Gene Dmel\Dhc64C

64C-64C

(determined by in situ hybridisation)

(Li et al., 1992)

Experimentally Determined Recombination Data

Location

3-10 +/- 4

(Harte and Kankel, 1982, Rasmusson, 1994.7.28)

Left of (cM)

(Harte and Kankel, 1982, Belecz et al., 2001)

Right of (cM)

(Harte and Kankel, 1982, Belecz et al., 2001)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Dhc64C for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

DGC clone GH15453 appears problematic: incomplete CDS

Evidence indicates that 3' UTR overlaps 5' UTR of downstream gene; extends to coordinate AE003482:167309 (based upon 3' extent of accession L23195)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Dhc64C-RA

FBtr0073359

NM_079205

14381

4639

Dhc64C-RC

FBtr0273370

NM_168103

14378

4638

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Dhc64C-PA

FBpp0073215

530.2

4639

6.25

NP_523929

AAF47942

Dhc64C-PC

FBpp0271878

529.9

4638

6.24

NP_729034

AAN11615

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Dynein heavy chain, P-loop containing D4 domain (IPR024317)

Dynein heavy chain, coiled coil stalk (IPR024743)

ATPase, AAA+ type, core (IPR003593)

Dynein heavy chain (IPR004273)

ATPase, dynein-related, AAA domain (IPR011704)

Dynein heavy chain, domain-1 (IPR013594)

Dynein heavy chain, domain-2 (IPR013602)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P37276

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

adult stage | female

ovary

adult stage | male

testis

Additional Descriptive Data

Dhc64C is the main dynein transcript expressed in ovaries and embryos. It is also detected in male testis.

Marker for

Subcellular Localization

CV Term

Notes

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female & oogenesis stage S9

oocyte | posterior

• cell cortex

(Loiseau et al., 2010)

stage S10A oocyte | posterior

(Brendza et al., 2002)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

High-Throughput Expression Data

Untitled Document detailed view

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

Expression Clusters

External Data & Images

increased cell death | larval stage

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

cell lethal | somatic clone

lethal, with Scer\GAL4^pnr-MD237

Dhc64C^GD12258

lethal | embryonic stage

lethal | embryonic stage | maternal effect

Dhc64C^Lab-1

lethal | embryonic stage | maternal effect | recessive

lethal | first instar larval stage (with Df(3L)10^H)

Dhc64C^γ4163a

lethal | larval stage | recessive

Dhc64C^4-19

lethal | pharate adult stage (with Df(3L)10^H)

Dhc64C^6-10

lethal | pupal stage

Dhc64C^6-10

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Dhc64C^GD12258

viable (with Dhc64C^6-6)

Dhc64C^6-12

viable (with Dhc64C^6-8)

Dhc64C¹⁰²

viable (with Dhc64C^6-10)

Dhc64C¹⁰²

viable (with Dhc64C^6-12)

Dhc64C^6-6

viable (with Dhc64C¹⁰²)

Sterility

Allele

female sterile (with Df(3L)10^H)

Dhc64C^X-18

female sterile (with Dhc64C^6-6)

Dhc64C^6-12

female sterile (with Dhc64C^6-12)

Dhc64C^6-6

female sterile | dominant

Dhc64C^Lab-1

Other Phenotypes

Allele

cell polarity defective

Dhc64C⁹⁰²

cell shape defective | adult stage | somatic clone

Dhc64C^6-10

mitotic cell cycle defective

Dhc64C^dsRNA.cMa

mitotic cell cycle defective (with Df(3L)10^H)

Dhc64C^6-10

mitotic cell cycle defective | larval stage

Dhc64C^6-10

neuroanatomy defective (with Dhc64C^4-19)

Dhc64C^6-10

neuroanatomy defective (with Dhc64C^6-10)

Dhc64C^4-19

neuroanatomy defective | somatic clone

Dhc64C^4-19

neurophysiology defective (with Dhc64C^4-19)

Dhc64C^6-10

neurophysiology defective (with Dhc64C^6-10)

