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Gene Dmel\Ank2

General Information
Symbol	Dmel\Ank2	Species	D. melanogaster
Name	Ankyrin 2	Annotation symbol	CG42734
Feature type	protein_coding_gene	FlyBase ID	FBgn0261788
Gene Model Status	Current	Stock availability	19 publicly available
Also Known As	CG7462, Dank2, CG34416, SP2523, CG32377, ESTS:149B10S
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	66A10-66A10	Sequence location	3L:7,648,489..7,711,495 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ankyrin 2 is referred to in FlyBase by the symbol Dmel\Ank2 (CG42734, FBgn0261788). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: structural constituent of cytoskeleton; cytoskeletal protein binding. There is experimental evidence that it is involved in the biological process: negative regulation of neuromuscular synaptic transmission; sensory perception of sound; neuron homeostasis; microtubule cytoskeleton organization; neuromuscular junction development; axon extension. 29 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/larval peripheral nervous system; NMJ bouton; neuromuscular junction; embryonic/larval neuromuscular junction. It has 17 annotated transcripts and 17 annotated polypeptides. Protein features are: Ankyrin repeat; Ankyrin repeat-containing domain; Death domain; Death-like domain; Protein translocase subunit SecF, bacterial; ZU5. Gene sequence location is 3L:7648489..7711495.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 66A10-66A10   Limits computationally determined from genome sequence between P{PZ}l(3)0721708223&P{EP}Rac2EP3118 and P{lacW}Nmtj1C7
Experimentally Determined Cytological Location
Cytogenetic map Notes References 66A-66A     (Hortsch, 1999.9.28) 66A8-66A9     (Serano, 2000.2.28) 66A-66A   (determined by in situ hybridisation)   (EDGP Project Members, 1994-)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Ank2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Gene model reviewed during 5.48 Gene model includes transcripts encoding non-overlapping portions of the full CDS. Gene merge based on RTPCR data (FBrf0204637) Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Ank2-RE FBtr0303120  NM_001104064   2780   697 Ank2-RF FBtr0303121  NM_001104065   13793   4114 Ank2-RG FBtr0303122  NM_001104066   8886   2532 Ank2-RJ FBtr0303123  NM_001104068   14018   4189 Ank2-RK FBtr0303124  NM_001104069   14309   4264 Ank2-RL FBtr0303117  NM_168227   13700   4083 Ank2-RM FBtr0303118  NM_139891   7820   2404 Ank2-RN FBtr0303119  NM_001104063   4752   1159 Ank2-RP FBtr0303111  NM_001202140   13764   4230 Ank2-RQ FBtr0303112  NM_001202138   14130   4352 Ank2-RR FBtr0303115  NM_001202136   15005   4496 Ank2-RS FBtr0303113  NM_001202135   14438   4329 Ank2-RT FBtr0303114  NM_001202139   14120   4223 Ank2-RU FBtr0303125  NM_001202141   42128   13559 Ank2-RV FBtr0334060  NM_001274606   14636   4373 Ank2-RW FBtr0334061  NM_001274607   5268   1309 Ank2-RX FBtr0334062  NM_001274608   2261   547
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Ank2-PE   FBpp0292239   75.7   697   7.85     NP_001097534   ABW08484 Ank2-PF   FBpp0292240   452.5   4114   4.64     NP_001097535   ABW08485 Ank2-PG   FBpp0292241   279.2   2532   5.69     NP_001097536   ABW08486 Ank2-PJ   FBpp0292242   460.7   4189   4.61     NP_001097538   ABW08487 Ank2-PK   FBpp0292243   468.9   4264   4.83     NP_001097539   ABW08488 Ank2-PL   FBpp0292236   448.9   4083   4.65     NP_729285   AAF50525 Ank2-PM   FBpp0292237   264.7   2404   6.12     NP_648148   AAN12046 Ank2-PN   FBpp0292238   125.8   1159   7.33     NP_001097533   ABW08483 Ank2-PP   FBpp0292230   464.8   4230   4.63     NP_001189069   ADV37506 Ank2-PQ   FBpp0292231   478.7   4352   4.59     NP_001189067   ADV37504 Ank2-PR   FBpp0292234   494.3   4496   4.74     NP_001189065   ADV37502 Ank2-PS   FBpp0292232   475.7   4329   4.58     NP_001189064   ADV37501 Ank2-PT   FBpp0292233   463.9   4223   4.62     NP_001189068   ADV37505 Ank2-PU   FBpp0292244   1488.3   13559   4.65     NP_001189070   ADV37507 Ank2-PV   FBpp0306185   480.4   4373   4.79     NP_001261535   AGB94230 Ank2-PW   FBpp0306186   142.2   1309   7.50     NP_001261536   AGB94231 Ank2-PX   FBpp0306187   59.3   547   7.74     NP_001261537   AGB94232
