FB2019_05, released Oct 15, 2019

Gene: Dmel\Msp300

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General Information
Symbol
Dmel\Msp300
Species
D. melanogaster
Name
Muscle-specific protein 300 kDa
Annotation Symbol
CG42768
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261836
Gene Model Status
Current
Stock Availability
33 publicly available
Gene Snapshot
Muscle-specific protein 300 kDa (Msp300) encodes a Nesprin-like protein that is required for proper positioning of muscle nuclei, mitochondria, and neuromuscular junction. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As
Msp-300, nesprin, Muscle-specific protein 300, jf22
Key Links
Genomic Location
Cytogenetic map
25C6-25C10
Sequence location
2L:5,100,877..5,207,002 [+]
Recombination map
2-15
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
actin binding; protein binding; protein kinase binding
Predictions / Assertions
actin binding; actin filament binding
Summaries
Gene Group (FlyBase)
KASH DOMAIN GENES -
The KASH domain consists of a C-terminal transmembrane domain followed by a 30 amino acid domain sequence. This domain targets the protein to the outer leaflet of the nuclear membrane by interacting with SUN domain proteins. KASH proteins are involved in mediating nuclear interactions such as with the cytoskeleton for localisation of the nucleus. (Adapted from FBrf0189793).
Summary (Interactive Fly)
cytoskeletal protein - transports mRNAs from the nucleus to postsynaptic sites during synaptic maturation - controls glutamate receptor density at the neuromuscular junction - promotes myonuclear spacing
Gene Model and Products
Number of Transcripts
11
Number of Unique Polypeptides
11

Please see the GBrowse view of Dmel\Msp300 or the JBrowse view of Dmel\Msp300 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Sequence Ontology: Class of Gene
gene
gene_with_edited_transcript
(Rodriguez et al., 2012, Graveley et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Msp300-RB
FBtr0303383
NM_001201763
25760
8382
Msp300-RD
FBtr0303385
NM_001201765
39327
12345
Msp300-RE
FBtr0303384
NM_001201764
30030
9606
Msp300-RF
FBtr0303386
NM_001169414
2610
407
Msp300-RG
FBtr0303387
NM_001201766
43121
13540
Msp300-RH
FBtr0303388
NM_001201767
37913
11986
Msp300-RI
FBtr0303389
NM_001201768
36339
11999
Msp300-RJ
FBtr0332403
NM_001273159
24228
7962
Msp300-RK
FBtr0332404
NM_001273160
36093
11917
Msp300-RL
FBtr0332405
NM_001273161
39216
12308
Msp300-RM
FBtr0332406
NM_001273162
20943
6685
Additional Transcript Data and Comments
Reported size (kB)
~13 (northern blot)
(Volk, 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Msp300-PB
FBpp0292445
958.4
8382
5.16
NP_001188692
ADV36942
Msp300-PD
FBpp0292447
1405.1
12345
4.86
NP_001188694
ADV36944
Msp300-PE
FBpp0292446
1063.7
9606
4.22
NP_001188693
ADV36943
Msp300-PF
FBpp0292448
47.3
407
6.73
NP_001162885
ACZ94176
Msp300-PG
FBpp0292449
1507.8
13540
4.28
NP_001188695
ADV36945
Msp300-PH
FBpp0292450
1366.0
11986
4.84
NP_001188696
ADV36946
Msp300-PI
FBpp0292451
1367.6
11999
4.85
NP_001188697
ADV36947
Msp300-PJ
FBpp0304676
905.8
7962
4.79
NP_001260088
AGB92624
Msp300-PK
FBpp0304677
1358.4
11917
4.84
NP_001260089
AGB92625
Msp300-PL
FBpp0304678
1401.1
12308
4.86
NP_001260090
AGB92626
Msp300-PM
FBpp0304679
749.1
6685
4.52
NP_001260091
AGB92627
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
320, 230 (kD)
(Rosenberg-Hasson et al., 1996)
300 (kD)
(Volk, 1992)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Msp300 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (28 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
actin binding
inferred from direct assay
(Volk, 1992)
protein binding
inferred from physical interaction with FLYBASE:klar; FB:FBgn0001316
(Elhanany-Tamir et al., 2012)
protein kinase binding
inferred from physical interaction with FLYBASE:sls; FB:FBgn0086906
(Elhanany-Tamir et al., 2012)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
actin binding
inferred from sequence or structural similarity
(Roper et al., 2002)
actin filament binding
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (16 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
