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Gene Dmel\RASSF8

General Information
Symbol	Dmel\RASSF8	Species	D. melanogaster
Name		Annotation symbol	CG5053
Feature type	protein_coding_gene	FlyBase ID	FBgn0261986
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	96E8-96E8	Sequence location	3R:21,487,522..21,491,366 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\RASSF8 (CG5053, FBgn0261986). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: dsRNA transport; compound eye development; negative regulation of multicellular organism growth. 8 alleles are reported. The phenotypes of these alleles are annotated with: eye; interommatidial bristle; inter-ommatidial cell; wing; ommatidium. It has one annotated transcript and one annotated polypeptide. Protein features are: Ras-association. Gene sequence location is 3R:21487522..21491366.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN20596 HFA15707
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96E8-96E8   Limits computationally determined from genome sequence between P{EP}EP3354 and P{PZ}l(3)rQ197rQ197
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RASSF8 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RASSF8-RA FBtr0084898  NM_143154   3428   607
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RASSF8-PA   FBpp0084272   69.1   607   6.52     NP_651411   AAF56488
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ras-association (IPR000159)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201568   AA440757   AA539855   AA736175   AA816262   AE014297 AAF56488   AI062071   AI063267   AI108061   AI237937   AI534364   AW940024   AY047500 AAK77232   BF489414   BF489985   BI168849   BI235285   BI236918   BI364178   BI365762   BI483936   BI486105   BI487187   BI578856   BI580069   BI580892   CO269885   CO272051   CO273154   CO293507   CO295146   CO302900   CO304592   CO311209   CO322776   CO329236   CO329564   EC064775   EC065713   EC070984   EC070985   EC072277   EC074307   EC090231   EC195958   EC197363   EC197841   EC209311   EC223547   EC224663   EC227740   EC234920   EC247167   GH751960   GH752129   GH753480   GH755071   GH755149   GH759058   GH759957   GH772042   GH777451   GH780468   GH784842   GH785612   GH786496   GH793522   GH804267   GH806069   GH806851   GH807287   GH810220   GH818800   GH819675   GH824334   GH825462   GH826510   GH829064   GH830924   GH834817   GH840516   GH844369   GH851582   GH853719   GH854981   GH856728   GH860019   GH864159   GH864918   GH865521   GH871525   GH873502   GH873973   GH878383   GH881264   GH882538   GH883068   GH884350   GH889563   GH896318   GH899075   GH901846   GH902171   GH904667   GH904790   GH906060   GH907126   GH908979   GH913155   GH914214   GH921478   GH927304   GH932137
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VBP2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 RASSF8GD11517 Other Phenotypes Allele decreased cell death RASSF86 decreased cell death | somatic clone RASSF86 increased cell number RASSF86 visible RASSF86 Phenotype manifest in Allele eye RASSF86 interommatidial bristle RASSF86 inter-ommatidial cell RASSF86 ommatidium RASSF86 wing RASSF86
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of RASSF8 Class Mutagen Stocks Known lesion RASSF8G15974 P-element activity 1 -- RASSF82 P-element activity 0 Yes RASSF84 P-element activity 0 Yes RASSF86 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RASSF8 Class Mutagen Stocks Known lesion RASSF8GD11517 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RASSF8JF02955 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RASSF8KK100500 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RASSF8Scer\UAS.P\T.cLa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3R)RASSF86 (Langton et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UASp-RASSF8.L} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}GE15974 NA P{EP}RASSF84 NA P{EP}RASSF8G15974 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
compound eye development inferred from mutant phenotype (Langton et al., 2009) dsRNA transport inferred from mutant phenotype (Saleh et al., 2006) negative regulation of multicellular organism growth inferred from mutant phenotype (Langton et al., 2009)
Cellular Component
CV term Evidence References
adherens junction inferred from direct assay (Langton et al., 2009)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
