Gene Dmel\Npc1b

FlyGene Wiki Help Close All Open All

General Information

Symbol

Dmel\Npc1b

Species

D. melanogaster

Name

Niemann-Pick type C-1b

Annotation symbol

CG12092

Feature type

protein_coding_gene

FlyBase ID

FBgn0261675

Gene Model Status

Current

Stock availability

6 publicly available

Also Known As

R-9-28, NPC1b, 151, l(1)R-9-28, l(1)19Ec

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

19E5-19E5

Sequence location

X:20,805,980..20,810,408 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Niemann-Pick type C-1b is referred to in FlyBase by the symbol Dmel\Npc1b (CG12092, FBgn0261675). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: hedgehog receptor activity. There is experimental evidence that it is involved in the biological process: central nervous system development; dorsal closure; intestinal cholesterol absorption; peripheral nervous system development. 37 alleles are reported. The phenotypes of these alleles are annotated with: dorsal closure embryo; embryonic/first instar larval cuticle; embryonic head; trichogen cell. It has one annotated transcript and one annotated polypeptide. Protein features are: Patched; Sterol-sensing domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of no expression detected. Peak expression observed during early larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult midgut. Expression at moderate levels in the following post-embryonic organs or tissues: larval midgut. Comments on Affy2 ProbeSet: ProbeSet 1632659_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Npc1b. Gene sequence location is X:20805980..20810408.

User Contributed Data

Beta version, feedback welcome

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

l(1)19Ec

Mutant embryos from heterozygous females exhibit no defects in central or peripheral nervous systems; l(1)19Ec1 and l(1)19Ec18 are lethal in germ-line clones, whereas l(1)19Ec8 and l(1)19Ec25 germ-line clones exhibit a paternally rescuable maternal effect. Embryonic phenotype includes head and dorsal-closure defects, and most embryos are twisted (Perrimon, Smouse, and Miklos, 1989, Genetics 121: 313-31).

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

Update Feed

Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.

FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

19E5-19E5

Limits computationally determined from genome sequence between P{EP}CG1702^EP1525 and P{EP}EP1465&P{EP}CG1486^EP1192

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

19E4-19E4

Determined by complementation mapping, aberrations not specified.

(Perrimon et al., 1989)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Npc1b for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Npc1b-RA

FBtr0077239

NM_134573

3765

1254

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Npc1b-PA

FBpp0076932

139.6

1254

4.50

NP_608417

AAF50873

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Sterol-sensing domain (IPR000731)

