• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\mir-306

General Information
Symbol	Dmel\mir-306	Species	D. melanogaster
Name	mir-306 stem loop	Annotation symbol	CR42914
Feature type	miRNA_gene	FlyBase ID	FBgn0262386
Gene Model Status	Current	Stock availability	1 publicly available
Also Known As	dme-mir-306, miR-306S
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	36A10-36A10	Sequence location	2L:16,698,404..16,698,488 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene mir-306 stem loop is referred to in FlyBase by the symbol Dmel\mir-306 (CR42914, FBgn0262386). It is a miRNA_gene from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 5 alleles are reported. The phenotypes of these alleles are annotated with: eye; spermatocyte cyst; wing. It has one annotated transcript. Gene sequence location is 2L:16698404..16698488.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 36A10-36A10
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mir-306 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Locations and structures of miRNA models as mapped by miRBase (FBrf0220601).
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt)   mir-306-RM FBtr0304247  NR_047969   85   mir-306-RA FBtr0304248  NR_047970   22   mir-306-RB FBtr0304249  NR_047971   22
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences	miRBase - the home of microRNA data • MI0000414
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence   Name
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4Act5C.PU mir-306Scer\UAS.cSa Other Phenotypes Allele visible, with Scer\GAL4Bx-MS1096 mir-306Scer\UAS.cSa Phenotype manifest in Allele eye, with Scer\GAL4ey.PU mir-306Scer\UAS.cSa spermatocyte cyst, with Scer\GAL4nos.UTR.T:Hsim\VP16 mir-306Scer\UAS.cEa wing, with Scer\GAL4Bx-MS1096 mir-306Scer\UAS.cSa
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of mir-306 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mir-306 Class Mutagen Stocks Known lesion mir-306Scer\UAS.cluster.T:Ppyr\LUC in vitro construct 1 Yes mir-306Scer\UAS.cEa in vitro construct - regulatory fusion 0 Yes mir-306Scer\UAS.cluster.cLa in vitro construct - regulatory fusion 0 Yes mir-306Scer\UAS.cluster.T:Disc\RFP in vitro construct 0 Yes mir-306Scer\UAS.cSa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct M{UAS-mir-306.S} NA P{UAS-DsRed-mir-9c+mir-306+mir-79+mir-9b.L} NA P{UAS-LUC-mir-9c.mir-306.mir-79.mir-9b.T} NA P{UAS-mir-9c+mir-306+mir-79+mir-9b.L} NA P{UAS-mir-306.E} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	miRNA_gene (Stark et al., 2003, Stark et al., 2003, FlyBase Genome Annotators, 2010) nuclear_gene (Stark et al., 2003, Stark et al., 2003, FlyBase Genome Annotators, 2010)
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mir-306 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262386
Orthologs
OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search No orthologs identified Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (None identified)
No orthologies identified
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
Bloomington	41156 w1118; P{UAS-LUC-mir-9c.mir-306.mir-79.mir-9b.T}attP2
Genomic Clones ( 1 )
	BACR26O03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 0 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 12798237
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: mir-306 mir-306S (FlyBase Genome Annotators, 2010)
Additional comments	The annotation for mir-306 has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. Thus the separate annotations (CR33609 and CR33610) which previously each represented one of the two mature miRNAs made from the mir-306 stem loop precursor have been converted into a single annotation (CR42914). (FlyBase Genome Annotators, 2010)
Other Comments
	New annotation (CR33609) in release 4.1 of the genome annotation. (FlyBase Genome Annotators, 2005) New annotation (CR33610) in release 4.1 of the genome annotation. (FlyBase Genome Annotators, 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NR_047969 NR_047970 NR_047971 Entrez Gene - A searchable database of RefSeq genes. • 3772536 12798237
Other Crossreferences
	miRBase - the home of microRNA data • MI0000414
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262386 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0262386 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 12798237 modMine - Data generated by the modENCODE project. • FBgn0262386
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	306 (Han et al., 2011) CR33609 (FlyBase Genome Annotators, 2005) CR33610 (FlyBase Genome Annotators, 2005) CR42914 (FlyBase Genome Annotators, 2010, ) dme-mir-306 (Ryazansky et al., 2011, Krishnan et al., 2006, Huang and Gu, 2007) miR-306 (Liu et al., 2011, Leaman, 2005, Stark et al., 2003, Stark et al., 2003, Stark et al., 2005, Pek et al., 2009, Czech et al., 2009, Tsurudome et al., 2010, Behura et al., 2011, Ibáñez-Ventoso et al., 2008, Eun et al., 2013, Liu et al., 2012) mir-306 (Daneshvar et al., 2013, Okamura et al., 2008, FlyBase Genome Annotators, 2010, Biryukova et al., 2007, Ravulapalli et al., 2008, Neumuller et al., 2008, Okamura et al., 2009, Herranz et al., 2010, Ruby et al., 2007, Schertel et al., 2012, Burgler and Macdonald, 2005, Bejarano et al., 2012) MiR-306 (Yang et al., 2009) mir-306* (Aravin et al., 2003) miR-306S (Stark et al., 2003, Stark et al., 2003) mir-306S
Name Synonym	mir-306   mir-306S   mir-306 stem loop
Secondary FlyBase IDs
	FBgn0067698 FBgn0067699 FBgn0262207
References ( 33 )
Generate a list of	All references relating to this gene ( 33 )
List References by type	Research paper  ( 23 ) Supplementary material  ( 5 ) Abstract  ( 3 ) FlyBase analysis  ( 2 )
Recent research papers ( 9 )
	Daneshvar et al., 2013, Biol. Open 2(1): 1--9 MicroRNA miR-308 regulates dMyc through a negative feedback loop in Drosophila. [FBrf0220584] Eun et al., 2013, Development 140(1): 23--30 MicroRNAs downregulate Bag of marbles to ensure proper terminal differentiation in the Drosophila male germline. [FBrf0220183] Liu et al., 2012, Nature 482(7386): 519--523 The microRNA miR-34 modulates ageing and neurodegeneration in Drosophila. [FBrf0217556] Marrone et al., 2012, BMC Cell Biol. 13: 26 Dg-Dys-Syn1 signaling in Drosophila regulates the microRNA profile. [FBrf0220581] Schertel et al., 2012, Genetics 192(4): 1543--1552 Functional Characterization of Drosophila microRNAs by a Novel in Vivo Library. [FBrf0220194] Behura et al., 2011, PLoS ONE 6(7): e21504 Comparative Genomic Analysis of Drosophila melanogaster and Vector Mosquito Developmental Genes. [FBrf0214308] Han et al., 2011, Curr. Biol. 21(22): 1878--1887 The 3'-to-5' Exoribonuclease Nibbler Shapes the 3' Ends of MicroRNAs Bound to Drosophila Argonaute1. [FBrf0216729] Liu et al., 2011, Curr. Biol. 21(22): 1888--1893 The Exoribonuclease Nibbler Controls 3' End Processing of MicroRNAs in Drosophila. [FBrf0216782] Ryazansky et al., 2011, BMC Genomics 12: 371 Evidence for post-transcriptional regulation of clustered microRNAs in Drosophila. [FBrf0214628] Show all research papers ...