Gene Dmel\nej

(Schaefer et al., 1999.12.2)

8F4-8F7

(determined by in situ hybridisation)

8F-9A

(Chen, 1997.2.5)

8F6-8F7

(determined by in situ hybridisation)

(Schaefer et al., 1999.8.12)

8F4-8F7

(determined by in situ hybridisation)

(Schaefer et al., 1999.6.15)

8F-9A

(determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\nej for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.

Gene model reviewed during 5.43

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

nej-RB

FBtr0071402

NM_079903

11733

3276

nej-RC

FBtr0302722

NM_001201646

11704

3282

nej-RD

FBtr0302723

NM_001201647

15729

3276

nej-RE

FBtr0333521

NM_001272458

15400

3266

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

nej-PB

FBpp0071337

340.7

3276

8.86

NP_524642

AAF46516

nej-PC

FBpp0291862

341.4

3282

8.89

NP_001188575

ADV37657

nej-PD

FBpp0291863

340.7

3276

8.86

NP_001188576

ADV37658

nej-PE

FBpp0305701

339.7

3266

8.86

NP_001259387

AGB95230

Additional Polypeptide Data & Comments

Reported size (kDa)

3190 (aa); 332 (kD)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Bromodomain, conserved site (IPR018359)

Zinc finger, TAZ-type (IPR000197)

Zinc finger, ZZ-type (IPR000433)

Bromodomain (IPR001487)

Coactivator CBP, KIX domain (IPR003101)

Domain of unknown function DUF902, CREBbp (IPR010303)

Nuclear receptor coactivator, interlocking (IPR009110)

Zinc finger, PHD-type, conserved site (IPR019786)

Histone H3-K56 acetyltransferase, RTT109 (IPR013178)

Nuclear receptor coactivator, CREB-bp-like, interlocking (IPR014744)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 12

organism | ubiquitous

(Ludlam et al., 2002)

embryonic stage 5

• nucleus

(Lilja et al., 2003)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(Marek et al., 2000, Bantignies et al., 2002)

