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Gene Dmel\mid

General Information
Symbol	Dmel\mid	Species	D. melanogaster
Name	midline	Annotation symbol	CG6634
Feature type	protein_coding_gene	FlyBase ID	FBgn0261963
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	nmr2
Genomic Location
Chromosome (arm)	2L	Recombination map	2-16
Cytogenetic map	25E2-25E2	Sequence location	2L:5,461,641..5,467,610 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene midline is referred to in FlyBase by the symbol Dmel\mid (CG6634, FBgn0261963). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: sequence-specific DNA binding transcription factor activity. There is experimental evidence for 19 unique biological process terms, many of which group under: system development; biological regulation; cell fate commitment; cardiovascular system development; neuron differentiation; multicellular organismal development; regulation of developmental process; cellular component organization or biogenesis; neuroblast differentiation; regionalization. 17 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; embryonic/larval neuron; adult segment; ventral nerve cord primordium; acellular anatomical structure; embryonic/larval dorsal vessel; portion of tissue; peripheral nervous system; non-connected developing system; appendage segment. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Transcription factor, T-box; Transcription factor, T-box, conserved site; p53-like transcription factor, DNA-binding. Gene sequence location is 2L:5461641..5467610.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	mid: midline Homozygous embryonic lethal; denticle bands defective in ventral midline.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	Controlled Vocabulary Terms neuroblast NB7-1 neuroblast NB7-2 neuroblast NB6-2 neuroblast NB2-5 ventral midline dorsal closure stage RP2 neuron late extended germ band stage neuroblast NB1-2 embryonic stage 11 neuroblast NB6-4 neuroblast NB7-4 embryonic stage 13 embryonic stage 10 neuroblast NB3-2 embryonic stage 16 Transgenic Constructs P{mid-lacZ.1.7.Pelican} P{mid-lacZ.0.18.Pelican} P{mid-lacZ.0.7.Pelican} P{mid-lacZ.0.3mut.Pelican} P{mid-lacZ.1T.Pelican} P{mid-lacZ.0.18mut.Pelican} P{mid-lacZ.2.6.Pelican} P{mid-lacZ.1.0mut.Pelican} P{mid-lacZ.0.3.Pelican} P{mid-lacZ.2T2P.Pelican} P{mid-lacZ.5.7.Pelican} P{mid-lacZ.4.3.Pelican} P{mid-lacZ.1.0.Pelican} P{mid-lacZ.1T1P.Pelican} Transcripts mid-RB Polypeptides mid-PB
FB2012_01	References Kazemian et al., 2010, PLoS Biol. 8(8): Sequence features BKN21223 HFA02977 Controlled Vocabulary Terms positive regulation of transcription from RNA polymerase II promoter
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 25E2-25E2   Limits computationally determined from genome sequence between P{lacW}vrik05901&P{PZ}cype03771 and P{lacW}Hel25Ek11511
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	2-16 (Tearle and Nusslein-Volhard, 1987) 2- (Kolodziej et al., 1995)
Left of (cM)
Right of (cM)	Hel25E (Kotarski et al., 1983)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mid for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mid-RA FBtr0079093  NM_135083   2918   580 mid-RB FBtr0310358     2137   580
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mid-PA   FBpp0078726   62.9   580   8.31     NP_608927   AAF52250 mid-PB   FBpp0302039   62.9   580   8.31
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor, T-box, conserved site (IPR018186) Transcription factor, T-box (IPR001699) p53-like transcription factor, DNA-binding (IPR008967)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF52250   AY071236 AAL48858   BI163492   BI168919   BI214964   BI229620   BI369038   BT023769   CO176893   CO178037   CO178143   CO186694   CO285579   CO287298   CO301119   CO304715   CO326626   EC057334   EC068882   EC069723   EC070932   EC207206   EC211329   EC249052   EC253115   GH748787   GH754328   GH754973   GH756570   GH756886   GH756930   GH759003   GH760710   GH773637   GH773812   GH774964   GH775486   GH776451   GH777228   GH778062   GH780143   GH781055   GH784348   GH784730   GH786397   GH786864   