FlyBase Gene Report: Dmel\Gprk2

General Information

Symbol

Dmel\Gprk2

Species

D. melanogaster

Name

G protein-coupled receptor kinase 2

Annotation Symbol

CG17998

Feature Type

protein_coding_gene

FlyBase ID

FBgn0261988

Gene Model Status

Current

Stock Availability

22 publicly available

Enzyme Name (EC)

[G-protein-coupled receptor] kinase (2.7.11.16)

Gene Summary

G protein-coupled receptor kinase 2 (Gprk2) encodes a member of a family of serine/threonine kinases that modulate G-protein coupled receptors. It is required for female fertility, normal egg shape, and normal egg cAMP levels. It is also implicated in Hedgehog signaling and olfactory rhythmicity. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

nosh

Key Links

Genomic Location

Cytogenetic map

100C3-100C4

Sequence location

3R:31,405,245..31,457,873 [+]

Recombination map

3-102

RefSeq locus

NT_033777 REGION:31405245..31457873

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (20 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables G protein-coupled receptor kinase activity

inferred from mutant phenotype

(Schneider and Spradling, 1997)

enables phosphatidylinositol-4-phosphate binding

inferred from direct assay

(Jiang et al., 2016)

enables protein serine/threonine kinase activity

inferred from mutant phenotype

(Li et al., 2018, Li et al., 2016)

inferred from direct assay

(Giordano et al., 2018, Jiang et al., 2016, Maier et al., 2014, Chen et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000239, InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290, InterPro:IPR017441

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables protein kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN001219783

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (12 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in apical constriction involved in gastrulation

inferred from mutant phenotype

(Fuse et al., 2013)

involved_in defense response to Gram-positive bacterium

inferred from mutant phenotype

(Valanne et al., 2010)

involved_in imaginal disc-derived wing morphogenesis

inferred from mutant phenotype

(Chen et al., 2010, Molnar et al., 2007)

involved_in negative regulation of smoothened signaling pathway

inferred from genetic interaction with FLYBASE:fu; FB:FBgn0001079

(Cheng et al., 2012)

inferred from genetic interaction with FLYBASE:smo; FB:FBgn0003444

(Cheng et al., 2012)

inferred from genetic interaction with FLYBASE:ptc; FB:FBgn0003892

(Cheng et al., 2012)

inferred from genetic interaction with FLYBASE:Su(fu); FB:FBgn0005355

(Cheng et al., 2012)

involved_in oocyte morphogenesis

inferred from mutant phenotype

(Schneider and Spradling, 1997)

involved_in positive regulation of antibacterial peptide biosynthetic process

inferred from mutant phenotype

(Valanne et al., 2010)

involved_in positive regulation of protein kinase A signaling

inferred from genetic interaction with FLYBASE:Gαi; FB:FBgn0001104

(Cheng et al., 2012)

inferred from genetic interaction with FLYBASE:Gαs; FB:FBgn0001123

(Cheng et al., 2012)

involved_in positive regulation of receptor-mediated endocytosis

inferred from mutant phenotype

(Cheng et al., 2010)

involved_in positive regulation of smoothened signaling pathway

inferred from genetic interaction with FLYBASE:gish; FB:FBgn0250823

(Li et al., 2016)

inferred from mutant phenotype

(Giordano et al., 2018, Jiang et al., 2016, Ranieri et al., 2012, Cheng et al., 2010, Molnar et al., 2007)

inferred from direct assay

(Maier et al., 2014)

inferred from genetic interaction with FLYBASE:smo; FB:FBgn0003444

(Chen et al., 2010)

involved_in positive regulation of Toll signaling pathway

inferred from mutant phenotype

(Valanne et al., 2010)

inferred from genetic interaction with FLYBASE:Tl; FB:FBgn0262473

(Valanne et al., 2010)

involved_in protein phosphorylation

inferred from mutant phenotype

(Sopko et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in protein phosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN001219783