References

nucleus

inferred from direct assay

protein complex

inferred from physical interaction with mod

(Bantignies et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, larval trachea, adult ovary, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		44
central nervous system, pupae P8		28
head, virgin 1-day female		13
head, virgin 4-day female		17
head, virgin 20-day female		6
head, mated 1-day female		16
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		4
digestive system, 4-day adult		9
digestive system, 20-day adult		6
fat body, larvae L3 wandering		6
fat body, white prepupae		12
fat body, pupae P8		7
carcass, larvae L3 wandering		22
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		6
ovary, virgin 4-day female		33
ovary, mated 4-day female		43
testis, mated 4-day male		5
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(28)
central nervous system, larvae L3		(44)
central nervous system, pupae P8		(28)
head, virgin 1-day female		13
head, virgin 4-day female		17
head, virgin 20-day female		6
head, mated 1-day female		16
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		4
digestive system, 4-day adult		9
digestive system, 20-day adult		6
fat body, larvae L3 wandering		6
fat body, white prepupae		12
fat body, pupae P8		7
carcass, larvae L3 wandering		22
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		6
ovary, virgin 4-day female		(33)
ovary, mated 4-day female		(43)
testis, mated 4-day male		5
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		44
central nervous system, pupae P8		28
head, virgin 1-day female		13
head, virgin 4-day female		17
head, virgin 20-day female		6
head, mated 1-day female		16
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		4
digestive system, 4-day adult		9
digestive system, 20-day adult		6
fat body, larvae L3 wandering		6
fat body, white prepupae		12
fat body, pupae P8		7
carcass, larvae L3 wandering		22
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		6
ovary, virgin 4-day female		33
ovary, mated 4-day female		43
testis, mated 4-day male		5
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		44
central nervous system, pupae P8		28
head, virgin 1-day female		13
head, virgin 4-day female		17
head, virgin 20-day female		6
head, mated 1-day female		16
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		4
digestive system, 4-day adult		9
digestive system, 20-day adult		6
fat body, larvae L3 wandering		6
fat body, white prepupae		12
fat body, pupae P8		7
carcass, larvae L3 wandering		22
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		6
ovary, virgin 4-day female		33
ovary, mated 4-day female		43
testis, mated 4-day male		5
accessory gland, mated 4-day male		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		44
central nervous system, pupae P8		28
head, virgin 1-day female		13
head, virgin 4-day female		17
head, virgin 20-day female		6
head, mated 1-day female		16
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		4
digestive system, 4-day adult		9
digestive system, 20-day adult		6
fat body, larvae L3 wandering		6
fat body, white prepupae		12
fat body, pupae P8		7
carcass, larvae L3 wandering		22
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		6
ovary, virgin 4-day female		33
ovary, mated 4-day female		43
testis, mated 4-day male		5
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		(44)
central nervous system, pupae P8		28
head, virgin 1-day female		13
head, virgin 4-day female		17
head, virgin 20-day female		6
head, mated 1-day female		16
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		4
digestive system, 4-day adult		9
digestive system, 20-day adult		6
fat body, larvae L3 wandering		6
fat body, white prepupae		12
fat body, pupae P8		7
carcass, larvae L3 wandering		22
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		6
ovary, virgin 4-day female		(33)
ovary, mated 4-day female		(43)
testis, mated 4-day male		5
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		44
central nervous system, pupae P8		28
head, virgin 1-day female		13
head, virgin 4-day female		17
head, virgin 20-day female		6
head, mated 1-day female		16
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		4
digestive system, 4-day adult		9
digestive system, 20-day adult		6
fat body, larvae L3 wandering		6
fat body, white prepupae		12
fat body, pupae P8		7
carcass, larvae L3 wandering		22
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		6
ovary, virgin 4-day female		33
ovary, mated 4-day female		43
testis, mated 4-day male		5
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		44
central nervous system, pupae P8		28
head, virgin 1-day female		13
head, virgin 4-day female		17
head, virgin 20-day female		6
head, mated 1-day female		16
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		1
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		4
digestive system, 4-day adult		9
digestive system, 20-day adult		6
fat body, larvae L3 wandering		6
fat body, white prepupae		12
fat body, pupae P8		7
carcass, larvae L3 wandering		22
carcass, 1-day adult		7
carcass, 4-day adult		10
carcass, 20-day adult		6
ovary, virgin 4-day female		33
ovary, mated 4-day female		43
testis, mated 4-day male		5
accessory gland, mated 4-day male		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 and 18-24 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		69
embryo 02-04hr		79
embryo 04-06hr		44
embryo 06-08hr		46
embryo 08-10hr		33
embryo 10-12hr		13
embryo 12-14hr		21
embryo 14-16hr		38
embryo 16-18hr		27
embryo 18-20hr		20
embryo 20-22hr		49
embryo 22-24hr		30
larva L1		12
larva L2		8
larva L3 12hr old		10
larva L3 puffstage 1-2		5
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		16
white prepupae 24hr		19
pupae 2d postWPP		12
pupae 3d postWPP		7
pupae 4d postWPP		8
adult male 01day		9
adult male 05day		12
adult male 30day		12
adult female 01day		27
adult female 05day		35
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(69)
embryo 02-04hr		(79)
embryo 04-06hr		(44)
embryo 06-08hr		(46)
embryo 08-10hr		(33)
embryo 10-12hr		13
embryo 12-14hr		21
embryo 14-16hr		(38)
embryo 16-18hr		(27)
embryo 18-20hr		20
embryo 20-22hr		(49)
embryo 22-24hr		(30)
larva L1		12
larva L2		8
larva L3 12hr old		10
larva L3 puffstage 1-2		5
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		16
white prepupae 24hr		19
pupae 2d postWPP		12
pupae 3d postWPP		7
pupae 4d postWPP		8
adult male 01day		9
adult male 05day		12
adult male 30day		12
adult female 01day		(27)
adult female 05day		(35)
adult female 30day		(38)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		69
embryo 02-04hr		79
embryo 04-06hr		44
embryo 06-08hr		46
embryo 08-10hr		33
embryo 10-12hr		13
embryo 12-14hr		21
embryo 14-16hr		38
embryo 16-18hr		27
embryo 18-20hr		20
embryo 20-22hr		49
embryo 22-24hr		30
larva L1		12
larva L2		8
larva L3 12hr old		10
larva L3 puffstage 1-2		5
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		16
white prepupae 24hr		19
pupae 2d postWPP		12
pupae 3d postWPP		7
pupae 4d postWPP		8
adult male 01day		9
adult male 05day		12
adult male 30day		12
adult female 01day		27
adult female 05day		35
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		69
embryo 02-04hr		79
embryo 04-06hr		44
embryo 06-08hr		46
embryo 08-10hr		33
embryo 10-12hr		13
embryo 12-14hr		21
embryo 14-16hr		38
embryo 16-18hr		27
embryo 18-20hr		20
embryo 20-22hr		49
embryo 22-24hr		30
larva L1		12
larva L2		8
larva L3 12hr old		10
larva L3 puffstage 1-2		5
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		16
white prepupae 24hr		19
pupae 2d postWPP		12
pupae 3d postWPP		7
pupae 4d postWPP		8
adult male 01day		9
adult male 05day		12
adult male 30day		12
adult female 01day		27
adult female 05day		35
adult female 30day		38
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		69
embryo 02-04hr		79
embryo 04-06hr		44
embryo 06-08hr		46
embryo 08-10hr		33
embryo 10-12hr		13
embryo 12-14hr		21
embryo 14-16hr		38
embryo 16-18hr		27
embryo 18-20hr		20
embryo 20-22hr		49
embryo 22-24hr		30
larva L1		12
larva L2		8
larva L3 12hr old		10
larva L3 puffstage 1-2		5
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		16
white prepupae 24hr		19
pupae 2d postWPP		12
pupae 3d postWPP		7
pupae 4d postWPP		8
adult male 01day		9
adult male 05day		12
adult male 30day		12
adult female 01day		27
adult female 05day		35
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(69)
embryo 02-04hr		(79)
embryo 04-06hr		(44)
embryo 06-08hr		(46)
embryo 08-10hr		(33)
embryo 10-12hr		13
embryo 12-14hr		21
embryo 14-16hr		(38)
embryo 16-18hr		27
embryo 18-20hr		20
embryo 20-22hr		(49)
embryo 22-24hr		30
larva L1		12
larva L2		8
larva L3 12hr old		10
larva L3 puffstage 1-2		5
