FB2025_01 , released February 20, 2025
Gene: Dmel\VhaAC45
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General Information
Symbol
Dmel\VhaAC45
Species
D. melanogaster
Name
Vacuolar H+ ATPase AC45 accessory subunit
Annotation Symbol
CG8029
Feature Type
protein_coding_gene
FlyBase ID
FBgn0262515
Gene Model Status
Current
Stock Availability
11 publicly available
Gene Summary
Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. (UniProt, Q7JR49)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

ATP6AP1, Vacuolar H+ ATPase accessory protein AC45

Key Links
Genomic Location
Cytogenetic map
45B4-45B4
Sequence location
Recombination map
2-60
RefSeq locus
NT_033778 REGION:9207561..9209774
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables ATPase activator activity
inferred from biological aspect of ancestor with PANTHER:PTN007576952
(GO Reference Genome Project, 2011-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in vacuolar proton-transporting V-type ATPase complex assembly
inferred from genetic interaction with UniProtKB:Q9VHG4
(Guida et al., 2018)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in regulation of cellular pH
inferred from biological aspect of ancestor with PANTHER:PTN007576952
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
part_of vacuolar proton-transporting V-type ATPase complex
inferred from mutant phenotype
(Guida et al., 2018)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of proton-transporting V-type ATPase complex
inferred from biological aspect of ancestor with PANTHER:PTN007576952
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the vacuolar ATPase subunit S1 family. (Q7JR49)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
VACUOLAR ATPASE ACCESSORY SUBUNITS -
Accessory subunits of the V-ATPases are believed to be non-essential for the function of the proton pump, but act to modify activity. They are not found in unicellular organisms. (Adapted from FBrf0220613).
Protein Function (UniProtKB)
Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles.
(UniProt, Q7JR49)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\VhaAC45 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7JR49)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
VhaAC45-RA
FBtr0088588
NM_165665
1894
379
VhaAC45-RB
FBtr0088587
NM_136626
1925
379
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
VhaAC45-PA
FBpp0087669
41.1
379
4.70
Q7JR49
NP_724770
AAF58965
VhaAC45-PB
FBpp0087668
41.1
379
4.70
Q7JR49
NP_610470
AAF58966
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

379 aa isoforms: VhaAC45-PA, VhaAC45-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Accessory component of the multisubunit proton-transporting vacuolar (V)-ATPase protein pump (By similarity). May interact with ATP6AP2 (PubMed:29995586).

(UniProt, Q7JR49)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\VhaAC45 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.77

Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of vacuolar proton-transporting V-type ATPase complex
inferred from mutant phenotype
(Guida et al., 2018)
Expression Deduced from Reporters
Reporter:
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis & adult stage | female
nurse cell
• nucleus
(Cooley et al., 2003.5)
follicle cell
• nucleus
(Cooley et al., 2003.5)
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\VhaAC45 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of VhaAC45
Transgenic constructs containing regulatory region of VhaAC45
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\ATP6AP1
9 of 14
Yes
Yes
1  
Hsap\ATP6AP1L
4 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Atp6ap1
7 of 14
Yes
Yes
Rnor\Atp6ap1l
6 of 14
No
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Atp6ap1
8 of 14
Yes
Yes
Mmus\Atp6ap1l
5 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\atp6ap1.2
8 of 13
Yes
Yes
Xtro\LOC100487454
6 of 13
No
Yes
Xtro\atp6ap1l
3 of 13
No
Yes
Xtro\amer2
1 of 13
No
Yes
Danio rerio (Zebrafish) (4)
Drer\atp6ap1a
8 of 14
Yes
Yes
Drer\atp6ap1b
8 of 14
Yes
Yes
Drer\atp6ap1la
7 of 14
No
Yes
Drer\atp6ap1lb
6 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\vha-19
5 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP003879
12 of 12
Yes
Yes
Agam\AgaP_AGAP001624
2 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:VhaAC45. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (2)
CG5421
4 of 13
VhaAC45RP
4 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    ATP6AP1; ATPase H+ transporting accessory protein 1
    model of  immunodeficiency 47
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ATP6AP1; ATPase H+ transporting accessory protein 1
    9 of 14
    300197
     
    ATP6AP1L; ATPase H+ transporting accessory protein 1 like (pseudogene)
    4 of 14
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        Other Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Accessory component of the multisubunit proton-transporting vacuolar (V)-ATPase protein pump (By similarity). May interact with ATP6AP2 (PubMed:29995586).
        (UniProt, Q7JR49 )
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map
        2-60
        Cytogenetic map
        45B4-45B4
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        45B4-45B4
        Left limit from in situ hybridisation (FBrf0157097) Right limit from in situ hybridisation (FBrf0157097)
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        45B4-45B4
        (Oh et al., 2003)
        Experimentally Determined Recombination Data
        Location

        2-60

        (FlyBase, 2016)

        2-58.2

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (11)
        Genomic Clones (20)
        cDNA Clones (179)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            6 alleles of l(2)SH1634 recovered in a P-insertion screen.

            (Oh et al., 2003)

            Area matching Drosophila EST AI106957. This EST forms a 1756bp contig with ESTs AA391125, AA567307 and AA735971. Probable intron in gene represented by EST AI106957.

            (Davies, 2001.3.15)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: CG8029 anon-WO0118547.117

            (FlyBase, 1996-)

            Source for merge of: CG8029 l(2)SH1634

            (Allan et al., 2005)
            Additional comments

            Source for merge of CG8029 anon-WO0118547.117 was sequence comparison ( date:051113 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of

            Source for identity of: VhaAC45 CG8029

            (Allan et al., 2005)
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (10)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 46 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (60)