FB2022_02, released March 29, 2022

Gene: Dmel\mir-277

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General Information
Symbol
Dmel\mir-277
Species
D. melanogaster
Name
mir-277 stem loop
Annotation Symbol
CR42968
Feature Type
miRNA_gene
FlyBase ID
FBgn0262419
Gene Model Status
Current
Stock Availability
2 publicly available
Gene Summary
microRNA that carries out post-transcriptional gene regulation, modulates rCGG repeat-mediated neurodegeneration and the pathogenesis of FXTAS neurodegenerative disorder by post-transcriptionally regulating the expression of specific mRNAs involved in the disease (Interactive Fly, mir-277 )
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

dme-miR-277, miR277

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-49
RefSeq locus
NT_033777 REGION:10100022..10100121
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables mRNA 3'-UTR binding
inferred from direct assay
(Cerro-Herreros et al., 2016, Tan et al., 2012)
enables mRNA binding involved in posttranscriptional gene silencing
inferred from direct assay
(Cerro-Herreros et al., 2016, Tan et al., 2012)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in gene silencing by miRNA
inferred from direct assay
(Cerro-Herreros et al., 2016, Tan et al., 2012)
involved_in miRNA-mediated gene silencing by inhibition of translation
inferred from direct assay
(Tan et al., 2012)
involved_in negative regulation of muscle organ development
inferred from mutant phenotype
(Cerro-Herreros et al., 2016)
involved_in positive regulation of neuron death
inferred from mutant phenotype
(Tan et al., 2012)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Summary (Interactive Fly)

    microRNA that carries out post-transcriptional gene regulation, modulates rCGG repeat-mediated neurodegeneration and the pathogenesis of FXTAS neurodegenerative disorder by post-transcriptionally regulating the expression of specific mRNAs involved in the disease

    Gene Model and Products
    Number of Transcripts
    3
    Number of Unique Polypeptides
    0

    Please see the JBrowse view of Dmel\mir-277 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Structure New Section
    Comments on Gene Model

    Locations and structures of miRNA models as mapped by miRBase (FBrf0220601).

    Conserved: found throughout the Drosophila genus (FBrf0230987).

    Sequence Ontology: Class of Gene
    miRNA_gene
    (FlyBase Genome Annotators, 2010, Stark et al., 2003, Stark et al., 2003, Stark et al., 2003)
    nuclear_gene
    (FlyBase Genome Annotators, 2010, Stark et al., 2003, Stark et al., 2003, Stark et al., 2003)
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    mir-277-RA
    FBtr0304362
    NR_048361
    23
    mir-277-RB
    FBtr0472721
    21
    mir-277-RM
    FBtr0304361
    NR_048360
    100
    Additional Transcript Data and Comments
    Reported size (kB)
    Comments
    External Data
    Crossreferences
    miRBase - A searchable database of published miRNA sequences and annotation.
    Sequences Consistent with the Gene Model
    Nucleotide / Polypeptide Records
      Mapped Features

      Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mir-277 using the Feature Mapper tool.

      External Data
      Crossreferences
      Linkouts
      Expression Data
      Expression Summary Ribbons
      Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
      For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
      Transcript Expression
      Additional Descriptive Data
      Marker for
       
      Subcellular Localization
      CV Term
      Polypeptide Expression
      Additional Descriptive Data
      Marker for
       
      Subcellular Localization
      CV Term
      Evidence
      References
      Expression Deduced from Reporters
      High-Throughput Expression Data
      Associated Tools

      GBrowse - Visual display of RNA-Seq signals

      View Dmel\mir-277 in GBrowse 2
      RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
      Bulk Downloads
      RNA-Seq RPKM values for all genes
      Reference
      See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
      Developmental Proteome: Life Cycle
      Developmental Proteome: Embryogenesis
      External Data and Images
      Linkouts
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      Images
      FlyBase Wiki
      Image Based Resources
      Alleles, Insertions, Transgenic Constructs, and Aberrations
      Classical and Insertion Alleles ( 0 )
      For All Classical and Insertion Alleles Show
       