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		16
white prepupae 24hr		19
pupae 2d postWPP		12
pupae 3d postWPP		7
pupae 4d postWPP		8
adult male 01day		9
adult male 05day		12
adult male 30day		12
adult female 01day		27
adult female 05day		(35)
adult female 30day		(38)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		69
embryo 02-04hr		79
embryo 04-06hr		44
embryo 06-08hr		46
embryo 08-10hr		33
embryo 10-12hr		13
embryo 12-14hr		21
embryo 14-16hr		38
embryo 16-18hr		27
embryo 18-20hr		20
embryo 20-22hr		49
embryo 22-24hr		30
larva L1		12
larva L2		8
larva L3 12hr old		10
larva L3 puffstage 1-2		5
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		16
white prepupae 24hr		19
pupae 2d postWPP		12
pupae 3d postWPP		7
pupae 4d postWPP		8
adult male 01day		9
adult male 05day		12
adult male 30day		12
adult female 01day		27
adult female 05day		35
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		69
embryo 02-04hr		79
embryo 04-06hr		44
embryo 06-08hr		46
embryo 08-10hr		33
embryo 10-12hr		13
embryo 12-14hr		21
embryo 14-16hr		38
embryo 16-18hr		27
embryo 18-20hr		20
embryo 20-22hr		49
embryo 22-24hr		30
larva L1		12
larva L2		8
larva L3 12hr old		10
larva L3 puffstage 1-2		5
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		13
white prepupae new		13
white prepupae 12hr		16
white prepupae 24hr		19
pupae 2d postWPP		12
pupae 3d postWPP		7
pupae 4d postWPP		8
adult male 01day		9
adult male 05day		12
adult male 30day		12
adult female 01day		27
adult female 05day		35
adult female 30day		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		10
embryonic 1182-4H		12
embryonic GM2		21
embryonic Kc167		12
embryonic S1		12
embryonic S3		18
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		17
wing disc ML-DmD9		26
wing disc ML-DmD16-c3		27
wing disc ML-DmD21		6
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		25
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		10
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		15
tumorous blood cells mbn2		20
ovary fGS/OSS		42
ovary OSC		38
ovary OSS		30
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(27)
Schneider line 2 Sg4		10
embryonic 1182-4H		12
embryonic GM2		21
embryonic Kc167		12
embryonic S1		12
embryonic S3		18
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		17
wing disc ML-DmD9		26
wing disc ML-DmD16-c3		(27)
wing disc ML-DmD21		6
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		25
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		10
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		15
tumorous blood cells mbn2		20
ovary fGS/OSS		(42)
ovary OSC		(38)
ovary OSS		(30)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		10
embryonic 1182-4H		12
embryonic GM2		21
embryonic Kc167		12
embryonic S1		12
embryonic S3		18
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		17
wing disc ML-DmD9		26
wing disc ML-DmD16-c3		27
wing disc ML-DmD21		6
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		25
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		10
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		15
tumorous blood cells mbn2		20
ovary fGS/OSS		42
ovary OSC		38
ovary OSS		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		10
embryonic 1182-4H		12
embryonic GM2		21
embryonic Kc167		12
embryonic S1		12
embryonic S3		18
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		17
wing disc ML-DmD9		26
wing disc ML-DmD16-c3		27
wing disc ML-DmD21		6
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		25
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		10
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		15
tumorous blood cells mbn2		20
ovary fGS/OSS		42
ovary OSC		38
ovary OSS		30
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		10
embryonic 1182-4H		12
embryonic GM2		21
embryonic Kc167		12
embryonic S1		12
embryonic S3		18
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		17
wing disc ML-DmD9		26
wing disc ML-DmD16-c3		27
wing disc ML-DmD21		6
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		25
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		10
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		15
tumorous blood cells mbn2		20
ovary fGS/OSS		42
ovary OSC		38
ovary OSS		30
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		10
embryonic 1182-4H		12
embryonic GM2		21
embryonic Kc167		12
embryonic S1		12
embryonic S3		18
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		17
wing disc ML-DmD9		26
wing disc ML-DmD16-c3		27
wing disc ML-DmD21		6
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		25
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		10
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		15
tumorous blood cells mbn2		20
ovary fGS/OSS		(42)
ovary OSC		(38)
ovary OSS		30
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		10
embryonic 1182-4H		12
embryonic GM2		21
embryonic Kc167		12
embryonic S1		12
embryonic S3		18
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		17
wing disc ML-DmD9		26
wing disc ML-DmD16-c3		27
wing disc ML-DmD21		6
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		25
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		10
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		15
tumorous blood cells mbn2		20
ovary fGS/OSS		42
ovary OSC		38
ovary OSS		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		10
embryonic 1182-4H		12
embryonic GM2		21
embryonic Kc167		12
embryonic S1		12
embryonic S3		18
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		17
wing disc ML-DmD9		26
wing disc ML-DmD16-c3		27
wing disc ML-DmD21		6
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		25
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		10
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		15
tumorous blood cells mbn2		20
ovary fGS/OSS		42
ovary OSC		38
ovary OSS		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		19
cold shock, 4-day adult		15
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		25
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		31
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		27
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		22
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		19
cold shock, 4-day adult		15
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		(40)
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		25
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		(31)
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(27)
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		22
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		19
cold shock, 4-day adult		15
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		25
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		31
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		27
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		22
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		19
cold shock, 4-day adult		15
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		25
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		31
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		27
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		22
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		19
cold shock, 4-day adult		15
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		25
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		31
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		27
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		22
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		19
cold shock, 4-day adult		15
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		(40)
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		25
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		31
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		27
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		22
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		19
cold shock, 4-day adult		15
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		25
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		31
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		27
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		22
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		19
cold shock, 4-day adult		15
heat shock, 4-day adult		13
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		40
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		25
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		31
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		27
Caffeine 25 mg/ml 48 hrs, 4-day adult		20
Paraquat 5 mM 48 hrs, 4-day adult		22
Paraquat 10 mM 48 hrs, 4-day adult		23
Rotenone 2 μg 12 hrs, larvae L3		1
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

