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Gene Dmel\Mi-2

General Information
Symbol	Dmel\Mi-2	Species	D. melanogaster
Name		Annotation symbol	CG8103
Feature type	protein_coding_gene	FlyBase ID	FBgn0262519
Gene Model Status	Current	Stock availability	28 publicly available
Also Known As	dMi-2, Mi2, Pha, dMi2
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	76D3-76D4	Sequence location	3L:19,875,151..19,903,315 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\Mi-2 (CG8103, FBgn0262519). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; nucleosome-dependent ATPase activity; nucleosome binding; chromatin binding; ATPase activity; DNA binding. There is experimental evidence that it is involved in the biological process: dendrite morphogenesis; nucleosome mobilization; muscle organ development; negative regulation of transcription from RNA polymerase II promoter. 61 alleles are reported. The phenotype of these alleles is annotated with: dendrite. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: CHD, C-terminal 2; CHD, N-terminal; Chromo domain; Chromo domain-like; Chromo domain/shadow; DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site; Domain of unknown function DUF1086; Domain of unknown function DUF1087; Helicase, C-terminal; Helicase, superfamily 1/2, ATP-binding domain; SNF2-related; Zinc finger, FYVE/PHD-type; Zinc finger, PHD-finger; Zinc finger, PHD-type; Zinc finger, PHD-type, conserved site; Zinc finger, RING-type; Zinc finger, RING/FYVE/PHD-type. Gene sequence location is 3L:19875151..19903315.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Controlled Vocabulary Terms regulation of transcription from RNA polymerase II promoter
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 76D3-76D4   Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 76D-76D     (Mueller, 1999.1.12) 76D3-76D4   (determined by in situ hybridisation)   (Spradling et al., 1999) 76D-76D   (determined by in situ hybridisation)   (Szeged Stock Center, 1998-) 76D-76D   (determined by in situ hybridisation)   (Kehle et al., 1998) 76D-76D   (determined by in situ hybridisation)   (Deak et al., 1997) 76D1-76D2   (determined by in situ hybridisation)   (Kim et al., 1997) 76D3-76D4     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Mi-2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 DGC clone appears problematic (LD32039): incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mi-2-RA FBtr0074919  NM_140897   6416   1982 Mi-2-RB FBtr0100394  NM_001014591   6486   1983 Mi-2-RC FBtr0302046  NM_001170005   6483   1982 Mi-2-RD FBtr0332152  NM_001275149   6424   1973
Additional Transcript Data & Comments
Reported size (kB)	6.475 (compiled cDNA) (Kehle et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Mi-2-PA   FBpp0074688   224.2   1982   5.33     NP_649154   AAF49099 Mi-2-PB   FBpp0099808   224.3   1983   5.33     NP_001014591   AAX52739 Mi-2-PC   FBpp0291256   224.2   1982   5.33     NP_001163476   ACZ94747 Mi-2-PD   FBpp0304462   223.1   1973   5.48     NP_001262078   AGB94771
Additional Polypeptide Data & Comments
Reported size (kDa)	1982 (aa) (Kehle et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • P-loop containing nucleoside triphosphate hydrolase (IPR027417) SNF2-related (IPR000330) Chromo domain/shadow (IPR000953) Helicase, C-terminal (IPR001650) Zinc finger, PHD-type (IPR001965) DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (IPR002464) Domain of unknown function DUF1086 (IPR009462) Domain of unknown function DUF1087 (IPR009463) Zinc finger, FYVE/PHD-type (IPR011011) CHD, C-terminal 2 (IPR012957) CHD, N-terminal (IPR012958) Zinc finger, RING-type (IPR001841) Helicase, superfamily 1/2, ATP-binding domain (IPR014001) Chromo domain-like (IPR016197) Zinc finger, PHD-finger (IPR019787) Zinc finger, PHD-type, conserved site (IPR019786) Zinc finger, RING/FYVE/PHD-type (IPR013083) Chromo domain (IPR023780)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201994   AA202037   AA202797   AA390464   AA391036   AA392512   AA392547   AA439575   AA440856   AA567615   AA695449   AA697140   AA803820   AA816789   AA820741   AA942480   AA950503   AA951132   AA951524   AA978550   AA990752   AE014296 AAF49099   AE014296 AAX52739   AE014296 ACZ94747   AF119716 AAD17276   AE014296 AGB94771   AI107438   AI135148   AI402336   AI512148   AI512741   AI514925   AI516636   AI516734   AI518399   AI531069   AI542026   AI546623   AQ026242   AQ026314   AY071503   AY113368 AAM29373   BF492156   BG638592   BG638627   BI354251   BI354333   BI372187   BI483934   BI566778   BI585185   BP540913   BP551670   BQ103222   BT149879   CL528083   CL528124   CO156031   CO189448   CO190522   CO263600   CO266160   CO271566   CO279984   CO280706   CO283206   CO284688   CO296061   CO296380   CO297553   CO298518   CO304147   CO304353   CO307170   CO309500   CO310994   CO325256   CO326272   CO327882   CO330680   CO332201   CO339368   CO344142   CZ466713   CZ468437   CZ469920   CZ470539   CZ471099   CZ490803   EC052220   EC063276   EC064741   EC066084   EC069469   EC071610   EC073370   EC075917   EC082179   EC194892   EC196870   EC211233   EC213038   EC214013   EC214991   EC216590   EC217568   EC222134   EC222383   EC223863   EC225520   EC244835   EC247613   EC252856   EC253070   EC256266   EC260026   EC264814   EV581949   FF579569
UniProtKB/Swiss-Prot	O97159
UniProtKB/TrEMBL	Q59E34 E1JI46
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Kehle et al., 1998)
Additional Descriptive Data
	Mi-2 transcript is uniform in preblastoderm embryos. (Kehle et al., 1998)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Kunert et al., 2009) polytene chromosome inferred from direct assay (Murawsky et al., 2001, Reddy et al., 2010)
Expression Deduced from Reporters
