FlyBase Gene Report: Dmel\osa

Predictions / Assertions

Summaries

Gene Group (FlyBase)

BRAHMA ASSOCIATED PROTEINS COMPLEX -: Brahma (BRM) complexes are ATP-dependent chromatin remodeling complexes that regulate nucleosome organization. Two BRM complexes have been identified in D.mel, BAP and PBAP. (Adapted from FBrf0192510).
A-T RICH INTERACTION DOMAIN TRANSCRIPTION REGULATORS -: The A-T Rich Interaction Domain (ARID) transcription regulators are non-sequence specific DNA binding proteins. The ARID domain comprises a variant helix-turn-helix motif, accompanied by additional DNA-contacting loop structures. Many have roles in chromatin remodelling and regulation of general transcription. (Adapted from PMID:15922553).

Protein Function (UniProtKB)

Trithorax group (trxG) protein required for embryonic segmentation, development of the notum and wing margin, and photoreceptor differentiation. Required for the activation of genes such as Antp, Ubx and Eve. Binds to DNA without specific affinity, suggesting that it is recruited to promoters by promoter-specific proteins. Essential component of the Brahma complex, a multiprotein complex which is the equivalent of the yeast SWI/SNF complex and acts by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. This complex can both serve as a transcriptional coactivator or corepressor, depending on the context. Acts as an essential coactivator for Zeste, which recruits the whole complex to specific genes. In contrast, it acts as a corepressor for Wg target genes, possibly via an interaction with Pan and Gro. It also acts as a negative regulator for proneural achaete-scute, when it is directly recruited by Pan and Chi. Also represses E2f activation.

(UniProt, Q8IN94)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

osa: osa (J.A. Kennison)

Isolated as a dominant suppressor of the antenna to leg transformation in Pc2 AntpNs double heterozygotes. Also acts as a dominant suppressor of AntpNs, Pc and Pcl alleles. Recessive embryonic lethality associated with all alleles. Also fails to complement In(3R)Antprv1 for viability.

Summary (Interactive Fly)

transcription factor - Bright family - associates with the Brahma chromatin remodeling complex - antagonizes Wingless signaling during Drosophila development and affects patterning of the eye imaginal disc

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\osa or the JBrowse view of Dmel\osa for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

gene

(Molnar et al., 2006)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

osa-RA

FBtr0089582

NM_169775

10675

2703

osa-RB

FBtr0089581

NM_079668

10714

2716

osa-RC

FBtr0089583

NM_206506

10234

2556

osa-RD

FBtr0301487

NM_001170168

10423

2716

osa-RE

FBtr0335024

NM_001275751

9940

2555

osa-RF

FBtr0335025

NM_001275752

10243

2559

Additional Transcript Data and Comments

Reported size (kB)

10.601 (sequence analysis)

(Treisman et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

osa-PA

FBpp0088544

282.9

2703

7.49

NP_732263

AAF55457

osa-PB

FBpp0088543

284.1

2716

7.45

NP_524392

AAN13750

osa-PC

FBpp0088962

269.0

2556

7.46

NP_996228

AAS65166

osa-PD

FBpp0290702

284.1

2716

7.45

NP_001163639

ACZ94935

osa-PE

FBpp0307030

268.8

2555

7.46

NP_001262680

AGB96060

osa-PF

FBpp0307031

269.3

2559

7.50

NP_001262681

AGB96061

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2716 aa isoforms: osa-PB, osa-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

2715 (aa)

(Treisman et al., 1997)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Brahma complex, which is composed of Brm, Osa, Mor, Snr1/Bap45, Bap111/Dalao, Bap55, Bap60 and Bap47. Interacts with Pnr and Chi via its EHD domain.

(UniProt, Q8IN94)

Domain

The ARID domains mediates the binding to DNA.