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Death-like domain (IPR011029) Ankyrin repeat (IPR002110) ZU5 (IPR000906) Death domain (IPR000488) Ankyrin repeat-containing domain (IPR020683)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567514   AA567906   AA568067   AA697588   AA698579   AA699044   AA801522   AE014296 AAF50525   AE014296 AAN12046   AE014296 ABW08483   AE014296 ABW08484   AE014296 ABW08485   AE014296 ABW08486   AE014296 ABW08487   AE014296 ABW08488   AE014296 ADV37501   AE014296 ADV37502   AE014296 ADV37504   AE014296 ADV37505   AE014296 ADV37506   AE014296 ADV37507   AF190635 AAF73309   AF239611 AAF63503   AE014296 AGB94230   AE014296 AGB94231   AE014296 AGB94232   AI062429   AI114072   AI134332   AI238518   AI238579   AI293336   AI403859   AI405205   AI405395   AI514204   AK281610   AW940057   AY051490 AAK92914   AY094999 AAM11327   BF492648   BF492649   BF493249   BF498582   BG632939   BG633451   BG635364   BG637649   BI374957   BI564777   BI565667   BI574548   BI627343   BT023884   BT058088   BT088796   BT133155   CO177436   CO185349   CO263526   CO267150   CO270209   CO279186   CO288810   CO288814   CO289249   CO289849   CO290540   CO291869   CO292213   CO297961   CO298053   CO298750   CO298979   CO301200   CO301399   CO301809   CO303495   CO306211   CO308173   CO308861   CO310787   CO313145   CO314905   CO315516   CO318877   CO319527   CO323705   CO324184   CO325739   CO328069   CO331695   CO333575   CO344881   CZ482977   EC059778   EC064725   EC065689   EC067969   EC079818   EC080080   EC081082   EC081752   EC082269   EC085378   EC086158   EC088569   EC089072   EC089345   EC091156   EC196303   EC198746   EC199356   EC200750   EC201095   EC203813   EC205883   EC205966   EC211355   EC211395   EC212333   EC214129   EC215746   EC215747   EC215752   EC215877   EC216528   EC219036   EC219037   EC219219   EC219679   EC221823   EC224014   EC234143   EC235156   EC235481   EC237108   EC237504   EC237917   EC238374   EC242155   EC242751   EC246113   EC250384   EC251914   EC252639   EC255391   EC256635   EC258287   EC258883   EL880079   EL884835   EL884959   EL885087   EL885088   EL885204   EL885205   EL886200   GR705748   Z83523
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KU95 Q9NCP8 Q7KU92 A8JNM4 Q3KN55 A8JNM6 A8JNM5 A8JNM7 Q8IQ87 Q0E8H3 Q9I7Q8 Q8SWY2 Q7KIS5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
expression microarray Stage Tissue/Position (including subcellular localization) Reference adult stage   eye   (Xu et al., 2004) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 12   ventral nerve cord primordium   (Bouley et al., 2000, Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) midline primordium   (Fisher et al., 2012) embryonic stage 13 -- 14   ventral nerve cord   (Bouley et al., 2000) embryonic brain   (Bouley et al., 2000) presumptive embryonic/larval peripheral nervous system   (Bouley et al., 2000) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral midline   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15 -- 17   organism   (Bouley et al., 2000) Comment:reference states 12-24 hr AEL P-stage   organism   (Bouley et al., 2000)
Additional Descriptive Data
	Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads. (Xu et al., 2004)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 12   ventral nerve cord primordium   (Bouley et al., 2000) embryonic stage 12 -- 15   presumptive embryonic/larval central nervous system   (Koch et al., 2008) presumptive embryonic/larval peripheral nervous system   (Koch et al., 2008) embryonic stage 13   VUM precursor   (Bouley et al., 2000) embryonic stage 13 -- 15   neuron of presumptive embryonic/larval central nervous system   (Hortsch et al., 2002) embryonic stage 13 -- 17   ventral nerve cord   (Bouley et al., 2000) embryonic brain   (Bouley et al., 2000) presumptive embryonic/larval peripheral nervous system   (Bouley et al., 2000) embryonic/larval neuron   • neuronal cell body (Bouley et al., 2000) embryonic/larval neuron   • axon (Bouley et al., 2000) embryonic stage 14 -- 17   sensory neuron   • axon (Bouley et al., 2000) embryonic stage 15   scolopidial neuron   (Hortsch et al., 2002) embryonic stage 16 -- 17   embryonic/larval central nervous system   (Koch et al., 2008) embryonic/larval peripheral nervous system   (Koch et al., 2008) neuromuscular junction   (Koch et al., 2008)   • neuromuscular junction (Koch et al., 2008) NMJ bouton   (Koch et al., 2008)   • terminal bouton (Koch et al., 2008) embryonic stage 17   segmental nerve   • axon (Bouley et al., 2000) intersegmental nerve   • axon (Bouley et al., 2000) second instar larval stage   embryonic/larval neuromuscular junction   • axon (Pielage et al., 2008) embryonic/larval neuromuscular junction   • terminal bouton (Pielage et al., 2008) wandering third instar larval stage   embryonic/larval neuromuscular junction   • axon (Pielage et al., 2008) embryonic/larval neuromuscular junction   • terminal bouton (Pielage et al., 2008) P-stage   photoreceptor cell   (Hortsch et al., 2002) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   • membrane (Bouley et al., 2000)
Additional Descriptive Data
	Ank2 isoform Ank2-PL is expressed in the axon and presynaptic bouton at the neuromuscular junction. In the axon, expression of Ank2-PL is closely associated with the membrane and with the microtubule-associated protein futsch, and organised into a lattice-like structure. In the presynaptic boutons, Ank2-PL isoform is less abundant and found adjacent to the presynaptic active zone marker brp and in close proximity to the synaptic cell adhesion marker Fas2. In the areas between synaptic boutons, Ank2-PL protein is more abundant and organised into a lattice-like pattern. (Pielage et al., 2008) The short isoform of Ank2 protein, i.e., Ank-PI, is localized to neuronal cell bodies, whereas antibodies to long isoforms stain primarily axonal pathways. (Hortsch et al., 2002) Different Ank2 isoforms show different subcellular localizations. They are expressed in the embryonic central and peripheral nervous systems. Ank2 is found at neuromuscular junctions both between and within synaptic boutons. It is expressed in periactive zones in neruomuscular junctions. (Koch et al., 2008)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
neuromuscular junction inferred from direct assay (Koch et al., 2008) plasma membrane inferred from direct assay (Khanna et al., 2010) presynaptic membrane inferred from direct assay (Koch et al., 2008, Pielage et al., 2008) presynaptic periactive zone inferred from direct assay (Koch et al., 2008) terminal bouton inferred from direct assay (Pielage et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
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modENCODE Development RNA-Seq
[an error occurred while processing this directive] modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
[an error occurred while processing this directive]
modENCODE Treatments RNA-Seq
[an error occurred while processing this directive]
Expression Clusters
External Data & Images
Linkouts	BDGP expression data • GH14650
