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Gene Dmel\mir-14

General Information
Symbol	Dmel\mir-14	Species	D. melanogaster
Name	mir-14 stem loop	Annotation symbol	CR43013
Feature type	miRNA_gene	FlyBase ID	FBgn0262447
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	dme-mir-14, mir14, l(2)k10213, CR33010
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	45F1-45F1	Sequence location	2R:5,441,267..5,441,329 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene mir-14 stem loop is referred to in FlyBase by the symbol Dmel\mir-14 (CR43013, FBgn0262447). It is a miRNA_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: response to starvation; negative regulation of programmed cell death; regulation of lipid metabolic process; wing disc dorsal/ventral pattern formation; insulin-like growth factor receptor signaling pathway. 18 alleles are reported. The phenotypes of these alleles are annotated with: adult adipose system; wing; anterior pupal spiracle. It has one annotated transcript. Gene sequence location is 2R:5441267..5441329.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	References Thomsen et al., 2010, Genome Biol. 11(9): R93 Sequence features BKN40473
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 45F1-45F1   Limits computationally determined from genome sequence between P{EP}EP2165&P{lacW}l(2)k01301k01301 and P{lacW}l(2)k09501k09501
Experimentally Determined Cytological Location
Cytogenetic map Notes References 45F1-45F2   (determined by in situ hybridisation)   (Spradling et al., 1999) 45F1-45F2   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mir-14 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt)   mir-14-RM FBtr0304458     63   mir-14-RA FBtr0304459     21
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences	miRBase - the home of microRNA data • MI0000136
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence   Name AJ421776   AQ025929
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Sempere et al., 2003)
Additional Descriptive Data
	Northern analysis of 24 microRNAs showed that a number of them were expressed constituitively throughout development. (Sempere et al., 2003)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | partially mir-14Δ1 lethal | recessive mir-14k10213 semi-lethal | pupal stage mir-14Δ1 Other Phenotypes Allele osmotic stress response defective mir-14Δ1 short lived mir-14Δ1 short lived | female mir-14Δ1 starvation stress response defective | adult stage mir-14Δ1 visible, with Scer\GAL4ap-md544 mir-14Scer\UAS.T:Disc\RFP-DsRed2 Phenotype manifest in Allele adult adipose system mir-14Δ1 anterior pupal spiracle mir-14Δ1 wing, with Scer\GAL4ap-md544 mir-14Scer\UAS.T:Disc\RFP-DsRed2
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of mir-14 Class Mutagen Stocks Known lesion mir-14k10213 P-element activity 2 -- mir-14Δ1 1 Yes mir-14EP2-1142 0 -- mir-14EP2-235 0 -- mir-14EP2-356 0 -- mir-14EP2-402 0 -- mir-14EP2-814 0 -- mir-14F393.1 Delta2-3 0 Yes mir-14GS2072 0 Yes mir-14GS7322 0 Yes mir-14GS9193 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mir-14 Class Mutagen Stocks Known lesion mir-14+t3.4 in vitro construct - genomic fragment 0 Yes mir-14GMR.PX in vitro construct - regulatory fusion 0 Yes mir-14Scer\UAS.cUa in vitro construct - regulatory fusion 0 Yes mir-14Scer\UAS.T:Disc\RFP-DsRed2 in vitro construct - regulatory fusion 0 Yes mir-14Scer\UAS.T:Disc\RFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mir-14tub.PV in vitro construct - regulatory fusion 0 Yes mir-14αTub84B.PS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UAS-dsRed2-mir-14.B} NA P{UAS-DsRed-mir-14} NA P{UAS-mir-14.U} NA characterization construct P{GMR-mir-14.X} NA P{mir-14+t3.4} NA P{tub-mir-14.V} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}mir-14k10213 No miscellaneous insertions PBac{Dmw}F1-2 NA insertion of mobile activating element P{EP}mir-14EP2-235 NA P{EP}mir-14EP2-356 NA P{EP}mir-14EP2-402 NA P{EP}mir-14EP2-814 NA P{EP}mir-14EP2-1142 NA P{GSV1}EP-65EP-65 NA P{GSV1}mir-14GS2072 NA P{GSV2}mir-14GS7322 NA P{GSV6}mir-14GS9193 NA P{Mae-UAS.6.11}mir-14F393.1 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
insulin-like growth factor receptor signaling pathway inferred from mutant phenotype (Varghese et al., 2010) negative regulation of programmed cell death inferred from genetic interaction with Nc AND inferred from genetic interaction with W AND inferred from genetic interaction with grim AND inferred from genetic interaction with rpr AND inferred from mutant phenotype (Xu et al., 2003) regulation of lipid metabolic process inferred from mutant phenotype (Xu et al., 2003) response to starvation inferred from mutant phenotype (Varghese et al., 2010) wing disc dorsal/ventral pattern formation inferred from genetic interaction with Bx, ap (Bejarano et al., 2008)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
