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Gene Dmel\Ire1

General Information
Symbol	Dmel\Ire1	Species	D. melanogaster
Name	Inositol-requiring enzyme-1	Annotation symbol	CG4583
Feature type	protein_coding_gene	FlyBase ID	FBgn0261984
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	ire-1, ire1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	92B3-92B7	Sequence location	3R:15,679,630..15,687,013 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Inositol-requiring enzyme-1 is referred to in FlyBase by the symbol Dmel\Ire1 (CG4583, FBgn0261984). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: endoribonuclease activity. There is experimental evidence that it is involved in the biological process: endoplasmic reticulum unfolded protein response; Golgi organization; mRNA catabolic process; response to endoplasmic reticulum stress; HAC1-type intron splice site recognition and cleavage; regulation of RNA splicing. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: KEN domain; PUG domain; Protein kinase, catalytic domain; Protein kinase-like domain; Pyrrolo-quinoline quinone beta-propeller repeat; Quinonprotein alcohol dehydrogenase-like-superfamily; Serine/threonine-protein kinase, active site. Gene sequence location is 3R:15679630..15687013.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 92B3-92B7   Limits computationally determined from genome sequence between P{EP}EP650 and P{PZ}l(3)1058510585; Left limit from (method unavailable) (FBrf0126876) Right limit from (method unavailable) (FBrf0126876)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 92B1-92B7     (Cavener et al., 2000.3.10)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Ire1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Ire1-RB FBtr0113248  NM_001104369   4034   1074
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Ire1-PB   FBpp0112160   120.2   1074   6.49     NP_001097839   ABW08704
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Pyrrolo-quinoline quinone beta-propeller repeat (IPR018391) Quinonprotein alcohol dehydrogenase-like domain (IPR027295) Protein kinase, catalytic domain (IPR000719) PUG domain (IPR006567) Serine/threonine-protein kinase, active site (IPR008271) KEN domain (IPR010513) Protein kinase-like domain (IPR011009) Quinonprotein alcohol dehydrogenase-like superfamily (IPR011047)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 ABW08704   BT099629   CO279704   CO299214   CO307354   CO330772   CZ484678   EC053542   EC056199   EC057163   EC216354   EC226464   EC239665   EV592721   GH792393   GH970352
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A8JR46 A8JR47
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    10 central nervous system, pupae P8    16 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    12 head, mated 4-day male    16 head, mated 20-day male    20 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    12 digestive system, 4-day adult    12 digestive system, 20-day adult    11 fat body, larvae L3 wandering    7 fat body, white prepupae    13 fat body, pupae P8    13 carcass, larvae L3 wandering    11 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    10 ovary, virgin 4-day female    29 ovary, mated 4-day female    24 testis, mated 4-day male    6 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    10 central nervous system, pupae P8    16 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    12 head, mated 4-day male    16 head, mated 20-day male    20 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae  (44) digestive system, larvae L3 wandering    13 digestive system, 1-day adult    12 digestive system, 4-day adult    12 digestive system, 20-day adult    11 fat body, larvae L3 wandering    7 fat body, white prepupae    13 fat body, pupae P8    13 carcass, larvae L3 wandering    11 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    10 ovary, virgin 4-day female  (29) ovary, mated 4-day female    24 testis, mated 4-day male    6 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    10 central nervous system, pupae P8    16 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    12 head, mated 4-day male    16 head, mated 20-day male    20 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    12 digestive system, 4-day adult    12 digestive system, 20-day adult    11 fat body, larvae L3 wandering    7 fat body, white prepupae    13 fat body, pupae P8    13 carcass, larvae L3 wandering    11 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    10 ovary, virgin 4-day female    29 ovary, mated 4-day female    24 testis, mated 4-day male    6 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    10 central nervous system, pupae P8    16 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    12 head, mated 4-day male    16 head, mated 20-day male    20 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    12 digestive system, 4-day adult    12 digestive system, 20-day adult    11 fat body, larvae L3 wandering    7 fat body, white prepupae    13 fat body, pupae P8    13 carcass, larvae L3 wandering    11 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    10 ovary, virgin 4-day female    29 ovary, mated 4-day female    24 testis, mated 4-day male    6 accessory gland, mated 4-day