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Gene Dmel\osa

General Information
Symbol	Dmel\osa	Species	D. melanogaster
Name	osa	Annotation symbol	CG7467
Feature type	protein_coding_gene	FlyBase ID	FBgn0261885
Gene Model Status	Current	Stock availability	19 publicly available
Also Known As	eld
Genomic Location
Chromosome (arm)	3R	Recombination map	3-60.0
Cytogenetic map	90C1-90C2	Sequence location	3R:13,513,540..13,544,059 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene osa is referred to in FlyBase by the symbol Dmel\osa (CG7467, FBgn0261885). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA binding. There is experimental evidence for 12 unique biological process terms, many of which group under: biological regulation; post-embryonic organ morphogenesis; appendage development; system development; cellular process; regionalization; instar larval or pupal morphogenesis; segment specification; neuron differentiation; regulation of RNA metabolic process. 66 alleles are reported. The phenotypes of these alleles are annotated with: adult segment; organ system subdivision; thoracic segment; organ system; imaginal precursor; external compound sense organ; integumentary plate; embryonic/larval imaginal precursor; portion of tissue; epithelium. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: ARID/BRIGHT DNA-binding domain; Protein of unknown function DUF3518. Gene sequence location is 3R:13513540..13544059.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	osa: osa (J.A. Kennison) Isolated as a dominant suppressor of the antenna to leg transformation in Pc2 AntpNs double heterozygotes. Also acts as a dominant suppressor of AntpNs, Pc and Pcl alleles. Recessive embryonic lethality associated with all alleles. Also fails to complement In(3R)Antprv1 for viability.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation. Nakamura et al., 2008, Nucleic Acids Res. 36(12): 3905--3915 Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593
FB2012_01	References Kirilly et al., 2011, Neuron 72(1): 86--100 Sequence features BKN29156 HFA17022
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 90C1-90C2   (determined by in situ hybridisation); Limits computationally determined from genome sequence between P{PZ}l(3)0788207882 and P{EP}cpoEP3679
Experimentally Determined Cytological Location
Cytogenetic map Notes References 90B1-90D1   Location from complementation analysis with deficiency and duplication chromosomes (details unspecified).   (Kennison and Tamkun, 1988) 90C1-90C1   (determined by in situ hybridisation)   (Molnar et al., 2006) 90C1-90C2     (Treisman, 1998.3.9) 90C5-90C8   (determined by in situ hybridisation)   (Spradling et al., 1999) 90C1-90C2   (determined by in situ hybridisation)   (Treisman et al., 1997) 90B-90C   (determined by in situ hybridisation)   (Kennison, 1995) 90C5-90C8   90C7--8   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	3-60.0 (Kennison and Tamkun, 1988)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\osa for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) osa-RA FBtr0089582  NM_169775   10662   2703 osa-RB FBtr0089581  NM_079668   10701   2716 osa-RC FBtr0089583  NM_206506   10221   2556 osa-RD FBtr0301487  NM_001170168   10410   2716
Additional Transcript Data & Comments
Reported size (kB)	10.601 (sequence analysis) (Treisman et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein osa-PA   FBpp0088544   282.9   2703   7.49     NP_732263   AAF55457 osa-PB   FBpp0088543   284.1   2716   7.45     NP_524392   AAN13750 osa-PC   FBpp0088962   269.0   2556   7.46     NP_996228   AAS65166 osa-PD   FBpp0290702   284.1   2716   7.45     NP_001163639   ACZ94935
Additional Polypeptide Data & Comments
