Msp-300, nesprin, jf22, Muscle-specific protein 300
cytoskeletal protein - transports mRNAs from the nucleus to postsynaptic sites during synaptic maturation - controls glutamate receptor density at the neuromuscular junction - promotes myonuclear spacing
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
~13 (northern blot)
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Msp300 using the Feature Mapper tool.
In myotubes with developed sarcomeric architecture, the protein is localized mainly to the Z discs.
Msp300 protein is initially expressed in a small subset of muscle precursors in each segment at ventral, lateral, and dorsal locations and in visceral muscles. It is expressed by muscle precursors at muscle-ectoderm and muscle-muscle attachment sites. As development continues it becomes associated with the myofibrillar network. Migrating myotubes exhibit high Msp300 levels at their leading edge.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Msp300 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: Msp-300 CG18251 CG18252
Source for merge of: nesprin BcDNA:GH18470
Source for merge of: Msp-300 nesprin
Source for merge of: CG14035 Msp-300
Source for merge of: Msp-300 CG31916
Source for merge of: nesprin BcDNA:AT10293
Source for merge of: CG31916 BcDNA:LP10936
Source for merge of: CG31916 BcDNA:GH02446
Source for merge of: Msp-300 Bbbf1
Annotations CG14035 and CG33715 merged as CG42768 in release 5.28 of the genome annotation.
Source for identity of nesprin CG31649 was sequence comparison ( date:021108 ).
Isolation of the AF521598 cDNA suggests that "Msp-300" and "nesprin" may represent two parts of a single gene.
Source for merge of nesprin BcDNA:AT10293 was shared cDNA (AT10293) ( date:030530 ).
Source for merge of CG31916 BcDNA:LP10936 was a shared cDNA ( date:030728 ).
Source for merge of CG31916 BcDNA:GH02446 was a shared cDNA ( date:030728 ).
Source for merge of nesprin BcDNA:GH18470 was a shared cDNA ( date:030728 ).
The complete loss of Msp-300 function does not lead to defects in nuclear positioning in egg chambers.
The KASH domain isoforms of Msp-300 have no essential function for nuclear migration during eye development.
Annotations CG31649 and CG33549 merged as CG33715 in release 4.2 of the genome annotation.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
New annotation (CG31649) in release 3 of the genome annotation. New annotation (CG31916) in release 3 of the genome annotation.
Examination of mutant embryos and the function of Msp-300 during embryonic muscle development suggests a functional link between LanA and Msp-300 in the development of the embryonic somatic musculature. Msp-300 displays significant similarity to vertebrate Dystrophin.
MSP-300 decorates actin filaments and is expressed by muscle precursors at muscle-ectoderm and muscle-muscle attachment sites. As development continues it becomes associated with the muscle myofibrillar network. Migrating myotubes exhibit high MSP-300 levels at their leading edge, but in myotubes with developed sarcomeric architecture the protein is localised mainly to the Z discs.