FB2025_01 , released February 20, 2025
Gene: Dmel\mir-33
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General Information
Symbol
Dmel\mir-33
Species
D. melanogaster
Name
mir-33 stem loop
Annotation Symbol
CR42928
Feature Type
miRNA_gene
FlyBase ID
FBgn0262393
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

dme-mir-33

Key Links
Genomic Location
Cytogenetic map
76C6-76C6
Sequence location
Recombination map
3-46
RefSeq locus
NT_037436 REGION:19797961..19798059
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (2 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in miRNA-mediated post-transcriptional gene silencing
inferred from electronic annotation with Rfam:RF00667
(RNAcentral, 2018-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of RISC complex
inferred from electronic annotation with Rfam:RF00667
(RNAcentral, 2018-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Gene Model and Products
    Number of Transcripts
    3
    Number of Unique Polypeptides
    0

    Please see the JBrowse view of Dmel\mir-33 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Structure
    Experimentally Determined Structures
    Crossreferences
    Comments on Gene Model

    Locations and structures of miRNA models as mapped by miRBase (FBrf0220601).

    Conserved: found throughout the Drosophila genus (FBrf0230987).

    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    mir-33-RA
    FBtr0304280
    NR_048302
    21
    mir-33-RB
    FBtr0472725
    22
    mir-33-RM
    FBtr0304279
    NR_048301
    99
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    miRBase - A searchable database of published miRNA sequences and annotation.
    Sequences Consistent with the Gene Model
    Nucleotide / Polypeptide Records
      Mapped Features

      Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mir-33 using the Feature Mapper tool.

      External Data
      Crossreferences
      Linkouts
      Expression Data
      Testis-specificity index

      The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

      NA

      Transcript Expression
      Additional Descriptive Data
      Marker for
       
      Subcellular Localization
      CV Term
      Polypeptide Expression
      Additional Descriptive Data
      Marker for
       
      Subcellular Localization
      CV Term
      Evidence
      References
      Expression Deduced from Reporters
      High-Throughput Expression Data
      Associated Tools

      JBrowse - Visual display of RNA-Seq signals

      View Dmel\mir-33 in JBrowse
      RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
      Bulk Downloads
      RNA-Seq RPKM values for all genes
      Reference
      See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
      Developmental Proteome: Life Cycle
      Developmental Proteome: Embryogenesis
      External Data and Images
      Linkouts
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      Images
      FlyBase Wiki
      Image Based Resources
      Alleles, Insertions, Transgenic Constructs, and Aberrations
      Classical and Insertion Alleles ( 2 )
      For All Classical and Insertion Alleles Show
       
      Other relevant insertions
      Transgenic Constructs ( 6 )
      For All Alleles Carried on Transgenic Constructs Show
      Transgenic constructs containing/affecting coding region of mir-33
      Transgenic constructs containing regulatory region of mir-33
      Aberrations (Deficiencies and Duplications) ( 0 )
      Inferred from experimentation ( 0 )
      Inferred from location ( 2 )
      Variants
      Variant Molecular Consequences
      Alleles Representing Disease-Implicated Variants
      Phenotypes
      For more details about a specific phenotype click on the relevant allele symbol.
      Lethality
      Allele
      viable, with Scer\GAL4Act5C.PU
      viable, with Scer\GAL4Tub.PU
      Sterility
      Allele
      female fertile
      male fertile
      Phenotype manifest in
      Allele
      Orthologs
      Downloads
      Download All DIOPT Orthologs
      Human Orthologs (via DIOPT v9.1)
      Species\Gene Symbol
      Score
      Best Score
      Best Reverse Score
      Source
      Compara
      Domainoid
      eggNOG
      Hieranoid
      Homologene
      Inparanoid
      OMA
      OrthoDB
      OrthoFinder
      OrthoInspector
      orthoMCL
      Panther
      Phylome
      SonicParanoid
      Alignment
      Complementation?
      Transgene?
      Homo sapiens (Human) (2)
      Hsap\MIR33A
      1 of 14
      Yes
      Yes
      Hsap\MIR33B
      1 of 14
      Yes
      Yes
      Model Organism Orthologs (via DIOPT v9.1)
      Species\Gene Symbol
      Score
      Best Score
      Best Reverse Score
      Source
      Compara
      Domainoid
      eggNOG
      Hieranoid
      Homologene
      Inparanoid
      OMA
      OrthoDB
      OrthoFinder
      OrthoInspector
      orthoMCL
      Panther
      Phylome
      SonicParanoid
      Alignment
      Complementation?
      Transgene?
      Rattus norvegicus (Norway rat) (1)
      Rnor\Mir33
      1 of 14
      Yes
      Yes
      Mus musculus (laboratory mouse) (1)
      Mmus\Mir33
      1 of 14
      Yes
      Yes
      Xenopus tropicalis (Western clawed frog) (0)
      Danio rerio (Zebrafish) (0)
      Caenorhabditis elegans (Nematode, roundworm) (0)
      Anopheles gambiae (African malaria mosquito) (0)
      Arabidopsis thaliana (thale-cress) (0)
      Saccharomyces cerevisiae (Brewer's yeast) (0)
      Schizosaccharomyces pombe (Fission yeast) (0)
      Escherichia coli (enterobacterium) (0)
      Other Organism Orthologs (via OrthoDB)
      Data provided directly from OrthoDB:mir-33. Refer to their site for version information.
      Paralogs
      Downloads
      Download All DIOPT Paralogs
      Paralogs (via DIOPT v9.1)
      Human Disease Associations
      FlyBase Human Disease Model Reports
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Show/hide secondary interactors 
      (data from AllianceMine provided by esyN)
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Other Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      RNA-protein
      Physical Interaction
      Assay
      References
      mir-33 - SmB
      anti tag coimmunoprecipitation, primer specific pcr
      (Xiong et al., 2015)
      mir-33 - SmD1
      anti bait coimmunoprecipitation, primer specific pcr
      (Xiong et al., 2015)
      mir-33 - SmD2
      anti tag coimmunoprecipitation, primer specific pcr
      (Xiong et al., 2015)
      Summary of Genetic Interactions
      esyN Network Diagram
      Other Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Linkouts
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map
      3-46
      Cytogenetic map
      76C6-76C6
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      76C6-76C6
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-46