Reported size (kDa)	2715 (aa) (Treisman et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ARID/BRIGHT DNA-binding domain (IPR001606) Protein of unknown function DUF3518 (IPR021906)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201670   AA246646   AA540740   AA817002   AA820639   AA951850   AA979163   AE014297 AAF55457   AE014297 AAN13750   AE014297 AAS65166   AE014297 ACZ94935   AF053091 AAC06254   AI061890   AI259510   AI512196   AI516023   AI518047   AI531420   AI534303   AI534781   AI542914   AQ073805   AW942570   AX094190   AX260164   BG636592   BG637832   BG638410   BH214629   BH214630   BI353650   BI363323   BI364905   BI565522   BI566556   BI568230   BI583730   BI584877   BI585543   BI585962   BI586513   BI593017   BI606653   BI613223   BI613565   BI615475   BI618181   BI619442   BI620547   BI625072   BI627296   BI631421   BI632555   BT025960   CK662371   CL528179   CO154997   CO179346   CO186829   CO189375   CO262584   CO266176   CO266503   CO272222   CO277984   CO278776   CO284097   CO287399   CO299156   CO302609   CO307050   CO307115   CO308123   CO314976   CO317281   CO319108   CO343422   CZ468177   CZ474377   CZ477141   CZ484352   CZ484353   EC066665   EC074546   EC080354   EC081989   EC083248   EC084472   EC088931   EC195337   EC195925   EC196535   EC204146   EC204523   EC210302   EC214299   EC221394   EC223291   EC226344   EC229031   EC230931   EC236547   EC240532   EC244084   EC253731   EC262881   EL884960   EL884961   EL885206   EL885207   EV584535   EV585152   EV592129   EY198978   G00550
UniProtKB/Swiss-Prot	Q8IN94
UniProtKB/TrEMBL	Q7KSE8 E1JIN6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early   organism | ubiquitous   (Treisman et al., 1997) larval stage   wing disc   (Treisman et al., 1997) embryonic/larval salivary gland   • chromosome (Collins et al., 1999) morphogenetic furrow | anterior to   (Treisman et al., 1997) eye disc   (Treisman et al., 1997)
Additional Descriptive Data
	osa protein is ubiquitously distributed in early embryos and in wing discs. In eye discs, osa protein is present all over the disc but the strongest expression occurs in a band just anterior to the morphogenetic furrow. (Collins et al., 1999, Treisman et al., 1997)
Marker for
Subcellular Localization
CV Term	nucleus (Treisman et al., 1997)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal osaCC00445 lethal, with Scer\GAL41407 osaGD1502 lethal, with Scer\GAL4Act5C.PU osaGD1502 lethal, with Scer\GAL4bi-omb-Gal4 osaScer\UAS.cCa lethal, with Scer\GAL4Bx-MS1096 osaGD1502 lethal, with Scer\GAL4GMR.PF, Scer\GAL4ey.PH osaGD1502 lethal, with Scer\GAL4pnr-MD237 osaGD1502 lethal (with osa1) osaArv1 lethal (with osa2) osaArv1 lethal (with osaArv1) osa1 osa2 lethal | embryonic stage | germline clone | non-rescuable maternal effect | recessive osa3R-51-26 osa3R-68-15 osa3R-68-33 osa3R-108-31 osa3R-190-29 lethal | embryonic stage | recessive osa1 osa2 osa4F osa4H osa308 osa616 osa1946 osa2052 osa2438 osa2791 lethal | embryonic stage | recessive | rescuable maternal effect osa1 osa2 lethal | pupal stage | somatic clone osa308 lethal | recessive osa1 osa2 osa3 osa3R-51-26 osa3R-68-15 osa3R-68-33 osa3R-108-31 osa3R-190-29 osa4 osa00090 osa04662 osa06726 osaE17 osaj9C3 osaKG03117 Other Phenotypes Allele enhancer of variegation | dominant, with P{hsp26-pt-T}39C-5 osa1 osa2 enhancer of variegation | dominant, with P{hsp26-pt-T}39C-27 osa1 osa2 enhancer of variegation | dominant, with P{hsp26-pt-T}39C-31 osa1 osa2 increased cell death | somatic clone osa308 neuroanatomy defective | larval stage, with Scer\GAL41407 osaGD1502 non-enhancer of variegation, with P{wA}4-4 osa1 osa2 non-suppressor of variegation, with P{wA}4-4 osa1 osa2 size defective, with Scer\GAL4en-e16E osaScer\UAS.cCa visible, with Scer\GAL4bi-omb-Gal4 osaScer\UAS.cCa visible, with Scer\GAL4dpp.3KK osaC819 visible, with Scer\GAL4en-e16E osaScer\UAS.cCa visible, with Scer\GAL4ey.PH osaScer\UAS.cCa visible, with Scer\GAL4ey.PU osaScer\UAS.cCa