Gene Dmel\mir-100

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General Information

Symbol

Dmel\mir-100

Species

D. melanogaster

Name

mir-100 stem loop

Annotation symbol

CR43044

Feature type

miRNA_gene

FlyBase ID

FBgn0262463

Gene Model Status

Current

Stock availability

1 publicly available

Also Known As

Dme-miR-100

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

36F5-36F5

Sequence location

2L:18,471,434..18,471,533 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene mir-100 stem loop is referred to in FlyBase by the symbol Dmel\mir-100 (CR43044, FBgn0262463). It is a miRNA_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: cellular response to ecdysone. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript. Gene sequence location is 2L:18471434..18471533.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36F5-36F5

Limits computationally determined from genome sequence between P{lacW}Aac11^k06710 and P{EP}CG10413^EP2164 36E3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

36E5-36F2

(Sempere et al., 2003)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\mir-100 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Locations and structures of miRNA models as mapped by miRBase (FBrf0220601).

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

FBtr0304523

100

FBtr0304524

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

miRBase - the home of microRNA data

•

MI0000378

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage -- adult stage

(Sempere et al., 2003)

pupal stage -- adult stage

organism

(Aravin et al., 2003)

Additional Descriptive Data

Expression is stronger in adults than in pupae.

(Aravin et al., 2003)

Northern analysis of 24 microRNAs showed that mir-100, mir-125 and let-7 transcripts display the same pattern of expression and can be detected from pre-pupariation through adulthood. No transcript is detected in earlier stage.

(Sempere et al., 2003)

Marker for

Subcellular Localization

CV Term

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Treatments RNA-Seq

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(Graveley et al., 2011)

mE1_20_mRNA_expression_cluster_08

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^Act5C.PU

mir-100^Scer\UAS.cSa

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of mir-100	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Allele of mir-100	Mutagen	Stocks	Known lesion
mir-100^{Scer\UAS.T:Ppyr\LUC}	in vitro construct	1	Yes
mir-100^+tSa	in vitro construct - genomic fragment	0	Yes
mir-100^{let-7-C.Scer\UAS.cSa}	in vitro construct - regulatory fusion	0	Yes
mir-100^{Scer\UAS.cluster.cLa}	in vitro construct - regulatory fusion	0	Yes
mir-100^Scer\UAS.cSa	in vitro construct - regulatory fusion	0	Yes
mir-100^{Scer\UAS.T:Disc\RFP}	in vitro construct	0	Yes
mir-100^Δ.cSa	in vitro construct - deletion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)let-7-C^KO1

(Sokol et al., 2008)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

M{UAS-mir-100.S}

P{UAS-DsRed-mir-100.L}

P{UAS-let-7-C}

P{UAS-LUC-mir-100.T}

P{UAS-mir-100+mir-125+let-7.L}

characterization construct

P{W8,let-7-C}

P{W8,let-7-C^Δlet-7}

P{W8,let-7-C^ΔmiR-100}

P{W8,let-7-C^ΔmiR-125}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 1 unique term )

Terms Based on Experimental Evidence ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

cellular response to ecdysone

inferred from mutant phenotype

(Chawla and Sokol, 2012)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

miRNA_gene

(Sokol and Ambros, 2003, FlyBase Genome Annotators, 2010)

nuclear_gene

(Sokol and Ambros, 2003, FlyBase Genome Annotators, 2010)

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

mir-100 allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262463

Orthologs

OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

No orthologs identified

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (None identified)

No orthologies identified

Orthologs in non-Drosophila Dipterans (None identified)

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (None identified)

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (0) based on analysis using Dmel annotation version 4.3

No orthologs identified

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

Bloomington

41166

w¹¹¹⁸; P{UAS-LUC-mir-100.T}attP2

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 0 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

12798011

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

The annotation for mir-100 has been changed in release 5.31 of the genome annotation, so that instead of representing a mature miRNA it now represents the precursor stem loop pre-miRNA. The symbol of the annotation has been changed from CR33313 to CR43044 to reflect this change.

