FB2025_01 , released February 20, 2025
Gene: Dmel\mid
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General Information
Symbol
Dmel\mid
Species
D. melanogaster
Name
midline
Annotation Symbol
CG6634
Feature Type
protein_coding_gene
FlyBase ID
FBgn0261963
Gene Model Status
Current
Stock Availability
31 publicly available
Gene Summary
T-box transcription factors that participate in cardiac fate specificity - regulates wing development by repressing wingless and hedgehog (Interactive Fly, mid )
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

nmr2, extra, neuromancer2, los, lost in space

Key Links
Genomic Location
Cytogenetic map
25E2-25E2
Sequence location
Recombination map
2-17
RefSeq locus
NT_033779 REGION:5461639..5467610
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (32 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables DNA-binding transcription repressor activity, RNA polymerase II-specific
inferred from direct assay
(Formaz-Preston et al., 2012)
inferred from mutant phenotype
(Formaz-Preston et al., 2012)
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding
inferred from direct assay
(Formaz-Preston et al., 2012)
enables transcription corepressor binding
inferred from physical interaction with FLYBASE:gro; FB:FBgn0001139
(Formaz-Preston et al., 2012)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables DNA-binding transcription factor activity, RNA polymerase II-specific
inferred from biological aspect of ancestor with PANTHER:PTN004251338
(GO Reference Genome Project, 2011-)
enables DNA-binding transcription factor activity
(InterPro Project Members, 2004-)
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding
inferred from electronic annotation with InterPro:IPR001699
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN004251338
(GO Reference Genome Project, 2011-)
Biological Process (24 terms)
Terms Based on Experimental Evidence (21 terms)
CV Term
Evidence
References
involved_in axon guidance
inferred from mutant phenotype
(Liu et al., 2009)
involved_in axonogenesis
inferred from mutant phenotype
(Kolodziej et al., 1995)
involved_in cardioblast cell fate commitment
inferred from mutant phenotype
(Qian et al., 2005)
involved_in central nervous system development
inferred from mutant phenotype
(Leal et al., 2009)
inferred from genetic interaction with FLYBASE:HGTX; FB:FBgn0040318
(Leal et al., 2009)
involved_in chorion-containing eggshell pattern formation
inferred from mutant phenotype
(Fregoso Lomas et al., 2013)
involved_in embryonic heart tube development
inferred from mutant phenotype
(Qian et al., 2005)
involved_in fasciculation of motor neuron axon
inferred from mutant phenotype
(Kolodziej et al., 1995)
involved_in ganglion mother cell fate determination
inferred from mutant phenotype
(Gaziova and Bhat, 2009)
involved_in gonad development
inferred from mutant phenotype
(Tripathy et al., 2014)
involved_in heart development
inferred from mutant phenotype
(Miskolczi-McCallum et al., 2005)
involved_in heart process
inferred from direct assay
(Qian et al., 2008)
involved_in leg disc pattern formation
inferred from genetic interaction with FLYBASE:H15; FB:FBgn0016660
(Svendsen et al., 2009)
involved_in negative regulation of gene expression
inferred from mutant phenotype
(Leal et al., 2009)
inferred from genetic interaction with FLYBASE:HGTX; FB:FBgn0040318
(Leal et al., 2009)
involved_in negative regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Formaz-Preston et al., 2012)
involved_in neuroblast development
inferred from mutant phenotype
(Buescher et al., 2006)
involved_in neuroblast fate specification
inferred from mutant phenotype
(Buescher et al., 2006)
involved_in neuron fate specification
inferred from mutant phenotype
(Gaziova and Bhat, 2009)
involved_in positive regulation of transcription by RNA polymerase II
inferred from direct assay
(Gaziova and Bhat, 2009)
involved_in regulation of cardioblast differentiation
inferred from mutant phenotype
(Reim et al., 2005)
involved_in regulation of cell fate specification
inferred from mutant phenotype
(Kumar et al., 2015)
inferred from genetic interaction with FLYBASE:H15; FB:FBgn0016660
(Svendsen et al., 2009)
involved_in regulation of nervous system development
inferred from mutant phenotype
(Gaziova and Bhat, 2009)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in cell fate specification
inferred from biological aspect of ancestor with PANTHER:PTN000137774
(GO Reference Genome Project, 2011-)
involved_in heart development
inferred from biological aspect of ancestor with PANTHER:PTN002601330
(GO Reference Genome Project, 2011-)
involved_in positive regulation of DNA-templated transcription
inferred from electronic annotation with InterPro:IPR046360
(InterPro Project Members, 2004-)
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN004251338
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in chromatin
inferred from biological aspect of ancestor with PANTHER:PTN004251338
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN004251338
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR018186, InterPro:IPR036960
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
T-BOX TRANSCRIPTION FACTORS -
The T-box transcription factors are sequence-specific DNA binding proteins that regulate transcription. They are characterized by a conserved DNA-binding immunoglobulin-fold domain of 180-200 amino acids, known as T-box, named after its founding member, the mouse T protein. (Adapted from FBrf0218841).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
mid: midline
Homozygous embryonic lethal; denticle bands defective in ventral midline.
Summary (Interactive Fly)

