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Gene Dmel\RASSF8

General Information
Symbol	Dmel\RASSF8	Species	D. melanogaster
Name		Annotation symbol	CG5053
Feature type	protein_coding_gene	FlyBase ID	FBgn0261986
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	96E8-96E8	Sequence location	3R:21,487,517..21,491,362 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\RASSF8 (CG5053, FBgn0261986). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: dsRNA transport; compound eye development; negative regulation of multicellular organism growth. 9 alleles are reported. The phenotypes of these alleles are annotated with: eye; interommatidial bristle; inter-ommatidial cell; wing; ommatidium. It has one annotated transcript and one annotated polypeptide. Protein features are: Ras-association. Gene sequence location is 3R:21487517..21491362.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96E8-96E8   Limits computationally determined from genome sequence between P{EP}EP3354 and P{PZ}l(3)rQ197rQ197
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RASSF8 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RASSF8-RA FBtr0084898  NM_143154   3429   607
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RASSF8-PA   FBpp0084272   69.1   607   6.52     NP_651411   AAF56488
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ras-association (IPR000159)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201568   AA440757   AA539855   AA736175   AA816262   AE014297 AAF56488   AI062071   AI063267   AI108061   AI237937   AI534364   AW940024   AY047500 AAK77232   BF489414   BF489985   BI168849   BI235285   BI236918   BI364178   BI365762   BI483936   BI486105   BI487187   BI578856   BI580069   BI580892   CO269885   CO272051   CO273154   CO293507   CO295146   CO302900   CO304592   CO311209   CO322776   CO329236   CO329564   EC064775   EC065713   EC070984   EC070985   EC072277   EC074307   EC090231   EC195958   EC197363   EC197841   EC209311   EC223547   EC224663   EC227740   EC234920   EC247167   GH751960   GH752129   GH753480   GH755071   GH755149   GH759058   GH759957   GH772042   GH777451   GH780468   GH784842   GH785612   GH786496   GH793522   GH804267   GH806069   GH806851   GH807287   GH810220   GH818800   GH819675   GH824334   GH825462   GH826510   GH829064   GH830924   GH834817   GH840516   GH844369   GH851582   GH853719   GH854981   GH856728   GH860019   GH864159   GH864918   GH865521   GH871525   GH873502   GH873973   GH878383   GH881264   GH882538   GH883068   GH884350   GH889563   GH896318   GH899075   GH901846   GH902171   GH904667   GH904790   GH906060   GH907126   GH908979   GH913155   GH914214   GH921478   GH927304   GH932137
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VBP2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
adherens junction inferred from direct assay (Langton et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    21 central nervous system, pupae P8    44 head, virgin 1-day female    25 head, virgin 4-day female    25 head, virgin 20-day female    13 head, mated 1-day female    25 head, mated 4-day female    17 head, mated 20-day female    13 head, mated 1-day male    37 head, mated 4-day male    30 head, mated 20-day male    30 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    72 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    7 fat body, larvae L3 wandering    6 fat body, white prepupae    12 fat body, pupae P8    19 carcass, larvae L3 wandering    25 carcass, 1-day adult    11 carcass, 4-day adult    13 carcass, 20-day adult    11 ovary, virgin 4-day female    83 ovary, mated 4-day female    83 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (30) central nervous system, larvae L3    21 central nervous system, pupae P8  (44) head, virgin 1-day female    25 head, virgin 4-day female    25 head, virgin 20-day female    13 head, mated 1-day female    25 head, mated 4-day female    17 head, mated 20-day female    13 head, mated 1-day male  (37) head, mated 4-day male  (30) head, mated 20-day male  (30) salivary gland, larvae L3 wandering  (32) salivary gland, white prepupae  (72) digestive system, larvae L3 wandering    12 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    7 fat body, larvae L3 wandering    6 fat body, white prepupae    12 fat body, pupae P8    19 carcass, larvae L3 wandering    25 carcass, 1-day adult    11 carcass, 4-day adult    13 carcass, 20-day adult    11 ovary, virgin 4-day female  (83) ovary, mated 4-day female  (83) testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    21 central nervous system, pupae P8    44 head, virgin 1-day female    25 head, virgin 4-day female    25 head, virgin 20-day female    13 head, mated 1-day female    25 head, mated 4-day female    17 head, mated 20-day