actin filament organization
inferred from mutant phenotype
(Yu et al., 2006)
cell migration
inferred from mutant phenotype
(Junion et al., 2007)
cytoplasmic transport, nurse cell to oocyte
inferred from mutant phenotype
(Yu et al., 2006)
endoplasmic reticulum localization
inferred from mutant phenotype
(Elhanany-Tamir et al., 2012)
female germline ring canal stabilization
inferred from mutant phenotype
(Yu et al., 2006)
flight
inferred from mutant phenotype
(Elhanany-Tamir et al., 2012)
larval visceral muscle development
inferred from mutant phenotype
(Elhanany-Tamir et al., 2012)
locomotion
inferred from mutant phenotype
(Elhanany-Tamir et al., 2012)
microtubule anchoring
inferred from genetic interaction with FLYBASE:klar; FB:FBgn0001316
(Elhanany-Tamir et al., 2012)
mitochondrion localization
inferred from mutant phenotype
(Elhanany-Tamir et al., 2012)
nuclear migration
inferred from mutant phenotype
(Yu et al., 2006)
nucleus localization
inferred from genetic interaction with FLYBASE:klar; FB:FBgn0001316
(Elhanany-Tamir et al., 2012)
inferred from mutant phenotype
(Tan et al., 2018)
nucleus organization
inferred from genetic interaction with FLYBASE:klar; FB:FBgn0001316
(Elhanany-Tamir et al., 2012)
skeletal muscle tissue development
inferred from mutant phenotype
(Junion et al., 2007)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
cytoskeletal anchoring at nuclear membrane
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
(Gaudet et al., 2011)
mesoderm development
non-traceable author statement
(Furlong et al., 2001)
nuclear migration
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
(Gaudet et al., 2011)
nucleus localization
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (8 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
colocalizes_with actin filament
inferred from direct assay
(Huelsmann et al., 2013)
cytoplasm
inferred from direct assay
(Yu et al., 2006)
nuclear envelope
inferred from direct assay
(Yu et al., 2006)
nuclear outer membrane
inferred from direct assay
(Morel et al., 2014, Elhanany-Tamir et al., 2012)
perinuclear region of cytoplasm
inferred from direct assay
(Montana and Littleton, 2006)
Z disc
inferred from direct assay
(Morel et al., 2014, Elhanany-Tamir et al., 2012, Montana and Littleton, 2006)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
(Gaudet et al., 2011)
integral component of membrane
inferred from electronic annotation with InterPro:IPR012315
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
meiotic nuclear membrane microtubule tethering complex
inferred from sequence or structural similarity with UniProtKB:Q8NF91
(Morel et al., 2014)
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
(Gaudet et al., 2011)
nuclear outer membrane
inferred from biological aspect of ancestor with PANTHER:PTN002934743
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
(Volk, 1992)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 6
yolk
(Fisher et al., 2012)
yolk nucleus
(Fisher et al., 2012)
embryonic stage 7 -- 8
yolk nucleus
(Fisher et al., 2012)
embryonic stage 9 -- 10
yolk nucleus
(Fisher et al., 2012)
embryonic stage 11 -- 12
yolk nucleus
(Fisher et al., 2012)
posterior spiracle primordium

Comment: reported as posterior spiracle specific anlage

(Fisher et al., 2012)
embryonic stage 13 -- 16
amnioserosa
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic ventral epidermis
(Fisher et al., 2012)
embryonic/larval oenocyte
(Fisher et al., 2012)
embryonic/larval posterior spiracle
(Fisher et al., 2012)
dorsal pharyngeal muscle
(Fisher et al., 2012)
embryonic/larval muscle system
(Fisher et al., 2012)
embryonic/larval somatic muscle
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Volk, 1992)
adult stage
(Volk, 1992)
pcr
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism
(Xie and Fischer, 2008)
third instar larval stage
eye disc
(Xie and Fischer, 2008)
Additional Descriptive Data
Msp300 transcripts are expressed throughout development except in pupae. They are most abundant in late embryos.