      Other relevant insertions
      Transgenic Constructs ( 13 )
      For All Alleles Carried on Transgenic Constructs Show
      Transgenic constructs containing/affecting coding region of mir-277
      Transgenic constructs containing regulatory region of mir-277
      Aberrations (Deficiencies and Duplications) ( 2 )
      Inferred from experimentation ( 2 )
      Gene disrupted in
      Df(3R)mir-34
      (Liu et al., 2012)
      Df(3R)mir-277-34-KO
      (Chen et al., 2014)
      Inferred from location ( 0 )
      Alleles Representing Disease-Implicated Variants
      Phenotypes
      For more details about a specific phenotype click on the relevant allele symbol.
      Lethality
      Allele
      lethal, with Scer\GAL4da.G32
      partially lethal - majority die, with Scer\GAL4sd-SG29.1
      viable, with Fmr1+tLa, mir-34null
      viable, with Scer\GAL4Act5C.PU
      viable, with Scer\GAL4Tub.PU
      viable | male, with Scer\GAL4Act5C.PI
      viable | male, with Scer\GAL4da.G32
      Other Phenotypes
      Allele
      abnormal flight, with Scer\GAL4Mhc.PW
      abnormal heat stress response | conditional, with Fmr1+tLa, mir-34null
      abnormal locomotor behavior | adult stage, with Scer\GAL4Mhc.PW
      abnormal locomotor behavior | adult stage | conditional, with Fmr1+tLa, mir-34null
      abnormal neuroanatomy | adult stage, with Scer\GAL4GMR.PF
      abnormal neuroanatomy | conditional, with Fmr1+tLa, mir-34null
      abnormal size | adult stage, with Scer\GAL4Mhc.PW
      short lived
      short lived, with Fmr1+tLa, mir-34null
      short lived | nutrition conditional
      short lived | nutrition conditional, with Scer\GAL4Mhc.PW
      some die during pupal stage, with Scer\GAL4sd-SG29.1
      visible, with Scer\GAL4Bx-MS1096
      visible, with Scer\GAL4sd-SG29.1
      visible | adult stage, with Scer\GAL4GMR.PF
      Phenotype manifest in
      Allele
      adult brain | conditional, with Fmr1+tLa, mir-34null
      eye, with Scer\GAL4GMR.PF
      indirect flight muscle cell | adult stage, with Scer\GAL4Mhc.PW
      wing, with Scer\GAL4Bx-MS1096
      wing, with Scer\GAL4sd-SG29.1
      wing margin, with Scer\GAL4sd-SG29.1
      Orthologs
      Downloads
      Download All DIOPT Orthologs
      Human Orthologs (via DIOPT v8.0)
      Homo sapiens (Human) (0)
      No records found.
      Model Organism Orthologs (via DIOPT v8.0)
      Mus musculus (laboratory mouse) (0)
      No records found.
      Rattus norvegicus (Norway rat) (0)
      No records found.
      Xenopus tropicalis (Western clawed frog) (0)
      No records found.
      Danio rerio (Zebrafish) (0)
      No records found.
      Caenorhabditis elegans (Nematode, roundworm) (0)
      No records found.
      Arabidopsis thaliana (thale-cress) (0)
      No records found.
      Saccharomyces cerevisiae (Brewer's yeast) (0)
      No records found.
      Schizosaccharomyces pombe (Fission yeast) (0)
      No records found.
      Other Organism Orthologs (via OrthoDB)
      Paralogs
      Downloads
      Download All DIOPT Paralogs
      Paralogs (via DIOPT v8.0)
      Drosophila melanogaster (Fruit fly) (0)
      No records found.
      Human Disease Associations
      FlyBase Human Disease Model Reports
        Disease Model Summary Ribbon
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Allele
        Disease
        Evidence
        References
        Potential Models Based on Orthology ( 0 )
        Human Ortholog
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 2 )
        Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
        Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
        Homo sapiens (Human)
        Gene name
        Score
        OMIM
        OMIM Phenotype
        DO term
        Complementation?
        Transgene?
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Fmr1|mir-277|mir-34
        suppressible
        Eip74EF
        (Liu et al., 2012)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-49
        Cytogenetic map
        85F10-85F10
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        85F10-85F10
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-49

        (FlyBase, 2016)

        3-46.6

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (2)
        Genomic Clones (19)
        cDNA Clones (0)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
          Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
                Other clones
                  RNAi and Array Information
                  Linkouts
                  GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                  Antibody Information
                  Laboratory Generated Antibodies
                   
                  Commercially Available Antibodies
                   
                  Other Information
                  Relationship to Other Genes
                  Source for database identify of
                  Source for database merge of
                  Additional comments

                  The annotation for mir-277 has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. The symbol of the annotation has been changed from CR33588 to CR42968 to reflect this change.

