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Gene Dmel\tweek

General Information
Symbol	Dmel\tweek	Species	D. melanogaster
Name	tweek	Annotation symbol	CG42555
Feature type	protein_coding_gene	FlyBase ID	FBgn0261671
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	CG4841, CG15133, CG15134
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	36B4-36B4	Sequence location	2L:16,888,490..16,917,052 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene tweek is referred to in FlyBase by the symbol Dmel\tweek (CG42555, FBgn0261671). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: synaptic vesicle endocytosis. 13 alleles are reported. The phenotype of these alleles is annotated with: synaptic vesicle. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: Fragile site-associated protein, C-terminal; Methylmalonic aciduria and homocystinuria type D protein. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed during late larval stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval fat body. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. Gene sequence location is 2L:16888490..16917052.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 36B4-36B4   Limits computationally determined from genome sequence between P{lacW}Mhck10423&P{lacW}Cask03902 and P{lacW}Aac11k06710
Experimentally Determined Cytological Location
Cytogenetic map Notes References 36A-36A     (Kulkarni, 2002.2.28)
Experimentally Determined Recombination Data
Location
Left of (cM)	P{SUPor-P}KG05250 (Verstreken et al., 2009)
Right of (cM)	P{EPgy2}CG31739EY12630 (Verstreken et al., 2009)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\tweek for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50 Gene split based on EST/cDNA data. Gene merge based on protein alignment (BLASTX) data and RNAseq data. Shares non-coding sequences: 5' UTR sequences overlap a non-coding gene on the same strand. Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) tweek-RF FBtr0304927  NM_001201896   16289   5095 tweek-RG FBtr0304928  NM_001201895   16292   5096 tweek-RH FBtr0304929  NM_001201897   16352   5116 tweek-RI FBtr0304930  NM_001201898   16229   5075 tweek-RJ FBtr0340172     16454   5150 tweek-RK FBtr0340173     16334   5110
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein tweek-PF   FBpp0293466   566.9   5095   7.38     NP_001188825   ADV37074 tweek-PG   FBpp0293467   567.1   5096   7.33     NP_001188824   ADV37073 tweek-PH   FBpp0293468   569.3   5116   7.39     NP_001188826   ADV37075 tweek-PI   FBpp0293469   564.8   5075   7.32     NP_001188827   ADV37076 tweek-PJ   FBpp0309157   573.2   5150   7.44       tweek-PK   FBpp0309158   569.0   5110   7.54
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Methylmalonic aciduria and homocystinuria type D protein (IPR019362) Fragile site-associated protein, C-terminal (IPR018863)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567559   AE014134 ADV37073   AE014134 ADV37074   AE014134 ADV37075   AE014134 ADV37076   AF489106 AAM03127   AI516845   AW941059   BF501979   BF502442   BI573991   BI590247   CO184856   CO268808   CO277578   CO294127   CO302530   CO340478   CO342270   CO342855   CO343103   CO344793   CZ488099   EC074312   EC208903   EC212669   EC220325   EC243037   EC243700   EC252077   EC253546   EC255143   EC256100   EV597522   EW712858   EW712978   FL502728   FL502729   GH903147   GR705847
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VJG3 Q9VJF9 B7YZW5 Q8T620 Q9VJG1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15   embryonic/larval midgut primordium | restricted   (Verstreken et al., 2009) embryonic hindgut | restricted   (Verstreken et al., 2009) embryonic brain | restricted   (Verstreken et al., 2009) ventral nerve cord | restricted   (Verstreken et al., 2009)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval fat body. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    16 central nervous system, pupae P8    16 head, virgin 1-day female    6 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    11 head, mated 4-day male    11 head, mated 20-day male    12 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    4 fat body, larvae L3 wandering    11 fat body, white prepupae    21 fat body, pupae P8    5 carcass, larvae L3 wandering    10 carcass, 1-day adult    5 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    16 central nervous system, pupae P8    16 head, virgin 1-day female    6 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    11 head, mated 4-day male    11 head, mated 20-day male    12 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    4 fat body, larvae L3 wandering    11 fat body, white prepupae    21 fat body, pupae P8    5 carcass, larvae L3 wandering    10 carcass, 1-day adult    5 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    16 central nervous system, pupae P8    16 head, virgin 1-day female    6 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    11 head, mated 4-day male    11 head, mated 20-day male    12 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    4 fat body, larvae L3 wandering    11 fat body, white prepupae    21 fat body, pupae P8    5 carcass, larvae L3 wandering    10 carcass, 1-day adult    5 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    