FlyBase Gene Report: Dmel\Rab8

General Information

Symbol

Dmel\Rab8

Species

D. melanogaster

Name

Rab8

Annotation Symbol

CG8287

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262518

Gene Model Status

Current

Stock Availability

18 publicly available

Gene Summary

Rab8 (Rab8) encodes a member of the Rab GTPase family that regulates the function of the recycling endosome, where it localizes. [Date last reviewed: 2019-05-16] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

l(3)76BDu, dm-Rab8, DmRab8

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:19,867,488..19,871,078 [-]

Recombination map

3-46

RefSeq locus

NT_037436 REGION:19867488..19871078

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (24 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTP binding

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR003579, InterPro:IPR005225

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables GTPase activity

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

Biological Process (12 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in polarized secretion of basement membrane proteins in epithelium

inferred from mutant phenotype

(Devergne et al., 2017)

involved_in protein localization to membrane

inferred from mutant phenotype

(Mavor et al., 2016)

involved_in regulation of synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(West et al., 2015)

involved_in viral entry into host cell

inferred from high throughput mutant phenotype

(Hodgson et al., 2019)

Terms Based on Predictions or Assertions (8 terms)

CV Term

Evidence

References

involved_in cellular response to insulin stimulus

inferred from biological aspect of ancestor with PANTHER:PTN000635544

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in Golgi vesicle fusion to target membrane

inferred from biological aspect of ancestor with PANTHER:PTN000635544

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein localization to plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000635134

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein secretion

inferred from biological aspect of ancestor with PANTHER:PTN000635132

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in Rab protein signal transduction

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

involved_in regulation of exocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000635132

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in vesicle docking involved in exocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000635132

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in vesicle-mediated transport

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

Cellular Component (10 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Mavor et al., 2016)

located_in endosome

inferred from direct assay

(Devergne et al., 2017)

located_in Golgi apparatus

inferred from direct assay

(Devergne et al., 2017, Mavor et al., 2016)

located_in neuronal cell body

inferred from direct assay

(Jin et al., 2012)

located_in plasma membrane

inferred from direct assay

(Mavor et al., 2016)

located_in recycling endosome

inferred from direct assay

(Mavor et al., 2016, West et al., 2015)

inferred from high throughput direct assay

(Chan et al., 2011)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

inferred from direct assay

(West et al., 2015, Jin et al., 2012)

located_in synaptic vesicle

inferred from high throughput direct assay

(Chan et al., 2011)

located_in vesicle

inferred from direct assay

(Mavor et al., 2016)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

is_active_in endosome

inferred from biological aspect of ancestor with PANTHER:PTN000635134

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000635134

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in synaptic vesicle

inferred from biological aspect of ancestor with PANTHER:PTN001530779

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in trans-Golgi network transport vesicle

inferred from biological aspect of ancestor with PANTHER:PTN000635544

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in vesicle

inferred from sequence or structural similarity with FLYBASE:Rab11; FB:FBgn0015790

(FlyBase Curators, 2008--)

Gene Group (FlyBase)

RAB GTPASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Rab8 (Rab8) encodes a member of the Rab GTPase family that regulates the function of the recycling endosome, where it localizes. [Date last reviewed: 2019-05-16]

Gene Group (FlyBase)

RAB GTPASES -: The Rab family are members of the Ras superfamily of small GTPases. Rabs regulate vesicle trafficking including cargo selection, vesicle budding, transport, docking and targeting. They localize to different intracellular compartments directed by specific isoprenylation of C-terminal motifs. (Adapted from PMID:15731001).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rab8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.40

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

gene

(Zhu et al., 2005)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rab8-RA

FBtr0074921

NM_079448

2263

207

Rab8-RB

FBtr0308832

NM_001259907

1068

172

Rab8-RC

FBtr0308833

NM_001259908

2043

207

Additional Transcript Data and Comments

Reported size (kB)

2.2 (longest cDNA)