T-box transcription factors that participate in cardiac fate specificity - regulates wing development by repressing wingless and hedgehog

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\mid for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VMR2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.52

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mid-RA
FBtr0079093
NM_135083
2920
580
mid-RB
FBtr0310358
NM_001273167
2139
580
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
mid-PA
FBpp0078726
62.9
580
8.31
Q9VMR2
NP_608927
AAF52250
mid-PB
FBpp0302039
62.9
580
8.31
Q9VMR2
NP_001260096
AGB92632
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

580 aa isoforms: mid-PA, mid-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mid using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.66

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
ventral midline of embryo
(Wheeler et al., 2009)
embryonic stage 4 -- 6
antennal anlage in statu nascendi
(Fisher et al., 2012)
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
hypopharynx anlage in statu nascendi
(Fisher et al., 2012)
organism | segmentally repeated
(Fisher et al., 2012)
ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 5
organism | segment polarity expression pattern
(Prpic et al., 2003)
embryonic stage 7 -- 8
antennal anlage
(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
hypopharynx anlage
(Fisher et al., 2012)
organism | segmentally repeated
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
dorsal ectoderm
(Fisher et al., 2012)
hypopharynx inclusive primordium
(Fisher et al., 2012)
organism | segmentally repeated
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
ventral head epidermis primordium
(Fisher et al., 2012)
embryonic stage 11
cardiogenic mesoderm
(Qian et al., 2005)
embryonic stage 11 -- 12
larval foregut atrium | precursor

Comment: reported as atrium primordium

(Fisher et al., 2012)
cardiogenic mesoderm
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
hypopharynx proper primordium
(Fisher et al., 2012)
organism | segmentally repeated
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
ventral head epidermis primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 12
embryonic ventral nerve cord
(Prpic et al., 2003)
embryonic stage 13 -- 16
larval foregut atrium
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
embryonic hypopharynx
(Fisher et al., 2012)
embryonic/larval dorsal vessel
(Fisher et al., 2012)
labral segment
(Fisher et al., 2012)
organism | segmentally repeated
(Fisher et al., 2012)
ventral head epidermis
(Fisher et al., 2012)
embryonic stage 14 -- 15
heart primordium
(Prpic et al., 2003)
embryonic stage 15
embryonic brain
(Prpic et al., 2003)
third instar larval stage
leg disc | proximal | ventral
(Prpic et al., 2003)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult heart
(Qian et al., 2008)
single cell RNA-seq
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
direct flight muscle precursor cell
(Zappia et al., 2020)
Additional Descriptive Data

mid transcript is expressed in the developing dorsal vessel from embryonic stage 11.

(Qian et al., 2005)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 10
neuroblast NB7-1
(Gaziova and Bhat, 2009)
neuroblast NB7-2
(Gaziova and Bhat, 2009)
neuroblast NB3-2
(Gaziova and Bhat, 2009)
embryonic stage 10 -- 11
neuroblast NB7-4
(Gaziova and Bhat, 2009)
neuroblast NB2-5
(Gaziova and Bhat, 2009)
embryonic stage 10 -- 16
ventral midline of embryo
(Gaziova and Bhat, 2009)
embryonic stage 11
neuroblast NB1-2
(Gaziova and Bhat, 2009)
neuroblast NB6-2
(Gaziova and Bhat, 2009)
neuroblast NB6-4
(Gaziova and Bhat, 2009)
embryonic stage 13 -- 16
larval RP2 motor neuron
(Gaziova and Bhat, 2009)
third instar larval stage
peripodial epithelium of wing disc
(Fu et al., 2016)
wing disc
(Fu et al., 2016)
wandering third instar larval stage -- prepupal stage P2
photoreceptor neuron
(Das et al., 2013)
oogenesis stage S8 -- S10B & adult stage | female
main body follicle cell | posterior | subset
• nucleus
(Fregoso Lomas et al., 2013)
oogenesis stage S8 -- S10
follicle cell | posterior
(Fregoso Lomas et al., 2016)
Additional Descriptive Data

In wild-type third instar larvae, mid is ubiquitously expressed in the nucleus in both peripodial epithelium and the disc proper.

(Fu et al., 2016)

mid staining is found only to the posterior of the dorsal appendage forming follicle cells, where br expression is present but much weaker than in the roof cells. It is also not expressed in the br-negative cells on the dorsal midline, between the two patches of roof cells.

(Fregoso Lomas et al., 2013)

mid protein is expressed in a large number of neurons in the ventral midline. It was localized to specific midline neuroblasts at embryonic stages 10 and 11. At stage 13, it was found to be expressed in a row 5 GMC, dubbed an "M-neuron", that appears to change into an extra RP2. It's relation to the NB4-2->GMC-1->RP2/sib lineage is described.