16 central nervous system, pupae P8    16 head, virgin 1-day female    6 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    11 head, mated 4-day male    11 head, mated 20-day male    12 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    4 fat body, larvae L3 wandering    11 fat body, white prepupae    21 fat body, pupae P8    5 carcass, larvae L3 wandering    10 carcass, 1-day adult    5 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    16 central nervous system, pupae P8    16 head, virgin 1-day female    6 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    11 head, mated 4-day male    11 head, mated 20-day male    12 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    4 fat body, larvae L3 wandering    11 fat body, white prepupae    21 fat body, pupae P8    5 carcass, larvae L3 wandering    10 carcass, 1-day adult    5 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    16 central nervous system, pupae P8    16 head, virgin 1-day female    6 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    11 head, mated 4-day male    11 head, mated 20-day male    12 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    4 fat body, larvae L3 wandering    11 fat body, white prepupae    21 fat body, pupae P8    5 carcass, larvae L3 wandering    10 carcass, 1-day adult    5 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    16 central nervous system, pupae P8    16 head, virgin 1-day female    6 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    11 head, mated 4-day male    11 head, mated 20-day male    12 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    4 fat body, larvae L3 wandering    11 fat body, white prepupae    21 fat body, pupae P8    5 carcass, larvae L3 wandering    10 carcass, 1-day adult    5 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    16 central nervous system, pupae P8    16 head, virgin 1-day female    6 head, virgin 4-day female    7 head, virgin 20-day female    8 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    11 head, mated 4-day male    11 head, mated 20-day male    12 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    4 fat body, larvae L3 wandering    11 fat body, white prepupae    21 fat body, pupae P8    5 carcass, larvae L3 wandering    10 carcass, 1-day adult    5 carcass, 4-day adult    4 carcass, 20-day adult    4 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed during late larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    9 embryo 02-04hr    11 embryo 04-06hr    14 embryo 06-08hr    13 embryo 08-10hr    14 embryo 10-12hr    7 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    7 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    19 white prepupae new    17 white prepupae 12hr    17 white prepupae 24hr    15 pupae 2d postWPP    11 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    11 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    9 embryo 02-04hr    11 embryo 04-06hr    14 embryo 06-08hr    13 embryo 08-10hr    14 embryo 10-12hr    7 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    7 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    19 white prepupae new    17 white prepupae 12hr    17 white prepupae 24hr    15 pupae 2d postWPP    11 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    11 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9 embryo 02-04hr    11 embryo 04-06hr    14 embryo 06-08hr    13 embryo 08-10hr    14 embryo 10-12hr    7 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    7 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    19 white prepupae new    17 white prepupae 12hr    17 white prepupae 24hr    15 pupae 2d postWPP    11 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    11 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9 embryo 02-04hr    11 embryo 04-06hr    14 embryo 06-08hr    13 embryo 08-10hr    14 embryo 10-12hr    7 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    7 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    19 white prepupae new    17 white prepupae 12hr    17 white prepupae 24hr    15 pupae 2d postWPP    11 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    11 adult female 30day    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    9 embryo 02-04hr    11 embryo 04-06hr    14 embryo 06-08hr    13 embryo 08-10hr    14 embryo 10-12hr    7 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    7 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    19 white prepupae new    17 white prepupae 12hr    17 white prepupae 24hr    15 pupae 2d postWPP    11 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    11 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    9 embryo 02-04hr    11 embryo 04-06hr    14 embryo 06-08hr    13 embryo 08-10hr    14 embryo 10-12hr    7 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    7 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    19 white prepupae new    17 white prepupae 12hr    17 white prepupae 24hr    15 pupae 2d postWPP    11 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    11 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9 embryo 02-04hr    11 embryo 04-06hr    14 embryo 06-08hr    13 embryo 08-10hr    14 embryo 