male    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    10 central nervous system, pupae P8    16 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    12 head, mated 4-day male    16 head, mated 20-day male    20 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    12 digestive system, 4-day adult    12 digestive system, 20-day adult    11 fat body, larvae L3 wandering    7 fat body, white prepupae    13 fat body, pupae P8    13 carcass, larvae L3 wandering    11 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    10 ovary, virgin 4-day female    29 ovary, mated 4-day female    24 testis, mated 4-day male    6 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    10 central nervous system, pupae P8    16 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    12 head, mated 4-day male    16 head, mated 20-day male    20 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae  (44) digestive system, larvae L3 wandering    13 digestive system, 1-day adult    12 digestive system, 4-day adult    12 digestive system, 20-day adult    11 fat body, larvae L3 wandering    7 fat body, white prepupae    13 fat body, pupae P8    13 carcass, larvae L3 wandering    11 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    10 ovary, virgin 4-day female  29 ovary, mated 4-day female    24 testis, mated 4-day male    6 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    10 central nervous system, pupae P8    16 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    12 head, mated 4-day male    16 head, mated 20-day male    20 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    12 digestive system, 4-day adult    12 digestive system, 20-day adult    11 fat body, larvae L3 wandering    7 fat body, white prepupae    13 fat body, pupae P8    13 carcass, larvae L3 wandering    11 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    10 ovary, virgin 4-day female    29 ovary, mated 4-day female    24 testis, mated 4-day male    6 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    12 central nervous system, larvae L3    10 central nervous system, pupae P8    16 head, virgin 1-day female    11 head, virgin 4-day female    10 head, virgin 20-day female    16 head, mated 1-day female    10 head, mated 4-day female    14 head, mated 20-day female    11 head, mated 1-day male    12 head, mated 4-day male    16 head, mated 20-day male    20 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    44 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    12 digestive system, 4-day adult    12 digestive system, 20-day adult    11 fat body, larvae L3 wandering    7 fat body, white prepupae    13 fat body, pupae P8    13 carcass, larvae L3 wandering    11 carcass, 1-day adult    8 carcass, 4-day adult    8 carcass, 20-day adult    10 ovary, virgin 4-day female    29 ovary, mated 4-day female    24 testis, mated 4-day male    6 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    29 embryo 04-06hr    36 embryo 06-08hr    29 embryo 08-10hr    31 embryo 10-12hr    22 embryo 12-14hr    22 embryo 14-16hr    22 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    15 larva L2    13 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    19 white prepupae new    23 white prepupae 12hr    21 white prepupae 24hr    23 pupae 2d postWPP    19 pupae 3d postWPP    14 pupae 4d postWPP    8 adult male 01day    15 adult male 05day    16 adult male 30day    18 adult female 01day    17 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (58) embryo 02-04hr  (29) embryo 04-06hr  (36) embryo 06-08hr  (29) embryo 08-10hr  (31) embryo 10-12hr    22 embryo 12-14hr    22 embryo 14-16hr    22 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr  (29) embryo 22-24hr  (30) larva L1    15 larva L2    13 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    19 white prepupae new    23 white prepupae 12hr    21 white prepupae 24hr    23 pupae 2d postWPP    19 pupae 3d postWPP    14 pupae 4d postWPP    8 adult male 01day    15 adult male 05day    16 adult male 30day    18 adult female 01day    17 adult female 05day  26 adult female 30day  (29) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    29 embryo 04-06hr    36 embryo 06-08hr    29 embryo 08-10hr    31 embryo 10-12hr    22 embryo 12-14hr    22 embryo 14-16hr    22 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    15 larva L2    13 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    19 white prepupae new    23 white prepupae 12hr    21 white prepupae 24hr    23 pupae 2d postWPP    19 pupae 3d postWPP    14 pupae 4d postWPP    8 adult male 01day    15 adult male 05day    16 adult male 30day    18 adult female 01day    17 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    29 embryo 04-06hr    36 embryo 06-08hr    29 embryo 08-10hr    31 embryo 10-12hr    22 embryo 12-14hr    22 embryo 14-16hr    22 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    15 larva L2    13 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    19 white prepupae new    23 white prepupae 12hr    21 white prepupae 24hr    23 pupae 2d postWPP    19 pupae 3d postWPP    14 pupae 4d postWPP    8 adult male 01day    15 adult male 05day    16 adult male 30day    18 adult female 01day    17 adult female 05day    26 adult female 30day    