(Volk, 1992)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
muscle attachment site
(Volk, 1992)
embryonic/larval visceral muscle
(Volk, 1992)
embryonic/larval muscle system
(Volk, 1992)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
somatic muscle
• Z disc
(Elhanany-Tamir et al., 2012)
peripodial epithelium
(Xie and Fischer, 2008)
pupal stage
photoreceptor cell
(Xie and Fischer, 2008)
oogenesis
oocyte
(Technau and Roth, 2008)
follicle cell | punctate
(Technau and Roth, 2008)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010, Dorus et al., 2006)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
In myotubes with developed sarcomeric architecture, the protein is localized mainly to the Z discs.
(Technau and Roth, 2008, Volk, 1992)
In third instar larvae, Msp300 is detected in the peripodial epithelium overlying the eye disc. In late pupal eye discs, perinuclear Msp300 is found in photoreceptor cells.
(Xie and Fischer, 2008)
Msp300 protein is initially expressed in a small subset of muscle precursors in each segment at ventral, lateral, and dorsal locations and in visceral muscles. It is expressed by muscle precursors at muscle-ectoderm and muscle-muscle attachment sites. As development continues it becomes associated with the myofibrillar network. Migrating myotubes exhibit high Msp300 levels at their leading edge.
(Volk, 1992)
Marker for
 
mesoderm
(Zmojdzian and Jagla, 2013)
Subcellular Localization
CV Term
Evidence
References
colocalizes_with actin filament
inferred from direct assay
(Huelsmann et al., 2013)
cytoplasm
inferred from direct assay
(Yu et al., 2006)
nuclear envelope
inferred from direct assay
(Yu et al., 2006)
nuclear outer membrane
inferred from direct assay
(Morel et al., 2014, Elhanany-Tamir et al., 2012)
perinuclear region of cytoplasm
inferred from direct assay
(Montana and Littleton, 2006)
Z disc
inferred from direct assay
(Morel et al., 2014, Elhanany-Tamir et al., 2012, Montana and Littleton, 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Msp300 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 34 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Msp300
Transgenic constructs containing regulatory region of Msp300
Deletions and Duplications ( 22 )
Not disrupted in
Df(2L)cl1
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl7
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl-h1
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl-h2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl-h3
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)cl-h4
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)Msp300-3
(Technau and Roth, 2008)
Df(2L)Msp300-5
(Technau and Roth, 2008)
Df(2L)Msp300-compl
(Technau and Roth, 2008)
Df(2L)sc19-6
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-7
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-8
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)tkv2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Partially disrupted in
Df(2L)Msp300-3
(Technau and Roth, 2008)
Df(2L)Msp300-5
(Technau and Roth, 2008)
Disrupted in
Df(2L)Exel6011
(Ryder, 2004.4.26)
Df(2L)Msp300-compl
(Technau and Roth, 2008)
Df(2L)sc19-1
(Yu et al., 2006, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-4
(Rosenberg-Hasson et al., 1996, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-5
(Rosenberg-Hasson et al., 1996, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-12
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)tkv3
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Duplicated in
Dp(2;1)B19
(Xie and Fischer, 2008)
Tp(2;1)B19
(Yu et al., 2006)
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
lethal - all die before end of embryonic stage | recessive
lethal - all die before end of larval stage
lethal - all die during embryonic stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
partially lethal - majority die
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile
male fertile
Other Phenotypes
Allele
decreased fecundity | female | germline clone
flightless
flightless, with Scer\GAL4Mef2.PR
locomotor behavior defective | larval stage
locomotor behavior defective | third instar larval stage
locomotor behavior defective | third instar larval stage, with Scer\GAL4how-24B
neurophysiology defective | third instar larval stage
size defective (with Msp3003prime)
size defective (with Msp300ΔKASH)
some die during embryonic stage | recessive