Dhc64C^4-19

paralytic | larval stage | recessive

wild-type

Dhc64C^+tDN17

Phenotype manifest in

Allele

adult mushroom body | somatic clone

Dhc64C^4-19

blastoderm embryo | maternal effect

border follicle cell | somatic clone

Dhc64C^4-19

centrosome

centrosome | germline clone

Dhc64C^greco

centrosome | maternal effect

chaeta (with Df(3L)10^H)

Dhc64C^X-18

cycle 14 embryo (with Dhc64C^6-6)

Dhc64C^6-8

cycle 14 embryo (with Dhc64C^6-8)

Dhc64C^6-6

cystocyte

cytoplasmic microtubule

Dhc64C^dsRNA.cGa

dendrite | somatic clone

Dhc64C^4-19

egg

egg chamber

embryonic/first instar larval cuticle (with Dhc64C^6-6)

Dhc64C^6-8

embryonic/first instar larval cuticle (with Dhc64C^6-8)

Dhc64C^6-6

embryonic/larval brain

Dhc64C^6-10

embryonic/larval neuroblast

Dhc64C^6-10

embryonic/larval neuromuscular junction (with Dhc64C^4-19)

Dhc64C^6-10

embryonic/larval neuromuscular junction (with Dhc64C^6-10)

Dhc64C^4-19

female pronucleus | maternal effect

Dhc64C^Lab-1

follicle cell

Dhc64C⁹⁰²

follicle cell | somatic clone

fusome

fusome | germline clone

Dhc64C^greco

germline cyst | germline clone (with Dhc64C^6-6)

Dhc64C^6-12

germline cyst | germline clone (with Dhc64C^6-12)

Dhc64C^6-6

Golgi apparatus | embryonic cycle 14 (with Dhc64C^6-6)

Dhc64C^6-8

Golgi apparatus | embryonic cycle 14 (with Dhc64C^6-8)

Dhc64C^6-6

kinetochore microtubule

Dhc64C^dsRNA.cMa

larval mushroom body | somatic clone

Dhc64C^4-19

lipid droplet

lipid droplet | maternal effect (with Dhc64C^6-10)

Dhc64C^8-1

lipid droplet | maternal effect (with Dhc64C^8-1)

Dhc64C^6-10

metaphase & condensed nuclear chromosome

Dhc64C^dsRNA.cGa

mitochondrial cloud | germline clone (with Dhc64C^6-6)

Dhc64C^6-12

mitochondrial cloud | germline clone (with Dhc64C^6-12)

Dhc64C^6-6

mitochondrion

Dhc64C^4-19

mitochondrion (with Dhc64C^4-19)

Dhc64C^6-10

mitochondrion (with Dhc64C^6-10)

Dhc64C^4-19

mitotic anaphase (with Df(3L)10^H)

Dhc64C^6-10

mitotic cell cycle

Dhc64C^Lab-1

mitotic cell cycle (with Df(3L)10^H)

Dhc64C^6-10

mitotic cell cycle | maternal effect

mitotic metaphase (with Df(3L)10^H)

Dhc64C^6-10

NMJ bouton (with Dhc64C^4-19)

Dhc64C^6-10

NMJ bouton (with Dhc64C^6-10)

Dhc64C^4-19

nucleus | embryonic stage 4

Dhc64C^unspecified

ommatidium (with Dhc64C^3-2)

Dhc64C^6-10

ommatidium (with Dhc64C^6-10)

Dhc64C^3-2

oocyte

oocyte (with Dhc64C^6-6)

Dhc64C^6-12

oocyte (with Dhc64C^6-12)

Dhc64C^6-6

oocyte | germline clone

Dhc64C^6-10

ovary (with Dhc64C^6-6)

Dhc64C^6-12

ovary (with Dhc64C^6-12)

Dhc64C^6-6

pole cell (with Dhc64C^6-6)

Dhc64C^6-10

pole cell (with Dhc64C^6-10)

