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Gene Dmel\mir-277

General Information
Symbol	Dmel\mir-277	Species	D. melanogaster
Name	mir-277 stem loop	Annotation symbol	CR42968
Feature type	miRNA_gene	FlyBase ID	FBgn0262419
Gene Model Status	Current	Stock availability	None publicly available
Also Known As	dme-mir-277, miR277
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	85F10-85F10	Sequence location	3R:5,925,744..5,925,843 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene mir-277 stem loop is referred to in FlyBase by the symbol Dmel\mir-277 (CR42968, FBgn0262419). It is a miRNA_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: positive regulation of neuron death. 7 alleles are reported. The phenotypes of these alleles are annotated with: wing margin; eye; wing. It has one annotated transcript. Gene sequence location is 3R:5925744..5925843.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 85F10-85F10
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mir-277 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Locations and structures of miRNA models as mapped by miRBase (FBrf0220601).
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt)   mir-277-RM FBtr0304361  NR_048360   100   mir-277-RA FBtr0304362  NR_048361   23
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences	miRBase - the home of microRNA data • MI0000360
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence   Name
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4da.G32 mir-277Scer\UAS.T:Disc\RFP partially lethal - majority die | pupal stage, with Scer\GAL4sd-SG29.1 mir-277Scer\UAS.T:Ppyr\LUC viable, with Fmr1+tLa, mir-34null mir-277+tLa viable, with Scer\GAL4Act5C.PU mir-277Scer\UAS.cSa Other Phenotypes Allele gravitaxis defective | conditional, with Fmr1+tLa, mir-34null mir-277+tLa heat stress response defective | conditional, with Fmr1+tLa, mir-34null mir-277+tLa neuroanatomy defective | adult stage, with Scer\GAL4GMR.PF mir-277Scer\UAS.cTa neuroanatomy defective | conditional, with Fmr1+tLa, mir-34null mir-277+tLa short lived, with Fmr1+tLa, mir-34null mir-277+tLa visible, with Scer\GAL4Bx-MS1096 mir-277Scer\UAS.T:Disc\RFP visible, with Scer\GAL4sd-SG29.1 mir-277Scer\UAS.T:Ppyr\LUC visible | adult stage, with Scer\GAL4GMR.PF mir-277Scer\UAS.cTa Phenotype manifest in Allele adult brain | conditional, with Fmr1+tLa, mir-34null mir-277+tLa eye, with Scer\GAL4GMR.PF mir-277Scer\UAS.cTa wing, with Scer\GAL4Bx-MS1096 mir-277Scer\UAS.T:Disc\RFP wing, with Scer\GAL4sd-SG29.1 mir-277Scer\UAS.T:Ppyr\LUC wing margin, with Scer\GAL4sd-SG29.1 mir-277Scer\UAS.T:Ppyr\LUC
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of mir-277 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mir-277 Class Mutagen Stocks Known lesion mir-277+tLa in vitro construct - genomic fragment 0 Yes mir-277pri.Ubi in vitro construct - regulatory fusion 0 Yes mir-277Scer\UAS.cluster.cLa in vitro construct - regulatory fusion 0 Yes mir-277Scer\UAS.cSa in vitro construct - regulatory fusion 0 Yes mir-277Scer\UAS.cTa in vitro construct - regulatory fusion 0 Yes mir-277Scer\UAS.T:Disc\RFP in vitro construct 0 Yes mir-277Scer\UAS.T:Ppyr\LUC in vitro construct 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)mir-34 (Liu et al., 2012)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct M{UAS-mir-277.S} NA P{UAS-DsRed-mir-277.L} NA P{UAS-LUC-mir-277.T} NA P{UAS-mir-34+mir-277.L} NA P{UAS-mir-277.T} NA characterization construct P{mir-277-Fmr1+tLa} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 1 unique term )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
positive regulation of neuron death inferred from genetic interaction with Fmr1 (Tan et al., 2012)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	miRNA_gene (Stark et al., 2003, Stark et al., 2003, Stark et al., 2003, FlyBase Genome Annotators, 2010) nuclear_gene (Stark et al., 2003, Stark et al., 2003, Stark et al., 2003, FlyBase Genome Annotators, 2010)
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mir-277 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262419
Orthologs
OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search No orthologs identified Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (None identified)
No orthologies identified
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 0 )
Genomic Clones ( 1 )