Reporter: P{lacW}Mi-2j3D4 Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage   embryonic/larval salivary gland   (Meister and Braun, 1995.10) embryonic/larval foregut   (Meister and Braun, 1995.10) embryonic/larval fat body   (Meister and Braun, 1995.10) embryonic/larval midgut   (Meister and Braun, 1995.10) Malpighian tubule   (Meister and Braun, 1995.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    127 central nervous system, pupae P8    61 head, virgin 1-day female    23 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    28 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    11 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    12 fat body, white prepupae    27 fat body, pupae P8    23 carcass, larvae L3 wandering    75 carcass, 1-day adult    15 carcass, 4-day adult    21 carcass, 20-day adult    16 ovary, virgin 4-day female    119 ovary, mated 4-day female    127 testis, mated 4-day male    21 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (61) central nervous system, larvae L3  (127) central nervous system, pupae P8  (61) head, virgin 1-day female    23 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male  (29) head, mated 4-day male  (33) head, mated 20-day male  (28) salivary gland, larvae L3 wandering    2 salivary gland, white prepupae  (31) digestive system, larvae L3 wandering    16 digestive system, 1-day adult    11 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    12 fat body, white prepupae  (27) fat body, pupae P8    23 carcass, larvae L3 wandering  (75) carcass, 1-day adult    15 carcass, 4-day adult    21 carcass, 20-day adult    16 ovary, virgin 4-day female  (119) ovary, mated 4-day female  (127) testis, mated 4-day male    21 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3  (127) central nervous system, pupae P8    61 head, virgin 1-day female    23 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    28 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    11 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    12 fat body, white prepupae    27 fat body, pupae P8    23 carcass, larvae L3 wandering    75 carcass, 1-day adult    15 carcass, 4-day adult    21 carcass, 20-day adult    16 ovary, virgin 4-day female  (119) ovary, mated 4-day female  (127) testis, mated 4-day male    21 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    127 central nervous system, pupae P8    61 head, virgin 1-day female    23 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    28 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    11 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    12 fat body, white prepupae    27 fat body, pupae P8    23 carcass, larvae L3 wandering    75 carcass, 1-day adult    15 carcass, 4-day adult    21 carcass, 20-day adult    16 ovary, virgin 4-day female    119 ovary, mated 4-day female    127 testis, mated 4-day male    21 accessory gland, mated 4-day male    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    127 central nervous system, pupae P8    61 head, virgin 1-day female    23 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    28 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    11 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    12 fat body, white prepupae    27 fat body, pupae P8    23 carcass, larvae L3 wandering    75 carcass, 1-day adult    15 carcass, 4-day adult    21 carcass, 20-day adult    16 ovary, virgin 4-day female    119 ovary, mated 4-day female    127 testis, mated 4-day male    21 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (61) central nervous system, larvae L3  (127) central nervous system, pupae P8  (61) head, virgin 1-day female    23 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male  29 head, mated 4-day male  (33) head, mated 20-day male  28 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae  31 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    11 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    12 fat body, white prepupae  27 fat body, pupae P8    23 carcass, larvae L3 wandering  (75) carcass, 1-day adult    15 carcass, 4-day adult    21 carcass, 20-day adult    16 ovary, virgin 4-day female  (119) ovary, mated 4-day female  (127) testis, mated 4-day male    21 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3  127 central nervous system, pupae P8    61 head, virgin 1-day female    23 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    28 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    11 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    12 fat body, white prepupae    27 fat body, pupae P8    23 carcass, larvae L3 wandering    75 carcass, 1-day adult    15 carcass, 4-day adult    21 carcass, 20-day adult    16 ovary, virgin 4-day female  119 ovary, mated 4-day female  127 testis, mated 4-day male    21 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    127 central nervous system, pupae P8    61 head, virgin 1-day female    23 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    24 head, mated 4-day female    21 head, mated 20-day female    14 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    28 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    16 digestive system, 1-day adult    11 digestive system, 4-day adult    17 digestive system, 20-day adult    16 fat body, larvae L3 wandering    12 fat body, white prepupae    27 fat body, pupae P8    23 carcass, larvae L3 wandering    75 carcass, 1-day adult    15 carcass, 4-day adult    21 carcass, 20-day adult    16 ovary, virgin 4-day female    119 ovary, mated 4-day female    127 testis, mated 4-day male    21 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    135 embryo 02-04hr    234 embryo 04-06hr    259 embryo 06-08hr    199 embryo 08-10hr    154 embryo 10-12hr    102 embryo 12-14hr    86 embryo 14-16hr    101 embryo 16-18hr    71 embryo 18-20hr    37 embryo 20-22hr    63 embryo 22-24hr    56 larva L1    27 larva L2    29 larva L3 12hr old    24 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    44 white prepupae new    48 white prepupae 12hr    50 white prepupae 24hr    68 pupae 2d postWPP    38 pupae 3d