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General Information
Symbol
Dmel\Msp300
Species
D. melanogaster
Name
Muscle-specific protein 300 kDa
Annotation Symbol
CG42768
Feature Type
FlyBase ID
FBgn0261836
Gene Model Status
Stock Availability
Gene Snapshot
Muscle-specific protein 300 kDa (Msp300) encodes a Nesprin-like protein that is required for proper positioning of muscle nuclei, mitochondria, and neuromuscular junction. [Date last reviewed: 2019-03-14]
Also Known As
Msp-300, nesprin, Muscle-specific protein 300, jf22
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:5,100,877..5,207,002 [+]
Recombination map
2-15
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Summaries
Gene Group (FlyBase)
KASH DOMAIN GENES -
The KASH domain consists of a C-terminal transmembrane domain followed by a 30 amino acid domain sequence. This domain targets the protein to the outer leaflet of the nuclear membrane by interacting with SUN domain proteins. KASH proteins are involved in mediating nuclear interactions such as with the cytoskeleton for localisation of the nucleus. (Adapted from FBrf0189793).
Summary (Interactive Fly)
cytoskeletal protein - transports mRNAs from the nucleus to postsynaptic sites during synaptic maturation - controls glutamate receptor density at the neuromuscular junction - promotes myonuclear spacing
Gene Model and Products
Number of Transcripts
11
Number of Unique Polypeptides
11

Please see the GBrowse view of Dmel\Msp300 or the JBrowse view of Dmel\Msp300 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0303383
25760
8382
FBtr0303385
39327
12345
FBtr0303384
30030
9606
FBtr0303386
2610
407
FBtr0303387
43121
13540
FBtr0303388
37913
11986
FBtr0303389
36339
11999
FBtr0332403
24228
7962
FBtr0332404
36093
11917
FBtr0332405
39216
12308
FBtr0332406
20943
6685
Additional Transcript Data and Comments
Reported size (kB)
~13 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0292445
958.4
8382
5.16
FBpp0292447
1405.1
12345
4.86
FBpp0292446
1063.7
9606
4.22
FBpp0292448
47.3
407
6.73
FBpp0292449
1507.8
13540
4.28
FBpp0292450
1366.0
11986
4.84
FBpp0292451
1367.6
11999
4.85
FBpp0304676
905.8
7962
4.79
FBpp0304677
1358.4
11917
4.84
FBpp0304678
1401.1
12308
4.86
FBpp0304679
749.1
6685
4.52
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
300 (kD)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Msp300 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (28 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
Biological Process (16 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:klar; FB:FBgn0001316
inferred from mutant phenotype
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
Cellular Component (8 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
colocalizes_with actin filament
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR012315
(assigned by InterPro )
inferred from sequence or structural similarity with UniProtKB:Q8NF91
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
pcr
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Msp300 transcripts are expressed throughout development except in pupae. They are most abundant in late embryos.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
In myotubes with developed sarcomeric architecture, the protein is localized mainly to the Z discs.
In third instar larvae, Msp300 is detected in the peripodial epithelium overlying the eye disc. In late pupal eye discs, perinuclear Msp300 is found in photoreceptor cells.
Msp300 protein is initially expressed in a small subset of muscle precursors in each segment at ventral, lateral, and dorsal locations and in visceral muscles. It is expressed by muscle precursors at muscle-ectoderm and muscle-muscle attachment sites. As development continues it becomes associated with the myofibrillar network. Migrating myotubes exhibit high Msp300 levels at their leading edge.
