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Gene Dmel\RanBPM

General Information
Symbol	Dmel\RanBPM	Species	D. melanogaster
Name	Ran-binding protein M	Annotation symbol	CG42236
Feature type	protein_coding_gene	FlyBase ID	FBgn0262114
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	CG11763
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	47A7-47A5	Sequence location	2R:6,317,213..6,324,086 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ran-binding protein M is referred to in FlyBase by the symbol Dmel\RanBPM (CG42236, FBgn0262114). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: Ran GTPase binding. There is experimental evidence that it is involved in the biological process: larval feeding behavior; ovarian follicle cell-cell adhesion; germ-line stem-cell niche homeostasis; JAK-STAT cascade; cytoskeleton organization; dorsal appendage formation. 14 alleles are reported. The phenotypes of these alleles are annotated with: terminal filament; ovary; germline stem cell; germarium cap cell; egg chamber. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: B30.2/SPRY domain; CRA domain; CTLH, C-terminal LisH motif; CTLH/CRA C-terminal to LisH motif domain; Concanavalin A-like lectin/glucanases superfamily; LisH dimerisation motif; SPla/RYanodine receptor SPRY; SPla/RYanodine receptor subgroup. Gene sequence location is 2R:6317213..6324086.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 47A7-47A5   Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 47A3-47A5   (determined by in situ hybridisation)   (Spradling et al., 1999) 47A3-47A5     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RanBPM for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Stop-codon suppression (UGA) postulated; FBrf0216884 gene_with_stop_codon_read_through ; SO:0000697 Gene model reviewed during 5.44 Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RanBPM-RA FBtr0088263  NM_165770   4370   960 RanBPM-RC FBtr0088262  NM_136741   3855   598 RanBPM-RD FBtr0100460  NM_001038849   4372   962 RanBPM-RE FBtr0306247  NM_001259326   4369   961 RanBPM-RF FBtr0330614  NM_001273938   3617   770
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RanBPM-PA   FBpp0087358   106.4   960   4.72     NP_724932   AAF58795 RanBPM-PC   FBpp0087357   66.4   598   6.67     NP_610585   AAM68758 RanBPM-PD   FBpp0099887   106.7   962   4.70     NP_001033938   ABC66064 RanBPM-PE   FBpp0297357   106.5   961   4.72     NP_001246255   AFH08009 RanBPM-PF   FBpp0303464   84.2   770   6.79     NP_001260867   AGB93400
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • CTLH/CRA C-terminal to LisH motif domain (IPR024964) B30.2/SPRY domain (IPR001870) LisH dimerisation motif (IPR006594) CRA domain (IPR013144) CTLH, C-terminal LisH motif (IPR006595) SPla/RYanodine receptor SPRY (IPR003877) SPla/RYanodine receptor subgroup (IPR018355) Concanavalin A-like lectin/glucanases superfamily (IPR008985)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141377   AA141459   AA949259   AA990795   AE013599 AAF58795   AE013599 AAM68758   AE013599 ABC66064   AE013599 AFH08009   AE013599 AGB93400   AI064041   AI135595   AI260695   AI296647   AQ025753   AY052091   AY069048   AY973544   AY973545   AY973546   BG632759   BG635680   BG637155   BI214081   BI230358   BI234853   BI237564   BI237575   BI240733   BI243022   BI364082   BI482023   BI482191   BI482982   BI483923   BI484459   BI485769   BI486310   BI573496   BI626851   BI633117   BI638301   BI638443   BP540407   BQ103488   BQ103738   BT011464   BT030999   BT083453   CK660044   CO177125   CO185387   CO188819   CO189683   CO272100   CO280467   CO290823   CO301304   CO302438   CO309068   CO313534   CO321544   CO326922   CO343552   CZ489352   CZ489353   EC057615   EC066331   EC068705   EC069962   EC080104   EC085527   EC087427   EC089344   EC194676   EC195664   EC217262   EC222248   EC223186   EC224901   EC232319   EC245599   EC248576   EC256899   EC257364   EC257830
UniProtKB/Swiss-Prot	Q4Z8K6
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference adult stage | female   ovary   (Dansereau and Lasko, 2008) oogenesis   terminal filament   (Dansereau and Lasko, 2008) follicle cell   (Dansereau and Lasko, 2008) germline stem cell   (Dansereau and Lasko, 2008) germarium cap cell   (Dansereau and Lasko, 2008) nurse cell   (Dansereau and Lasko, 2008)
Additional Descriptive Data
	RanBPM protein is expressed at the anterior tip of the germarium in the germline stem cells and in dividing cysts. Expression is reduced in the germline as individual egg chambers are formed. In later stages, it becomes enriched in the nuclei of follicle cells and germline cells and at the nurse cell membranes. The long RanBPM isoform (detected with an isoform-specific antibody) is detected in cap cells and in the terminal filament. (Dansereau and Lasko, 2008)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Scantlebury et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    62 central nervous system, pupae P8    63 head, virgin 1-day female    46 head, virgin 4-day female    45 head, virgin 20-day female    34 head, mated 1-day female    47 head, mated 4-day female    