female    13 head, mated 1-day male    37 head, mated 4-day male    30 head, mated 20-day male    30 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    72 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    7 fat body, larvae L3 wandering    6 fat body, white prepupae    12 fat body, pupae P8    19 carcass, larvae L3 wandering    25 carcass, 1-day adult    11 carcass, 4-day adult    13 carcass, 20-day adult    11 ovary, virgin 4-day female    83 ovary, mated 4-day female    83 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    21 central nervous system, pupae P8    44 head, virgin 1-day female    25 head, virgin 4-day female    25 head, virgin 20-day female    13 head, mated 1-day female    25 head, mated 4-day female    17 head, mated 20-day female    13 head, mated 1-day male    37 head, mated 4-day male    30 head, mated 20-day male    30 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    72 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    7 fat body, larvae L3 wandering    6 fat body, white prepupae    12 fat body, pupae P8    19 carcass, larvae L3 wandering    25 carcass, 1-day adult    11 carcass, 4-day adult    13 carcass, 20-day adult    11 ovary, virgin 4-day female    83 ovary, mated 4-day female    83 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    21 central nervous system, pupae P8    44 head, virgin 1-day female    25 head, virgin 4-day female    25 head, virgin 20-day female    13 head, mated 1-day female    25 head, mated 4-day female    17 head, mated 20-day female    13 head, mated 1-day male    37 head, mated 4-day male    30 head, mated 20-day male    30 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    72 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    7 fat body, larvae L3 wandering    6 fat body, white prepupae    12 fat body, pupae P8    19 carcass, larvae L3 wandering    25 carcass, 1-day adult    11 carcass, 4-day adult    13 carcass, 20-day adult    11 ovary, virgin 4-day female    83 ovary, mated 4-day female    83 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  30 central nervous system, larvae L3    21 central nervous system, pupae P8  (44) head, virgin 1-day female    25 head, virgin 4-day female    25 head, virgin 20-day female    13 head, mated 1-day female    25 head, mated 4-day female    17 head, mated 20-day female    13 head, mated 1-day male  (37) head, mated 4-day male  30 head, mated 20-day male  30 salivary gland, larvae L3 wandering  32 salivary gland, white prepupae  (72) digestive system, larvae L3 wandering    12 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    7 fat body, larvae L3 wandering    6 fat body, white prepupae    12 fat body, pupae P8    19 carcass, larvae L3 wandering    25 carcass, 1-day adult    11 carcass, 4-day adult    13 carcass, 20-day adult    11 ovary, virgin 4-day female  (83) ovary, mated 4-day female  (83) testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    21 central nervous system, pupae P8    44 head, virgin 1-day female    25 head, virgin 4-day female    25 head, virgin 20-day female    13 head, mated 1-day female    25 head, mated 4-day female    17 head, mated 20-day female    13 head, mated 1-day male    37 head, mated 4-day male    30 head, mated 20-day male    30 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    72 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    7 fat body, larvae L3 wandering    6 fat body, white prepupae    12 fat body, pupae P8    19 carcass, larvae L3 wandering    25 carcass, 1-day adult    11 carcass, 4-day adult    13 carcass, 20-day adult    11 ovary, virgin 4-day female    83 ovary, mated 4-day female    83 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    30 central nervous system, larvae L3    21 central nervous system, pupae P8    44 head, virgin 1-day female    25 head, virgin 4-day female    25 head, virgin 20-day female    13 head, mated 1-day female    25 head, mated 4-day female    17 head, mated 20-day female    13 head, mated 1-day male    37 head, mated 4-day male    30 head, mated 20-day male    30 salivary gland, larvae L3 wandering    32 salivary gland, white prepupae    72 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    6 digestive system, 4-day adult    11 digestive system, 20-day adult    7 fat body, larvae L3 wandering    6 fat body, white prepupae    12 fat body, pupae P8    19 carcass, larvae L3 wandering    25 carcass, 1-day adult    11 carcass, 4-day adult    13 carcass, 20-day adult    11 ovary, virgin 4-day female    83 ovary, mated 4-day female    83 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    38 embryo 02-04hr    39 embryo 04-06hr    15 embryo 06-08hr    18 embryo 08-10hr    26 embryo 10-12hr    20 embryo 12-14hr    21 embryo 14-16hr    13 embryo 