(Satoh et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Rab8-PA

FBpp0074690

23.7

207

8.77

NP_524172

AAF49101

Rab8-PB

FBpp0300989

19.7

172

8.67

NP_001246836

AFH04507

Rab8-PC

FBpp0300990

23.7

207

8.77

NP_001246837

AFH04508

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

207 aa isoforms: Rab8-PA, Rab8-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

207 (aa)

(Satoh et al., 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rab8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

head mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

plasmatocyte

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Mavor et al., 2016)

located_in endosome

inferred from direct assay

(Devergne et al., 2017)

located_in Golgi apparatus

inferred from direct assay

(Devergne et al., 2017, Mavor et al., 2016)

located_in neuronal cell body

inferred from direct assay

(Jin et al., 2012)

located_in plasma membrane

inferred from direct assay

(Mavor et al., 2016)

located_in recycling endosome

inferred from direct assay

(Mavor et al., 2016, West et al., 2015)

inferred from high throughput direct assay

(Chan et al., 2011)

located_in synapse

inferred from high throughput direct assay

(Chan et al., 2011)

inferred from direct assay

(West et al., 2015, Jin et al., 2012)

located_in synaptic vesicle

inferred from high throughput direct assay

(Chan et al., 2011)

located_in vesicle

inferred from direct assay

(Mavor et al., 2016)

Expression Deduced from Reporters

Reporter: PBac{I-SceI(FRT.Rab8-GAL4.ATG(loxP.3xP3-RFP))}

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P6 -- P7

neuron of pupal brain

Comment: 30-40h APF

(Chan et al., 2011)

mushroom body

Comment: 30-40h APF

(Chan et al., 2011)

neuropil glial sheath

Comment: 30-40h APF

(Jin et al., 2012)

glial cell of second optic chiasma | intense

Comment: 30-40h APF

(Jin et al., 2012)

neuron of adult optic lobe | faint

Comment: 30-40h APF

(Jin et al., 2012)

mushroom body | intense

Comment: 30-40h APF

(Jin et al., 2012)

eye photoreceptor cell

Comment: 30-40h APF

(Jin et al., 2012)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rab8 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rab8

View exonic expression by tissue for Dmel\Rab8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262518

DRscDB

40168/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0262518

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8287

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rab8

Transgenic constructs containing regulatory region of Rab8

Aberrations (Deficiencies and Duplications) ( 27 )

Inferred from experimentation ( 27 )

Gene disrupted in

Gene not disrupted in

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

Rab8^GD12520

lethal | recessive

Rab8¹

lethal - all die before end of pupal stage

Rab8¹

lethal - all die during immature adult stage (with Rab8¹)

lethal - all die during immature adult stage (with Rab8²)

Rab8¹

lethal - all die during immature adult stage (with Rab8^B229)

Rab8¹

viable

Rab8^EYFP

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Rab8^GD12520

viable, with Scer\GAL4^Mef2.PR

Rab8^GD12520

viable, with Scer\GAL4^tin.CΔ4

Rab8^GD12520

Other Phenotypes

Allele

abnormal neuroanatomy | third instar larval stage, with Rab8¹/Rab8^B229, Scer\GAL4^Act5C.PI

Rab8^UAS.cWa

abnormal neuroanatomy | third instar larval stage, with Rab8¹/Rab8^B229, Scer\GAL4^Mhc.PW

Rab8^UAS.cWa

abnormal neuroanatomy | third instar larval stage, with Rab8¹/Rab8^B229, Scer\GAL4^nSyb.PS

Rab8^UAS.cWa

abnormal neuroanatomy | third instar larval stage (with Df(3L)ED228)

abnormal neuroanatomy | third instar larval stage (with Rab8¹)

abnormal neuroanatomy | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Act5C.PI

Rab8^B229

abnormal neuroanatomy | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

Rab8^B229

abnormal neuroanatomy | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

Rab8^B229

abnormal neuroanatomy | third instar larval stage (with Rab8²)

abnormal neuroanatomy | third instar larval stage (with Rab8³)

abnormal neuroanatomy | third instar larval stage (with Rab8^B229)

abnormal neuroanatomy | third instar larval stage (with Rab8^B229), with Rab8^UAS.cWa, Scer\GAL4^Act5C.PI

