FlyBase Gene Report: Dmel\Msp300

General Information

Symbol

Dmel\Msp300

Species

D. melanogaster

Name

Muscle-specific protein 300 kDa

Annotation Symbol

CG42768

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261836

Gene Model Status

Stock Availability

27 publicly available

Gene Summary

Muscle-specific protein 300 kDa (Msp300) encodes a Nesprin-like protein that is required for proper positioning of muscle nuclei, mitochondria, and neuromuscular junction. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Msp-300, nesprin, jf22, Muscle-specific protein 300

Key Links

Genomic Location

Cytogenetic map

Sequence location

2L:5,100,877..5,207,002 [+]

Recombination map

2-15

RefSeq locus

NT_033779 REGION:5100877..5207002

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (26 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables actin binding

inferred from direct assay

(Volk, 1992)

enables protein binding

inferred from physical interaction with FLYBASE:klar; FB:FBgn0001316

(Elhanany-Tamir et al., 2012)

enables protein kinase binding

inferred from physical interaction with FLYBASE:sls; FB:FBgn0086906

(Elhanany-Tamir et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN002934743

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (15 terms)

Terms Based on Experimental Evidence (14 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from mutant phenotype

(Yu et al., 2006)

involved_in cell migration

inferred from mutant phenotype

(Junion et al., 2007)

involved_in cytoplasmic transport, nurse cell to oocyte

inferred from mutant phenotype

(Yu et al., 2006)

involved_in endoplasmic reticulum localization

inferred from mutant phenotype

(Elhanany-Tamir et al., 2012)

involved_in female germline ring canal stabilization

inferred from mutant phenotype

(Yu et al., 2006)

involved_in flight

inferred from mutant phenotype

(Elhanany-Tamir et al., 2012)

involved_in larval visceral muscle development

inferred from mutant phenotype

(Elhanany-Tamir et al., 2012)

involved_in locomotion

inferred from mutant phenotype

(Elhanany-Tamir et al., 2012)

involved_in microtubule anchoring

inferred from genetic interaction with FLYBASE:klar; FB:FBgn0001316

(Elhanany-Tamir et al., 2012)

involved_in mitochondrion localization

inferred from mutant phenotype

(Elhanany-Tamir et al., 2012)

involved_in nuclear migration

inferred from mutant phenotype

(Yu et al., 2006)

involved_in nucleus localization

inferred from genetic interaction with FLYBASE:klar; FB:FBgn0001316

(Elhanany-Tamir et al., 2012)

inferred from mutant phenotype

(Tan et al., 2018)

involved_in nucleus organization

inferred from genetic interaction with FLYBASE:klar; FB:FBgn0001316

(Elhanany-Tamir et al., 2012)

involved_in somatic muscle development

inferred from mutant phenotype

(Junion et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in mesoderm development

non-traceable author statement

(Furlong et al., 2001)

involved_in nuclear migration

inferred from biological aspect of ancestor with PANTHER:PTN002934743

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

colocalizes_with actin filament

inferred from direct assay

(Huelsmann et al., 2013)

located_in cytoplasm

inferred from direct assay

(Yu et al., 2006)

located_in nuclear outer membrane

inferred from direct assay

(Morel et al., 2014, Elhanany-Tamir et al., 2012)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Montana and Littleton, 2006)

located_in Z disc

inferred from direct assay

(Morel et al., 2014, Elhanany-Tamir et al., 2012, Montana and Littleton, 2006)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002934743

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in integral component of membrane

inferred from electronic annotation with InterPro:IPR012315

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

part_of meiotic nuclear membrane microtubule tethering complex

inferred from biological aspect of ancestor with PANTHER:PTN002934743

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:Q8NF91

(Morel et al., 2014)

is_active_in nuclear outer membrane

inferred from biological aspect of ancestor with PANTHER:PTN002934743

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

KASH DOMAIN GENES

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Muscle-specific protein 300 kDa (Msp300) encodes a Nesprin-like protein that is required for proper positioning of muscle nuclei, mitochondria, and neuromuscular junction. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

KASH DOMAIN GENES -: The KASH domain consists of a C-terminal transmembrane domain followed by a 30 amino acid domain sequence. This domain targets the protein to the outer leaflet of the nuclear membrane by interacting with SUN domain proteins. KASH proteins are involved in mediating nuclear interactions such as with the cytoskeleton for localisation of the nucleus. (Adapted from FBrf0189793).

