General Information
Symbol
Dmel\sdt
Species
D. melanogaster
Name
stardust
Annotation Symbol
CG32717
Feature Type
FlyBase ID
FBgn0261873
Gene Model Status
Stock Availability
Enzyme Name (EC)
Guanylate kinase (2.7.4.8)
Gene Snapshot
Stardust is membrane-associated guanylate kinase (MAGUK) protein involved in the maintenance of apico-basal cell polarity and the organization of zonula adherens. [Date last reviewed: 2016-06-30]
Also Known As
Std, Pals1
Genomic Location
Cytogenetic map
Sequence location
X:8,178,549..8,240,474 [+]
Recombination map
1-22
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Experimental Evidence
Predictions / Assertions
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + GMP = ADP + GDP (2.7.4.8)
Summaries
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
sdt: stardust
Embryonic lethal. Hypoderm almost totally absent; only small remains of cuticle found. sdt+ function not required in female germline (Wieschaus and Noell, 1986, Roux's Arch. Dev. Biol. 195: 63-73).
Gene Model and Products
Number of Transcripts
14
Number of Unique Polypeptides
12

Please see the GBrowse view of Dmel\sdt or the JBrowse view of Dmel\sdt for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.50
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.55
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0089978
6828
1292
FBtr0089975
3838
879
FBtr0089974
6490
1367
FBtr0100375
4482
859
FBtr0100376
8462
2020
FBtr0111034
4017
934
FBtr0308217
5214
1269
FBtr0308218
6406
1527
FBtr0308219
3790
879
FBtr0308220
7745
1941
FBtr0333720
9152
1901
FBtr0333721
5650
1292
FBtr0340141
7220
1606
FBtr0340142
6968
1173
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0088916
139.4
1292
7.04
FBpp0088913
95.3
879
7.06
FBpp0088912
148.3
1367
7.06
FBpp0099784
93.9
859
7.20
FBpp0111740
217.8
2020
6.79
FBpp0110333
102.9
934
7.20
FBpp0300537
136.9
1269
7.08
FBpp0300538
165.4
1527
6.99
FBpp0300539
95.3
879
7.06
FBpp0300540
208.7
1941
6.83
FBpp0305869
205.8
1901
6.78
FBpp0305870
139.4
1292
7.04
FBpp0309127
174.5
1606
6.91
FBpp0309128
129.1
1173
6.94
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1292 aa isoforms: sdt-PB, sdt-PN
879 aa isoforms: sdt-PD, sdt-PK
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sdt using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (19 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:par-6; FB:FBgn0026192
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with MGI:MGI:1927339
Biological Process (11 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
Cellular Component (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from physical interaction with FLYBASE:Patj; FB:FBgn0067864
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
non-traceable author statement
traceable author statement
traceable author statement
traceable author statement
Expression Data
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from physical interaction with FLYBASE:Patj; FB:FBgn0067864
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\sdt in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 46 )
For All Classical and Insertion Alleles Show
 
Allele of sdt
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    Yes
      0
      Yes
        0
        Yes
          0
          --
            0
            Yes
            Other relevant insertions
            miscellaneous insertions
            Name
            Expression Data
            Transgenic Constructs ( 29 )
            For All Alleles Carried on Transgenic Constructs Show
            Transgenic constructs containing/affecting coding region of sdt
            Allele of sdt
            Mutagen
            Associated Transgenic Construct
            Stocks
            Transgenic constructs containing regulatory region of sdt
            Deletions and Duplications ( 9 )
            Summary of Phenotypes
            For more details about a specific phenotype click on the relevant allele symbol.
            Lethality
            Allele
            Other Phenotypes
            Allele
            Phenotype manifest in
            Allele
            photoreceptor cell & actin filament & pupa | somatic clone
            photoreceptor cell & adherens junction & pupa | somatic clone
            zonula adherens & photoreceptor cell & pupa
            Orthologs
            Human Orthologs (via DIOPT v7.1)
            Homo sapiens (Human) (8)
            Species\Gene Symbol
            Score
            Best Score
            Best Reverse Score
            Alignment
            Complementation?
            Transgene?
            11 of 15
            Yes
            Yes
            2 of 15
            No
            No
             