Patched (IPR003392)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VRC9

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult midgut. Expression at moderate levels in the following post-embryonic organs or tissues: larval midgut.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1.475
Larval Midgut		487.8
Larval Hindgut		3.9
Larval Malpighian Tubules		1
Larval Fat Body		0.7
Larval Salivary Gland		2.1
Larval Trachea		2.575
Larval Carcass		2.5
Adult Head		1.1
Adult Eye		2.25
Adult Brain		2.5
Adult Thoracic-Abdominal Ganglion		5.7
Adult Crop		3.9
Adult Midgut		765.8
Adult Hindgut		5
Adult Malpighian Tubules		3.6
Adult Fat Body		5.9
Adult Salivary Gland		8.7
Adult Heart		2.7
Adult VirginFemale Spermatheca		4.2
Adult InseminatedFemale Spermatheca		5.4
Adult Ovary		0.9
Adult Testis		3.4
Adult Male Accessory Gland		2.5
Adult Carcass		10.8
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		1.475
Larval Midgut		487.8
Larval Hindgut		3.9
Larval Malpighian Tubules		1
Larval Fat Body		0.7
Larval Salivary Gland		2.1
Larval Trachea		2.575
Larval Carcass		2.5
Adult Head		1.1
Adult Eye		2.25
Adult Brain		2.5
Adult Thoracic-Abdominal Ganglion		5.7
Adult Crop		3.9
Adult Midgut		(765.8)
Adult Hindgut		5
Adult Malpighian Tubules		3.6
Adult Fat Body		5.9
Adult Salivary Gland		8.7
Adult Heart		2.7
Adult VirginFemale Spermatheca		4.2
Adult InseminatedFemale Spermatheca		5.4
Adult Ovary		0.9
Adult Testis		3.4
Adult Male Accessory Gland		2.5
Adult Carcass		10.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1.475
Larval Midgut		487.8
Larval Hindgut		3.9
Larval Malpighian Tubules		1
Larval Fat Body		0.7
Larval Salivary Gland		2.1
Larval Trachea		2.575
Larval Carcass		2.5
Adult Head		1.1
Adult Eye		2.25
Adult Brain		2.5
Adult Thoracic-Abdominal Ganglion		5.7
Adult Crop		3.9
Adult Midgut		765.8
Adult Hindgut		5
Adult Malpighian Tubules		3.6
Adult Fat Body		5.9
Adult Salivary Gland		8.7
Adult Heart		2.7
Adult VirginFemale Spermatheca		4.2
Adult InseminatedFemale Spermatheca		5.4
Adult Ovary		0.9
Adult Testis		3.4
Adult Male Accessory Gland		2.5
Adult Carcass		10.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1.475
Larval Midgut		487.8
Larval Hindgut		3.9
Larval Malpighian Tubules		1
Larval Fat Body		0.7
Larval Salivary Gland		2.1
Larval Trachea		2.575
Larval Carcass		2.5
Adult Head		1.1
Adult Eye		2.25
Adult Brain		2.5
Adult Thoracic-Abdominal Ganglion		5.7
Adult Crop		3.9
Adult Midgut		765.8
Adult Hindgut		5
Adult Malpighian Tubules		3.6
Adult Fat Body		5.9
Adult Salivary Gland		8.7
Adult Heart		2.7
Adult VirginFemale Spermatheca		4.2
Adult InseminatedFemale Spermatheca		5.4
Adult Ovary		0.9
Adult Testis		3.4
Adult Male Accessory Gland		2.5
Adult Carcass		10.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1.475
Larval Midgut		487.8
Larval Hindgut		3.9
Larval Malpighian Tubules		1
Larval Fat Body		0.7
Larval Salivary Gland		2.1
Larval Trachea		2.575
Larval Carcass		2.5
Adult Head		1.1
Adult Eye		2.25
Adult Brain		2.5
Adult Thoracic-Abdominal Ganglion		5.7
Adult Crop		3.9
Adult Midgut		765.8
Adult Hindgut		5
Adult Malpighian Tubules		3.6
Adult Fat Body		5.9
Adult Salivary Gland		8.7
Adult Heart		2.7
Adult VirginFemale Spermatheca		4.2
Adult InseminatedFemale Spermatheca		5.4
Adult Ovary		0.9
Adult Testis		3.4
Adult Male Accessory Gland		2.5
Adult Carcass		10.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		1.475
Larval Midgut		487.8
Larval Hindgut		3.9
Larval Malpighian Tubules		1
Larval Fat Body		0.7
Larval Salivary Gland		2.1
Larval Trachea		2.575
Larval Carcass		2.5
Adult Head		1.1
Adult Eye		2.25
Adult Brain		2.5
Adult Thoracic-Abdominal Ganglion		5.7
Adult Crop		3.9
Adult Midgut		765.8
Adult Hindgut		5
Adult Malpighian Tubules		3.6
Adult Fat Body		5.9
Adult Salivary Gland		8.7
Adult Heart		2.7
Adult VirginFemale Spermatheca		4.2
Adult InseminatedFemale Spermatheca		5.4
Adult Ovary		0.9
Adult Testis		3.4
Adult Male Accessory Gland		2.5
Adult Carcass		10.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1.475
Larval Midgut		487.8
Larval Hindgut		3.9
Larval Malpighian Tubules		1
Larval Fat Body		0.7
Larval Salivary Gland		2.1
Larval Trachea		2.575
Larval Carcass		2.5
Adult Head		1.1
Adult Eye		2.25
Adult Brain		2.5
Adult Thoracic-Abdominal Ganglion		5.7
Adult Crop		3.9
Adult Midgut		765.8
Adult Hindgut		5
Adult Malpighian Tubules		3.6
Adult Fat Body		5.9
Adult Salivary Gland		8.7
Adult Heart		2.7
Adult VirginFemale Spermatheca		4.2
Adult InseminatedFemale Spermatheca		5.4
Adult Ovary		0.9
Adult Testis		3.4
Adult Male Accessory Gland		2.5
Adult Carcass		10.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1.475
Larval Midgut		487.8
Larval Hindgut		3.9
Larval Malpighian Tubules		1
Larval Fat Body		0.7
Larval Salivary Gland		2.1
Larval Trachea		2.575
Larval Carcass		2.5
Adult Head		1.1
Adult Eye		2.25
Adult Brain		2.5
Adult Thoracic-Abdominal Ganglion		5.7
Adult Crop		3.9
Adult Midgut		765.8
Adult Hindgut		5
Adult Malpighian Tubules		3.6
Adult Fat Body		5.9
Adult Salivary Gland		8.7
Adult Heart		2.7
Adult VirginFemale Spermatheca		4.2
Adult InseminatedFemale Spermatheca		5.4
Adult Ovary		0.9
Adult Testis		3.4
Adult Male Accessory Gland		2.5
Adult Carcass		10.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	1.1
NA	Eye	2.25
NA	Brain	2.5
1.475	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	5.7
NA	Crop	3.9
487.8	Midgut	765.8
3.9	Hindgut	5
1	Malpighian Tubules	3.6
0.7	Fat Body	5.9
2.1	Salivary Gland	8.7
NA	Heart	2.7
2.575	Trachea	NA
NA	VirginFemale Spermatheca	4.2
NA	InseminatedFemale Spermatheca	5.4
NA	Ovary	0.9
NA	Testis	3.4
NA	Male Accessory Gland	2.5
2.5	Carcass	no informative data