29 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    29 embryo 04-06hr    36 embryo 06-08hr    29 embryo 08-10hr    31 embryo 10-12hr    22 embryo 12-14hr    22 embryo 14-16hr    22 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    15 larva L2    13 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    19 white prepupae new    23 white prepupae 12hr    21 white prepupae 24hr    23 pupae 2d postWPP    19 pupae 3d postWPP    14 pupae 4d postWPP    8 adult male 01day    15 adult male 05day    16 adult male 30day    18 adult female 01day    17 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (58) embryo 02-04hr  29 embryo 04-06hr  (36) embryo 06-08hr  29 embryo 08-10hr  31 embryo 10-12hr    22 embryo 12-14hr    22 embryo 14-16hr    22 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr  29 embryo 22-24hr  30 larva L1    15 larva L2    13 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    19 white prepupae new    23 white prepupae 12hr    21 white prepupae 24hr    23 pupae 2d postWPP    19 pupae 3d postWPP    14 pupae 4d postWPP    8 adult male 01day    15 adult male 05day    16 adult male 30day    18 adult female 01day    17 adult female 05day  26 adult female 30day  29 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    29 embryo 04-06hr    36 embryo 06-08hr    29 embryo 08-10hr    31 embryo 10-12hr    22 embryo 12-14hr    22 embryo 14-16hr    22 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    15 larva L2    13 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    19 white prepupae new    23 white prepupae 12hr    21 white prepupae 24hr    23 pupae 2d postWPP    19 pupae 3d postWPP    14 pupae 4d postWPP    8 adult male 01day    15 adult male 05day    16 adult male 30day    18 adult female 01day    17 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    29 embryo 04-06hr    36 embryo 06-08hr    29 embryo 08-10hr    31 embryo 10-12hr    22 embryo 12-14hr    22 embryo 14-16hr    22 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    15 larva L2    13 larva L3 12hr old    11 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    19 white prepupae new    23 white prepupae 12hr    21 white prepupae 24hr    23 pupae 2d postWPP    19 pupae 3d postWPP    14 pupae 4d postWPP    8 adult male 01day    15 adult male 05day    16 adult male 30day    18 adult female 01day    17 adult female 05day    26 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    31 embryonic 1182-4H    22 embryonic GM2    57 embryonic Kc167    48 embryonic S1    25 embryonic S3    87 leg disc CME L1    34 wing disc CME-W2    23 wing disc ML-DmD8    20 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    73 tumorous blood cells mbn2    56 ovary fGS/OSS    16 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (28) Schneider line 2 Sg4  (31) embryonic 1182-4H    22 embryonic GM2  (57) embryonic Kc167  (48) embryonic S1    25 embryonic S3  (87) leg disc CME L1  (34) wing disc CME-W2    23 wing disc ML-DmD8    20 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1  26 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2  (73) tumorous blood cells mbn2  (56) ovary fGS/OSS    16 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    31 embryonic 1182-4H    22 embryonic GM2    57 embryonic Kc167    48 embryonic S1    25 embryonic S3    87 leg disc CME L1    34 wing disc CME-W2    23 wing disc ML-DmD8    20 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    73 tumorous blood cells mbn2    56 ovary fGS/OSS    16 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    31 embryonic 1182-4H    22 embryonic GM2    57 embryonic Kc167    48 embryonic S1    25 embryonic S3    87 leg disc CME L1    34 wing disc CME-W2    23 wing disc ML-DmD8    20 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    73 tumorous blood cells mbn2    56 ovary fGS/OSS    16 ovary OSC    9 ovary OSS    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    31 embryonic 1182-4H    22 embryonic GM2    57 embryonic Kc167    48 embryonic S1    25 embryonic S3    87 leg disc CME L1    34 wing disc CME-W2    23 wing disc ML-DmD8    20 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    73 tumorous blood cells mbn2    56 ovary fGS/OSS    16 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  28 Schneider line 2 Sg4  31 embryonic 1182-4H    22 embryonic GM2  (57) embryonic Kc167  (48) embryonic S1    25 embryonic S3  (87) leg disc CME L1  (34) wing disc CME-W2    23 wing disc ML-DmD8    20 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1  26 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2  (73) tumorous blood cells mbn2  (56) ovary fGS/OSS    16 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    31 embryonic 1182-4H    22 embryonic GM2    57 embryonic Kc167    48 embryonic S1    25 embryonic S3    87 leg disc CME L1    34 wing disc CME-W2    23 wing disc ML-DmD8    20 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    73 tumorous blood cells mbn2    56 ovary fGS/OSS    16 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    31 embryonic 1182-4H    22 embryonic GM2    57 embryonic Kc167    48 embryonic S1    25 embryonic S3    87 leg disc CME L1    34 wing disc CME-W2    23 wing disc ML-DmD8    20 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    