some die during larval stage
Phenotype manifest in
Allele
A1-7 segment border muscle, with Scer\GAL4how-24B
cytoplasmic microtubule | third instar larval stage
dorsal appendage | germline clone | maternal effect
egg (with Msp3003prime)
egg (with Msp300ΔKASH)
egg | germline clone | maternal effect
egg chamber & actin filament | maternal effect | germ-line clone
embryonic/larval hypodermal muscle | third instar larval stage
embryonic/larval hypodermal muscle | third instar larval stage, with Scer\GAL4how-24B
embryonic/larval muscle system | third instar larval stage
embryonic/larval somatic muscle
follicle cell & nucleus | somatic clone
nucleus | third instar larval stage
nucleus | third instar larval stage, with Scer\GAL4how-24B
nurse cell & cytoplasm | maternal effect | germ-line clone
nurse cell & nucleus | maternal effect | germ-line clone
oocyte & nucleus | maternal effect | germ-line clone
ring canal | germline clone | maternal effect
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\SYNE1
5 of 15
Yes
Yes
Hsap\SYNE2
4 of 15
Yes
No
Hsap\CLMN
3 of 15
Yes
No
Hsap\SYNE3
2 of 15
Yes
No
Hsap\EVPLL
1 of 15
No
No
Hsap\GAS2L1
1 of 15
No
No
Hsap\GAS2L2
1 of 15
No
No
Hsap\MACF1
1 of 15
No
No
Hsap\PLEC
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Syne2
5 of 15
Yes
Yes
Mmus\Syne1
4 of 15
Yes
No
Mmus\Clmn
2 of 15
Yes
No
Mmus\Dst
1 of 15
No
No
Mmus\Macf1
1 of 15
No
No
Mmus\Plec
1 of 15
No
No
Mmus\Syne3
1 of 15
Yes
No
Rattus norvegicus (Norway rat) (5)
Rnor\Clmn
3 of 13
Yes
Yes
Rnor\Gas2l1
1 of 13
No
No
Rnor\Gas2l2
1 of 13
No
No
Rnor\Plec
1 of 13
No
No
Rnor\Syne3
1 of 13
Yes
No
Xenopus tropicalis (Western clawed frog) (6)
Xtro\syne1
2 of 12
Yes
Yes
Xtro\syne2
2 of 12
Yes
Yes
Xtro\dst
1 of 12
No
Yes
Xtro\gas2l2
1 of 12
No
No
Xtro\macf1
1 of 12
No
Yes
Xtro\plec
1 of 12
No
No
Danio rerio (Zebrafish) (15)
Drer\syne1a
5 of 15
Yes
Yes
Drer\syne1b
4 of 15
Yes
No
Drer\syne2a
3 of 15
Yes
No
Drer\syne2b
2 of 15
Yes
No
Drer\clmn
1 of 15
No
Yes
Drer\dspa
1 of 15
No
No
Drer\dst
1 of 15
No
No
Drer\eppk1
1 of 15
No
No
Drer\gas2b
1 of 15
No
No
Drer\macf1a
1 of 15
No
No
Drer\pleca
1 of 15
No
No
Drer\si:ch211-137a8.2
1 of 15
Yes
No
Drer\syne3
1 of 15
No
Yes
Drer\wu:fi04e12
1 of 15
No
No
Drer\zgc:100997
1 of 15
Yes
No
Caenorhabditis elegans (Nematode, roundworm) (5)
Cele\anc-1
5 of 15
Yes
Yes
Cele\csp-2
1 of 15
No
No
Cele\D2096.11
1 of 15
Yes
No
Cele\M116.5
1 of 15
No
No
Cele\vab-10
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190002 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Msp300
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000257
Drosophila simulans
Dsim\GD23326
Drosophila sechellia
Dsec\GM18533
Drosophila sechellia
Dsec\GM18531
Drosophila erecta
Dere\GG25055
Drosophila yakuba
Dyak\GE11346
Drosophila ananassae
Dana\GF14378
Drosophila persimilis
Dper\GL18808
Drosophila persimilis
Dper\GL20674
Drosophila persimilis
Dper\GL18807
Drosophila willistoni
Dwil\GK24496
Drosophila virilis
Dvir\GJ11158
Drosophila mojavensis
Dmoj\GI21363
Drosophila grimshawi
Dgri\GH10368
Drosophila grimshawi
Dgri\GH10367
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150007 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013253
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_06711
Mayetiola destructor
Hessian fly
Mdes\Mdes003521
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007898
Anopheles darlingi
American malaria mosquito
Adar\ADAC009952
Anopheles gambiae
Malaria mosquito
Agam\AGAP009554
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ015331
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0004 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA010471
Danaus plexippus
Monarch butterfly
Dple\DPOGS202792-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL009277
Apis florea
Little honeybee
Aflo\2173975828
Apis mellifera
Western honey bee
Amel\GB40007
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP13547
Bombus terrestris
Buff-tailed bumblebee
Bter\15212070
Linepithema humile
Argentine ant
Lhum\LH21009
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002ff1
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG003481
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10067
Tribolium castaneum
Red flour beetle
Tcas\TC034351
Pediculus humanus
Human body louse
Phum\PHUM347080
Rhodnius prolixus
Kissing bug
Rpro\RPRC010799
Cimex lectularius
Bed bug
Clec\CLEC006038
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI081140