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in regulation of signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN001219785

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasmic side of plasma membrane

inferred from direct assay

(Jiang et al., 2016)

located_in cytoplasm

inferred from direct assay

(Schneider and Spradling, 1997)

located_in plasma membrane

inferred from high throughput direct assay

(Lye et al., 2014)

inferred from direct assay

(Schneider and Spradling, 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001219785

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

AGC KINASES (UNCLASSIFIED)

Pathway (FlyBase)

Positive Regulators of Hedgehog Signaling Pathway

Negative Regulators of Hedgehog Signaling Pathway

Positive Regulators of Toll-NF-κB Signaling Pathway

Protein Family (UniProt)

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. (P32866)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate (2.7.11.16)

Predictions / Assertions

Summaries

Gene Snapshot

Gene Group (FlyBase)

AGC KINASES (UNCLASSIFIED) -: The AGC kinase subfamily are serine/threonine kinases that were originally defined based on the sequence similarity of the catalytic domain. The AGC KINASE (UNCLASSIFIED) gene group represents AGC kinases that do not fall into the PKA, PKC or PKG families. (Adapted from FBrf0132098 and PMID:20027184).

Pathway (FlyBase)

Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Negative Regulators of Hedgehog Signaling Pathway -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
Positive Regulators of Toll-NF-κB Signaling Pathway -: In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).

Protein Function (UniProtKB)

Specifically phosphorylates the activated forms of G protein-coupled receptors (By similarity). Required during oogenesis and embryogenesis; component of a signaling pathway that functions during egg chamber maturation.

(UniProt, P32866)

Summary (Interactive Fly)

Serine/threonine protein kinase - involved in a receptor-mediated signaling pathway that utilizes cAMP as a second messenger - involved in oogenesis and implicated in learning

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Gprk2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Gprk2-RA

FBtr0085788

NM_057519

4690

714

Gprk2-RB

FBtr0334555

NM_001276214

3545

714

Additional Transcript Data and Comments

Reported size (kB)

5.5, 5.0, 4.0 (northern blot)

(Schneider and Spradling, 1997)

2.8 (longest cDNA)

(Cassill et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Gprk2-PA

FBpp0085149

80.7

714

6.31

NP_476867

AAF57152

Gprk2-PB

FBpp0306622

80.7

714

6.31

NP_001263143

AGB96522

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

714 aa isoforms: Gprk2-PA, Gprk2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

714 (aa); 80 (kD observed); 81 (kD predicted)

(Schneider and Spradling, 1997)

427 (aa); 50 (kD predicted)

(Cassill et al., 1991)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gprk2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gprk2_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

wing disc

(Molnar et al., 2007)

anterior-posterior compartment boundary of the wing disc | anterior to

(Molnar et al., 2007)

ventral thoracic disc

(Molnar et al., 2007)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Schneider and Spradling, 1997)

embryonic stage 1 -- 6

organism

(Schneider and Spradling, 1997)

third instar larval stage

(Schneider and Spradling, 1997)

oogenesis & adult stage | female

ovary

(Schneider and Spradling, 1997)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

antenna

Comment: exhibits circadian cycling

(Tanoue et al., 2008)

Additional Descriptive Data

Gprk2 is expressed in antennae. Gprk2 mRNA levels oscillate in a diurnal manner with a peak at ZT17 and a trough at ZT1.

(Tanoue et al., 2008)

Gprk2 transcript is expressed throughout the wing disc, with highest levels observed immediately anterior to the A/P boundary.

