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General Information
Symbol
Dmel\LanB1
Species
D. melanogaster
Name
LanB1
Annotation Symbol
CG7123
Feature Type
FlyBase ID
FBgn0261800
Gene Model Status
Stock Availability
Gene Snapshot
LanB1 (LanB1) encodes a subunit of laminin, a heterotrimeric protein found in the extracellular matrix (ECM) and a major component of the basal lamina. Laminins interact with a variety of cell surface molecules such as integrin receptors and other secreted ECM components. It contributes to ECM organization, tissue adhesion, cell motility and developmental patterning. [Date last reviewed: 2019-09-26]
Also Known As
laminin, Laminin B1, laminin β, EP-600, LamB1
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:7,811,438..7,820,771 [+]
Recombination map
2-28
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
-
Summaries
Gene Group (FlyBase)
LAMININS -
Laminins are major structural components of the basement membrane. (Adapted from FBrf0230242.)
Protein Function (UniProtKB)
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components (By similarity). Required for Ndg localization to the basement membrane (PubMed:30260959).
(UniProt, P11046)
Summary (Interactive Fly)
extracellular matrix protein - required for normal accumulation of basement membrane components - involved in morphogenesis of gut, trachea, muscles and nervous system
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\LanB1 or the JBrowse view of Dmel\LanB1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0079490
6235
1788
FBtr0079491
5947
1788
Additional Transcript Data and Comments
Reported size (kB)
6.0 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0079113
198.3
1788
4.98
FBpp0079114
198.3
1788
4.98
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1788 aa isoforms: LanB1-PA, LanB1-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
1784 (aa); 220 (kD observed)
Comments
External Data
Subunit Structure (UniProtKB)
Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end.
(UniProt, P11046)
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. Domains VI and IV are globular.
(UniProt, P11046)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\LanB1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (17 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (14 terms)
Terms Based on Experimental Evidence (12 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from high throughput mutant phenotype
inferred from mutant phenotype
inferred from high throughput mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002576803
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000832288
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000832288
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000832288
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002576803
(assigned by GO_Central )
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN000832288
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
LanB1 transcripts are detected in 4-6hr embryos, peak at 10-12hr, and then decline. Later peaks occur in early larvae, and late pupae. Developmental increases in Laminin transcripts are associated with periods of morphogenesis and precede those of Cg25C transcripts. Laminin transcripts appear in newly formed mesoderm and are later expressed in fat body, in some glial cells and prominantly in hemocytes found distributed around many organs where basement membrane deposition occurs such as brain, nerve cord, sensory organs, and various internal organs. LanA transcripts are detected at a lower level than LanB1 and LanB2 transcripts.
LanB1 expression is first detected during germ band extension in the mesoderm and at lower levels in the ectoderm including the neurogenic region. At ~8hr, the level of LanB1 expression dramatically increases in both the somatic and visceral mesoderm and expression in these tissues continues through the rest of embryonic development. At 8hr, expression in the ectoderm also increases but mainly in the epidermis and not in ectodermal derivatives such as the salivary glands and the CNS. The only LanB1 expression in the CNS is found along the midline. The interface glia were also tentatively identified as a site of LanB1 expression. The patterns of expression of LanA, LanB1, and LanB2 are nearly identical.
LanB1 transcripts are expressed through most of embryogenesis with a peak in 6-9hr embryos.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
wing disc

Comment: basal side of columnar epithelium

imaginal disc epithelial cell of wing disc

Comment: basal surface 0-4 hrs APF to 20-28 hrs APF

wing disc

Comment: lacunae formed between the dorsal and ventral wing spaces 20-44 hours APF

wing vein

Comment: basal surface of longitudinal veins 32-44 hrs APF

mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Kc167 cell-line

Comment: in conditioned medium

Additional Descriptive Data
LanB1 was detected in the basement membranes of most embryonic tissues such as muscles and gut and is enriched at muscle attachment sites. It is prominently expressed in basement membranes of wing discs during larval and pupal development. In third instar larvae and early pupal stages, it is present on the basal side of the epithelium and colocalizes with integrins. It later localizes to the basal side of the lacuna formed after apposition of dorsal and ventral wing surfaces. It is later observed in developing longitudinal veins while integrins are found in the intervein regions.
In embryos, LanB1 protein is detected in fat body, in hemocytes and in basement membranes surrounding many tissues.
In dissected embryos between 10-13 hours of development, LanB1 is detected in basement membrane throughout the embryo. These include basement membranes covering the inside of the epidermis, developing muscles, gut, and internal glands and organs. LanA is also detected in the dorsal basement membrane overlying the developing CNS and in a prominent pair of mesodermal cells just dorsal to the nervous system. The most striking staining in the developing CNS is seen along the axon pathways, including the longitudinal pathways, the commissural pathways, and the peripheral nerve roots. The glia around the peripheral nerve roots also stain. Staining is also observed surrounding clusters of peripheral sensory organs and their support cells. All three Laminin subunits show similar patterns of staining.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\LanB1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of LanB1
Transgenic constructs containing regulatory region of LanB1
Deletions and Duplications ( 4 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
13 of 15
Yes
No
7 of 15
Yes
No
3 of 15
No
Yes
2 of 15
Yes
No
2 of 15
Yes
No
1 of 15
No
No
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
12 of 15
Yes
No
3 of 15
No
Yes
2 of 15
Yes
No
2 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
12 of 13
Yes
Yes
11 of 13
Yes
No
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (5)
8 of 12
Yes
Yes
7 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (10)
14 of 15
Yes
Yes
12 of 15
No
Yes
9 of 15
No
Yes
6 of 15
No
Yes
6 of 15
No
Yes
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
14 of 15
Yes
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900A7 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915004R )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00FI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X005Y )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G009B )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (9)
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end.
    (UniProt, P11046 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-28
    Cytogenetic map
    Sequence location
    2L:7,811,438..7,820,771 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    28C4-28D1
    Limits computationally determined from genome sequence between P{EP}EP2178 and P{PZ}mts02496; (determined by in situ hybridisation)
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    28D1-28D1
    (determined by in situ hybridisation)
    28D-28D
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (12)
    Genomic Clones (28)
    cDNA Clones (375)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Source for merge of: LanB1 EP-600
      Additional comments
      Other Comments
      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
      Chain-specific polyclonal antibodies reveal that LanB1 and LanB2 form a stable dimer before they are disulfide-bonded to each other. LanA associates with neither monomeric LanB1, monomeric LanB2 nor LanB1 LanB2 dimer without disulfide-bonding but only with disulfide-bonded LanB1 LanB2 dimer to form LanA LanB1 LanB2 trimers. Results demonstrate that the interchain disulfide-bonding between LanB1 and LanB2 is essential for LanA LanB1 LanB2 trimer formation.
      The three Laminin genes (LanA, LanB1 and LanB2) encode the three subunits of Drosophila laminin, which associate to form a cruciform molecule in which the carboxy-terminal ends are α helical and associate with one another and whose amino-terminal ends are free to form three short arms of the cross.
      The three Laminin genes (LanA, LanB1 and LanB2) encode the three subunits of laminin, a substrate adhesion molecule shown to be a potent promoter of neurite outgrowth.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 51 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      Other crossreferences
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      FlyMine - An integrated database for Drosophila genomics
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (28)
      Reported As
      Secondary FlyBase IDs
      • FBgn0002527
      • FBgn0259294
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (144)