visible, with Scer\GAL4MD-638 osaC819 visible, with Scer\GAL4nub-AC-62 osaScer\UAS.cCa visible, with Scer\GAL4pnr-MD237 osaScer\UAS.cCa visible, with Scer\GAL4salm-459.2 osaC819 visible, with Scer\GAL4vg.PM osaScer\UAS.cCa visible | cell autonomous | somatic clone osa308 visible | dominant osa1 osa308 osa616 osa1946 osa2052 osa2438 osa2791 osaKG03117 visible | heat sensitive, with Scer\GAL4salm-459.2 osaGD1502 visible | male, with Scer\GAL4MD-638 osaScer\UAS.cCa Phenotype manifest in Allele denticle | ectopic | germline clone | maternal effect osa3R-51-26 osa3R-190-29 osa3R-68-15 osa3R-108-31 osa3R-68-33 denticle belt | germline clone osa308 dorsocentral bristle, with Scer\GAL4pnr-MD237 osaAD.Scer\UAS.T:Hsim\VP16 osaScer\UAS.cCa dorsocentral bristle | supernumerary osaE17 osa616 osa14060 dorsocentral bristle | supernumerary (with osa4H) osa14060 dorsocentral bristle | supernumerary (with osa14060) osa4H embryonic/first instar larval cuticle | germline clone osa2 osa308 osa1 embryonic/first instar larval cuticle | germline clone | maternal effect osa3R-51-26 osa3R-190-29 osa3R-68-15 osa3R-108-31 osa3R-68-33 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 osaGD1502 embryonic epidermis | germline clone osa2 osa1 embryonic head | germline clone | maternal effect osa3R-51-26 osa3R-190-29 osa3R-68-15 osa3R-108-31 osa3R-68-33 embryonic segment | germline clone osa2 osa1 embryonic segment | germline clone | maternal effect osa3R-51-26 osa3R-190-29 osa3R-68-15 osa3R-108-31 osa3R-68-33 external sensory organ precursor cell | supernumerary (with osa4H) osa14060 external sensory organ precursor cell | supernumerary (with osa14060) osa4H eye, with Scer\GAL4ey.PH osaScer\UAS.cCa eye, with Scer\GAL4GMR.PF osaScer\UAS.cCa eye | somatic clone osa2791 osa1946 osa2438 osa308 osa616 osa2052 eye-antennal disc | somatic clone osa308 eye disc, with Scer\GAL4ey.PU osaScer\UAS.cCa eye disc | somatic clone osa308 ganglion mother cell | larval stage, with Scer\GAL41407 osaGD1502 leg | somatic clone | supernumerary osa308 leg | somatic clone | ventral compartment osa308 leg sensillum | somatic clone | supernumerary osa308 macrochaeta, with Scer\GAL4pnr-MD237 osaAD.Scer\UAS.T:Hsim\VP16 macrochaeta | dorsal, with Scer\GAL4pnr-MD237 osaScer\UAS.cCa Malpighian tubule tip cell osadust55 osadust31 osa00090 mesothoracic tergum | somatic clone osa308 osa616 microchaeta, with Scer\GAL4pnr-MD237 osaAD.Scer\UAS.T:Hsim\VP16 microchaeta | dorsal, with Scer\GAL4pnr-MD237 osaScer\UAS.cCa photoreceptor cell | somatic clone osa308 pupa, with Scer\GAL4bi-omb-Gal4 osaScer\UAS.cCa retina | somatic clone osa2791 osa1946 osa2438 osa308 osa616 osa2052 scutellar bristle, with Scer\GAL4pnr-MD237 osaAD.Scer\UAS.T:Hsim\VP16 osaScer\UAS.cCa sensillum campaniformium of dorsal radius, with Scer\GAL4bi-omb-Gal4 osaAD.Scer\UAS.T:Hsim\VP16 tarsal segment | somatic clone osa308 thorax, with Scer\GAL4pnr-MD237 osaScer\UAS.cCa wing osa2791 osa1946 osa2438 osa308 osa616 osa1 osaKG03117 osa2052 wing, with Scer\GAL4bi-omb-Gal4 osaScer\UAS.cCa wing, with Scer\GAL4en-e16E osaScer\UAS.cCa wing, with Scer\GAL4vg.PM osaScer\UAS.cCa wing & macrochaeta osa616 wing | anterior compartment | dorsal | somatic clone osa308 osa616 wing | ectopic osa308 osa616 wing blade | cell autonomous | somatic clone osa308 wing cell, with Scer\GAL4salm-459.2 osaC819 wing disc osa616 wing disc | somatic clone osa308 wing hair | cell autonomous | ectopic | somatic clone osa308 wing hair | ectopic, with Scer\GAL4MD-638 osaScer\UAS.cCa wing hair | ectopic, with Scer\GAL4nub-AC-62 osaScer\UAS.cCa wing hair | ectopic | heat sensitive, with Scer\GAL4salm-459.2 osaGD1502 wing hinge, with Scer\GAL4vg.PM osaScer\UAS.cCa wing margin | cell autonomous | somatic clone osa308 wing margin | heat sensitive, with Scer\GAL4salm-459.2 osaGD1502 wing margin bristle | ectopic osa616 