External Data & Images

partially lethal - majority die | recessive

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015624

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal, with Scer\GAL4^pnr-MD237

nej^GD1494

lethal (with nej³)

nej^P

lethal (with nej^P)

lethal (with nej^Q7)

nej^P

lethal (with nej^S103)

nej^P

lethal (with nej^S342)

nej^P

lethal (with nej^TA57)

nej^P

lethal | adult stage

nej^G0112

lethal | embryonic stage | recessive

lethal | larval stage, with Scer\GAL4^elav.PLu

nej^EP1149

lethal | pharate adult stage

nej^G0112

lethal | pharate adult stage, with Scer\GAL4^30A

nej^Scer\UAS.cMa

lethal | prepupal stage

nej^G0470

lethal | prepupal stage, with Scer\GAL4^GMR.PF

nej^ΔQ.Scer\UAS

lethal | P-stage

lethal | pupal stage, with Scer\GAL4^Act5C.PI

nej^{dsRNA.Scer\UAS.cKa}

lethal | pupal stage, with Scer\GAL4^GMR.PF

nej^ΔQ.Scer\UAS

lethal | pupal stage, with Scer\GAL4^pnr-MD237

nej^GD8975

lethal | recessive

partially lethal - majority die | embryonic stage, with Scer\GAL4^Act5C.PI

nej^{dsRNA.Scer\UAS.cKa}

partially lethal - majority die | larval stage, with Scer\GAL4^Act5C.PI

nej^{dsRNA.Scer\UAS.cKa}

partially lethal - majority die | pharate adult stage, with Scer\GAL4^30A

nej^Scer\UAS.cMa

partially lethal - majority die | pharate adult stage, with Scer\GAL4^OK363

nej^Scer\UAS.cMa

partially lethal - majority live

viable, with Scer\GAL4[-]:

Sterility

Allele

male sterile

nej^G0350

Other Phenotypes

Allele

cell migration defective

nej^XII-13D1

developmental rate defective | recessive

developmental rate defective | recessive, with Scer\GAL4[-]:

eye color defective, with Scer\GAL4^GMR.PF

nej^{dsRNA.Scer\UAS.cKa}

gravitaxis defective | heat sensitive, with Scer\GAL4^hs.PU

nej^{dsRNA.Scer\UAS.cKa}

hypoactive | recessive

hypoactive | recessive, with Scer\GAL4[-]:

mitotic cell cycle defective | third instar larval stage, with Scer\GAL4^hs.PB

nej^{dsRNA.Scer\UAS}

neuroanatomy defective

neuroanatomy defective, with Scer\GAL4^elav.PLu

neuroanatomy defective | larval stage

nej^GD8975

neurophysiology defective

neurophysiology defective, with Scer\GAL4^elav.PLu

neurophysiology defective, with Scer\GAL4^Mhc.PW

nej^Scer\UAS.cMa

visible, with Scer\GAL4^30A

nej^Scer\UAS.cMa

visible, with Scer\GAL4^bbg-C96

visible, with Scer\GAL4^DBD.GMR

nej^Scer\UAS.cMa

visible, with Scer\GAL4^ey.PH

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^GMR.PF, nej³

visible, with Scer\GAL4^GMR.PF, nej^{ΔBHQ.Scer\UAS}

nej³

visible, with Scer\GAL4^GMR.PF, nej^{ΔNZK.Scer\UAS}

nej³

visible, with Scer\GAL4^hs.2sev

visible, with Scer\GAL4^hs.PB

nej^{dsRNA.Scer\UAS.cKa}

visible, with Scer\GAL4^OK363

nej^Scer\UAS.cMa

visible, with Scer\GAL4^OK386

nej^Scer\UAS.cMa

visible, with Scer\GAL4^pnr-MD237

nej^{dsRNA.Scer\UAS.cKa}

visible, with Scer\GAL4^sca-537.4

nej^EP1149

visible (with nej¹³¹)

nej^P

visible (with nej^P)

visible (with nej^TC41)

nej^P

visible | recessive | somatic clone

nej^TC41

visible | somatic clone

nej^8B27

wild-type

nej^hs.PA

Phenotype manifest in

Allele

abdominal 4 ventral denticle belt

nej¹

abdominal 5 ventral denticle belt

nej¹

adult abdominal segment 4 | ectopic, with Scer\GAL4^hs.PB

nej^{dsRNA.Scer\UAS.cKa}

adult abdominal segment 5, with Scer\GAL4^hs.PB

nej^{dsRNA.Scer\UAS.cKa}

amnioserosa | germline clone

nej¹

cephalopharyngeal skeleton

nej³

condensed chromosome | third instar larval stage, with Scer\GAL4^hs.PB

nej^{dsRNA.Scer\UAS}

cone cell | ectopic, with Scer\GAL4^GMR.PF

cuticle

nej³

denticle belt | anterior

nej³

dorsocentral bristle, with Scer\GAL4^sca-537.4

nej^EP1149

ectoderm | dorsal | germline clone

nej¹

embryonic/first instar larval cuticle

nej¹

embryonic/larval dorsal trunk

nej³

embryonic/larval midgut | embryonic stage

nej³

embryonic/larval midgut | embryonic stage | germline clone

nej¹

embryonic/larval proventriculus | embryonic stage

embryonic/larval salivary gland

embryonic/larval salivary gland, with Scer\GAL4^SG

nej^{dsRNA.Scer\UAS.cYa}

embryonic/larval tracheal system

nej³

embryonic/larval ventral anastomosis

nej³

embryonic antennal sense organ

embryonic head

nej¹

epidermis

eye, with Scer\GAL4^DBD.GMR

nej^Scer\UAS.cMa

eye, with Scer\GAL4^ey.PH

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^GMR.PF, nej³

eye, with Scer\GAL4^GMR.PF, nej^{ΔBHQ.Scer\UAS}

nej³

eye, with Scer\GAL4^GMR.PF, nej^{ΔNZK.Scer\UAS}

nej³

eye, with Scer\GAL4^hs.2sev

eye (with nej¹³¹)