Summary (Interactive Fly)

cytoskeletal protein - transports mRNAs from the nucleus to postsynaptic sites during synaptic maturation - controls glutamate receptor density at the neuromuscular junction - promotes myonuclear spacing

Interactive Fly

Gene Model and Products

Number of Transcripts

11

Number of Unique Polypeptides

11

Please see the JBrowse view of Dmel\Msp300 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0303383

25760

8382

FBtr0303385

39327

12345

FBtr0303384

30030

9606

FBtr0303386

2610

407

FBtr0303387

43121

13540

FBtr0303388

37913

11986

FBtr0303389

36339

11999

FBtr0332403

24228

7962

FBtr0332404

36093

11917

FBtr0332405

39216

12308

FBtr0332406

20943

6685

Additional Transcript Data and Comments

Reported size (kB)

~13 (northern blot)

(Volk, 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0292445

958.4

8382

5.16

FBpp0292447

1405.1

12345

4.86

FBpp0292446

1063.7

9606

4.22

FBpp0292448

47.3

407

6.73

FBpp0292449

1507.8

13540

4.28

FBpp0292450

1366.0

11986

4.84

FBpp0292451

1367.6

11999

4.85

FBpp0304676

905.8

7962

4.79

FBpp0304677

1358.4

11917

4.84

FBpp0304678

1401.1

12308

4.86

FBpp0304679

749.1

6685

4.52

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

320, 230 (kD)

(Rosenberg-Hasson et al., 1996)

300 (kD)

(Volk, 1992)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Msp300 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

(Volk, 1992)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

(Fisher et al., 2012)

(Fisher et al., 2012)

embryonic stage 7 -- 8

(Fisher et al., 2012)

embryonic stage 9 -- 10

(Fisher et al., 2012)

embryonic stage 11 -- 12

(Fisher et al., 2012)

posterior spiracle primordium

Comment: reported as posterior spiracle specific anlage

(Fisher et al., 2012)

embryonic stage 13 -- 16

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

embryonic/larval oenocyte

(Fisher et al., 2012)

embryonic/larval posterior spiracle

(Fisher et al., 2012)

dorsal pharyngeal muscle

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval somatic muscle

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Volk, 1992)

(Volk, 1992)

pcr

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Xie and Fischer, 2008)

third instar larval stage

(Xie and Fischer, 2008)

Additional Descriptive Data

Msp300 transcripts are expressed throughout development except in pupae. They are most abundant in late embryos.

(Volk, 1992)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

muscle attachment site

(Volk, 1992)

embryonic/larval visceral muscle

(Volk, 1992)

embryonic/larval muscle system

(Volk, 1992)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

• Z disc

(Elhanany-Tamir et al., 2012)

peripodial epithelium

(Xie and Fischer, 2008)

photoreceptor cell

(Xie and Fischer, 2008)

(Technau and Roth, 2008)

follicle cell | punctate

(Technau and Roth, 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

(Wasbrough et al., 2010, Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

(Cammarato et al., 2011)

Additional Descriptive Data

In myotubes with developed sarcomeric architecture, the protein is localized mainly to the Z discs.

(Technau and Roth, 2008, Volk, 1992)

In third instar larvae, Msp300 is detected in the peripodial epithelium overlying the eye disc. In late pupal eye discs, perinuclear Msp300 is found in photoreceptor cells.

(Xie and Fischer, 2008)

Msp300 protein is initially expressed in a small subset of muscle precursors in each segment at ventral, lateral, and dorsal locations and in visceral muscles. It is expressed by muscle precursors at muscle-ectoderm and muscle-muscle attachment sites. As development continues it becomes associated with the myofibrillar network. Migrating myotubes exhibit high Msp300 levels at their leading edge.