32 head, mated 20-day female    34 head, mated 1-day male    66 head, mated 4-day male    50 head, mated 20-day male    50 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    75 digestive system, larvae L3 wandering    101 digestive system, 1-day adult    27 digestive system, 4-day adult    29 digestive system, 20-day adult    36 fat body, larvae L3 wandering    30 fat body, white prepupae    55 fat body, pupae P8    34 carcass, larvae L3 wandering    81 carcass, 1-day adult    40 carcass, 4-day adult    30 carcass, 20-day adult    33 ovary, virgin 4-day female    70 ovary, mated 4-day female    78 testis, mated 4-day male    12 accessory gland, mated 4-day male    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (41) central nervous system, larvae L3  (62) central nervous system, pupae P8  (63) head, virgin 1-day female  (46) head, virgin 4-day female  (45) head, virgin 20-day female  (34) head, mated 1-day female  (47) head, mated 4-day female  (32) head, mated 20-day female  (34) head, mated 1-day male  (66) head, mated 4-day male  (50) head, mated 20-day male  (50) salivary gland, larvae L3 wandering    8 salivary gland, white prepupae  (75) digestive system, larvae L3 wandering  (101) digestive system, 1-day adult  (27) digestive system, 4-day adult  (29) digestive system, 20-day adult  (36) fat body, larvae L3 wandering  (30) fat body, white prepupae  (55) fat body, pupae P8  (34) carcass, larvae L3 wandering  (81) carcass, 1-day adult  (40) carcass, 4-day adult  (30) carcass, 20-day adult  (33) ovary, virgin 4-day female  (70) ovary, mated 4-day female  (78) testis, mated 4-day male    12 accessory gland, mated 4-day male  26 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    62 central nervous system, pupae P8    63 head, virgin 1-day female    46 head, virgin 4-day female    45 head, virgin 20-day female    34 head, mated 1-day female    47 head, mated 4-day female    32 head, mated 20-day female    34 head, mated 1-day male    66 head, mated 4-day male    50 head, mated 20-day male    50 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    75 digestive system, larvae L3 wandering  101 digestive system, 1-day adult    27 digestive system, 4-day adult    29 digestive system, 20-day adult    36 fat body, larvae L3 wandering    30 fat body, white prepupae    55 fat body, pupae P8    34 carcass, larvae L3 wandering    81 carcass, 1-day adult    40 carcass, 4-day adult    30 carcass, 20-day adult    33 ovary, virgin 4-day female    70 ovary, mated 4-day female    78 testis, mated 4-day male    12 accessory gland, mated 4-day male    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    62 central nervous system, pupae P8    63 head, virgin 1-day female    46 head, virgin 4-day female    45 head, virgin 20-day female    34 head, mated 1-day female    47 head, mated 4-day female    32 head, mated 20-day female    34 head, mated 1-day male    66 head, mated 4-day male    50 head, mated 20-day male    50 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    75 digestive system, larvae L3 wandering    101 digestive system, 1-day adult    27 digestive system, 4-day adult    29 digestive system, 20-day adult    36 fat body, larvae L3 wandering    30 fat body, white prepupae    55 fat body, pupae P8    34 carcass, larvae L3 wandering    81 carcass, 1-day adult    40 carcass, 4-day adult    30 carcass, 20-day adult    33 ovary, virgin 4-day female    70 ovary, mated 4-day female    78 testis, mated 4-day male    12 accessory gland, mated 4-day male    26 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    62 central nervous system, pupae P8    63 head, virgin 1-day female    46 head, virgin 4-day female    45 head, virgin 20-day female    34 head, mated 1-day female    47 head, mated 4-day female    32 head, mated 20-day female    34 head, mated 1-day male    66 head, mated 4-day male    50 head, mated 20-day male    50 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    75 digestive system, larvae L3 wandering    101 digestive system, 1-day adult    27 digestive system, 4-day adult    29 digestive system, 20-day adult    36 fat body, larvae L3 wandering    30 fat body, white prepupae    55 fat body, pupae P8    34 carcass, larvae L3 wandering    81 carcass, 1-day adult    40 carcass, 4-day adult    30 carcass, 20-day adult    33 ovary, virgin 4-day female    70 ovary, mated 4-day female    78 testis, mated 4-day male    12 accessory gland, mated 4-day male    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (41) central nervous system, larvae L3  (62) central nervous system, pupae P8  (63) head, virgin 1-day female  (46) head, virgin 4-day female  (45) head, virgin 20-day female  (34) head, mated 1-day female  (47) head, mated 4-day female  32 head, mated 20-day female  (34) head, mated 1-day male  (66) head, mated 4-day male  (50) head, mated 20-day male  (50) salivary gland, larvae L3 wandering    8 salivary gland, white prepupae  (75) digestive system, larvae L3 wandering  (101) digestive system, 1-day adult  27 digestive system, 4-day adult  29 digestive system, 20-day adult  (36) fat body, larvae L3 wandering  30 fat body, white