dme-miR-iab-8, mir-iab-4as, iab-8, mir-iab8
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mir-iab-8 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\mir-iab-8 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
In addition to producing a miRNA (mir-iab-8) which represses abd-A expression, the 92kb iab-8 non-coding RNA has a second repressive function on abd-A expression which acts in cis, probably due to iab-8 transcription interfering with the abd-A promoter, which lies just downstream of the iab-8 poly(A) site.
The "iab-8" non-coding transcript encodes a microRNA (represented by "mir-iab-8") derived from a hairpin which is complementary to the hairpin present in the "iab-4" non-coding transcript (the hairpin in the "iab-4" transcript is represented by "mir-iab-4").
The "mir-iab-4" and "mir-iab-8" precursor hairpins are encoded on opposite strands of the same genomic region. The mature miRNAs derived from each hairpin have very similar sequences, but are shifted by 2 nucleotides at the 5' end and also differ in four positions at the 3' region.
The "mir-iab-4" and "mir-iab-8" precursor hairpins are encoded on opposite strands of the same genomic region. The major products of both hairpins are their respective "5p" species. While the 5' ends of the mature microRNA from each hairpin (mir-iab-4-5p and mir-iab-8-5p respectively) are related, the mir-iab-8-5p microRNA contains a strict 2 nucleotide "UU" extension.
The iab-8 ncRNA represses the expression of the abd-A gene in the posterior central nervous system. This repression is accomplished by two redundant mechanisms: firstly via the mir-iab-8 miRNA produced from the iab-8 transcript and secondly by a mechanism that acts only "in cis". The most likely explanation for second mechanism is that iab-8 transcription interferes with the abd-A promoter, which lies just downstream of the iab-8 poly(A) site.
New annotation CR42997 (which corresponds to mir-iab-8) in release 5.31 of the genome annotation.