(Volk, 1992)

Marker for

(Zmojdzian and Jagla, 2013)

Subcellular Localization

CV Term

Evidence

References

colocalizes_with actin filament

inferred from direct assay

(Huelsmann et al., 2013)

located_in cytoplasm

inferred from direct assay

(Yu et al., 2006)

located_in nuclear outer membrane

inferred from direct assay

(Morel et al., 2014, Elhanany-Tamir et al., 2012)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Montana and Littleton, 2006)

located_in Z disc

inferred from direct assay

(Morel et al., 2014, Elhanany-Tamir et al., 2012, Montana and Littleton, 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Msp300 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Msp300

View exonic expression by tissue for Dmel\Msp300

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261836

DRscDB

3771968/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0261836

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0261836

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 35 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Msp300

Transgenic constructs containing regulatory region of Msp300

Aberrations (Deficiencies and Duplications) ( 22 )

Inferred from experimentation ( 22 )

Gene not disrupted in

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Technau and Roth, 2008)

(Technau and Roth, 2008)

Df(2L)Msp300-compl

(Technau and Roth, 2008)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

Gene partially disrupted in

(Technau and Roth, 2008)

(Technau and Roth, 2008)

Gene duplicated in

(Xie and Fischer, 2008)

(Yu et al., 2006)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

Msp300^MI00111

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Msp300^GD8201

lethal - all die before end of larval stage

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Msp300^GD8201

lethal - all die during embryonic stage | recessive

Msp300^sz75

partially lethal - majority die

Msp300^sz75

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Sterility

Allele

Msp300^sz75

Msp300^ΔKASH

Other Phenotypes

Allele

abnormal locomotor behavior | larval stage

Msp300^3prime

abnormal locomotor behavior | third instar larval stage

Msp300^ΔKASH

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^how-24B

Msp300^GD10317

abnormal neurophysiology | third instar larval stage

Msp300^ΔKASH

abnormal size (with Msp300^3prime)

Msp300^ΔKASH

abnormal size (with Msp300^ΔKASH)

Msp300^3prime

abnormal size | third instar larval stage, with Scer\GAL4^Cg.PA

Msp300^HMS00368

decreased fecundity | female | germline clone

Msp300^sz75

Msp300^3prime

flightless, with Scer\GAL4^Mef2.PR

Msp300^GD10317

some die during embryonic stage | recessive

Msp300^sz75

some die during larval stage

Phenotype manifest in

Allele

A1-7 segment border muscle, with Scer\GAL4^how-24B

Msp300^NIG.31916R

cytoplasmic microtubule | third instar larval stage

Msp300^ΔKASH

dorsal appendage | germline clone | maternal effect

Msp300^sz75

egg (with Msp300^3prime)

Msp300^ΔKASH

egg (with Msp300^ΔKASH)

Msp300^3prime

egg | germline clone | maternal effect

Msp300^sz75

egg chamber & actin filament | maternal effect | germ-line clone

Msp300^sz75

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

Msp300^HMS00368

embryonic/larval hypodermal muscle | third instar larval stage

embryonic/larval hypodermal muscle | third instar larval stage, with Scer\GAL4^how-24B

Msp300^GD10317

embryonic/larval muscle system | third instar larval stage

embryonic/larval somatic muscle

Msp300^sz75

fat cell | third instar larval stage, with Scer\GAL4^Cg.PA

Msp300^HMS00368

follicle cell & nucleus | somatic clone

Msp300^sz75

microtubule | third instar larval stage, with Scer\GAL4^Cg.PA

Msp300^HMS00368

microtubule organizing center | third instar larval stage, with Scer\GAL4^Cg.PA

Msp300^HMS00368

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

Msp300^HMS00368

nucleus | third instar larval stage

nucleus | third instar larval stage, with Scer\GAL4^Cg.PA

Msp300^HMS00368

nucleus | third instar larval stage, with Scer\GAL4^how-24B

Msp300^GD10317

nurse cell & cytoplasm | maternal effect | germ-line clone

Msp300^sz75

nurse cell & nucleus | maternal effect | germ-line clone

Msp300^sz75

oocyte & nucleus | maternal effect | germ-line clone

Msp300^sz75

ring canal | germline clone | maternal effect

Msp300^sz75

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

5 of 15

Yes

Yes

4 of 15

No

Yes

3 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

3 of 15

Yes

Yes

3 of 15

Yes

Yes

3 of 15

Yes

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

Rattus norvegicus (Norway rat) (14)

3 of 13

Yes

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

Yes

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (14)