10-12hr    7 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    7 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    19 white prepupae new    17 white prepupae 12hr    17 white prepupae 24hr    15 pupae 2d postWPP    11 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    11 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9 embryo 02-04hr    11 embryo 04-06hr    14 embryo 06-08hr    13 embryo 08-10hr    14 embryo 10-12hr    7 embryo 12-14hr    15 embryo 14-16hr    12 embryo 16-18hr    11 embryo 18-20hr    9 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    7 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    19 white prepupae new    17 white prepupae 12hr    17 white prepupae 24hr    15 pupae 2d postWPP    11 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    7 adult male 30day    7 adult female 01day    8 adult female 05day    11 adult female 30day    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    2 embryonic 1182-4H    8 embryonic GM2    9 embryonic Kc167    3 embryonic S1    3 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    3 wing disc ML-DmD8    9 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    1 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    8 ovary fGS/OSS    11 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    2 embryonic 1182-4H    8 embryonic GM2    9 embryonic Kc167    3 embryonic S1    3 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    3 wing disc ML-DmD8    9 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    1 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    8 ovary fGS/OSS    11 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    2 embryonic 1182-4H    8 embryonic GM2    9 embryonic Kc167    3 embryonic S1    3 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    3 wing disc ML-DmD8    9 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    1 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    8 ovary fGS/OSS    11 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    2 embryonic 1182-4H    8 embryonic GM2    9 embryonic Kc167    3 embryonic S1    3 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    3 wing disc ML-DmD8    9 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    1 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    8 ovary fGS/OSS    11 ovary OSC    8 ovary OSS    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    2 embryonic 1182-4H    8 embryonic GM2    9 embryonic Kc167    3 embryonic S1    3 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    3 wing disc ML-DmD8    9 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    1 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    8 ovary fGS/OSS    11 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    2 embryonic 1182-4H    8 embryonic GM2    9 embryonic Kc167    3 embryonic S1    3 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    3 wing disc ML-DmD8    9 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    1 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    8 ovary fGS/OSS    11 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    2 embryonic 1182-4H    8 embryonic GM2    9 embryonic Kc167    3 embryonic S1    3 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    3 wing disc ML-DmD8    9 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    1 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    8 ovary fGS/OSS    11 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    5 Schneider line 2 Sg4    2 embryonic 1182-4H    8 embryonic GM2    9 embryonic Kc167    3 embryonic S1    3 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    3 wing disc ML-DmD8    9 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    1 wing disc ML-DmD32    5 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    4 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    8 ovary fGS/OSS    11 ovary OSC    8 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0032621
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(2L)Exel8036) tweekc01084 lethal (with tweek2) tweekEY02585-Δ1 lethal (with tweekc01084) tweekEY02585-Δ1 lethal (with tweekEY02585-Δ1) tweek2 tweekc01084 lethal | first instar larval stage | recessive tweek1 lethal | pharate adult stage (with Df(2L)Exel8036) tweek1 tweek2 lethal | pharate adult stage (with tweek1) tweek2 tweekc01084 lethal | pharate adult stage (with tweek2) tweek1 tweekc01084 tweekEY02585-Δ1 lethal | pharate adult stage (with tweekc01084) tweek1 tweek2 lethal | pharate adult stage (with tweekEY02585-Δ1) tweek2 lethal | pharate adult stage | recessive tweek2 lethal | recessive | second instar larval stage tweek1 viable, with Scer\GAL4pnr-MD237 tweekGD8917 tweekGD11444 Other Phenotypes Allele neurophysiology defective (with Df(2L)Exel8036) tweek1 tweek2 neurophysiology defective (with tweek1) tweek2 neurophysiology defective (with tweek2) tweek1 tweekc01084 neurophysiology defective (with tweekc01084) tweek2 neurophysiology defective | somatic clone tweek1 tweek2 uncoordinated | adult stage tweek2 uncoordinated | adult stage (with Df(2L)Exel8036) tweek1 tweek2 uncoordinated | adult stage (with tweek1) tweek2 tweekc01084 uncoordinated | adult stage (with tweek2) tweek1 tweekc01084 tweekEY02585-Δ1 uncoordinated | adult stage (with tweekc01084) tweek1 tweek2 uncoordinated | adult stage (with tweekEY02585-Δ1) tweek2 Phenotype manifest in Allele embryonic/larval neuromuscular junction | somatic clone | third instar larval stage (with tweek1) tweek2 embryonic/larval neuromuscular junction | somatic clone | third instar larval stage (with tweek2) tweek1 optic cartridge | somatic clone tweek1 synaptic vesicle (with Df(2L)Exel8036) tweek2 synaptic vesicle | somatic clone tweek1
