FB2020_03, released Jun 16, 2020

Gene: Dmel\tweek

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General Information
Symbol
Dmel\tweek
Species
D. melanogaster
Name
tweek
Annotation Symbol
CG42555
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261671
Gene Model Status
Current
Stock Availability
15 publicly available
Gene Snapshot
tweek (tweek) encodes a protein involved in the endocytosis of synaptic vesicles. [Date last reviewed: 2019-09-19]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
36B4-36B4
Sequence location
2L:16,888,490..16,917,052 [+]
Recombination map

2-53

RefSeq locus
NT_033779 REGION:16888490..16917052
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (1 term)
Molecular Function (0 terms)
There is no information supporting a Molecular Function term for this gene. (Based on a review of available data on: 2009-02-19 )
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
synaptic vesicle endocytosis
inferred from mutant phenotype
(Verstreken et al., 2009)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
synaptic vesicle endocytosis
inferred from biological aspect of ancestor with PANTHER:PTN000781310
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
6

Please see the GBrowse view of Dmel\tweek or the JBrowse view of Dmel\tweek for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Shares 5' exon(s) with upstream non-coding gene; shared promoter

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
gene
gene_with_edited_transcript
(Rodriguez et al., 2012)
nuclear_gene
(FlyBase Genome Annotators, 2008)
protein_coding_gene
(FlyBase Genome Annotators, 2008)
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
tweek-RF
FBtr0304927
NM_001201896
16289
5095
tweek-RG
FBtr0304928
NM_001201895
16292
5096
tweek-RH
FBtr0304929
NM_001201897
16352
5116
tweek-RI
FBtr0304930
NM_001201898
16229
5075
tweek-RJ
FBtr0340172
NM_001299073
16454
5150
tweek-RK
FBtr0340173
NM_001299074
16334
5110
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
tweek-PF
FBpp0293466
566.9
5095
7.38
NP_001188825
ADV37074
tweek-PG
FBpp0293467
567.1
5096
7.33
NP_001188824
ADV37073
tweek-PH
FBpp0293468
569.3
5116
7.39
NP_001188826
ADV37075
tweek-PI
FBpp0293469
564.8
5075
7.32
NP_001188827
ADV37076
tweek-PJ
FBpp0309157
573.2
5150
7.44
NP_001286002
AHN54516
tweek-PK
FBpp0309158
569.0
5110
7.54
NP_001286003
AHN54517
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tweek using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 15
embryonic/larval midgut primordium | restricted
(Verstreken et al., 2009)
embryonic hindgut | restricted
(Verstreken et al., 2009)
embryonic brain | restricted
(Verstreken et al., 2009)
ventral nerve cord | restricted
(Verstreken et al., 2009)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\tweek in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 10 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of tweek
Transgenic constructs containing regulatory region of tweek
Deletions and Duplications ( 2 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(2L)Exel8036)
lethal (with tweek2)
lethal (with tweekc01084)
lethal (with tweekEY02585-Δ1)
lethal | first instar larval stage | recessive
lethal | pharate adult stage (with tweek2)
lethal | pharate adult stage (with tweekEY02585-Δ1)
lethal | recessive | second instar larval stage
lethal - all die before end of P-stage (with Df(2L)Exel8036)
lethal - all die before end of P-stage (with tweek1)
lethal - all die before end of P-stage (with tweek2)
lethal - all die before end of P-stage (with tweekc01084)
lethal - all die before end of P-stage | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
embryonic/larval segmentation phenotype
neurophysiology defective (with Df(2L)Exel8036)
neurophysiology defective (with tweek1)
neurophysiology defective (with tweek2)
neurophysiology defective (with tweekc01084)
neurophysiology defective | somatic clone
some die during embryonic stage
some die during pharate adult stage (with Df(2L)Exel8036)
some die during pharate adult stage (with tweek1)
some die during pharate adult stage (with tweek2)
some die during pharate adult stage (with tweekc01084)
some die during pharate adult stage | recessive
uncoordinated | adult stage
uncoordinated | adult stage (with Df(2L)Exel8036)
uncoordinated | adult stage (with tweek1)
uncoordinated | adult stage (with tweek2)
uncoordinated | adult stage (with tweekc01084)
uncoordinated | adult stage (with tweekEY02585-Δ1)
Phenotype manifest in
Allele
embryonic/first instar larval cuticle
embryonic/larval neuromuscular junction | somatic clone | third instar larval stage (with tweek1)
embryonic/larval neuromuscular junction | somatic clone | third instar larval stage (with tweek2)
optic cartridge | somatic clone
synaptic vesicle (with Df(2L)Exel8036)
synaptic vesicle | somatic clone
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\KIAA1109
11 of 15
Yes
Yes
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\4932438A13Rik
11 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\RGD1307100
8 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\kiaa1109
5 of 12
Yes
Yes
Xtro\LOC101733939
2 of 12
No
Yes
Xtro\LOC101734304
2 of 12
No
Yes
Xtro\LOC100037856
1 of 12
No
Yes
Danio rerio (Zebrafish) (1)
Drer\si:ch211-233a24.2
9 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\lpd-3
9 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\CSF1
4 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919000F )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001084
Drosophila simulans
Dsim\GD24095
Drosophila sechellia
Dsec\GM17219
Drosophila erecta
Dere\GG21070
Drosophila yakuba
Dyak\GE12779
Drosophila ananassae
Dana\GF14779
Drosophila pseudoobscura pseudoobscura
Dpse\GA13520
Drosophila persimilis
Dper\GL14210
Drosophila willistoni
Dwil\GK23792
Drosophila virilis
Dvir\GJ16182
Drosophila mojavensis
Dmoj\GI17140
Drosophila grimshawi
Dgri\GH11695
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915000D )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17014109
Glossina morsitans
Tsetse fly
Gmor\GMOY007018
Glossina morsitans
Tsetse fly
Gmor\GMOY000590
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_06938
Mayetiola destructor
Hessian fly
Mdes\Mdes015329
Mayetiola destructor
Hessian fly
Mdes\Mdes014139
Mayetiola destructor
Hessian fly
Mdes\Mdes009409
Mayetiola destructor
Hessian fly
Mdes\Mdes012385
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL018032
Anopheles darlingi
American malaria mosquito
Adar\ADAC002372
Anopheles gambiae
Malaria mosquito
Agam\AGAP007960
Anopheles gambiae
Malaria mosquito
Agam\AGAP007961
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001558
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001559
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W000U )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA003784
Bombyx mori
Silkmoth
Bmor\BGIBMGA003783
Danaus plexippus
Monarch butterfly
Dple\DPOGS210934-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL003956
Apis florea
Little honeybee
Aflo\2606849444
Apis mellifera
Western honey bee
Amel\GB52098
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP25757
Bombus terrestris
Buff-tailed bumblebee
Bter\15213325
Linepithema humile
Argentine ant
Lhum\LH16800
Linepithema humile
Argentine ant
Lhum\LH10574
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00073f
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG025076
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG029264
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_09412
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_09410
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_09416
Tribolium castaneum
Red flour beetle
Tcas\TC033411
Pediculus humanus
Human body louse
Phum\PHUM335460
Rhodnius prolixus
Kissing bug
Rpro\RPRC005634
Cimex lectularius
Bed bug
Clec\CLEC002986
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006956
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_05240
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X000T )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR009191
Ixodes scapularis
Black-legged tick
Isca\ISCW000089
Ixodes scapularis
Black-legged tick
Isca\ISCW024023
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73899.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73894.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73890.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73895.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73892.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM73900.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur13g01930
Tetranychus urticae
Two-spotted spider mite
Turt\tetur13g01920
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_328986
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G004E )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hypp_1675
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_007475gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_000945gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_002062gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Kiaa1109L_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Kiaa1109L
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_009369.1gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000011515
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000005880
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000021260
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000011516
Gallus gallus
Domestic chicken
Ggal\KIAA1109
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG42556
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    KIAA1109; KIAA1109
    model of  Alkuraya-Kucinskas syndrome
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KIAA1109; KIAA1109
    11 of 15
    611565
     