3 of 12

Yes

Yes

2 of 12

No

Yes

2 of 12

No

Yes

1 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Danio rerio (Zebrafish) (23)

3 of 15

Yes

Yes

3 of 15

Yes

Yes

2 of 15

No

Yes

2 of 15

No

Yes

2 of 15

No

Yes

Drer\zgc:100997

2 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Drer\si:ch211-137a8.2

1 of 15

No

Yes

Drer\wu:fi04e12

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (5)

4 of 15

Yes

Yes

2 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190002 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00000257

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila persimilis

Drosophila persimilis

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150007 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_06711

Mayetiola destructor

Hessian fly

Mdes\Mdes003521

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007898

Anopheles darlingi

American malaria mosquito

Adar\ADAC009952

Anopheles gambiae

Malaria mosquito

Agam\AGAP009554

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015331

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0004 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA010471

Danaus plexippus

Monarch butterfly

Dple\DPOGS202792-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL009277

Apis florea

Little honeybee

Aflo\2173975828

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002ff1

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003481

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM347080

Rhodnius prolixus

Kissing bug

Rpro\RPRC010799

Cimex lectularius

Bed bug

Clec\CLEC006038

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI081140

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006305

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_14720

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_14719

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_16295

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0003 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR013014

Stegodyphus mimosarum

African social velvet spider

Smim\KFM65653.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM65651.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM65652.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM65657.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur20g00190

Tetranychus urticae

Two-spotted spider mite

Turt\tetur20g00160

Tetranychus urticae

Two-spotted spider mite

Turt\tetur20g00150

Tetranychus urticae

Two-spotted spider mite

Turt\tetur20g00170

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_209558

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0007 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_015521gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Syne1L_3

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_007994gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Syne1L_2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_006984gn

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000011799

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000010861

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000011071

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Emery-Dreifuss muscular dystrophy, LINC-complex-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

model of Emery-Dreifuss muscular dystrophy

CEA

(Wang et al., 2015)

Potential Models Based on Orthology ( 4 )

Human Ortholog

Disease

Evidence

References

SYNE1; spectrin repeat containing nuclear envelope protein 1

model of arthrogryposis multiplex congenita-3

IEA

(FlyBase, 2019-)

model of autosomal dominant Emery-Dreifuss muscular dystrophy 4

IEA

(FlyBase, 2019-)

model of autosomal recessive spinocerebellar ataxia 8

IEA

(FlyBase, 2019-)

SYNE2; spectrin repeat containing nuclear envelope protein 2

model of autosomal dominant Emery-Dreifuss muscular dystrophy 5

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

model of Emery-Dreifuss muscular dystrophy

is exacerbated by shot^SF20

(Wang et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SYNE1; spectrin repeat containing nuclear envelope protein 1

5 of 15

SYNE2; spectrin repeat containing nuclear envelope protein 2

4 of 15

EMERY-DREIFUSS MUSCULAR DYSTROPHY 5, AUTOSOMAL DOMINANT; EDMD5

autosomal dominant Emery-Dreifuss muscular dystrophy 5

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti bait coimmunoprecipitation, western blot

(Reuveny et al., 2018)

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Ding et al., 2017, Elhanany-Tamir et al., 2012)

anti tag coimmunoprecipitation, anti tag western blot

(Ding et al., 2017)

(Hu et al., 2017.6.13)

anti bait coimmunoprecipitation, western blot

(Elhanany-Tamir et al., 2012)

RNA-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, quantitative reverse transcription pcr, fluorescence correlation spectroscopy, anti bait coimmunoprecipitation

(Titlow et al., 2020, McDermott et al., 2014)

anti bait coimmunoprecipitation, primer specific pcr

(Packard et al., 2015)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Morel et al., 2014)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Elhanany-Tamir et al., 2012, Xie and Fischer, 2008)

suppressible

(Ding et al., 2017)

suppressible

(Sun et al., 2019)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261836

MIST (protein-protein) - An integrated Molecular Interaction Database

Msp300

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2L

Recombination map

2-15

Cytogenetic map

Sequence location

2L:5,100,877..5,207,002 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25C6-25C10

Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

25C-25C

(Zhang, 2002.3.27)

24C-24D

(Volk, 1993.1.12)