(UniProt, Q8IN94)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\osa using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

osa_1

Linkouts

Gene Ontology (18 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

inferred from direct assay

(assigned by UniProt )

(Collins et al., 1999)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

imaginal disc-derived leg morphogenesis

non-traceable author statement

(assigned by UniProt )

(Treisman et al., 1997)

Biological Process (14 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

inferred from mutant phenotype

(Terriente-Félix and de Celis, 2009)

imaginal disc-derived wing margin morphogenesis

inferred from mutant phenotype

(Terriente-Félix and de Celis, 2009)

inferred from mutant phenotype

(assigned by UniProt )

(Treisman et al., 1997)

imaginal disc-derived wing morphogenesis

inferred from mutant phenotype

(Dworkin and Gibson, 2006)

imaginal disc-derived wing vein morphogenesis

inferred from mutant phenotype

(Terriente-Félix and de Celis, 2009)

negative regulation of neuroblast proliferation

inferred from genetic interaction with FLYBASE:numb; FB:FBgn0002973

(Janssens et al., 2014)

inferred from genetic interaction with FLYBASE:brat; FB:FBgn0010300

(Janssens et al., 2014)

neuroblast fate commitment

inferred from mutant phenotype

(Eroglu et al., 2014)

photoreceptor cell differentiation

inferred from mutant phenotype

(assigned by UniProt )

(Treisman et al., 1997)

positive regulation of transcription, DNA-templated

inferred from physical interaction with FLYBASE:ham; FB:FBgn0045852

(Eroglu et al., 2014)

inferred from direct assay

(Kal et al., 2000)

regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Bonnay et al., 2014)

regulation of transcription, DNA-templated

inferred from direct assay

(assigned by UniProt )

(Collins et al., 1999)

inferred from mutant phenotype

(assigned by UniProt )

(Vazquez et al., 1999)

segment specification

inferred from mutant phenotype

(assigned by UniProt )

(Treisman et al., 1997)

wing disc dorsal/ventral pattern formation

inferred from genetic interaction with FLYBASE:Bx; FB:FBgn0265598, FLYBASE:ap; FB:FBgn0267978

(Bejarano et al., 2008)

Wnt signaling pathway

inferred from mutant phenotype

(assigned by UniProt )

(Treisman et al., 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

chromatin remodeling

inferred from electronic annotation with InterPro:IPR021906

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

brahma complex

inferred from direct assay

(Mohrmann et al., 2004, Kal et al., 2000)

nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

nucleus

inferred from direct assay

(assigned by UniProt )

(Treisman et al., 1997)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

organism | ubiquitous

(Treisman et al., 1997)

larval stage

wing disc

(Treisman et al., 1997)

embryonic/larval salivary gland

• chromosome

(Collins et al., 1999)

morphogenetic furrow | anterior to

(Treisman et al., 1997)

eye disc

(Treisman et al., 1997)

Additional Descriptive Data

Protein was localized along the entire length of all chromosomes. In vitro binding assays showed that osa protein bound DNA without sequence specificity.

(Collins et al., 1999, Treisman et al., 1997)

Marker for

Subcellular Localization

CV Term

Evidence

References

brahma complex

inferred from direct assay

(Mohrmann et al., 2004, Kal et al., 2000)

nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

nucleus

inferred from direct assay

(assigned by UniProt )

(Treisman et al., 1997)

Expression Deduced from Reporters

Reporter: PBac{754.P.FSVS-0}osa^CPTI003089

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\osa in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\osa

View exonic expression by tissue for Dmel\osa

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0261885

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7467

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 65 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of osa

Transgenic constructs containing regulatory region of osa

Deletions and Duplications ( 5 )

Not disrupted in

Df(3R)P14

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Disrupted in

Df(3R)DG2

(Staehling-Hampton et al., 1999)

Df(3R)Exel6177

(Ryder, 2004.4.26)

Df(3R)RD31

(Vazquez et al., 1999)