postWPP    20 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    23 adult male 30day    23 adult female 01day    68 adult female 05day    95 adult female 30day    97 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (135) embryo 02-04hr  (234) embryo 04-06hr  (259) embryo 06-08hr  (199) embryo 08-10hr  (154) embryo 10-12hr  (102) embryo 12-14hr  (86) embryo 14-16hr  (101) embryo 16-18hr  (71) embryo 18-20hr  (37) embryo 20-22hr  (63) embryo 22-24hr  (56) larva L1  (27) larva L2  (29) larva L3 12hr old    24 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6  (39) larva L3 puffstage 7-9  (44) white prepupae new  (48) white prepupae 12hr  (50) white prepupae 24hr  (68) pupae 2d postWPP  (38) pupae 3d postWPP    20 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    23 adult male 30day    23 adult female 01day  (68) adult female 05day  (95) adult female 30day  (97) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (135) embryo 02-04hr  (234) embryo 04-06hr  (259) embryo 06-08hr  (199) embryo 08-10hr  (154) embryo 10-12hr  102 embryo 12-14hr    86 embryo 14-16hr  101 embryo 16-18hr    71 embryo 18-20hr    37 embryo 20-22hr    63 embryo 22-24hr    56 larva L1    27 larva L2    29 larva L3 12hr old    24 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    44 white prepupae new    48 white prepupae 12hr    50 white prepupae 24hr    68 pupae 2d postWPP    38 pupae 3d postWPP    20 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    23 adult male 30day    23 adult female 01day    68 adult female 05day    95 adult female 30day    97 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    135 embryo 02-04hr    234 embryo 04-06hr    259 embryo 06-08hr    199 embryo 08-10hr    154 embryo 10-12hr    102 embryo 12-14hr    86 embryo 14-16hr    101 embryo 16-18hr    71 embryo 18-20hr    37 embryo 20-22hr    63 embryo 22-24hr    56 larva L1    27 larva L2    29 larva L3 12hr old    24 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    44 white prepupae new    48 white prepupae 12hr    50 white prepupae 24hr    68 pupae 2d postWPP    38 pupae 3d postWPP    20 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    23 adult male 30day    23 adult female 01day    68 adult female 05day    95 adult female 30day    97 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    135 embryo 02-04hr    234 embryo 04-06hr    259 embryo 06-08hr    199 embryo 08-10hr    154 embryo 10-12hr    102 embryo 12-14hr    86 embryo 14-16hr    101 embryo 16-18hr    71 embryo 18-20hr    37 embryo 20-22hr    63 embryo 22-24hr    56 larva L1    27 larva L2    29 larva L3 12hr old    24 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    44 white prepupae new    48 white prepupae 12hr    50 white prepupae 24hr    68 pupae 2d postWPP    38 pupae 3d postWPP    20 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    23 adult male 30day    23 adult female 01day    68 adult female 05day    95 adult female 30day    97 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (135) embryo 02-04hr  (234) embryo 04-06hr  (259) embryo 06-08hr  (199) embryo 08-10hr  (154) embryo 10-12hr  (102) embryo 12-14hr  (86) embryo 14-16hr  (101) embryo 16-18hr  (71) embryo 18-20hr  (37) embryo 20-22hr  (63) embryo 22-24hr  (56) larva L1  27 larva L2  29 larva L3 12hr old    24 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6  (39) larva L3 puffstage 7-9  (44) white prepupae new  (48) white prepupae 12hr  (50) white prepupae 24hr  (68) pupae 2d postWPP  (38) pupae 3d postWPP    20 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    23 adult male 30day    23 adult female 01day  (68) adult female 05day  (95) adult female 30day  (97) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  135 embryo 02-04hr  (234) embryo 04-06hr  (259) embryo 06-08hr  (199) embryo 08-10hr  (154) embryo 10-12hr  102 embryo 12-14hr    86 embryo 14-16hr  101 embryo 16-18hr    71 embryo 18-20hr    37 embryo 20-22hr    63 embryo 22-24hr    56 larva L1    27 larva L2    29 larva L3 12hr old    24 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    44 white prepupae new    48 white prepupae 12hr    50 white prepupae 24hr    68 pupae 2d postWPP    38 pupae 3d postWPP    20 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    23 adult male 30day    23 adult female 01day    68 adult female 05day    95 adult female 30day    97 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    135 embryo 02-04hr    234 embryo 04-06hr    259 embryo 06-08hr    199 embryo 08-10hr    154 embryo 10-12hr    102 embryo 12-14hr    86 embryo 14-16hr    101 embryo 16-18hr    71 embryo 18-20hr    37 embryo 20-22hr    63 embryo 22-24hr    56 larva L1    27 larva L2    29 larva L3 12hr old    24 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    44 white prepupae new    48 white prepupae 12hr    50 white prepupae 24hr    68 pupae 2d postWPP    38 pupae 3d postWPP    20 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    23 adult male 30day    23 adult female 01day    68 adult female 05day    95 adult female 30day    97 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    97 Schneider line 2 Sg4    111 embryonic 1182-4H    87 embryonic GM2    86 embryonic Kc167    36 embryonic S1    85 embryonic S3    76 leg disc CME L1    136 wing disc CME-W2    68 wing disc ML-DmD8    74 wing disc ML-DmD9    103 wing disc ML-DmD16-c3    99 wing disc ML-DmD21    80 wing disc ML-DmD32    50 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    85 antennal disc ML-DmD20-c5    56 mixed discs ML-DmD4-c1    112 CNS ML-DmBG1-c1    66 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    44 ovary fGS/OSS    112 ovary OSC    116 ovary OSS    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (97) Schneider line 2 Sg4  (111) embryonic 1182-4H  (87) embryonic GM2  (86) embryonic Kc167  (36) embryonic S1  (85) embryonic S3  (76) leg disc CME L1  (136) wing disc CME-W2  (68) wing disc ML-DmD8  (74) wing disc