Dhc64C^6-6

posterior fascicle & axon

Dhc64C^6-10

S2 cell-line

Dhc64C^dsRNA.cGa

spermatid cyst

Dhc64C^4-19

spermatocyte fusome

Dhc64C^4-19

spindle

spindle | maternal effect

spindle pole

Dhc64C^dsRNA.cMa

synapse (with Dhc64C^4-19)

Dhc64C^6-10

synapse (with Dhc64C^6-10)

Dhc64C^4-19

synaptonemal complex | germline clone

Dhc64C^greco

Classical Alleles ( 34 )

For All Classical Alleles Show

Allele of Dhc64C	Class	Mutagen	Stocks	Known lesion
Dhc64C^4-19	amorphic allele - genetic evidence, loss of function allele	ethyl methanesulfonate	3	Yes
Dhc64C^KG08838		P-element activity	2	--
Dhc64C^6-10	loss of function allele, hypomorphic allele - genetic evidence	ethyl methanesulfonate	1	Yes
Dhc64C^6-6	loss of function allele, hypomorphic allele - genetic evidence	ethyl methanesulfonate	1	Yes
Dhc64C^greco	amorphic allele - genetic evidence		0	--
Dhc64C^1-1	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C¹⁰²	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
Dhc64C^2-2			0	--
Dhc64C^3-2	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^4-16	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^4-18	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^4-22	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^4-3	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^4-6	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^5-12	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^5-6	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^5-7	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^5-8	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^6-12	loss of function allele, hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	Yes
Dhc64C^6-4	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C^6-6-16	hypomorphic allele - genetic evidence		0	--
Dhc64C^6-8	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C⁷⁷		ethyl methanesulfonate	0	--
Dhc64C^8-1	loss of function allele	ethyl methanesulfonate	0	Yes
Dhc64C⁹⁰²		ethyl methanesulfonate	0	Yes
Dhc64C^D12-5	hypomorphic allele - molecular evidence	ethyl methanesulfonate	0	--
Dhc64C^ek1	antimorphic allele - genetic evidence	ethyl methanesulfonate	0	--
Dhc64C^Lab-1		ethyl methanesulfonate	0	--
Dhc64C^N22-1		ethyl methanesulfonate	0	--
Dhc64C^Q43-4		ethyl methanesulfonate	0	--
Dhc64C^S027714			0	--
Dhc64C^unspecified			0	--
Dhc64C^X-18			0	--
Dhc64C^γ4163a		gamma ray	0	--

Alleles Carried on Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Dhc64C	Mutagen	Stocks	Known lesion
Dhc64C^GD12258	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Dhc64C^JF03177	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Dhc64C^+tDN17	in vitro construct - genomic fragment	0	Yes
Dhc64C^110-C.hs	in vitro construct - regulatory fusion	0	Yes
Dhc64C^138-N.hs	in vitro construct - regulatory fusion	0	Yes
Dhc64C^160-M.hs	in vitro construct - regulatory fusion	0	Yes
Dhc64C^180-N.hs	in vitro construct - regulatory fusion	0	Yes
Dhc64C^390-N.hs	in vitro construct - regulatory fusion	0	Yes
Dhc64C^400-C.hs	in vitro construct - regulatory fusion	0	Yes
Dhc64C^65-M.hs	in vitro construct - regulatory fusion	0	Yes
Dhc64C^dsRNA.cGa	in vitro construct - RNAi	0	Yes
Dhc64C^dsRNA.cMa	in vitro construct - RNAi	0	Yes
Dhc64C^{hs.T:Ivir\HA1}	in vitro construct - regulatory fusion	0	Yes
Dhc64C^{P1.T:Ivir\HA1}	in vitro construct - coding region fusion in vitro construct - site directed mutagenesis	0	Yes
Dhc64C^P1	in vitro construct - site directed mutagenesis	0	Yes
Dhc64C^{P1P3.T:Ivir\HA1}	in vitro construct - coding region fusion in vitro construct - site directed mutagenesis	0	Yes
Dhc64C^P1P3	in vitro construct - site directed mutagenesis	0	Yes
Dhc64C^{P3.T:Ivir\HA1}	in vitro construct - coding region fusion in vitro construct - site directed mutagenesis	0	Yes
Dhc64C^P3	in vitro construct - site directed mutagenesis	0	Yes
Dhc64C^T:Ivir\HA1	in vitro construct - coding region fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3L)10^H

