Gene Dmel\ban

Comments

External Data

Crossreferences

miRBase - the home of microRNA data

•

MI0000387

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

larval stage

wing disc

brain | restricted

Additional Descriptive Data

The ban microRNA is detected during all stages of development by northern analysis although level of expression varies with developmental stage. Expression is highest in 12-24 h embryos and 1st instar larvae. An assay employed a ubiquitously expressed GFP sensor protein whose transcript contained ban target sites and caused silencing of the reporter in the regions where ban microRNA is expressed. This assay indicates that ban microRNA is expressed throughout the wing disc but has the highest expression in areas of proliferation. A similar correlation between ban microRNA expression and cell proliferation was observed in the larval brain.

Marker for

Subcellular Localization

CV Term

Notes

High-Throughput Expression Data

Untitled Document detailed view

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

Expression Clusters

External Data & Images

(Hipfner et al., 2002, Bilen et al., 2006)

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^ey.PH

ban^LAS55

lethal | P-stage, with Scer\GAL4^Act5C.PI

ban^LAS55

lethal | pupal stage

ban¹²

lethal | pupal stage, with Df(3L)ban^Δ1, Scer\GAL4^unspecified

lethal | pupal stage (with Df(3L)emc-E12)

ban¹²

viable

ban²⁰

viable, with Scer\GAL4[-]:

viable (with Df(3L)ban^Δ1)

ban^L1170a

viable (with Df(3L)ban^Δ1), with Scer\GAL4[-]:

viable (with Df(3L)emc-E12)

ban²⁰

Sterility

Allele

female sterile (with Df(3L)ban^Δ1)

ban^L1170a

female sterile (with Df(3L)ban^Δ1), with Scer\GAL4[-]:

ban^EP3208

female sterile | partially, with Scer\GAL4[-]:

female sterile | partially (with Df(3L)ban^Δ1), with Scer\GAL4[-]:

sterile

ban²⁰

sterile (with Df(3L)emc-E12)

ban²⁰

Other Phenotypes

Allele

decreased cell death, with Scer\GAL4^GMR.PF

ban^EP3622

decreased cell number, with Scer\GAL4[-]:

decreased cell number (with ban^EP3622), with Scer\GAL4[-]:

ban^UY3207

decreased cell number (with ban^UY3207), with Scer\GAL4[-]:

ban^EP3622

decreased cell number (with Df(3L)ban^Δ1)

ban^L1170a

decreased cell number (with Df(3L)ban^Δ1), with Scer\GAL4[-]:

decreased cell number | cell non-autonomous, with Scer\GAL4^en-e16E

ban^EP3622

decreased cell size, with Scer\GAL4^ap-md544

ban^EP3622

decreased cell size, with Scer\GAL4^en-e16E

ban^EP3622

increased cell death

ban^EP3622

increased cell death (with ban^EP3622)

ban^L1170a

increased cell death (with ban^L1170a)

ban^EP3622

increased cell number | cell autonomous, with Scer\GAL4^en-e16E

ban^EP3622

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

ban^EP3622

locomotor rhythm defective, with Scer\GAL4^P2.4.Pdf

ban^EP3622

locomotor rhythm defective, with Scer\GAL4^tim.PE

ban^EP3622

neuroanatomy defective | larval stage, with Scer\GAL4^arm.PS

ban^Scer\UAS.cPa

neuroanatomy defective | larval stage, with Scer\GAL4^C855a

ban^{D.Scer\UAS.T:Avic\GFP-EGFP}

neuroanatomy defective | larval stage, with Scer\GAL4^elav-C155

ban^EP3208

size defective, with Scer\GAL4^GMR.PF

small body, with Scer\GAL4[-]:

small body (with ban^EP3622), with Scer\GAL4[-]:

ban^UY3207

small body (with ban^UY3207), with Scer\GAL4[-]:

ban^EP3622

small body (with Df(3L)ban^Δ1)

ban^L1170a

small body (with Df(3L)ban^Δ1), with Scer\GAL4[-]:

small body (with Df(3L)emc), with Scer\GAL4[-]:

visible, with Scer\GAL4^ap-md544

ban^EP3622

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^en-e16E

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^sca-537.4

ban^EP3208

Phenotype manifest in

Allele

anterior fascicle & synapse, with Scer\GAL4^elav-C155

ban^EP3208

cell cycle, with Scer\GAL4^ptc-559.1

ban^{C.Scer\UAS.T:Avic\GFP-EGFP}

dendrite | larval stage, with Scer\GAL4^arm.PS

ban^Scer\UAS.cPa

embryonic/larval optic lobe | larval stage, with Scer\GAL4^C855a

ban^{D.Scer\UAS.T:Avic\GFP-EGFP}

eye, with Scer\GAL4^GMR.PF

fusome | larval stage (with Df(3L)emc-E12)

ban¹²

germline cell | larval stage

ban¹²

germline cell | larval stage (with Df(3L)emc-E12)

ban¹²

germline stem cell (with Df(3L)emc-E12)

ban²⁰

interommatidial bristle | ectopic, with Scer\GAL4^GMR.PF

ban^A.Scer\UAS

interommatidial bristle | supernumerary, with Scer\GAL4^GMR.PF

ban^EP3622

intersegmental nerve, with Scer\GAL4^elav-C155

ban^EP3208

larval segment, with Scer\GAL4^en-e16E

mesothoracic tergum, with Scer\GAL4^ap-md544

ban^EP3622

microchaeta, with Scer\GAL4^ap-md544

ban^EP3622

ommatidium, with Scer\GAL4^GMR.PF

ban^UY3207

pigment cell | supernumerary, with Scer\GAL4^GMR.PF

ban^EP3622

scutellar bristle | ectopic, with Scer\GAL4^sca-537.4

ban^EP3208

tormogen cell, with Scer\GAL4^sca-537.4

ban^EP3208

wing, with Scer\GAL4[-]:

wing, with Scer\GAL4^Bx-MS1096

wing, with Scer\GAL4^en-e16E

wing (with ban^EP3622), with Scer\GAL4[-]:

ban^UY3207

wing (with ban^UY3207), with Scer\GAL4[-]:

ban^EP3622

wing (with Df(3L)ban^Δ1), with Scer\GAL4[-]:

wing (with Df(3L)emc), with Scer\GAL4[-]:

wing | posterior, with Scer\GAL4^en-e16E

wing | posterior compartment, with Scer\GAL4^en-e16E

ban^EP3622

Classical Alleles ( 20 )

For All Classical Alleles Show

Allele of ban	Class	Mutagen	Stocks	Known lesion
ban^L1170a		P-element activity	2	--
ban^EY06207			1	--
ban¹²	amorphic allele - genetic evidence	Delta2-3	0	Yes
ban²⁰	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
ban^B375.1		Delta2-3	0	Yes
ban^B90.1		Delta2-3	0	Yes
ban^E515.1		Delta2-3	0	Yes
ban^E515.1ex		Delta2-3	0	--
ban^EB5-1		Delta2-3	0	Yes
ban^EP3208		P-element activity	0	--
ban^EP3622	hypomorphic allele - genetic evidence	P-element activity	0	Yes
ban^EP3622ex		Delta2-3	0	--
ban^EPg30491	hypomorphic allele - genetic evidence	P-element activity	0	Yes
ban^LA2.5		P-element activity	0	--
ban^LAS55		P-element activity	0	--
ban^unspecified			0	--
ban^UY1079		P-element activity	0	--
ban^UY1678		P-element activity	0	--
ban^UY3207		P-element activity	0	--
ban^UY789		P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of ban	Mutagen	Known lesion
ban^+t6.7	in vitro construct - genomic fragment	Yes
ban^+t7.2	in vitro construct - genomic fragment	Yes
ban^+t9.6	in vitro construct - genomic fragment	Yes
ban^A.Scer\UAS	in vitro construct - regulatory fusion	Yes
ban^{B.Scer\UAS.T:Avic\GFP-EGFP}		Yes
ban^{C.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct	Yes
ban^{D.Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct	Yes
ban^Scer\UAS.cPa	in vitro construct - regulatory fusion	Yes