(Gaziova and Bhat, 2009)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mid in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mid
Transgenic constructs containing regulatory region of mid
Aberrations (Deficiencies and Duplications) ( 27 )
Inferred from experimentation ( 27 )
Gene disrupted in
Df(2L)BSC810
(Kumar et al., 2015)
Df(2L)cl1
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Tearle and Nusslein-Volhard, 1987, Kotarski et al., 1983)
Df(2L)cl2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl7
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Tearle and Nusslein-Volhard, 1987, Kotarski et al., 1983)
Df(2L)cl-h1
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl-h2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)cl-h3
(Harbecke and Lengyel, 1995, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983)
Df(2L)Exel6012
(Kumar et al., 2015, Ryder, 2004.4.26)
Df(2L)Gpdh1-A
(Kumar et al., 2015, Miskolczi-McCallum et al., 2005, Buescher et al., 2004, Harbecke and Lengyel, 1995, Tearle and Nusslein-Volhard, 1987)
Df(2L)odd-4.25
(Nusslein-Volhard et al., 1984)
Df(2L)sc19-4
(Leal et al., 2009, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-12
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)x528
(Kumar et al., 2015, Leal et al., 2009, Miskolczi-McCallum et al., 2005, Buescher et al., 2004)
Gene not disrupted in
Df(2L)BSC169
(Kumar et al., 2015)
Df(2L)cl-h4
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)Exel6011
(Kumar et al., 2015)
Df(2L)H15-x4
(Miskolczi-McCallum et al., 2005)
Df(2L)sc19-1
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-5
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-6
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-7
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)sc19-8
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)tkv2
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Df(2L)tkv3
(Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Gene duplicated in
Dp(2;1)B19
Gene partially disrupted in
Df(2L)ΔG04
(Stevens et al., 2022)
Df(2L)ΔG04-F11
(Stevens et al., 2022)
Inferred from location ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4ptc.PU
lethal (with Df(2L)Exel6012)
lethal (with mid1)
lethal (with mid2)
lethal (with midB23)
lethal - all die before end of first instar larval stage
lethal - all die during embryonic stage
lethal - all die during embryonic stage | recessive
partially lethal - majority die, with Scer\GAL469B
partially lethal - majority die, with Scer\GAL4da.G32
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Other Phenotypes
Allele
abnormal cell number | embryonic stage
abnormal cell polarity, with Scer\GAL4tin.CΔ4
abnormal neuroanatomy
abnormal neuroanatomy (with Df(2L)x528)
abnormal neuroanatomy | embryonic stage
abnormal neuroanatomy | embryonic stage, with Scer\GAL4ac.13
abnormal neuroanatomy | embryonic stage (with Df(2L)Exel6012)
abnormal neuroanatomy | embryonic stage (with mid1)
abnormal neuroanatomy | embryonic stage (with mid2)
abnormal neuroanatomy | embryonic stage (with midlos1)
decreased body size | larval stage, with Scer\GAL4ptc-559.1
decreased cell number | embryonic stage, with Scer\GAL4ac.13
short lived, with Scer\GAL4twi.PG
some die during embryonic stage
some die during embryonic stage, with Scer\GAL469B
some die during embryonic stage, with Scer\GAL4da.G32
some die during embryonic stage | recessive
visible | adult stage, with Scer\GAL4ptc.PU
visible | male limited, with Scer\GAL4bi-omb-Gal4
Phenotype manifest in
Allele
abdominal neuroblast NB1-1
abdominal neuroblast NB1-1 (with Df(2L)x528)
abdominal neuroblast NB2-4
abdominal neuroblast NB2-4 (with Df(2L)x528)
adult heart | progressive, with Scer\GAL4how-24B, Scer\GAL80ts.αTub84B
adult heart | progressive, with Scer\GAL4tin.CΔ4, Scer\GAL80ts.αTub84B
adult heart | progressive, with Scer\GAL4twi.PG
chordotonal organ & axon | lateral
denticle | ectopic, with Scer\GAL4ptc-559.1
denticle | ectopic, with Scer\GAL4sca-P309
denticle row 1 & abdominal 1 ventral denticle belt
denticle row 1 & abdominal 2 ventral denticle belt
denticle row 1 & abdominal 3 ventral denticle belt
denticle row 1 & abdominal 4 ventral denticle belt
denticle row 1 & abdominal 5 ventral denticle belt
denticle row 1 & abdominal 6 ventral denticle belt
denticle row 1 & abdominal 7 ventral denticle belt
denticle row 1 & abdominal 8 ventral denticle belt
denticle row 2 & abdominal 1 ventral denticle belt
denticle row 2 & abdominal 2 ventral denticle belt
denticle row 2 & abdominal 3 ventral denticle belt
denticle row 2 & abdominal 4 ventral denticle belt