16-18hr    15 embryo 18-20hr    9 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    5 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    13 white prepupae 24hr    16 pupae 2d postWPP    12 pupae 3d postWPP    10 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    7 adult female 01day    11 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (38) embryo 02-04hr  (39) embryo 04-06hr    15 embryo 06-08hr    18 embryo 08-10hr  26 embryo 10-12hr    20 embryo 12-14hr    21 embryo 14-16hr    13 embryo 16-18hr    15 embryo 18-20hr    9 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    5 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    13 white prepupae 24hr    16 pupae 2d postWPP    12 pupae 3d postWPP    10 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    7 adult female 01day    11 adult female 05day  (27) adult female 30day  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    38 embryo 02-04hr    39 embryo 04-06hr    15 embryo 06-08hr    18 embryo 08-10hr    26 embryo 10-12hr    20 embryo 12-14hr    21 embryo 14-16hr    13 embryo 16-18hr    15 embryo 18-20hr    9 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    5 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    13 white prepupae 24hr    16 pupae 2d postWPP    12 pupae 3d postWPP    10 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    7 adult female 01day    11 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    38 embryo 02-04hr    39 embryo 04-06hr    15 embryo 06-08hr    18 embryo 08-10hr    26 embryo 10-12hr    20 embryo 12-14hr    21 embryo 14-16hr    13 embryo 16-18hr    15 embryo 18-20hr    9 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    5 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    13 white prepupae 24hr    16 pupae 2d postWPP    12 pupae 3d postWPP    10 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    7 adult female 01day    11 adult female 05day    27 adult female 30day    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    38 embryo 02-04hr    39 embryo 04-06hr    15 embryo 06-08hr    18 embryo 08-10hr    26 embryo 10-12hr    20 embryo 12-14hr    21 embryo 14-16hr    13 embryo 16-18hr    15 embryo 18-20hr    9 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    5 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    13 white prepupae 24hr    16 pupae 2d postWPP    12 pupae 3d postWPP    10 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    7 adult female 01day    11 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (38) embryo 02-04hr  (39) embryo 04-06hr    15 embryo 06-08hr    18 embryo 08-10hr  26 embryo 10-12hr    20 embryo 12-14hr    21 embryo 14-16hr    13 embryo 16-18hr    15 embryo 18-20hr    9 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    5 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    13 white prepupae 24hr    16 pupae 2d postWPP    12 pupae 3d postWPP    10 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    7 adult female 01day    11 adult female 05day  27 adult female 30day  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    38 embryo 02-04hr    39 embryo 04-06hr    15 embryo 06-08hr    18 embryo 08-10hr    26 embryo 10-12hr    20 embryo 12-14hr    21 embryo 14-16hr    13 embryo 16-18hr    15 embryo 18-20hr    9 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    5 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    13 white prepupae 24hr    16 pupae 2d postWPP    12 pupae 3d postWPP    10 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    7 adult female 01day    11 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    38 embryo 02-04hr    39 embryo 04-06hr    15 embryo 06-08hr    18 embryo 08-10hr    26 embryo 10-12hr    20 embryo 12-14hr    21 embryo 14-16hr    13 embryo 16-18hr    15 embryo 18-20hr    9 embryo 20-22hr    21 embryo 22-24hr    21 larva L1    5 larva L2    3 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    13 white prepupae new    14 white prepupae 12hr    13 white prepupae 24hr    16 pupae 2d postWPP    12 pupae 3d postWPP    10 pupae 4d postWPP    5 adult male 01day    7 adult male 05day    8 adult male 30day    7 adult female 01day    11 adult female 05day    27 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    20 embryonic 1182-4H    19 embryonic GM2    23 embryonic Kc167    16 embryonic S1    15 embryonic S3    15 leg disc CME L1    25 wing disc CME-W2    18 wing disc ML-DmD8    27 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    16 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    30 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    25 ovary fGS/OSS    22 ovary OSC    22 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (27) Schneider line 2 Sg4    20 embryonic 1182-4H    19 embryonic