Aneuploid Aberrations

Disrupted in

Df(3L)ban^Δ1

Df(3L)emc

P{Mae-UAS.6.11}ban^UY1079

Df(3L)emc-E12

(Yang et al., 2009)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

reporter construct

characterization construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{Mae-UAS.6.11}ban^LA2.5

P{Mae-UAS.6.11}ban^LAS55

P{Mae-UAS.6.11}ban^UY789

P{Mae-UAS.6.11}ban^UY1678

P{Mae-UAS.6.11}ban^UY3207

insertion of enhancer trap

P{lacW}ban^L1170a

P{lArB}L61C

Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )

Terms Based on Experimental Evidence ( 12 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

anti-apoptosis

inferred from genetic interaction with W AND inferred from mutant phenotype

(Brennecke et al., 2003, Thompson and Cohen, 2006)

cell proliferation

inferred from mutant phenotype

eye development

inferred from physical interaction with hpo

germ-line stem cell maintenance

gene silencing by miRNA

inferred from direct assay

(Orom et al., 2006)

inferred from mutant phenotype

(Orom et al., 2006)

inferred from mutant phenotype

(Yang et al., 2009)

positive regulation of cell proliferation

inferred from mutant phenotype

positive regulation of multicellular organism growth

inferred from mutant phenotype

regulation of apoptosis

inferred from direct assay

(Jaklevic et al., 2008)

inferred from physical interaction with hpo

regulation of cell proliferation

inferred from physical interaction with hpo

regulation of dendrite development

inferred from mutant phenotype

(Parrish et al., 2009)

regulation of gene silencing

inferred from mutant phenotype

(Schneiderman et al., 2010)

regulation of multicellular organism growth

inferred from mutant phenotype

(He and Hannon, 2004, Bergmann and Lane, 2003)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 7 terms )

Molecular Function

CV term

Evidence

References

mRNA 3'-UTR binding

traceable author statement

Biological Process

CV term

Evidence

References

anti-apoptosis

non-traceable author statement

(Ambros, 2003)

traceable author statement

(Bergmann and Lane, 2003, Xu et al., 2004)

cell growth

traceable author statement

(Xu et al., 2004)

negative regulation of translation

traceable author statement

(He and Hannon, 2004)

negative regulation of translation involved in gene silencing by miRNA

traceable author statement

(Gesellchen and Boutros, 2004, Nakahara and Carthew, 2004)

positive regulation of cell proliferation

traceable author statement

(Ambros, 2003, Bergmann and Lane, 2003)

positive regulation of growth

traceable author statement

(Bergmann and Lane, 2003)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

miRNA_gene

(Brennecke et al., 2003, FlyBase Genome Annotators, 2010)

nuclear_gene

(Brennecke et al., 2003, FlyBase Genome Annotators, 2010)

Interactions & Pathways

Summary of Physical Interactions

Protein-protein

Interacting group

Assay

References

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

ban allele

Gene

References

ban^EP3622

rpr

ban^UY789

ban^UY1079

ban^UY1678

ban^UY3207

unspecified

CycE

E2f

(Mueller et al., 2005)

hpo

p53

(Mueller et al., 2005)

External Data

DroID - A comprehensive database of gene and protein interactions.

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FBgn0262451

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dpse\ban

y¹ w^67c23; P{EPgy2}ban^EY06207

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Bloomington

16391

10154

y¹ w¹¹¹⁸; P{lacW}ban^L1170a, l(3)L1170b^L1170b/TM3, Ser¹

Kyoto

111004

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}ban^L1170a P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 0 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

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3346233

Other Information

Discoverer

Etymology

The gene is named "bantam" after the mutant phenotype; mutant flies are smaller than normal.

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: ban EP3208

(FlyBase Genome Annotators, 2010)

Source for database merge of

Additional comments

The annotation for ban has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. The symbol of the annotation has been changed from CR33559 to CR43018 to reflect this change.

"BEST:RE64518" does not encode "ban" function.

