Gene Dmel\Ptpmeg

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

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Protein-tyrosine phosphatase, receptor/non-receptor type (IPR000242)

FERM domain (IPR000299)

Protein-tyrosine/Dual-specificity phosphatase (IPR000387)

PDZ/DHR/GLGF (IPR001478)

Pleckstrin homology-type (IPR011993)

Protein-tyrosine phosphatase, non-receptor type-3, -4 (IPR012151)

FERM adjacent (FA) (IPR014847)

FERM/acyl-CoA-binding protein, 3-helical bundle (IPR014352)

Protein-tyrosine phosphatase, active site (IPR016130)

FERM, C-terminal PH-like domain (IPR018980)

FERM conserved site (IPR019747)

FERM central domain (IPR019748)

Band 4.1 family (IPR019750)

FERM, N-terminal (IPR018979)

Band 4.1 domain (IPR019749)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

(The modENCODE Consortium, 2010)

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

High-Throughput Expression Data

Untitled Document detailed view

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

mE2_34_mRNA_expression_cluster_30

External Data & Images

neuroanatomy defective | recessive

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

viable, with Scer\GAL4^pnr-MD237

Ptpmeg^GD7557

Sterility

Allele

fertile

Other Phenotypes

Allele

behavior defective

Ptpmeg¹

neuroanatomy defective (with Df(3L)ED201)

Ptpmeg¹

visible, with Scer\GAL4^GMR.PF

Ptpmeg^GS9137

Phenotype manifest in

Allele

axon & ellipsoid body

Ptpmeg¹

axon & ellipsoid body (with Df(3L)ED201)

Ptpmeg¹

axon & mushroom body | somatic clone | cell non-autonomous

Ptpmeg¹

eye, with Scer\GAL4^GMR.PF

Ptpmeg^GS9137

mushroom body alpha-lobe

Ptpmeg¹

mushroom body alpha-lobe (with Df(3L)ED201)

mushroom body beta-lobe

Ptpmeg¹

mushroom body medial lobe

Ptpmeg¹

mushroom body vertical lobe

Ptpmeg¹

Classical Alleles ( 33 )

For All Classical Alleles Show

Allele of Ptpmeg	Mutagen	Stocks	Known lesion
Ptpmeg^f01047	piggyBac transposase	2	--
Ptpmeg^NP4498		2	--
Ptpmeg⁰³		1	Yes
Ptpmeg¹	recombination SCEI endonuclease FLPase	1	Yes
Ptpmeg^5-SZ-3091		1	--
Ptpmeg^c06290	piggyBac transposase	1	--
Ptpmeg^c06293	piggyBac transposase	1	--
Ptpmeg^c06880	piggyBac transposase	1	--
Ptpmeg^d03310	P-element activity	1	--
Ptpmeg^d05374	P-element activity	1	--
Ptpmeg^DG23409	P-element activity	1	--
Ptpmeg^e03119	piggyBac transposase	1	--
Ptpmeg^e04126	piggyBac transposase	1	--
Ptpmeg^EP3542		1	--
Ptpmeg^EY12179	P-element activity	1	--
Ptpmeg^EY13883	P-element activity	1	--
Ptpmeg^EY14306	P-element activity	1	--
Ptpmeg^EY23549	P-element activity	1	--
Ptpmeg^f04972	piggyBac transposase	1	--
Ptpmeg^f06198	piggyBac transposase	1	--
Ptpmeg^G8954	P-element activity	1	--
Ptpmeg^GS3223		1	--
Ptpmeg^GS9137		1	Yes
Ptpmeg^MB04912		1	--
Ptpmeg^MI00492		1	--
Ptpmeg^c02341	piggyBac transposase	0	--
Ptpmeg^c02785	piggyBac transposase	0	--
Ptpmeg^c05544	piggyBac transposase	0	--
Ptpmeg^c05591	piggyBac transposase	0	--
Ptpmeg^CPTI100033	piggyBac transposase	0	Yes
Ptpmeg^EY10853	P-element activity	0	--
Ptpmeg^f05405	piggyBac transposase	0	--
Ptpmeg^f06210	piggyBac transposase	0	--

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Ptpmeg	Mutagen	Stocks	Known lesion
Ptpmeg^GD7557	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Ptpmeg^KK102294	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Ptpmeg^{C877S.Scer\UAS}	in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement	0	Yes
Ptpmeg^{G494A.F494A.Scer\UAS}	in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement	0	Yes
Ptpmeg^{R883M.Scer\UAS}	in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement	0	Yes
Ptpmeg^{Scer\SceI.RS.cWa}	in vitro construct	0	Yes
Ptpmeg^Scer\UAS.cWa	in vitro construct - regulatory fusion	0	Yes
Ptpmeg^{Y650F.D787A.Scer\UAS}	in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement	0	Yes
Ptpmeg^{ΔFERM.Scer\UAS}	in vitro construct - coding region fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7557}

