orthologs	Dmir\CG15319 (Thornton, 2005.12.20) Dpse\GA13644
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB12963 NV12033 LH14963 ISCW021822 FBgn0233014 FBgn0227534 FBgn0206205 FBgn0187689 FBgn0166292 JGI_V11_64953 FBgn0073680 FBgn0154216 FBgn0137378 FBgn0120402 FBgn0111169 FBgn0099414 CPIJ005540 BGIBMGA000853 ACYPI003301 GB30193 GB12228 AGAP000029 AAEL017391 PHUM086710 GLEAN_08222
Stocks & Reagents
Stocks Listed in FlyBase ( 27 )
Bloomington	3729 w* nej3/FM7c 32573 w*; P{UAS-nej.wt-V5}3 34040 w* nej0.3/FM7c 3730 w*; P{hs-nej+}1 32576 P{UAS-nej.siRNA}hp12, w* 32574 w*; P{UAS-nej.F2161A-V5}2
Kyoto	107202 w* nej3/FM7c
VDRC	v102885 P{KK113191}VIE-260B v105115 P{KK101360}VIE-260B v3787 w1118; P{GD1494}v3787 v46534 w1118; P{GD8975}v46534 v46535 w1118; P{GD8975}v46535/TM3
	More stocks available...
Genomic Clones ( 2 )
	BACR21O19 BACR24L02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 49 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT16007 LD33277
Other clones	IP20061 IP20161
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT27654 GH08927 GM22496 LD05292 LD05355 LD13463 LD14330 LP22248 RE64320 RE65169 RH39189 RH53096 RH66781 RH68549
Other clones	29607 32961 59740 79695 104496 146843 160072 172610 214565.12 524501 525503 528069 CK01.39A.H4 CK01.92A.D3 CK01.109C.E9 CK00533 Dmel_neb_015457_0673_2156 EK071914 EK099017 EK142817 EK186314 EK194802 EK229233 EK249549 EN00407 EN06619 EN08449 EP11651 FGM001G04 RC0-DA0005-130700-011-B09 RC0-DL0001-140700-023-D07
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261617 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31911 43856 45160 45205 53481
Antibody Information
	polyclonal (Lilja et al., 2003, Zhao et al., 2009, Kirilly et al., 2011)
Other Information
Discoverer
Etymology
	'nejire' means 'twist' in Japanese. (Akimaru et al., 1997)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: nej anon-WO03040301.89 (FlyBase, 1996-) Source for merge of: nej CG15319 (Bayraktaroglu, 2000.4.12) Source for merge of: nej l(1)G0350 l(1)G0112 l(1)G0470 CG15321 (Yin et al., 2007)
Additional comments	Annotations CG15321 eliminated in release 5.25 of the genome annotation. (FlyBase Genome Annotators, 2010) FlyBase curator comment: "EP1410" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise. (Mueller et al., 2005) FlyBase curator comment: "EP950" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise. FlyBase curator comment: "EP1410" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise. (Singh et al., 2005) FlyBase curator comment: "EP950" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise. (Pena-Rangel et al., 2002) "l(1)G0464" may affect "nej". (Peter et al., 2002) "l(1)G0350" may affect "CG15321". (Peter et al., 2002) "l(1)G0112" may affect "CG15321". (Peter et al., 2002) "l(1)G0470" may affect "CG15321". (Peter et al., 2002) "l(1)G0350" may affect "CG15321". "l(1)G0286" may affect "CG15321". "l(1)G0137" may affect "CG15321". "l(1)G0112" may affect "CG15321". "l(1)G0270" may affect "CG15321". "l(1)G0470" may affect "CG15321". "l(1)G0236" may affect "CG15321". (Peter et al., 2002) Source for merge of nej anon-WO03040301.89 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Overexpression of nej can repress or activate wg signaling depending on the context. (Li et al., 2007) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders. (Inlow and Restifo, 2004) nej functions in eye specification. In