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Df(3L)RM5-2 Ank2f02001 lethal (with Ank2f00518) Ank2f02001 lethal (with Ank2f02001) Ank2f00518 lethal | larval stage, with Df(3L)RM5-2 Ank2f00518 lethal | larval stage | recessive Ank2f00518 lethal | recessive Ank2f02001 viable, with Scer\GAL4pnr-MD237 Ank2GD10869 Ank2GD12247 Ank2GD16285 Other Phenotypes Allele auditory perception defective | adult stage Ank2f02001 neuroanatomy defective Ank2f00518 Ank2f02001 neuroanatomy defective, with Df(3L)RM5-2 Ank2f00518 Ank2f02001 neuroanatomy defective, with Scer\GAL4IG1-1 Ank2dsRNA.Scer\UAS neuroanatomy defective, with Scer\GAL4unspecified Ank2dsRNA.L.Scer\UAS Ank2L8.Scer\UAS.T:Avic\GFP-YFP.Venus neuroanatomy defective (with Ank2f00518) Ank2f02001 neuroanatomy defective (with Ank2f02001) Ank2f00518 neuroanatomy defective | larval stage (with Ank2K327) Ank2RM5-1 neuroanatomy defective | larval stage (with Ank2RM5-1) Ank2K327 neuroanatomy defective | third instar larval stage Ank2f02001 neuroanatomy defective | third instar larval stage (with Ank2E380) Ank2K327 neuroanatomy defective | third instar larval stage (with Ank2f02001) Ank2RM5-1 neuroanatomy defective | third instar larval stage (with Ank2K327) Ank2E380 neuroanatomy defective | third instar larval stage (with Ank2RM5-1) Ank2f02001 neurophysiology defective Ank2f02001 neurophysiology defective | second instar larval stage, with Df(3L)RM5-2 Ank2f00518 neurophysiology defective | third instar larval stage, with Df(3L)RM5-2 Ank2f02001 Phenotype manifest in Allele axon | third instar larval stage Ank2f02001 dorsal multidendritic neuron ddaE & axon, with Scer\GAL4IG1-1 Ank2dsRNA.Scer\UAS embryonic/larval neuromuscular junction (with Ank2E380) Ank2K327 embryonic/larval neuromuscular junction (with Ank2f02001) Ank2RM5-1 embryonic/larval neuromuscular junction (with Ank2K327) Ank2E380 Ank2RM5-1 embryonic/larval neuromuscular junction (with Ank2RM5-1) Ank2f02001 Ank2K327 embryonic/larval neuromuscular junction | third instar larval stage Ank2f02001 embryonic/larval peripheral nervous system (with Ank2E380) Ank2K327 embryonic/larval peripheral nervous system (with Ank2K327) Ank2E380 microtubule | third instar larval stage Ank2f02001 neuromuscular junction Ank2f00518 Ank2f02001 neuromuscular junction, with Df(3L)RM5-2 Ank2f00518 Ank2f02001 neuromuscular junction, with Scer\GAL4unspecified Ank2dsRNA.L.Scer\UAS Ank2L8.Scer\UAS.T:Avic\GFP-YFP.Venus neuromuscular junction (with Ank2f00518) Ank2f02001 neuromuscular junction (with Ank2f02001) Ank2f00518 NMJ bouton Ank2f02001 NMJ bouton, with Df(3L)RM5-2 Ank2f00518 Ank2f02001 NMJ bouton, with Scer\GAL4unspecified Ank2L8.Scer\UAS.T:Avic\GFP-YFP.Venus NMJ bouton (with Ank2E380) Ank2K327 NMJ bouton (with Ank2f02001) Ank2RM5-1 NMJ bouton (with Ank2K327) Ank2E380 NMJ bouton (with Ank2RM5-1) Ank2f02001
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of Ank2 Class Mutagen Stocks Known lesion Ank2f02001 2 -- Ank2f00518 piggyBac transposase 1 -- Ank2MB00005 1 -- Ank2MB02773 1 -- Ank2MB04517 1 -- Ank2MI01704 1 -- Ank2MI02111 1 -- Ank2MI02275 1 -- Ank2E380 ethyl methanesulfonate 0 Yes Ank2I864 ethyl methanesulfonate 0 Yes Ank2K327 ethyl methanesulfonate 0 Yes Ank2RM5-1 0 Yes
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ank2 Class Mutagen Stocks Known lesion Ank2GD10869 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Ank2GD16285 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Ank2GD12247 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ank2HMS00295 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ank2JF03374 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ank2KK104937 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ank2KK106729 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ank2KK113612 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ank2dsRNA.L.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ank2dsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes Ank2GD9649 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ank2L4.Scer\UAS.T:Avic\GFP-YFP.Venus in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes Ank2L8.Scer\UAS.T:Avic\GFP-YFP.Venus in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes Ank2NIG.32377R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ank2NIG.7462R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ank2S.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Ank2S.Scer\UAS.T:Avic\GFP-YFP.Venus in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(3L)HnD-1 (Koch et al., 2008) Df(3L)pbl-NR (Koch et al., 2008) Df(3L)XDI98 (Koch et al., 2008) Df(3L)ZP1 (Koch et al., 2008)