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006305
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_14720
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_14719
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_16295
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0003 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR013014
Stegodyphus mimosarum
African social velvet spider
Smim\KFM65653.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM65651.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM65652.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM65657.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur20g00190
Tetranychus urticae
Two-spotted spider mite
Turt\tetur20g00160
Tetranychus urticae
Two-spotted spider mite
Turt\tetur20g00150
Tetranychus urticae
Two-spotted spider mite
Turt\tetur20g00170
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_209558
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0007 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Syne1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Syne1L
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_015521gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Syne1L_3
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_007994gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Syne1L_2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_006984gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000011799
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000010861
Gallus gallus
Domestic chicken
Ggal\SYNE1
Gallus gallus
Domestic chicken
Ggal\SYNE2
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000011071
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG5984
1 of 10
cher
1 of 10
jbug
1 of 10
shot
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 2 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
SYNE1; spectrin repeat containing nuclear envelope protein 1
5 of 15
608441
SYNE2; spectrin repeat containing nuclear envelope protein 2
4 of 15
608442
CLMN; calmin
3 of 15
611121
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Msp300
      suppressible
      GluRIIA
      (Morel et al., 2014)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      klar
      enhanceable
      Msp300
      (Elhanany-Tamir et al., 2012, Xie and Fischer, 2008)
      kud
      suppressible
      Msp300
      (Ding et al., 2017)
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map
      2-15
      Cytogenetic map
      25C6-25C10
      Sequence location
      2L:5,100,877..5,207,002 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      25C6-25C10
      Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      25C-25C
      (Zhang, 2002.3.27)
      24C-24D
      (Volk, 1993.1.12)
      25C9-25C9
      (determined by in situ hybridisation)
      (Rosenberg-Hasson et al., 1996, Volk, 1992)
      25C6-25C10
      (determined by in situ hybridisation)
      (BDGP Project Members, 1994-1999)
      Experimentally Determined Recombination Data
      Location
      2-15
      (FlyBase, 2016)
      2-17.3
      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (33)
      Genomic Clones (42)
      cDNA Clones (69)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        polyclonal
        (Rosenberg-Hasson et al., 1996, Volk, 1992)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for identity of: nesprin CG31649
        (FlyBase, 1996-)
        Source for identity of: Msp300 Msp-300
        (FlyBase, 2009-)
        Source for database merge of
        Source for merge of: Msp-300 CG18251 CG18252
        (Bayraktaroglu, 2000.4.3)
        Source for merge of: nesprin BcDNA:GH18470
        (FlyBase, 1996-)
        Source for merge of: Msp-300 nesprin
        (FlyBase Genome Annotators, 2005)
        Source for merge of: CG14035 Msp-300
        (FlyBase Genome Annotators, 2010)
        Source for merge of: Msp-300 CG31916
        (FlyBase Genome Annotators, 2005)
        Source for merge of: nesprin BcDNA:AT10293
        (FlyBase, 1996-)
        Source for merge of: CG31916 BcDNA:LP10936
        (FlyBase, 1996-)
        Source for merge of: CG31916 BcDNA:GH02446
        (FlyBase, 1996-)
        Source for merge of: Msp-300 Bbbf1
        (FlyBase, 1996-)
        Additional comments
        Annotations CG14035 and CG33715 merged as CG42768 in release 5.28 of the genome annotation.
