• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Sik3

General Information
Symbol	Dmel\Sik3	Species	D. melanogaster
Name	Salt-inducible kinase 3	Annotation symbol	CG42856
Feature type	protein_coding_gene	FlyBase ID	FBgn0262103
Gene Model Status	Current	Stock availability	14 publicly available
Also Known As	CG15072, CG18604
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	55E8-55E9	Sequence location	2R:14,575,637..14,593,310 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Salt-inducible kinase 3 is referred to in FlyBase by the symbol Dmel\Sik3 (CG42856, FBgn0262103). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: response to starvation; lipid homeostasis. 22 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Kinase associated domain 1 (KA1); Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Gene sequence location is 2R:14575637..14593310.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 55E8-55E9   Limits computationally determined from genome sequence between P{EP}EP437 and P{PZ}l(2)0370903709
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Sik3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Annotated transcripts do not represent all supported alternative splices within 5' UTR. Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Sik3-RA FBtr0304024  NM_137515   2983   702 Sik3-RB FBtr0304025  NM_137517   5637   1471 Sik3-RC FBtr0304026  NM_001202040   6469   1471 Sik3-RD FBtr0340388     6278   1468
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Sik3-PA   FBpp0292993   77.4   702   7.32     NP_611359   AAF57652 Sik3-PB   FBpp0292994   158.2   1471   6.75     NP_611361   AAF57651 Sik3-PC   FBpp0292995   158.2   1471   6.75     NP_001188969   ADV37215 Sik3-PD   FBpp0309334   157.9   1468   6.75
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Kinase associated domain 1 (KA1) (IPR001772)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA540249   AE013599 AAF57651   AE013599 AAF57652   AE013599 ADV37215   AW942539   AY061273 AAL28821   BE976183   BP547878   BP548967   BP548968   BT023509   CL943623   CO153935   CO307363   CO308843   CO322330   CO323434   CO327550   CO327561   CO328997   CZ482888   CZ484733   CZ484734   CZ485819   CZ487308   CZ487309   EC053516   EC053539   EC055357   EC055977   EC060897   EC062960   EC066139   EC086057   EC087397   EC200728   EC203219   EC235160   EC243815   EC258082   EC258489   EC260244   EC262754   EW712955   GH967924
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q4QQA7 A1ZBC4 Q7K243
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    9 head, virgin 1-day female    5 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    9 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    4 carcass, larvae L3 wandering    14 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    14 ovary, mated 4-day female    15 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    9 head, virgin 1-day female    5 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    9 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    4 carcass, larvae L3 wandering    14 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    14 ovary, mated 4-day female    15 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    9 head, virgin 1-day female    5 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    9 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    4 carcass, larvae L3 wandering    14 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    14 ovary, mated 4-day female    15 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    9 head, virgin 1-day female    5 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    9 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    4 carcass, larvae L3 wandering    14 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    14 ovary, mated 4-day female    15 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    9 head, virgin 1-day female    5 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    9 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    4 carcass, larvae L3 wandering    14 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    14 ovary, mated 4-day female    15 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    9 head, virgin 1-day female    5 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    9 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    4 carcass, larvae L3 wandering    14 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    14 ovary, mated 4-day female    15 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    9 head, virgin 1-day female    5 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    9 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    4 carcass, larvae L3 wandering    14 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    14 ovary, mated 4-day female    15 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    9 head, virgin 1-day female    5 head, virgin 4-day female    7 head, virgin 20-day