25C9-25C9

(determined by in situ hybridisation)

(Rosenberg-Hasson et al., 1996, Volk, 1992)

25C6-25C10

(determined by in situ hybridisation)

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-15

(FlyBase, 2016)

2-17.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (27)

26781

w^*; TI{TI}Msp300^ΔKASH

43447

w^*; P{GSV1}Msp300^EP-787

92580

w^*; P{UAS-EGFP.KASH-Msp300}attP2

92203

w¹¹¹⁸; P{UAS-MYC.Msp300.KASH}attP40

63765

w¹¹¹⁸; PBac{IT.GAL4}Msp300^0734-G4

43857

y¹ w^*; Mi{MIC}Msp300^MI02921

v105694

P{KK112013}VIE-260B

v107183

P{KK112156}VIE-260B

v109023

P{KK113643}VIE-260B

v17346

w¹¹¹⁸; P{GD8201}v17346

v50192

w¹¹¹⁸ P{GD10317}v50192

v25906

w¹¹¹⁸; P{GD10406}v25906

More stocks available...

Genomic Clones (42)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (69)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261836

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261836

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

3771968

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Rosenberg-Hasson et al., 1996, Volk, 1992)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: nesprin CG31649

(FlyBase, 1996-)

Source for identity of: Msp300 Msp-300

(FlyBase, 2009-)

Source for database merge of

Source for merge of: Msp-300 CG18251 CG18252

(Bayraktaroglu, 2000.4.3)

Source for merge of: nesprin BcDNA:GH18470

(FlyBase, 1996-)

Source for merge of: Msp-300 nesprin

(FlyBase Genome Annotators, 2005)

Source for merge of: CG14035 Msp-300

(FlyBase Genome Annotators, 2010)

Source for merge of: Msp-300 CG31916

(FlyBase Genome Annotators, 2005)

Source for merge of: nesprin BcDNA:AT10293

(FlyBase, 1996-)

Source for merge of: CG31916 BcDNA:LP10936

(FlyBase, 1996-)

Source for merge of: CG31916 BcDNA:GH02446

(FlyBase, 1996-)

Source for merge of: Msp-300 Bbbf1

(FlyBase, 1996-)

Additional comments

Annotations CG14035 and CG33715 merged as CG42768 in release 5.28 of the genome annotation.

(FlyBase Genome Annotators, 2010)

Source for identity of nesprin CG31649 was sequence comparison ( date:021108 ).

(FlyBase Genome Annotators, 2005, FlyBase, 1996-)

Isolation of the AF521598 cDNA suggests that "Msp-300" and "nesprin" may represent two parts of a single gene.

(Zhang et al., 2002)

Source for merge of nesprin BcDNA:AT10293 was shared cDNA (AT10293) ( date:030530 ).

(FlyBase, 1996-)

Source for merge of CG31916 BcDNA:LP10936 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Source for merge of CG31916 BcDNA:GH02446 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Source for merge of nesprin BcDNA:GH18470 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Other Comments

The complete loss of Msp-300 function does not lead to defects in nuclear positioning in egg chambers.

(Technau and Roth, 2008)

The KASH domain isoforms of Msp-300 have no essential function for nuclear migration during eye development.

(Technau and Roth, 2008)

KASH forms of Msp-300 function in egg-laying.

(Xie and Fischer, 2008)

KASH forms of Msp-300 are not required for viability, nor for oogenesis.

(Xie and Fischer, 2008)

The KASH forms of Msp-300 do not play a redundant role with KASH forms of klar in nuclear positioning in the eye or the ovary.

(Xie and Fischer, 2008)

Analysis of egg chambers produced from Msp-300 germ-line clones shows that Msp-300 has a role in actin-dependent nuclear anchorage during nurse cell to oocyte cytoplasmic transport.

(Yu et al., 2006)

Annotations CG31649 and CG33549 merged as CG33715 in release 4.2 of the genome annotation.

(FlyBase Genome Annotators, 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

snmRNA:128 is derived from the ORF of Msp-300. snmRNA:765 is derived from the ORF of Msp-300.

(Yuan et al., 2003)

New annotation (CG31649) in release 3 of the genome annotation. New annotation (CG31916) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Examination of mutant embryos and the function of Msp-300 during embryonic muscle development suggests a functional link between LanA and Msp-300 in the development of the embryonic somatic musculature. Msp-300 displays significant similarity to vertebrate Dystrophin.

