FB2025_05 , released December 11, 2025
Gene: Dmel\mir-276b
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General Information
Symbol
Dmel\mir-276b
Species
D. melanogaster
Name
mir-276b stem loop
Annotation Symbol
CR42904
Feature Type
miRNA_gene
FlyBase ID
FBgn0262381
Gene Model Status
Current
Stock Availability
23 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

dme-mir-276b, mir-276bS, miR-276

Key Links
Genomic Location
Cytogenetic map
67E1-67E1
Sequence location
Recombination map
3-33
RefSeq locus
NT_037436 REGION:10319785..10319883
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables mRNA 3'-UTR binding
inferred from direct assay
(Zhang et al., 2021)
enables mRNA base-pairing post-transcriptional repressor activity
inferred from direct assay
(Zhang et al., 2021)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in miRNA-mediated post-transcriptional gene silencing
inferred from direct assay
(Zhang et al., 2021)
involved_in regulation of circadian sleep/wake cycle, sleep
inferred from mutant phenotype
(Zhang et al., 2021)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in miRNA-mediated post-transcriptional gene silencing
inferred from electronic annotation with Rfam:RF00637
(RNAcentral, 2018-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of RISC complex
inferred from electronic annotation with Rfam:RF00637
(RNAcentral, 2018-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Gene Model and Products
    Number of Transcripts
    3
    Number of Unique Polypeptides
    0

    Please see the JBrowse view of Dmel\mir-276b for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Structure
    Experimentally Determined Structures
    Crossreferences
    Comments on Gene Model

    Locations and structures of miRNA models as mapped by miRBase (FBrf0220601).

    Conserved: found throughout the Drosophila genus (FBrf0230987).

    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    mir-276b-RA
    FBtr0304226
    NR_048251
    23
    mir-276b-RB
    FBtr0304227
    NR_048252
    22
    mir-276b-RM
    FBtr0304225
    NR_048250
    99
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    miRBase - A searchable database of published miRNA sequences and annotation.
    Sequences Consistent with the Gene Model
    Nucleotide / Polypeptide Records
      Mapped Features

      Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mir-276b using the Feature Mapper tool.

      External Data
      Crossreferences
      Linkouts
      Expression Data
      Testis-specificity index

      The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

      NA

      Transcript Expression
      Additional Descriptive Data
      Marker for
       
      Subcellular Localization
      CV Term
      Polypeptide Expression
      Additional Descriptive Data
      Marker for
       
      Subcellular Localization
      CV Term
      Evidence
      References
      Expression Deduced from Reporters
      High-Throughput Expression Data
      Associated Tools

      JBrowse - Visual display of RNA-Seq signals

      View Dmel\mir-276b in JBrowse
      RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
      Bulk Downloads
      RNA-Seq RPKM values for all genes
      Reference
      See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
      Developmental Proteome: Life Cycle
      Developmental Proteome: Embryogenesis
      External Data and Images
      Linkouts
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      Images
      FlyBase Wiki
      Image Based Resources
      Alleles, Insertions, Transgenic Constructs, and Aberrations
      Classical and Insertion Alleles ( 2 )
      For All Classical and Insertion Alleles Show
       