GH786917   GH787998   GH789190   GH789587   GH790678   GH792296   GH792735   GH793265   GH797161   GH799730   GH801343   GH802932   GH806402   GH807087   GH807258   GH810271   GH811341   GH811383   GH812186   GH815866   GH819767   GH819982   GH824279   GH825864   GH826558   GH828868   GH829878   GH830057   GH833241   GH834315   GH834385   GH838525   GH839093   GH843113   GH844724   GH846470   GH849089   GH854712   GH856923   GH863319   GH869014   GH869324   GH872888   GH872896   GH879995   GH881409   GH881540   GH883598   GH883841   GH884887   GH885559   GH886419   GH890877   GH894873   GH896543   GH897393   GH899005   GH904151   GH907995   GH908272   GH908968   GH911225   GH914689   GH920530   GH921496   GH925842   GH927390   GH928701   GH932918   GH934363   GH935311   GH935610   GH937418   GH939502   GH939735   GH940189
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8SYY8 Q9VMR2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5   organism | segment polarity expression pattern   (Prpic et al., 2003) embryonic stage 12   embryonic ventral nervous system   (Prpic et al., 2003) embryonic stage 14 -- 15   heart primordium   (Prpic et al., 2003) embryonic stage 15   embryonic brain   (Prpic et al., 2003) third instar larval stage   ventral thoracic disc | proximal & ventral   (Prpic et al., 2003)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 10   neuroblast NB7-1   (Gaziova and Bhat, 2009) neuroblast NB7-2   (Gaziova and Bhat, 2009) neuroblast NB3-2   (Gaziova and Bhat, 2009) embryonic stage 10 -- 11   neuroblast NB7-4   (Gaziova and Bhat, 2009) neuroblast NB2-5   (Gaziova and Bhat, 2009) embryonic stage 10 -- 16   ventral midline   (Gaziova and Bhat, 2009) embryonic stage 11   neuroblast NB1-2   (Gaziova and Bhat, 2009) neuroblast NB6-2   (Gaziova and Bhat, 2009) neuroblast NB6-4   (Gaziova and Bhat, 2009) embryonic stage 13 -- 16   RP2 neuron   (Gaziova and Bhat, 2009)
Additional Descriptive Data
	mid protein is expressed in a large number of neurons in the ventral midline. It was localized to specific midline neuroblasts at embryonic stages 10 and 11. At stage 13, it was found to be expressed in a row 5 GMC, dubbed an "M-neuron", that appears to change into an extra RP2. It's relation to the NB4-2->GMC-1->RP2/sib lineage is described. (Gaziova and Bhat, 2009)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_31
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage mid1 lethal | embryonic stage | recessive midunspecified viable, with Scer\GAL4pnr-MD237 midGD4292 Other Phenotypes Allele cell polarity defective, with Scer\GAL4tin.CΔ4 middsRNA.Scer\UAS neuroanatomy defective mid1 midGA174 neuroanatomy defective (with Df(2L)x528) mid1 midGA174 neuroanatomy defective | embryonic stage mid1 mid2 midD62 midlos1 neuroanatomy defective | embryonic stage (with Df(2L)Exel6012) mid1 mid2 midlos1 neuroanatomy defective | embryonic stage (with mid1) mid2 midlos1 neuroanatomy defective | embryonic stage (with mid2) mid1 midlos1 neuroanatomy defective | embryonic stage (with midlos1) mid1 mid2 small body | larval stage, with Scer\GAL4ptc-559.1 midScer\UAS.cBa visible | male limited, with Scer\GAL4bi-omb-Gal4 midScer\UAS.cBa Phenotype manifest in Allele cardioblast mid1 cardioblast, with Scer\GAL4how-24B, Scer\GAL4twi.PG midScer\UAS.cRa middsRNA.Scer\UAS cardioblast, with Scer\GAL4tin.CΔ4 middsRNA.Scer\UAS cardioblast (with Df(2L)cl-h2) mid2 cardioblast | ectopic, with Scer\GAL4how-24B midScer\UAS.cBa chordotonal organ & axon | lateral midlos1 commissure midlos1 denticle | ectopic, with Scer\GAL4ptc-559.1 midScer\UAS.cBa denticle | ectopic, with Scer\GAL4sca-P309 midScer\UAS.cBa denticle belt midGA174 mid1 denticle belt, with Scer\GAL4prd.RG1 midScer\UAS.cBa denticle row 1 & abdominal 1 ventral denticle belt midunspecified denticle row 1 & abdominal 2 ventral denticle belt midunspecified denticle row 1 & abdominal 3 ventral denticle belt midunspecified denticle row 1 & abdominal 4 ventral denticle belt midunspecified denticle row 1 & abdominal 5 ventral denticle belt midunspecified denticle row 1 & abdominal 6 ventral denticle belt midunspecified