Df(3R)RK8-21

(Kaminker et al., 2001)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal, with Scer\GAL4^Act5C.PU

osa^GD1502

lethal, with Scer\GAL4^bi-omb-Gal4

osa^UAS.cCa

lethal, with Scer\GAL4^Bx-MS1096

osa^GD1502

lethal, with Scer\GAL4^c564

osa^GD1502

lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

osa^GD1502

lethal, with Scer\GAL4^pnr-MD237

osa^GD1502

lethal (with Df(3R)BSC790)

osa^{MI02292-TG4.0}

lethal (with osa¹)

osa^Arv1

lethal (with osa²)

osa^Arv1

lethal (with osa^Arv1)

lethal | embryonic stage | germline clone | non-rescuable maternal effect | recessive

lethal | embryonic stage | recessive

lethal | embryonic stage | recessive | rescuable maternal effect

lethal | recessive

lethal - all die before end of embryonic stage | recessive

lethal - all die before end of pupal stage | somatic clone

osa³⁰⁸

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

osa^GD1502

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

osa^GD1502

viable

osa^{MI02292-GFSTF.0}

viable, with Scer\GAL4^tin.CΔ4

osa^GD1502

Other Phenotypes

Allele

enhancer of variegation | dominant, with P{hsp26-pt-T}39C-5

enhancer of variegation | dominant, with P{hsp26-pt-T}39C-27

enhancer of variegation | dominant, with P{hsp26-pt-T}39C-31

increased cell death | somatic clone

osa³⁰⁸

increased cell death | somatic clone | third instar larval stage, with Scer\GAL4^Act.PU

osa^HMS01738

neuroanatomy defective | larval stage

osa^GD1502

neuroanatomy defective | larval stage, with Scer\GAL4^GMR9D11

osa^{dsRNA.UAS.cEa}

neuroanatomy defective | larval stage, with Scer\GAL4^wor.PA

osa^{dsRNA.UAS.cEa}

neuroanatomy defective | larval stage | somatic clone

osa³⁰⁸

non-enhancer of variegation, with In(1)w^m4

osa²

non-enhancer of variegation, with P{wA}4-4

non-suppressor of variegation, with In(1)w^m4

osa²

non-suppressor of variegation, with P{wA}4-4

pain response defective | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

osa^GD1502

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

osa^GD1502

size defective, with Scer\GAL4^en-e16E

osa^UAS.cCa

some die during embryonic stage | recessive

some die during pupal stage | somatic clone

osa³⁰⁸

visible, with Scer\GAL4^bi-omb-Gal4

visible, with Scer\GAL4^dpp.3KK

osa^C819

visible, with Scer\GAL4^en-e16E

osa^UAS.cCa

visible, with Scer\GAL4^ey.PH

osa^UAS.cCa

visible, with Scer\GAL4^ey.PU

osa^UAS.cCa

visible, with Scer\GAL4^MD-638

osa^C819

visible, with Scer\GAL4^nub-AC-62

osa^UAS.cCa

visible, with Scer\GAL4^pnr-MD237

visible, with Scer\GAL4^salm-459.2

osa^C819

visible, with Scer\GAL4^vg.PM

osa^UAS.cCa

visible | adult stage, with Scer\GAL4^en.PU

osa^JF01207

visible | cell autonomous | somatic clone

osa³⁰⁸

visible | dominant

visible | heat sensitive, with Scer\GAL4^salm-459.2

osa^GD1502

visible | male, with Scer\GAL4^MD-638

osa^UAS.cCa

Phenotype manifest in

Allele

denticle | ectopic | germline clone | maternal effect

denticle belt | germline clone

osa³⁰⁸

dorsocentral bristle, with Scer\GAL4^pnr-MD237

dorsocentral bristle | supernumerary

dorsocentral bristle | supernumerary (with osa^4H)

osa¹⁴⁰⁶⁰

dorsocentral bristle | supernumerary (with osa¹⁴⁰⁶⁰)

osa^4H

embryonic/first instar larval cuticle | germline clone

embryonic/first instar larval cuticle | germline clone | maternal effect

embryonic epidermis | germline clone

embryonic head | germline clone | maternal effect

embryonic segment | germline clone

embryonic segment | germline clone | maternal effect

external sensory organ precursor cell | supernumerary (with osa^4H)