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            Model Organism Orthologs (via DIOPT v7.1)
            Mus musculus (laboratory mouse) (9)
            Species\Gene Symbol
            Score
            Best Score
            Best Reverse Score
            Alignment
            Complementation?
            Transgene?
            11 of 15
            Yes
            Yes
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            1 of 15
            No
            No
            1 of 15
            No
            No
            Rattus norvegicus (Norway rat) (8)
            9 of 13
            Yes
            Yes
            2 of 13
            No
            No
            2 of 13
            No
            No
            2 of 13
            No
            No
            2 of 13
            No
            No
            1 of 13
            No
            No
            1 of 13
            No
            No
            1 of 13
            No
            No
            Xenopus tropicalis (Western clawed frog) (6)
            7 of 12
            Yes
            Yes
            2 of 12
            No
            No
            1 of 12
            No
            No
            1 of 12
            No
            No
            1 of 12
            No
            No
            1 of 12
            No
            No
            Danio rerio (Zebrafish) (17)
            9 of 15
            Yes
            Yes
            6 of 15
            No
            Yes
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            2 of 15
            No
            No
            1 of 15
            No
            No
            1 of 15
            No
            No
            1 of 15
            No
            No
            1 of 15
            No
            No
            1 of 15
            No
            No
            1 of 15
            No
            No
            1 of 15
            No
            No
            Caenorhabditis elegans (Nematode, roundworm) (7)
            10 of 15
            Yes
            Yes
            2 of 15
            No
            No
            2 of 15
            No
            No
            1 of 15
            No
            Yes
            1 of 15
            No
            No
            1 of 15
            No
            No
            1 of 15
            No
            Yes
            Arabidopsis thaliana (thale-cress) (2)
            2 of 9
            Yes
            No
            1 of 9
            No
            No
            Saccharomyces cerevisiae (Brewer's yeast) (1)
            2 of 15
            Yes
            No
            Schizosaccharomyces pombe (Fission yeast) (1)
            1 of 12
            Yes
            No
            Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919017I )
            Organism
            Common Name
            Gene
            AAA Syntenic Ortholog
            Multiple Dmel Genes in this Orthologous Group
            Drosophila melanogaster
            fruit fly
            Drosophila suzukii
            Spotted wing Drosophila
            Drosophila simulans
            Drosophila sechellia
            Drosophila erecta
            Drosophila yakuba
            Drosophila ananassae
            Drosophila pseudoobscura pseudoobscura
            Drosophila persimilis
            Drosophila willistoni
            Drosophila virilis
            Drosophila mojavensis
            Drosophila grimshawi
            Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500P0 )
            Organism
            Common Name
            Gene
            Multiple Dmel Genes in this Orthologous Group
            Musca domestica
            House fly
            Glossina morsitans
            Tsetse fly
            Lucilia cuprina
            Australian sheep blowfly
            Mayetiola destructor
            Hessian fly
            Aedes aegypti
            Yellow fever mosquito
            Anopheles darlingi
            American malaria mosquito
            Anopheles gambiae
            Malaria mosquito
            Culex quinquefasciatus
            Southern house mosquito
            Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03BG )
            Organism
            Common Name
            Gene
            Multiple Dmel Genes in this Orthologous Group
            Bombyx mori
            Silkmoth
            Danaus plexippus
            Monarch butterfly
            Heliconius melpomene
            Postman butterfly
            Apis florea
            Little honeybee
            Apis mellifera
            Western honey bee
            Bombus impatiens
            Common eastern bumble bee
            Bombus impatiens
            Common eastern bumble bee
            Bombus terrestris
            Buff-tailed bumblebee
            Linepithema humile
            Argentine ant
            Megachile rotundata
            Alfalfa leafcutting bee
            Nasonia vitripennis
            Parasitic wasp
            Dendroctonus ponderosae
            Mountain pine beetle
            Tribolium castaneum
            Red flour beetle
            Pediculus humanus
            Human body louse
            Cimex lectularius
            Bed bug
            Acyrthosiphon pisum
            Pea aphid
            Zootermopsis nevadensis
            Nevada dampwood termite
            Zootermopsis nevadensis
            Nevada dampwood termite
            Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X038C )
            Organism
            Common Name
            Gene
            Multiple Dmel Genes in this Orthologous Group
            Strigamia maritima
            European centipede
            Ixodes scapularis
            Black-legged tick
            Stegodyphus mimosarum
            African social velvet spider
            Stegodyphus mimosarum
            African social velvet spider
            Tetranychus urticae
            Two-spotted spider mite
            Daphnia pulex
            Water flea
            Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G065I )
            Organism
            Common Name
            Gene
            Multiple Dmel Genes in this Orthologous Group
            Strongylocentrotus purpuratus
            Purple sea urchin
            Strongylocentrotus purpuratus
            Purple sea urchin
            Strongylocentrotus purpuratus
            Purple sea urchin
            Strongylocentrotus purpuratus
            Purple sea urchin
            Strongylocentrotus purpuratus
            Purple sea urchin
            Strongylocentrotus purpuratus
            Purple sea urchin
            Strongylocentrotus purpuratus
            Purple sea urchin
            Ciona intestinalis
            Vase tunicate
            Ciona intestinalis
            Vase tunicate
            Ciona intestinalis
            Vase tunicate
            Ciona intestinalis
            Vase tunicate
            Gallus gallus
            Domestic chicken
            Gallus gallus
            Domestic chicken
            Gallus gallus
            Domestic chicken
            Gallus gallus
            Domestic chicken
            Gallus gallus
            Domestic chicken
            Gallus gallus
            Domestic chicken
            Gallus gallus
            Domestic chicken
            Gallus gallus
            Domestic chicken
            Human Disease Model Data
            FlyBase Human Disease Model Reports
            Alleles Reported to Model Human Disease (Disease Ontology)
            Download
            Models ( 1 )
            Allele
            Disease
            Evidence
            References
            model of  cancer
            Interactions ( 0 )
            Allele
            Disease
            Interaction
            References
            Comments ( 0 )
             
