Gene Dmel\let-7

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General Information

Symbol

Dmel\let-7

Species

D. melanogaster

Name

let-7

Annotation symbol

CR42949

Feature type

miRNA_gene

FlyBase ID

FBgn0262406

Gene Model Status

Current

Stock availability

None publicly available

Also Known As

dme-let-7

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

36F5-36F5

Sequence location

2L:18,472,034..18,472,111 [+]

Genomic Maps

Select View:

modENCODE GBrowse

Summary Information

Automatically generated summary

See sections below for more information

The gene let-7 is referred to in FlyBase by the symbol Dmel\let-7 (CR42949, FBgn0262406). It is a miRNA_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: metamorphosis; regulation of development, heterochronic. 9 alleles are reported. The phenotypes of these alleles are annotated with: wing disc; dopaminergic neuron; wing. It has one annotated transcript. Gene sequence location is 2L:18472034..18472111.

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2012_01

FB2011_10

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36F5-36F5

Limits computationally determined from genome sequence between P{lacW}Aac11^k06710 and P{EP}CG10413^EP2164

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\let-7 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

let-7-RM

FBtr0304323

let-7-RA

FBtr0304324

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

miRBase - the home of microRNA data

•

MI0000416

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

High-Throughput Expression Data

Untitled Document detailed view

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

Expression Clusters

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

locomotor behavior defective | recessive

let-7^Δ.cSa

neuroanatomy defective | recessive

let-7^Δ.cSa

visible, with Scer\GAL4^vg.PM

let-7^Scer\UAS.cCa

Phenotype manifest in

Allele

dopaminergic neuron

let-7^Δ.cSa

wing, with Scer\GAL4^vg.PM

let-7^Scer\UAS.cCa

wing disc, with Scer\GAL4^vg.PM

let-7^Scer\UAS.cCa

wing disc | somatic clone, with Scer\GAL4^Act.PU

let-7^Scer\UAS.cCa

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of let-7	Class	Mutagen	Stocks	Known lesion
let-7^KO.C		recombination FLPase SCEI endonuclease	0	Yes

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of let-7	Mutagen	Known lesion
let-7^+tSa	in vitro construct - genomic fragment	Yes
let-7^cRa	in vitro construct	Yes
let-7^MtnA.PR	in vitro construct - regulatory fusion	Yes
let-7^mut.Scer\UAS	in vitro construct - site directed mutagenesis	Yes
let-7^Scer\UAS.cCa	in vitro construct - regulatory fusion	Yes
let-7^Scer\UAS.cGa	in vitro construct - regulatory fusion	Yes
let-7^Scer\UAS.cSa	in vitro construct - regulatory fusion	Yes
let-7^Δ.cSa	in vitro construct - deletion	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)let-7-C^KO1

(Sokol et al., 2008)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

P{W8,let-7-C}

P{W8,let-7-C^Δlet-7}

P{W8,let-7-C^ΔmiR-100}

P{W8,let-7-C^ΔmiR-125}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )

Terms Based on Experimental Evidence ( 2 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

metamorphosis

inferred from expression pattern

(Sempere et al., 2002)

regulation of development, heterochronic

inferred from mutant phenotype

(Caygill and Johnston, 2008, Sokol et al., 2008)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

regulation of development, heterochronic

traceable author statement

(Banerjee and Slack, 2002)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

miRNA_gene

(Lagos-Quintana et al., 2001, FlyBase Genome Annotators, 2010)

nuclear_gene

(Lagos-Quintana et al., 2001, FlyBase Genome Annotators, 2010)

Interactions & Pathways

Summary of Physical Interactions

Protein-protein

Interacting group

Assay

References

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

let-7 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262406

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dpse\let-7

(Lai et al., 2003)

Linkouts

Stocks & Reagents

Stocks Listed in FlyBase ( 0 )

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 0 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

The annotation for let-7 has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. The symbol of the annotation has been changed from CR32968 to CR42949 to reflect this change.

(FlyBase Genome Annotators, 2010)

The three microRNAs let-7, mir-125 and mir-100 are co-transcribed as a single polycistronic transcription unit (the "let-7-Complex, let-7-C").

(Sokol et al., 2008)

Other Comments

let-7 is required to ensure the appropriate remodeling of the abdominal neuromusculature during the larval-to-adult transition.

(Sokol et al., 2008)

A long primary transcript comprising all the hairpin precursor sequences for mir-100, let-7 and mir-125 has been detected by RT-PCR. Overall orientation not stated: mir-100+ let-7+ mir-125+

(Aravin et al., 2003)

let-7 and mir-125 appear to be expressed from a common precursor RNA.

(Bashirullah et al., 2003)

mir-100, mir-125 and let-7 sequences are clustered within 800bp in 36E5--36F2.

(Sempere et al., 2003)

New annotation (CR32968) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Hormone induced expression of let-7 may control developmental stage transitions.

(Sempere et al., 2002)

A developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 small temporal RNA (stRNA).

(Hutvagner et al., 2001)

External Crossreferences & Linkouts

Sequence Crossreferences

Entrez Gene - A searchable database of RefSeq genes.

•

3772493

Other Crossreferences

miRBase - the home of microRNA data

•

MI0000416

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262406

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0262406

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

modMine - Data generated by the modENCODE project.

•

FBgn0262406

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

CR32968

CR42949

(FlyBase Genome Annotators, 2010, )

dme-let-7

(O'Farrell et al., 2008, Huang and Gu, 2007)

Let-7

(Jing et al., 2005, Yang et al., 2009)

let7

(Neumuller et al., 2008)

Name Synonym

let-7

Secondary FlyBase IDs

FBgn0043885
FBgn0260473
FBgn0262242

References ( 82 )

Generate a list of

All references relating to this gene ( 82 )

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Recent research papers ( 12 )

Ameres et al., 2011, RNA 17(1): 54--63: Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins. [FBrf0212657]
Behura et al., 2011, PLoS ONE 6(7): e21504: Comparative Genomic Analysis of Drosophila melanogaster and Vector Mosquito Developmental Genes. [FBrf0214308]
Cenik et al., 2011, Mol. Cell 42(2): 172--184: Phosphate and R2D2 Restrict the Substrate Specificity of Dicer-2, an ATP-Driven Ribonuclease. [FBrf0213543]
Gerbasi et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(8): 3204--3209: Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis. [FBrf0213185]
Ameres et al., 2010, Science 328(5985): 1534--1539: Target RNA-directed trimming and tailing of small silencing RNAs. [FBrf0211070]
Fernandez-Valverde et al., 2010, RNA 16(10): 1881--1888: Dynamic isomiR regulation in Drosophila development. [FBrf0211828]
Garbuzov and Tatar, 2010, Fly 4(4): 306--311: Hormonal regulation of Drosophila microRNA let-7 and miR-125 that target innate immunity. [FBrf0212526]
Gehrke et al., 2010, Nature 466(7306): 637--641: Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. [FBrf0211387]
Gerbasi et al., 2010, Mol. Cell. Proteomics 9(9): 1866--1872: Proteomics Identification of Drosophila Small Interfering RNA-associated Factors. [FBrf0211707]
Iwasaki et al., 2010, Mol. Cell 39(2): 292--299: Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. [FBrf0214214]
Miyoshi et al., 2010, RNA 16(3): 506--515: Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in Drosophila. [FBrf0210024]
Moretti et al., 2010, RNA 16(12): 2493--2502: Mechanism of translational regulation by miR-2 from sites in the 5' untranslated region or the open reading frame. [FBrf0212369]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2010

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