prepupae  (55) fat body, pupae P8  (34) carcass, larvae L3 wandering  (81) carcass, 1-day adult  (40) carcass, 4-day adult  30 carcass, 20-day adult  (33) ovary, virgin 4-day female  (70) ovary, mated 4-day female  (78) testis, mated 4-day male    12 accessory gland, mated 4-day male  26 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    62 central nervous system, pupae P8    63 head, virgin 1-day female    46 head, virgin 4-day female    45 head, virgin 20-day female    34 head, mated 1-day female    47 head, mated 4-day female    32 head, mated 20-day female    34 head, mated 1-day male    66 head, mated 4-day male    50 head, mated 20-day male    50 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    75 digestive system, larvae L3 wandering  101 digestive system, 1-day adult    27 digestive system, 4-day adult    29 digestive system, 20-day adult    36 fat body, larvae L3 wandering    30 fat body, white prepupae    55 fat body, pupae P8    34 carcass, larvae L3 wandering    81 carcass, 1-day adult    40 carcass, 4-day adult    30 carcass, 20-day adult    33 ovary, virgin 4-day female    70 ovary, mated 4-day female    78 testis, mated 4-day male    12 accessory gland, mated 4-day male    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    41 central nervous system, larvae L3    62 central nervous system, pupae P8    63 head, virgin 1-day female    46 head, virgin 4-day female    45 head, virgin 20-day female    34 head, mated 1-day female    47 head, mated 4-day female    32 head, mated 20-day female    34 head, mated 1-day male    66 head, mated 4-day male    50 head, mated 20-day male    50 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    75 digestive system, larvae L3 wandering    101 digestive system, 1-day adult    27 digestive system, 4-day adult    29 digestive system, 20-day adult    36 fat body, larvae L3 wandering    30 fat body, white prepupae    55 fat body, pupae P8    34 carcass, larvae L3 wandering    81 carcass, 1-day adult    40 carcass, 4-day adult    30 carcass, 20-day adult    33 ovary, virgin 4-day female    70 ovary, mated 4-day female    78 testis, mated 4-day male    12 accessory gland, mated 4-day male    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    36 embryo 04-06hr    66 embryo 06-08hr    75 embryo 08-10hr    61 embryo 10-12hr    54 embryo 12-14hr    47 embryo 14-16hr    47 embryo 16-18hr    38 embryo 18-20hr    41 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    16 larva L2    13 larva L3 12hr old    14 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    60 white prepupae new    65 white prepupae 12hr    51 white prepupae 24hr    44 pupae 2d postWPP    35 pupae 3d postWPP    26 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    29 adult male 30day    26 adult female 01day    41 adult female 05day    47 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (75) embryo 02-04hr  (36) embryo 04-06hr  (66) embryo 06-08hr  (75) embryo 08-10hr  (61) embryo 10-12hr  (54) embryo 12-14hr  (47) embryo 14-16hr  (47) embryo 16-18hr  (38) embryo 18-20hr  (41) embryo 20-22hr  (29) embryo 22-24hr  (30) larva L1    16 larva L2    13 larva L3 12hr old    14 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6  (46) larva L3 puffstage 7-9  (60) white prepupae new  (65) white prepupae 12hr  (51) white prepupae 24hr  (44) pupae 2d postWPP  (35) pupae 3d postWPP  26 pupae 4d postWPP    23 adult male 01day  (31) adult male 05day  (29) adult male 30day  26 adult female 01day  (41) adult female 05day  (47) adult female 30day  (48) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    36 embryo 04-06hr    66 embryo 06-08hr    75 embryo 08-10hr    61 embryo 10-12hr    54 embryo 12-14hr    47 embryo 14-16hr    47 embryo 16-18hr    38 embryo 18-20hr    41 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    16 larva L2    13 larva L3 12hr old    14 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    60 white prepupae new    65 white prepupae 12hr    51 white prepupae 24hr    44 pupae 2d postWPP    35 pupae 3d postWPP    26 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    29 adult male 30day    26 adult female 01day    41 adult female 05day    47 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    36 embryo 04-06hr    66 embryo 06-08hr    75 embryo 08-10hr    61 embryo 10-12hr    54 embryo 12-14hr    47 embryo 14-16hr    47 embryo 16-18hr    38 embryo 18-20hr    41 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    16 larva L2    13 larva L3 12hr old    14 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    60 white prepupae new    65 white prepupae 12hr    51 white prepupae 24hr    44 pupae 2d postWPP    35 pupae 3d postWPP    26 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    29 adult male 30day    26 adult female 01day    41 adult female 05day    47 adult female 30day    48 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    36 embryo 04-06hr    66 embryo 06-08hr    75 embryo 08-10hr    61 embryo 10-12hr    54 embryo 12-14hr    47 embryo 14-16hr    47 embryo 16-18hr    38 embryo 18-20hr    41 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    16 larva