        (FlyBase Genome Annotators, 2010)
        Source for identity of nesprin CG31649 was sequence comparison ( date:021108 ).
        (FlyBase Genome Annotators, 2005, FlyBase, 1996-)
        Isolation of the AF521598 cDNA suggests that "Msp-300" and "nesprin" may represent two parts of a single gene.
        (Zhang et al., 2002)
        Source for merge of nesprin BcDNA:AT10293 was shared cDNA (AT10293) ( date:030530 ).
        (FlyBase, 1996-)
        Source for merge of CG31916 BcDNA:LP10936 was a shared cDNA ( date:030728 ).
        (FlyBase, 1996-)
        Source for merge of CG31916 BcDNA:GH02446 was a shared cDNA ( date:030728 ).
        (FlyBase, 1996-)
        Source for merge of nesprin BcDNA:GH18470 was a shared cDNA ( date:030728 ).
        (FlyBase, 1996-)
        Other Comments
        The complete loss of Msp-300 function does not lead to defects in nuclear positioning in egg chambers.
        (Technau and Roth, 2008)
        The KASH domain isoforms of Msp-300 have no essential function for nuclear migration during eye development.
        (Technau and Roth, 2008)
        KASH forms of Msp-300 function in egg-laying.
        (Xie and Fischer, 2008)
        KASH forms of Msp-300 are not required for viability, nor for oogenesis.
        (Xie and Fischer, 2008)
        The KASH forms of Msp-300 do not play a redundant role with KASH forms of klar in nuclear positioning in the eye or the ovary.
        (Xie and Fischer, 2008)
        Analysis of egg chambers produced from Msp-300 germ-line clones shows that Msp-300 has a role in actin-dependent nuclear anchorage during nurse cell to oocyte cytoplasmic transport.
        (Yu et al., 2006)
        Annotations CG31649 and CG33549 merged as CG33715 in release 4.2 of the genome annotation.
        (FlyBase Genome Annotators, 2005)
        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
        (Kiger et al., 2003)
        snmRNA:128 is derived from the ORF of Msp-300. snmRNA:765 is derived from the ORF of Msp-300.
        (Yuan et al., 2003)
        New annotation (CG31649) in release 3 of the genome annotation. New annotation (CG31916) in release 3 of the genome annotation.
        (FlyBase Genome Annotators, 2002-2003)
        Examination of mutant embryos and the function of Msp-300 during embryonic muscle development suggests a functional link between LanA and Msp-300 in the development of the embryonic somatic musculature. Msp-300 displays significant similarity to vertebrate Dystrophin.
        (Rosenberg-Hasson et al., 1996)
        MSP-300 decorates actin filaments and is expressed by muscle precursors at muscle-ectoderm and muscle-muscle attachment sites. As development continues it becomes associated with the muscle myofibrillar network. Migrating myotubes exhibit high MSP-300 levels at their leading edge, but in myotubes with developed sarcomeric architecture the protein is localised mainly to the Z discs.