GM2    23 embryonic Kc167    16 embryonic S1    15 embryonic S3    15 leg disc CME L1    25 wing disc CME-W2    18 wing disc ML-DmD8  (27) wing disc ML-DmD9    17 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    16 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1  (30) CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    25 ovary fGS/OSS    22 ovary OSC    22 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    20 embryonic 1182-4H    19 embryonic GM2    23 embryonic Kc167    16 embryonic S1    15 embryonic S3    15 leg disc CME L1    25 wing disc CME-W2    18 wing disc ML-DmD8    27 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    16 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    30 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    25 ovary fGS/OSS    22 ovary OSC    22 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    20 embryonic 1182-4H    19 embryonic GM2    23 embryonic Kc167    16 embryonic S1    15 embryonic S3    15 leg disc CME L1    25 wing disc CME-W2    18 wing disc ML-DmD8    27 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    16 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    30 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    25 ovary fGS/OSS    22 ovary OSC    22 ovary OSS    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    20 embryonic 1182-4H    19 embryonic GM2    23 embryonic Kc167    16 embryonic S1    15 embryonic S3    15 leg disc CME L1    25 wing disc CME-W2    18 wing disc ML-DmD8    27 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    16 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    30 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    25 ovary fGS/OSS    22 ovary OSC    22 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  27 Schneider line 2 Sg4    20 embryonic 1182-4H    19 embryonic GM2    23 embryonic Kc167    16 embryonic S1    15 embryonic S3    15 leg disc CME L1    25 wing disc CME-W2    18 wing disc ML-DmD8  27 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    16 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1  30 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    25 ovary fGS/OSS    22 ovary OSC    22 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    20 embryonic 1182-4H    19 embryonic GM2    23 embryonic Kc167    16 embryonic S1    15 embryonic S3    15 leg disc CME L1    25 wing disc CME-W2    18 wing disc ML-DmD8    27 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    16 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    30 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    25 ovary fGS/OSS    22 ovary OSC    22 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    20 embryonic 1182-4H    19 embryonic GM2    23 embryonic Kc167    16 embryonic S1    15 embryonic S3    15 leg disc CME L1    25 wing disc CME-W2    18 wing disc ML-DmD8    27 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    16 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    30 CNS ML-DmBG1-c1    23 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    25 ovary fGS/OSS    22 ovary OSC    22 ovary OSS    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    63 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    53 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult  (27) Cadmium 100 mM 48 hrs, 4-day adult  (63) Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult  (53) Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (38) Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (60) Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (49) Paraquat 10 mM 48 hrs, 4-day adult  (30) Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    63 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    53 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    63 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    53 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    63 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    53 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult  27 Cadmium 100 mM 48 hrs, 4-day adult  (63) Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult  (53) Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (38) Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (60) Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (49) Paraquat 10 mM 48 hrs, 4-day adult  30 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    63 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    53 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    63 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    53 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    38 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    60 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    8 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • GH01133