            Human Orthologs (via DIOPT v7.1)
            Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
            Homo sapiens (Human)
            Gene name
            Score
            OMIM
            OMIM Phenotype
            Complementation?
            Transgene?
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser

            Please look at the Interaction Group reports for full details of the physical interactions
            protein-protein
            Interacting group
            Assay
            References
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement

            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            External Data
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            Pathways
            Gene Group - Pathway Membership (FlyBase)
            External Data
            Linkouts
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            X
            Recombination map
            1-22
            Cytogenetic map
            Sequence location
            X:8,178,549..8,240,474 [+]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            7D17-7D22
            Limits computationally determined from genome sequence between P{EP}CG1583EP1516&P{EP}CG1583EP325 and P{EP}sdtEP1143
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (26)
            Genomic Clones (26)
            cDNA Clones (103)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequences
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of
            Source for identity of: sdt CG1617 CG15341
            Source for database merge of
            Source for merge of: CG12658 sdt
            Source for merge of: sdt CG12657
            Source for merge of: sdt anon- EST:fe2E6
            Source for merge of: sdt CG15340
            Source for merge of: sdt BP1063
            Additional comments
            The release 5.2 annotation CG32717 (which corresponds to sdt) contains sequences corresponding to the release 2 annotation CG12657 (the transcript containing these sequences that was deleted in release 5.1 due to lack of evidence has been reinstated in release 5.2 due to new supporting evidence).
            Annotations CG32717, CG15342, CG32716 and CG12657 merged as a single annotation (CG32717, which corresponds to sdt) in release 3.2 of the genome annotation.
            Annotations CG12658, CG15340 and CG1617 merged as CG32717 in release 3 of the genome annotation.
            "l(1)G0463" may affect "sdt".
            Source for identity of sdt CG1617 CG15341 was sequence comparison ( date:011219 ).
            Other Comments
            RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
            sdt is required for the regionalization of apical membrane into stalk membranes and rhabdomeres.
            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
            sdt provides an essential component of the Subapical complex.
            sdt is a partner of crb in the control of epithelial cell polarity in the developing embryo.
            sdt interacts with crb to control polarity of epithelia but not neuroblasts.
            Loss of cell polarity in the epidermal primordium of sdt mutant embryos is associated with a failure to establish the zonulae adherentes. These junctions fail to develop when an altered polarity is induced by the overexpression of crb. crb and sdt have different functions during the formation of the zonula adherens.
            Embryos mutant for arm and sdt baz double mutant embryos have very similar early phenotypes with respect to zonula adherens formation. Results suggest that early stages in the assembly of the zonula adherens are critical for the stability of the polarised blastoderm epithelium.
            crb and sdt are required for the third step in zonula adherens formation. The marginal zone of the apical membrane may contain a crb- and sdt-dependent retention mechanism for adherens junction material that facilitates zonula adherens formation.
            crb and sdt encode critical components of a pathway that acts at the apical pole of epithelial cells to control their cytoarchitecture. Mosaic experiments suggest that sdt though not crb is required cell autonomously. Double mutant analysis suggests that sdt acts downstream of and is activated by crb.
            Zygotically active locus involved in the terminal developmental program in the embryo.
            A screen for X-linked genes that affect embryo morphology revealed sdt.
            sdt mutants display almost total absence of hypoderm, small patches of cuticle remain.
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 124 )
            Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            FlyMine - An integrated database for Drosophila genomics
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            Synonyms and Secondary IDs (24)
            Reported As
            Symbol Synonym
            BP1063
            CG12658
            CG15340
            CG15342
            CG1617
            anon-EST:fe2E6
            l(1)7Ef
            Secondary FlyBase IDs
            • FBgn0243505
            • FBgn0040927
            • FBgn0052717
            • FBgn0030024
            • FBgn0030023
            • FBgn0030022
            • FBgn0030021
            • FBgn0030020
            • FBgn0025216
            • FBgn0001465
            • FBgn0003349
            • FBgn0052716
            • FBgn0030019
            • FBgn0082843
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (234)