female    7 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    7 head, mated 1-day male    9 head, mated 4-day male    8 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    6 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    4 carcass, larvae L3 wandering    14 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    14 ovary, mated 4-day female    15 testis, mated 4-day male    3 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    27 embryo 02-04hr    10 embryo 04-06hr    5 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    14 embryo 14-16hr    17 embryo 16-18hr    12 embryo 18-20hr    11 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    17 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    9 adult male 01day    8 adult male 05day    9 adult male 30day    10 adult female 01day    13 adult female 05day    17 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (27) embryo 02-04hr    10 embryo 04-06hr    5 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    14 embryo 14-16hr    17 embryo 16-18hr    12 embryo 18-20hr    11 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    17 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    9 adult male 01day    8 adult male 05day    9 adult male 30day    10 adult female 01day    13 adult female 05day    17 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    27 embryo 02-04hr    10 embryo 04-06hr    5 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    14 embryo 14-16hr    17 embryo 16-18hr    12 embryo 18-20hr    11 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    17 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    9 adult male 01day    8 adult male 05day    9 adult male 30day    10 adult female 01day    13 adult female 05day    17 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    27 embryo 02-04hr    10 embryo 04-06hr    5 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    14 embryo 14-16hr    17 embryo 16-18hr    12 embryo 18-20hr    11 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    17 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    9 adult male 01day    8 adult male 05day    9 adult male 30day    10 adult female 01day    13 adult female 05day    17 adult female 30day    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    27 embryo 02-04hr    10 embryo 04-06hr    5 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    14 embryo 14-16hr    17 embryo 16-18hr    12 embryo 18-20hr    11 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    17 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    9 adult male 01day    8 adult male 05day    9 adult male 30day    10 adult female 01day    13 adult female 05day    17 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  27 embryo 02-04hr    10 embryo 04-06hr    5 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    14 embryo 14-16hr    17 embryo 16-18hr    12 embryo 18-20hr    11 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    17 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    9 adult male 01day    8 adult male 05day    9 adult male 30day    10 adult female 01day    13 adult female 05day    17 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    27 embryo 02-04hr    10 embryo 04-06hr    5 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    14 embryo 14-16hr    17 embryo 16-18hr    12 embryo 18-20hr    11 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    17 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    9 adult male 01day    8 adult male 05day    9 adult male 30day    10 adult female 01day    13 adult female 05day    17 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    27 embryo 02-04hr    10 embryo 04-06hr    5 embryo 06-08hr    9 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    14 embryo 14-16hr    17 embryo 16-18hr    12 embryo 18-20hr    11 embryo 20-22hr    13 embryo 22-24hr    13 larva L1    10 larva L2    6 larva L3 12hr old    5 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    17 white prepupae new    20 white prepupae 12hr    25 white prepupae 24hr    17 pupae 2d postWPP    12 pupae 3d postWPP    5 pupae 4d postWPP    9 adult male 01day    8 adult male 05day    9 adult male 30day    10 adult female 01day    13 adult female 05day    17 adult female 30day    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    6 embryonic 1182-4H    12 embryonic GM2    8 embryonic Kc167    4 embryonic S1    5 embryonic S3    6 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    10 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    5 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    8 ovary fGS/OSS    7 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    6 embryonic 1182-4H    12 embryonic GM2    8 embryonic Kc167    4 embryonic S1    5 embryonic S3    6 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    10 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    5 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    