addition, nej has a role in cell fate determination during eye development, in particular in R3/R4 cell fate choice and also probably in the specification of the R7 photoreceptor. (Kumar et al., 2004) nej and Myb work together to promote efficient progression through mitosis. (Fung et al., 2003) Reduction in maternally contributed nej protein levels in embryos leads to reductions in expression of tld, scw, tsg and rho, but not dpp, in early embryogenesis. rho expression is even lost in cells containing signal dependent phosphorylated Mad protein (a marker of BMP signaling). (Lilja et al., 2003) nej function is necessary for ci-mediated transactivation of wg during embryogenesis. (Chen et al., 2000) Postsynaptic nej is necessary for normal presynaptic functional development. (Marek et al., 2000) nej functions as a co-activator during dpp signalling. Mad may recruit nej to effect the transcriptional activation of dpp responsive genes during development. (Waltzer and Bienz, 1999) The nej gene product represses pan to antagonise wg signalling. (Waltzer and Bienz, 1998) Isolated from a genomic library using a fragment of the C.elegans CBP gene as a probe, under low stringency conditions. (Akimaru et al., 1997) nej has been cloned and characterised. (Akimaru et al., 1997) nej is necessary for dl-mediated activation of the twi promoter. (Akimaru et al., 1997) Using the co-transfection assay with the nej expression plasmid results demonstrate trans-activation by Myb and functional domains of Myb involved in this activity are identified. (Hou et al., 1997)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079903 NM_001201646 NM_001201647 RefSeq (Proteins) • NP_524642 NP_001188575 NP_001188576 DDBJ / EMBL / GenBank • AA979031 AAB53050 AAF46516 AAF46517 AAL13864 ADV37657 ADV37658 AJ426814 AJ426817 AJ426819 AQ025365 AQ025395 AQ025399 AQ025447 AW943174 AX189291 AX777235 AY058635 DQ327754 U88570 Entrez Gene - A searchable database of RefSeq genes. • 31911 43856 UniProtKB/TrEMBL • O01368 Q9W320 Q9W321 Q95TP4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Bromodomain, conserved site (IPR018359) Zinc finger, TAZ-type (IPR000197) Zinc finger, ZZ-type (IPR000433) Bromodomain (IPR001487) Coactivator CBP, KIX (IPR003101) Domain of unknown function DUF902, CREBbp (IPR010303) Nuclear receptor coactivator, interlocking (IPR009110) Zinc finger, PHD-type, conserved site (IPR019786) Nuclear receptor coactivator, CREB-bp-like, interlocking (IPR014744) Histone H3-K56 acetyltransferase, RTT109 (IPR013178)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261617 DRSC - Results from RNAi screens. • FBgn0261617 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015624 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261617 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31911 43856 45160 45205 53481 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43856 modMine - Data generated by the modENCODE project. • FBgn0261617 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB12963 NV12033 LH14963 ISCW021822 FBgn0233014 FBgn0227534 FBgn0206205 FBgn0187689 FBgn0166292 JGI_V11_64953 FBgn0073680 FBgn0154216 FBgn0137378 FBgn0120402 FBgn0111169 FBgn0099414 CPIJ005540 BGIBMGA000853 ACYPI003301 GB30193 GB12228 AGAP000029 AAEL017391 PHUM086710 GLEAN_08222
Synonyms & Secondary IDs ( 32 )
Reported As