(Gepner et al., 1996)

Df(3L)BSC436

(Christensen et al., 2008.4.15)

Df(3L)Exel6102

(Horne-Badovinac and Bilder, 2008, Ryder, 2004.4.26)

Df(3L)GN24

(Ghosh-Roy et al., 2004)

Duplicated in

Dp(3;3)BK7

(Belecz et al., 2001)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

heat-shock construct

UAS construct

reporter construct

characterization construct

P{Dhc⁺}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}Dhc64C^KG08838

Gene Ontology: Function, Process & Cellular Component ( 49 unique terms )

Terms Based on Experimental Evidence ( 38 terms )

Molecular Function

CV term

References

ATPase activity, coupled

inferred from direct assay

microtubule motor activity

inferred from direct assay

Biological Process

CV term

References

axon cargo transport

inferred from mutant phenotype

cellularization

inferred from mutant phenotype

(Maiato et al., 2004, Morales-Mulia and Scholey, 2005)

centrosome cycle

inferred from mutant phenotype

(Belecz et al., 2001)

centrosome localization

inferred from mutant phenotype

chitin-based cuticle development

inferred from genetic interaction with sdt

cystoblast division

inferred from mutant phenotype

(Snapp et al., 2004)

dendrite morphogenesis

inferred from mutant phenotype

establishment of epithelial cell apical/basal polarity

inferred from direct assay

(Li et al., 2008)

establishment of Golgi localization

inferred from mutant phenotype

establishment or maintenance of epithelial cell apical/basal polarity

inferred from mutant phenotype

germarium-derived oocyte fate determination

inferred from mutant phenotype

intracellular protein transport

inferred from mutant phenotype

(Siller et al., 2005)

maintenance of RNA location

inferred from direct assay

(Delanoue and Davis, 2005)

microtubule-based movement

inferred from direct assay

mitochondrion distribution

inferred from mutant phenotype

(Cox and Spradling, 2006)

mitochondrion transport along microtubule

inferred from mutant phenotype

(Pilling et al., 2006)

mitosis

inferred from mutant phenotype

(Maiato et al., 2004)

mitotic spindle organization

inferred from mutant phenotype

(Maiato et al., 2004, Goshima et al., 2007)

mitotic spindle stabilization

inferred from mutant phenotype

(Maiato et al., 2004)

mushroom body development

inferred from mutant phenotype

neuroblast proliferation

inferred from mutant phenotype

oogenesis

inferred from mutant phenotype

ovarian fusome organization

inferred from mutant phenotype

(Snapp et al., 2004)

pole cell formation

inferred from mutant phenotype

(Lerit and Gavis, 2011)

positive regulation of mitotic centrosome separation

inferred from mutant phenotype

(Maiato et al., 2004)

regulation of mitotic metaphase/anaphase transition

inferred from mutant phenotype

(Griffis et al., 2007)

RNA transport

inferred from mutant phenotype

(Clark et al., 2007)

spindle organization

inferred from mutant phenotype

(Morales-Mulia and Scholey, 2005)

Cellular Component

CV term

References

cell cortex

inferred from direct assay

(Swan et al., 1999)

cytoplasm

inferred from direct assay

dynein complex

inferred from physical interaction with Dlic

(Satoh et al., 2008)

Golgi apparatus

inferred from direct assay

microtubule associated complex

kinetochore

inferred from direct assay

(Griffis et al., 2007)

inferred from direct assay

(Hays et al., 1994, Hughes et al., 2008)

colocalizes_with
mitochondrion

inferred from direct assay

(Pilling et al., 2006)

ribonucleoprotein complex

inferred from direct assay

(Boylan et al., 2008)