(Molnar et al., 2007)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

wing disc

(Schneider and Spradling, 1997)

embryonic/larval corpus allatum

(Schneider and Spradling, 1997)

mushroom body

(Schneider and Spradling, 1997)

embryonic/larval central nervous system

(Schneider and Spradling, 1997)

larval longitudinal connective

(Schneider and Spradling, 1997)

adult optic lobe

(Schneider and Spradling, 1997)

larval stage & adult stage

neuron

• axon

(Schneider and Spradling, 1997)

adult stage

adult mushroom body alpha-lobe

(Schneider and Spradling, 1997)

Kenyon cell

(Schneider and Spradling, 1997)

adult central nervous system

(Schneider and Spradling, 1997)

ellipsoid body

(Schneider and Spradling, 1997)

adult mushroom body | restricted

(Schneider and Spradling, 1997)

antenna

Comment: exhibits circadian cycling

(Tanoue et al., 2008)

oogenesis & adult stage | female

oocyte

• germinal vesicle

(Schneider and Spradling, 1997)

adult stage & oogenesis

germarium

(Schneider and Spradling, 1997)

follicle cell

(Schneider and Spradling, 1997)

ovary

(Schneider and Spradling, 1997)

nurse cell

(Schneider and Spradling, 1997)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Cassill et al., 1991)

third instar larval stage

wing disc

(Cheng et al., 2010)

adult stage

organism | ubiquitous

(Cassill et al., 1991)

adult head

(Cassill et al., 1991)

retina

(Cassill et al., 1991)

Additional Descriptive Data

Gprk2 is expressed in antennae. Gprk2 protein levels oscillate in a diurnal manner with a peak at ZT17 and a trough at ZT1.

(Tanoue et al., 2008)

Gprk1 protein is found at all stages by western blot analysis. Immunohistochemical staining shows that it is ubiquitous in the adult fly.

(Cassill et al., 1991)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasmic side of plasma membrane

inferred from direct assay

(Jiang et al., 2016)

located_in cytoplasm

inferred from direct assay

(Schneider and Spradling, 1997)

located_in plasma membrane

inferred from high throughput direct assay

(Lye et al., 2014)

inferred from direct assay

(Schneider and Spradling, 1997)

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.1}Gprk2^{MI01907-TG4.1}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Lee et al., 2018)

adult mushroom body

(Lee et al., 2018)

Reporter: P{ST.lexA::HG}PNPase^SE216

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

larval ventral nerve cord

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval central nervous system

(Bloomington Drosophila Stock Center, 2019)

Reporter: PBac{681.P.FSVS-1}Gprk2^CPTI002085

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult mushroom body

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Gprk2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Gprk2

View exonic expression by tissue for Dmel\Gprk2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB

49045/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0261988

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17998

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Gprk2

Transgenic constructs containing regulatory region of Gprk2

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3R)Exel6219

(Ryder, 2004.4.26)

Df(3R)Gprk2

(Cheng et al., 2010, Molnar et al., 2007)

Gene partially disrupted in

Df(3R)Gprk2-del1

(Cheng et al., 2010)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Gprk2^GD741

partially lethal | heat sensitive

Gprk2^Δ15

partially lethal - majority die | heat sensitive

Gprk2^KO

partially lethal - majority die | recessive

Gprk2⁰⁶⁹³⁶

viable

Sterility

Allele

female semi-sterile

Gprk2⁰⁶⁹³⁶

female semi-sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

female sterile | recessive

Gprk2⁰⁶⁹³⁶

fertile

Other Phenotypes

Allele

abnormal cell migration | embryonic stage

Gprk2⁰⁶⁹³⁶

abnormal cell migration | embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