wing margin bristle | ectopic | somatic clone osa308 wing sensillum | cell autonomous | ectopic | somatic clone osa308 wing sensillum | ectopic, with Scer\GAL4MD-638 osaScer\UAS.cCa wing sensillum | ectopic, with Scer\GAL4nub-AC-62 osaScer\UAS.cCa wing sensillum | ectopic | heat sensitive, with Scer\GAL4salm-459.2 osaGD1502 wing vein, with Scer\GAL4dpp.3KK osaC819 wing vein, with Scer\GAL4MD-638 osaC819 wing vein | cell autonomous | somatic clone osa308 wing vein | ectopic, with Scer\GAL4C-765 osaScer\UAS.cCa wing vein | ectopic, with Scer\GAL4en-e16E osaScer\UAS.cCa wing vein | ectopic, with Scer\GAL4MD-638 osaScer\UAS.cCa wing vein | ectopic, with Scer\GAL4nub-AC-62 osaScer\UAS.cCa wing vein | ectopic, with Scer\GAL4salm-459.2 osaC819 wing vein | heat sensitive, with Scer\GAL4salm-459.2 osaGD1502
Classical Alleles ( 61 )
For All Classical Alleles Show
Allele of osa Class Mutagen Stocks Known lesion osa2 hypomorphic allele - genetic evidence ethyl methanesulfonate 2 -- osaArv1 X ray 2 -- osa00090 P-element activity 1 Yes osa308 ethyl methanesulfonate 1 -- osac02745 piggyBac transposase 1 -- osaCB-0493-3 1 -- osad05189 P-element activity 1 -- osad10190 P-element activity 1 -- osaEY09619 P-element activity 1 -- osaf01858 piggyBac transposase 1 -- osaKG03117 P-element activity 1 -- osaMI02292 1 -- osa04539 P-element activity 0 Yes osa04662 P-element activity 0 Yes osa06726 P-element activity 0 Yes osa11 ethyl methanesulfonate 0 -- osa12 ethyl methanesulfonate 0 -- osa14060 0 Yes osa1946 0 -- osa1 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- osa2052 0 -- osa2438 0 -- osa2791 0 -- osa2823 ethyl methanesulfonate 0 -- osa3276 ethyl methanesulfonate 0 -- osa3 ethyl methanesulfonate 0 -- osa3R-108-31 ethyl methanesulfonate 0 -- osa3R-190-29 ethyl methanesulfonate 0 -- osa3R-51-26 ethyl methanesulfonate 0 -- osa3R-68-15 ethyl methanesulfonate 0 -- osa3R-68-33 ethyl methanesulfonate 0 -- osa4 ethyl methanesulfonate 0 -- osa4F ethyl methanesulfonate 0 -- osa4H ethyl methanesulfonate 0 Yes osa5 PM hybrid dysgenesis 0 -- osa616 ethyl methanesulfonate 0 Yes osa6 PM hybrid dysgenesis 0 Yes osa9-24 Delta2-3 0 Yes osaC819 0 -- osaCC00445 P-element activity 0 Yes osaCPTI003089 piggyBac transposase 0 Yes osadust31 ethyl methanesulfonate 0 -- osadust55 ethyl methanesulfonate 0 -- osaE17 0 -- osaE65 ethyl methanesulfonate 0 -- osaEP3-1074 0 Yes osaEP3-1386 0 Yes osaEP3-1591 0 -- osaEP3-473 0 Yes osaEP3-619 0 Yes osaEP3-900 0 -- osaEP3-941 0 Yes osaEP3190 P-element activity 0 -- osaj9C3 P-element activity 0 -- osaksh-11 ethyl methanesulfonate 0 -- osaksh-12 ethyl methanesulfonate 0 -- osaksh-13 X ray 0 -- osaL6530b P-element activity 0 -- osapjc-3 ethyl methanesulfonate 0 -- osas3263b P-element activity 0 -- osaunspecified 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of osa Class Mutagen Stocks Known lesion osaScer\UAS.cCa in vitro construct - regulatory fusion 2 Yes osaGD1502 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes osaGL00372 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes osaJF01207 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes osaAD.Scer\UAS.T:Hsim\VP16 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(3R)P14 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Disrupted in	Df(3R)DG2 (Staehling-Hampton et al., 1999) Df(3R)Exel6177 (Ryder, 2004.4.26) Df(3R)RD31 (Vazquez et al., 1999) Df(3R)RK8-21 (Kaminker et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1502} NA P{TRiP.GL00372} NA P{TRiP.JF01207} NA P{UAS-osa} NA P{UAS-osaAD} NA characterization construct P{UAS-osaRD} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}osaMI02292 NA P{}osa6 NA P{PTT-GC}osaCC00445 NA P{RS3}osaCB-0493-3 NA P{SUPor-P}osaKG03117 NA PBac{754.P.FSVS-0}osaCPTI003089 NA PBac{PB}osac02745 NA PBac{WH}osaf01858 NA insertion of mobile activating element P{EP}osaEP3-473 NA P{EP}osaEP3-619 NA P{EP}osaEP3-900 NA P{EP}osaEP3-941 NA P{EP}osaEP3-1074 NA P{EP}osaEP3-1386 NA P{EP}osaEP3-1591 NA P{EP}osaEP3190 NA P{EPgy2}osaEY09619 NA P{GSV1}osaC819 NA P{XP}osad05189 NA P{XP}osad10190 NA insertion of enhancer trap P{lacW}l(3)SH031SH031 No P{lacW}osaj9C3 No P{lacW}osaL6530b No P{lacW}osas3263b No P{luc-sniffer}osa9-24 No P{PZ}osa00090 No P{PZ}osa04539 No P{PZ}osa04662 No P{PZ}osa06726 No