Evidence suggests that the P{EP}EP3219[EP3219] insertion is probably not an allele of "ban" but instead affects a genetically separable locus; the decrease in wing size seen in EP3219[EP3219]/Df(3L)ban[Δ1] flies is due primarily to a reduction in cell size rather than cell number, in contrast to ban[L1170a]/Df(3L)ban[Δ1], ban[EP3622]/Df(3L)ban[Δ1] or ban[EPg30491]/Df(3L)ban[Δ1] flies, where the decrease in wing size is due to a reduction in the number of cells. In addition, EP3219[EP3219] combined with ban[L1170a], ban[EPg30491] or ban[EP3622] has little or no effect on growth.

Other Comments

ban limits IR-induced apoptosis in larval imaginal discs.

(Jaklevic et al., 2008)

Upregulation of ban miRNA suppresses neurodegeneration.

(Bilen et al., 2006)

ban activity defines distinct targets that modulate neuro-degeneration.

(Bilen et al., 2006)

ban is a target of the hpo signalling pathway.

ban suppresses W expression.

(Thompson and Cohen, 2006)

ban mutant clones grow poorly due to a reduced rate of cell proliferation.

ban acts downstream of yki in the hpo pathway.

(Thompson and Cohen, 2006)

New annotation (CR33559) in release 4.1 of the genome annotation.

(FlyBase Genome Annotators, 2005)

ban microRNA simultaneously stimulates cell proliferating and prevent apoptosis.

(Büssing et al., 2010, Okamura et al., 2009)

External Crossreferences & Linkouts

Sequence Crossreferences

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

3772549

Other Crossreferences

miRBase - the home of microRNA data

•

MI0000387

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262451

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0262451

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

3346233

modMine - Data generated by the modENCODE project.

•

FBgn0262451

Synonyms & Secondary IDs ( 12 )

Reported As

Symbol Synonym

bantam

cot

(FlyBase Genome Annotators, 2005, Schneiderman et al., 2010)

CR33559

CR43018

(FlyBase Genome Annotators, 2010, )

EP3208

(Abdelilah-Seyfried et al., 2000, )

unnamed

(Kraut et al., 2001)

Name Synonym

bantam

(Sanders and Smith, 2011, Qian et al., 2011, Ameres et al., 2010, Czech et al., 2008, Hipfner et al., 2002, Reynolds, 2007, Thompson and Cohen, 2006, Vagin et al., 2006, Nolo et al., 2006, Orom et al., 2006, Stark et al., 2005, Morrison and Halder, 2010, Shcherbata et al., 2007, Neumueller et al., 2007, Garcia et al., 2007, Nurminsky, 2007, Förstemann et al., 2007, Shcherbata et al., 2008, Reynolds et al., 2008, Horwich et al., 2007, Bilen et al., 2006, Rehwinkel et al., 2006, Neumuller et al., 2008, Herranz et al., 2008, Zhang et al., 2008, Okamura et al., 2008, Kawamura et al., 2008, Barbee et al., 2006, Berdnik et al., 2008, Stofanko et al., 2008, Dutta and Baehrecke, 2008, Jaklevic et al., 2008, Tanaka-Matakatsu et al., 2009, Kadener et al., 2009, Yang et al., 2009, Flynt et al., 2009, Nahvi et al., 2009, Eulalio et al., 2009, Semeshin et al., 2008, Yu et al., 2009, Hamaratoglu et al., 2009, Sabin et al., 2009, Pek et al., 2009, Peng et al., 2009, Kadener et al., 2009, Cziko et al., 2009, Rehmsmeier et al., 2004, Easow et al., 2007, Liu et al., 2007, Park et al., 2007, Martin et al., 2009, Parrish et al., 2009, Lee et al., 2009, Berdnik et al., 2008, Robinson et al., 2010, Genevet et al., 2009, Herranz et al., 2010, Flynt et al., 2010, Ruby et al., 2007, Milton et al., 2010, Ghildiyal et al., 2010, Schneiderman et al., 2010, Shen et al., 2009, Gehrke et al., 2010, Shah and Förstemann, 2008, Enright et al., 2003)

Bantam

(He and Hannon, 2004, Zhou et al., 2008)

clochette