ML-DmD9  (103) wing disc ML-DmD16-c3  (99) wing disc ML-DmD21  (80) wing disc ML-DmD32  (50) haltere disc ML-DmD17-c3  (91) eye-antennal disc ML-DmD11  (85) antennal disc ML-DmD20-c5  (56) mixed discs ML-DmD4-c1  (112) CNS ML-DmBG1-c1  (66) CNS ML-DmBG2-c2  (62) tumorous blood cells mbn2  (44) ovary fGS/OSS  (112) ovary OSC  (116) ovary OSS  (82) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    97 Schneider line 2 Sg4  (111) embryonic 1182-4H    87 embryonic GM2    86 embryonic Kc167    36 embryonic S1    85 embryonic S3    76 leg disc CME L1  (136) wing disc CME-W2    68 wing disc ML-DmD8    74 wing disc ML-DmD9  103 wing disc ML-DmD16-c3    99 wing disc ML-DmD21    80 wing disc ML-DmD32    50 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    85 antennal disc ML-DmD20-c5    56 mixed discs ML-DmD4-c1  (112) CNS ML-DmBG1-c1    66 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    44 ovary fGS/OSS  (112) ovary OSC  (116) ovary OSS    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    97 Schneider line 2 Sg4    111 embryonic 1182-4H    87 embryonic GM2    86 embryonic Kc167    36 embryonic S1    85 embryonic S3    76 leg disc CME L1    136 wing disc CME-W2    68 wing disc ML-DmD8    74 wing disc ML-DmD9    103 wing disc ML-DmD16-c3    99 wing disc ML-DmD21    80 wing disc ML-DmD32    50 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    85 antennal disc ML-DmD20-c5    56 mixed discs ML-DmD4-c1    112 CNS ML-DmBG1-c1    66 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    44 ovary fGS/OSS    112 ovary OSC    116 ovary OSS    82 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    97 Schneider line 2 Sg4    111 embryonic 1182-4H    87 embryonic GM2    86 embryonic Kc167    36 embryonic S1    85 embryonic S3    76 leg disc CME L1    136 wing disc CME-W2    68 wing disc ML-DmD8    74 wing disc ML-DmD9    103 wing disc ML-DmD16-c3    99 wing disc ML-DmD21    80 wing disc ML-DmD32    50 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    85 antennal disc ML-DmD20-c5    56 mixed discs ML-DmD4-c1    112 CNS ML-DmBG1-c1    66 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    44 ovary fGS/OSS    112 ovary OSC    116 ovary OSS    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (97) Schneider line 2 Sg4  (111) embryonic 1182-4H  (87) embryonic GM2  (86) embryonic Kc167  (36) embryonic S1  (85) embryonic S3  (76) leg disc CME L1  (136) wing disc CME-W2  (68) wing disc ML-DmD8  (74) wing disc ML-DmD9  (103) wing disc ML-DmD16-c3  (99) wing disc ML-DmD21  (80) wing disc ML-DmD32  (50) haltere disc ML-DmD17-c3  (91) eye-antennal disc ML-DmD11  (85) antennal disc ML-DmD20-c5  (56) mixed discs ML-DmD4-c1  (112) CNS ML-DmBG1-c1  (66) CNS ML-DmBG2-c2  (62) tumorous blood cells mbn2  (44) ovary fGS/OSS  (112) ovary OSC  (116) ovary OSS  (82) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    97 Schneider line 2 Sg4  111 embryonic 1182-4H    87 embryonic GM2    86 embryonic Kc167    36 embryonic S1    85 embryonic S3    76 leg disc CME L1  136 wing disc CME-W2    68 wing disc ML-DmD8    74 wing disc ML-DmD9  103 wing disc ML-DmD16-c3    99 wing disc ML-DmD21    80 wing disc ML-DmD32    50 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    85 antennal disc ML-DmD20-c5    56 mixed discs ML-DmD4-c1  112 CNS ML-DmBG1-c1    66 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    44 ovary fGS/OSS  112 ovary OSC  116 ovary OSS    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    97 Schneider line 2 Sg4    111 embryonic 1182-4H    87 embryonic GM2    86 embryonic Kc167    36 embryonic S1    85 embryonic S3    76 leg disc CME L1    136 wing disc CME-W2    68 wing disc ML-DmD8    74 wing disc ML-DmD9    103 wing disc ML-DmD16-c3    99 wing disc ML-DmD21    80 wing disc ML-DmD32    50 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    85 antennal disc ML-DmD20-c5    56 mixed discs ML-DmD4-c1    112 CNS ML-DmBG1-c1    66 CNS ML-DmBG2-c2    62 tumorous blood cells mbn2    44 ovary fGS/OSS    112 ovary OSC    116 ovary OSS    82 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    37 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    64 Zinc 5 mM 12 hrs, larvae L3    20 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    84 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    52 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (48) cold shock, 4-day adult  (37) heat shock, 4-day adult  (36) Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult  (39) Cadmium 100 mM 48 hrs, 4-day adult  (87) Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult  (64) Zinc 5 mM 12 hrs, larvae L3    20 Zinc 4.5 mM 48 hrs, 4-day adult  (36) Ethanol 2.5% 3 hrs, larvae L3  (84) Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (73) Caffeine 25 mg/ml 48 hrs, 4-day adult  (44) Paraquat 5 mM 48 hrs, 4-day adult  (52) Paraquat 10 mM 48 hrs, 4-day adult  (59) Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    37 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    64 Zinc 5 mM 12 hrs, larvae L3    20 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    84 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    52 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    37 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    64 Zinc 5 mM 12 hrs, larvae L3    20 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    84 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    52 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    37 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    64 Zinc 5 mM 12 hrs, larvae L3    20 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    84 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    52 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (48) cold shock, 4-day adult  (37) heat shock, 4-day adult  (36) Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult  (39) Cadmium 100 mM 48 hrs, 4-day adult  (87) Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult  (64) Zinc 5 mM 12 hrs, larvae L3    20 Zinc 4.5 mM 48 hrs, 4-day adult  (36) Ethanol 2.5% 3 hrs, larvae L3  (84) Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (73) Caffeine 25 mg/ml 48 hrs, 4-day adult  (44) Paraquat 5 mM 48 hrs, 4-day adult  (52) Paraquat 10 mM 48 hrs, 4-day adult  (59) Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    37 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    64 Zinc 5 mM 12 hrs, larvae L3    20 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    84 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    52 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    48 cold shock, 4-day adult    37 heat shock, 4-day adult    36 Cadmium 50 mM 6 hrs, larvae L3    14 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    39 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    22 Copper 15 mM 48 hrs, 4-day adult    64 Zinc 5 mM 12 hrs, larvae L3    20 Zinc 4.5 mM 48 hrs, 4-day adult    36 Ethanol 2.5% 3 hrs, larvae L3    84 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    44 Paraquat 5 mM 48 hrs, 4-day adult    52 Paraquat 10 mM 48 hrs, 4-day adult    59 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Mi-2CA06598 lethal (with Df(3L)Δ40) Mi-2BL1 Mi-2BL3 Mi-2BL7 Mi-2BL12 lethal | larval stage | recessive Mi-2S000606 Mi-2S085401 lethal | recessive Mi-24 Mi-25 Mi-26 Mi-27 Mi-28 Mi-29 Mi-201058 Mi-2BL1 Mi-2BL3 Mi-2BL7 Mi-2BL12 Mi-2j3D4 Mi-2K1 Mi-2L1243 Mi-2S000606 Mi-2S085401 partially lethal - majority die Mi-201058 viable Mi-2EY13252 viable, with Scer\GAL4pnr-MD237 Mi-2GD4511 Sterility Allele fertile Mi-2EY13252 Other Phenotypes Allele neuroanatomy defective Mi-2j3D4 Phenotype manifest in Allele dendrite Mi-2j3D4 egg | germline clone Mi-2K1 Mi-26 Mi-24 Mi-21 Mi-27 Mi-2j3D4 Mi-25 eye photoreceptor cell | somatic clone Mi-2unspecified retina | somatic clone Mi-2unspecified
Classical Alleles ( 55 )
For All Classical Alleles Show
Allele of Mi-2 Class Mutagen Stocks Known lesion Mi-24 ethyl methanesulfonate 1 Yes Mi-25-SZ-3915 1 -- Mi-2c00082 1 -- Mi-2c00783 piggyBac transposase 1 -- Mi-2c03115 piggyBac transposase 1 -- Mi-2c06248 piggyBac transposase 1 -- Mi-2CB-0278-3 1 -- Mi-2CB-5544-3 1 -- Mi-2CB-5569-3 1 -- Mi-2CB-6396-3 1 -- Mi-2EY08138 P-element activity 1 Yes Mi-2EY08696 P-element activity 1 Yes Mi-2EY13252 P-element activity 1 Yes Mi-2f08103 piggyBac transposase 1 -- Mi-2j3D4 P-element activity 1 Yes Mi-2KG06222 1 -- Mi-2L1243 P-element activity 1 -- Mi-2NP0394 1 -- Mi-2NP0896 1 -- Mi-2NP2346 1 -- Mi-2NP3126 1 -- Mi-2NP3581 1 -- Mi-2NP4761 1 -- Mi-2NP7524 1 -- Mi-201058 P-element activity 0 Yes Mi-21 P-element activity 0 -- Mi-25-HA-2824 0 -- Mi-25-SZ-4114 0 -- Mi-25 ethyl methanesulfonate 0 Yes Mi-26 ethyl methanesulfonate 0 Yes Mi-27 ethyl methanesulfonate 0 -- Mi-28 ethyl methanesulfonate 0 -- Mi-29 ethyl methanesulfonate 0 -- Mi-2Act5C.T:Zzzz\FLAG 0 Yes Mi-2BL12 ethyl methanesulfonate 0 Yes Mi-2BL1 ethyl methanesulfonate 0 Yes Mi-2BL3 ethyl methanesulfonate 0 Yes Mi-2BL7 ethyl methanesulfonate 0 Yes Mi-2c05371 piggyBac transposase 0 -- Mi-2c06936 piggyBac transposase 0 -- Mi-2CA06598 P-element activity 0 Yes Mi-2CA06613 P-element activity 0 -- Mi-2CPTI000020 piggyBac transposase 0 Yes Mi-2CPTI000106 piggyBac transposase 0 Yes Mi-2CPTI001119 piggyBac transposase 0 Yes Mi-2DG12505 P-element activity 0 -- Mi-2DG14402 P-element activity 0 -- Mi-2EP1113 P-element activity 0 Yes Mi-2K1 0 Yes Mi-2S000606 Delta2-3 P-element activity 0 Yes Mi-2S005504 0 -- Mi-2S047526 0 -- Mi-2S085401 Delta2-3 P-element activity 0 Yes Mi-2S147412 0 -- Mi-2unspecified 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mi-2 Class Mutagen Stocks Known lesion Mi-2GD4511 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mi-2GL00318 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mi-2HMS00301 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mi-2KK100517 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mi-2arm.PK in vitro construct - regulatory fusion 0 Yes Mi-2ΔC.Act5C.T:Zzzz\FLAG in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)25-21 (Cooper et al., 2010) Df(3L)ash1A16 (Cooper et al., 2010) Df(3L)BSC1 (Bloomington Drosophila Stock Center, 1999.2.23) Df(3L)BSC2 (Bloomington Drosophila Stock Center, 1999.2.23, Cooper et al., 2010) Df(3L)BSC445 (Cooper et al., 2010) Df(3L)ED4858 (Cooper et al., 2010) Df(3L)Exel9007 (Cooper et al., 2010) Df(3L)Exel9008 (Cooper et al., 2010) Df(3L)Exel9009 (Cooper et al., 2010) Df(3L)kto2 (Kennison, 1995.6.21, Spradling et al., 1999, Cooper et al., 2010) Df(3L)XS411 (Cooper et al., 2010) Df(3L)XS533 (Cooper et al., 2010) Df(3L)XS543 (Cooper et al., 2010) Df(3L)XS572 (Cooper et al., 2010) Df(3L)XS917 (Cooper et al., 2010) Df(3L)XS2182 (Cooper et al., 2010) Df(3L)Δ40 (Khattak et al., 2002)
Not disrupted in	Df(3L)A23 (Cooper et al., 2010) Df(3L)BSC20 (Cooper et al., 2010) Df(3L)BSC417 (Cooper et al., 2010) Df(3L)ED228 (Cooper et al., 2010) Df(3L)ED4799 (Cooper et al., 2010) Df(3L)Exel6135 (Cooper et al., 2010) Df(3L)Exel9011 (Cooper et al., 2010) Df(3L)Exel9045 (Cooper et al., 2010) Df(3L)Exel9046 (Cooper et al., 2010) Df(3L)kto22 (Cooper et al., 2010) Df(3L)pipeA13 (Cooper et al., 2010) Df(3L)XS705 (Cooper et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4511} NA P{KK100517} NA P{TRiP.GL00318} NA P{TRiP.HMS00301} NA characterization construct P{arm-Pha.K} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Mi-2j3D4 No P{lacW}Mi-2L1243 No P{lacW}Mi-2S000606 No P{lacW}Mi-2S005504 No P{lacW}Mi-2S047526 No P{lacW}Mi-2S085401 No P{lacW}Mi-2S147412 No P{lArB}Mi-21 No P{PZ}Mi-201058 No insertion of mobile activating element P{EP}EP1113EP1113 NA P{EPgy2}Mi-2EY08696 NA P{EPgy2}Mi-2EY13252 NA P{EPgy2}Su(Tpl)EY08138 NA P{GSV1}C752C752 NA P{XP}CR32218d01680 NA P{XP}CR32218d06327 NA miscellaneous insertions P{PTT-GA}Mi-2CA06598 NA P{PTT-GA}Su(Tpl)CA06613 NA P{RS3}Mi-2CB-0278-3 NA P{RS3}Mi-2CB-5544-3 NA P{RS3}Mi-2CB-5569-3 NA P{RS3}Mi-2CB-6396-3 NA P{RS5}Mi-25-HA-2824 NA P{RS5}Mi-25-SZ-3915 NA P{RS5}Mi-25-SZ-4114 NA P{SUPor-P}Mi-2KG06222 NA P{wHy}Mi-2DG14402 NA P{wHy}Su(Tpl)DG12505 NA PBac{602.P.SVS-1}Mi-2CPTI000020 NA PBac{602.P.SVS-1}Mi-2CPTI000106 NA PBac{681.P.FSVS-1}Mi-2CPTI001119 NA PBac{PB}Mi-2c00082 NA PBac{PB}Mi-2c00783 NA PBac{PB}Mi-2c03115 NA PBac{PB}Mi-2c05371 NA PBac{PB}Mi-2c06248 NA PBac{PB}Mi-2c06936 NA PBac{WH}CR32218f00697 NA PBac{WH}CR32218f07225 NA PBac{WH}Mi-2f08103 NA insertion of enhancer trap binary system P{GawB}Mi-2NP0394 No P{GawB}Mi-2NP0896 No P{GawB}Mi-2NP2346 No P{GawB}Mi-2NP3126 No P{GawB}Mi-2NP3581 No P{GawB}Mi-2NP4761 No P{GawB}Mi-2NP7524 No