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		41
digestive system, 4-day adult		37
digestive system, 20-day adult		43
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		(41)
digestive system, 4-day adult		(37)
digestive system, 20-day adult		(43)
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		41
digestive system, 4-day adult		37
digestive system, 20-day adult		43
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		41
digestive system, 4-day adult		37
digestive system, 20-day adult		43
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		41
digestive system, 4-day adult		37
digestive system, 20-day adult		43
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		(41)
digestive system, 4-day adult		(37)
digestive system, 20-day adult		(43)
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		41
digestive system, 4-day adult		37
digestive system, 20-day adult		43
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		0
central nervous system, pupae P8		0
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		0
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		41
digestive system, 4-day adult		37
digestive system, 20-day adult		43
fat body, larvae L3 wandering		0
fat body, white prepupae		0
fat body, pupae P8		0
carcass, larvae L3 wandering		0
carcass, 1-day adult		0
carcass, 4-day adult		0
carcass, 20-day adult		0
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		0
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of no expression detected. Peak expression observed during early larval stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		3
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		15
larva L1		15
larva L2		22
larva L3 12hr old		6
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		4
larva L3 puffstage 7-9		6
white prepupae new		4
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		0
pupae 3d postWPP		0
pupae 4d postWPP		0
adult male 01day		3
adult male 05day		4
adult male 30day		3
adult female 01day		10
adult female 05day		13
adult female 30day		6
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		3
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		15
larva L1		15
larva L2		22
larva L3 12hr old		6
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		4
larva L3 puffstage 7-9		6
white prepupae new		4
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		0
pupae 3d postWPP		0
pupae 4d postWPP		0
adult male 01day		3
adult male 05day		4
adult male 30day		3
adult female 01day		10
adult female 05day		13
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		3
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		15
larva L1		15
larva L2		22
larva L3 12hr old		6
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		4
larva L3 puffstage 7-9		6
white prepupae new		4
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		0
pupae 3d postWPP		0
pupae 4d postWPP		0
adult male 01day		3
adult male 05day		4
adult male 30day		3
adult female 01day		10
adult female 05day		13
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		3
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		15
larva L1		15
larva L2		22
larva L3 12hr old		6
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		4
larva L3 puffstage 7-9		6
white prepupae new		4
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		0
pupae 3d postWPP		0
pupae 4d postWPP		0
adult male 01day		3
adult male 05day		4
adult male 30day		3
adult female 01day		10
adult female 05day		13
adult female 30day		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		3
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		15
larva L1		15
larva L2		22
larva L3 12hr old		6
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		4
larva L3 puffstage 7-9		6
white prepupae new		4
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		0
pupae 3d postWPP		0
pupae 4d postWPP		0
adult male 01day		3
adult male 05day		4
adult male 30day		3
adult female 01day		10
adult female 05day		13
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		3
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		15
larva L1		15
larva L2		22
larva L3 12hr old		6
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		4
larva L3 puffstage 7-9		6
white prepupae new		4
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		0
pupae 3d postWPP		0
pupae 4d postWPP		0
adult male 01day		3
adult male 05day		4
adult male 30day		3
adult female 01day		10
adult female 05day		13
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		3
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		15
larva L1		15
larva L2		22
larva L3 12hr old		6
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		4
larva L3 puffstage 7-9		6
white prepupae new		4
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		0
pupae 3d postWPP		0
pupae 4d postWPP		0
adult male 01day		3
adult male 05day		4
adult male 30day		3
adult female 01day		10
adult female 05day		13
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		0
embryo 08-10hr		0
embryo 10-12hr		0
embryo 12-14hr		0
embryo 14-16hr		3
embryo 16-18hr		7
embryo 18-20hr		10
embryo 20-22hr		17
embryo 22-24hr		15
larva L1		15
larva L2		22
larva L3 12hr old		6
larva L3 puffstage 1-2		8
larva L3 puffstage 3-6		4
larva L3 puffstage 7-9		6
white prepupae new		4
white prepupae 12hr		0
white prepupae 24hr		0
pupae 2d postWPP		0
pupae 3d postWPP		0
pupae 4d postWPP		0
adult male 01day		3
adult male 05day		4
adult male 30day		3
adult female 01day		10
adult female 05day		13
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		2
cold shock, 4-day adult		1
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		3
Cadmium 50 mM 48 hrs, 4-day adult		7
Cadmium 100 mM 48 hrs, 4-day adult		0
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		1
Ethanol 5% 3 hrs, larvae L3		14
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		6
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0031140