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from direct assay (assigned by UniProtKB) (Collins et al., 1999)
Biological Process
CV term Evidence References
imaginal disc-derived leg morphogenesis inferred from mutant phenotype (Terriente-Félix and de Celis, 2009) imaginal disc-derived wing margin morphogenesis inferred from mutant phenotype (Terriente-Félix and de Celis, 2009) inferred from mutant phenotype (assigned by UniProtKB) (Treisman et al., 1997) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Dworkin and Gibson, 2006) imaginal disc-derived wing vein morphogenesis inferred from mutant phenotype (Terriente-Félix and de Celis, 2009) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) photoreceptor cell differentiation inferred from mutant phenotype (assigned by UniProtKB) (Treisman et al., 1997) positive regulation of transcription, DNA-dependent inferred from direct assay (Kal et al., 2000) regulation of epidermal growth factor receptor signaling pathway inferred from mutant phenotype (Terriente-Félix and de Celis, 2009) regulation of transcription, DNA-dependent inferred from direct assay (assigned by UniProtKB) (Collins et al., 1999) inferred from mutant phenotype (assigned by UniProtKB) (Vazquez et al., 1999) segment specification inferred from mutant phenotype (assigned by UniProtKB) (Treisman et al., 1997) wing disc dorsal/ventral pattern formation inferred from genetic interaction with Bx, ap (Bejarano et al., 2008) Wnt receptor signaling pathway inferred from mutant phenotype (assigned by UniProtKB) (Treisman et al., 1997)
Cellular Component
CV term Evidence References
brahma complex inferred from direct assay (Kal et al., 2000) nucleus inferred from direct assay (assigned by UniProtKB) (Treisman et al., 1997)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from sequence or structural similarity (Collins et al., 1998) non-traceable author statement (assigned by UniProtKB) (Treisman et al., 1997) contributes_to transcription coactivator activity inferred by curator from GO:0035060 (Kal et al., 2000)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
brahma complex traceable author statement (Fry and Peterson, 2001) nucleus non-traceable author statement (Collins et al., 1998)
Sequence Ontology: Class of Gene
	gene (Molnar et al., 2006) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions osa allele Gene References osa1 Antp (Vazquez et al., 1999) brm (Vazquez et al., 1999, Gutierrez et al., 2003) Pc (Kennison and Tamkun, 1988) tara (Calgaro et al., 2002, Gutierrez et al., 2003) tna (Gutierrez et al., 2003) osa2 Antp (Vazquez et al., 1999) brm (Vazquez et al., 1999) corto (Lopez et al., 2001) Dp (Staehling-Hampton et al., 1999) E(z) (Bajusz et al., 2001) net (Marenda et al., 2004) Pc (Kennison and Tamkun, 1988) sev (Maixner et al., 1998) osa3 Pc (Kennison and Tamkun, 1988) osa3R-51-26 gl(3R)53-6 (Luschnig et al., 2004) osa3R-68-15 gl(3R)53-6 (Luschnig et al., 2004) osa3R-108-31 gl(3R)53-6 (Luschnig et al., 2004) osa4 Pc (Kennison and Tamkun, 1988) osa4F lz (Kaminker et al., 2001) osa4H Chi (Heitzler et al., 2003) lz (Kaminker et al., 2001) osa11 brm (Vazquez et al., 1999) osa12 brm (Vazquez et al., 1999) osa00090 CycE (Brumby et al., 2002) Kr (Carrera et al., 1998, Abrell et al., 2000) peb (Wilk et al., 2004) osa308 brm (Collins et al., 1999) Bx (Milan et al., 2004) dpp (Treisman et al., 1997) gl(3R)53-6 (Luschnig et al., 2004) gro (Collins and Treisman, 2000) mor (Collins et al., 1999) ro (Treisman et al., 1997) sgg (Collins and Treisman, 2000) osa616 Chi (Heitzler et al., 2003) pnr (Heitzler et al., 2003) osa1946 ro (Chanut et al., 2000) osa2052 ro (Chanut et al., 2000) osa2438 ro (Chanut et al., 2000) osa2791 ro (Chanut et al., 2000) osa04539 Kr (Carrera et al., 1998) sev (Maixner et al., 1998) osaArv1 peb (Wilk et al., 2004) osaE17 Chi (Heitzler et al., 2003) osaE65 E2f (Staehling-Hampton et al., 1999) osaEP3-1074 Bx (Milan et al., 2004) osaEP3-1386 Bx (Milan et al., 2004) osaEP3-1591 Bx (Milan et al., 2004) osaksh-11 CycE (Brumby et al., 2002) Dp (Staehling-Hampton et al., 1999) osaksh-12 Dp (Staehling-Hampton et al., 1999) osaksh-13 Dp (Staehling-Hampton et al., 1999) osapjc-3 Dp (Staehling-Hampton et al., 1999) osas3263b CycE (Brumby et al., 2002) osaScer\UAS.cCa brm (Collins et al., 1999) mor (Collins et al., 1999) sgg (Collins and Treisman, 2000) Snr1 (Collins et al., 1999) osaunspecified CycE (Brumby et al., 2004) gl(3R)53-6 (Luschnig et al., 2004) unspecified Antp (Kennison and Tamkun, 1988, Lindsley and Zimm, 1992) Bx (Milan et al., 2001, Milan et al., 2004) Chi (Milan et al., 2001) ct (Krupp et al., 2005) HmgD (Ragab et al., 2006) HmgZ (Ragab et al., 2006) N (Treisman et al., 1997) Pc (Lindsley and Zimm, 1992) Pcl (Lindsley and Zimm, 1992) ph-p (Fauvarque et al., 1995) ro (Chanut et al., 2000) Scr (Gindhart and Kaufman, 1995)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261885 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42130
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23141 Dere\GG16758 Dgri\GH15872 Dmoj\GI23637 Dper\GL12242 Dpse\GA26236 Dsec\GM15347 Dsim\osa Dvir\GJ24712 Dwil\GK22507 Dyak\osa
Curated drosophilid orthologs	Dpse\GA20376   Dsim\osa (Begun, 2000.4.5) Dyak\osa (Begun, 2000.4.7)
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16051 NV31651 NV17809 LH15859 ISCW014189 FBgn0041640 FBgn0224484 FBgn0211788 FBgn0191696 FBgn0041660 FBgn0170267 JGI_V11_327651 FBgn0247610 FBgn0149849 FBgn0146363 FBgn0123343 FBgn0108988 FBgn0100135 CPIJ010152 BGIBMGA010273 ACYPI006497 GB17648 AGAP001786 AAEL017280 PHUM080380 GLEAN_13586
Stocks & Reagents
Stocks Listed in FlyBase ( 19 )
Bloomington	5948 w*; P{UAS-osa}s2/CyO 3616 osa2/TM6B, Tb1 31266 y1 v1; P{TRiP.JF01207}attP2
Harvard	- P{XP}osad05189 - P{XP}osad10190 - PBac{PB}osac02745 - PBac{WH}osaf01858
Kyoto	108497 w*; P{UAS-osa}s2/CyO 107130 osa2/TM6B, Tb1 123202 w1118; P{RS3}osaCB-0493-3 106514 In(3R)Antprv1, osaArv1/TM3, Sb1
VDRC	v7810 w1118; P{GD1502}v7810
	More stocks available...
Genomic Clones ( 2 )
	BACR14A01 BACR21K03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 92 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP16063
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH03026 GM24566 LD04852 LD21959 LD32583 LD33446 LD37452 LD41757 LD43956 LP02936 LP22529 RE48103 RE50103 RH10878 RH22410 RH24177 RH25149 RH25669 RH26471 RH36234 RH39637 RH42333 RH42743 RH45133 RH48766 RH50476 RH51715 RH60989 RH63308 RH64902 RH68565 RH73263 SD02449 SD07089 SD07791 SD09430 SD14337 SD26364
Other clones	8007 15652 16610 33922 35218 59717 62757 98125 119028 146859 148225 163543 164292 175065 214479.55 524324 CK01.9D.B10 CK01.9D.D10 CK01.27B.E7 CK01.54B.C5 CK01.75C.F6 CK01.101D.B6 CK01.102A.C12 Dmel_dig_009796_0633_0495 Dmel_dig_010088_0587_3637 Dmel_neb_006394_0691_2288 DMG2-chr3R.14.060.a.1 EK023720 EK044111 EK059155 EK065747 EK085839 EK106317 EK127955 EK131545 EK145224 EK146624 EK165230 EK174640 EK184541 EK238636 EK253359 EK253636 EK256701 EK267615 EK276252 EN05154 EP16745 IP16163 IP16363 LD05567 LD20534 LD24466
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261885 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42130
Antibody Information
	monoclonal (Treisman et al., 1997)
Other Information
Discoverer
	Kennison, 1983.