Rab8¹

abnormal neuroanatomy | third instar larval stage (with Rab8^B229), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

Rab8¹

abnormal neuroanatomy | third instar larval stage (with Rab8^B229), with Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

Rab8¹

abnormal neuroanatomy | third instar larval stage (with Rab8^Z3007)

abnormal neurophysiology | third instar larval stage (with Rab8¹)

Rab8^B229

abnormal neurophysiology | third instar larval stage (with Rab8^B229)

Rab8¹

some die during pupal stage

Rab8¹

Phenotype manifest in

Allele

A1-7 lateral longitudinal muscle 1 | third instar larval stage, with Rab8¹/Rab8^B229, Scer\GAL4^Act5C.PI

Rab8^UAS.cWa

A1-7 lateral longitudinal muscle 1 | third instar larval stage, with Rab8¹/Rab8^B229, Scer\GAL4^Mhc.PW

Rab8^UAS.cWa

A1-7 lateral longitudinal muscle 1 | third instar larval stage, with Rab8¹/Rab8^B229, Scer\GAL4^nSyb.PS

Rab8^UAS.cWa

A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8¹)

Rab8^B229

A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Act5C.PI

Rab8^B229

A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

Rab8^B229

A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

Rab8^B229

A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8^B229)

Rab8¹

A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8^B229), with Rab8^UAS.cWa, Scer\GAL4^Act5C.PI

Rab8¹

A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8^B229), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

Rab8¹

A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Rab8^B229), with Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

Rab8¹

A1-7 ventral longitudinal muscle 3 | third instar larval stage, with Rab8¹/Rab8^B229, Scer\GAL4^Mhc.PW

Rab8^UAS.cWa

A1-7 ventral longitudinal muscle 3 | third instar larval stage (with Rab8¹)

Rab8^B229

A1-7 ventral longitudinal muscle 3 | third instar larval stage (with Rab8¹), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

Rab8^B229

A1-7 ventral longitudinal muscle 3 | third instar larval stage (with Rab8^B229)

Rab8¹

A1-7 ventral longitudinal muscle 3 | third instar larval stage (with Rab8^B229), with Rab8^UAS.cWa, Scer\GAL4^Mhc.PW

Rab8¹

embryo, with Avic\GFP^{dsRNA.shRNA.UAS.V20.1}, Scer\GAL4^{VP16.mat.αTub67C}

Rab8^EGFP

embryo, with Scer\GAL4^{VP16.mat.αTub67C}, slmb^{UASp.cHa.vhhGFP4}

Rab8^EGFP

embryonic/larval motor neuron | third instar larval stage (with Rab8¹)

Rab8^B229

embryonic/larval motor neuron | third instar larval stage (with Rab8^B229)

Rab8¹

embryonic/larval neuromuscular junction | third instar larval stage (with Df(3L)ED228)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8¹)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8²)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8³)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8^B229)

embryonic/larval neuromuscular junction | third instar larval stage (with Rab8^Z3007)

follicle cell, with Scer\GAL4^GR1

Rab8^{T22N.UASp.YFP}

follicle cell, with Scer\GAL4^tj.PU

Rab8^GD12520

follicle cell | somatic clone

Rab8¹

larval ventral nerve cord | third instar larval stage (with Rab8¹)

Rab8^B229

larval ventral nerve cord | third instar larval stage (with Rab8^B229)

Rab8¹

NMJ bouton | third instar larval stage (with Df(3L)ED228)

NMJ bouton | third instar larval stage (with Rab8¹)

NMJ bouton | third instar larval stage (with Rab8²)

NMJ bouton | third instar larval stage (with Rab8³)

NMJ bouton | third instar larval stage (with Rab8^B229)

NMJ bouton | third instar larval stage (with Rab8^Z3007)

ovarian basement membrane | oogenesis, with Scer\GAL4^GR1

Rab8^{T22N.UASp.YFP}

ovarian basement membrane | oogenesis, with Scer\GAL4^tj.PU

Rab8^GD12520

ovarian basement membrane | oogenesis | somatic clone

Rab8¹

recycling endosome (with Rab8¹)