osa¹⁴⁰⁶⁰

external sensory organ precursor cell | supernumerary (with osa¹⁴⁰⁶⁰)

osa^4H

eye, with Scer\GAL4^ey.PH

osa^UAS.cCa

eye, with Scer\GAL4^GMR.PF

osa^UAS.cCa

eye | somatic clone

eye-antennal disc | somatic clone

osa³⁰⁸

eye disc, with Scer\GAL4^ey.PU

osa^UAS.cCa

eye disc | somatic clone

osa³⁰⁸

ganglion mother cell | larval stage

osa^GD1502

larval neuroblast | larval stage

osa^GD1502

leg | somatic clone | supernumerary

osa³⁰⁸

leg sensillum | somatic clone | supernumerary

osa³⁰⁸

leg ventral compartment | somatic clone

osa³⁰⁸

macrochaeta, with Scer\GAL4^pnr-MD237

osa^VP16.AD.UAS

macrochaeta | dorsal, with Scer\GAL4^pnr-MD237

osa^UAS.cCa

macrochaeta | ectopic, with Scer\GAL4^pnr-MD237

osa^RD.UAS

Malpighian tubule tip cell

mesothoracic tergum | somatic clone

microchaeta, with Scer\GAL4^pnr-MD237

osa^VP16.AD.UAS

microchaeta | dorsal, with Scer\GAL4^pnr-MD237

osa^UAS.cCa

microchaeta | ectopic, with Scer\GAL4^pnr-MD237

osa^RD.UAS

photoreceptor cell | somatic clone

osa³⁰⁸

posterior crossvein, with Scer\GAL4^en.PU

osa^JF01207

pupa, with Scer\GAL4^bi-omb-Gal4

osa^UAS.cCa

retina | somatic clone

scutellar bristle, with Scer\GAL4^pnr-MD237

sensillum campaniformium of dorsal radius, with Scer\GAL4^bi-omb-Gal4

osa^VP16.AD.UAS

tarsal segment | somatic clone

osa³⁰⁸

thorax, with Scer\GAL4^pnr-MD237

osa^UAS.cCa

type II neuroblast | somatic clone | supernumerary

osa³⁰⁸

type II neuroblast | supernumerary, with Scer\GAL4^wor.PA

osa^{dsRNA.UAS.cEa}

wing

wing, with Scer\GAL4^bi-omb-Gal4

osa^UAS.cCa

wing, with Scer\GAL4^en.PU

osa^JF01207

wing, with Scer\GAL4^en-e16E

osa^UAS.cCa

wing, with Scer\GAL4^vg.PM

osa^UAS.cCa

wing & macrochaeta

osa⁶¹⁶

wing | ectopic

wing blade | cell autonomous | somatic clone

osa³⁰⁸

wing blade anterior compartment | dorsal | somatic clone

wing cell, with Scer\GAL4^salm-459.2

osa^C819

wing disc

osa⁶¹⁶

wing disc | somatic clone

osa³⁰⁸

wing hair | cell autonomous | ectopic | somatic clone

osa³⁰⁸

wing hair | ectopic, with Scer\GAL4^MD-638

osa^UAS.cCa

wing hair | ectopic, with Scer\GAL4^nub-AC-62

osa^UAS.cCa

wing hair | ectopic | heat sensitive, with Scer\GAL4^salm-459.2

osa^GD1502

wing hinge, with Scer\GAL4^vg.PM

osa^UAS.cCa

wing margin | cell autonomous | somatic clone

osa³⁰⁸

wing margin | heat sensitive, with Scer\GAL4^salm-459.2

osa^GD1502

wing margin bristle | ectopic

osa⁶¹⁶

wing margin bristle | ectopic, with Scer\GAL4^bi-omb-Gal4

osa^RD.UAS

wing margin bristle | ectopic | somatic clone

osa³⁰⁸

wing pouch | somatic clone | third instar larval stage, with Scer\GAL4^Act.PU

osa^HMS01738

wing sensillum | cell autonomous | ectopic | somatic clone

osa³⁰⁸

wing sensillum | ectopic, with Scer\GAL4^MD-638

osa^UAS.cCa

wing sensillum | ectopic, with Scer\GAL4^nub-AC-62

osa^UAS.cCa

wing sensillum | ectopic | heat sensitive, with Scer\GAL4^salm-459.2

osa^GD1502

wing vein, with Scer\GAL4^bi-omb-Gal4

osa^RD.UAS

wing vein, with Scer\GAL4^dpp.3KK

osa^C819

wing vein, with Scer\GAL4^MD-638

osa^C819

wing vein | cell autonomous | somatic clone

osa³⁰⁸

wing vein | ectopic, with Scer\GAL4^C-765

osa^UAS.cCa

wing vein | ectopic, with Scer\GAL4^en-e16E

osa^UAS.cCa