anti-apoptosis non-traceable author statement (Ambros, 2003) traceable author statement (Nelson et al., 2003, He and Hannon, 2004, Bergmann and Lane, 2003, Xu et al., 2004) regulation of lipid metabolic process traceable author statement (Ambros, 2003) response to stress non-traceable author statement (Ambros, 2003)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	miRNA_gene (Lagos-Quintana et al., 2001, FlyBase Genome Annotators, 2010) nuclear_gene (Lagos-Quintana et al., 2001, FlyBase Genome Annotators, 2010)
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mir-14 allele Gene References mir-14+t3.4 rpr (Xu et al., 2003) mir-14GMR.PX rpr (Xu et al., 2003) mir-14k10213 rpr (Xu et al., 2003) mir-14Δ1 rpr (Xu et al., 2003) unspecified os (Mukherjee et al., 2006) pbl (Gregory et al., 2007)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262447
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dpse\mir-14
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	33067 w*; mir-14Δ1/CyO 10982 w*; P{lacW}mir-14k10213/CyO, P{sevRas1.V12}FK1
Kyoto	111283 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}mir-14k10213 /CyO y+
Genomic Clones ( 2 )
	BACR14J24 BACR48G21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 0 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 170868
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	The annotation for mir-14 has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. The symbol of the annotation has been changed from CR33010 to CR43013 to reflect this change. (FlyBase Genome Annotators, 2010)
Other Comments
	mir-14 may be a dose dependant regulator of triacylglycerol and diacylglycerol and suppresses cell death. (Xu et al., 2003) New annotation (CR33010) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	DDBJ / EMBL / GenBank • AJ421776 AQ025929 Entrez Gene - A searchable database of RefSeq genes. • 3772293
Other Crossreferences
	miRBase - the home of microRNA data • MI0000136
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262447 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0262447 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 170868 modMine - Data generated by the modENCODE project. • FBgn0262447
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	CR33010 (Quinones-Coello, 2007, Molnar et al., 2006) CR43013 (FlyBase Genome Annotators, 2010, ) dme-mir-14 (Gesellchen and Boutros, 2004, Huang and Gu, 2007) dme-miR-14 (O'Farrell et al., 2008) l(2)k10213 (Moberg and Hariharan, 2003, Spradling et al., 1999, ) mir-14 (FlyBase Genome Annotators, 2010, Mukherjee et al., 2006, Quinones-Coello, 2007, Molnar et al., 2006, Bejarano et al., 2008, Neumuller et al., 2008, Link et al., 2007, Liu et al., 2007, Martin et al., 2009, Okamura et al., 2009, Ruby et al., 2007) miR-14 (Leaman, 2005, Alvarez-Garcia and Miska, 2005, Miska, 2005, Wienholds, 2005, Lai et al., 2003, He and Hannon, 2004, Bartel, 2004, Nelson et al., 2003, Sempere et al., 2003, Lee et al., 2001, Lagos-Quintana et al., 2001, Stark et al., 2003, Stark et al., 2003, Stark et al., 2005, Varghese and Cohen, 2007, Rehwinkel et al., 2006, Kadener et al., 2009, Kadener et al., 2009, Czech et al., 2009, Ghildiyal et al., 2010, Varghese et al., 2010, Gehrke et al., 2010, Seitz et al., 2011, Behura et al., 2011, Thomsen et al., 2010) Mir-14 (Vidal and Cagan, 2006, Danial and Korsmeyer, 2004) mir14 (Gregory et al., 2007, Kadener et al., 2009) miR14 (Berdnik et al., 2008)
Name Synonym	mir-14   mir-14 stem loop
Secondary FlyBase IDs
	FBgn0021957 FBgn0046827 FBgn0262306
References ( 61 )
Generate a list of	All references relating to this gene ( 61 )
List References by type	Research paper  ( 32 ) Supplementary material  ( 6 ) Review  ( 19 ) FlyBase analysis  ( 3 ) Computer file  ( 1 )
Recent research papers ( 6 )
	Behura et al., 2011, PLoS ONE 6(7): e21504 Comparative Genomic Analysis of Drosophila melanogaster and Vector Mosquito Developmental Genes. [FBrf0214308] Seitz et al., 2011, Silence 2: 4 A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation. [FBrf0214042] Gehrke et al., 2010, Nature 466(7306): 637--641 Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. [FBrf0211387] Ghildiyal et al., 2010, RNA 16(1): 43--56 Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway. [FBrf0209572] Thomsen et al., 2010, Genome Biol. 11(9): R93 Genome-wide analysis of mRNA decay patterns during early Drosophila development. [FBrf0212195] Varghese et al., 2010, Genes Dev. 24(24): 2748--2753 Drosophila miR-14 regulates insulin production and metabolism through its target, sugarbabe. [FBrf0212621] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...