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 RASSF8GD11517 Other Phenotypes Allele decreased cell death RASSF86 decreased cell death | somatic clone RASSF86 increased cell number RASSF86 visible RASSF86 Phenotype manifest in Allele eye RASSF86 interommatidial bristle RASSF86 inter-ommatidial cell RASSF86 ommatidium RASSF86 wing RASSF86
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of RASSF8 Class Mutagen Stocks Known lesion RASSF8G15974 P-element activity 1 -- RASSF82 P-element activity 0 Yes RASSF84 P-element activity 0 Yes RASSF86 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RASSF8 Class Mutagen Stocks Known lesion RASSF8GD11517 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RASSF8JF02955 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RASSF8KK100500 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RASSF8NIG.5053R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes RASSF8Scer\UAS.P\T.cLa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3R)RASSF86 (Langton et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.5053R} NA P{UASp-RASSF8.L} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}GE15974 NA P{EP}RASSF84 NA P{EP}RASSF8G15974 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
compound eye development inferred from mutant phenotype (Langton et al., 2009) dsRNA transport inferred from mutant phenotype (Saleh et al., 2006) negative regulation of multicellular organism growth inferred from mutant phenotype (Langton et al., 2009)
Cellular Component
CV term Evidence References
adherens junction inferred from direct assay (Langton et al., 2009)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
signal transduction inferred from electronic annotation with InterPro:IPR000159 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene (Langton et al., 2007) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
RASSF8 - ASPP anti tag coimmunoprecipitation, anti tag western blot (Langton et al., 2009) RASSF8 - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - CG12003 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - CG5726 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - CG6724 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - CG8545 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - LKR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - Plod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - Prosβ5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - RpS11 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - Rpb5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - eEF1δ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - ppan anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RASSF8 - sls anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RASSF8 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261986 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43096
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6K3MFX Dipteran inclusive ortholog search EOG6TTGS6 Insect inclusive ortholog search EOG6JDG3G Arthropod inclusive ortholog search EOG66HDSB Metazoa inclusive ortholog search EOG6GJBH0
Orthologs in Drosophila Species (EOG6K3MFX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\RASSF8     Drosophila simulans   Dsim\GD21229 Y   Drosophila sechellia   Dsec\GM10258 Y   Drosophila erecta   Dere\GG11418 Y   Drosophila yakuba   Dyak\GE23613 Y   Drosophila ananassae   Dana\GF16765 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18623 Y   Drosophila persimilis   Dper\GL21526 Y   Drosophila willistoni   Dwil\GK12853 Y   Drosophila virilis   Dvir\GJ10971 Y   Drosophila mojavensis   Dmoj\GI22412 Y   Drosophila grimshawi   Dgri\GH18985 Y
Orthologs in non-Drosophila Dipterans (EOG6TTGS6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014487   Anopheles gambiae Malaria mosquito  Agam\AGAP001370   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ016617
Orthologs in non-Dipteran Insects (EOG6JDG3G)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB52726   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012942   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19695   Atta cephalotes Leafcutter ant  Acep\ACEP13718   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18310   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21766   Linepithema humile Argentine ant  Lhum\LH11015   Pogonomyrmex barbatus Red harvester ant  Pbar\PB23893   Solenopsis invicta Red fire ant  Sinv\SINV21636   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002426   Bombyx mori Silkmoth  Bmor\BGIBMGA010157   Pediculus humanus Human body louse  Phum\PHUM297970   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07768