nej^P

eye (with nej^P)

eye (with nej^TC41)

nej^P

eye | dorsal, with Scer\GAL4^ey.PH

nej^{ΔNZK.Scer\UAS}

eye | somatic clone

eye disc, with Scer\GAL4^ey.PH

nej^ΔQ.Scer\UAS

eye disc, with Scer\GAL4^GMR.PF

eye disc | somatic clone | third instar larval stage

nej^8B27

eye disc | third instar larval stage, with Scer\GAL4^hs.PB

nej^{dsRNA.Scer\UAS}

eye photoreceptor cell, with Scer\GAL4^GMR.PF

eye photoreceptor cell, with Scer\GAL4^hs.2sev

nej^ΔQ.Scer\UAS

eye photoreceptor cell (with nej¹³¹)

nej^P

eye photoreceptor cell (with nej^P)

nej¹³¹

eye photoreceptor cell | ectopic, with Scer\GAL4^GMR.PF

nej^{ΔNZK.Scer\UAS}

eye photoreceptor cell | somatic clone

nej^TC41

filzkorper

nej³

ganglion mother cell | larval stage

nej^GD8975

gastric caecum

nej³

head

interommatidial bristle, with Scer\GAL4^GMR.PF

interommatidial bristle, with Scer\GAL4^hs.2sev

nej^{ΔBHQ.Scer\UAS}

macrochaeta

nej³

median tooth

midgut constriction 1

nej³

mouth hooks

mystery cell, with Scer\GAL4^GMR.PF

nej^{ΔNZK.Scer\UAS}

neuromuscular junction

neuromuscular junction, with Scer\GAL4[-]:

neuromuscular junction, with Scer\GAL4^elav.PLu

neuromuscular junction, with Scer\GAL4^Mhc.PW

nej^Scer\UAS.cMa

neuromuscular junction, with Scer\GAL4^Mhc.PW, Scer\GAL4^elav.PLu

nej^EP1179

neuromuscular junction & bouton

neuromuscular junction & bouton, with Scer\GAL4[-]:

neuromuscular junction & bouton, with Scer\GAL4^elav.PLu

nej^EP1179

neuromuscular junction & t-bar, with Scer\GAL4[-]:

nej^EP1149

ommatidial precursor cluster, with Scer\GAL4^GMR.PF

ommatidium, with Scer\GAL4^ey.PH

nej^{dsRNA.Scer\UAS.cKa}

ommatidium, with Scer\GAL4^GMR.PF

ommatidium, with Scer\GAL4^hs.2sev

ommatidium (with nej¹³¹)

nej^P

ommatidium (with nej^P)

ommatidium (with nej^TC41)

nej^P

ommatidium | somatic clone

nej^TC41

outer photoreceptor cell | ectopic, with Scer\GAL4^hs.2sev

nej^KIX.Scer\UAS

photoreceptor cell R3, with Scer\GAL4^hs.2sev

photoreceptor cell R3 | ectopic, with Scer\GAL4^hs.2sev

photoreceptor cell R4, with Scer\GAL4^hs.2sev

photoreceptor cell R7, with Scer\GAL4^hs.2sev

photoreceptor cell R7 | ectopic, with Scer\GAL4^hs.2sev

pigment cell | somatic clone

nej^TC41

retina, with Scer\GAL4^GMR.PF

nej^{Scer\UAS.T:SV5\V5}

rhabdomere, with Scer\GAL4^GMR.PF

scutellar bristle, with Scer\GAL4^pnr-MD237

nej^{dsRNA.Scer\UAS.cKa}

scutellar bristle, with Scer\GAL4^sca-537.4

nej^EP1149

subperineurial glial cell

nej^XII-13D1

terminal organ | embryonic stage

thorax

tormogen cell, with Scer\GAL4^sca-537.4

nej^EP1149

trichogen cell, with Scer\GAL4^sca-537.4

nej^EP1149

wing, with Scer\GAL4^30A

nej^Scer\UAS.cMa

wing, with Scer\GAL4^bbg-C96

nej^{Scer\UAS.T:SV5\V5}

wing, with Scer\GAL4^OK363

nej^Scer\UAS.cMa

wing, with Scer\GAL4^OK386

nej^Scer\UAS.cMa

wing blade, with Scer\GAL4^bbg-C96

nej^{dsRNA.Scer\UAS.cKa}

wing margin, with Scer\GAL4^bbg-C96

Classical Alleles ( 28 )

For All Classical Alleles Show

Allele of nej	Class	Mutagen	Stocks	Known lesion
nej³		P-element activity	2	Yes
nej^G0119a			2	--
nej^Q7	antimorphic allele - genetic evidence	ethyl methanesulfonate	2	--
nej^0.3		ethyl methanesulfonate	1	--
nej^EP1179	hypomorphic allele - genetic evidence	P-element activity	1	Yes
nej^EP950		P-element activity	1	Yes
nej^G0112		P-element activity	1	--
nej^G0350		P-element activity	1	--
nej^G0470		P-element activity	1	--
nej^P		P-element activity	1	Yes
nej¹³¹			0	--
nej¹			0	--
nej²			0	--
nej^8B27		ethyl methanesulfonate	0	--
nej^CPTI000727		piggyBac transposase	0	Yes
nej^EP1149	hypomorphic allele - genetic evidence	P-element activity	0	Yes
nej^EP1410		P-element activity	0	Yes
nej^EP2246			0	--
nej^GG01399			0	--
nej^GS1188			0	--
nej^KG06619			0	--
nej^S103		ethyl methanesulfonate	0	--
nej^S342	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
nej^TA57		ethyl methanesulfonate	0	--
nej^TC41	hypomorphic allele - genetic evidence		0	--
nej^unspecified			0	--
nej^WA69		ethyl methanesulfonate	0	--
nej^XII-13D1		ethyl methanesulfonate	0	--