L2    13 larva L3 12hr old    14 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    60 white prepupae new    65 white prepupae 12hr    51 white prepupae 24hr    44 pupae 2d postWPP    35 pupae 3d postWPP    26 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    29 adult male 30day    26 adult female 01day    41 adult female 05day    47 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (75) embryo 02-04hr  (36) embryo 04-06hr  (66) embryo 06-08hr  (75) embryo 08-10hr  (61) embryo 10-12hr  (54) embryo 12-14hr  (47) embryo 14-16hr  (47) embryo 16-18hr  (38) embryo 18-20hr  (41) embryo 20-22hr  29 embryo 22-24hr  30 larva L1    16 larva L2    13 larva L3 12hr old    14 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6  (46) larva L3 puffstage 7-9  (60) white prepupae new  (65) white prepupae 12hr  (51) white prepupae 24hr  (44) pupae 2d postWPP  (35) pupae 3d postWPP  26 pupae 4d postWPP    23 adult male 01day  31 adult male 05day  29 adult male 30day  26 adult female 01day  (41) adult female 05day  (47) adult female 30day  (48) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    36 embryo 04-06hr    66 embryo 06-08hr    75 embryo 08-10hr    61 embryo 10-12hr    54 embryo 12-14hr    47 embryo 14-16hr    47 embryo 16-18hr    38 embryo 18-20hr    41 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    16 larva L2    13 larva L3 12hr old    14 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    60 white prepupae new    65 white prepupae 12hr    51 white prepupae 24hr    44 pupae 2d postWPP    35 pupae 3d postWPP    26 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    29 adult male 30day    26 adult female 01day    41 adult female 05day    47 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    75 embryo 02-04hr    36 embryo 04-06hr    66 embryo 06-08hr    75 embryo 08-10hr    61 embryo 10-12hr    54 embryo 12-14hr    47 embryo 14-16hr    47 embryo 16-18hr    38 embryo 18-20hr    41 embryo 20-22hr    29 embryo 22-24hr    30 larva L1    16 larva L2    13 larva L3 12hr old    14 larva L3 puffstage 1-2    21 larva L3 puffstage 3-6    46 larva L3 puffstage 7-9    60 white prepupae new    65 white prepupae 12hr    51 white prepupae 24hr    44 pupae 2d postWPP    35 pupae 3d postWPP    26 pupae 4d postWPP    23 adult male 01day    31 adult male 05day    29 adult male 30day    26 adult female 01day    41 adult female 05day    47 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    78 Schneider line 2 Sg4    27 embryonic 1182-4H    67 embryonic GM2    72 embryonic Kc167    44 embryonic S1    85 embryonic S3    41 leg disc CME L1    35 wing disc CME-W2    27 wing disc ML-DmD8    32 wing disc ML-DmD9    55 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    25 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    51 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    32 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    64 tumorous blood cells mbn2    31 ovary fGS/OSS    82 ovary OSC    83 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (78) Schneider line 2 Sg4  (27) embryonic 1182-4H  (67) embryonic GM2  (72) embryonic Kc167  (44) embryonic S1  (85) embryonic S3  (41) leg disc CME L1  (35) wing disc CME-W2  (27) wing disc ML-DmD8  (32) wing disc ML-DmD9  (55) wing disc ML-DmD16-c3  (41) wing disc ML-DmD21    25 wing disc ML-DmD32  (44) haltere disc ML-DmD17-c3  (51) eye-antennal disc ML-DmD11  (35) antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1  (32) CNS ML-DmBG1-c1  (62) CNS ML-DmBG2-c2  (64) tumorous blood cells mbn2  (31) ovary fGS/OSS  (82) ovary OSC  (83) ovary OSS  (37) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    78 Schneider line 2 Sg4    27 embryonic 1182-4H    67 embryonic GM2    72 embryonic Kc167    44 embryonic S1    85 embryonic S3    41 leg disc CME L1    35 wing disc CME-W2    27 wing disc ML-DmD8    32 wing disc ML-DmD9    55 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    25 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    51 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    32 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    64 tumorous blood cells mbn2    31 ovary fGS/OSS    82 ovary OSC    83 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    78 Schneider line 2 Sg4    27 embryonic 1182-4H    67 embryonic GM2    72 embryonic Kc167    44 embryonic S1    85 embryonic S3    41 leg disc CME L1    35 wing disc CME-W2    27 wing disc ML-DmD8    32 wing disc ML-DmD9    55 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    25 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    51 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    32 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    64 tumorous blood cells mbn2    31 ovary fGS/OSS    82 ovary OSC    83 ovary OSS    37 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    78 Schneider line 2 Sg4    27 embryonic 1182-4H    67 embryonic GM2    72 embryonic Kc167    44 embryonic S1    85 embryonic S3    41 leg disc CME L1    35 wing disc CME-W2    27 wing disc ML-DmD8    32 wing disc ML-DmD9    55 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    25 