Terms Based on Predictions or Assertions ( 17 terms )

Molecular Function

CV term

(Goldstein and Gunawardena, 2000)

References

ATPase activity, coupled

inferred from sequence or structural similarity

non-traceable author statement

(Swiss-Prot Project Members, 1994.10.1)

ATP binding

inferred from electronic annotation with InterPro:IPR011704

(FlyBase Curators et al., 2004-)

motor activity

inferred from sequence or structural similarity with EMBL:N39708

(FlyBase, 1992-)

Biological Process

CV term

establishment of mitotic spindle localization

References

traceable author statement

(Wang et al., 2003)

female germline ring canal formation

traceable author statement

(Deng and Lin, 2001)

fusome organization

traceable author statement

(Pepling et al., 1999, Deng and Lin, 2001)

germarium-derived oocyte fate determination

traceable author statement

(Deng and Lin, 2001)

germ-line cyst formation

traceable author statement

(Pepling et al., 1999, Deng and Lin, 2001)

intracellular mRNA localization

traceable author statement

(Cohen, 2002)

microtubule-based movement

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

non-traceable author statement

(Swiss-Prot Project Members, 1994.10.1)

oocyte nucleus migration involved in oocyte dorsal/ventral axis specification

traceable author statement

(Morris, 2003)

oogenesis

non-traceable author statement

Cellular Component

CV term

(Swiss-Prot Project Members, 1994.10.1)

References

cytoplasm

non-traceable author statement

cytoplasmic dynein complex

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

inferred from sequence or structural similarity with EMBL:N39708

(FlyBase, 1992-)

fusome

traceable author statement

(Pepling et al., 1999, Deng and Lin, 2001)

germline ring canal

traceable author statement

(Deng and Lin, 2001)

microtubule associated complex

non-traceable author statement

(Rasmusson, 1994.7.28)

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Protein-protein

Interacting group

Assay

References

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Dhc64C allele

Gene

References

Dhc64C^4-19

ctp

(Ghosh-Roy et al., 2004)

CycB

(Ji et al., 2002)

Khc

Klc

Dhc64C^6-6

Lis-1

(Swan et al., 1999)

Dhc64C^6-8

Dhc64C^6-10

CycB

(Ji et al., 2002)

Dhc64C^ek1

(Martin et al., 1997, Martin et al., 1999)

Khc

Klc

unspecified

Aplip1

(Horiuchi et al., 2005)

External Data

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0261797

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

38580

Orthologs

Genome-wide drosophilid orthologs

Dana\GF24711

Dere\GG14159

Dgri\GH15789

Dmoj\GI12568

Dpse\GA20400

Dvir\GJ15472

Dwil\GK17594

Dyak\GE20585

Curated drosophilid orthologs

Dhyd\Dhc6

(Kurek et al., 1998)

Dmir\CG7507

(Thornton, 2005.12.20)

Dpse\GA20400

y¹ v¹; P{TRiP.JF03177}attP2

OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs.

Stocks & Reagents

Stocks Listed in FlyBase ( 10 )

Bloomington

28749

23863

w^*; Dhc64C^4-19 P{FRT(w^hs)}2A/TM6B, Tb¹

5274

mwh¹ Dhc64C^4-19 jv¹ ca¹/TM6B, Tb⁺ ca¹

15139

y¹; P{SUPor-P}Dhc64C^KG08838 ry⁵⁰⁶/TM3, Sb¹ Ser¹

8747

mwh¹ Dhc64C^6-10 h¹ st¹ p^p e^s/TM6B, Tb¹

32015

mwh¹ Dhc64C^6-6 h¹ st¹ p^p ss¹ e^s/TM6B, Tb¹

Kyoto

108142

mwh¹ Dhc64C^4-19 jv¹ ca¹/TM6B, Tb¹ ca¹

111682

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{SUPor-P}Dhc64C^KG08838 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹

VDRC

v28054

w¹¹¹⁸; P{GD12258}v28054

v28053

w¹¹¹⁸; P{GD12258}v28053/TM3

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 24 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

MIP04978

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0261797

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

Antibody Information

polyclonal

(Erdelyi and Szabad, 1989)

Other Information

Discoverer

Etymology

"Laborc" named for a Hungarian family that vanished by the beginning of the 14th century.