Disrupted in	Df(3L)pbl-X1 (Koch et al., 2008) Df(3L)RM5-1 (Koch et al., 2008) Df(3L)RM5-2 (Koch et al., 2008)
Partially disrupted in	Df(3L)RM5-1 (Koch et al., 2008)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9649} NA P{GD10869} NA P{GD12247} NA P{KK104937} NA P{KK106729} NA P{KK113612} NA P{NIG.7462R} NA P{NIG.32377R} NA P{TRiP.HMS00295} NA P{TRiP.JF03374} NA P{UAS-Ank2.RNAi} NA P{UAS-Ank2.S.EGFP} NA P{UAS-Ank2-L.RNAi} NA P{UAS-YFP-Ank2-L4} NA P{UAS-YFP-Ank2-L8} NA P{UAS-YFP-Ank2-S} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}Ank2MI01704 NA Mi{MIC}Ank2MI02111 NA Mi{MIC}Ank2MI02275 NA PBac{WH}Ank2f00518 NA PBac{WH}Ank2f02001 NA insertion of enhancer trap binary system Mi{ET1}Ank2MB04517 No Mi{ET1}CG32373MB00005 No Mi{ET1}CG32373MB02773 No
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 11 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
axon extension inferred from mutant phenotype (Yamamoto et al., 2006) microtubule cytoskeleton organization inferred from mutant phenotype (Koch et al., 2008, Pielage et al., 2008) negative regulation of neuromuscular synaptic transmission inferred from mutant phenotype (Keller et al., 2011) neuromuscular junction development inferred from mutant phenotype (Koch et al., 2008, Pielage et al., 2008) neuron homeostasis inferred from genetic interaction with Ank2 (Keller et al., 2011) sensory perception of sound inferred from mutant phenotype (Senthilan et al., 2012)
Cellular Component
CV term Evidence References
neuromuscular junction inferred from direct assay (Koch et al., 2008) plasma membrane inferred from direct assay (Khanna et al., 2010) presynaptic membrane inferred from direct assay (Koch et al., 2008, Pielage et al., 2008) presynaptic periactive zone inferred from direct assay (Koch et al., 2008) terminal bouton inferred from direct assay (Pielage et al., 2008)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
cytoskeletal protein binding inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) structural constituent of cytoskeleton inferred from sequence or structural similarity with EMBL:L40632 (FlyBase, 1992-) non-traceable author statement (Hortsch, 1999.9.28)
Biological Process
CV term Evidence References
cytoskeletal anchoring at plasma membrane inferred from sequence or structural similarity (EDGP Project Members, 1994-) non-traceable author statement (Hortsch, 1999.9.28) signal transduction inferred from electronic annotation with InterPro:IPR000488 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
plasma membrane inferred from sequence or structural similarity (EDGP Project Members, 1994-)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ank2 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261788 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38863 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • A8JNM5
Orthologs
OrthoDB Orthologs (62) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6RNBTP Dipteran inclusive ortholog search EOG6DV5V6, EOG683DCH Insect inclusive ortholog search EOG63XSZM, EOG6B5N0Z Arthropod inclusive ortholog search EOG6DR7TG, EOG6C5B0C Metazoa inclusive ortholog search EOG6R5BVJ
Orthologs in Drosophila Species (EOG6RNBTP)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Ank2     Drosophila sechellia   Dsec\GM13739     Drosophila persimilis   Dper\GL24099