denticle row 2 & abdominal 5 ventral denticle belt
denticle row 2 & abdominal 6 ventral denticle belt
denticle row 2 & abdominal 7 ventral denticle belt
denticle row 2 & abdominal 8 ventral denticle belt
denticle row 3, with Scer\GAL4da.G32
denticle row 3 & abdominal 1 ventral denticle belt
denticle row 3 & abdominal 2 ventral denticle belt
denticle row 3 & abdominal 3 ventral denticle belt
denticle row 3 & abdominal 4 ventral denticle belt
denticle row 3 & abdominal 5 ventral denticle belt
denticle row 3 & abdominal 6 ventral denticle belt
denticle row 3 & abdominal 7 ventral denticle belt
denticle row 3 & abdominal 8 ventral denticle belt
denticle row 4, with Scer\GAL4da.G32
denticle row 6, with Scer\GAL4da.G32
dorsal appendage
dorsal appendage, with Scer\GAL4T155
dorsal appendage | somatic clone
dorsal vessel primordium, with Scer\GAL4how-24B, Scer\GAL4twi.PG
dorsal vessel wall cell
dorsal vessel wall cell, with Scer\GAL4how-24B, Scer\GAL4twi.PG
dorsal vessel wall cell, with Scer\GAL4tin.CΔ4
dorsal vessel wall cell (with Df(2L)cl-h2)
dorsal vessel wall cell | ectopic, with Scer\GAL4how-24B
egg chorion
egg chorion | somatic clone
embryonic/first instar larval cuticle
embryonic/larval cuticle, with Scer\GAL4ptc-559.1
embryonic/larval cuticle, with Scer\GAL4sca-P309
embryonic/larval dorsal vessel, with Scer\GAL4pnr-MD237
embryonic/larval dorsal vessel, with Scer\GAL4twi.PB
embryonic/larval heart
embryonic/larval heart, with Scer\GAL4how-24B, Scer\GAL4twi.PG
embryonic/larval heart, with Scer\GAL4pnr-MD237
embryonic/larval heart, with Scer\GAL4twi.PB
embryonic/larval hypodermal muscle cell
embryonic/larval hypodermal muscle cell (with mid1)
embryonic/larval hypodermal muscle cell (with mid8A69N1)
embryonic cardioblast
embryonic dMP2 neuron | decreased number, with Scer\GAL4ac.13
follicle cell | somatic clone
ganglion mother cell GMC1-1a
germline cell | embryonic stage
germline cell | embryonic stage (with Df(2L)Exel6012)
gonad | embryonic stage
gonad | embryonic stage (with mid1)
gonad | embryonic stage (with mid2)
gonad | embryonic stage (with midB23)
gonadal sheath proper primordium
heart primordium
larval head, with Scer\GAL4ptc-559.1
larval longitudinal connective
larval longitudinal connective | embryonic stage
larval ostial pore
larval ostial pore (with Df(2L)cl-h2)
larval pericardial cell
larval posterior commissure | embryonic stage
larval RP2 motor neuron | ectopic
larval RP2 motor neuron | ectopic (with Df(2L)Exel6012)
larval RP2 motor neuron | ectopic (with mid1)
larval RP2 motor neuron | ectopic (with mid2)
larval RP2 motor neuron | ectopic (with midlos1)
larval VL3/4 motor neuron
larval VO2 motor neuron
lateral cord neuron
leg, with Scer\GAL4ptc.PU
mesothoracic tarsal bristle, with Scer\GAL4Act5C.PI
mesothoracic tarsal bristle longitudinal row 1 | ectopic | somatic clone, with Scer\GAL4Act5C.PI
mesothoracic tarsal bristle longitudinal row 8 | ectopic | somatic clone, with Scer\GAL4Act5C.PI
mesothoracic tibial apical bristle | ectopic, with Scer\GAL4bi-omb-Gal4
mesothoracic tibial apical bristle | ectopic | somatic clone, with Scer\GAL4Act5C.PI
mesothoracic tibial spur bristle | ectopic, with Scer\GAL4bi-omb-Gal4
mesothoracic tibial spur bristle | ectopic | somatic clone, with Scer\GAL4Act5C.PI
metathoracic tarsal bristle, with Scer\GAL4Act5C.PI
motor neuron
muscle cell of lateral transverse muscle 3, with Scer\GAL4twi.PG
muscle cell of lateral transverse muscle 3 (with mid1)
muscle cell of lateral transverse muscle 3 (with mid8A69N1)
muscle cell of lateral transverse muscle 4
muscle cell of lateral transverse muscle 4, with Scer\GAL4how-24B
muscle cell of lateral transverse muscle 4, with Scer\GAL4Mef2.PR
muscle cell of lateral transverse muscle 4 (with mid1)
muscle cell of lateral transverse muscle 4 (with mid8A69N1)
neuroblast NB2-5
neuroblast NB2-5 (with Df(2L)x528)
neuron | increased number
presumptive embryonic/larval central nervous system
prothoracic tarsal bristle, with Scer\GAL4Act5C.PI
prothoracic tibial transverse row bristle | ectopic, with Scer\GAL4bi-omb-Gal4
segment border muscle cell, with Scer\GAL4twi.PG
segment border muscle cell (with mid1)
segment border muscle cell (with mid8A69N1)
sensory neuron | embryonic stage
sex comb | ectopic, with Scer\GAL4bi-omb-Gal4
symmetrical commissure
ventral denticle belt
ventral denticle belt, with Scer\GAL469B
ventral denticle belt, with Scer\GAL4da.G32
ventral denticle belt, with Scer\GAL4prd.RG1
wing, with Scer\GAL4ptc.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (17)
Hsap\TBX20
11 of 14
Yes
Yes
Hsap\MGA
4 of 14
No
Yes
Hsap\TBX19
4 of 14
No
No
Hsap\TBXT
4 of 14
No
No