(Rosenberg-Hasson et al., 1996)

MSP-300 decorates actin filaments and is expressed by muscle precursors at muscle-ectoderm and muscle-muscle attachment sites. As development continues it becomes associated with the muscle myofibrillar network. Migrating myotubes exhibit high MSP-300 levels at their leading edge, but in myotubes with developed sarcomeric architecture the protein is localised mainly to the Z discs.

(Volk, 1992)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 129 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

3771968

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261836

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261836

DRscDB

3771968/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0261836

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0261836

FlyMine - An integrated database for Drosophila genomics

FBgn0261836

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

3771968

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034351

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG42768

MARRVEL_MODEL

3771968

modMine - A data warehouse for the modENCODE project

FBgn0261836

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261836

DRSC - Results frm RNAi screens

FBgn0261836

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

Msp300

Synonyms and Secondary IDs (36)

Reported As

Symbol Synonym

BBF-1

(Abel, 1994.3.25)

Bbbf1

BcDNA:AT10293

BcDNA:GH02446

BcDNA:GH18470

BcDNA:LP10936

CG14035

(Hallier et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Markstein et al., 2008, Technau and Roth, 2008, Zhang et al., 2007, Sambandan et al., 2006)

CG18251

(Haye et al., 2014)

CG18252

(Bolshakov et al., 2002, Goldstein and Gunawardena, 2000, Goldstein and Gunawardena, 2000)

CG31649

(Yuan et al., 2003)

CG31916

CG33549

CG33715

(Chen et al., 2012, Christis and Munro, 2012, Laflamme et al., 2012, Cammarato et al., 2011, Rees et al., 2011, Zhang et al., 2011, Anholt and Williams, 2010, Zhang et al., 2010, Keleman et al., 2009.8.5)

CG42768

(Hosono et al., 2015, FlyBase Genome Annotators, 2010, Ni et al., 2010.12.1)

MSP-300

(Azevedo and Baylies, 2020, Johnson et al., 2013, Elhanany-Tamir et al., 2012, Fabian and Brill, 2012, Nir et al., 2012, Elhanany and Volk, 2008, Montana and Littleton, 2006, Yu et al., 2006, Starr and Fischer, 2005, Arbeitman et al., 2002, Roper et al., 2002, Zhang et al., 2002, Rosenberg-Hasson et al., 1996, Volk, 1993.1.12)

MSP300

(Zhou et al., 2019, Packard et al., 2015, Abmayr et al., 1995)

Spec25CD

dNesp1

(Packard et al., 2015)

jf22

(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)

l(2)25Ch

l(2)jf22

msp-300

(Tan et al., 2018, Jayo et al., 2016, Hallier et al., 2015, Haye et al., 2014, McDermott et al., 2014, Xie and Fischer, 2007, Yu et al., 2006, Starr and Han, 2003)

msp300

(Deng et al., 2021, Xiong and Zhou, 2013, Jia and Huan, 2010, Kracklauer et al., 2010)

Name Synonyms

Box B-binding factor 1

MUSCLE-SPECIFIC PROTEIN 300

(Hull et al., 2015)

Msp-300

(Westholm et al., 2014)

Muscle-specific protein 300

(Cammarato et al., 2011)

Muscle-specific protein 300 kDa

(Azevedo and Baylies, 2020)

Muscle-specific protein, 300 kDa

(Kwong et al., 2015)

Muscle-specific protein-300

(Xie and Fischer, 2008)

Nesprin

(Zhang et al., 2002)

Nesprin1

(Packard et al., 2015)

lethal(2)25Ch

nesprin

(Lewis, 1998, FlyBase, 1996-)

repetitive ORF

(Zhang et al., 2002)

Secondary FlyBase IDs

FBgn0031685
FBgn0053715
FBgn0001926
FBgn0010070
FBgn0010270
FBgn0031686
FBgn0031687
FBgn0047188
FBgn0051649
FBgn0051916
FBgn0053549
FBgn0062498
FBgn0063615
FBgn0064983
FBgn0065020
FBgn0011271
FBgn0260952

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (174)

Report Sections