Marker for
Subcellular Localization
CV Term
Evidence
References
colocalizes_with actin filament
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Msp300 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 34 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Msp300
Transgenic constructs containing regulatory region of Msp300
Deletions and Duplications ( 22 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
egg chamber & actin filament | maternal effect | germ-line clone
follicle cell & nucleus | somatic clone
nurse cell & cytoplasm | maternal effect | germ-line clone
nurse cell & nucleus | maternal effect | germ-line clone
oocyte & nucleus | maternal effect | germ-line clone
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
5 of 15
Yes
Yes
4 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
5 of 15
Yes
Yes
4 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (5)
3 of 13
Yes
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (6)
2 of 12
Yes
Yes
2 of 12
Yes
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (15)
5 of 15
Yes
Yes
4 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (5)
5 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190002 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila persimilis
Drosophila persimilis
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150007 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0004 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0003 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0007 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 2 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map
2-15
Cytogenetic map
Sequence location
2L:5,100,877..5,207,002 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
25C6-25C6
Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338); Limits computationally determined from genome sequence between P{lacW}l(2)k11206k11206 and P{lacW}tkvk16713&P{lacW}l(2)k01302k01302
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
25C9-25C9
(determined by in situ hybridisation)
25C6-25C10
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (33)
Genomic Clones (42)
cDNA Clones (69)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: nesprin CG31649
    Source for identity of: Msp300 Msp-300
    Source for database merge of
    Source for merge of: Msp-300 CG18251 CG18252
    Source for merge of: nesprin BcDNA:GH18470
    Source for merge of: Msp-300 nesprin
    Source for merge of: CG14035 Msp-300
    Source for merge of: Msp-300 CG31916
    Source for merge of: nesprin BcDNA:AT10293
    Source for merge of: CG31916 BcDNA:LP10936
    Source for merge of: CG31916 BcDNA:GH02446
    Source for merge of: Msp-300 Bbbf1
    Additional comments
    Annotations CG14035 and CG33715 merged as CG42768 in release 5.28 of the genome annotation.
    Source for identity of nesprin CG31649 was sequence comparison ( date:021108 ).
    Isolation of the AF521598 cDNA suggests that "Msp-300" and "nesprin" may represent two parts of a single gene.
    Source for merge of nesprin BcDNA:AT10293 was shared cDNA (AT10293) ( date:030530 ).
    Source for merge of CG31916 BcDNA:LP10936 was a shared cDNA ( date:030728 ).
    Source for merge of CG31916 BcDNA:GH02446 was a shared cDNA ( date:030728 ).
    Source for merge of nesprin BcDNA:GH18470 was a shared cDNA ( date:030728 ).
    Other Comments
    The complete loss of Msp-300 function does not lead to defects in nuclear positioning in egg chambers.
    The KASH domain isoforms of Msp-300 have no essential function for nuclear migration during eye development.
    KASH forms of Msp-300 function in egg-laying.
    KASH forms of Msp-300 are not required for viability, nor for oogenesis.
    The KASH forms of Msp-300 do not play a redundant role with KASH forms of klar in nuclear positioning in the eye or the ovary.
    Analysis of egg chambers produced from Msp-300 germ-line clones shows that Msp-300 has a role in actin-dependent nuclear anchorage during nurse cell to oocyte cytoplasmic transport.
    Annotations CG31649 and CG33549 merged as CG33715 in release 4.2 of the genome annotation.
    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
    snmRNA:128 is derived from the ORF of Msp-300. snmRNA:765 is derived from the ORF of Msp-300.
    New annotation (CG31649) in release 3 of the genome annotation. New annotation (CG31916) in release 3 of the genome annotation.
    Examination of mutant embryos and the function of Msp-300 during embryonic muscle development suggests a functional link between LanA and Msp-300 in the development of the embryonic somatic musculature. Msp-300 displays significant similarity to vertebrate Dystrophin.
    MSP-300 decorates actin filaments and is expressed by muscle precursors at muscle-ectoderm and muscle-muscle attachment sites. As development continues it becomes associated with the muscle myofibrillar network. Migrating myotubes exhibit high MSP-300 levels at their leading edge, but in myotubes with developed sarcomeric architecture the protein is localised mainly to the Z discs.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 127 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (36)
    Reported As
    Symbol Synonym
    Bbbf1
    BcDNA:AT10293
    BcDNA:GH02446
    BcDNA:GH18470
    BcDNA:LP10936
    CG31916
    CG33549
    Spec25CD
    l(2)25Ch
    l(2)jf22
    Name Synonyms
    Box B-binding factor 1
    MUSCLE-SPECIFIC PROTEIN 300
    Muscle-specific protein 300
    Muscle-specific protein 300 kDa
    Muscle-specific protein, 300 kDa
    Muscle-specific protein-300
    lethal(2)25Ch
    repetitive ORF
    Secondary FlyBase IDs
    • FBgn0031685
    • FBgn0053715
    • FBgn0001926
    • FBgn0010070
    • FBgn0010270
    • FBgn0031686
    • FBgn0031687
    • FBgn0047188
    • FBgn0051649
    • FBgn0051916
    • FBgn0053549
    • FBgn0062498
    • FBgn0063615
    • FBgn0064983
    • FBgn0065020
    • FBgn0011271
    • FBgn0260952
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (158)