8 ovary fGS/OSS    7 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    6 embryonic 1182-4H    12 embryonic GM2    8 embryonic Kc167    4 embryonic S1    5 embryonic S3    6 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    10 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    5 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    8 ovary fGS/OSS    7 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    6 embryonic 1182-4H    12 embryonic GM2    8 embryonic Kc167    4 embryonic S1    5 embryonic S3    6 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    10 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    5 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    8 ovary fGS/OSS    7 ovary OSC    6 ovary OSS    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    6 embryonic 1182-4H    12 embryonic GM2    8 embryonic Kc167    4 embryonic S1    5 embryonic S3    6 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    10 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    5 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    8 ovary fGS/OSS    7 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    6 embryonic 1182-4H    12 embryonic GM2    8 embryonic Kc167    4 embryonic S1    5 embryonic S3    6 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    10 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    5 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    8 ovary fGS/OSS    7 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    6 embryonic 1182-4H    12 embryonic GM2    8 embryonic Kc167    4 embryonic S1    5 embryonic S3    6 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    10 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    5 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    8 ovary fGS/OSS    7 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    6 embryonic 1182-4H    12 embryonic GM2    8 embryonic Kc167    4 embryonic S1    5 embryonic S3    6 leg disc CME L1    6 wing disc CME-W2    4 wing disc ML-DmD8    10 wing disc ML-DmD9    6 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    5 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    10 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    8 ovary fGS/OSS    7 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    7 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    5 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    9 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_27
External Data & Images
Linkouts	BDGP expression data • LD19671
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage Sik372 lethal | larval stage (with Df(2R)P34) Sik372 viable Sik348 Sik3EY14354 Sik3f03280 viable, with Scer\GAL4pnr-MD237 Sik3GD8535 Sik3GD9615 Sterility Allele fertile Sik3EY14354 Sik3f03280 Other Phenotypes Allele starvation stress response defective Sik348 Phenotype manifest in Allele
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of Sik3 Class Mutagen Stocks Known lesion Sik3f03280 piggyBac transposase 2 -- Sik3EY06260 1 -- Sik3EY14354 P-element activity 1 -- Sik3f00423 piggyBac transposase 1 -- Sik3f02224 piggyBac transposase 1 -- Sik3+51 P-element activity 0 Yes Sik348 P-element activity 0 Yes Sik372 P-element activity 0 Yes Sik3f04750 piggyBac transposase 0 -- Sik3SH0930 0 --
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Sik3 Class Mutagen Stocks Known lesion Sik3GD8535 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Sik3GD9615 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sik3GL00292 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sik3HMS01922 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sik3JF03002 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sik3KK104229 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sik3KK109965 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sik34A.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Sik3K70M.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Sik3NIG.15072R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Sik3Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Sik3T196A.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel7157 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.15072R} NA P{TRiP.HMS01922} NA P{UAS-Sik3.4A} NA P{UAS-Sik3.K70M} NA P{UAS-Sik3.T196A} NA P{UAS-Sik3.W} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Sik3EY06260 NA P{EPgy2}Sik3EY14354 NA miscellaneous insertions PBac{WH}Sik3f00423 NA PBac{WH}Sik3f02224 NA PBac{WH}Sik3f03280 NA PBac{WH}Sik3f04750 NA insertion of enhancer trap P{lacW}l(2)SH2156SH2156 No P{lacW}Sik3SH0930 No
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
lipid homeostasis inferred from mutant phenotype (Wang et al., 2011) response to starvation inferred from mutant phenotype (Wang et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001772, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein serine/threonine kinase activity inferred from electronic annotation with InterPro:IPR001772, InterPro:IPR008271 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000)