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map

    2-53

    Cytogenetic map
    36B4-36B4
    Sequence location
    2L:16,888,490..16,917,052 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    36B4-36B4
    Limits computationally determined from genome sequence between P{lacW}Mhck10423&P{lacW}Cask03902 and P{lacW}Aac11k06710
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    36A-36A
    (Kulkarni, 2002.2.28)
    Experimentally Determined Recombination Data
    Location

    2-53

    (FlyBase, 2016)

    2-51.6

    (Comeron et al., 2012)
    Left of (cM)
    (Verstreken et al., 2009)
    Right of (cM)
    (Verstreken et al., 2009)
    Notes
    Stocks and Reagents
    Stocks (15)
    Genomic Clones (17)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (9)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of
            Source for database merge of

            Source for merge of: tweek CG42555 CG15134 CG4841

            (Verstreken et al., 2009)
            Additional comments

            Annotations CG42555, CG15134 and CG4841 merged as CG42706 in release 5.26 of the genome annotation.

            (FlyBase Genome Annotators, 2010)

            Annotation CG15133 split into CG42555 and CG42556 in release 5.16 of the genome annotation.

            (FlyBase Genome Annotators, 2008)
            Other Comments
            Origin and Etymology
            Discoverer
            Etymology

            The gene is named "tweek" as the behaviour of rare mutant adult escapers is reminiscent of the cartoon character "Tweek" from the TV series "South Park".

            (Verstreken et al., 2009)

            Named after a common street name for methamphetamine, because mutant escaper flies are severely uncoordinated.

            (Verstreken and Bellen, 2006)
            Identification
            External Crossreferences and Linkouts ( 62 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMine - An integrated database for Drosophila genomics
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Synonyms and Secondary IDs (7)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (40)