Rab8^B229

recycling endosome (with Rab8^B229)

Rab8¹

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (20)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RAB8A

13 of 15

Yes

Hsap\RAB8B

12 of 15

Yes

Hsap\RAB10

5 of 15

Hsap\RAB13

5 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\RASEF

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (18)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rab8a

13 of 15

Yes

Mmus\Rab8b

11 of 15

Yes

Mmus\Rab10

5 of 15

Mmus\Rab13

4 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Rab35

1 of 15

Mmus\Rab40b

1 of 15

Mmus\Rab44

1 of 15

Yes

Mmus\Rasef

1 of 15

Yes

Rattus norvegicus (Norway rat) (20)

Rnor\Rab8b

10 of 13

Yes

Rnor\Rab8a

9 of 13

Yes

Rnor\Rab10

3 of 13

Rnor\Rab13

3 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Rasef

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (20)

Xtro\rab8b

11 of 12

Yes

Xtro\rab8a

10 of 12

Yes

Xtro\rab13

4 of 12

Yes

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\rasef

1 of 12

Yes

Xtro\rasef

1 of 12

Yes

Danio rerio (Zebrafish) (28)

Drer\rab8a

11 of 15

Yes

Drer\rab8b

11 of 15

Yes

Drer\rab10

5 of 15

Drer\rab13

5 of 15

Yes

Drer\LOC100535415

3 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

Yes

Drer\cracr2ab

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\si:dkey-16l2.16

1 of 15

Drer\zgc:162879

1 of 15

Yes

Drer\zgc:171927

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\rab-8

14 of 15

Yes

Cele\rab-10

4 of 15

Cele\K02E10.1

2 of 15

Cele\4R79.2

1 of 15

Yes

Cele\rab-1

1 of 15

Cele\rab-3

1 of 15

Cele\rab-28

1 of 15

Yes

Cele\rab-35

1 of 15

Cele\rsef-1

1 of 15

Yes

Cele\Y71H2AM.12

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (6)

Atha\AT5G59840

9 of 9

Yes

Atha\RAB8

8 of 9

Yes

Atha\RABE1c

8 of 9

Yes

Atha\RAB8C

7 of 9

Atha\RABE1e

7 of 9

Atha\RABG1

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\SEC4

12 of 15

Yes

Scer\YPT1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\ypt2

11 of 12

Yes

Spom\ypt1

1 of 12

Spom\ypt4

1 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190FFX )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA23499

Drosophila persimilis

Dper\GL15675

Drosophila willistoni

Dwil\GK17214

Drosophila virilis

Dvir\GJ13999

Drosophila mojavensis

Dmoj\GI13664

Drosophila grimshawi

Dgri\GH16908

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150AK5 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018030

Glossina morsitans

Tsetse fly

Gmor\GMOY011233

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09312

Mayetiola destructor

Hessian fly

Mdes\Mdes010352

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010170

Anopheles darlingi

American malaria mosquito

Adar\ADAC009251

Anopheles gambiae

Malaria mosquito

Agam\AGAP001211

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ006714

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0C4Y )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA004846

Bombyx mori

Silkmoth

Bmor\BGIBMGA004666

Danaus plexippus

Monarch butterfly

Dple\DPOGS212348-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS214949-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015585

Apis florea

Little honeybee

Aflo\2611050260

Apis florea

Little honeybee

Aflo\3052449492

Apis mellifera

Western honey bee

Amel\Rab10

Apis mellifera

Western honey bee

Amel\GB55960

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10072

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP16574

Bombus terrestris

Buff-tailed bumblebee

Bter\15208171

Bombus terrestris

Buff-tailed bumblebee

Bter\15213936

Linepithema humile

Argentine ant

Lhum\LH19264

Linepithema humile

Argentine ant

Lhum\LH20433

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002776

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0002c2

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011245

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001698

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12623

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07788

Tribolium castaneum

Red flour beetle

Tcas\TC014518

Tribolium castaneum

Red flour beetle

Tcas\TC002919

Pediculus humanus

Human body louse

Phum\PHUM450640

Pediculus humanus

Human body louse

Phum\PHUM042170

Rhodnius prolixus

Kissing bug

Rpro\RPRC006938

Rhodnius prolixus

Kissing bug

Rpro\RPRC013915

Cimex lectularius

Bed bug

Clec\CLEC002544

Cimex lectularius

Bed bug

Clec\CLEC007559

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001895

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001670

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11867

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01565

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0DM6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008252