Alleles Carried on Transgenic Constructs ( 38 )

For All Alleles Carried on Transgenic Constructs Show

Allele of nej	Mutagen	Stocks	Known lesion
nej^{dsRNA.Scer\UAS}	in vitro construct - RNAi in vitro construct - regulatory fusion	3	Yes
nej^{F2161A.Scer\UAS.T:SV5\V5}	in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - coding region fusion	2	Yes
nej^GD8975	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
nej^+t16	in vitro construct - genomic fragment	1	Yes
nej^GD1494	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
nej^HM04037	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
nej^HMS01507	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
nej^HMS01570	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
nej^hs.PA	in vitro construct - regulatory fusion	1	Yes
nej^JF02806	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
nej^KK101360	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
nej^KK113191	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
nej^{Scer\UAS.T:SV5\V5}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	1	Yes
nej^+mSa	in vitro construct - minigene	0	Yes
nej^{1677-2608.Act5C.T:Ivir\HA1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
nej^{2040-3190.Act5C.T:Ivir\HA1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
nej^{383-1090.Act5C.T:Ivir\HA1}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
nej^Act5C.PA	in vitro construct - regulatory fusion	0	--
nej^CMV.cFa	in vitro construct - regulatory fusion	0	Yes
nej^dsRNA.cFa	in vitro construct - RNAi	0	Yes
nej^dsRNA.cRa	in vitro construct - RNAi	0	Yes
nej^{dsRNA.Scer\UAS.cKa}	in vitro construct - regulatory fusion in vitro construct - RNAi	0	Yes
nej^{dsRNA.Scer\UAS.cYa}	in vitro construct - regulatory fusion in vitro construct - RNAi	0	Yes
nej^{F2161A.MtnA.T:SV5\V5}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
nej^{HATmut.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - amino acid replacement	0	Yes
nej^KIX.Scer\UAS	in vitro construct - regulatory fusion in vitro construct - deletion	0	Yes
nej^Kr.PL	FLPase	0	Yes
nej^Kr.Scer\FRT	in vitro construct - regulatory fusion in vitro construct	0	Yes
nej^{MtnA.T:SV5\V5}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
nej^NIG.15319R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
nej^Scer\UAS.cMa	in vitro construct - regulatory fusion	0	Yes
nej^Scer\UAS.cYa	in vitro construct - regulatory fusion	0	Yes
nej^T:SV5\V5	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
nej^{Y2160A.Scer\UAS.T:SV5\V5}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
nej^{ΔBHQ.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - deletion	0	Yes
nej^{ΔHQ.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - deletion	0	Yes
nej^{ΔNZK.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - deletion	0	Yes
nej^ΔQ.Scer\UAS	in vitro construct - regulatory fusion in vitro construct - deletion	0	Yes

Aneuploid Aberrations

Not duplicated in

Dp(1;2)v⁺75d

Dp(1;3)w^m49a

Not disrupted in

Df(1)9a4-5

Df(1)dm75e19

Df(1)lz-90b24

Df(1)v-L15

(Popodi et al., 2010-, Venken et al., 2010)

Duplicated in

Dp(1;3)DC212

Dp(1;Y)FF1

Dp(1;Y)lz⁺

Disrupted in

Df(1)C52

P{Mae-UAS.6.11}B0457^B0457

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

heat-shock construct

characterization construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{Mae-UAS.6.11}B2010^B2010

P{Mae-UAS.6.11}B2011^B2011

P{Mae-UAS.6.11}B2012^B2012

P{Mae-UAS.6.11}GG01052^GG01052

P{Mae-UAS.6.11}nej^GG01399

miscellaneous insertions

P{SUPor-P}nej^KG06619

PBac{602.P.SVS-1}nej^CPTI000727

insertion of enhancer trap

P{lacW}l(1)G0137^G0137

P{lacW}l(1)G0236^G0236

P{lacW}l(1)G0270^G0270

P{lacW}l(1)G0286^G0286

P{lacW}l(1)G0400^G0400

P{lacW}l(1)G0464^G0464

Gene Ontology: Function, Process & Cellular Component ( 31 unique terms )

Terms Based on Experimental Evidence ( 26 terms )