Biological Process
CV term Evidence References
protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001772, InterPro:IPR002290, InterPro:IPR008271 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Sik3 - CG9547 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Sik3 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262103 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37152 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q4QQA7
Orthologs
OrthoDB Orthologs (90) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG62BXV5 Dipteran inclusive ortholog search EOG68GWBG Insect inclusive ortholog search EOG6189H9 Arthropod inclusive ortholog search EOG63XSJD Metazoa inclusive ortholog search EOG6KHFMN
Orthologs in Drosophila Species (EOG62BXV5)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Sik3     Drosophila simulans   Dsim\GD11403 Y   Drosophila sechellia   Dsec\GM21908 Y   Drosophila erecta   Dere\GG21916 Y   Drosophila yakuba   Dyak\GE11992 Y   Drosophila ananassae   Dana\GF12120 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA24476 Y   Drosophila persimilis   Dper\GL10764 Y   Drosophila willistoni   Dwil\GK20917 Y   Drosophila virilis   Dvir\GJ20287 Y   Drosophila mojavensis   Dmoj\GI19215 Y   Drosophila grimshawi   Dgri\GH20038 Y
Orthologs in non-Drosophila Dipterans (EOG68GWBG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003061   Anopheles gambiae Malaria mosquito  Agam\AGAP006411
Orthologs in non-Dipteran Insects (EOG6189H9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB55293 Y Apis mellifera Western honey bee  Amel\LOC413736 Y Apis mellifera Western honey bee  Amel\GB53605 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001535 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG000160 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004357 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13277 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH26319 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH26320 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18522 Y Atta cephalotes Leafcutter ant  Acep\ACEP10312 Y Atta cephalotes Leafcutter ant  Acep\ACEP13906 Y Atta cephalotes Leafcutter ant  Acep\ACEP26575 Y Atta cephalotes Leafcutter ant  Acep\ACEP13905 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO16113 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO21082 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO21304 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL22113 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17246 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21967 Y Linepithema humile Argentine ant  Lhum\LH22412 Y Linepithema humile Argentine ant  Lhum\LH20896 Y Linepithema humile Argentine ant  Lhum\LH14613 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB17641 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB23352 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB22913 Y Solenopsis invicta Red fire ant  Sinv\SINV16206 Y Solenopsis invicta Red fire ant  Sinv\SINV13794 Y Solenopsis invicta Red fire ant  Sinv\SINV15562 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI009392 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI005190 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008317 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI56635 Y Bombyx mori Silkmoth  Bmor\BGIBMGA011370 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000136 Y Pediculus humanus Human body louse  Phum\PHUM368540 Y Pediculus humanus Human body louse  Phum\PHUM509230 Y Pediculus humanus Human body louse  Phum\PHUM287820 Y Tribolium castaneum Red flour beetle  Tcas\par-1 Y Tribolium castaneum Red flour beetle  Tcas\GLEAN_07481 Y Tribolium castaneum Red flour beetle  Tcas\GLEAN_15081 Y
Orthologs in non-Insect Arthropods (EOG63XSJD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\ATP6 Y Daphnia pulex Water flea  Dpux\BCORL1 Y Daphnia pulex Water flea  Dpux\JGI_V11_39834 Y Ixodes scapularis Deer tick  Isca\ISCW014579 Y Ixodes scapularis Deer tick  Isca\ISCW023145 Y Ixodes scapularis Deer tick  Isca\ISCW002853 Y
Orthologs in non-Arthropod Metazoa (EOG6KHFMN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\kin-29 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017316gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_015905gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000599gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019620gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007508gn Y Danio rerio Zebrafish  Drer\BX572077.2 Y Danio rerio Zebrafish  Drer\ENSDARG00000089170 Y Danio rerio Zebrafish  Drer\ENSDARG00000088083 Y Danio rerio Zebrafish  Drer\sik2a Y Danio rerio Zebrafish  Drer\si:ch211-235e18.3 Y Xenopus tropicalis Western clawed frog  Xtro\sik2 Y Xenopus tropicalis Western clawed frog  Xtro\sik3 Y Gallus gallus Domestic chicken  Ggal\SIK1 Y Gallus gallus Domestic chicken  Ggal\SIK2 Y Gallus gallus Domestic chicken  Ggal\SIK3 Y Mus musculus House mouse  Mmus\Sik3 Y Mus musculus House mouse  Mmus\ENSMUSG00000078792 Y Mus musculus House mouse  Mmus\Sik1 Y Mus musculus House mouse  Mmus\ENSMUSG00000090376 Y Mus musculus House mouse  Mmus\Sik2 Y Mus musculus House mouse  Mmus\Gm5156 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000043498 Y Rattus norvegicus Norway rat  Rnor\RGD1564651 Y Rattus norvegicus Norway rat  Rnor\LOC689939 Y Rattus norvegicus Norway rat  Rnor\Sik1 Y Homo sapiens Human  Hsap\SIK2 Y Homo sapiens Human  Hsap\SIK1 Y Homo sapiens Human  Hsap\ENSG00000160584 Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\SIK2     Hsap\SIK1     Hsap\ENSG00000160584