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

cell lethal | germline clone

lethal

Npc1b^R9-28

lethal | germline clone | recessive | rescuable maternal effect

Npc1b^LB2

lethal | germline clone | rescuable maternal effect

Npc1b^R9-28

lethal | larval stage

lethal | larval stage | recessive

lethal | partially | rescuable maternal effect

lethal | pupal stage | recessive

Npc1b^R9-28

lethal | recessive

lethal | recessive | rescuable maternal effect

Npc1b^LB2

lethal | recessive | second instar larval stage

Npc1b¹

lethal | second instar larval stage (with Npc1b¹)

Npc1b^R9-28

lethal | second instar larval stage (with Npc1b^R9-28)

Npc1b¹

viable, with Scer\GAL4^pnr-MD237

Npc1b^GD12644

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

Npc1b^GD12644

Phenotype manifest in

Allele

dorsal closure embryo

embryo | germline clone

Npc1b^R9-28

embryonic/first instar larval cuticle

Npc1b¹⁵¹

embryonic/first instar larval cuticle | germline clone

Npc1b^R9-28

embryonic head

trichogen cell, with Scer\GAL4^pnr-MD237

Npc1b^GD12644

Classical Alleles ( 29 )

For All Classical Alleles Show

Allele of Npc1b	Class	Mutagen	Stocks	Known lesion
Npc1b^LB2		ethyl methanesulfonate	1	--
Npc1b^R9-28		ethyl methanesulfonate	1	Yes
Npc1b¹	amorphic allele - genetic evidence	gene targeting by homologous recombination	0	Yes
Npc1b¹⁵¹		X ray	0	--
Npc1b^17-344		neutrons	0	--
Npc1b^17-457			0	--
Npc1b^4-2		spontaneous	0	--
Npc1b^AF2-19		methyl methanesulfonate	0	--
Npc1b^DA507		ethyl methanesulfonate	0	--
Npc1b^DA536		ethyl methanesulfonate	0	--
Npc1b^DCB2-35a			0	--
Npc1b^EC242		ethyl methanesulfonate	0	--
Npc1b^GA71		X ray	0	--
Npc1b^HC279		X ray	0	--
Npc1b^HF417		X ray	0	--
Npc1b^J28		X ray	0	--
Npc1b^J2			0	--
Npc1b^J9			0	--
Npc1b^KA12		X ray	0	--
Npc1b^L39		X ray	0	--
Npc1b^N5		spontaneous	0	--
Npc1b^P464		ethyl methanesulfonate	0	--
Npc1b^Q256		ethyl methanesulfonate	0	--
Npc1b^R-9-2		ethyl methanesulfonate	0	--
Npc1b^t2-14a			0	--
Npc1b^VE863		ethyl methanesulfonate	0	--
Npc1b^VE866		ethyl methanesulfonate	0	--
Npc1b^VE926		ethyl methanesulfonate	0	--
Npc1b^YT6		ethyl methanesulfonate	0	--

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Npc1b	Mutagen	Stocks	Known lesion
Npc1b^GD12644	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Npc1b^HMS01757	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Npc1b^KK106937	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Npc1b^+t6.6	in vitro construct - genomic fragment	0	Yes
Npc1b^NIG.11233R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Npc1b^NIG.12092R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Npc1b^{Scer\SceI.RS.cVa}	in vitro construct	0	Yes
Npc1b^{Scer\UAS.P\T.cVa}	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(1)16-1-88