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    51 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    32 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    64 tumorous blood cells mbn2    31 ovary fGS/OSS    82 ovary OSC    83 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (78) Schneider line 2 Sg4  27 embryonic 1182-4H  (67) embryonic GM2  (72) embryonic Kc167  (44) embryonic S1  (85) embryonic S3  (41) leg disc CME L1  (35) wing disc CME-W2  27 wing disc ML-DmD8  32 wing disc ML-DmD9  (55) wing disc ML-DmD16-c3  (41) wing disc ML-DmD21    25 wing disc ML-DmD32  (44) haltere disc ML-DmD17-c3  (51) eye-antennal disc ML-DmD11  (35) antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1  32 CNS ML-DmBG1-c1  (62) CNS ML-DmBG2-c2  (64) tumorous blood cells mbn2  31 ovary fGS/OSS  (82) ovary OSC  (83) ovary OSS  (37) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    78 Schneider line 2 Sg4    27 embryonic 1182-4H    67 embryonic GM2    72 embryonic Kc167    44 embryonic S1    85 embryonic S3    41 leg disc CME L1    35 wing disc CME-W2    27 wing disc ML-DmD8    32 wing disc ML-DmD9    55 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    25 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    51 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    32 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    64 tumorous blood cells mbn2    31 ovary fGS/OSS    82 ovary OSC    83 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    78 Schneider line 2 Sg4    27 embryonic 1182-4H    67 embryonic GM2    72 embryonic Kc167    44 embryonic S1    85 embryonic S3    41 leg disc CME L1    35 wing disc CME-W2    27 wing disc ML-DmD8    32 wing disc ML-DmD9    55 wing disc ML-DmD16-c3    41 wing disc ML-DmD21    25 wing disc ML-DmD32    44 haltere disc ML-DmD17-c3    51 eye-antennal disc ML-DmD11    35 antennal disc ML-DmD20-c5    15 mixed discs ML-DmD4-c1    32 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    64 tumorous blood cells mbn2    31 ovary fGS/OSS    82 ovary OSC    83 ovary OSS    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    55 cold shock, 4-day adult    42 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    20 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    69 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    29 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    25 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    72 Caffeine 25 mg/ml 48 hrs, 4-day adult    46 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    60 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (55) cold shock, 4-day adult  (42) heat shock, 4-day adult  (31) Cadmium 50 mM 6 hrs, larvae L3    20 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult  (40) Cadmium 100 mM 48 hrs, 4-day adult  (69) Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult  (40) Zinc 5 mM 12 hrs, larvae L3  (29) Zinc 4.5 mM 48 hrs, 4-day adult  (35) Ethanol 2.5% 3 hrs, larvae L3  (63) Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    25 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (72) Caffeine 25 mg/ml 48 hrs, 4-day adult  (46) Paraquat 5 mM 48 hrs, 4-day adult  (55) Paraquat 10 mM 48 hrs, 4-day adult  (60) Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    55 cold shock, 4-day adult    42 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    20 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    69 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    29 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    25 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    72 Caffeine 25 mg/ml 48 hrs, 4-day adult    46 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    60 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    55 cold shock, 4-day adult    42 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    20 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    69 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    29 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    25 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    72 Caffeine 25 mg/ml 48 hrs, 4-day adult    46 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    60 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    17 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    55 cold shock, 4-day adult    42 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    20 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    69 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    29 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    25 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    72 Caffeine 25 mg/ml 48 hrs, 4-day adult    46 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    60 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (55) cold shock, 4-day adult  (42) heat shock, 4-day adult  31 Cadmium 50 mM 6 hrs, larvae L3    20 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult  (40) Cadmium 100 mM 48 hrs, 4-day adult  (69) Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult  (40) Zinc 5 mM 12 hrs, larvae L3  29 Zinc 4.5 mM 48 hrs, 4-day adult  (35) Ethanol 