AAA Orthologs (22) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11403 Drosophila simulans Dsim\GD11404 Drosophila sechellia Dsec\GM21908 Drosophila sechellia Dsec\GM21909 Drosophila erecta Dere\GG21916 Drosophila erecta Dere\GG21918 Drosophila yakuba Dyak\GE11992 Drosophila yakuba Dyak\GE11993 Drosophila ananassae Dana\GF12120 Drosophila ananassae Dana\GF12121 Drosophila pseudoobscura pseudoobscura Dpse\GA24476 Drosophila pseudoobscura pseudoobscura Dpse\GA24477 Drosophila persimilis Dper\GL10764 Drosophila persimilis Dper\GL10765 Drosophila willistoni Dwil\GK20917 Drosophila willistoni Dwil\GK20918 Drosophila virilis Dvir\GJ20287 Drosophila virilis Dvir\GJ20288 Drosophila mojavensis Dmoj\GI19215 Drosophila mojavensis Dmoj\GI19216 Drosophila grimshawi Dgri\GH20038 Drosophila grimshawi Dgri\GH20039
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	18634 w1118; PBac{WH}Sik3f03280 28366 y1 v1; P{TRiP.JF03002}attP2 15962 y1 w67c23; P{EPgy2}Sik3EY06260 20921 y1 w67c23; P{EPgy2}Sik3EY14354
Harvard	- PBac{WH}Sik3f00423 - PBac{WH}Sik3f02224 - PBac{WH}Sik3f03280
VDRC	v106268 P{KK104229}VIE-260B v107458 P{KK109965}VIE-260B v20655 w1118; P{GD9615}v20655 v39864 w1118; P{GD8535}v39864 v39866 w1118; P{GD8535}v39866
	More stocks available...
Genomic Clones ( 1 )
	BACR08F09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 31 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD07105 LD19671
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	10476 30106 60964 63385 64541 64623 83684 123222 149335 214221.41 214539.58 214571.41 522963 bs47h03 EC053516 EC053539 EC055357 EC055977 EK187912 EK190450 EK254435 EK259508 EK291824 EN03133 EP07456 EP07521 FGH121F07 FGM002H05 RP004461429
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0262103 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37152
Antibody Information
	polyclonal (Wang et al., 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Sik3 CG42856 (Wang et al., 2011)
Source for database merge of	Source for merge of: CG15072 CG18604 (FlyBase Genome Annotators, 2010)
Additional comments	Annotations CG15072 and CG18604 merged as CG42856 in release 5.30 of the genome annotation. (FlyBase Genome Annotators, 2010)
Other Comments
	When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, spindle abnormalities, chromosome alignment defects are seen. (Bettencourt-Dias et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137515 NM_137517 NM_001202040 RefSeq (Proteins) • NP_611359 NP_611361 NP_001188969 DDBJ / EMBL / GenBank • AA540249 AAF57651 AAF57652 AAL28821 ABV53845 ABV53846 ADV37215 AW942539 AY061273 CL943623 CZ482888 CZ484733 CZ484734 CZ485819 CZ487308 CZ487309 Entrez Gene - A searchable database of RefSeq genes. • 37152 37154 UniProtKB/TrEMBL • A1ZBC4 Q4QQA7 Q7K243
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Kinase associated domain 1 (KA1) (IPR001772) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD19671
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262103 DRSC - Results from RNAi screens. • FBgn0262103 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0262103 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37152 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 37152 modMine - Data generated by the modENCODE project. • FBgn0262103 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q4QQA7
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG15072 (Amin et al., 2009, Wang et al., 2011, Keleman et al., 2009.8.5, Perkins et al., 2009, Liebl and Featherstone, 2005, Ni et al., 2011.7.19, Japanese National Institute of Genetics, 2012.5.21) CG18604 (Rehwinkel et al., 2006, Keleman et al., 2009.8.5) CG42856 (FlyBase Genome Annotators, 2010, Ni et al., 2010.12.1) EMK/KIAA0999 (Morrison et al., 2000) SIK (Amin et al., 2009) SIK3 (Wang et al., 2011) Sik3
Name Synonym	Salt-inducible kinase 3
Secondary FlyBase IDs
	FBgn0034376 FBgn0034378
References ( 30 )
Generate a list of	All references relating to this gene ( 30 )
List References by type	Research paper  ( 5 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 12 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 1 )
	Wang et al., 2011, Cell 145(4): 596--606 A hormone-dependent module regulating energy balance. [FBrf0213699] Show all research papers ...