(Munoz, 2004.1.5)

Df(1)16-3-22

(Torres and Sanchez, 1992, Miklos et al., 1987)

Df(1)16-3-35

(Paradi et al., 1983, Kramers et al., 1983)

Df(1)16-3-95

(Munoz, 2004.1.5)

Df(1)16-3-129

(Munoz, 2004.1.5)

Df(1)16-3-160

(Munoz, 2004.1.5)

Df(1)17-322

(Munoz, 2004.1.5)

Df(1)17-351

(Miklos et al., 1987, Miklos and de Couet, 1990, Perrimon et al., 1989)

Df(1)18-36

(Munoz, 2004.1.5)

Df(1)19-18

(Munoz, 2003.12.10, Munoz, 2004.1.5)

Df(1)26B

(Perrimon et al., 1989, Zusman et al., 1985)

Df(1)217

(Paradi et al., 1983)

Df(1)A118

(Voght et al., 2007, Torres and Sanchez, 1992, Paradi et al., 1983, Kramers et al., 1991, Lifschytz and Yakobovitz, 1978, Balakrishnan and Rodrigues, 1991, Miklos et al., 1987, Lifschytz and Falk, 1969, Kramers et al., 1983, Perrimon et al., 1989)

Df(1)B12

(Lifschytz and Yakobovitz, 1978, Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)B57

(Miklos et al., 1987, Perrimon et al., 1989, Torres and Sanchez, 1992, Lifschytz and Yakobovitz, 1978, Miklos and de Couet, 1990, Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)C1

(Voght et al., 2007)

Df(1)GA37

(Perrimon et al., 1989)

Df(1)GA40

(Perrimon et al., 1989)

Df(1)HC279

(Perrimon et al., 1989)

Df(1)HM44

(Miklos et al., 1987, Miklos et al., 1986, Miklos and de Couet, 1990, Perrimon et al., 1989, Kramers et al., 1983)

Df(1)HM45

(Kramers et al., 1983)

Df(1)LB6

(Torres and Sanchez, 1992, Perrimon et al., 1989)

Df(1)LB7

(Perrimon et al., 1989)

Df(1)mal3

(Kramers et al., 1983, Kramers et al., 1991)

Df(1)mal5

(Schalet and Finnerty, 1968)

Df(1)mal6

(Kramers et al., 1983, Kramers et al., 1991, Paradi et al., 1983, Schalet and Finnerty, 1968)

Df(1)mal8

(Schalet and Finnerty, 1968, Schalet and Finnerty, 1968)

Df(1)mal10

(Schalet and Finnerty, 1968)

Df(1)mal12

(Zusman et al., 1985, Paradi et al., 1983)

Df(1)mal13

(Schalet and Finnerty, 1968)

Df(1)N77

(Perrimon et al., 1989)

Df(1)Q219

(Lifschytz and Falk, 1969)

Df(1)Q408

(Lifschytz and Falk, 1969)

Df(1)R-9-1

(Lifschytz and Falk, 1969)

Df(1)R-10-5

(Lifschytz and Falk, 1969)

Df(1)run1112

(Zusman et al., 1985, Torres and Sanchez, 1992, Perrimon et al., 1989)

Dp(1;Y)y⁺mal¹¹³

Not disrupted in

Df(1)2/19B

(Perrimon et al., 1989, Miklos and de Couet, 1990)

Df(1)16-2-13

(Miklos et al., 1987)

Df(1)16-2-14

(Munoz, 2004.1.5)

Df(1)16-3-35

(Perrimon et al., 1989, Miklos et al., 1987, Torres and Sanchez, 1992, Kramers et al., 1991, Munoz, 2004.1.5)

Df(1)16-3-53

(Munoz, 2004.1.5)

Df(1)16-129

(Miklos and de Couet, 1990, Miklos et al., 1987, Perrimon et al., 1989)

Df(1)17-25

(Munoz, 2004.1.5)

Df(1)17-137

(Kramers et al., 1991, Munoz, 2004.1.5)

Df(1)17-257

(Miklos and de Couet, 1990, Miklos et al., 1987, Perrimon et al., 1989)

Df(1)17-456

(Munoz, 2004.1.5)

Df(1)17-489

(Perrimon et al., 1989, Miklos et al., 1987)