Symbol Synonym	anon-WO0147981.11 (Modjtahedi, 2001.7.5) anon-WO03040301.89   CBP (Anderson et al., 2005, Yang, 2004, Sanchez-Elsner and Sauer, 2004, Smith et al., 2004, Ma, 2005, Lipsick, 2004, Kumar et al., 2004, Fernandez-Funez et al., 2005, Ogden et al., 2004, Fernandez-Funez et al., 2003, Ingham and McMahon, 2001, Sharpe et al., 2001, Novak and Dedhar, 1999, Goodman and Smolik, 2000, Matise and Joyner, 1999, Chen and Courey, 2000, Attisano and Wrana, 2000, Glass and Rosenfeld, 2000, Murone et al., 1999, Chen, 1997.2.5, Mannervik et al., 1999, Kouzarides, 1999, Ingham, 1998, Anonymous, 1998, Tabin and McMahon, 1997, Parker et al., 2007, Hung et al., 2007, Fortini, 2007, Tavantzis and Warrick, 2008, Eissenberg et al., 2005, Lim et al., 2007, Latouche et al., 2007, Yin et al., 2007, Parker et al., 2008, Parker et al., 2006, Wang et al., 2008, Fanti et al., 2008, Tie et al., 2009, Li et al., 2007, Mallik and Lakhotia, 2010, Reddy et al., 2010, Pruteanu-Malinici et al., 2011, Herz et al., 2010) cbp (Ayscough, 1998) Cbp (Chen et al., 1998, Chen et al., 1996) CBP_ (Bashirullah et al., 2006) CBP/p300 (Newfeld et al., 2002, Newfeld and Takaesu, 2002, Vo and Goodman, 2001, Seidensticker and Behrens, 2000, Behrens, 2000, Stadeli, 2006) CG15319 (Ramet et al., 2002, Laviolette et al., 2005, Grieder et al., 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Reddy et al., 2010, Ni et al., 2011.7.19) CG15321 (Yin et al., 2007, Grieder et al., 2007, Grieder et al., 2007, Stofanko et al., 2008, Keleman et al., 2009.8.5, FlyBase Genome Annotators, 2010, Manak et al., 2006, Popodi et al., 2010-, Ni et al., 2008) Crbp   dCBP (Smith et al., 2004, Fu et al., 2004, Evans et al., 2003, Maves and Schubiger, 2003, Li et al., 2004, Kumar et al., 2004, Lilja et al., 2003, Fung et al., 2003, Taylor et al., 2003, Cavarec et al., 2002, Simon and Tamkun, 2002, Newman et al., 2002, Li et al., 2003, Lilja et al., 2003, Petruk et al., 2001, Ludlam et al., 2001, Petruk et al., 2001, Bantignies et al., 2000, Marek et al., 2000, Ludlam et al., 2000, Davis et al., 2000, Chen et al., 2000, Roose and Clevers, 1999, Govind, 1999, Aza-Blanc and Kornberg, 1999, Vousden, 1999, Newman et al., 1999, Goodrich and Scott, 1998, Bantignies et al., 1999, Miska, 1998, Waltzer and Bienz, 1998, Giles et al., 1998, Bantignies et al., 1998, Anonymous, 1998, Akimaru et al., 1997, Hou et al., 1997, Florence and McGinnis, 1997, Akimaru et al., 1997, Fry and Peterson, 2001, Nibu et al., 2007, Nagraj et al., 2007, Petruk et al., 2006, Schuettengruber, 2006, Jung and Bonini, 2007, Fu and Ma, 2005, Ludlam et al., 2002, Tavantzis and Warrick, 2008, Smolik and Jones, 2007, Lilja et al., 2007, Hong et al., 2008, Jia et al., 2002, Zhao et al., 2009, Bantignies et al., 2002, Crump et al., 2011, Kwon et al., 2004) dKAT3 (Crump et al., 2011) dmCBP (Pandey et al., 2002) l(1)G0112   l(1)G0350   l(1)G0470   nej (DasGupta et al., 2005, Nagraj et al., 2007, Laviolette et al., 2005, Weber et al., 2007, Friedman and Perrimon, 2006, Ito et al., 2004, Grieder et al., 2007, Grieder et al., 2007, Hung et al., 2007, Gilfillan et al., 2007, Lilja et al., 2007, Stofanko et al., 2008, Ayroles et al., 2009, Straub et al., 2008, Dworkin et al., 2009, Li et al., 2007, Smolik and Jones, 2007, Pospisilik et al., 2010, He et al., 2010, Smolik, 2010.11.15, Smolik, 2011.2.28, Sela et al., 2010) Nej (Yin et al., 2007, Martin et al., 2007, Anholt and Williams, 2010, Guenin et al., 2010) nej CBP (Singh et al., 2005) Nejire (Reddy et al., 2010) p300 (Lee and Stallcup, 2006) p300/CBP (Mizzen and Allis, 2000, Lee and Stallcup, 2006)