Strigamia maritima

European centipede

Smar\SMAR010035

Ixodes scapularis

Black-legged tick

Isca\ISCW024874

Ixodes scapularis

Black-legged tick

Isca\ISCW012955

Stegodyphus mimosarum

African social velvet spider

Smim\KFM75047.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM61149.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur27g01440

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g03710

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_106641

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_301441

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0LA6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rab1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rab8

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rab35

Ciona intestinalis

Vase tunicate

Cint\LOC100185758

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000016136

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000022390

Ciona intestinalis

Vase tunicate

Cint\LOC100185850

Gallus gallus

Domestic chicken

Ggal\RAB8B

Gallus gallus

Domestic chicken

Ggal\RAB10

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028851

Gallus gallus

Domestic chicken

Ggal\RAB35

Gallus gallus

Domestic chicken

Ggal\RAB1A

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (34)

Gene Symbol

Score

Source

Alignment

8 of 10

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 6 )

Allele

Disease

Interaction

References

Rab8^HMS01363

ameliorates lung adenocarcinoma

modeled by Hsap\KIF5B::Hsap\RET^UAS.cDa

(Das and Cagan, 2017)

Rab8^{T22N.UASp.YFP}

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

Rab8^UASp.YFP

ameliorates Parkinson's disease

modeled by Hsap\SNCA^UAS.cKa

(Yin et al., 2014)

modeled by Hsap\SNCA^UAS.cUa

(Yin et al., 2014)

modeled by Hsap\SNCA^TP.UAS.cKa

(Yin et al., 2014)

Rab8¹

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2015)

Rab8^UAS.cWa

ameliorates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2015)

Rab8^dsRNA.UAS

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAB8A; RAB8A, member RAS oncogene family

13 of 15

165040

RAB8B; RAB8B, member RAS oncogene family

12 of 15

613532

RAB10; RAB10, member RAS oncogene family

5 of 15

612672

RAB13; RAB13, member RAS oncogene family

5 of 15

602672

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Rab8 - CG16863

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Rab8 - DZIP1

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Wu et al., 2020)

Rab8 - Tspo

experimental knowledge based

(Guruharsha et al., 2011)

Rab8 - dome

anti tag coimmunoprecipitation, western blot

(Chen et al., 2018)

Rab8 - pdm3

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Rab8 - strat

anti tag coimmunoprecipitation, anti tag western blot

(Devergne et al., 2017)

Rab8 - svp

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Rab8

suppressible

Hrs

(West et al., 2015)

Rab8

suppressible

kay

(West et al., 2015)

Rab8

suppressible

POSH

(West et al., 2015)

Rab8

suppressible

Tak1

(West et al., 2015)

Rab8

suppressible

tkv

(West et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

para

suppressible

Rab8

(Kroll et al., 2015)

strat

suppressible

Rab8

(Bellec et al., 2018, Devergne et al., 2017)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262518

MIST (protein-protein) - An integrated Molecular Interaction Database

Rab8

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

RAB geranylgeranylation

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-46

Cytogenetic map

76D2-76D3

Sequence location

3L:19,867,488..19,871,078 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

76D2-76D3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-46

(FlyBase, 2016)

3-43.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (18)

Bloomington

62546

w¹¹¹⁸; TI{TI}Rab8^EYFP

9782

y¹ w^*; P{UASp-YFP.Rab8}45

27519

y¹ v¹; P{TRiP.JF02669}attP2

9780

y¹ w^*; P{UASp-YFP.Rab8.T22N}12

9781

y¹ w^*; P{UASp-YFP.Rab8.Q67L}05

81899

w^*; PBac{U6:3-Rab8.gRNA}VK00027/TM6B, Tb¹

23271

y¹ w^*; P{UASp-YFP.Rab8.T22N}vimar⁰⁹/SM6a

91625

Rab8² red¹ e⁴/TM6B, Sb¹ Tb¹

23272

y¹ w^*; P{UASp-YFP.Rab8}Dyro⁰⁹/TM3, Sb¹

FlyORF

F003549

M{UAS-Rab8.ORF.3xHA.GW}ZH-86Fb

VDRC

v330424

P{VSH330424}attP40

v28092

w¹¹¹⁸; P{GD12520}v28092/CyO

More stocks available...