Df(1)18-80

(Munoz, 2004.1.5)

Df(1)19-2

(Munoz, 2004.1.5)

Df(1)208

(Paradi et al., 1983)

Df(1)216

(Paradi et al., 1983)

Df(1)218

(Paradi et al., 1983)

Df(1)228

(Paradi et al., 1983)

Df(1)A19

(Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)A33

(Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)A53

(Torres and Sanchez, 1992, Perrimon et al., 1989)

Df(1)A58

(Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)A122

(Lifschytz and Falk, 1969, Lifschytz and Falk, 1968)

Df(1)A129

(Lifschytz and Falk, 1968)

Df(1)A161

(Lifschytz and Falk, 1968)

Df(1)A200

(Lifschytz and Falk, 1968)

Df(1)A209

(Lifschytz and Falk, 1968, Zusman et al., 1985, Lifschytz and Falk, 1969)

Df(1)A211

(Lifschytz and Falk, 1968)

Df(1)B18

(Lifschytz and Falk, 1968)

Df(1)B47

(Lifschytz and Falk, 1968)

Df(1)B56

(Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)B82

(Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)B85

(Lifschytz and Falk, 1968)

Df(1)B99

(Lifschytz and Falk, 1968)

Df(1)B111

(Munoz, 2004.1.5, Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)B155

(Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)B179

(Lifschytz and Falk, 1968)

Df(1)B182

(Lifschytz and Falk, 1968)

Df(1)B199

(Lifschytz and Falk, 1968)

Df(1)B214

(Lifschytz and Falk, 1969, Lifschytz and Falk, 1968)

Df(1)B225

(Lifschytz and Falk, 1968)

Df(1)B240

(Lifschytz and Falk, 1968)

Df(1)B255

(Lifschytz and Falk, 1968)

Df(1)B264

(Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)C74

(Perrimon et al., 1989, Miklos and de Couet, 1990)

Df(1)D43L1

(Schalet and Finnerty, 1968, Kramers et al., 1991, Paradi et al., 1983, Perrimon et al., 1989, Kramers et al., 1983)

Df(1)DCB1-35b

(Zusman et al., 1985, Perrimon et al., 1989)

Df(1)DCB1-35c

(Miklos et al., 1987, Schalet and Finnerty, 1968)

Df(1)EA113

(Miklos et al., 1987)

Df(1)GA22

(Miklos et al., 1987)

Df(1)GA33

(Perrimon et al., 1989)

Df(1)GA90

(Zusman et al., 1985)

Df(1)GA104

(Perrimon et al., 1989, Miklos et al., 1987, Miklos and de Couet, 1990)

Df(1)GA131

(Miklos et al., 1987)

Df(1)GE263

(Miklos et al., 1987, Perrimon et al., 1989, Miklos and de Couet, 1990)

Df(1)HF359

(Miklos et al., 1987)

Df(1)HM49

(Kramers et al., 1983)

Df(1)HM50

(Kramers et al., 1983)

Df(1)JA21

(Miklos et al., 1987)

Df(1)JA117

(Perrimon et al., 1989, Miklos et al., 1987, Miklos and de Couet, 1990)

Df(1)JC4

(Miklos and de Couet, 1990, Miklos et al., 1987)

Df(1)JC77

(Miklos et al., 1987, Perrimon et al., 1989, Miklos and de Couet, 1990)

Df(1)LB23

(Perrimon et al., 1989)

Df(1)M31

(Lifschytz and Yakobovitz, 1978)

Df(1)M158

(Lifschytz and Yakobovitz, 1978)

Df(1)M171

(Lifschytz and Yakobovitz, 1978)

Df(1)M176

(Lifschytz and Yakobovitz, 1978)

Df(1)mal8

(Kramers et al., 1983, Kramers et al., 1991, Paradi et al., 1983, Zusman et al., 1985)

Df(1)mal10

(Perrimon et al., 1989, Paradi et al., 1983)

Df(1)mal17

(Perrimon et al., 1989)

Df(1)Q38

(Lifschytz and Falk, 1969)

Df(1)Q539

(Kramers et al., 1983, Lifschytz and Falk, 1969, Kramers et al., 1991, Zusman et al., 1985, Miklos et al., 1987, Miklos and de Couet, 1990, Perrimon et al., 1989, Paradi et al., 1983)

Df(1)S54

(Perrimon et al., 1989, Miklos and de Couet, 1990, Miklos et al., 1987)