Name Synonym	CBP/Nejire (Yin et al., 2007) CREB binding protein (Park et al., 2001, Ingham, 1998, Tavantzis and Warrick, 2008, Hart and Warrick, 2008, Yin et al., 2007, Zhao et al., 2009) CREB-binding protein (Johnston and Scott, 1998, Chen et al., 1996, Fortini, 2007, Parker et al., 2008) Drosophila CREB-binding (Kwon et al., 2004) Drosophila CREB-binding protein (Nibu et al., 2007) lethal (1) G0112   lethal (1) G0350   lethal (1) G0470   nejire (Akimaru et al., 1997, Olson et al., 2007, Pilot et al., 2006, Lilja et al., 2007, Stofanko et al., 2008, Wang et al., 2008, Fanti et al., 2008, Li et al., 2007, Mallik and Lakhotia, 2010) Nejire (Martin et al., 2007, Yin et al., 2007) protein (Kwon et al., 2004)
Secondary FlyBase IDs
	FBgn0015624 FBgn0027307 FBgn0028319 FBgn0030149 FBgn0030150 FBgn0040152 FBgn0066809
References ( 195 )
Generate a list of	All references relating to this gene ( 195 )
List References by type	Research paper  ( 84 ) Supplementary material  ( 9 ) Review  ( 41 ) Personal communication to FlyBase  ( 16 ) Abstract  ( 30 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 3 ) Conference report  ( 2 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Patent  ( 2 ) Curated genome annotation  ( 1 )
Recent research papers ( 12 )
	Crump et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(19): 7814--7819 Dynamic acetylation of all lysine-4 trimethylated histone H3 is evolutionarily conserved and mediated by p300/CBP. [FBrf0213668] Kirilly et al., 2011, Neuron 72(1): 86--100 Intrinsic epigenetic factors cooperate with the steroid hormone ecdysone to govern dendrite pruning in Drosophila. [FBrf0216319] Pruteanu-Malinici et al., 2011, PLoS Comput. Biol. 7(7): e1002098 Automatic Annotation of Spatial Expression Patterns via Sparse Bayesian Factor Models. [FBrf0214618] Schmidt et al., 2011, Glia 59(9): 1264--1272 Transcriptional regulation of peripheral glial cell differentiation in the embryonic nervous system of drosophila. [FBrf0214270] Anholt and Williams, 2010, Chem. Senses 35(1): 21--30 The soluble proteome of the Drosophila antenna. [FBrf0209552] Guenin et al., 2010, BMC Genomics 11: 47 Expression profiling of prospero in the Drosophila larval chemosensory organ: Between growth and outgrowth. [FBrf0210012] He et al., 2010, Biophys. J. 99(3): 697--707 Shaping a Morphogen Gradient for Positional Precision. [FBrf0211456] Herz et al., 2010, Mol. Cell. Biol. 30(10): 2485--2497 The H3K27me3 demethylase dUTX is a suppressor of notch- and Rb-dependent tumors in Drosophila. [FBrf0210661] Mallik and Lakhotia, 2010, Genetics 184(4): 927--945 Improved activities of CREB binding protein, heterogeneous nuclear ribonucleoproteins and proteasome following downregulation of noncoding hsromega transcripts help suppress poly(Q) pathogenesis in fly models. [FBrf0211742] Pospisilik et al., 2010, Cell 140(1): 148--160 Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. [FBrf0212676] Reddy et al., 2010, Mol. Cell. Biol. 30(21): 5234--5244 Drosophila Transcription Factor Tramtrack69 Binds MEP1 To Recruit the Chromatin Remodeler NuRD. [FBrf0212010] Sela et al., 2010, Genome Biol. 11(6): R59 The role of transposable elements in the evolution of non-mammalian vertebrates and invertebrates. [FBrf0214867] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...