Hsap\EOMES
3 of 14
No
Yes
Hsap\TBR1
3 of 14
No
Yes
Hsap\TBX1
3 of 14
No
No
Hsap\TBX2
3 of 14
No
No
4  
Hsap\TBX3
3 of 14
No
No
5  
Hsap\TBX4
3 of 14
No
No
Hsap\TBX5
3 of 14
No
No
1  
Hsap\TBX6
3 of 14
No
No
Hsap\TBX10
3 of 14
No
No
Hsap\TBX15
3 of 14
No
Yes
Hsap\TBX18
3 of 14
No
No
Hsap\TBX21
3 of 14
No
No
Hsap\TBX22
3 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (17)
Rnor\Tbx20
12 of 14
Yes
Yes
Rnor\Mga
4 of 14
No
Yes
Rnor\Tbxt
4 of 14
No
No
Rnor\Tbx1
3 of 14
No
No
Rnor\Tbx2
3 of 14
No
No
Rnor\Tbx3
3 of 14
No
No
Rnor\Tbx4
3 of 14
No
Yes
Rnor\Tbx5
3 of 14
No
Yes
Rnor\Tbx6
3 of 14
No
No
Rnor\Tbx15
3 of 14
No
No
Rnor\Tbx19
3 of 14
No
No
Rnor\Tbx21
3 of 14
No
No
Rnor\Tbx22
3 of 14
No
No
Rnor\Tbx18
2 of 14
No
No
Rnor\Eomes
1 of 14
No
No
Rnor\Tbr1
1 of 14
No
No
Rnor\Tbx10
1 of 14
No
No
Mus musculus (laboratory mouse) (17)
Mmus\Tbx20
11 of 14
Yes
Yes
Mmus\Mga
4 of 14
No
Yes
Mmus\T
4 of 14
No
No
1  
Mmus\Eomes
3 of 14
No
Yes
Mmus\Tbr1
3 of 14
No
Yes
Mmus\Tbx1
3 of 14
No
No
Mmus\Tbx2
3 of 14
No
No
Mmus\Tbx3
3 of 14
No
No
Mmus\Tbx4
3 of 14
No
Yes
Mmus\Tbx5
3 of 14
No
Yes
Mmus\Tbx6
3 of 14
No
No
Mmus\Tbx15
3 of 14
No
No
Mmus\Tbx19
3 of 14
No
No
Mmus\Tbx21
3 of 14
No
No
Mmus\Tbx22
3 of 14
No
No
Mmus\Tbx10
2 of 14
No
No
Mmus\Tbx18
2 of 14
No
No
Xenopus tropicalis (Western clawed frog) (23)
Xtro\tbx20
8 of 13
Yes
Yes
Xtro\mga
2 of 13
No
No
Xtro\egln2
1 of 13
No
No
Xtro\foxp4
1 of 13
No
No
Xtro\hivep3
1 of 13
No
No
Xtro\nkrf
1 of 13
No
Yes
Xtro\plch1
1 of 13
No
No
Xtro\prnp
1 of 13
No
Yes
Xtro\retsat
1 of 13
No
Yes
Xtro\slc4a10
1 of 13
No
No
Xtro\tbr1
1 of 13
No
No
Xtro\tbx2
1 of 13
No
No
Xtro\tbx4
1 of 13
No
No
Xtro\tbx5
1 of 13
No
No
Xtro\tbx6
1 of 13
No
No
Xtro\tbx15
1 of 13
No
No
Xtro\tbx18
1 of 13
No
No
Xtro\tbx21
1 of 13
No
No
Xtro\tbx22
1 of 13
No
No
Xtro\tbxt.2
1 of 13
No
No
Xtro\tbxt
1 of 13
No
No
Xtro\tef
1 of 13
No
No
Xtro\vegt
1 of 13
No
No
Danio rerio (Zebrafish) (25)
Drer\tbx20
10 of 14
Yes
Yes
Drer\tbx16l
4 of 14
No
No
Drer\tbx19
4 of 14
No
No
Drer\tbxtb
4 of 14
No
No
Drer\eomesa
3 of 14
No
Yes
Drer\mgaa
3 of 14
No
Yes
Drer\tbr1a
3 of 14
No
Yes
Drer\tbr1b
3 of 14
No
Yes
Drer\tbx1
3 of 14
No
No
Drer\tbx2a
3 of 14
No
No
Drer\tbx2b
3 of 14
No
No
Drer\tbx3a
3 of 14
No
No
Drer\tbx3b
3 of 14
No
No
Drer\tbx4
3 of 14
No
Yes
Drer\tbx5a
3 of 14
No
Yes
Drer\tbx6
3 of 14
No
No
Drer\tbx16
3 of 14
No
No
Drer\tbx21
3 of 14
No
Yes
Drer\tbxta
3 of 14
No
No
Drer\eomesb
2 of 14
No
Yes
Drer\tbx5b
2 of 14
No
No
Drer\tbx15
2 of 14
No
No
Drer\tbx22
2 of 14
No
No
Drer\LOC110439164
1 of 14
No
Yes
Drer\tbx18
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (22)
Cele\mab-9
9 of 14
Yes
Yes
Cele\mls-1
2 of 14
No
No
Cele\sea-1
2 of 14
No
No
Cele\tbx-2
2 of 14
No
No
Cele\tbx-7
2 of 14
No
No
Cele\tbx-8
2 of 14
No
No
Cele\tbx-9
2 of 14
No
No
Cele\tbx-11
2 of 14
No
No
Cele\tbx-30
2 of 14
No
No
Cele\tbx-31
2 of 14
No
No
Cele\tbx-32
2 of 14
No
No
Cele\tbx-33
2 of 14
No
No
Cele\tbx-34
2 of 14
No
No
Cele\tbx-35
2 of 14
No
No
Cele\tbx-36
2 of 14
No
No
Cele\tbx-37
2 of 14
No
No
Cele\tbx-38
2 of 14
No
No
Cele\tbx-39
2 of 14
No
No
Cele\tbx-40
2 of 14
No
No
Cele\tbx-41
2 of 14
No
Yes
Cele\tbx-42
2 of 14
No
No
Cele\tbx-43
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (12)
Agam\AgaP_AGAP008569
11 of 12
Yes
Yes
Agam\AgaP_AGAP008563
7 of 12
No
Yes
Agam\AgaP_AGAP008565
6 of 12
No
Yes
Agam\AgaP_AGAP008567
4 of 12
No
No
Agam\AgaP_AGAP005895
3 of 12
No
No
Agam\AgaP_AGAP005896
3 of 12
No
No
Agam\AgaP_AGAP011927
3 of 12
No
No
Agam\AgaP_AGAP001344
2 of 12
No
No
Agam\AgaP_AGAP011711
2 of 12
No
No
Agam\AgaP_AGAP011712
2 of 12
No
No
Agam\AgaP_AGAP008564
1 of 12
No
Yes
Agam\AgaP_AGAP010686
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:mid. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (8)
H15
10 of 13
org-1
5 of 13
bi
4 of 13
byn
4 of 13
Doc1
4 of 13
Doc2
4 of 13
Doc3
4 of 13
ocm
3 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
midRNAi.UAS
model of  cardiomyopathy
CEA
(Qian et al., 2008)
model of  congenital heart disease
CEA
(Qian et al., 2008)
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
TBX20; T-box transcription factor 20
11 of 14
606061
MGA; MAX dimerization protein MGA
4 of 14
616061
    TBX19; T-box transcription factor 19
    4 of 14
    604614
    TBXT; T-box transcription factor T
    4 of 14
    601397
      EOMES; eomesodermin
      3 of 14
      604615
          TBR1; T-box brain transcription factor 1
          3 of 14
          604616
            TBX1; T-box transcription factor 1
            3 of 14
            602054
            TBX2; T-box transcription factor 2
            3 of 14
            600747
             