(FlyBase Genome Annotators, 2010)

The three microRNAs let-7, mir-125 and mir-100 are co-transcribed as a single polycistronic transcription unit (the "let-7-Complex, let-7-C").

(Sokol et al., 2008)

Other Comments

New annotation (CR33313) in release 3.2 of the genome annotation.

(FlyBase Genome Annotators, 2004)

A long primary transcript comprising all the hairpin precursor sequences for mir-100, let-7 and mir-125 has been detected by RT-PCR. Overall orientation not stated: mir-100+ let-7+ mir-125+

(Aravin et al., 2003)

mir-100, mir-125 and let-7 sequences are clustered within 800bp in 36E5--36F2.

(Sempere et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

Entrez Gene - A searchable database of RefSeq genes.

•

12798011

Other Crossreferences

miRBase - the home of microRNA data

•

MI0000378

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262463

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0262463

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

12798011

modMine - Data generated by the modENCODE project.

•

FBgn0262463

Synonyms & Secondary IDs ( 7 )

Reported As

Symbol Synonym

CR33313

CR43044

(FlyBase Genome Annotators, 2010, )

Dme-miR-100

(Zhou et al., 2009)

dme-mir-100

(Ryazansky et al., 2011, Huang and Gu, 2007, Reinhardt et al., 2012)

miR-100

(Bejarano et al., 2012, Lawlor et al., 2011, Liu et al., 2011, Leaman, 2005, Sokol and Ambros, 2005, Nakahara and Carthew, 2004, Lai et al., 2003, Sempere et al., 2003, Stark et al., 2003, Stark et al., 2003, Behura, 2007, Tadros et al., 2007, Caygill and Johnston, 2008, Sokol et al., 2008, Griffiths-Jones et al., 2011, Ibáñez-Ventoso et al., 2008, Chawla and Sokol, 2012, Wu et al., 2012)

mir-100

(Schertel et al., 2012, FlyBase Genome Annotators, 2010, Neumuller et al., 2008, Sokol et al., 2008, Ruby et al., 2007, Stark et al., 2007, Sokol et al., 2008, Bejarano et al., 2012)

Name Synonym

mir-100

mir-100 stem loop

Secondary FlyBase IDs

FBgn0053313
FBgn0064160
FBgn0262337

References ( 35 )

Generate a list of

All references relating to this gene ( 35 )

List References by type

Recent research papers ( 9 )

Chawla and Sokol, 2012, Development 139(10): 1788--1797: Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function. [FBrf0218077]
Marrone et al., 2012, BMC Cell Biol. 13: 26: Dg-Dys-Syn1 signaling in Drosophila regulates the microRNA profile. [FBrf0220581]
Reinhardt et al., 2012, Front. Genet. 3: 226: Lack of miRNA Misregulation at Early Pathological Stages in Drosophila Neurodegenerative Disease Models. [FBrf0219876]
Schertel et al., 2012, Genetics 192(4): 1543--1552: Functional Characterization of Drosophila microRNAs by a Novel in Vivo Library. [FBrf0220194]
Wu et al., 2012, Dev. Cell 23(1): 202--209: let-7-Complex MicroRNAs Regulate the Temporal Identity of Drosophila Mushroom Body Neurons via chinmo. [FBrf0218990]
Griffiths-Jones et al., 2011, EMBO Rep. 12(2): 172--177: MicroRNA evolution by arm switching. [FBrf0212915]
Lawlor et al., 2011, Hum. Mol. Genet. 20(19): 3757--3768: Double-stranded RNA is pathogenic in Drosophila models of expanded repeat neurodegenerative diseases. [FBrf0215240]
Liu et al., 2011, Curr. Biol. 21(22): 1888--1893: The Exoribonuclease Nibbler Controls 3' End Processing of MicroRNAs in Drosophila. [FBrf0216782]
Ryazansky et al., 2011, BMC Genomics 12: 371: Evidence for post-transcriptional regulation of clustered microRNAs in Drosophila. [FBrf0214628]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2011

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