Orthologs in non-Drosophila Dipterans (EOG6DV5V6, EOG683DCH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011565 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014668 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013466 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014742 Y Anopheles gambiae Malaria mosquito  Agam\AGAP009937 Y Anopheles gambiae Malaria mosquito  Agam\AGAP002272 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017156 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010503 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002592 Y
Orthologs in non-Dipteran Insects (EOG63XSZM, EOG6B5N0Z)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB44218 Y Apis mellifera Western honey bee  Amel\GB46564 Y Apis mellifera Western honey bee  Amel\GB47425 Y Apis mellifera Western honey bee  Amel\GB45715 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010051 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011990 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15861 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH21619 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17403 Y Atta cephalotes Leafcutter ant  Acep\ACEP11003 Y Atta cephalotes Leafcutter ant  Acep\ACEP16179 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO14623 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO18535 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13195 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL10487 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL26919 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL11243 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL11614 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19388 Y Linepithema humile Argentine ant  Lhum\LH17900 Y Linepithema humile Argentine ant  Lhum\LH17830 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB24205 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB25065 Y Solenopsis invicta Red fire ant  Sinv\SINV15011 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI38268 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI000387 Y Pediculus humanus Human body louse  Phum\PHUM596790 Y Pediculus humanus Human body louse  Phum\PHUM570540 Y Tribolium castaneum Red flour beetle  Tcas\TC003843 Y Tribolium castaneum Red flour beetle  Tcas\TC003960 Y
Orthologs in non-Insect Arthropods (EOG6DR7TG, EOG6C5B0C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\PHYP Y Daphnia pulex Water flea  Dpux\CYB561D1 Y Daphnia pulex Water flea  Dpux\COII Y Ixodes scapularis Deer tick  Isca\ISCW018406 Y
Orthologs in non-Arthropod Metazoa (EOG6R5BVJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\clx-1 Y Caenorhabditis elegans Nematode  Cele\unc-44 Y Caenorhabditis elegans Nematode  Cele\K02E11.10 Y Caenorhabditis elegans Nematode  Cele\K06A9.1 Y Caenorhabditis elegans Nematode  Cele\col-50 Y Caenorhabditis elegans Nematode  Cele\F16B4.4 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000908gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021014gn Y Danio rerio Zebrafish  Drer\si:ch211-173b16.3 Y Danio rerio Zebrafish  Drer\OTTDARG00000031570 Y Danio rerio Zebrafish  Drer\CR388002.1 Y Xenopus tropicalis Western clawed frog  Xtro\ank1 Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000003594 Y Mus musculus House mouse  Mmus\ENSMUSG00000031543 Y Rattus norvegicus Norway rat  Rnor\Ank1 Y Homo sapiens Human  Hsap\ANK1 Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\ANK1
AAA Orthologs (3) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM22068 Drosophila persimilis Dper\GL24077 Drosophila mojavensis Dmoj\GI13079
Stocks & Reagents
Stocks Listed in FlyBase ( 19 )
Bloomington	29438 y1 v1; P{TRiP.JF03374}attP2 23488 w1118; Mi{ET1}CG32373MB02773 Ank2MB02773
Harvard	- PBac{WH}Ank2f00518 - PBac{WH}Ank2f02001
VDRC	v104833 P{KK113612}VIE-260B v107238 P{KK104937}VIE-260B v107369 P{KK106729}VIE-260B v40638 w1118 P{GD12247}v40638 v26121 w1118; P{GD10869}v26121 v26122 w1118; P{GD10869}v26122 v46224 w1118; P{GD16285}v46224 v46225 w1118; P{GD16285}v46225
	More stocks available...