Mutation is named after a Hungarian clan that vanished by the beginning of the 14th century but their names survived in the names of settlements.

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Dhc64C Laborc

(Belecz et al., 2001, Belecz et al., 2001)

Source for merge of: Dhc64C Su(Gl)77

(Iyadurai et al., 2008)

Additional comments

Allelism between Su(Gl)77, Su(Gl)102 and Su(Gl)160 has not been established. These are independent mutations with suppressing effects on Gl which all map between ru and h on the third chromosome.

(Lindsley and Zimm, 1992)

Other Comments

Dhc64C is required for the rapid transport of grk, bcd AND osk RNAs from nurse cells to the oocyte by a mechanism distinct from the general flow of cytoplasm from nurse cells to oocyte.

(Clark et al., 2007)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes spindle pole detachment when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

dsRNA has been synthesised for this gene and transfected into S2 cells. S2 cells treated with this dsRNA arrest in metaphase, require 50% more time to form a metaphase plate than untreated cells and exhibit centrosome detachment and spindle focusing defects.

(Griffis et al., 2007)

Dhc64C activity is required to maintain localisation of bcd at the anterior cortex.

(Weil et al., 2006)

Dhc64C is required to maintain mRNA at its apical localization, following transport, in the blastoderm embryo.

(Delanoue and Davis, 2005)

S2 cells transfected with dsRNA made from templates generated with primers directed against this gene causes detachment of centrosomes from the spindle poles, resulting in a slight increase of spindle size.

(Goshima et al., 2005)

Mitotic S2 cells treated with dsRNA made from templates generated with primers directed against this gene display a striking detachment of centrosomes from spindles and a loss of spindle pole focusing, resulting in an increase in pole-pole spacing. There is also an increase in spindle length and an elevation of the mitotic index.

(Morales-Mulia and Scholey, 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Dhc64C may be involved in the prevention of centrosome assembly in unfertilised eggs, and establishing harmony between the chromosome and centrosome cycles.

(Belecz et al., 2001)

Spindle pole movements in embryos are directed by a temporally coordinated balance of forces generated by three mitotic motors; cytoplasmic dynein, Klp61F and ncd. Dynein acts to move the poles apart throughout mitosis, and this activity is augmented by Klp61F after the fenestration of the nuclear envelope, which occurs at the onset of prometaphase. ncd generates forces that pull the poles together between interphase and metaphase, antagonising the activity of both cytoplasmic dynein and Klp61F and serving as a brake for spindle assembly.

(Sharp et al., 2000)

Mutations in Dhc64C disrupt fast organelle transport in both directions in axons.

Dhc64C function is required for the attachment and migration of centrosomes along the nuclear envelope during interphase/prophase and to maintain the attachment of centrosomes to mitotic spindle poles.

(Robinson et al., 1999)

Identification: PCR screen for Dynein heavy chain genes.

(Kurek et al., 1998)

Mutant analysis of Dhc64C reveals cytoplasmic dynein is required at two stages of oogenesis. The localisation of dynein in mitotic cysts suggests spindle orientation is mediated by the microtubule motor cytoplasmic dynein. Later in oogenesis dynein function is necessary for proper differentiation.

Early in oogenesis mutations disrupt spindle organisation in dividing cysts and block oocyte determination.

Cytoplasmic dynein encoded by Dhc64C is essential for Drosophila viability and for cell viability in several tissues.

(Gepner et al., 1996)

Clonal analysis suggests that cytoplasmic dynein mutations are cell lethal, and maternal supplies are sufficient for development to larval stages.

