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Gene Ncra\QF

General Information
Symbol	Ncra\QF	Species	N. crassa
Name	Quinic acid utilization activator	Annotation symbol
Feature type	engineered_foreign_gene	FlyBase ID	FBgn0261910
Gene Model Status	Not Applicable	Stock availability	25 publicly available
Also Known As	QF
Genomic Location
Chromosome (arm)		Recombination map
Cytogenetic map		Sequence location
Summary Information
Automatically generated summary See sections below for more information	The gene Quinic acid utilization activator is referred to in FlyBase by the symbol Ncra\QF (FBgn0261910). It is a engineered_foreign_gene from Neurospora crassa. Its molecular function is unknown. The biological processes in which it is involved are not known. 26 alleles are reported. The phenotype of these alleles is annotated with: nucleolus. It has no annotated transcripts. Gene has not been localized to the genome sequence.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Mapped Features
Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
modENCODE Anatomy RNA-Seq
modENCODE Development RNA-Seq
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with dmNcra\QUAS.cPa Ncra\QFPsc-ET40 Other Phenotypes Allele neuroanatomy defective | somatic clone, with Scer\GAL4Mz19, Ten-mGS9267, Ten-mNcra\QUAS.cHa Ncra\QFMz19.PH paralytic | heat sensitive, with shits1.Ncra\QUAS Ncra\QFET49 smell perception defective, with Ncra\QSScer\UAS.cPa, Scer\GAL4acj6-PG63, shits1.Ncra\QUAS Ncra\QFGH146.PP smell perception defective, with shits1.-FRT.Ncra\QUAS Ncra\QFGH146.PP smell perception defective, with shits1.Ncra\QUAS Ncra\QFGH146.PP Phenotype manifest in Allele nucleolus, with dmNcra\QUAS.cPa Ncra\QFunspecified
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of Ncra\QF Class Mutagen Stocks Known lesion Ncra\QFPsc-ET40 P-element activity 3 -- Ncra\QFET49 1 -- Ncra\QFExt2-ET31 P-element activity 1 -- Ncra\QFskd-ET6 P-element activity 1 -- Ncra\QFSu(z)2-ET9 P-element activity 1 -- Ncra\QF3.1.w- 0 -- Ncra\QF6.1.w- 0 -- Ncra\QFCG9426-ET10 P-element activity 0 -- Ncra\QFET12 P-element activity 0 -- Ncra\QFET14 P-element activity 0 -- Ncra\QFKdm2-ET11 P-element activity 0 -- Ncra\QFkermit-ET13 P-element activity 0 -- Ncra\QFlola-ET8 P-element activity 0 -- Ncra\QFMYPT-75D-MI00314-QF site specific recombinase 0 Yes Ncra\QFunspecified 0 --
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ncra\QF Class Mutagen Stocks Known lesion Ncra\QFGH146.PP in vitro construct - regulatory fusion 5 Yes Ncra\QFM2ET in vitro construct 5 Yes Ncra\QFID in vitro construct - regulatory fusion 4 Yes Ncra\QFET in vitro construct 2 Yes Ncra\QFnompC.PP in vitro construct - regulatory fusion 2 Yes Ncra\QFTrpA1.PP in vitro construct - regulatory fusion 2 Yes Ncra\QFiav.PP in vitro construct - regulatory fusion 1 Yes Ncra\QFMz19.PH in vitro construct - regulatory fusion 1 Yes Ncra\QFsynj.PP in vitro construct - regulatory fusion 1 Yes Ncra\QFTbh.PS in vitro construct - regulatory fusion 1 Yes Ncra\QFtrpl.PP in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	engineered_foreign_gene (FlyBase Curators, 1992-)
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ncra\QF allele Gene References
External Data
Linkouts
Orthologs
OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search No orthologs identified Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Ortholog(s) in Drosophila melanogaster (None identified)
No D. melanogaster orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 25 )
Bloomington	30014 w1118; PBac{GH146-QF.P}11 30015 w1118; PBac{GH146-QF.P}53 41573 w*; PBac{Mz19-QF.H}VK00037 30016 y1 w1118; P{M2ET-QF}ET6 33233 w*; S1/CyO; P{ID.QF}Q33A 30017 y1 w1118; P{ET-QF}ET9/CyO 30018 y1 w1118; P{M2ET-QF}ET31/CyO 30019 y1 w1118; P{M2ET-QF}ET40/CyO 30020 y1 w1118; P{ET-QF}ET49/TM6B, Tb1 30037 w1118; PBac{GH146-QF.P}53 P{QUAS-mtdTomato-3xHA}24A 30043 y1 w1118; P{QUAS-mtdTomato-3xHA}14 P{M2ET-QF}ET40/CyO 33230 y1 w*; P{ID.QF}CG7370Q2A CG42689Q2A
	More stocks available...