Gene Ontology: Function, Process & Cellular Component ( 21 unique terms )
Terms Based on Experimental Evidence ( 13 terms )
Molecular Function
CV term Evidence References
ATPase activity inferred from direct assay (Bouazoune and Brehm, 2005, Bouazoune et al., 2002) chromatin binding inferred from direct assay (Kunert et al., 2009) DNA binding inferred from direct assay (Bouazoune et al., 2002) nucleosome binding inferred from direct assay (Bouazoune and Brehm, 2005) contributes_to nucleosome-dependent ATPase activity inferred from direct assay (Kunert et al., 2009) protein binding inferred from physical interaction with MEP-1 (Kunert et al., 2009) inferred from physical interaction with simj (Kon et al., 2005)
Biological Process
CV term Evidence References
dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) muscle organ development inferred from mutant phenotype (Parrish et al., 2006) negative regulation of transcription from RNA polymerase II promoter inferred from direct assay (Kunert et al., 2009) nucleosome mobilization inferred from direct assay (Bouazoune and Brehm, 2005, Bouazoune et al., 2002) regulation of transcription from RNA polymerase II promoter inferred from mutant phenotype (Li et al., 2010)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Kunert et al., 2009) polytene chromosome inferred from direct assay (Murawsky et al., 2001, Reddy et al., 2010)
Terms Based on Predictions or Assertions ( 11 terms )
Molecular Function
CV term Evidence References
ATPase activity non-traceable author statement (Kim et al., 1997) ATP binding inferred from electronic annotation with InterPro:IPR000330, InterPro:IPR001650, InterPro:IPR002464, InterPro:IPR012957, InterPro:IPR012958 (FlyBase Curators et al., 2004-) ATP-dependent DNA helicase activity inferred from sequence or structural similarity (Spradling et al., 1999) ATP-dependent helicase activity inferred from electronic annotation with InterPro:IPR002464 (FlyBase Curators et al., 2004-) chromatin binding inferred from sequence or structural similarity with UniProtKB:P40201 (assigned by UniProtKB) (Swiss-Prot Project Members, 2000.10.1) DNA helicase activity inferred from sequence or structural similarity with EMBL:AF425231 (Mi et al., 2001.12.13) helicase activity inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with UniProtKB:P40201 (assigned by UniProtKB) (Swiss-Prot Project Members, 2000.10.1) zinc ion binding inferred from electronic annotation with InterPro:IPR001841, InterPro:IPR001965, InterPro:IPR012957, InterPro:IPR012958 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
chromatin assembly or disassembly inferred from sequence or structural similarity with UniProtKB:P40201 (assigned by UniProtKB) (Swiss-Prot Project Members, 2000.10.1)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with UniProtKB:P40201 AND non-traceable author statement (assigned by UniProtKB) (Swiss-Prot Project Members, 2000.10.1) non-traceable author statement (Kim et al., 1997) NuRD complex inferred from sequence or structural similarity (Ahringer, 2000) traceable author statement (Murawsky et al., 2001)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Mi-2 - Ate1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - AttB anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG10496 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG10934 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG12237 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG12576 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG12818 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG14615 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG15096 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG2691 experimental knowledge based (Guruharsha et al., 2011) Mi-2 - CG31111 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG3838 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG4415 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG4813 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG5190 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG5660 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG6123 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG6227 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG6652 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG7137 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG7686 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG8735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CG8939 experimental knowledge based (Guruharsha et al., 2011) Mi-2 - CG9062 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CanB2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - CkIIβ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Crk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Dcp-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - E(spl)mδ-HLH anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Eap anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - GalNAc-T1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Hakai anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Hcs anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Hph anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - MBD-like ion exchange chromatography, molecular