                  (FlyBase Genome Annotators, 2010)
                  Other Comments

                  New annotation (CR33588) in release 4.1 of the genome annotation.

                  (FlyBase Genome Annotators, 2005)
                  Origin and Etymology
                  Discoverer
                  Etymology
                  Identification
                  External Crossreferences and Linkouts ( 17 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  RNAcentral - A comprehensive ncRNA sequence collection representing all ncRNA types from a broad range of organisms
                  Other crossreferences
                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                  FlyMine - An integrated database for Drosophila genomics
                  GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MARRVEL_MODEL - MARRVEL (model organism gene)
                  miRBase - A searchable database of published miRNA sequences and annotation.
                  modMine - A data warehouse for the modENCODE project
                  Linkouts
                  DroID - A comprehensive database of gene and protein interactions.
                  Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                  Synonyms and Secondary IDs (12)
                  Reported As
                  Symbol Synonym
                  CR33588
                  (FlyBase Genome Annotators, 2005)
                  CR42968
                  (FlyBase Genome Annotators, 2010)
                  Dme-miR-277
                  (Zhou et al., 2009)
                  MIR-277
                  (Ylla et al., 2016)
                  MiR-277
                  (Yang et al., 2009)
                  dme-miR-277
                  (Wang et al., 2019, Volin et al., 2018, Dhahbi et al., 2016, Towler et al., 2015)
                  dme-miR-277-5p
                  (Chandra et al., 2015)
                  dme-mir-277
                  (Mohammed et al., 2014, miRBase, 2013, Berezikov et al., 2011)
                  miR-277
                  (Jin et al., 2020, Kinser and Pincus, 2020, Kitatani et al., 2020, Li et al., 2020, McNeill et al., 2020, Shi et al., 2020, Toprak et al., 2020, Xia et al., 2020, Reichholf et al., 2019, Alberti et al., 2018, Ueda et al., 2018, Atilano et al., 2017, Chandra et al., 2017, Church et al., 2017, Picao-Osorio et al., 2017, Shu et al., 2017, Rainey et al., 2016, Fulga et al., 2015, Suh et al., 2015, Kong et al., 2014, Esslinger et al., 2013, Lucas and Raikhel, 2013, Mezan et al., 2013, Nesler et al., 2013, Bejarano et al., 2012, Fukunaga et al., 2012, Helfer et al., 2012, Liu et al., 2012, Ren et al., 2012, Tan et al., 2012, Liu et al., 2011, Yoon et al., 2011, Yu et al., 2011, Ameres et al., 2010, Czech et al., 2009, Kadener et al., 2009, Lee et al., 2009, Sabin et al., 2009, Horwich and Zamore, 2008, Ibáñez-Ventoso et al., 2008, Behura, 2007, Förstemann et al., 2007, Horwich et al., 2007, Silver et al., 2007, Tadros et al., 2007, Behm-Ansmant et al., 2006, Ghildiyal et al., 2006, Forstemann et al., 2005, Leaman, 2005, Bartel, 2004, Gesellchen and Boutros, 2004, Rehmsmeier et al., 2004, Enright et al., 2003, Lai et al., 2003, Stark et al., 2003, Stark et al., 2003, Stark et al., 2003)
                  miR277
                  (Nozawa et al., 2016, Zhang et al., 2010, Jiang et al., 2005)
                  mir-277
                  (Bejarano and Lai, 2021, Bejarano et al., 2021, Hanyu-Nakamura et al., 2019, Mohammed and Lai, 2016.2.8, Chen et al., 2014, Wang et al., 2014, Yang et al., 2014, Yeh et al., 2014, Daneshvar et al., 2013, Bejarano et al., 2012, Bejarano et al., 2012, Liu et al., 2012, Schertel et al., 2012, Szuplewski et al., 2012, Qian et al., 2011, FlyBase Genome Annotators, 2010, Okamura et al., 2009, Luhur and Kumar, 2008, Neumuller et al., 2008, Liu et al., 2007, Park et al., 2007, Ruby et al., 2007, Burgler and Macdonald, 2005)
                  Name Synonyms
                  miR-277
                  (Westholm et al., 2014, Rehmsmeier et al., 2004)
                  mir-277
                  mir-277 stem loop
                  Secondary FlyBase IDs
                  • FBgn0067715
                  • FBgn0262261
                  Datasets (0)
                  Study focus (0)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  Study result (0)
                  Result
                  Result Type
                  Title
                  References (112)