14 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    73 tumorous blood cells mbn2    56 ovary fGS/OSS    16 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult  (27) Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3  (27) Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult  (31) Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult  27 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3  27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult  31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Ire1GD3071 Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of Ire1 Class Mutagen Stocks Known lesion Ire1f02170 piggyBac transposase 2 -- Ire1e03681 1 -- Ire1NP5472 1 -- Ire1e02281 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ire1 Class Mutagen Stocks Known lesion Ire1GD3071 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Ire1GL00141 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ire1HMS03003 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{TRiP.GL00141} NA P{TRiP.HMS03003} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}Ire1e02281 NA PBac{RB}Ire1e03681 NA PBac{WH}Ire1f02170 NA insertion of enhancer trap binary system P{GawB}Ire1NP5472 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
endoribonuclease activity inferred from direct assay (Plongthongkum et al., 2007) inferred from mutant phenotype (Hollien and Weissman, 2006, Ryoo et al., 2007)
Biological Process
CV term Evidence References
endoplasmic reticulum unfolded protein response inferred from direct assay (Plongthongkum et al., 2007) inferred from mutant phenotype (Ryoo et al., 2007) Golgi organization inferred from mutant phenotype (Zacharogianni et al., 2011) HAC1-type intron splice site recognition and cleavage inferred from mutant phenotype (Ryoo et al., 2007) mRNA catabolic process inferred from mutant phenotype (Hollien and Weissman, 2006) regulation of RNA splicing inferred from direct assay (Plongthongkum et al., 2007) response to endoplasmic reticulum stress inferred from direct assay (Plongthongkum et al., 2007)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719 (FlyBase Curators et al., 2004-) endoribonuclease activity inferred from sequence or structural similarity with Saccharomyces IRE1 (Geething et al., 2000) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine kinase activity inferred from sequence or structural similarity with EMBL:AL035248 (FlyBase, 1992-) inferred from sequence or structural similarity with Saccharomyces IRE1 (Geething et al., 2000)
Biological Process
CV term Evidence References
protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000) response to unfolded protein inferred from sequence or structural similarity with Saccharomyces IRE1 (Geething et al., 2000)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from sequence or structural similarity with Saccharomyces IRE1 (Geething et al., 2000) membrane inferred from sequence or structural similarity with Saccharomyces IRE1 (Geething et al., 2000)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ire1 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261984 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42358 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • A8JR46
Orthologs
OrthoDB Orthologs (47) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6MSDHK Dipteran inclusive ortholog search EOG6CRM72 Insect inclusive ortholog search EOG6932CV Arthropod inclusive ortholog search EOG65DV4C Metazoa inclusive ortholog search EOG6JDW0M
Orthologs in Drosophila Species (EOG6MSDHK)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Ire1     Drosophila simulans   Dsim\GD20099 Y   Drosophila sechellia   Dsec\GM13269     Drosophila sechellia   Dsec\GM26893 Y   Drosophila erecta   Dere\GG15830 Y   Drosophila yakuba   Dyak\GE25112     Drosophila ananassae   Dana\GF17356 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18275 Y   Drosophila persimilis   Dper\GL11945 Y   Drosophila willistoni   Dwil\GK22812     Drosophila virilis   Dvir\GJ10185 Y   Drosophila mojavensis   Dmoj\GI10330 Y   Drosophila grimshawi   Dgri\GH23342 Y
Orthologs in non-Drosophila Dipterans (EOG6CRM72)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011308   Anopheles gambiae Malaria mosquito  Agam\AGAP004176   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001335
Orthologs in non-Dipteran Insects (EOG6932CV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\ire-1   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000789   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH24468   Atta cephalotes Leafcutter ant  Acep\ACEP15227   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15356   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14410   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL23648   Linepithema humile Argentine ant  Lhum\LH16200   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13906   Solenopsis invicta Red fire ant  Sinv\SINV16466   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005570   Bombyx mori Silkmoth  Bmor\BGIBMGA000180   Pediculus humanus Human body louse  Phum\PHUM343930   Tribolium castaneum Red flour beetle  Tcas\TC006276
Orthologs in non-Insect Arthropods (EOG65DV4C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_305906   Ixodes scapularis Deer tick  Isca\ISCW013137