wing vein | ectopic, with Scer\GAL4^MD-638

osa^UAS.cCa

wing vein | ectopic, with Scer\GAL4^nub-AC-62

osa^UAS.cCa

wing vein | ectopic, with Scer\GAL4^salm-459.2

osa^C819

wing vein | heat sensitive, with Scer\GAL4^salm-459.2

osa^GD1502

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ARID1A

11 of 15

Yes

Hsap\ARID1B

11 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Arid1a

10 of 15

Yes

Mmus\Arid1b

9 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (4)

Rnor\Arid1a

5 of 13

Yes

Rnor\Arid1b

4 of 13

Yes

Rnor\Arid3b

1 of 13

Rnor\Arid3c

1 of 13

Xenopus tropicalis (Western clawed frog) (4)

Xtro\arid1a

5 of 12

Yes

Xtro\arid1b

4 of 12

Yes

Xtro\arid3a

2 of 12

Xtro\arid3b

1 of 12

Danio rerio (Zebrafish) (8)

Drer\arid1b

11 of 15

Yes

Drer\arid1ab

5 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\let-526

11 of 15

Yes

Cele\cfi-1

2 of 15

Cele\arid-1

1 of 15

Arabidopsis thaliana (thale-cress) (1)

Atha\LFR

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\ECM5

1 of 15

Yes

Scer\RSC9

1 of 15

Yes

Scer\SWI1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\rsc9

1 of 12

Yes

Spom\sol1

1 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900R9 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\osa

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA26236

Drosophila persimilis

Dper\GL12242

Drosophila willistoni

Dwil\GK26793

Drosophila willistoni

Dwil\GK22507

Drosophila virilis

Dvir\GJ24712

Drosophila mojavensis

Dmoj\GI23637

Drosophila grimshawi

Dgri\GH15872

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501ZK )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17026026

Glossina morsitans

Tsetse fly

Gmor\GMOY003630

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_12388

Mayetiola destructor

Hessian fly

Mdes\Mdes006443

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL017280

Anopheles darlingi

American malaria mosquito

Adar\ADAC008556

Anopheles gambiae

Malaria mosquito

Agam\AGAP001786

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ010152

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W016J )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA010273

Danaus plexippus

Monarch butterfly

Dple\DPOGS207345-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002206

Apis florea

Little honeybee

Aflo\2862666676

Apis mellifera

Western honey bee

Amel\GB44899

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP18498

Bombus terrestris

Buff-tailed bumblebee

Bter\15212568

Linepithema humile

Argentine ant

Lhum\LH15859

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0006cf

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG019750

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_09239

Tribolium castaneum

Red flour beetle

Tcas\TC013586

Pediculus humanus

Human body louse

Phum\PHUM080380

Rhodnius prolixus

Kissing bug

Rpro\RPRC005301

Cimex lectularius

Bed bug

Clec\CLEC012210

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006497

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_18729

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X014S )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR006121