            TBX3; T-box transcription factor 3
            3 of 14
            601621
             
            TBX4; T-box transcription factor 4
            3 of 14
            601719
            TBX5; T-box transcription factor 5
            3 of 14
            601620
             
            TBX6; T-box transcription factor 6
            3 of 14
            602427
            TBX10; T-box transcription factor 10
            3 of 14
            604648
                TBX15; T-box transcription factor 15
                3 of 14
                604127
                  TBX18; T-box transcription factor 18
                  3 of 14
                  604613
                  TBX21; T-box transcription factor 21
                  3 of 14
                  604895
                  TBX22; T-box transcription factor 22
                  3 of 14
                  300307
                  Functional Complementation Data
                  Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                  Interactions
                  Summary of Physical Interactions
                  esyN Network Diagram
                  Show neighbor-neighbor interactions:
                  Show/hide secondary interactors 
                  (data from AllianceMine provided by esyN)
                  Select Layout:
                  Legend:
                  Protein
                  RNA
                  Selected Interactor(s)
                  Other Interaction Browsers
                  View in FlyBase Interactions Browser   View in MIST tool (external link)
                  Please see the Physical Interaction reports below for full details
                  protein-protein
                  Physical Interaction
                  Assay
                  References
                  mid - Abd-B
                  bimolecular fluorescence complementation, fluorescence microscopy
                  (Baëza et al., 2015)
                  mid - Antp
                  bimolecular fluorescence complementation, fluorescence microscopy
                  (Baëza et al., 2015)
                  mid - B-H2
                  two hybrid array
                  (Shokri et al., 2019)
                  mid - CG15262
                  two hybrid
                  (Hu et al., 2017.6.13)
                  mid - Gsc
                  two hybrid
                  (Hu et al., 2017.6.13)
                  mid - H15
                  two hybrid array
                  (Shokri et al., 2019)
                  mid - Ubx
                  bimolecular fluorescence complementation, fluorescence microscopy
                  (Bischof et al., 2018, Baëza et al., 2015)
                  mid - abd-A
                  bimolecular fluorescence complementation, fluorescence microscopy
                  (Bischof et al., 2018, Baëza et al., 2015)
                  mid - exd
                  bimolecular fluorescence complementation, fluorescence microscopy
                  (Bischof et al., 2018)
                  mid - gro
                  anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, pull down, autoradiography
                  (Formaz-Preston et al., 2012)
                  mid - lms
                  two hybrid
                  (Hu et al., 2017.6.13)
                  mid - otp
                  two hybrid array
                  (Shokri et al., 2019)
                  mid - pnr
                  pull down, autoradiography
                  (Ryu et al., 2011)
                  mid - salm
                  two hybrid array
                  (Shokri et al., 2019)
                  mid - tin
                  pull down, autoradiography
                  (Formaz-Preston et al., 2012, Ryu et al., 2011)
                  Summary of Genetic Interactions
                  esyN Network Diagram
                  Show/hide secondary interactors 
                  (data from AllianceMine provided by esyN)
                  esyN Network Key:
                  Suppression
                  Enhancement
                  Other Interaction Browsers
                  View in FlyBase Interactions Browser   View in MIST tool (external link)
                  Please look at the allele data for full details of the genetic interactions
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  mid
                  enhanceable
                  gro
                  (Formaz-Preston et al., 2012)
                  mid
                  enhanceable
                  H15
                  (Qian et al., 2005)
                  mid
                  enhanceable
                  HGTX
                  (Leal et al., 2009)
                  mid
                  suppressible
                  wg
                  (Gaziova and Bhat, 2009, Buescher et al., 2004)
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  edl
                  suppressible
                  mid
                  (Schwarz et al., 2018)
                  gcm
                  suppressible
                  mid
                  (Popkova et al., 2012)
                  pnr
                  suppressible
                  mid
                  (Qian and Bodmer, 2009)
                  External Data
                  Linkouts
                  DroID - A comprehensive database of gene and protein interactions.
                  MIST (genetic) - An integrated Molecular Interaction Database
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  Pathways
                  Signaling Pathways (FlyBase)
                  Metabolic Pathways
                  FlyBase
                  External Links
                  External Data
                  Linkouts
                  Class of Gene
                  Genomic Location and Detailed Mapping Data
                  Chromosome (arm)
                  2L
                  Recombination map
                  2-17
                  Cytogenetic map
                  25E2-25E2
                  Sequence location
                  FlyBase Computed Cytological Location
                  Cytogenetic map
                  Evidence for location
                  25E2-25E2
                  Limits computationally determined from genome sequence between P{lacW}vrik05901&P{PZ}cype03771 and P{lacW}Hel25Ek11511
                  Experimentally Determined Cytological Location
                  Cytogenetic map
                  Notes
                  References
                  Experimentally Determined Recombination Data
                  Location