(Boylan and Hays, 1995)

Cytoplasmic dynein is required for the proper formation of the oocyte early in oogenesis. Later, during oocyte growth, dynein is required for the transport of materials from the nurse cells to the developing oocyte.

Dhc64C protein acts as a minus-end directed motor that promotes microtubule translocation in vitro.

Dhc64C encodes a cytoplasmic dynein heavy chain polypeptide.

(Li et al., 1994)

Dhc64C has been cloned and characterised.

(Martin et al., 1999, Martin et al., 1997)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

38580

UniProtKB/Swiss-Prot

•

P37276

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Dynein heavy chain, P-loop containing D4 domain (IPR024317)

Dynein heavy chain, coiled coil stalk (IPR024743)

ATPase, AAA+ type, core (IPR003593)

Dynein heavy chain (IPR004273)

ATPase, dynein-related, AAA domain (IPR011704)

Dynein heavy chain, domain-1 (IPR013594)

Dynein heavy chain, domain-2 (IPR013602)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0261797

DRSC - Results from RNAi screens.

•

FBgn0261797

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0261797

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

38580

modMine - Data generated by the modENCODE project.

•

FBgn0261797

OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs.

Synonyms & Secondary IDs ( 40 )

Reported As

Symbol Synonym

(Morris, 2000)

cDhc

(Brendza et al., 2002)

Cdhc

(Barton and Goldstein, 1996, )

cDhc64C

CG7507

(Fulton et al., 2001, Goldstein and Gunawardena, 2000, Przewloka et al., 2007, Goshima et al., 2007, Maresca and Salmon, 2009, Perkins et al., 2009, Hughes et al., 2008, Cammarato et al., 2011)

dhc

(Basto et al., 2004, Macdougall et al., 2003, Vaccari and Ephrussi, 2002, Wilkie and Davis, 2001, Fischer, 2000, Bullock et al., 2006, Bullock et al., 2006, Clark et al., 2007, Weil et al., 2006, Delanoue et al., 2007, Jaramillo et al., 2008, Zheng et al., 2008)

DHC

(Sharp et al., 2000, Cytrynbaum et al., 2003, Sharp et al., 2000, Silvanovich et al., 1998, Gindhart et al., 1998, Morales-Mulia and Scholey, 2005, Kim et al., 2007, Haghnia et al., 2007, Riggs et al., 2007, Rogers et al., 2008, Navarro et al., 2009, Ally et al., 2009, Loschi et al., 2009, Satoh et al., 2008)

Dhc46C

(Gao and Bogert, 2003, Sandmann et al., 2007)

Dhc64

dhc64C

(Macdougall et al., 2003, Rasmusson, 1994.7.28, Harris and Peifer, 2005, Haghnia et al., 2007, Johnson et al., 2009, Satoh et al., 2008)

Dhc64c

(Palacios and St. Johnston, 2002)

DHC64C

(Cox et al., 2001, Anderson et al., 2009, Loschi et al., 2009, Lorenzo et al., 2010, Loiseau et al., 2010)

Dynein

(Loschi et al., 2009)

Fs(3)Lab

(Szabad et al., 1995, )

Fs(3)Laborc

(Erdelyi and Szabad, 1989)

Fs(3)Sz18

HMW MAP

(Harte and Kankel, 1982, Iyadurai et al., 2008)

l(3)64Ca

Lab

(Foe et al., 1993)

Su(Gl)77

Name Synonym

Cytoplasmic dynein

(Welte, 2004)

cytoplasmic dynein

(de Heredia and Jansen, 2004, Gonczy, 2002, Rogers et al., 2000, Goshima and Vale, 2003, Maiato et al., 2004, Carter et al., 2008, Hays et al., 1994)

cytoplasmic dynein heavy chain

(Li, 1993, Schroer, 1994, Maiato et al., 2004, Zhang et al., 2009, Gaspar and Szabad, 2009)

Cytoplasmic dynein heavy chain

(Gao and Bogert, 2003)

cytoplasmic Dynein heavy chain