Strigamia maritima

European centipede

Smar\SMAR001561

Ixodes scapularis

Black-legged tick

Isca\ISCW014189

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58954.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78505.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g14180

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g14160

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_327651

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00GP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-SwiL

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000014057

Gallus gallus

Domestic chicken

Ggal\ARID1B

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027551

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000000360

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

2 of 10

htk

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

ARID1A; AT-rich interaction domain 1A

model of Coffin-Siris syndrome 2

IEA

(FlyBase, 2019-)

ARID1B; AT-rich interaction domain 1B

model of Coffin-Siris syndrome 1

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

osa⁰⁰⁰⁹⁰

ameliorates intellectual disability

modeled by Hsap\MECP2^UAS.FL

(Vonhoff et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ARID1A; AT-rich interaction domain 1A

11 of 15

603024

COFFIN-SIRIS SYNDROME 2; CSS2

Coffin-Siris syndrome 2

ARID1B; AT-rich interaction domain 1B

11 of 15

614556

COFFIN-SIRIS SYNDROME 1; CSS1

Coffin-Siris syndrome 1

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

osa - Act5C

anti bait coimmunoprecipitation, molecular weight estimation by staining

(Collins et al., 1999)

osa - Bap111

anti bait coimmunoprecipitation, molecular weight estimation by staining, protein cross-linking with a bifunctional reagent, Identification by mass spectrometry

(Mashtalir et al., 2018, Collins et al., 1999)

osa - Bap55

anti bait coimmunoprecipitation, molecular weight estimation by staining

(Collins et al., 1999)

osa - Bap60

protein cross-linking with a bifunctional reagent, Identification by mass spectrometry, anti bait coimmunoprecipitation, molecular weight estimation by staining, experimental knowledge based, anti tag coimmunoprecipitation

(Mashtalir et al., 2018, Guruharsha et al., 2011, Collins et al., 1999)

osa - CG17002

(Guruharsha et al., 2011)

osa - Cdk8

nuclear magnetic resonance

(Guruharsha et al., 2011)

osa - Chi

(Fiedler et al., 2015)

osa - Cpsf6

anti bait coimmunoprecipitation, western blot

(Guruharsha et al., 2011)

osa - CycE

(Baig et al., 2010)

osa - Larp4B

(Guruharsha et al., 2011)

osa - MED14

(Guruharsha et al., 2011)

osa - MED17

(Guruharsha et al., 2011)

osa - MED18

(Guruharsha et al., 2011)

osa - MED23

(Guruharsha et al., 2011)

osa - Ptip

anti bait coimmunoprecipitation, molecular weight estimation by staining, western blot

(Guruharsha et al., 2011)

osa - Snr1

(Collins et al., 1999)

osa - Usp10

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot, ion exchange chromatography, molecular sieving, experimental knowledge based

(Guruharsha et al., 2011)

osa - brm

(Nakayama et al., 2012, Guruharsha et al., 2011, Chalkley et al., 2008, Mohrmann et al., 2004, Kal et al., 2000, Collins et al., 1999)

osa - d4

anti tag coimmunoprecipitation, protein cross-linking with a bifunctional reagent, Identification by mass spectrometry

(Mashtalir et al., 2018)

osa - dsh

(Guruharsha et al., 2011)

osa - ebi

ion exchange chromatography, molecular sieving, western blot, anti bait coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Guruharsha et al., 2011)

osa - mor

(Guruharsha et al., 2011, Chalkley et al., 2008, Mohrmann et al., 2004, Collins et al., 1999)

osa - polybromo

protein cross-linking with a bifunctional reagent, Identification by mass spectrometry