denticle row 1 & abdominal 7 ventral denticle belt midunspecified denticle row 1 & abdominal 8 ventral denticle belt midunspecified denticle row 2 & abdominal 1 ventral denticle belt midunspecified denticle row 2 & abdominal 2 ventral denticle belt midunspecified denticle row 2 & abdominal 3 ventral denticle belt midunspecified denticle row 2 & abdominal 4 ventral denticle belt midunspecified denticle row 2 & abdominal 5 ventral denticle belt midunspecified denticle row 2 & abdominal 6 ventral denticle belt midunspecified denticle row 2 & abdominal 7 ventral denticle belt midunspecified denticle row 2 & abdominal 8 ventral denticle belt midunspecified denticle row 3 & abdominal 1 ventral denticle belt midunspecified denticle row 3 & abdominal 2 ventral denticle belt midunspecified denticle row 3 & abdominal 3 ventral denticle belt midunspecified denticle row 3 & abdominal 4 ventral denticle belt midunspecified denticle row 3 & abdominal 5 ventral denticle belt midunspecified denticle row 3 & abdominal 6 ventral denticle belt midunspecified denticle row 3 & abdominal 7 ventral denticle belt midunspecified denticle row 3 & abdominal 8 ventral denticle belt midunspecified dorsal vessel primordium, with Scer\GAL4how-24B, Scer\GAL4twi.PG midScer\UAS.cRa embryonic/first instar larval cuticle mid3 embryonic/larval cuticle, with Scer\GAL4ptc-559.1 midScer\UAS.cBa embryonic/larval cuticle, with Scer\GAL4sca-P309 midScer\UAS.cBa embryonic/larval dorsal vessel, with Scer\GAL4pnr-MD237 midScer\UAS.cBa embryonic/larval dorsal vessel, with Scer\GAL4twi.PB midScer\UAS.cBa embryonic/larval heart mid1 embryonic/larval heart, with Scer\GAL4how-24B, Scer\GAL4twi.PG middsRNA.Scer\UAS embryonic/larval heart, with Scer\GAL4pnr-MD237 midScer\UAS.cBa embryonic/larval heart, with Scer\GAL4twi.PB midScer\UAS.cBa embryonic/larval ostia mid1 embryonic/larval ostia (with Df(2L)cl-h2) mid2 embryonic/larval pericardial cell mid1 ganglion mother cell GMC1-1a midGA174 larval head, with Scer\GAL4ptc-559.1 midScer\UAS.cBa lateral cord neuron midGA174 longitudinal connective midlos1 longitudinal connective | embryonic stage mid1 mesothoracic tarsal bristle, with Scer\GAL4Act5C.PI midScer\UAS.cBa mesothoracic tarsal bristle longitudinal row 1 | ectopic | somatic clone, with Scer\GAL4Act5C.PI midScer\UAS.cBa mesothoracic tarsal bristle longitudinal row 8 | ectopic | somatic clone, with Scer\GAL4Act5C.PI midScer\UAS.cBa mesothoracic tibial apical bristle | ectopic, with Scer\GAL4bi-omb-Gal4 midScer\UAS.cBa mesothoracic tibial apical bristle | ectopic | somatic clone, with Scer\GAL4Act5C.PI midScer\UAS.cBa mesothoracic tibial spur bristle | ectopic, with Scer\GAL4bi-omb-Gal4 midScer\UAS.cBa mesothoracic tibial spur bristle | ectopic | somatic clone, with Scer\GAL4Act5C.PI midScer\UAS.cBa metathoracic tarsal bristle, with Scer\GAL4Act5C.PI midScer\UAS.cBa motor neuron midD62 mid2 neuroblast NB1-1 of abdomen midGA174 mid1 neuroblast NB1-1 of abdomen (with Df(2L)x528) midGA174 mid1 neuroblast NB2-4 of abdomen midGA174 neuroblast NB2-4 of abdomen (with Df(2L)x528) midGA174 neuroblast NB2-5 midGA174 neuroblast NB2-5 (with Df(2L)x528) midGA174 neuron | supernumerary midGA174 posterior commissure | embryonic stage mid1 presumptive embryonic/larval central nervous system midGA174 prothoracic tarsal bristle, with Scer\GAL4Act5C.PI midScer\UAS.cBa prothoracic tibial transverse bristle row | ectopic, with Scer\GAL4bi-omb-Gal4 midScer\UAS.cBa RP1 neuron mid1 RP2 neuron | ectopic mid1 mid2 midlos1 RP2 neuron | ectopic (with Df(2L)Exel6012) mid1 mid2 midlos1 RP2 neuron | ectopic (with mid1) mid2 midlos1 RP2 neuron | ectopic (with mid2) mid1 midlos1 RP2 neuron | ectopic (with midlos1) mid1 mid2 RP3 neuron mid1 sensory neuron | embryonic stage mid1 sex comb | ectopic, with Scer\GAL4bi-omb-Gal4 midScer\UAS.cBa