      (FlyBase, 2016)

      3-43.1

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (5)
      Genomic Clones (11)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (0)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
              Other clones
                RNAi and Array Information
                Linkouts
                Antibody Information
                Laboratory Generated Antibodies
                 
                Commercially Available Antibodies
                 
                Cell Line Information
                Publicly Available Cell Lines
                 
                  Other Stable Cell Lines
                   
                    Other Comments

                    New annotation (CR33569) in release 4.1 of the genome annotation.

                    (FlyBase Genome Annotators, 2005)
                    Relationship to Other Genes
                    Source for database merge of
                    Additional comments

                    The annotation for mir-33 has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. The symbol of the annotation has been changed from CR33569 to CR42928 to reflect this change.

                    (FlyBase Genome Annotators, 2010)
                    Nomenclature History
                    Source for database identify of
                    Nomenclature comments
                    Etymology
                    Synonyms and Secondary IDs (11)
                    Reported As
                    Symbol Synonym
                    33
                    (Han et al., 2011)
                    CR33569
                    (FlyBase Genome Annotators, 2005)
                    CR42928
                    (FlyBase Genome Annotators, 2010)
                    MIR-33
                    (Ylla et al., 2016)
                    dme-miR-33
                    (Volin et al., 2018, Dhahbi et al., 2016, Towler et al., 2015)
                    dme-mir-33
                    (He et al., 2022, miRBase, 2013, Reinhardt et al., 2012, Berezikov et al., 2011, Gesellchen and Boutros, 2004)
                    miR-33
                    (Clerbaux et al., 2021, Kitatani et al., 2020, Toprak et al., 2020, Martin Anduaga et al., 2019, Reichholf et al., 2019, Atilano et al., 2017, Picao-Osorio et al., 2017, Shu et al., 2017, Rainey et al., 2016, Yang et al., 2016, Fulga et al., 2015, Suh et al., 2015, Xiong et al., 2015, Kozomara et al., 2014, Xia and Zhang, 2014, Nesler et al., 2013, Behura et al., 2011, Dávalos et al., 2011, Liu et al., 2011, Ameres et al., 2010, Fernandez-Valverde et al., 2010, Czech et al., 2009, Ibáñez-Ventoso et al., 2008, Stark et al., 2007, Leaman, 2005, Nakahara and Carthew, 2004, Rajewsky and Socci, 2004, Enright et al., 2003, Lai et al., 2003, Stark et al., 2003, Stark et al., 2003)
                    miR-33-5p
                    (Seong et al., 2018)
                    miR33
                    (Zhang et al., 2010)
                    mir-33
                    (Örkenby et al., 2023, Niu et al., 2019, Redmond et al., 2019, Mohammed and Lai, 2016.2.8, Chen et al., 2014, Wang et al., 2014, Daneshvar et al., 2013, Smibert et al., 2013, Bejarano et al., 2012, Schertel et al., 2012, Szuplewski et al., 2012, Cooper et al., 2010, FlyBase Genome Annotators, 2010, Okamura et al., 2009, Liu et al., 2007, Ruby et al., 2007, Burgler and Macdonald, 2005)
                    Name Synonyms
                    mir-33
                    mir-33 stem loop
                    Secondary FlyBase IDs
                    • FBgn0067683
                    • FBgn0262221
                    Datasets (0)
                    Study focus (0)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    Study result (0)
                    Result
                    Result Type
                    Title
                    External Crossreferences and Linkouts ( 14 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    RNAcentral - A comprehensive ncRNA sequence collection representing all ncRNA types from a broad range of organisms
                    Other crossreferences
                    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    MARRVEL_MODEL - MARRVEL (model organism gene)
                    miRBase - A searchable database of published miRNA sequences and annotation.
                    Linkouts
                    DroID - A comprehensive database of gene and protein interactions.
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    References (70)