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of tweek Class Mutagen Stocks Known lesion tweek1 ethyl methanesulfonate 2 Yes tweekEY02585 2 -- tweek2 ethyl methanesulfonate 1 Yes tweekc01084 1 -- tweekf05507 piggyBac transposase 1 -- tweekEY02585-Δ1 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of tweek Class Mutagen Stocks Known lesion tweekGD11444 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes tweekGD8917 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes tweekKK103494 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tweek+tHB69 in vitro construct - genomic fragment 0 Yes tweekKK101146 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes tweekNIG.4841R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes tweektCH321-79N05 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel8036 (Ryder, 2004.4.26, Verstreken et al., 2009) Df(2L)EY02585Δ1 (Verstreken et al., 2009)
Partially disrupted in	Df(2L)EY02585Δ1 (Verstreken et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK101146} NA P{NIG.4841R} NA characterization construct P{tweek+tHB69} NA PBac{CH321-79N05} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}ApepPEY02585 NA miscellaneous insertions PBac{PB}tweekc01084 NA PBac{WH}tweekf05507 NA
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
	There is no information supporting a molecular function term for this gene. (Based on a review of available data on: 2009-02-19)
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
synaptic vesicle endocytosis inferred from mutant phenotype (Verstreken and Bellen, 2006, Verstreken et al., 2009)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cobalamin metabolic process inferred from electronic annotation with InterPro:IPR019362 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
mitochondrion inferred from electronic annotation with InterPro:IPR019362 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene (FlyBase Genome Annotators, 2008, ) protein_coding_gene (FlyBase Genome Annotators, 2008, )
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions tweek allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261671 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35026
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6Z8ZFR Dipteran inclusive ortholog search EOG63FH4T Insect inclusive ortholog search EOG6THTNV Arthropod inclusive ortholog search EOG6B8GTV Metazoa inclusive ortholog search EOG6BS8C8
Orthologs in Drosophila Species (EOG6Z8ZFR)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\tweek     Drosophila willistoni   Dwil\GK23792     Drosophila virilis   Dvir\GJ16182
Orthologs in non-Drosophila Dipterans (EOG63FH4T)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008836   Anopheles gambiae Malaria mosquito  Agam\AGAP007960   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001558
Orthologs in non-Dipteran Insects (EOG6THTNV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB52098   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG029264   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG025076   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10489   Atta cephalotes Leafcutter ant  Acep\ACEP23313   Camponotus floridanus Florida carpenter ant  Cflo\CFLO23063   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21097   Linepithema humile Argentine ant  Lhum\LH16800   Pogonomyrmex barbatus Red harvester ant  Pbar\PB14935   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12338   Solenopsis invicta Red fire ant  Sinv\SINV15128   Solenopsis invicta Red fire ant  Sinv\SINV15122   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006956   Bombyx mori Silkmoth  Bmor\BGIBMGA003784   Bombyx mori Silkmoth  Bmor\BGIBMGA003783   Pediculus humanus Human body louse  Phum\PHUM335460   Tribolium castaneum Red flour beetle  Tcas\GLEAN_15637
Orthologs in non-Insect Arthropods (EOG6B8GTV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_328986   Ixodes scapularis Deer tick  Isca\ISCW017338   Ixodes scapularis Deer tick  Isca\ISCW024023   Ixodes scapularis Deer tick  Isca\ISCW007766
Orthologs in non-Arthropod Metazoa (EOG6BS8C8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\Y47G6A.29   Caenorhabditis elegans Nematode  Cele\lpd-3   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001252gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006238gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008721gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007475gn   Danio rerio Zebrafish  Drer\si:ch211-233a24.2   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000001596   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000000994   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000004799   Xenopus tropicalis Western clawed frog  Xtro\kiaa1109   Gallus gallus Domestic chicken  Ggal\KIAA1109   Mus musculus House mouse  Mmus\Kiaa1109   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000032689   Rattus norvegicus Norway rat  Rnor\RGD1309985   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000038436   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000026660   Homo sapiens Human  Hsap\KIAA1109