2.5% 3 hrs, larvae L3  (63) Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    25 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (72) Caffeine 25 mg/ml 48 hrs, 4-day adult  (46) Paraquat 5 mM 48 hrs, 4-day adult  (55) Paraquat 10 mM 48 hrs, 4-day adult  (60) Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    55 cold shock, 4-day adult    42 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    20 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    69 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    29 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    25 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    72 Caffeine 25 mg/ml 48 hrs, 4-day adult    46 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    60 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    55 cold shock, 4-day adult    42 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    20 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    69 Copper 0.5 mM 12 hrs, larvae L3    25 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    29 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    63 Ethanol 5% 3 hrs, larvae L3    22 Ethanol 10% 3 hrs, larvae L3    14 Caffeine 1.5 mg/ml 4 hrs, larvae L3    25 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    72 Caffeine 25 mg/ml 48 hrs, 4-day adult    46 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    60 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • PC00172
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal RanBPMf06647 lethal (with Df(2R)12) RanBPMΔ lethal (with RanBPMf06647) RanBPMΔ lethal (with RanBPMΔ) RanBPMf06647 lethal | larval stage RanBPMk05201 lethal | recessive RanBPMk05201 lethal | temperature conditional RanBPMΔN lethal | temperature conditional (with RanBPMΔ) RanBPMΔN lethal | temperature conditional (with RanBPMΔN) RanBPMΔ partially lethal - majority die RanBPMCB-6232-3 partially lethal - majority die (with RanBPMCB-6232-3) RanBPMΔ partially lethal - majority die (with RanBPMΔ) RanBPMCB-6232-3 viable, with Scer\GAL4pnr-MD237 RanBPMGD6675 Sterility Allele semi-sterile RanBPMCB-6232-3 Other Phenotypes Allele cell shape defective RanBPMΔN cell size defective RanBPMΔN feeding behavior defective | third instar larval stage RanBPMk05201 RanBPMs135 increased cell size RanBPMΔ increased cell size (with RanBPMS.Scer\UAS), with Scer\GAL4arm.PS RanBPMΔ increased cell size (with RanBPMΔ), with Scer\GAL4arm.PS RanBPMS.Scer\UAS locomotor behavior defective | third instar larval stage RanBPMk05201 RanBPMts7 locomotor behavior defective | third instar larval stage (with RanBPMk05201) RanBPMs135 RanBPMts7 locomotor behavior defective | third instar larval stage (with RanBPMs135) RanBPMk05201 locomotor behavior defective | third instar larval stage (with RanBPMts7) RanBPMk05201 small body | third instar larval stage RanBPMk05201 RanBPMs135 Phenotype manifest in Allele egg chamber RanBPMCB-6232-3 RanBPMΔN RanBPMΔ egg chamber (with RanBPMCB-6232-3) RanBPMΔ egg chamber (with RanBPMΔ) RanBPMCB-6232-3 germarium cap cell RanBPMΔN RanBPMΔ germarium cap cell (with RanBPMS.Scer\UAS), with Scer\GAL4arm.PS RanBPMΔ germarium cap cell (with RanBPMΔ), with Scer\GAL4arm.PS RanBPMS.Scer\UAS germline stem cell RanBPMΔN RanBPMΔ germline stem cell (with RanBPMS.Scer\UAS), with Scer\GAL4arm.PS RanBPMΔ germline stem cell (with RanBPMΔ), with Scer\GAL4arm.PS RanBPMS.Scer\UAS ovary RanBPMCB-6232-3 RanBPMΔN ovary (with RanBPMCB-6232-3) RanBPMΔ ovary (with RanBPMΔ) RanBPMCB-6232-3 terminal filament RanBPMΔN RanBPMΔ terminal filament (with RanBPMS.Scer\UAS), with Scer\GAL4arm.PS RanBPMΔ terminal filament (with RanBPMΔ), with Scer\GAL4arm.PS RanBPMS.Scer\UAS
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of RanBPM Class Mutagen Stocks Known lesion RanBPM5-HA-1215 1 -- RanBPMCB-6232-3 hypomorphic allele - genetic evidence P-element activity 1 Yes RanBPMf06647 hypomorphic allele - genetic evidence piggyBac transposase 1 Yes RanBPMk05201 P-element activity 1 -- RanBPMNP6392 1 -- RanBPMDG09411 0 -- RanBPMrevertant P-element activity 0 Yes RanBPMs135 P-element activity 0 Yes RanBPMts7 0 Yes RanBPMΔ loss of function allele transposable element activity 0 Yes RanBPMΔN 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RanBPM Class Mutagen Stocks Known lesion RanBPMGD6675 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RanBPMlong.Scer\UAS in vitro construct - regulatory fusion 0 Yes RanBPMS.Scer\UAS in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)12 (Dansereau and Lasko, 2008, Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)stan2 (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Not disrupted in	Df(2R)X1 (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UAS-RanBPM.long} NA P{UAS-RanBPM.S} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{RS3}RanBPMCB-6232-3 NA P{RS5}RanBPM5-HA-1215 NA P{wHy}RanBPMDG09411 NA PBac{WH}RanBPMf06647 NA insertion of enhancer trap binary system P{GawB}RanBPMNP6392 No insertion of enhancer trap P{lacW}RanBPMk05201 No