signal transduction inferred from electronic annotation with InterPro:IPR000159 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene (Langton et al., 2007) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RASSF8 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0261986 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43096
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16765 Dere\GG11418 Dgri\GH18985 Dmoj\GI22412 Dper\GL21526 Dpse\GA18623 Dsec\GM10258 Dsim\GD21229 Dvir\GJ10971 Dwil\GK12853 Dyak\GE23613
Curated drosophilid orthologs	Dpse\GA18623
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	31807 w1118; P{EP}RASSF8G15974 28323 y1 v1; P{TRiP.JF02955}attP2
VDRC	v105823 P{KK100500}VIE-260B v42019 w1118; P{GD11517}v42019
Genomic Clones ( 2 )
	BACR09D08 BACR48J06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 110 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH01133
Other clones	DPiM_GH01133
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT25531 AT26243 GH06784 GH13111 HL08116 LD01094 LD04640 LD15630 LD19134 LD33702 RE09742 RE31111 RE33048 RE49241 RE51141 RE66906 RE69479 RE70706 RE72787 RE74201 RE75139 SD07164
Other clones	7758 10206 28390 59426 61443 62938 73790 79084 101027 122186 190111 190950 CK01.79A.B10 CK01.110D.G1 CK01.129D.H9 EK096229 EK105725 EK109835 EK185122 EK188846 EK203148 EK208742 EK223744 EK245231 EK292326 EK293120 RP003001268 RP003012747 RP003024603 RP003034825 RP003041020 RP003046316 RP003051672 RP003058663 RP003061668 RP003088284 RP003090149 RP003092615 RP003092656 RP003112370 RP003125387 RP003129961 RP003133450 RP003133840 RP003139322 RP003142527 RP003170618 RP003191134 RP003195015 RP003200797 RP003207815 RP003208107 RP003210026 RP003211343 RP003216927 RP003217080 RP003219679 RP003271411 RP003276678 RP003280242 RP003299200 RP003306540 RP003306708 RP003312608 RP003314067 RP003314839 RP003337170 RP003343178 RP003377157 RP003377826 RP003395615 RP003407683 RP003416833 RP003426860 RP003442275 RP003445068 RP003459923 RP003465964 RP003483207 RP003486506 RP003533800 RP003538216 RP003559092 RP003588507 RP003654766 RP003658269
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0261986 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43096
Antibody Information
	polyclonal (Langton et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: RASSF8 CG5053 (Langton et al., 2009)
Source for database merge of	Source for merge of: RASSF8 Boa (FlyBase, 1992-)
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143154 RefSeq (Proteins) • NP_651411 DDBJ / EMBL / GenBank • AAF56488 AAK77232 AI062071 AW940024 AY047500 Entrez Gene - A searchable database of RefSeq genes. • 43096 UniProtKB/TrEMBL • Q9VBP2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ras-association (IPR000159)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0261986 DRSC - Results from RNAi screens. • FBgn0261986 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0261986 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43096 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43096 modMine - Data generated by the modENCODE project. • FBgn0261986
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Boa (Langton et al., 2007) CG5053 (Langton et al., 2009, Saleh et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009, Brandt, 2006) dRASSF8 (Langton et al., 2009) RASSF8
Name Synonym
Secondary FlyBase IDs
	FBgn0039352 FBgn0086725
References ( 15 )
Research paper	Langton et al., 2009, Curr. Biol. 19(23): 1969--1978 The dASPP-dRASSF8 complex regulates cell-cell adhesion during drosophila retinal morphogenesis. [FBrf0209661] Saleh et al., 2006, Nature Cell Biol. 8(8): 793--802 The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing. [FBrf0192229]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Brandt, 2006, Curr. Biol. 16(6): Supplemental Data. [FBrf0208876] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Bellen, 2010.7.14, BDGP_2010_0715.GenExel.xml BDGP_2010_0715.GenExel.xml [FBrf0211255] Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Perkins et al., 2009, Update to TRiP stock collection. Update to TRiP stock collection. [FBrf0208864] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Abstract	Langton et al., 2007, Europ. Dros. Res. Conf. 20: J006 DROSOPHILA ASPP IS A NOVEL REGULATOR OF C-TERMINAL SRC KINASE ACTIVITY. [FBrf0199887]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]