Human Orthologs (1)
Gene OMIM HGNC Hsap\KIAA1109
AAA Orthologs (18) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24095 Drosophila simulans Dsim\GD24096 Drosophila sechellia Dsec\GM17220 Drosophila sechellia Dsec\GM17221 Drosophila erecta Dere\GG21070 Drosophila erecta Dere\GG21071 Drosophila yakuba Dyak\GE12779 Drosophila yakuba Dyak\GE12780 Drosophila ananassae Dana\GF14779 Drosophila ananassae Dana\GF14780 Drosophila pseudoobscura pseudoobscura Dpse\GA13520 Drosophila pseudoobscura pseudoobscura Dpse\GA18470 Drosophila persimilis Dper\GL14221 Drosophila persimilis Dper\GL14232 Drosophila mojavensis Dmoj\GI17140 Drosophila mojavensis Dmoj\GI17141 Drosophila grimshawi Dgri\GH11696 Drosophila grimshawi Dgri\GH11697
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	15423 y1 w67c23; P{EPgy2}ApepPEY02585/CyO 39695 w*; P{UAS-GFP-myc-2xFYVE}2, tweek1/T(2;3)TSTL, CyO: TM6B, Tb1 39696 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; tweek1 P{Car20y}25F P{neoFRT}40A/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7 39697 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; tweek2 P{Car20y}25F P{neoFRT}40A/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7
Harvard	- PBac{PB}tweekc01084 - PBac{WH}tweekf05507
Kyoto	111703 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{EPgy2}ApepPEY02585 P{neoFRT}40A/CyO y+
VDRC	v102639 P{KK103494}VIE-260B v26645 w1118; P{GD11444}v26645 v19305 w1118; P{GD8917}v19305/TM3 v19306 w1118; P{GD8917}v19306/TM3 v26646 w1118; P{GD11444}v26646/CyO
Genomic Clones ( 1 )
	BACR27M18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 27 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT17578 AT18168 HL01455 RH03839
Other clones	7650 59538 74949 214283.31 214415.30 214573.20 CK01.121B.C11 Dmel_neb_013462_0951_1204 DMG4-chr2L.28.012.pred.a EC28689 EK092810 EK125541 EK184347 EK206048 EP10728 EP14333 EP15627 EP16116 EP19913 GH27425 MB6-CG15134-RA RH09427 RP003066665
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261671 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35026
Antibody Information
Other Information
Discoverer
Etymology
	Named after a common street name for methamphetamine, because mutant escaper flies are severely uncoordinated. (Verstreken and Bellen, 2006) The gene is named "tweek" as the behaviour of rare mutant adult escapers is reminiscent of the cartoon character "Tweek" from the TV series "South Park". (Verstreken et al., 2009)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: tweek CG42555 CG15134 CG4841 (Verstreken et al., 2009)
Additional comments	Annotations CG42555, CG15134 and CG4841 merged as CG42706 in release 5.26 of the genome annotation. (FlyBase Genome Annotators, 2010) Annotation CG15133 split into CG42555 and CG42556 in release 5.16 of the genome annotation. (FlyBase Genome Annotators, 2008)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001201895 NM_001201896 NM_001201897 NM_001201898 RefSeq (Proteins) • NP_001188824 NP_001188825 NP_001188826 NP_001188827 DDBJ / EMBL / GenBank • AAF53586 AAF53588 AAF53590 AAM03127 ACL83037 ADV37073 ADV37074 ADV37075 ADV37076 AF489106 AI516845 AW941059 BF501979 BF502442 BI573991 BI590247 CO342855 CZ488099 EC208903 EC220325 EC256100 FL502728 FL502729 Entrez Gene - A searchable database of RefSeq genes. • 35026 35028 35030 UniProtKB/TrEMBL • B7YZW5 Q8T620 Q9VJF9 Q9VJG1 Q9VJG3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Methylmalonic aciduria and homocystinuria type D protein (IPR019362) Fragile site-associated protein, C-terminal (IPR018863)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261671 DRSC - Results from RNAi screens. • FBgn0261671 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0032621 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261671 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35026 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35026 modMine - Data generated by the modENCODE project. • FBgn0261671
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG4841 (Ellis and Carney, 2009, Verstreken et al., 2009, Maisonhaute et al., 2007, Japanese National Institute of Genetics, 2012.5.21) CG15133 (Verstreken et al., 2009, Keleman et al., 2009.8.5) CG15134 (Verstreken et al., 2009, Keleman et al., 2009.8.5) CG42555 (FlyBase Genome Annotators, 2008, ) CG42706 (FlyBase Genome Annotators, 2010, ) tweek (Verstreken et al., 2009, Venken et al., 2009, Wasbrough et al., 2010) Tweek (Khuong et al., 2010)
Name Synonym	tweek (Habets et al., 2008)
Secondary FlyBase IDs
	FBan0015133 FBan0015134 FBgn0032619 FBgn0032621 FBgn0032622 FBgn0084472 FBgn0260757 FBgn0261623
References ( 24 )
Generate a list of	All references relating to this gene ( 24 )
List References by type	Research paper  ( 6 ) Supplementary material  ( 3 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 2 ) FlyBase analysis  ( 5 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...