Molecular Function

CV term

histone acetyltransferase activity

References

inferred from direct assay

(Ludlam et al., 2002)

histone acetyltransferase activity (H3-K18 specific)

inferred from direct assay

histone acetyltransferase activity (H3-K27 specific)

inferred from direct assay

protein binding

inferred from physical interaction with Clk

(Bantignies et al., 2002)

inferred from physical interaction with mod

transcription coactivator activity

inferred from direct assay

inferred from genetic interaction with ci

inferred from genetic interaction with per

transcription factor binding

inferred from physical interaction with ci

Biological Process

CV term

circadian regulation of gene expression

References

inferred from mutant phenotype

(Mallik and Lakhotia, 2010)

compound eye development

inferred from mutant phenotype

compound eye morphogenesis

inferred from mutant phenotype

DNA replication checkpoint

inferred from mutant phenotype

(Smolik and Jones, 2007)

glial cell migration

inferred from mutant phenotype

(Schmidt et al., 2011)

histone H3-K18 acetylation

inferred from direct assay

histone H3-K27 acetylation

inferred from direct assay

histone H4-K8 acetylation

inferred from mutant phenotype

(Ludlam et al., 2002, Boyles et al., 2010)

histone H4-K12 acetylation

inferred from mutant phenotype

(Ludlam et al., 2002)

locomotor rhythm

inferred from mutant phenotype

neurotransmitter secretion

neurogenesis

inferred from mutant phenotype

(Neumüller et al., 2011)

inferred from mutant phenotype

positive regulation of transcription from RNA polymerase II promoter

inferred from genetic interaction with ci

inferred from mutant phenotype

R3/R4 cell fate commitment

inferred from mutant phenotype

R7 cell differentiation

inferred from mutant phenotype

smoothened signaling pathway

regulation of mitosis

inferred from genetic interaction with Myb AND inferred from mutant phenotype

(Fung et al., 2003)

inferred from mutant phenotype

synapse assembly

inferred from mutant phenotype

Cellular Component

CV term

(Marek et al., 2000, Bantignies et al., 2002)

References

nucleus

inferred from direct assay

protein complex

inferred from physical interaction with mod

(Bantignies et al., 2002)

Terms Based on Predictions or Assertions ( 8 terms )

Molecular Function

CV term

cAMP response element binding protein binding

References

traceable author statement

transcription coactivator activity

inferred from sequence or structural similarity

(Chen et al., 1996)

inferred from sequence or structural similarity with mouse Crebbp

(FlyBase, 1992-)

zinc ion binding

inferred from electronic annotation with InterPro:IPR000197, InterPro:IPR000433

(FlyBase Curators et al., 2004-)

Biological Process

CV term

smoothened signaling pathway

References

hemopoiesis

traceable author statement

(Evans et al., 2003)

non-traceable author statement

(Fry and Peterson, 2001)

Wnt receptor signaling pathway

non-traceable author statement

(Fry and Peterson, 2001)

Cellular Component

CV term

histone acetyltransferase complex

References

inferred from electronic annotation with InterPro:IPR014744

(FlyBase Curators et al., 2004-)

nucleus

inferred from sequence or structural similarity

(Chen et al., 1996)

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

nej - His3

histone acetylation assay, western blot

(Tie et al., 2012)

nej - Hrb87F

anti bait coimmunoprecipitation, western blot

(Mallik and Lakhotia, 2010)

nej - Utx

anti bait coimmunoprecipitation, western blot, pull down, molecular sieving, anti tag western blot

(Tie et al., 2012)

nej - bl

anti bait coimmunoprecipitation, western blot

(Mallik and Lakhotia, 2010)

nej - brm

anti bait coimmunoprecipitation, western blot, pull down, molecular sieving, anti tag western blot

(Tie et al., 2012)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

nej allele

Gene

References

nej^Q7

qed

(Florence, 1999.7.22)

nej^S103

(Petruk et al., 2001)

nej^S342

(Bantignies et al., 2000)

nej^Scer\UAS.cMa

ash1

nej^{Scer\UAS.T:SV5\V5}

nej^TA57

nej^{ΔBHQ.Scer\UAS}

unspecified

ade2

CG1707

cgd

CrebA

CrebB

dpp

Egfr

enc

grim

Iap2

kni

(Singh et al., 2005)

l(3)71DEc

l(3)72Df

l(3)L3659

lilli

luc

Mad

(Laviolette et al., 2005)

Nsf2

pan

rho

RhoGAP93B

rpr

Scr

sog

spi

srp

sty

Tpl

Tsp42Ea

(Florence and McGinnis, 1998, Anderson et al., 2005)

Xrp1

External Data

P{UAS-nej.siRNA}hp12, w^*

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0261617

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

43856

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

O01368

Orthologs

OrthoDB Orthologs (73) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6MKP1T

Dipteran inclusive ortholog search

EOG6NZV2H

Insect inclusive ortholog search

EOG6MW727

Arthropod inclusive ortholog search

EOG61G1K8

Metazoa inclusive ortholog search

EOG60PH0T

Orthologs in Drosophila Species (EOG6MKP1T)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila yakuba

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13644

Drosophila pseudoobscura pseudoobscura

Dpse\GA28845

Drosophila persimilis

Dper\GL16612

Drosophila willistoni

Dwil\GK25575

Drosophila willistoni

Drosophila virilis

Drosophila virilis

Drosophila virilis

Drosophila mojavensis

Dmoj\GI19568

Drosophila mojavensis

Dmoj\GI14627

Drosophila grimshawi

Dgri\GH12924

Orthologs in non-Drosophila Dipterans (EOG6NZV2H)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL017391

Anopheles gambiae

Malaria mosquito

Agam\CPR124

Anopheles gambiae

Malaria mosquito

Agam\AGAP000674

Anopheles gambiae

Malaria mosquito

Agam\AGAP004987

Anopheles gambiae

Malaria mosquito

Agam\AGAP013080

Anopheles gambiae

Malaria mosquito

Agam\AGAP000029

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005540

Orthologs in non-Dipteran Insects (EOG6MW727)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB55583