sieving, western blot (Ballestar et al., 2001) Mi-2 - MTA1-like ion exchange chromatography, molecular sieving, western blot (Ballestar et al., 2001) Mi-2 - Moca-cyp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - ND75 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Pitslre anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Pp2B-14D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Sbp2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Srp72 experimental knowledge based (Guruharsha et al., 2011) Mi-2 - Stat92E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - TfIIEα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Timp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - Vps4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - ana2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - ap anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - atms anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - cag anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - eIF-2α anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - fzy anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - grau anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - ihog anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - kay anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - l(1)G0020 experimental knowledge based (Guruharsha et al., 2011) Mi-2 - lectin-46Ca anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - mTerf5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - pigeon anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - stas anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mi-2 - ttk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mi-2 allele Gene References Mi-21 E(z) (Bajusz et al., 2001) Kr (Carrera et al., 1998, Abrell et al., 2000) Pc (Kehle et al., 1998) Mi-24 MBD-like (Marhold et al., 2004) Pc (Kehle et al., 1998) ttk (Murawsky et al., 2001) Mi-25 Pc (Kehle et al., 1998) Mi-26 Pc (Kehle et al., 1998) wg (Kon et al., 2005) unspecified Pc (Kehle et al., 1998) Ras85D (Read et al., 2005) Ret (Read et al., 2005)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262519
Orthologs
OrthoDB Orthologs (52) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG61G3PG Dipteran inclusive ortholog search EOG6X6CH8 Insect inclusive ortholog search EOG6F7MDS Arthropod inclusive ortholog search EOG6C5B07 Metazoa inclusive ortholog search EOG6BW4MB
Orthologs in Drosophila Species (EOG61G3PG)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Mi-2     Drosophila sechellia   Dsec\GM15785 Y   Drosophila erecta   Dere\GG13364     Drosophila yakuba   Dyak\GE22457     Drosophila ananassae   Dana\GF10842 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA23929 Y   Drosophila persimilis   Dper\GL22908     Drosophila willistoni   Dwil\GK17504     Drosophila virilis   Dvir\GJ13998 Y   Drosophila mojavensis   Dmoj\GI13663 Y   Drosophila grimshawi   Dgri\GH16907 Y
Orthologs in non-Drosophila Dipterans (EOG6X6CH8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013136   Anopheles gambiae Malaria mosquito  Agam\AGAP012009   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004665
Orthologs in non-Dipteran Insects (EOG6F7MDS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB41803   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004970   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12801   Atta cephalotes Leafcutter ant  Acep\ACEP22065   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16778   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15398   Linepithema humile Argentine ant  Lhum\LH20211   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16333   Solenopsis invicta Red fire ant  Sinv\SINV11745   Solenopsis invicta Red fire ant  Sinv\SINV11752   Acyrthosiphon pisum Pea aphid  Apim\ACYPI56610   Bombyx mori Silkmoth  Bmor\BGIBMGA002931   Pediculus humanus Human body louse  Phum\PHUM578860   Tribolium castaneum Red flour beetle  Tcas\TC012115
Orthologs in non-Insect Arthropods (EOG6C5B07)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_232072   Ixodes scapularis Deer tick  Isca\ISCW005933
Orthologs in non-Arthropod Metazoa (EOG6BW4MB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\chd-3 Y Caenorhabditis elegans Nematode  Cele\let-418 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023296gn Y Danio rerio Zebrafish  Drer\si:ch211-51m24.3 Y Danio rerio Zebrafish  Drer\si:ch211-57k11.10 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000018961 Y Xenopus tropicalis Western clawed frog  Xtro\chd3 Y Xenopus tropicalis Western clawed frog  Xtro\chd5 Y Xenopus tropicalis Western clawed frog  Xtro\chd4 Y Gallus gallus Domestic chicken  Ggal\CHD5 Y Gallus gallus Domestic chicken  Ggal\CHD4 Y Mus musculus House mouse  Mmus\Chd4 Y Mus musculus House mouse  Mmus\Chd3 Y Mus musculus House mouse  Mmus\Chd5 Y Mus musculus House mouse  Mmus\Lce1g Y Mus musculus House mouse  Mmus\Lce1d Y Rattus norvegicus Norway rat  Rnor\Chd5 Y Rattus norvegicus Norway rat  Rnor\Chd3 Y Rattus norvegicus Norway rat  Rnor\Chd4 Y Homo sapiens Human  Hsap\CHD3 Y Homo sapiens Human  Hsap\CHD5 Y Homo sapiens Human  Hsap\ENSG00000111642 Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\CHD3     Hsap\CHD5     Hsap\ENSG00000111642
AAA Orthologs (6) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM15785 Drosophila ananassae Dana\GF10842 Drosophila pseudoobscura pseudoobscura Dpse\GA23929 Drosophila virilis Dvir\GJ13998 Drosophila mojavensis Dmoj\GI13663 Drosophila grimshawi Dgri\GH16907
Stocks & Reagents
Stocks Listed in FlyBase ( 28 )
Harvard	- PBac{PB}Mi-2c00082 - PBac{PB}Mi-2c00783 - PBac{PB}Mi-2c03115 - PBac{PB}Mi-2c06248 - PBac{WH}Mi-2f08103
Kyoto	113353 w*; P{GawB}Mi-2NP3581 123105 w1118; P{RS3}Mi-2CB-0278-3 123682 w1118; P{RS3}Mi-2CB-5544-3 123690 w1118; P{RS3}Mi-2CB-5569-3 124090 w1118; P{RS3}Mi-2CB-6396-3
VDRC	v107204 P{KK100517}VIE-260B v10766 w1118; P{GD4511}v10766
	More stocks available...