Df(1)Sp20A-F

(Schalet, 1986)

Df(1)su(f)4B

(Kramers et al., 1991)

Df(1)su(f)724

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)733

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)754

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)795

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)7009

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)7085

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)9122

(Lifschytz and Yakobovitz, 1978)

Df(1)T2-14A

(Perrimon et al., 1989, Schalet and Lefevre, 1973, Munoz, 2004.1.5, Miklos et al., 1987)

Df(1)wl-2

(Lifschytz and Falk, 1969)

Df(1)X-1

(Lifschytz and Falk, 1968, Lifschytz and Falk, 1969)

Df(1)yl-1

(Lifschytz and Falk, 1969)

Df(1)y-X15

(Kramers et al., 1991)

Dp(1;Y)y⁺mal¹²⁶

(Lifschytz and Yakobovitz, 1978)

Not duplicated in

Dp(1;f)mini77

(Miklos and de Couet, 1990, Perrimon et al., 1989)

Dp(1;Y)B101

(Perrimon et al., 1989)

Dp(1;Y)B^S

(Kramers et al., 1991)

Dp(1;Y)y⁺mal¹²⁶

(Kramers et al., 1991, Miklos and de Couet, 1990)

Duplicated in

Dp(1;f)3

(Schalet and Finnerty, 1968)

Dp(1;f)52

(Schalet and Finnerty, 1968)

Dp(1;f)mini2

(Perrimon et al., 1989)

Dp(1;f)mini-ring

(Perrimon et al., 1989)

Dp(1;Y)mal⁺

(Lifschytz and Falk, 1968)

Dp(1;Y)y⁺mal¹⁰²

(Perrimon et al., 1989, Schalet and Finnerty, 1968)

Dp(1;Y)y⁺mal¹⁰⁶

(Miklos and de Couet, 1990)

Dp(1;Y)y⁺mal¹¹³

(Schalet and Finnerty, 1968)

Dp(1;Y)y⁺mal¹⁷¹

(Perrimon et al., 1989)

Dp(1;Y)y⁺mal⁺

(Lefevre, 1981)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

GAL4 construct

characterization construct

P{FRT(Npc1b^{Scer\SceI.RS.cVa})}

P{Npc1b^+t6.6}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )

Terms Based on Experimental Evidence ( 4 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

central nervous system development

inferred from mutant phenotype

(Perrimon et al., 1989)

dorsal closure

inferred from mutant phenotype

(Perrimon et al., 1989)

intestinal cholesterol absorption

inferred from mutant phenotype

(Voght et al., 2007)

peripheral nervous system development

inferred from mutant phenotype

(Perrimon et al., 1989)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function

CV term

Evidence

References

hedgehog receptor activity

inferred from electronic annotation with InterPro:IPR003392

(FlyBase Curators et al., 2004-)

Biological Process ( 0 terms)

Cellular Component

CV term

Evidence

References

integral to membrane

inferred from electronic annotation with InterPro:IPR000731

(FlyBase Curators et al., 2004-)

plasma membrane

inferred from sequence or structural similarity with EMBL:AF003348

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Npc1b allele

Gene

References

unspecified

cact

(Bhattacharya and Steward, 2003)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0261675

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

33072

Orthologs

OrthoDB Orthologs (57) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG669RD3

Dipteran inclusive ortholog search

EOG62Z4Z4

Insect inclusive ortholog search

EOG6CVF2S

Arthropod inclusive ortholog search

EOG67PVMT

Metazoa inclusive ortholog search

EOG6JMMGJ

Orthologs in Drosophila Species (EOG669RD3)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA11389

Drosophila persimilis

Dper\GL15811

Drosophila willistoni

Dwil\GK19822

Drosophila virilis

Dvir\GJ19256

Drosophila mojavensis

Dmoj\GI15499

Drosophila grimshawi

Dgri\GH17745

Orthologs in non-Drosophila Dipterans (EOG62Z4Z4)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009531

Anopheles gambiae

Malaria mosquito

Agam\AGAP000579

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ014970

Orthologs in non-Dipteran Insects (EOG6CVF2S)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB43203