Gprk2^UAS.cFa

abnormal immune response, with Scer\GAL4^c564

Gprk2^NIG.17998R

abnormal neurophysiology

abnormal neurophysiology, with Scer\GAL4^{Orco.2.642.Hsim\VP22}

Gprk2^UAS.cTa

decreased fecundity | female

Gprk2⁰⁶⁹³⁶

melanotic mass phenotype, with Scer\GAL4^{SPARC-MI00329-GAL4}

Gprk2^HMS00161

some die during embryonic stage | recessive | maternal effect | recessive

Gprk2⁰⁶⁹³⁶

some die during pupal stage, with Scer\GAL4^pnr-MD237

Gprk2^GD741

visible | heat sensitive

Phenotype manifest in

Allele

antenna

antenna, with Scer\GAL4^{Orco.2.642.Hsim\VP22}

Gprk2^UAS.cTa

anterior crossvein, with Scer\GAL4^MD-638

Gprk2^dsRNA.UAS

anterior crossvein, with Scer\GAL4^ptc-559.1

Gprk2^dsRNA.UAS

dorsal appendage

Gprk2⁰⁶⁹³⁶

dorsal appendage | germline clone

Gprk2⁰⁶⁹³⁶

egg

Gprk2⁰⁶⁹³⁶

egg chamber

Gprk2⁰⁶⁹³⁶

egg operculum

Gprk2⁰⁶⁹³⁶

embryo | embryonic stage 6

Gprk2⁰⁶⁹³⁶

embryo | gastrula stage

Gprk2⁰⁶⁹³⁶

embryo | maternal effect, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Gprk2^GL00233

embryonic/first instar larval cuticle | dorsal

Gprk2⁰⁶⁹³⁶

embryonic/first instar larval cuticle | ventral

Gprk2⁰⁶⁹³⁶

fat body, with Scer\GAL4^{SPARC-MI00329-GAL4}

Gprk2^HMS00161

germarium

Gprk2⁰⁶⁹³⁶

melanotic mass, with Scer\GAL4^{SPARC-MI00329-GAL4}

Gprk2^HMS00161

nurse cell

Gprk2⁰⁶⁹³⁶

nurse cell & actin filament

Gprk2⁰⁶⁹³⁶

nurse cell & nucleus

Gprk2⁰⁶⁹³⁶

nurse cell | germline clone

Gprk2⁰⁶⁹³⁶

oocyte

Gprk2⁰⁶⁹³⁶

primordial germ cell

Gprk2⁰⁶⁹³⁶

segment

Gprk2⁰⁶⁹³⁶

ventral furrow

Gprk2⁰⁶⁹³⁶

ventral furrow, with Scer\GAL4^{VP16.mat.αTub67C}

Gprk2^UAS.cFa

wing blade | heat sensitive

Gprk2^Δ15

wing cell, with Df(3R)Gprk2-del1

Gprk2^KO

wing cell | heat sensitive (with Df(3R)Gprk2)

Gprk2^KO

wing vein | heat sensitive

Gprk2^Δ15

wing vein L3, with Scer\GAL4^MD-638

Gprk2^dsRNA.UAS

wing vein L3, with Scer\GAL4^ptc-559.1

Gprk2^dsRNA.UAS

wing vein L3 | heat sensitive (with Df(3R)Gprk2)

Gprk2^KO

wing vein L4, with Scer\GAL4^MD-638

Gprk2^dsRNA.UAS

wing vein L4, with Scer\GAL4^ptc-559.1

Gprk2^dsRNA.UAS

wing vein L4 | heat sensitive (with Df(3R)Gprk2)

Gprk2^KO

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\GRK5

15 of 15

Yes

Hsap\GRK4

12 of 15

Yes

Hsap\GRK6

12 of 15

Yes

Hsap\GRK1

3 of 15

Yes

Hsap\GRK2

3 of 15

Hsap\GRK7

3 of 15

Yes

Hsap\GRK3

2 of 15

Hsap\ATP4B

1 of 15

Hsap\PTCHD3

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Grk5

15 of 15

Yes

Mmus\Grk6

12 of 15

Yes

Mmus\Grk4

11 of 15

Yes

Mmus\Grk1

4 of 15

Yes

Mmus\Grk2

3 of 15

Mmus\Grk3

2 of 15

Rattus norvegicus (Norway rat) (6)

Rnor\Grk5

13 of 13

Yes

Rnor\Grk4

11 of 13

Yes

Rnor\Grk6

10 of 13

Yes

Rnor\Grk1

4 of 13

Yes

Rnor\Grk2

3 of 13

Rnor\Grk3

1 of 13

Xenopus tropicalis (Western clawed frog) (7)