Etymology
	"osa" means "fate" in Norwegian. (Kennison, 1995)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: osa CG7467 (Gong, 1999.11)
Source for database merge of	Source for merge of: osa anon-WO0118547.314 anon-WO0172774.126 (FlyBase, 1996-) Source for merge of: osa C819 (Terriente-Félix and de Celis, 2009) Source for merge of: osa eld (Maixner et al., 1998, Vazquez et al., 1999) Source for merge of: osa l(3)j9C3 (Spradling et al., 1999)
Additional comments	Source for merge of osa anon-WO0118547.314 anon-WO0172774.126 was sequence comparison (date:051113). (FlyBase, 1996-) Fails to complement In(3R)Antprv1 for viability.
Other Comments
	osa acts as a positive modulator of ap activity in the wing. osa mediates activation of some ap target genes and repression of others. (Milan et al., 2004) D.melanogaster contains two distinct brahma (BRM) complexes: the BAP complex, defined by the presence of osa protein and the absence of both polybromo and Bap170 proteins, and the PBAP complex, which contains the polybromo and Bap170 proteins but lacks the osa protein. (Mohrmann et al., 2004) osa negatively regulates neural expression of ac/sc. (Heitzler et al., 2003) Area matching Drosophila EST AA201670. (Davies, 2001.3.15) brm interacts with osa to regulate the expression of the Antp P2 promoter. (Vazquez et al., 1999) osa may be involved in signalling through N or wg pathways. osa protein is expressed ubiquitously in the early embryos and in imaginal discs and is localised to the nucleus where results suggests it counteracts the effects of wg on its target genes. (Treisman et al., 1997) Mosaic analysis in eye discs suggests a function for osa in cell proliferation and/or survival and neuronal differentiation. (Treisman et al., 1997) Mutations in osa enhance the phentype of mutations in brm. (Vazquez et al., 1995) Mutations cannot rescue the lethality of the Df(1)ph-2-Psc1 interaction, demonstrating that trx group mutations cannot suppress Pc group phenotypes. (Cheng et al., 1994) Dosage-dependent modifier of AntpNs. (Talbert and Garber, 1994) Identification: dominant suppressor of the antenna to leg transformation in Pc2 AntpNs double heterozygotes. (Lindsley and Zimm, 1992) Dominant suppressor of AntpNs, Pc and Pcl alleles. Recessive embryonic lethality is associated with all alleles. (Lindsley and Zimm, 1992) osa is one of the 18 loci identified in a screen for dominant modifiers of Pc and/or Antp phenotypes. Alleles of Pc, Pcl, Scm, Dll, brm, kto, Scr and trx show clear dominant enhancement or suppression of AntpScx, whereas alleles of vtd, Vha55, Su(Pc)37D, urd, mor, skd and osa do not. (Kennison and Tamkun, 1988)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079668 NM_169775 NM_206506 NM_001170168 RefSeq (Proteins) • NP_524392 NP_732263 NP_996228 NP_001163639 DDBJ / EMBL / GenBank • AA201670 AA540740 AAC06254 AAF55457 AAN13750 AAS65166 ACZ94935 AF053091 AI061890 AQ073805 AW942570 AX094190 AX260164 BH214629 BH214630 BI565522 CL528179 CZ468177 CZ474377 CZ477141 CZ484352 CZ484353 G00550 Entrez Gene - A searchable database of RefSeq genes. • 42130 UniProtKB/Swiss-Prot • Q8IN94 UniProtKB/TrEMBL • E1JIN6 Q7KSE8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • ARID/BRIGHT DNA-binding domain (IPR001606) Protein of unknown function DUF3518 (IPR021906)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261885 DRSC - Results from RNAi screens. • FBgn0261885 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261885 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42130 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42130 modMine - Data generated by the modENCODE project. • FBgn0261885 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16051 NV31651 NV17809 LH15859 ISCW014189 FBgn0041640 FBgn0224484 FBgn0211788 FBgn0191696 FBgn0041660 FBgn0170267 JGI_V11_327651 FBgn0247610 FBgn0149849 FBgn0146363 FBgn0123343 FBgn0108988 FBgn0100135 CPIJ010152 BGIBMGA010273 ACYPI006497 GB17648 AGAP001786 AAEL017280 PHUM080380 GLEAN_13586