                  2-17

                  (FlyBase, 2016)

                  2-18.1

                  (Comeron et al., 2012)

                  2-

                  (Kolodziej et al., 1995)

                  2-16

                  (Tearle and Nusslein-Volhard, 1987)
                  Left of (cM)
                  Right of (cM)
                  (Kotarski et al., 1983)
                  Notes
                  Stocks and Reagents
                  Stocks (31)
                  Genomic Clones (19)
                  cDNA Clones (112)
                   

                  Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                  cDNA clones, fully sequenced
                  BDGP DGC clones
                  Other clones
                    Drosophila Genomics Resource Center cDNA clones

                    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                    cDNA Clones, End Sequenced (ESTs)
                    BDGP DGC clones
                    Other clones
                    RNAi and Array Information
                    Linkouts
                    DRSC - Results frm RNAi screens
                    Antibody Information
                    Laboratory Generated Antibodies
                     

                    polyclonal

                    (Fu et al., 2016, Kumar et al., 2015, Fregoso Lomas et al., 2013, Gaziova and Bhat, 2009, Leal et al., 2009)
                    Commercially Available Antibodies
                     
                    Cell Line Information
                    Publicly Available Cell Lines
                     
                      Other Stable Cell Lines
                       
                        Other Comments

                        mid is required for the formation and specification of neuroblasts in the anterior-most part of each embryonic segment.

                        (Buescher et al., 2006)

                        mid and H15 are negative regulators of wg expression and act to break the symmetry of hh signaling during segmentation of the embryo.

                        (Buescher et al., 2004)

                        Loss of both mid and H15 produces defects that mimic a wg gain-of-function phenotype.

                        (Buescher et al., 2004)

                        Mutants have an extra RP2 or RP2Sib neuron in the endogenous position as well as an ectopic RP2 neuron at the periphery of the nerve cord.

                        (Deshpande and Bhat, 2003)

                        Mutations in 12 complementation groups differentially affect lateral chordotonal axon growth, fasciculation or ventral orientation. Mutations in robo, spen, sli and los cause lch axon defasciculation. Mutations in sli and los also cause some lch axon bundles to grow dorsally along a trajectory 180o from normal.

                        (Kolodziej et al., 1995)

                        mid mutations inhibit the formation of axon tracts between segments of the embryo, sensory axon pathfinding in the PNS and certain tracheal and glial cell migrations.

                        (Lorenz and Perrimon, 1995)

                        Mutations in mid generate malformations of the longitudinal tracts.

                        (Jacobs, 1993)

                        The role of mid in the regulation of run mRNA expression in the early embryo has been investigated.