(Mashtalir et al., 2018)

osa - sd

anti tag coimmunoprecipitation, anti tag western blot

(Jin et al., 2013)

osa - smt3

anti tag coimmunoprecipitation, comigration in sds page, anti tag western blot

(Pirone et al., 2017)

osa - su(Hw)

anti bait coimmunoprecipitation, western blot

(Vorobyeva et al., 2013)

osa - tna

anti bait coimmunoprecipitation, western blot, two hybrid

(Monribot-Villanueva et al., 2013)

osa - yki

anti bait coimmunoprecipitation, peptide massfingerprinting

(Jin et al., 2013)

osa - z

far western blotting, autoradiography

(Kal et al., 2000)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

brm

(Gutierrez et al., 2003, Collins et al., 1999)

suppressible

brm

(Collins et al., 1999)

enhanceable

corto

(Lopez et al., 2001)

suppressible

geminin

(Herr et al., 2010)

suppressible

htt

(Dietz et al., 2015)

enhanceable

mor

(Collins et al., 1999)

enhanceable

smt3

(Monribot-Villanueva et al., 2013)

enhanceable

Snr1

(Collins et al., 1999)

enhanceable

tara

(Gutierrez et al., 2003)

enhanceable

tna

(Gutierrez et al., 2003)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Antp

suppressible

(Ragab et al., 2006, Vazquez et al., 1999)

Antp|Pc

suppressible

(Kennison and Tamkun, 1988)

asf1

suppressible

(Moshkin et al., 2002)

brat

enhanceable

(Janssens et al., 2014)

brm

enhanceable

(Gutierrez et al., 2003, Collins et al., 1999)

suppressible

brm|osa

(Terriente-Félix and de Celis, 2009)

enhanceable

(Terriente-Félix and de Celis, 2009)

suppressible

(Bejarano et al., 2008, Milan et al., 2004)

enhanceable

(Bejarano et al., 2008, Milan et al., 2004)

Bx|ap

suppressible

(Bejarano et al., 2008)

Chi

enhanceable

(Heitzler et al., 2003)

Chi

suppressible

(Heitzler et al., 2003)

corto

enhanceable

(Lopez et al., 2001)

enhanceable

(Krupp et al., 2005)

suppressible

(Krupp et al., 2005)

CycE

suppressible

(Brumby et al., 2004, Brumby et al., 2002)

Dp|E2f1

enhanceable

(Staehling-Hampton et al., 1999)

dpp

enhanceable

(Treisman et al., 1997)

Dref

suppressible

(Nakamura et al., 2008, Hirose et al., 2001)

E2f1|Dp

enhanceable

(Staehling-Hampton et al., 1999)

E(z)

suppressible

(Bajusz et al., 2001)

E(z)

enhanceable

(Bajusz et al., 2001)

gcm

enhanceable

(Popkova et al., 2012)

gcm

suppressible

(Popkova et al., 2012)

gcm

enhanceable

brm|osa

(Popkova et al., 2012)

enhanceable

(Abrell et al., 2000, Carrera et al., 1998)

enhanceable

(Kaminker et al., 2001)

Marcal1

enhanceable

(Morimoto et al., 2016)

Marcal1

suppressible

(Morimoto et al., 2016)

mor

suppressible

(Collins et al., 1999)

net

enhanceable

(Terriente-Félix and de Celis, 2009, Marenda et al., 2004)

net|px

suppressible

brm|osa

(Terriente-Félix and de Celis, 2009)

suppressible

(Kennison and Tamkun, 1988)

peb

enhanceable

(Wilk et al., 2004)

pnr

enhanceable

(Heitzler et al., 2003)

Ras85D

enhanceable

(Terriente-Félix and de Celis, 2009)

Ras85D

suppressible

(Terriente-Félix and de Celis, 2009)

Ras85D|sev

suppressible

(Maixner et al., 1998)

enhanceable

(Terriente-Félix and de Celis, 2009)

suppressible

(Chanut et al., 2000, Treisman et al., 1997)

enhanceable

(Jin et al., 2013)

sgg

suppressible

(Collins and Treisman, 2000)

sgg

enhanceable

(Collins and Treisman, 2000)

tara

enhanceable

(Gutierrez et al., 2003)

tna

enhanceable

(Monribot-Villanueva et al., 2013, Gutierrez et al., 2003)

tou

suppressible