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	anon-WO0118547.314   anon-WO0172774.126   C819 (Molnar et al., 2006) CG7467 (Khokhar et al., 2008, Klebes et al., 2005, Stempfl et al., 2002, Molnar et al., 2006, Ni et al., 2008, Ni et al., 2011.7.19) E(E2F)3C (Staehling-Hampton et al., 1999) eld (Luschnig et al., 2004, Luschnig et al., 2004, Begun, 2001, Abrell et al., 2000, Begun and Whitley, 2000, Treisman, 1998.3.9, Spradling et al., 1999, Thomas and Wassarman, 1999, Collins et al., 1998, Treisman et al., 1997, Fauvarque et al., 1995, ) en(lz)4F/4H (Kaminker et al., 2001) l(3)00090 (Carrera et al., 1998, ) l(3)04539 (Maixner et al., 1998) l(3)j9C3   osa (Gan et al., 2010, Arancio et al., 2010, Curtis et al., 2011, Khokhar et al., 2008, Dworkin and Gibson., 2006, Mathew et al., 2005, Dworkin and Gibson, 2006, Buszczak et al., 2007, Marenda et al., 2003, Hirose et al., 2001, Molnar et al., 2006, Dietzl et al., 2007, Chalkley et al., 2008, Cukier et al., 2008, Kankel et al., 2007, Vázquez et al., 2008, Petruk et al., 2008, Remaud et al., 2008, Melicharek et al., 2008, Ayroles et al., 2009, Carrera et al., 2008, Terriente-Félix and de Celis, 2009, Schaaf et al., 2009, Salzer et al., 2010, Herr et al., 2010, Di Stefano et al., 2011, Beltran et al., 2007, Neumüller et al., 2011, Nakamura et al., 2008) OSA (Eberharter and Becker, 2004, Schwartz and Pirrotta, 2007, Ataman, 2006, Chang et al., 2007, Taylor-Harding et al., 2004, Moshkin et al., 2007, Moshkin et al., 2009) Osa (Carrera et al., 2004, Klochendler-Yeivin et al., 2002, Schuettengruber, 2006, Secombe et al., 2007, Martin et al., 2007, Nie et al., 2009, Glavic et al., 2009, Diop et al., 2008, Baig et al., 2010) p300 (Kal et al., 2000)
Name Synonym	eyelid (Tulin et al., 2002, Bonini and Fortini, 1999, Abrell et al., 2000, Treisman et al., 1997, ) eye lid (Shandala et al., 1998) osa (Bejarano et al., 2008, Cukier et al., 2008, Remaud et al., 2008, Salzer et al., 2010)
Secondary FlyBase IDs
	FBgn0003013 FBgn0010708 FBgn0011421 FBgn0015384 FBgn0046467 FBgn0062101 FBgn0259443
References ( 176 )
Generate a list of	All references relating to this gene ( 176 )
List References by type	Research paper  ( 106 ) Supplementary material  ( 8 ) Review  ( 19 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 16 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 4 ) Curated genome annotation  ( 2 ) Patent  ( 2 ) Book  ( 1 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 ) Conference report  ( 1 )
Recent research papers ( 11 )
	Brooks et al., 2011, Genome Res. 21(2): 193--202 Conservation of an RNA regulatory map between Drosophila and mammals. [FBrf0213079] Curtis et al., 2011, Dev. Biol. 350(2): 534--547 Histone lysine demethylases function as co-repressors of SWI/SNF remodeling activities during Drosophila wing development. [FBrf0212876] Di Stefano et al., 2011, Genes Dev. 25(1): 17--28 Functional antagonism between histone H3K4 demethylases in vivo. [FBrf0212709] Gabilondo et al., 2011, Mech. Dev. 128(3-4): 208--221 A targeted genetic screen identifies crucial players in the specification of the Drosophila abdominal Capaergic neurons. [FBrf0213290] Kirilly et al., 2011, Neuron 72(1): 86--100 Intrinsic epigenetic factors cooperate with the steroid hormone ecdysone to govern dendrite pruning in Drosophila. [FBrf0216319] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Arancio et al., 2010, Genetics 185(1): 129--140 The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors. [FBrf0213976] Baig et al., 2010, Genetics 184(3): 731--744 The chromatin-remodeling protein Osa interacts with CyclinE in Drosophila eye imaginal discs. [FBrf0210399] Gan et al., 2010, Cell Res. 20(7): 763--783 Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq. [FBrf0211191] Herr et al., 2010, Dev. Biol. 344(1): 36--51 Geminin and Brahma act antagonistically to regulate EGFR-Ras-MAPK signaling in Drosophila. [FBrf0211319] Salzer et al., 2010, Genetics 184(1): 185--197 The retinal determination gene eyes absent is regulated by the EGF receptor pathway throughout development in Drosophila. [FBrf0209690] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...