                        (Klingler and Gergen, 1993)
                        Relationship to Other Genes
                        Source for database merge of

                        Source for merge of: nmr2 mid

                        (Reim et al., 2005)

                        Source for merge of: mid nmr2

                        (Buescher et al., 2004)

                        Source for merge of: mid los extra

                        (Gaziova and Bhat, 2009)
                        Additional comments
                        Nomenclature History
                        Source for database identify of

                        Source for identity of: nmr2 CG6634

                        (Qian, 2004.2.25)
                        Nomenclature comments
                        Etymology
                        Synonyms and Secondary IDs (19)
                        Reported As
                        Symbol Synonym
                        CG6634
                        (National Institute of Genetics Fly Stocks, 2022-, Frise et al., 2010, Ni et al., 2010.12.1, Hazelett et al., 2009, Keleman et al., 2009.8.5, Liu et al., 2009, Copley, 2005, Buescher et al., 2004, Prpic et al., 2003, Brody et al., 2002, Halfon et al., 2002)
                        H15
                        (Pyrowolakis et al., 2017)
                        H15r
                        (Reim et al., 2003)
                        H15r/nmr2
                        (Reim et al., 2005)
                        MID
                        (Pyrowolakis et al., 2017)
                        Mid
                        (Poovathumkadavil and Jagla, 2020, Svendsen et al., 2019, Bischof et al., 2018, Baëza et al., 2015, Ferrero et al., 2014)
                        Mid(H15)
                        (Davidson and Erwin, 2006)
                        TBX20
                        (Amodio et al., 2012)
                        extra
                        los
                        (Gaziova and Bhat, 2009)
                        mid
                        (Grunert et al., 2024, Makhijani et al., 2024, Miao et al., 2024, Zhang et al., 2024, Huang et al., 2023, Sun et al., 2023, Voutyraki et al., 2023, Junion and Jagla, 2022, Katti et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Bilder et al., 2021, Boukhatmi, 2021, Everetts et al., 2021, McLaughlin et al., 2021, Piedade and Famulski, 2021, Ruiz-Losada et al., 2021, Slaidina et al., 2021, Gaziova et al., 2020, Lepesant et al., 2020, Rust et al., 2020, Shokri et al., 2019, Wittes and Schüpbach, 2019, Schwarz et al., 2018, Hu et al., 2017.6.13, Karaiskos et al., 2017, Osterfield et al., 2017, Revaitis et al., 2017, Field et al., 2016, Fregoso Lomas et al., 2016, Sarov et al., 2016, Chen et al., 2015, Kumar et al., 2015, Svendsen et al., 2015, Fauré et al., 2014, Tripathy et al., 2014, Zarin et al., 2014, Das et al., 2013, Fregoso Lomas et al., 2013, Jin et al., 2013, Manavalan et al., 2013, Terriente-Felix et al., 2013, Amodio et al., 2012, Formaz-Preston et al., 2012, Popkova et al., 2012, Johnson et al., 2011, Ryu et al., 2011, Kazemian et al., 2010, Reim and Frasch, 2010, Gaziova and Bhat, 2009, Leal et al., 2009, Liu et al., 2009, Liu et al., 2009, Svendsen et al., 2009, Wheeler et al., 2009, Engström et al., 2007, Johnson et al., 2007, Davidson and Erwin, 2006, Zaffran et al., 2006)
                        nmr2
                        (Lacin et al., 2014, Buffin and Gho, 2010, Leal et al., 2009, Qian and Bodmer, 2009, Qian et al., 2008, Sandmann et al., 2006, Qian et al., 2005, Qian, 2004.2.25, Qian et al., 2004)
                        Name Synonyms
                        H15-related
                        (Reim et al., 2003)
                        Midline
                        (Svendsen et al., 2019, Kumar et al., 2015)
                        extra
                        (Gaziova and Bhat, 2009, Deshpande and Bhat, 2003)
                        lost in space
                        (Gaziova and Bhat, 2009, Kolodziej et al., 1995)
                        midline
                        (Osterfield et al., 2017, Wieschaus and Nüsslein-Volhard, 2016, Baëza et al., 2015, Chen et al., 2015, Svendsen et al., 2015, Das et al., 2013, Fregoso Lomas et al., 2013, Amodio et al., 2012, Formaz-Preston et al., 2012, Johnson et al., 2011, Ryu et al., 2011, Carpenter et al., 2009, Gaziova and Bhat, 2009, Hazelett et al., 2009, Leal et al., 2009, Liu et al., 2009, Svendsen et al., 2009, Qian et al., 2008, Johnson et al., 2007, Buescher et al., 2006)
                        neuromancer 1
                        (Ryu et al., 2011)
                        neuromancer 2
                        (Svendsen et al., 2009, Qian et al., 2005)
                        neuromancer2
                        (Buffin and Gho, 2010, Leal et al., 2009, Qian et al., 2004)
                        Secondary FlyBase IDs
                        • FBgn0005511
                        • FBgn0001934
                        • FBgn0002746
                        • FBgn0031706
                        • FBgn0016996
                        • FBgn0065989
                        Datasets (0)
                        Study focus (0)
                        Experimental Role
                        Project
                        Project Type
                        Title
                        Study result (0)
                        Result
                        Result Type
                        Title
                        External Crossreferences and Linkouts ( 37 )
                        Sequence Crossreferences
                        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                        Other crossreferences
                        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                        InterPro - A database of protein families, domains and functional sites
                        KEGG Genes - Molecular building blocks of life in the genomic space.
                        MARRVEL_MODEL - MARRVEL (model organism gene)
                        Linkouts
                        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                        DroID - A comprehensive database of gene and protein interactions.
                        DRSC - Results frm RNAi screens
                        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                        MIST (genetic) - An integrated Molecular Interaction Database
                        MIST (protein-protein) - An integrated Molecular Interaction Database
                        References (140)