Xtro\grk4

12 of 12

Yes

Xtro\grk5

7 of 12

Yes

Xtro\grk6

7 of 12

Yes

Xtro\grk1

4 of 12

Yes

Xtro\grk5

3 of 12

Yes

Xtro\grk7

3 of 12

Yes

Xtro\grk3

1 of 12

Danio rerio (Zebrafish) (9)

Drer\grk4

13 of 15

Yes

Drer\grk6

13 of 15

Yes

Drer\grk5l

11 of 15

Yes

Drer\grk5

8 of 15

Yes

Drer\grk1a

4 of 15

Yes

Drer\grk1b

4 of 15

Yes

Drer\grk7a

3 of 15

Yes

Drer\grk7b

3 of 15

Yes

Drer\grk3

2 of 15

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\grk-1

15 of 15

Yes

Cele\grk-2

2 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\CPK20

1 of 9

Yes

Atha\S6K2

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919044X )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA14759

Drosophila persimilis

Dper\GL27250

Drosophila willistoni

Dwil\GK22764

Drosophila virilis

Dvir\GJ24327

Drosophila mojavensis

Dmoj\GI22206

Drosophila grimshawi

Dgri\GH19307

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502BI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17023637

Glossina morsitans

Tsetse fly

Gmor\GMOY012151

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11078

Mayetiola destructor

Hessian fly

Mdes\Mdes012098

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006110

Anopheles darlingi

American malaria mosquito

Adar\ADAC004602

Anopheles gambiae

Malaria mosquito

Agam\AGAP004117

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W042A )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA010747

Danaus plexippus

Monarch butterfly

Dple\DPOGS206290-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL013119

Apis florea

Little honeybee

Aflo\3705694436

Apis mellifera

Western honey bee

Amel\GB49466

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP18242

Bombus terrestris

Buff-tailed bumblebee

Bter\15210733

Bombus terrestris

Buff-tailed bumblebee

Bter\15202764

Linepithema humile

Argentine ant

Lhum\LH18187

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000a86

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000363

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12890

Tribolium castaneum

Red flour beetle

Tcas\TC011652

Pediculus humanus

Human body louse

Phum\PHUM066650

Rhodnius prolixus

Kissing bug

Rpro\RPRC007441

Cimex lectularius

Bed bug

Clec\CLEC002137

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006008

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_13749

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03YX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR012824

Ixodes scapularis

Black-legged tick

Isca\ISCW004541

Stegodyphus mimosarum

African social velvet spider

Smim\KFM77004.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur35g00320

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_127071

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G062G )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_011623gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Gprk3

Ciona intestinalis

Vase tunicate

Cint\LOC100186924

Gallus gallus

Domestic chicken

Ggal\GRK7

Gallus gallus

Domestic chicken

Ggal\GRK4

Gallus gallus

Domestic chicken

Ggal\GRK6

Gallus gallus

Domestic chicken

Ggal\GRK5

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

4 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

alcohol, response to, GRK4,5,6-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

GRK1; G protein-coupled receptor kinase 1

model of Oguchi disease-2

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Gprk2^UAS.cCb

exacerbates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(Chen and Feany, 2005)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GRK5; G protein-coupled receptor kinase 5

15 of 15

600870

GRK4; G protein-coupled receptor kinase 4

12 of 15

137026

GRK6; G protein-coupled receptor kinase 6

12 of 15

600869

GRK1; G protein-coupled receptor kinase 1

3 of 15

180381

OGUCHI DISEASE 2

Oguchi disease-2

GRK2; G protein-coupled receptor kinase 2

3 of 15

109635

GRK7; G protein-coupled receptor kinase 7

3 of 15

606987

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Gprk2

anti tag coimmunoprecipitation, anti tag western blot, fluorescent resonance energy transfer, fluorescence microscopy