Genomic Clones ( 1 )
	BACR03I22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 119 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH01626 RE03629 RE55168
Other clones	FI18613 MIP05285
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01564 AT13020 AT31929 GH10748 GH14574 GH23389 GH25141 GH25381 HL01245 HL01973 HL02830 HL04961 HL06523 HL07354 RE62654 RH24481 RH62282 RH63474 RH68629
Other clones	18965 34146 47056 53664 54815 55337 58146 64547 64794 64976 70172 70604 70605 99939 106107 108556 119135 121341 146461 153425 156480 158833 160392 174005 174051 175612 214217.72 214313.69 214313.70 214479.74 214479.75 214499.75 273999 282150 AT01115 CK01.4A.H4 CK01.4C.H4 CK01.12A.C3 CK01.18A.B8 CK01.64C.H8 CK01.88A.F1 CK01.88A.G1 CK01.89C.H8 CK01.91B.G9 CK01.94D.F6 CK01.95B.E12 CK01.106B.A2 CK01.108C.E7 CK01.117C.A10 CK01.128C.G6 EK008362 EK053446 EK069421 EK069425 EK070546 EK072227 EK074250 EK088733 EK097920 EK133728 EK148853 EK171017 EK171225 EK173764 EK174229 EK180418 EK180862 EK182223 EK191215 EK192931 EK197349 EK198549 EK222522 EK231713 EK238331 EK250527 EK256913 EK259534 EK261726 EK274707 EK278717 EK301126 EK307328 EP08523 EP20127 GH16529 GH27281 HL01375 IP09921 IP16062 IP16162 IP16262 IP16362 IP17479 MB6-CG34416-RG
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261788 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38863
Antibody Information
	polyclonal (Koch et al., 2008, Hortsch et al., 2002, Bouley et al., 2000)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: SP2523 CG7493 (FlyBase, 1996-)
Source for database merge of	Source for merge of: Ank2 CG32377 (Koch et al., 2008) Source for merge of: Ank2 SP2523 CG32375 CG34121 (FlyBase Genome Annotators, 2007)
Additional comments	Annotations CG34416 and CG32377 merged as CG42734 (which corresponds to Ank2) in release 5.27 of the genome annotation. (FlyBase Genome Annotators, 2010) Annotations CG7462, CG32375, CG34158 and CG34121 merged as CG34416 in release 5.2 of the genome annotation. (FlyBase Genome Annotators, 2007) FlyBase curator comment: The symbol "CG18768" has been used erroneously in FlyBase to name 2 adjacent annotations in different releases of the genome annotation. The two annotations have been renamed in release 5.1 to avoid confusion; they are CG34158 (which corresponds to SP2523) and CG34121. CG34121 was labelled "CG18768" in release 2 of the genome annotation and was not annotated in releases 3.0 through 4.3, but has been reinstated (as CG34121) in release 5.1 of the genome annotation. CG34158 (which corresponds to SP2523) was labelled "CG7493" in release 2 of the genome annotation, was labelled "CG18768" in releases 3.0 through 4.3, and has been renamed CG34158 in release 5.1 of the genome annotation. (FlyBase Genome Annotators, 2006) Source for identity of SP2523 CG7493 was sequence comparison (date:000414). (FlyBase, 1996-)
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) New annotation (CG32375) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) New annotation (CG32377) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Identification: as a protein that interacts with both Nrg isoforms in a yeast two-hybrid assay. (Bouley et al., 2000) The existence of this gene is tentative, and is based on a match of an STS sequence from a European Drosophila Genome Mapping Project cosmid.