Genomic Clones ( 0 )
cDNA Clones ( 0 )
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Ncra\QF is a transcriptional activator that binds to a 16bp sequence found naturally in the Neurospora crassa qa gene cluster (copies of this 16bo sequence are referred to as Ncra\QUAS). Ncra\QF can thus be used to activate transcription of reporter constructs containing Ncra\QUAS regulatory sequences. (Potter et al., 2010)
Tag or Foreign Gene Data
	Foreign sequence; species == Neurospora crassa; gene == 'QF'; UniProtKB/Swiss-Prot:P11638.
External Crossreferences & Linkouts
Sequence Crossreferences
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Ncra\QF   qa-1F (Potter et al., 2010) QF (Potter et al., 2010, Petersen and Stowers, 2011, Stowers, 2011.9.28, Stowers, 2011)
Name Synonym	Quinic acid utilization activator
Secondary FlyBase IDs
References ( 20 )
Research paper	Hong et al., 2012, Nature 484(7393): 201--207 Teneurins instruct synaptic partner matching in an olfactory map. [FBrf0218059] Mosca et al., 2012, Nature 484(7393): 237--241 Trans-synaptic Teneurin signalling in neuromuscular synapse organization and target choice. [FBrf0218053] Prieto-Godino et al., 2012, PLoS Biol. 10(10): e1001400 Embryonic origin of olfactory circuitry in Drosophila: contact and activity-mediated interactions pattern connectivity in the antennal lobe. [FBrf0219665] Gohl et al., 2011, Nat. Methods 8(3): 231--237 A versatile in vivo system for directed dissection of gene expression patterns. [FBrf0213287] Li et al., 2011, PLoS ONE 6(11): e28269 The hector G-Protein Coupled Receptor Is Required in a Subset of fruitless Neurons for Male Courtship Behavior. [FBrf0216834] Petersen and Stowers, 2011, PLoS ONE 6(9): e24531 A Gateway MultiSite Recombination Cloning Toolkit. [FBrf0215584] Pitman et al., 2011, Curr. Biol. 21(10): 855--861 A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body. [FBrf0213725] Stowers, 2011, Fly 5(4): 371--378 An efficient method for recombineering GAL4 and QF drivers. [FBrf0216432] Venken et al., 2011, Nat. Methods 8(9): 737--743 MiMIC: a highly versatile transposon insertion resource for engineering Drosophila melanogaster genes. [FBrf0216331] Potter et al., 2010, Cell 141(3): 536--548 The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. [FBrf0210697]
Supplementary material	Hong et al., 2012, Nature 484(7393): Online methods. [FBrf0219840] Mosca et al., 2012, Nature 484(7393): Online methods. [FBrf0219881] Gohl et al., 2011, Nat. Methods 8(3): Online methods. [FBrf0213241] Gohl et al., 2011, Nat. Methods 8(3): Supplementary material. [FBrf0213393] Potter et al., 2010, Cell 141(3): Extended Experimental Procedures. [FBrf0210925] Potter et al., 2010, Cell 141(3): Table S1. Q system resources - transgenics and recombinants generated in this study. [FBrf0210936]
Personal communication to FlyBase	Stowers, 2011.9.28, PBac{trpl-QF.P}VK00027. PBac{trpl-QF.P}VK00027. [FBrf0216367] Potter and Luo, 2010.3.5, P{QUAS-dm.P} from Potter and Luo. P{QUAS-dm.P} from Potter and Luo. [FBrf0210937]
FlyBase analysis	FlyBase Curators, 1992-, Summary information describing the origin of tags and foreign genes in FlyBase. Summary information describing the origin of tags and foreign genes in FlyBase. [FBrf0199194] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]