(Chen et al., 2010)

Gprk2 - Cul4

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

Gprk2 - Gβ13F

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

Gprk2 - Gβ76C

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

Gprk2 - Smurf

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

Gprk2 - cact

anti tag coimmunoprecipitation, anti tag western blot

(Valanne et al., 2010)

Gprk2 - fu

anti tag coimmunoprecipitation, anti tag western blot

(Giordano et al., 2018)

Gprk2 - pic

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

Gprk2 - smo

fluorescent resonance energy transfer, fluorescence microscopy, pull down, anti tag western blot, anti tag coimmunoprecipitation

(Jiang et al., 2016, Chen et al., 2010)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Gprk2

suppressible

dnc

(Lannutti and Schneider, 2001)

Gprk2

suppressible

fog

(Fuse et al., 2013)

Gprk2

enhanceable

Pka-C1

(Cheng et al., 2012)

Gprk2

enhanceable

smo

(Molnar et al., 2007)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cta

suppressible

Gprk2

(Fuse et al., 2013)

dnc

suppressible

Gprk2

(Lannutti and Schneider, 2001)

fog

suppressible

Gprk2

(Chai et al., 2019)

gish|smo

enhanceable

Gprk2

(Li et al., 2016)

enhanceable

Gprk2

(Molnar et al., 2007)

smo

enhanceable

Gprk2

(Li et al., 2016, Chen et al., 2010)

suppressible

Gprk2

(Valanne et al., 2010)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261988

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

49045

MIST (genetic) - An integrated Molecular Interaction Database

Gprk2

MIST (protein-protein) - An integrated Molecular Interaction Database

Gprk2

Pathways

Signaling Pathways (FlyBase)

Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Negative Regulators of Hedgehog Signaling Pathway -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
Positive Regulators of Toll-NF-κB Signaling Pathway -: In Drosophila, the canonical Toll signaling pathway is initiated by the binding of a spatzle ligand to Toll (Tl) or a Toll-like receptor leading to the nuclear localization of the NF-κB (dl or Dif) transcription factor. Positive regulators of Toll-NF-κB Signaling lead to the increased nuclear accumulation of the NF-κB proteins and regulated expression of target genes. (Adapted from FBrf0091014 and FBrf0223077).

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Inactivation, recovery and regulation of the phototransduction cascade

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-102

Cytogenetic map

100C3-100C4

Sequence location

3R:31,405,245..31,457,873 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

100C3-100C4

Limits computationally determined from genome sequence between P{PZ}l(3)rM731^rM731 and P{EP}pygo^EP1076

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

100C3-100C5

(Schneider, 1997.5.19)

100C-100C

(determined by in situ hybridisation)

(Spradling et al., 1999, Schneider and Spradling, 1997)

100C-89E

(BDGP Project Members, 1994-1999)

100C3-100C5

(determined by in situ hybridisation)

(Cassill et al., 1991)

Experimentally Determined Recombination Data

Location

3-102

(FlyBase, 2016)

3-99.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (22)

Bloomington

44609

w^*; P{UAS-Gprk2.Cb}2

26097

w¹¹¹⁸; Mi{ET1}Gprk2^MB08070

41933

y¹ v¹; P{TRiP.HMS02330}attP40

44610

w^*; P{UAS-Gprk2.dsRNA}2; P{UAS-Su(fu).H}3

35954

y¹ w^*; Mi{MIC}Gprk2^MI01907

44184

y¹ w^*; Mi{MIC}Gprk2^MI07433

58038

y¹ w^*; Mi{MIC}Gprk2^MI13243

44608

w^*; P{neoFRT}82B Gprk2^R7.3/TM6B, Tb¹

Kyoto

115532

w¹¹¹⁸; PBac{681.P.FSVS-1}Gprk2^CPTI002051

115264

w¹¹¹⁸; PBac{681.P.FSVS-1}Gprk2^CPTI002085

VDRC

v101463

P{KK109241}VIE-260B

v1835

w¹¹¹⁸; P{GD741}v1835

More stocks available...