P{KK102294}

P{UAS-Ptpmeg.C877S}

P{UAS-Ptpmeg.G494A.F494A}

P{UAS-Ptpmeg.R883M}

P{UAS-Ptpmeg.W}

P{UAS-Ptpmeg.Y650F.D787A}

P{UAS-Ptpmeg.ΔFERM}

characterization construct

P{FRT(w^hs.Ptpmeg.Scer\SceI.RS)}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Ptpmeg^EP3542

P{EP}Ptpmeg^G8954

P{EPgy2}Ptpmeg^EY10853

P{EPgy2}Ptpmeg^EY12179

P{EPgy2}Ptpmeg^EY13883

P{EPgy2}Ptpmeg^EY14306

P{EPgy2}Ptpmeg^EY23549

miscellaneous insertions

Mi{MIC}Ptpmeg^MI00492

P{RS5}Ptpmeg^5-SZ-3091

P{UAST-YFP.Rab40}Ptpmeg⁰³

P{wHy}Ptpmeg^DG23409

PBac{544.SVS-1}Ptpmeg^CPTI100033

PBac{PB}Ptpmeg^c02341

PBac{PB}Ptpmeg^c02785

PBac{PB}Ptpmeg^c05544

PBac{PB}Ptpmeg^c05591

PBac{PB}Ptpmeg^c06290

PBac{PB}Ptpmeg^c06293

PBac{PB}Ptpmeg^c06880

PBac{RB}Ptpmeg^e03119

PBac{RB}Ptpmeg^e04126

PBac{WH}Ptpmeg^f01047

PBac{WH}Ptpmeg^f04972

PBac{WH}Ptpmeg^f05405

PBac{WH}Ptpmeg^f06198

PBac{WH}Ptpmeg^f06210

insertion of enhancer trap binary system

Mi{ET1}mth^MB04912

P{GawB}Ptpmeg^NP4498

Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )

Terms Based on Experimental Evidence ( 5 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

axon extension involved in axon guidance

References

autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

inferred from mutant phenotype

central complex development

inferred from mutant phenotype

mushroom body development

inferred from mutant phenotype

salivary gland cell autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 4 terms )

Molecular Function

CV term

cytoskeletal protein binding

References

inferred from electronic annotation with InterPro:IPR012151

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

protein tyrosine phosphatase activity

inferred from sequence or structural similarity

(Edwards et al., 2001)

inferred from sequence or structural similarity with UniProtKB:P29074

(Edwards, 1999.12.16)

non-traceable author statement

(Morrison et al., 2000)

Biological Process

CV term

protein dephosphorylation

References

inferred from electronic annotation with InterPro:IPR000242, InterPro:IPR012151

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Edwards et al., 2001)

non-traceable author statement

(Morrison et al., 2000)

Cellular Component

CV term

(FlyBase Curators et al., 2004-)

References

cytoskeleton

inferred from electronic annotation with InterPro:IPR000299

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Protein-protein

Interacting group

Assay

References

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Ptpmeg allele

Gene

References

unspecified

pbl

(Gregory et al., 2007)

External Data

DroID - A comprehensive database of gene and protein interactions.

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FBgn0261985

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

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38059

Orthologs

Genome-wide drosophilid orthologs

Dana\GF24903

Dere\GG14719

Dgri\GH15457

Dmoj\GI16737

Dper\GL16093

Dpse\GA11530

Dsec\GM14335

Dsim\GD13561

Dvir\GJ12484

Dwil\GK13061

Dyak\GE21082

Curated drosophilid orthologs

Dpse\GA11530

Stocks & Reagents

Stocks Listed in FlyBase ( 30 )

Harvard

P{XP}Ptpmeg^d03310

P{XP}Ptpmeg^d05374

PBac{PB}Ptpmeg^c06293

PBac{PB}Ptpmeg^c06880

PBac{RB}Ptpmeg^e03119

PBac{RB}Ptpmeg^e04126

PBac{WH}Ptpmeg^f01047

PBac{WH}Ptpmeg^f04972

PBac{WH}Ptpmeg^f06198

VDRC

v103740

P{KK102294}VIE-260B

v38651

w¹¹¹⁸; P{GD7557}v38651

v38652

w¹¹¹⁸; P{GD7557}v38652

More stocks available...

Genomic Clones ( 1 )

BACR13F06

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 137 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

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FBgn0261985

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

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38059

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Ptpmeg CG1228

Source for database merge of

Source for merge of: Ptpmeg anon-WO0118547.211

Source for merge of: Ptpmeg Meg

(Edwards et al., 2001)

Additional comments

Source for merge of Ptpmeg anon-WO0118547.211 was sequence comparison (date:051113).

Source for identity of Ptpmeg CG1228 was sequence comparison (date:000327).

Other Comments

FlyBase curator comment: although GenBank/EMBL/DDBJ: AF323978 refers to Ptpmeg as "split central complex", the ability of a scc allele to complement Ptpmeg phenotypes and to produce full-length Ptpmeg protein suggests that these genes are separate.

The Ptpmeg product is required for the proper establishment of axon projections in the ellipsoid body and is required for stabilization of already-formed projection patterns in the mushroom body. The phosphatase activity of the protein is needed for these functions.