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cytoskeleton organization inferred from direct assay (Dansereau and Lasko, 2008) dorsal appendage formation inferred from mutant phenotype (Dansereau and Lasko, 2008) germ-line stem-cell niche homeostasis inferred from mutant phenotype (Dansereau and Lasko, 2008) JAK-STAT cascade inferred from mutant phenotype (assigned by UniProtKB) (Baeg et al., 2005) larval feeding behavior inferred from mutant phenotype (Scantlebury et al., 2010) ovarian follicle cell-cell adhesion inferred from mutant phenotype (Dansereau and Lasko, 2008)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Scantlebury et al., 2010)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
Ran GTPase binding inferred from sequence or structural similarity with human RANBP9 (Curators, 2008--)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   gene_with_edited_transcript (Graveley et al., 2011) gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
RanBPM - CG11876 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RanBPM - CG14995 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) RanBPM - CG2183 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RanBPM - CG4151 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RanBPM - CG6227 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RanBPM - CG6617 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) RanBPM - CG6674 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RanBPM - CG7611 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) RanBPM - Csat anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RanBPM - lack anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RanBPM allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262114 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36102
Orthologs
OrthoDB Orthologs (47) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6T1J60 Dipteran inclusive ortholog search EOG6K3M4J Insect inclusive ortholog search EOG6QVB7B Arthropod inclusive ortholog search EOG6VDNDX Metazoa inclusive ortholog search EOG612ZZX
Orthologs in Drosophila Species (EOG6T1J60)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\RanBPM     Drosophila simulans   Dsim\GD10740     Drosophila sechellia   Dsec\GM21217     Drosophila erecta   Dere\GG24167     Drosophila yakuba   Dyak\GE19361     Drosophila ananassae   Dana\GF13762     Drosophila pseudoobscura pseudoobscura   Dpse\GA11183     Drosophila persimilis   Dper\GL17291     Drosophila virilis   Dvir\GJ22210     Drosophila mojavensis   Dmoj\GI19332     Drosophila grimshawi   Dgri\GH21253
Orthologs in non-Drosophila Dipterans (EOG6K3M4J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008645   Anopheles gambiae Malaria mosquito  Agam\AGAP005063   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000068
Orthologs in non-Dipteran Insects (EOG6QVB7B)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\RanBPM   Apis mellifera Western honey bee  Amel\GB53273   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG031080   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG024769   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12192   Atta cephalotes Leafcutter ant  Acep\ACEP18025   Camponotus floridanus Florida carpenter ant  Cflo\CFLO21975   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15053   Linepithema humile Argentine ant  Lhum\LH24760   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24401   Solenopsis invicta Red fire ant  Sinv\SINV18555   Acyrthosiphon pisum Pea aphid  Apim\ACYPI009438   Bombyx mori Silkmoth  Bmor\BGIBMGA004145   Bombyx mori Silkmoth  Bmor\BGIBMGA004146   Pediculus humanus Human body louse  Phum\PHUM378980   Tribolium castaneum Red flour beetle  Tcas\TC014542
Orthologs in non-Insect Arthropods (EOG6VDNDX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\RNF141   Daphnia pulex Water flea  Dpux\manx   Ixodes scapularis Deer tick  Isca\ISCW021328
Orthologs in non-Arthropod Metazoa (EOG612ZZX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\Y54E5A.7   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007153gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009648gn   Danio rerio Zebrafish  Drer\ranbp9   Danio rerio Zebrafish  Drer\ENSDARG00000062138   Xenopus tropicalis Western clawed frog  Xtro\ranbp9   Gallus gallus Domestic chicken  Ggal\RANBP10   Gallus gallus Domestic chicken  Ggal\RANBP9   Mus musculus House mouse  Mmus\Ranbp9   Mus musculus House mouse  Mmus\ENSMUSG00000037415   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000017951   Rattus norvegicus Norway rat  Rnor\Ranbp10   Homo sapiens Human  Hsap\RANBP9   Homo sapiens Human  Hsap\RANBP10
Human Orthologs (2)
Gene OMIM HGNC Hsap\RANBP9     Hsap\RANBP10
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	10564 y1 w67c23; P{lacW}RanBPMk05201/CyO
Harvard	- PBac{WH}RanBPMf06647
Kyoto	123990 w1118; P{RS3}RanBPMCB-6232-3 125146 w1118; P{RS5}RanBPM5-HA-1215 105228 y* w*; P{GawB}RanBPMNP6392 / CyO, P{UAS-lacZ.UW14}UW14
VDRC	v45981 w1118; P{GD6675}v45981
Genomic Clones ( 1 )
	BACR20O22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 76 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH05607 LP04379 RE27154 SD04057
Other clones	RE54051 RH09117 RH58540 RH61511