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (84)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD44762

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262518

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0262518

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40168

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Rab8 l(3)76BDu

(Cooper et al., 2010)

Additional comments

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Kouranti et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Isolated from a Drosophila head cDNA library.

(Satoh et al., 1997)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 49 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40168

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262518

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262518

DRscDB

40168/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0262518

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8287

FlyMine - An integrated database for Drosophila genomics

FBgn0262518

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40168

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002919

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8287

MARRVEL_MODEL

40168

modMine - A data warehouse for the modENCODE project

FBgn0262518

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262518

DRSC - Results frm RNAi screens

FBgn0262518

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

MIST (protein-protein) - An integrated Molecular Interaction Database

Rab8

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

RAB geranylgeranylation

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

AAF49101

(Armstrong, 2000)

CG8287

(Meadows, 2017.9.14, Dunst et al., 2015, Krahmer et al., 2013, Christis and Munro, 2012, Li et al., 2010, Ni et al., 2010.12.1, Perkins et al., 2009, Sinka et al., 2008, Zhang et al., 2007, Colicelli, 2004, Verstreken and Bellen, 2000.9.4)

DRAB8

(Kohno et al., 1994)

DRab8

(Satoh et al., 1997)

Dm Rab8

(Jekely, 2003)

DmRab8

(Pereira-Leal and Seabra, 2001, Pereira-Leal and Seabra, 2001)

O18338

(Rojas et al., 2012)

Rab-r8

Rab8

(Ma and Brill, 2021, Delfino et al., 2020, González-Méndez et al., 2020, Wu et al., 2020, Yu et al., 2020, Zhao et al., 2020, Han, 2019.5.8, Hodgson et al., 2019, Johnson and Andrew, 2019, Krämer et al., 2019, Bellec et al., 2018, Court et al., 2017, Coutinho-Budd et al., 2017, Devergne et al., 2017, Jewett et al., 2017, Liu et al., 2017, Sánchez-Sánchez et al., 2017, Caviglia et al., 2016, Mavor et al., 2016, Meehan et al., 2016, Vieillard et al., 2016, Kroll et al., 2015, West et al., 2015, White et al., 2015, Garg and Wu, 2014, Gillingham et al., 2014, Gillingham et al., 2014, Thomas and Strutt, 2014, Yashiro et al., 2014, Yin et al., 2014, Zhang et al., 2014, Gradilla and Guerrero, 2013, Krahmer et al., 2013, Kwon et al., 2013, Lindsley et al., 2013, O'Sullivan et al., 2013, Puram and Bonni, 2013, Zhang et al., 2013, Giagtzoglou et al., 2012, Hazelett et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Wang et al., 2012, Callejo et al., 2011, Akimana et al., 2010, Cooper et al., 2010, Warner and Longmore, 2009, Cook, 2008.12.1, Liebl and Featherstone, 2008, Sinka et al., 2008, Haghnia et al., 2007, Stuart et al., 2007, Zhang et al., 2007, Zhang et al., 2007, Cermelli et al., 2006, Kouranti et al., 2006, Wu et al., 2005)

dm-Rab8

(Fukuda, 2004, Fukuda et al., 2004)

l(3)76BDu

(Cooper et al., 2010)

rab8

(Wu et al., 2020, Das and Cagan, 2017, Lammers et al., 2017, Kanamori et al., 2015, Jin et al., 2012, Chan et al., 2011, Mudiganti et al., 2010, Fukuda, 2003.6.21, Ozaki, 1996.4.9)

Name Synonyms

Rab-protein 8

Rab8

l(3)76BDu

Secondary FlyBase IDs