        (Volk, 1992)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 127 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        FlyMine - An integrated database for Drosophila genomics
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (36)
        Reported As
        Symbol Synonym
        BBF-1
        (Abel, 1994.3.25)
        Bbbf1
        BcDNA:AT10293
        BcDNA:GH02446
        BcDNA:GH18470
        BcDNA:LP10936
        CG14035
        (Hallier et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Markstein et al., 2008, Technau and Roth, 2008, Zhang et al., 2007, Sambandan et al., 2006)
        CG18251
        (Haye et al., 2014)
        CG18252
        (Bolshakov et al., 2002, Goldstein and Gunawardena, 2000, Goldstein and Gunawardena, 2000)
        CG31649
        (Yuan et al., 2003)
        CG31916
        CG33549
        CG33715
        (Chen et al., 2012, Christis and Munro, 2012, Laflamme et al., 2012, Cammarato et al., 2011, Rees et al., 2011, Zhang et al., 2011, Anholt and Williams, 2010, Zhang et al., 2010, Keleman et al., 2009.8.5)
        CG42768
        (Hosono et al., 2015, FlyBase Genome Annotators, 2010, Ni et al., 2010.12.1)
        MSP-300
        (Johnson et al., 2013, Elhanany-Tamir et al., 2012, Fabian and Brill, 2012, Nir et al., 2012, Elhanany and Volk, 2008, Montana and Littleton, 2006, Yu et al., 2006, Starr and Fischer, 2005, Arbeitman et al., 2002, Roper et al., 2002, Zhang et al., 2002, Rosenberg-Hasson et al., 1996, Volk, 1993.1.12)
        MSP300
        (Packard et al., 2015, Abmayr et al., 1995)
        Msp-300
        (Al-Ramahi et al., 2018, Potikanond et al., 2018, Angrisani et al., 2015, Chang et al., 2015, Kim et al., 2015, Ashwal-Fluss et al., 2014, Ma and Weake, 2014, Montgomery et al., 2014, Morel et al., 2014, Chintapalli et al., 2013, Chow et al., 2013, de Celis et al., 2013.9.11, Huelsmann et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Winbush et al., 2012, Friedman et al., 2011, Wasbrough et al., 2010, Markstein et al., 2008, Technau and Roth, 2008, Xie and Fischer, 2008, Bakal et al., 2007, Junion et al., 2007, Xie and Fischer, 2007, Zhang et al., 2007, Dorus et al., 2006)
        Msp300
        (De et al., 2019, Ditzler et al., 2019, Mannix et al., 2019, Bernard et al., 2018, Reuveny et al., 2018, Wang et al., 2018, Ding et al., 2017, Hu et al., 2017.6.13, Nie et al., 2017, Clandinin and Owens, 2016-, Kuleesha et al., 2016, Gene Disruption Project members, 2015-, Wójtowicz et al., 2015, Schönbauer et al., 2011, Zmojdzian et al., 2008, Junion et al., 2007)
        Spec25CD
        dNesp1
        (Packard et al., 2015)
        jf22
        (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
        l(2)25Ch
        l(2)jf22
        msp-300
        (Tan et al., 2018, Jayo et al., 2016, Hallier et al., 2015, Haye et al., 2014, McDermott et al., 2014, Xie and Fischer, 2007, Yu et al., 2006, Starr and Han, 2003)
        msp300
        (Xiong and Zhou, 2013, Jia and Huan, 2010, Kracklauer et al., 2010)
        Name Synonyms
        Box B-binding factor 1
        MUSCLE-SPECIFIC PROTEIN 300
        (Hull et al., 2015)
        Msp-300
        (Westholm et al., 2014)
        Muscle-specific protein 300
        (Cammarato et al., 2011)
        Muscle-specific protein 300 kDa
        Muscle-specific protein, 300 kDa
        (Kwong et al., 2015)
        Muscle-specific protein-300
        (Xie and Fischer, 2008)
        Nesprin
        (Zhang et al., 2002)
        Nesprin1
        (Packard et al., 2015)
        lethal(2)25Ch
        nesprin
        (Lewis, 1998, FlyBase, 1996-)
        repetitive ORF
        (Zhang et al., 2002)
        Secondary FlyBase IDs
        • FBgn0031685
        • FBgn0053715
        • FBgn0001926
        • FBgn0010070
        • FBgn0010270
        • FBgn0031686
        • FBgn0031687
        • FBgn0047188
        • FBgn0051649
        • FBgn0051916
        • FBgn0053549
        • FBgn0062498
        • FBgn0063615
        • FBgn0064983
        • FBgn0065020
        • FBgn0011271
        • FBgn0260952
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (160)