      Other relevant insertions
      Transgenic Constructs ( 7 )
      For All Alleles Carried on Transgenic Constructs Show
      Transgenic constructs containing/affecting coding region of mir-276b
      Transgenic constructs containing regulatory region of mir-276b
      Aberrations (Deficiencies and Duplications) ( 0 )
      Inferred from experimentation ( 0 )
      Inferred from location ( 1 )
      Variants
      Variant Molecular Consequences
      Alleles Representing Disease-Implicated Variants
      Phenotypes
      For more details about a specific phenotype click on the relevant allele symbol.
      Lethality
      Allele
      lethal, with Scer\GAL4Act5C.PU
      lethal, with Scer\GAL4da.PU
      partially lethal | recessive
      viable, with Scer\GAL4Tub.PU
      Sterility
      Allele
      female semi-sterile
      male fertile
      Other Phenotypes
      Allele
      abnormal circadian behavior | adult stage
      abnormal circadian behavior | adult stage, with Scer\GAL4mir-276b-KIG
      abnormal circadian rhythm | adult stage
      abnormal locomotor behavior | adult stage | recessive
      abnormal neuroanatomy | larval stage, with Scer\GAL4ppk.PG
      decreased sleep, with Scer\GAL4GMR65C12
      decreased sleep, with Scer\GAL4GMR72B09
      decreased sleep, with Scer\GAL4mir-276b-KIG
      decreased sleep, with Scer\GAL4tim.PE
      increased sleep
      short lived | female | recessive
      short lived | male | recessive
      some die during embryonic stage | recessive | maternal effect | recessive
      visible, with Scer\GAL4Bx-MS1096
      visible, with Scer\GAL4sd-SG29.1
      visible | male limited, with Scer\GAL4sd-SG29.1
      Phenotype manifest in
      Allele
      adult heart
      adult heart, with Scer\GAL4Cg.PA
      dendrite | larval stage, with Scer\GAL4ppk.PG
      larval multidendritic class IV neuron, with Scer\GAL4ppk.PG
      wing, with Scer\GAL4Bx-MS1096
      wing, with Scer\GAL4sd-SG29.1
      wing | male limited, with Scer\GAL4sd-SG29.1
      wing margin bristle, with Scer\GAL4sd-SG29.1
      Orthologs
      Downloads
      Download All DIOPT Orthologs
      Human Orthologs (via DIOPT v9.1)
      Species\Gene Symbol
      Score
      Best Score
      Best Reverse Score
      Source
      Compara
      Domainoid
      eggNOG
      Hieranoid
      Homologene
      Inparanoid
      OMA
      OrthoDB
      OrthoFinder
      OrthoInspector
      orthoMCL
      Panther
      Phylome
      SonicParanoid
      Alignment
      Complementation?
      Transgene?
      Homo sapiens (Human) (0)
      Model Organism Orthologs (via DIOPT v9.1)
      Species\Gene Symbol
      Score
      Best Score
      Best Reverse Score
      Source
      Compara
      Domainoid
      eggNOG
      Hieranoid
      Homologene
      Inparanoid
      OMA
      OrthoDB
      OrthoFinder
      OrthoInspector
      orthoMCL
      Panther
      Phylome
      SonicParanoid
      Alignment
      Complementation?
      Transgene?
      Rattus norvegicus (Norway rat) (0)
      Mus musculus (laboratory mouse) (0)
      Xenopus tropicalis (Western clawed frog) (0)
      Danio rerio (Zebrafish) (0)
      Caenorhabditis elegans (Nematode, roundworm) (0)
      Anopheles gambiae (African malaria mosquito) (0)
      Arabidopsis thaliana (thale-cress) (0)
      Saccharomyces cerevisiae (Brewer's yeast) (0)
      Schizosaccharomyces pombe (Fission yeast) (0)
      Escherichia coli (enterobacterium) (0)
      Other Organism Orthologs (via OrthoDB)
      Data provided directly from OrthoDB:mir-276b. Refer to their site for version information.
      Paralogs
      Downloads
      Download All DIOPT Paralogs
      Paralogs (via DIOPT v9.1)
      Human Disease Associations
      FlyBase Human Disease Model Reports
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Allele
        Disease
        Evidence
        References
        Potential Models Based on Orthology ( 0 )
        Human Ortholog
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Allele
        Disease
        Interaction
        References
        Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
        Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
        Homo sapiens (Human)
        Gene name
        Score
        OMIM
        OMIM Phenotype
        DO term
        Complementation?
        Transgene?
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        mir-276b
        suppressible
        nej
        (Li and Gavis, 2022)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Fmr1
        suppressible
        mir-276b
        (Li and Gavis, 2022)
        nej
        suppressible
        mir-276b
        (Li and Gavis, 2022)
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-33
        Cytogenetic map
        67E1-67E1
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        67E1-67E1
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-33

        (FlyBase, 2016)

        3-25.1

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (23)
        Genomic Clones (22)
        cDNA Clones (0)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
          Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
                Other clones
                  RNAi and Array Information
                  Linkouts
                  Antibody Information
                  Laboratory Generated Antibodies
                   
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                      Other Comments

                      New annotation (CR33586) in release 4.1 of the genome annotation.