Apis mellifera

Western honey bee

Amel\LOC408992

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013236

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012199

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH15012

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH23515

Atta cephalotes

Leafcutter ant

Acep\ACEP17551

Atta cephalotes

Leafcutter ant

Acep\ACEP25550

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17327

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO18737

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL13494

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL20567

Linepithema humile

Argentine ant

Lhum\LH23787

Linepithema humile

Argentine ant

Lhum\LH16950

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB22231

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB13463

Solenopsis invicta

Red fire ant

Sinv\SINV23509

Solenopsis invicta

Red fire ant

Sinv\SINV25654

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009020

Bombyx mori

Silkmoth

Bmor\BGIBMGA009049

Pediculus humanus

Human body louse

Phum\PHUM472470

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_05088

Orthologs in non-Insect Arthropods (EOG67PVMT)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_328579

Daphnia pulex

Water flea

Dpux\JGI_V11_321920

Ixodes scapularis

Deer tick

Isca\ISCW020300

Ixodes scapularis

Deer tick

Isca\ISCW006533

Orthologs in non-Arthropod Metazoa (EOG6JMMGJ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\ncr-1

Caenorhabditis elegans

Nematode

Cele\ncr-2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008300gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_009669gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016084gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_017452gn

Danio rerio

Zebrafish

Drer\ENSDARG00000077891

Danio rerio

Zebrafish

Drer\npc1

Xenopus tropicalis

Western clawed frog

Xtro\npc1

Xenopus tropicalis

Western clawed frog

Xtro\npc1l1

Gallus gallus

Domestic chicken

Ggal\NPC1

Mus musculus

House mouse

Mmus\Npc1

Mus musculus

House mouse

Mmus\ENSMUSG00000020447

Rattus norvegicus

Norway rat

Rnor\Cdig2

Homo sapiens

Human

Hsap\NPC1L1

Homo sapiens

Human

Hsap\NPC1

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\NPC1L1

Hsap\NPC1

AAA Orthologs (20) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10854

Drosophila pseudoobscura pseudoobscura

Dpse\GA11389

Drosophila persimilis

Dper\GL15811

Drosophila willistoni

Dwil\GK19822

Drosophila willistoni

Dwil\GK20046

Drosophila virilis

Dvir\GJ18910

Drosophila virilis

Dvir\GJ19256

Drosophila mojavensis

Dmoj\GI15102

Drosophila mojavensis

Dmoj\GI15499

Drosophila grimshawi

Dgri\GH17745

Drosophila grimshawi

Dgri\GH17766

Stocks & Reagents

Stocks Listed in FlyBase ( 6 )

Bloomington

38296

y¹ v¹; P{TRiP.HMS01757}attP40

5739

w¹ Npc1b^R9-28/FM6/Dp(1;Y)mal⁺

Kyoto

101098

FM6, y^31d sc⁸ dm¹ B¹ / Npc1b^LB2 / Dp(1;Y)y⁺mal¹⁰⁶

VDRC

v108054

P{KK106937}VIE-260B

v35514

w¹¹¹⁸; P{GD12644}v35514

v35516

w¹¹¹⁸; P{GD12644}v35516

Genomic Clones ( 1 )

BACR11M14

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 2 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0261675

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

33072

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: CG12092 NPC1b

(Voght et al., 2003)

Source for database merge of

Source for merge of: Npc1b l(1)19Ec

(Voght et al., 2007)

Additional comments

The "l(1)19Ec" complementation group was previously associated with the CG11233 annotation in FlyBase, based on information in FBrf0155430. However, data from FBrf0193836 suggests that the "l(1)19Ec" complementation group in fact corresponds to the "CG12092" annotation. Thus, the CG11233 annotation has been split out into a separate gene report in FlyBase and the l(1)19Ec complementation group has instead been merged with the gene corresponding to the "CG12092" annotation (Npc1b). The CG11233 annotation has also been renamed to CG42707 in release 5.26 of the genome annotation to avoid confusion.

(FlyBase Genome Annotators, 2010)

FlyBase curator comment: The "l(1)19Ec" complementation group is stated to correspond to the "CG11233" annotation in FBrf0155430. However, data from FBrf0193836 suggests that the "l(1)19Ec" complementation group in fact corresponds to the "CG12092" annotation.

(Bhattacharya, 2003.2.12)

Other Comments

Mutant embryos from heterozygous females exhibit no defects in central or peripheral nervous systems; l(1)19Ec¹ and l(1)19Ec¹⁸ are lethal in germ-line clones, whereas l(1)19Ec⁸ and l(1)19Ec²⁵ germ-line clones exhibit a paternally rescuable maternal effect. Embryonic phenotype includes head and dorsal-closure defects and most embryos are twisted (Perrimon, Smouse, and Miklos, 1989).

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•