Genomic Clones (36)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (139)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0261988

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0261988

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

49045

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Cheng et al., 2010, Tanoue et al., 2008, Cassill et al., 1991)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Gprk2 CG17998

(Misra, 2000.4.8)

Source for merge of: Gprk2 nosh

(FlyBase, 1992-)

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Gprk2 has been cloned and sequenced, and its expression pattern has been analysed.

(Schneider and Spradling, 1997)

Mutations in nosh disrupt a pathway that is involved in the development of many different tissues. nosh protein is a putative G protein-coupled receptor kinase.

(Schneider and Spradling, 1995)

Gprk2 has been cloned and sequenced.

(Cassill et al., 1991)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

49045

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P32866

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KI69

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0261988

DRscDB

49045/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0261988

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gprk2_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17998

FlyMine - An integrated database for Drosophila genomics

FBgn0261988

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

49045

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011652

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG17998

MARRVEL_MODEL

49045

modMine - A data warehouse for the modENCODE project

FBgn0261988

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P32866

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0261988

DRSC - Results frm RNAi screens

FBgn0261988

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

49045

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

Gprk2

MIST (protein-protein) - An integrated Molecular Interaction Database

Gprk2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Inactivation, recovery and regulation of the phototransduction cascade

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

154978_at

(Kristensen et al., 2005)

CG17998

(Neuert et al., 2017, Hosono et al., 2015, Christis and Munro, 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Valanne et al., 2010, Deloger et al., 2009, Keleman et al., 2009.8.5, Krejcí et al., 2009, Mohit et al., 2006, Morrison et al., 2000)

GPRK2

(Galenza and Foley, 2019)

Gprk 2

(Bhadra et al., 2016)

Gprk-2

(Cassill et al., 1991)

Gprk2

(Hegazi et al., 2019, Kockel et al., 2019, Jha et al., 2018, Lee et al., 2018, Li et al., 2018, Li et al., 2018, Palmer et al., 2018, Gilmour et al., 2017, Neuert et al., 2017, Gene Disruption Project members, 2016-, Jiang et al., 2016, Lee et al., 2016, Gene Disruption Project members, 2015-, Zang et al., 2015, Abnave et al., 2014, Ashwal-Fluss et al., 2014, Garcia-Guerra et al., 2014, Sopko et al., 2014, Briscoe and Thérond, 2013, Chen and Jiang, 2013, Fuse et al., 2013, Hooper, 2013.5.30, Hooper, 2013.6.26, Mbodj et al., 2013, Cheng et al., 2012, Molnar et al., 2011, Toku et al., 2011, Chatterjee et al., 2010, Chen et al., 2010, Cheng et al., 2010, Raja et al., 2010, Valanne et al., 2010, Deloger et al., 2009, Krejcí et al., 2009, Telonis-Scott et al., 2009, Krishnan et al., 2008, Tanoue et al., 2008, Zhou et al., 2008, Molnar et al., 2007, Molnar et al., 2007, Chen and Feany, 2005)

gprk2

(Kang et al., 2020, Giordano et al., 2018, Li et al., 2016, Maier et al., 2014, Ranieri et al., 2014, Fuse et al., 2013, Cheng et al., 2012, Kind et al., 2008, Tanoue et al., 2006, Boyle et al., 2005, Lee et al., 2004, Fan and Schneider, 2003, Lannutti and Schneider, 2001, Schneider and Spradling, 1997)

nosh

Name Synonyms

G protein-coupled receptor kinase 2

(Garcia-Guerra et al., 2014, Chatterjee et al., 2010, Krishnan et al., 2008, Tanoue et al., 2008, Chen and Feany, 2005)

nosh

Secondary FlyBase IDs

FBgn0004834
FBgn0039847
FBgn0011677

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (128)

Report Sections

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