                      (FlyBase Genome Annotators, 2005)

                      New annotation (CR33587) in release 4.1 of the genome annotation.

                      (FlyBase Genome Annotators, 2005)
                      Relationship to Other Genes
                      Source for database merge of

                      Source for merge of: mir-276b mir-276bS

                      (FlyBase Genome Annotators, 2010)
                      Additional comments

                      The annotation for mir-276b has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. Thus the separate annotations (CR33586 and CR33587) which previously each represented one of the two mature miRNAs made from the mir-276b stem loop precursor have been converted into a single annotation (CR42904).

                      (FlyBase Genome Annotators, 2010)
                      Nomenclature History
                      Source for database identify of
                      Nomenclature comments
                      Etymology
                      Synonyms and Secondary IDs (14)
                      Reported As
                      Symbol Synonym
                      CR33586
                      (FlyBase Genome Annotators, 2005)
                      CR33587
                      (FlyBase Genome Annotators, 2005)
                      CR42904
                      (FlyBase Genome Annotators, 2010)
                      MIR-276
                      (Ylla et al., 2016)
                      MiR-276b
                      (Yang et al., 2009)
                      Mir-276b
                      (Robins et al., 2005)
                      dme-miR-276b
                      (Volin et al., 2018, Towler et al., 2015, Yang et al., 2008)
                      dme-mir-276
                      (Krishnan et al., 2006)
                      dme-mir-276b
                      (He et al., 2022, Mohammed et al., 2014, miRBase, 2013, Berezikov et al., 2011, Wang and Liu, 2011)
                      miR-276
                      (Kadener et al., 2009, Leaman, 2005)
                      miR-276b
                      (Li et al., 2024, Tang et al., 2024, Ramesh et al., 2023, Yang et al., 2023, Xia et al., 2020, Atilano et al., 2017, Picao-Osorio et al., 2017, Rainey et al., 2016, Cook and Van Vactor, 2015.6.15, Fulga et al., 2015, Suh et al., 2015, Abe et al., 2014, Esslinger et al., 2013, Mezan et al., 2013, Nesler et al., 2013, Liu et al., 2012, Liu et al., 2011, Ghildiyal et al., 2010, Ibáñez-Ventoso et al., 2008, Behura, 2007, Stark et al., 2005, Nakahara and Carthew, 2004, Rehmsmeier et al., 2004, Lai et al., 2003, Stark et al., 2003, Stark et al., 2003)
                      mir-276b
                      (Örkenby et al., 2023, Li and Gavis, 2022, Kitatani et al., 2020, Mohammed and Lai, 2016.2.8, Chen et al., 2014, Wang et al., 2014, Yeh et al., 2014, He et al., 2013, Bejarano et al., 2012, Schertel et al., 2012, Szuplewski et al., 2012, FlyBase Genome Annotators, 2010, Neumuller et al., 2008, Okamura et al., 2008, Ruby et al., 2007)
                      mir-276bS
                      Name Synonyms
                      miR-276b
                      (Behura, 2007, Rehmsmeier et al., 2004)
                      mir-276b
                      mir-276b stem loop
                      mir-276bS
                      Secondary FlyBase IDs
                      • FBgn0067716
                      • FBgn0082179
                      • FBgn0262197
                      Datasets (0)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (0)
                      Result
                      Result Type
                      Title
                      External Crossreferences and Linkouts ( 15 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      RNAcentral - A comprehensive ncRNA sequence collection representing all ncRNA types from a broad range of organisms
                      Other crossreferences
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      miRBase - A searchable database of published miRNA sequences and annotation.
                      Linkouts
                      DroID - A comprehensive database of gene and protein interactions.
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      References (72)