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of mid Class Mutagen Stocks Known lesion mid1 amorphic allele - genetic evidence, hypomorphic allele - genetic evidence ethyl methanesulfonate 2 Yes mid2 amorphic allele - genetic evidence, hypomorphic allele - genetic evidence ethyl methanesulfonate 2 Yes midlos1 hypomorphic allele - genetic evidence diepoxybutane 1 Yes midGA174 loss of function allele, amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes mid1a5 ethyl methanesulfonate 0 Yes mid3 ethyl methanesulfonate 0 -- mid8 X ray 0 -- midD62 0 Yes midunspecified 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mid Class Mutagen Stocks Known lesion midGD4292 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes midKK101127 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes middsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes midScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes midScer\UAS.cGa in vitro construct - regulatory fusion 0 Yes midScer\UAS.cLa in vitro construct - regulatory fusion 0 Yes midScer\UAS.cQb in vitro construct - regulatory fusion 0 Yes midScer\UAS.cRa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)cl1 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Tearle and Nusslein-Volhard, 1987, Szidonya and Reuter, 1988) Df(2L)cl2 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)cl7 (Szidonya and Reuter, 1988, Kotarski et al., 1983, Tearle and Nusslein-Volhard, 1987, Szidonya and Reuter, 1988) Df(2L)cl-h1 (Szidonya and Reuter, 1988, Kotarski et al., 1983, Szidonya and Reuter, 1988) Df(2L)cl-h2 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Kotarski et al., 1983) Df(2L)cl-h3 (Szidonya and Reuter, 1988, Harbecke and Lengyel, 1995, Szidonya and Reuter, 1988, Kotarski et al., 1983) Df(2L)Exel6012 (Ryder, 2004.4.26) Df(2L)GpdhA (Harbecke and Lengyel, 1995, Tearle and Nusslein-Volhard, 1987, Buescher et al., 2004, Miskolczi-McCallum et al., 2005) Df(2L)odd-4.25 (Nusslein-Volhard et al., 1984) Df(2L)sc19-4 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Leal et al., 2009) Df(2L)sc19-12 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)x528 (Buescher et al., 2004, Leal et al., 2009, Miskolczi-McCallum et al., 2005)
Not disrupted in	Df(2L)cl-h4 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)H15-x4 (Miskolczi-McCallum et al., 2005) Df(2L)sc19-1 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-5 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-6 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-7 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-8 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)tkv2 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)tkv3 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Duplicated in	Dp(2;1)B19
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{mid-lacZ.0.3.Pelican} No P{mid-lacZ.0.3mut.Pelican} No P{mid-lacZ.0.7.Pelican} No P{mid-lacZ.0.18.Pelican} No P{mid-lacZ.0.18mut.Pelican} No P{mid-lacZ.1.0.Pelican} No P{mid-lacZ.1.0mut.Pelican} No P{mid-lacZ.1.7.Pelican} No P{mid-lacZ.1T1P.Pelican} No P{mid-lacZ.1T.Pelican} No P{mid-lacZ.2.6.Pelican} No P{mid-lacZ.2T2P.Pelican} No P{mid-lacZ.4.3.Pelican} No P{mid-lacZ.5.7.Pelican} No UAS construct P{GD4292} NA P{KK101127} NA P{UAS-mid.B} NA P{UAS-mid.dsRNA} NA P{UAS-mid.L} NA P{UAS-mid.Q} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 22 unique terms )
Terms Based on Experimental Evidence ( 19 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Liu et al., 2009) axonogenesis inferred from mutant phenotype (Kolodziej et al., 1995) cardioblast cell fate commitment inferred from mutant phenotype (Qian et al., 2005) central nervous system development inferred from genetic interaction with HGTX (Leal et al., 2009) inferred from mutant phenotype (Leal et al., 2009) embryonic heart tube development inferred from mutant phenotype (Qian et al., 2005) ganglion mother cell fate determination inferred from mutant phenotype (Gaziova and Bhat, 2009) heart development inferred from mutant phenotype (Miskolczi-McCallum et al., 2005) heart process inferred from direct assay (Qian et al., 2008) leg disc pattern formation inferred from genetic interaction with H15 (Svendsen et al., 2009) negative regulation of gene expression inferred from genetic interaction with HGTX (Leal et al., 2009) inferred from mutant phenotype (Leal et al., 2009) nervous system development inferred from mutant