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_139891 NM_168227 NM_001104063 NM_001104064 NM_001104065 NM_001104066 NM_001104068 NM_001104069 NM_001202135 NM_001202136 NM_001202138 NM_001202139 NM_001202140 NM_001202141 NM_001274606 NM_001274607 NM_001274608 RefSeq (Proteins) • NP_648148 NP_729285 NP_001097533 NP_001097534 NP_001097535 NP_001097536 NP_001097538 NP_001097539 NP_001189064 NP_001189065 NP_001189067 NP_001189068 NP_001189069 NP_001189070 NP_001261535 NP_001261536 NP_001261537 DDBJ / EMBL / GenBank • AAF50525 AAF63503 AAF73309 AAG22324 AAK92914 AAM11327 AAN12045 AAN12046 ABI31241 ABW08483 ABW08484 ABW08485 ABW08486 ABW08487 ABW08488 ADV37501 ADV37502 ADV37503 ADV37504 ADV37505 ADV37506 ADV37507 AF190635 AF239611 AGB94230 AGB94231 AGB94232 AI062429 AI238579 AW940057 AY051490 AY094999 CZ482977 Z83523 Entrez Gene - A searchable database of RefSeq genes. • 38863 318003 UniProtKB/TrEMBL • A8JNM4 A8JNM5 A8JNM6 A8JNM7 Q0E8H3 Q3KN55 Q7KIS5 Q7KU92 Q7KU95 Q8IQ87 Q8SWY2 Q9I7Q8 Q9NCP8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Death-like domain (IPR011029) Ankyrin repeat (IPR002110) ZU5 (IPR000906) Death domain (IPR000488) Ankyrin repeat-containing domain (IPR020683) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH14650
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261788 DRSC - Results from RNAi screens. • FBgn0261788 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261788 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 38863 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 38863 modMine - Data generated by the modENCODE project. • FBgn0261788 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • A8JNM5
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	Ank2 (Banovic et al., 2010, Yamamoto, 2006, Mazock et al., 2010, Wasbrough et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Senthilan et al., 2012, Cingolani et al., 2012) ank2 (Kurshan et al., 2009, Yamamoto et al., 2006, Pielage et al., 2008, Koch et al., 2008, Keller et al., 2011, Nechipurenko and Broihier, 2012) ank2-L (Massaro et al., 2009) CG7462 (Hortsch, 2002.5.1, Hortsch, 2002.5.1, Hortsch, 2002.5.1, Hortsch, 2002.5.1, Goldstein and Gunawardena, 2000, Vernooy et al., 2000, ) CG7493   CG18025   CG18227   CG18768   CG32373 (Pielage et al., 2008) CG32375   CG32377 (Koch et al., 2007, Koch et al., 2008, Japanese National Institute of Genetics, 2012.5.21) CG34121   CG34158 (FlyBase Genome Annotators, 2006) CG34416 (Perkins et al., 2009, FlyBase Genome Annotators, 2007, Keleman et al., 2009.8.5, Khanna et al., 2010) CG42734 (FlyBase Genome Annotators, 2010, Ni et al., 2010.12.1) Dank2 (Hortsch et al., 2002, Bouley et al., 2000, Hortsch et al., 2002) dank2 (Koch et al., 2007) DAnk2 (Mazock et al., 2010) ESTS:149B10S (Bolshakov et al., 2002, EDGP Project Members, 1994-, ) SP2523 (Koch et al., 2008, Koch et al., 2007, Pielage et al., 2008) sp2523 (Michaut et al., 2003)
Name Synonym	Ank2   Ankyrin 2 (Banovic et al., 2010, Khanna et al., 2010) ankyrin2 (Keller et al., 2011) ankyrin2-XL (Kurshan et al., 2009) CG32375   CG34121   Dank2 (Hortsch et al., 2002) SP2523
Secondary FlyBase IDs
	FBgn0017645 FBgn0035808 FBgn0035809 FBgn0035810 FBgn0042181 FBgn0052375 FBgn0052377 FBgn0083957 FBgn0083993 FBgn0085445 FBgn0261687
References ( 61 )
Generate a list of	All references relating to this gene ( 61 )
List References by type	Research paper  ( 20 ) Supplementary material  ( 9 ) Personal communication to FlyBase  ( 18 ) Abstract  ( 2 ) FlyBase analysis  ( 6 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 2 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 5 )
	Cingolani et al., 2012, Front. Genet. 3: 35 Using Drosophila melanogaster as a Model for Genotoxic Chemical Mutational Studies with a New Program, SnpSift. [FBrf0217795] Nechipurenko and Broihier, 2012, J. Cell Biol. 196(3): 345--362 FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. [FBrf0217391] Senthilan et al., 2012, Cell 150(5): 1042--1054 Drosophila auditory organ genes and genetic hearing defects. [FBrf0219321] Keller et al., 2011, Neuron 72(5): 760--775 Glial-derived prodegenerative signaling in the Drosophila neuromuscular system. [FBrf0216904] Pielage et al., 2011, Neuron 69(6): 1114--1131 Hts/Adducin controls synaptic elaboration and elimination. [FBrf0213329] Show all research papers ...