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG014815

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004485

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH12674

Atta cephalotes

Leafcutter ant

Acep\ACEP18558

Atta cephalotes

Leafcutter ant

Acep\ACEP18556

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO14001

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL13451

Linepithema humile

Argentine ant

Lhum\LH14963

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB12963

Solenopsis invicta

Red fire ant

Sinv\SINV18831

Solenopsis invicta

Red fire ant

Sinv\SINV19895

Solenopsis invicta

Red fire ant

Sinv\SINV23370

Solenopsis invicta

Red fire ant

Sinv\SINV24002

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003301

Bombyx mori

Silkmoth

Bmor\BGIBMGA000853

Pediculus humanus

Human body louse

Phum\PHUM086710

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_08222

Orthologs in non-Insect Arthropods (EOG61G1K8)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_64953

Ixodes scapularis

Deer tick

Isca\ISCW021822

Orthologs in non-Arthropod Metazoa (EOG60PH0T)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\R10E11.1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_019024gn

Danio rerio

Zebrafish

Drer\ep300a

Danio rerio

Zebrafish

Drer\crebbpb

Danio rerio

Zebrafish

Drer\crebbpa

Danio rerio

Zebrafish

Drer\ep300b

Danio rerio

Zebrafish

Drer\ENSDARG00000060976

Xenopus tropicalis

Western clawed frog

Xtro\crebbp

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000022974

Gallus gallus

Domestic chicken

Ggal\EP300

Gallus gallus

Domestic chicken

Ggal\CREBBP

Mus musculus

House mouse

Mmus\Crebbp

Mus musculus

House mouse

Mmus\Ep300

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000000190

Rattus norvegicus

Norway rat

Rnor\Crebbp

Homo sapiens

Human

Hsap\CREBBP

Homo sapiens

Human

Hsap\EP300

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\CREBBP

Hsap\EP300

AAA Orthologs (15) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13644

Drosophila persimilis

Dper\GL16612

Drosophila willistoni

Dwil\GK25575

Drosophila virilis

Dvir\GJ19060

Drosophila mojavensis

Dmoj\GI14627

Drosophila grimshawi

Dgri\GH12924

Stocks & Reagents

Stocks Listed in FlyBase ( 29 )

Bloomington

3729

w^* nej³/FM7c

32573

w^*; P{UAS-nej.wt-V5}3

34040

w^* nej^0.3/FM7c

3730

w^*; P{hs-nej⁺}1

32576

32574

w^*; P{UAS-nej.F2161A-V5}2

32577

w^*; P{UAS-nej.siRNA}hp12.3

107202

v102885

v105115

v3787

w¹¹¹⁸; P{GD1494}v3787

v46534

w¹¹¹⁸; P{GD8975}v46534

More stocks available...

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 49 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

(Holmqvist et al., 2012, Lilja et al., 2003, Zhao et al., 2009, Kirilly et al., 2011)

DRSC - Results from RNAi screens.

•

FBgn0261617

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

43856

Antibody Information

polyclonal

Other Information

Discoverer

Etymology

'nejire' means 'twist' in Japanese.

(Bayraktaroglu, 2000.4.12)

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: nej anon-WO03040301.89

(FlyBase, 1996-)

Source for merge of: nej CG15319

Source for merge of: nej l(1)G0350 l(1)G0112 l(1)G0470 CG15321

(Yin et al., 2007)

Additional comments

Annotations CG15321 eliminated in release 5.25 of the genome annotation.

(FlyBase Genome Annotators, 2010)

FlyBase curator comment: "EP1410" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

(Pena-Rangel et al., 2002)

FlyBase curator comment: "EP950" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise. FlyBase curator comment: "EP1410" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

(Singh et al., 2005)

FlyBase curator comment: "EP950" overexpression phenotype stated to be due to its effect on nej but orientation of the P{EP} element suggests otherwise.

"l(1)G0464" may affect "nej".

"l(1)G0350" may affect "CG15321".

"l(1)G0112" may affect "CG15321".

"l(1)G0470" may affect "CG15321".

"l(1)G0350" may affect "CG15321". "l(1)G0286" may affect "CG15321". "l(1)G0137" may affect "CG15321". "l(1)G0112" may affect "CG15321". "l(1)G0270" may affect "CG15321". "l(1)G0470" may affect "CG15321". "l(1)G0236" may affect "CG15321".

(Inlow and Restifo, 2004)

Source for merge of nej anon-WO03040301.89 was sequence comparison (date:051113).

(FlyBase, 1996-)

Other Comments

Overexpression of nej can repress or activate wg signaling depending on the context.

(Li et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.

nej functions in eye specification. In addition, nej has a role in cell fate determination during eye development, in particular in R3/R4 cell fate choice and also probably in the specification of the R7 photoreceptor.

nej and Myb work together to promote efficient progression through mitosis.

(Fung et al., 2003)

Reduction in maternally contributed nej protein levels in embryos leads to reductions in expression of tld, scw, tsg and rho, but not dpp, in early embryogenesis. rho expression is even lost in cells containing signal dependent phosphorylated Mad protein (a marker of BMP signaling).

(Lilja et al., 2003)

nej function is necessary for ci-mediated transactivation of wg during embryogenesis.

Postsynaptic nej is necessary for normal presynaptic functional development.

(Waltzer and Bienz, 1999)

nej functions as a co-activator during dpp signalling. Mad may recruit nej to effect the transcriptional activation of dpp responsive genes during development.

The nej gene product represses pan to antagonise wg signalling.

(Waltzer and Bienz, 1998)

Isolated from a genomic library using a fragment of the C.elegans CBP gene as a probe, under low stringency conditions.

nej has been cloned and characterised.

nej is necessary for dl-mediated activation of the twi promoter.