phenotype (Deshpande and Bhat, 2003) neuroblast development inferred from mutant phenotype (Buescher et al., 2006) neuroblast fate specification inferred from mutant phenotype (Buescher et al., 2006) neuron fate specification inferred from mutant phenotype (Gaziova and Bhat, 2009) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Gaziova and Bhat, 2009) regulation of cardioblast differentiation inferred from mutant phenotype (Reim et al., 2005) regulation of cell fate specification inferred from genetic interaction with H15 (Svendsen et al., 2009) regulation of nervous system development inferred from mutant phenotype (Gaziova and Bhat, 2009) segment polarity determination inferred from mutant phenotype (Tearle and Nusslein-Volhard, 1987)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding transcription factor activity inferred from sequence or structural similarity with EMBL:AC006379 (FlyBase, 1992-)
Biological Process
CV term Evidence References
axonogenesis non-traceable author statement (Tear, 1999) regulation of transcription, DNA-dependent inferred from sequence or structural similarity with EMBL:AC006379 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with EMBL:AC006379 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mid allele Gene References middsRNA.Scer\UAS H15 (Qian et al., 2005) midScer\UAS.cBa wg (Buescher et al., 2004) unspecified pnr (Qian et al., 2005)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261963 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33770
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14392 Dere\GG25073 Dsec\GM18548 Dyak\GE25417
Curated drosophilid orthologs	Dpse\GA19742
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	8789 w*; midlos1 P{neoFRT}40A/SM6b 18 mid2 cn1 bw1 sp1/CyO 3086 mid1 cn1 bw1 sp1/CyO
Kyoto	105679 mid2 cn1 bw1 sp1/CyO 106830 mid1 cn1 bw1 sp1/CyO
VDRC	v105411 P{KK101127}VIE-260B v30519 w1118; P{GD4292}v30519/TM3
Genomic Clones ( 1 )
	BACR44J04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 112 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP04777 RE27439
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE02909 RE21133 RE55119
Other clones	EK022230 EK051516 EK055045 EK055510 EK065510 EK169508 EK180163 EK245851 EP04332 RE09827 RP003026784 RP003038019 RP003040036 RP003068537 RP003080971 RP003093938 RP003101963 RP003102770 RP003106509 RP003109251 RP003110905 RP003118316 RP003121503 RP003135888 RP003137405 RP003145169 RP003148281 RP003166144 RP003174304 RP003176626 RP003177806 RP003184315 RP003194005 RP003207057 RP003207142 RP003222144 RP003222829 RP003226657 RP003237450 RP003246011 RP003248082 RP003251183 RP003254521 RP003259871 RP003260641 RP003281970 RP003288776 RP003289622 RP003297666 RP003299731 RP003305110 RP003307232 RP003308330 RP003316505 RP003339441 RP003344625 RP003361968 RP003380324 RP003381504 RP003391369 RP003398726 RP003411625 RP003412587 RP003416535 RP003417415 RP003424140 RP003425244 RP003429011 RP003434018 RP003434926 RP003436764 RP003445202 RP003447867 RP003454082 RP003469088 RP003472822 RP003479081 RP003487291 RP003489355 RP003492078 RP003492489 RP003502704 RP003505736 RP003509959 RP003515786 RP003515965 RP003517154 RP003522310 RP003533219 RP003537813 RP003539897 RP003556885 RP003561157 RP003573990 RP003584758 RP003595778 RP003619599 RP003625533 RP003630114 RP003635371 RP003637034 RP003641802 RP003650934 RP003652665 RP003655003 RP003664932 RP003667528
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261963 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33770 44899 5657493
Antibody Information
	polyclonal (Leal et al., 2009, Gaziova and Bhat, 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: nmr2 CG6634 (Qian, 2004.2.25)
Source for database merge of	Source for merge of: mid los extra (Gaziova and Bhat, 2009) Source for merge of: mid nmr2 (Buescher et al., 2004) Source for merge of: nmr2 mid (Reim et al., 2005)
Additional comments
Other Comments