	BACR39F04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 0 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 12798086
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	The annotation for mir-277 has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. The symbol of the annotation has been changed from CR33588 to CR42968 to reflect this change. (FlyBase Genome Annotators, 2010)
Other Comments
	New annotation (CR33588) in release 4.1 of the genome annotation. (FlyBase Genome Annotators, 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NR_048360 NR_048361 Entrez Gene - A searchable database of RefSeq genes. • 12798086
Other Crossreferences
	miRBase - the home of microRNA data • MI0000360
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262419 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0262419 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 12798086 modMine - Data generated by the modENCODE project. • FBgn0262419
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CR33588 (FlyBase Genome Annotators, 2005) CR42968 (FlyBase Genome Annotators, 2010, ) dme-mir-277 (Berezikov et al., 2011) Dme-miR-277 (Zhou et al., 2009) mir-277 (Daneshvar et al., 2013, Qian et al., 2011, Schertel et al., 2012, FlyBase Genome Annotators, 2010, Luhur and Kumar, 2008, Neumuller et al., 2008, Liu et al., 2007, Park et al., 2007, Okamura et al., 2009, Ruby et al., 2007, Burgler and Macdonald, 2005, Bejarano et al., 2012, Bejarano et al., 2012, Liu et al., 2012) miR-277 (Bejarano et al., 2012, Yu et al., 2011, Liu et al., 2011, Ameres et al., 2010, Leaman, 2005, Forstemann et al., 2005, Gesellchen and Boutros, 2004, Lai et al., 2003, Bartel, 2004, Stark et al., 2003, Stark et al., 2003, Stark et al., 2003, Ghildiyal et al., 2006, Behura, 2007, Förstemann et al., 2007, Horwich et al., 2007, Tadros et al., 2007, Kadener et al., 2009, Sabin et al., 2009, Rehmsmeier et al., 2004, Behm-Ansmant et al., 2006, Lee et al., 2009, Czech et al., 2009, Silver et al., 2007, Enright et al., 2003, Horwich and Zamore, 2008, Ibáñez-Ventoso et al., 2008, Helfer et al., 2012, Liu et al., 2012, Tan et al., 2012, Fukunaga et al., 2012) miR277 (Jiang et al., 2005, Zhang et al., 2010) MiR-277 (Yang et al., 2009)
Name Synonym	mir-277   miR-277 (Rehmsmeier et al., 2004) mir-277 stem loop
Secondary FlyBase IDs
	FBgn0067715 FBgn0262261
References ( 53 )
Generate a list of	All references relating to this gene ( 53 )
List References by type	Research paper  ( 40 ) Supplementary material  ( 5 ) Review  ( 4 ) Abstract  ( 2 ) FlyBase analysis  ( 2 )
Recent research papers ( 12 )
	Daneshvar et al., 2013, Biol. Open 2(1): 1--9 MicroRNA miR-308 regulates dMyc through a negative feedback loop in Drosophila. [FBrf0220584] Bejarano et al., 2012, Development 139(15): 2821--2831 A genome-wide transgenic resource for conditional expression of Drosophila microRNAs. [FBrf0218840] Fukunaga et al., 2012, Cell 151(3): 533--546 Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals. [FBrf0219804] Helfer et al., 2012, PLoS ONE 7(1): e28907 AU-Rich Element-Mediated mRNA Decay Can Occur Independently of the miRNA Machinery in Mouse Embryonic Fibroblasts and Drosophila S2-Cells. [FBrf0217194] Liu et al., 2012, Nature 482(7386): 519--523 The microRNA miR-34 modulates ageing and neurodegeneration in Drosophila. [FBrf0217556] Marrone et al., 2012, BMC Cell Biol. 13: 26 Dg-Dys-Syn1 signaling in Drosophila regulates the microRNA profile. [FBrf0220581] Schertel et al., 2012, Genetics 192(4): 1543--1552 Functional Characterization of Drosophila microRNAs by a Novel in Vivo Library. [FBrf0220194] Tan et al., 2012, PLoS Genet. 8(5): e1002681 MicroRNA-277 Modulates the Neurodegeneration Caused by Fragile X Premutation rCGG Repeats. [FBrf0218227] Berezikov et al., 2011, Genome Res. 21(2): 203--215 Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergence. [FBrf0213080] Liu et al., 2011, Curr. Biol. 21(22): 1888--1893 The Exoribonuclease Nibbler Controls 3' End Processing of MicroRNAs in Drosophila. [FBrf0216782] Qian et al., 2011, Genomics 97(5): 294--303 The full-length transcripts and promoter analysis of intergenic microRNAs in Drosophila melanogaster. [FBrf0213652] Yu et al., 2011, PLoS Genet. 7(3): e1001340 Triplet Repeat-Derived siRNAs Enhance RNA-Mediated Toxicity in a Drosophila Model for Myotonic Dystrophy. [FBrf0213314] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...