Orthologs in non-Arthropod Metazoa (EOG6JDW0M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ire-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021235gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017184gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009434gn   Danio rerio Zebrafish  Drer\ern1   Danio rerio Zebrafish  Drer\ern2   Xenopus tropicalis Western clawed frog  Xtro\ern2   Gallus gallus Domestic chicken  Ggal\ERN1   Gallus gallus Domestic chicken  Ggal\ERN1   Mus musculus House mouse  Mmus\Ern2   Mus musculus House mouse  Mmus\Ern1   Rattus norvegicus Norway rat  Rnor\Ern1   Rattus norvegicus Norway rat  Rnor\Ern2   Homo sapiens Human  Hsap\ERN1   Homo sapiens Human  Hsap\ENSG00000134398
Human Orthologs (2)
Gene OMIM HGNC Hsap\ERN1     Hsap\ENSG00000134398
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20099 Drosophila sechellia Dsec\GM26893 Drosophila erecta Dere\GG15830 Drosophila ananassae Dana\GF17356 Drosophila pseudoobscura pseudoobscura Dpse\GA18275 Drosophila persimilis Dper\GL11945 Drosophila virilis Dvir\GJ10185 Drosophila mojavensis Dmoj\GI10330 Drosophila grimshawi Dgri\GH23342
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	35253 y1 sc* v1; P{TRiP.GL00141}attP2 36743 y1 sc* v1; P{TRiP.HMS03003}attP40 18520 w1118; PBac{WH}Ire1f02170/TM6B, Tb1
Harvard	- PBac{RB}Ire1e03681 - PBac{WH}Ire1f02170
Kyoto	105031 y* w*; P{GawB}Ire1NP5472 / TM6, P{UAS-lacZ.UW23-1}UW23-1
VDRC	v39562 w1118 P{GD3071}v39562 v39561 w1118; P{GD3071}v39561
Genomic Clones ( 2 )
	BACR02O09 BACR48D03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 14 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	IP02909
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	149243 193164 273719 CK01.9C.E5 Dmel_neb_003801_0685_0695 EC053542 EC056199 EK135708 EK174810 EK254422 EK296141 RP003516557 RP004429202
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261984 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42358
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ire-1 CG4583 (Vaughn et al., 2001) Source for identity of: Ire1 ire-1 (Hollien and Weissman, 2006)
Source for database merge of	Source for merge of: ire-1 Ire (FlyBase, 1992-)
Additional comments
Other Comments
	Changes in expression profiles associated with the unfolded protein response have been analyzed in S2 cells following treatment with dsRNA made from templates generated with primers directed against this gene. (Hollien and Weissman, 2006) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001104369 RefSeq (Proteins) • NP_001097839 DDBJ / EMBL / GenBank • ABW08704 ABW08705 CZ484678 Entrez Gene - A searchable database of RefSeq genes. • 42358 UniProtKB/TrEMBL • A8JR46 A8JR47
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Pyrrolo-quinoline quinone beta-propeller repeat (IPR018391) Quinonprotein alcohol dehydrogenase-like domain (IPR027295) Protein kinase, catalytic domain (IPR000719) PUG domain (IPR006567) Serine/threonine-protein kinase, active site (IPR008271) KEN domain (IPR010513) Protein kinase-like domain (IPR011009) Quinonprotein alcohol dehydrogenase-like superfamily (IPR011047)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261984 DRSC - Results from RNAi screens. • FBgn0261984 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261984 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42358 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42358 modMine - Data generated by the modENCODE project. • FBgn0261984 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • A8JR46
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	CG4583 (Fetherson and Vaughn, 2004, Pile et al., 2003, Brody et al., 2002, Vaughn et al., 2001, Hollien and Weissman, 2006, Ling and Salvaterra, 2011, Ni et al., 2011.7.19, Qin et al., 2008) dire-1 (Vaughn et al., 2001) dIRE1 (Geething et al., 2000) Ire   ire1 (Kang et al., 2012) ire-1 (Zacharogianni et al., 2011, Mattila et al., 2008, Ryoo et al., 2007, Kang and Ryoo, 2008, Kang and Ryoo, 2007, ) IRE1 (Hollien and Weissman, 2006, Hollien and Weissman, 2006, Qin et al., 2008) Ire1 (Plongthongkum et al., 2007, ) Ire-1 (Ryoo and Steller, 2007, Ling and Salvaterra, 2011) IRE-like (Morrison et al., 2000)
Name Synonym	Inositol required 1 (Plongthongkum et al., 2007) Inositol-requiring enzyme-1 (Hollien and Weissman, 2006, Ling and Salvaterra, 2011) inositol-requiring enzyme-1 (Qin et al., 2008) Ire   ire-1
Secondary FlyBase IDs
	FBgn0038736 FBgn0041204
References ( 34 )
Generate a list of	All references relating to this gene ( 34 )
List References by type	Research paper  ( 10 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 5 ) FlyBase analysis  ( 2 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Kang et al., 2012, Nat. Cell Biol. 14(4): 409--415 CDK5 and MEKK1 mediate pro-apoptotic signalling following endoplasmic reticulum stress in an autosomal dominant retinitis pigmentosa model. [FBrf0217924] Ling and Salvaterra, 2011, PLoS ONE 6(3): e17762 Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis. [FBrf0213272] Zacharogianni et al., 2011, EMBO J. 30(18): 3684--3700 ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association. [FBrf0215221] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...