Orthologs in non-Insect Arthropods (EOG66HDSB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW017591
Orthologs in non-Arthropod Metazoa (EOG6GJBH0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020529gn   Danio rerio Zebrafish  Drer\rassf8b   Danio rerio Zebrafish  Drer\si:ch211-261n11.3   Danio rerio Zebrafish  Drer\CABZ01003826.1   Danio rerio Zebrafish  Drer\rassf7b   Danio rerio Zebrafish  Drer\ENSDARG00000094325   Xenopus tropicalis Western clawed frog  Xtro\rassf8   Xenopus tropicalis Western clawed frog  Xtro\rassf7   Gallus gallus Domestic chicken  Ggal\RASSF8   Gallus gallus Domestic chicken  Ggal\RASSF7   Mus musculus House mouse  Mmus\Rassf8   Mus musculus House mouse  Mmus\Rassf7   Rattus norvegicus Norway rat  Rnor\Rassf7   Rattus norvegicus Norway rat  Rnor\Rassf8   Homo sapiens Human  Hsap\ENSG00000099849   Homo sapiens Human  Hsap\ENSG00000123094
Human Orthologs (2)
Gene OMIM HGNC Hsap\ENSG00000099849     Hsap\ENSG00000123094
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21229 Drosophila sechellia Dsec\GM10258 Drosophila erecta Dere\GG11418 Drosophila yakuba Dyak\GE23613 Drosophila ananassae Dana\GF16765 Drosophila pseudoobscura pseudoobscura Dpse\GA18623 Drosophila persimilis Dper\GL21526 Drosophila willistoni Dwil\GK12853 Drosophila virilis Dvir\GJ10971 Drosophila mojavensis Dmoj\GI22412 Drosophila grimshawi Dgri\GH18985
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	31807 w1118; P{EP}RASSF8G15974 28323 y1 v1; P{TRiP.JF02955}attP2
VDRC	v105823 P{KK100500}VIE-260B v42019 w1118; P{GD11517}v42019
Genomic Clones ( 2 )
	BACR09D08 BACR48J06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 110 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH01133
Other clones	DPiM_GH01133
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT25531 AT26243 GH06784 GH13111 HL08116 LD01094 LD04640 LD15630 LD19134 LD33702 RE09742 RE31111 RE33048 RE49241 RE51141 RE66906 RE69479 RE70706 RE72787 RE74201 RE75139 SD07164
Other clones	7758 10206 28390 59426 61443 62938 73790 79084 101027 122186 190111 190950 CK01.79A.B10 CK01.110D.G1 CK01.129D.H9 EK096229 EK105725 EK109835 EK185122 EK188846 EK203148 EK208742 EK223744 EK245231 EK292326 EK293120 RP003001268 RP003012747 RP003024603 RP003034825 RP003041020 RP003046316 RP003051672 RP003058663 RP003061668 RP003088284 RP003090149 RP003092615 RP003092656 RP003112370 RP003125387 RP003129961 RP003133450 RP003133840 RP003139322 RP003142527 RP003170618 RP003191134 RP003195015 RP003200797 RP003207815 RP003208107 RP003210026 RP003211343 RP003216927 RP003217080 RP003219679 RP003271411 RP003276678 RP003280242 RP003299200 RP003306540 RP003306708 RP003312608 RP003314067 RP003314839 RP003337170 RP003343178 RP003377157 RP003377826 RP003395615 RP003407683 RP003416833 RP003426860 RP003442275 RP003445068 RP003459923 RP003465964 RP003483207 RP003486506 RP003533800 RP003538216 RP003559092 RP003588507 RP003654766 RP003658269
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261986 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43096
Antibody Information
	polyclonal (Langton et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: RASSF8 CG5053 (Langton et al., 2009)
Source for database merge of	Source for merge of: RASSF8 Boa (FlyBase, 1992-)
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143154 RefSeq (Proteins) • NP_651411 DDBJ / EMBL / GenBank • AAF56488 AAK77232 AI062071 AW940024 AY047500 Entrez Gene - A searchable database of RefSeq genes. • 43096 UniProtKB/TrEMBL • Q9VBP2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ras-association (IPR000159) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH01133
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261986 DRSC - Results from RNAi screens. • FBgn0261986 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261986 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43096 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43096 modMine - Data generated by the modENCODE project. • FBgn0261986
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	Boa (Langton et al., 2007) CG5053 (Sherwood et al., 2008, Langton et al., 2009, Saleh et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009, Brandt, 2006, Pavlidis et al., 2012, Japanese National Institute of Genetics, 2012.5.21) dmRASSF7/8 (Sherwood et al., 2008) dRASSF8 (Langton et al., 2009) RASSF8
Name Synonym
Secondary FlyBase IDs
	FBgn0039352 FBgn0086725
References ( 21 )
Generate a list of	All references relating to this gene ( 21 )
List References by type	Research paper  ( 4 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Pavlidis et al., 2012, Mol. Biol. Evol. 29(10): 3237--3248 A critical assessment of storytelling: gene ontology categories and the importance of validating genomic scans. [FBrf0219628] Show all research papers ...