	mid is required for the formation and specification of neuroblasts in the anterior-most part of each embryonic segment. (Buescher et al., 2006) mid and H15 are negative regulators of wg expression and act to break the symmetry of hh signaling during segmentation of the embryo. (Buescher et al., 2004) Loss of both mid and H15 produces defects that mimic a wg gain-of-function phenotype. (Buescher et al., 2004) Mutants have an extra RP2 or RP2Sib neuron in the endogenous position as well as an ectopic RP2 neuron at the periphery of the nerve cord. (Deshpande and Bhat, 2003) Mutations in 12 complementation groups differentially affect lateral chordotonal axon growth, fasciculation or ventral orientation. Mutations in robo, spen, sli and los cause lch axon defasciculation. Mutations in sli and los also cause some lch axon bundles to grow dorsally along a trajectory 180o from normal. (Kolodziej et al., 1995) mid mutations inhibit the formation of axon tracts between segments of the embryo, sensory axon pathfinding in the PNS and certain tracheal and glial cell migrations. (Lorenz and Perrimon, 1995) Mutations in mid generate malformations of the longitudinal tracts. (Jacobs, 1993) The role of mid in the regulation of run mRNA expression in the early embryo has been investigated. (Klingler and Gergen, 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135083 RefSeq (Proteins) • NP_608927 DDBJ / EMBL / GenBank • AAF52250 AAL48858 AY071236 BI229620 Entrez Gene - A searchable database of RefSeq genes. • 33770 UniProtKB/TrEMBL • Q8SYY8 Q9VMR2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Transcription factor, T-box, conserved site (IPR018186) Transcription factor, T-box (IPR001699) p53-like transcription factor, DNA-binding (IPR008967)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261963 DRSC - Results from RNAi screens. • FBgn0261963 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261963 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33770 44899 5657493 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33770 modMine - Data generated by the modENCODE project. • FBgn0261963
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	CG6634 (Buescher et al., 2004, Prpic et al., 2003, Halfon et al., 2002, Brody et al., 2002, Liu et al., 2009, Keleman et al., 2009.8.5, Hazelett et al., 2009) extra   H15r (Reim et al., 2003) H15r/nmr2 (Reim et al., 2005) los (Gaziova and Bhat, 2009) mid (Engström et al., 2007, Zaffran et al., 2006, Davidson and Erwin, 2006, Johnson et al., 2007, Liu et al., 2009, Leal et al., 2009, Gaziova and Bhat, 2009, Liu et al., 2009, Svendsen et al., 2009, Wheeler et al., 2009, Ryu et al., 2011, Kazemian et al., 2010) Mid(H15) (Davidson and Erwin, 2006) nmr2 (Qian et al., 2005, Qian, 2004.2.25, Qian et al., 2004, Sandmann et al., 2006, Leal et al., 2009, Qian and Bodmer, 2009, Buffin and Gho, 2010, Qian et al., 2008)
Name Synonym	extra (Deshpande and Bhat, 2003, Gaziova and Bhat, 2009) H15-related (Reim et al., 2003) lost in space (Kolodziej et al., 1995, Gaziova and Bhat, 2009) midline (Buescher et al., 2006, Johnson et al., 2007, Gaziova and Bhat, 2009, Liu et al., 2009, Svendsen et al., 2009, Carpenter et al., 2009, Leal et al., 2009, Qian et al., 2008, Hazelett et al., 2009, Ryu et al., 2011) neuromancer 1 (Ryu et al., 2011) neuromancer 2 (Qian et al., 2005, Svendsen et al., 2009) neuromancer2 (Qian et al., 2004, Leal et al., 2009, Buffin and Gho, 2010)
Secondary FlyBase IDs
	FBgn0001934 FBgn0002746 FBgn0005511 FBgn0016996 FBgn0031706 FBgn0065989
References ( 67 )
Generate a list of	All references relating to this gene ( 67 )
List References by type	Research paper  ( 37 ) Supplementary material  ( 2 ) Review  ( 5 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 11 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 3 )
	Ryu et al., 2011, Dev. Dyn. 240(1): 86--96 Tinman is a direct activator of midline in the drosophila dorsal vessel. [FBrf0212547] Buffin and Gho, 2010, PLoS ONE 5(2): e9285 Laser microdissection of sensory organ precursor cells of Drosophila microchaetes. [FBrf0210039] Kazemian et al., 2010, PLoS Biol. 8(8): Quantitative analysis of the Drosophila segmentation regulatory network using pattern generating potentials. [FBrf0211688] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...