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH04291 LD28083 LD34676 LP04652 LP10618 LP18266 RE10155 RE19785 RE30621 RE33812 RE33824 RE37647 RE40549 RE49126 RE64663 RE64849 RE65794 RE66887 RE67563 RE69114 RE69727 RH09118 RH67924 SD13147 SD15025 SD20554 SD20734 SD27106
Other clones	8022 40408 50231 55941 58189 82764 84288 107712.2 157054 160386 214219.30 214539.40 214569.31 CK01.5C.A10 CK01.12B.C6 CK01.13A.E4 CK01.13A.E8 CK01.34D.E9 CK01.66C.G4 CK01.76C.H10 CK01652 CK01776 EK008934 EK042105 EK044903 EK052558 EK075302 EK105838 EK154135 EK180662 EK184145 EK185907 EK233319 EK260047 EP04963 EP17060 FGA004B02 GH13409 UUGC0894 UUGC1149
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0262114 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36102
Antibody Information
	polyclonal (Scantlebury et al., 2010, Dansereau and Lasko, 2008)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: RanBPM CG42236 (Dansereau and Lasko, 2008)
Source for database merge of	Source for merge of: RanBPM l(2)k05201 (Scantlebury et al., 2010)
Additional comments	The CG11763 annotation was originally named "midlife crisis (micr)" (see FBrf0182705) since the first alleles generated had a gradual germline stem cell (GSC) loss phenotype. A second site mutation has since been identified on the "micr" chromosomes, and it has been shown that loss of CG11763 does not cause a GSC loss phenotype. The second site mutations that cause GSC loss are referred to by the name "micr", while the alleles of CG11763 will be published under the gene name "Ran binding protein M (RanBPM)". (Dansereau, 2007.10.27) FlyBase curator comment: to prevent confusion, the CG11763 annotation has been renamed CG42236. (Dansereau, 2007.10.27)
Other Comments
	The long isoform of RanBPM functions in the germline stem cell niche to regulate its organisation and cell size. (Dansereau and Lasko, 2008) The short isoform of RanBPM functions in the germline stem cell niche to suppress adhesion between the cap cells and the germline stem cells. (Dansereau and Lasko, 2008) S2 cells treated with dsRNA against CG42236 display a significant increase in phospho-Stat92E nuclear accumulation upon os stimulation. (Baeg et al., 2005) S2-derived S2-NP cells are treated with dsRNA made from templates generated with primers directed against CG42236 to assess the role of this gene in Stat92E tyrosine phosphorylation. (Baeg et al., 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136741 NM_165770 NM_001038849 NM_001259326 NM_001273938 RefSeq (Proteins) • NP_610585 NP_724932 NP_001033938 NP_001246255 NP_001260867 DDBJ / EMBL / GenBank • AAF58795 AAM68758 ABC66064 AFH08009 AGB93400 AI260695 AQ025753 CZ489352 CZ489353 Entrez Gene - A searchable database of RefSeq genes. • 36102 UniProtKB/Swiss-Prot • Q4Z8K6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • CTLH/CRA C-terminal to LisH motif domain (IPR024964) B30.2/SPRY domain (IPR001870) LisH dimerisation motif (IPR006594) CRA domain (IPR013144) CTLH, C-terminal LisH motif (IPR006595) SPla/RYanodine receptor SPRY (IPR003877) SPla/RYanodine receptor subgroup (IPR018355) Concanavalin A-like lectin/glucanases superfamily (IPR008985) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • PC00172
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262114 DRSC - Results from RNAi screens. • FBgn0262114 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0262114 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36102 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36102 modMine - Data generated by the modENCODE project. • FBgn0262114
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG11763 (Dansereau et al., 2005, Dansereau et al., 2006, Scantlebury et al., 2006, Baeg et al., 2005, Alvarez, 2000.4.18, Giot et al., 2003, Dansereau, 2007.10.27, Dansereau and Lasko, 2008, Strub et al., 2008) CG42236 (Jungreis et al., 2011, Bridon et al., 2012) l(2)k05201   RanBPM (Scantlebury et al., 2010, Dansereau et al., 2006, Dansereau and Lasko, 2005.3.23, Dansereau and Lasko, 2005.3.23, Dansereau and Lasko, 2005.3.23, Dansereau, 2007.10.27, Dansereau and Lasko, 2008, Bridon et al., 2012)
Name Synonym	lethal (2) k05201   Ran-Binding Protein in the Microtubule Organizing Center (Scantlebury et al., 2010) Ran-binding protein M (Dansereau and Lasko, 2008) Ran binding protein M (Dansereau and Lasko, 2005.3.23, Dansereau and Lasko, 2005.3.23, Dansereau and Lasko, 2005.3.23, Dansereau, 2007.10.27) Ran-binding proteins 9/10 homologue (Bridon et al., 2012)
Secondary FlyBase IDs
	FBgn0022187 FBgn0033522 FBgn0250861
References ( 33 )
Generate a list of	All references relating to this gene ( 33 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 3 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 3 ) Website  ( 1 )
Recent research papers ( 2 )
	Adamson and Lajeunesse, 2012, ScientificWorldJournal 2012: 347597 A Study of Epstein-Barr Virus BRLF1 Activity in a Drosophila Model System. [FBrf0218446] Bridon et al., 2012, J. Proteome Res. 11(2): 927--940 Improvement of Phosphoproteome Analyses Using FAIMS and Decision Tree Fragmentation. Application to the Insulin Signaling Pathway in Drosophila melanogaster S2 Cells. [FBrf0217365] Show all research papers ...