Genomic Clones ( 2 )
	BACR01B21 BACR13N10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 98 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH21519 LD32039 RE55932
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT29044 GH05123 GH12789 GM02843 GM09173 GM14358 GM16112 GM21309 HL01532 LD03642 LD04612 LD09359 LD09986 LD10357 LD11390 LD11478 LD14181 LD15901 LD24613 LD26723 LD31444 LD34606 LD37894 LD42566 LD42757 LD43884 LD44726 LD46559 RE59080 RE66904 RH24665 RH36520 SD01948 SD08062 SD09321
Other clones	34974 38791 41776 43417 53117 65331 67177 80274 80313 84143 100429 105298 107249 148343 153037 214479.49 214509.40 214533.46 214565.42 275029 527072 CK01.14B.A4 CK01.25A.F5 CK01.59D.E12 CK01.106C.B4 CK01.169B.H6 Dmel_dig_014367_0623_0358 EC052220 EK044309 EK048450 EK089038 EK103863 EK145146 EK152130 EK154650 EK162609 EK166547 EK170055 EK173158 EK195950 EK212612 EK213522 EK217939 EK223125 EK243956 EK244432 EK253820 EK295905 EK302746 EN07227 EP01745 EP03543 EP08210 EP18735 FGA008H12 FGM214B06 LD30320 LD31210 Parral_28_C04 UUGC0622
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0262519 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40170
Antibody Information
	polyclonal (Murawska et al., 2008, Reddy et al., 2010, Mathieu et al., 2012)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Mi-2 l(3)L1243 (Cooper et al., 2010)
Additional comments
Other Comments
	DNA-protein interactions: genome-wide binding profile assayed for Mi-2 protein in S2 cells; GEO accession number GSE32404. (Moshkin et al., 2012) dsRNA has been made from templates generated with primers directed against this gene. RNAi of Mi-2 results in reduced arborization of ddaD and ddaE neurons, defects in muscle and defects in dendrite morphogenesis. (Parrish et al., 2006) ATPase activity, nucleosome binding and nucleosome mobilization activities are all modulated by phosphorylation of Mi-2. (Bouazoune and Brehm, 2005) The Mi-2 chromodomains are DNA binding modules. (Bouazoune et al., 2002) The Mi-2 chromodomains are important for ATP-dependent nucleosome mobilisation. (Bouazoune et al., 2002) Su(Tpl) is located in the largest intron of Mi-2, on the same strand. Overall orientation not stated: Mi-2+ Su(Tpl)+ Mi-2+ (Khattak et al., 2002) It is proposed that Mi-2 defines a novel family of chromatin remodelling complex that is mechanistically distinct from both Iswi and SWI/SNF. (Brehm et al., 2000) GenBank AF007780; AAB87384 and SPTREMBL:016102 are chimeric between Chd3 and Mi-2, which are distinct genes. (Kennison and Mueller, 2000.4.25) Isolated as a protein that interacts with hb protein in a yeast two-hybrid assay. (Kehle et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140897 NM_001014591 NM_001170005 NM_001275149 RefSeq (Proteins) • NP_649154 NP_001014591 NP_001163476 NP_001262078 DDBJ / EMBL / GenBank • AA202797 AA695449 AA951524 AAD17276 AAF49099 AAF49099.2 AAM29373 AAX52739 ACZ94747 AF119716 AGB94771 AQ026242 AQ026314 AY113368 CL528083 CL528124 CZ466713 CZ468437 CZ469920 CZ470539 CZ471099 CZ490803 Entrez Gene - A searchable database of RefSeq genes. • 40170 UniProtKB/Swiss-Prot • O97159 UniProtKB/TrEMBL • E1JI46 Q59E34
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • P-loop containing nucleoside triphosphate hydrolase (IPR027417) SNF2-related (IPR000330) Chromo domain/shadow (IPR000953) Helicase, C-terminal (IPR001650) Zinc finger, PHD-type (IPR001965) DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (IPR002464) Domain of unknown function DUF1086 (IPR009462) Domain of unknown function DUF1087 (IPR009463) Zinc finger, FYVE/PHD-type (IPR011011) CHD, C-terminal 2 (IPR012957) CHD, N-terminal (IPR012958) Zinc finger, RING-type (IPR001841) Helicase, superfamily 1/2, ATP-binding domain (IPR014001) Chromo domain-like (IPR016197) Zinc finger, PHD-finger (IPR019787) Zinc finger, PHD-type, conserved site (IPR019786) Zinc finger, RING/FYVE/PHD-type (IPR013083) Chromo domain (IPR023780)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262519 DRSC - Results from RNAi screens. • FBgn0262519 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0262519 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40170 modMine - Data generated by the modENCODE project. • FBgn0262519
Synonyms & Secondary IDs ( 26 )
Reported As
Symbol Synonym	0006/06 (Deak et al., 1997) 0854/01 (Deak et al., 1997) CG8103 (Whitfield, 2000.4.27, Bienz, 2006, Fouix et al., 2003, Quinones-Coello, 2007, Molnar et al., 2006, Stielow et al., 2008, Keleman et al., 2009.8.5, Schneiderman et al., 2009, Reddy et al., 2010, Ni et al., 2010.12.1, Ni et al., 2011.7.19) dMi (Bajusz et al., 2001) dMi-2 (Kharchenko et al., 2011, Murawska et al., 2011, Polo, 2006, Read et al., 2005, Kon et al., 2005, Levine et al., 2004, Perrin et al., 2003, Brehm et al., 2004, Lusser and Kadonaga, 2003, Neely and Workman, 2002, Clyde and Small, 2003, Khattak et al., 2002, Bouazoune et al., 2002, Murawsky et al., 2001, Eissenberg, 2001, Ballestar et al., 2001, Zbylut et al., 2001, Saurin et al., 2001, Barlow et al., 2001, Ahringer, 2000, Farkas et al., 2000, Brehm et al., 2000, Abrell et al., 2000, Mueller, 1999.12.15, Tweedie et al., 1999, Mueller, 1999.1.12, Fritsch et al., 1999, van Lohuizen, 1999, Wade and Wolffe, 1999, Aasland, 1999, Miska, 1999, Aasland, 1999, Kehle et al., 1998, Fouix et al., 2003, Murawska et al., 2008, Smith et al., 2008, Kunert et al., 2009, Hirose et al., 2002, Li et al., 2010, Nakayama et al., 2012, Fasulo et al., 2012) dMI-2 (Halbach et al., 2000, Wang and Brock, 2003, Demakov et al., 2011) dMi2 (Thompson and Travers, 2008, Sakoparnig et al., 2012) Dm-Mi-2 (Lee et al., 1999) hip76 (Kehle et al., 1998) l(3)76BDe (Cooper et al., 2010) l(3)01058   l(3)A154.3M3 (Carrera et al., 1998, ) l(3)j3D4   l(3)L1243   l(3)S000606 (Szeged Stock Center, 1998-, ) l(3)S085401 (Szeged Stock Center, 1998-, ) Mi-2 (Ji et al., 2012, Gan et al., 2010, Knowles-Barley et al., 2010, Kallappagoudar et al., 2010, Smulders-Srinivasan et al., 2010, Cooper et al., 2010, Levine and Begun, 2008, Bouazoune and Brehm, 2005, Bienz, 2006, Buszczak et al., 2007, Quinones-Coello, 2007, Molnar et al., 2006, Stielow et al., 2008, Doheny et al., 2008, Nie et al., 2009, van Vugt et al., 2009, Schneiderman et al., 2009, Wasbrough et al., 2010, Davis et al., 2011, Bajpe et al., 2008, Antao et al., 2012) Mi2 (Varga-Weisz, 2006, Mason et al., 2004, Mendjan et al., 2006, Parrish et al., 2006, Reddy et al., 2010) MI2 (Sanjuan and Marin, 2001, Schwartz and Pirrotta, 2007, Moshkin et al., 2012) Mi 2 (Lasko, 2000) MI-2 (Marhold et al., 2004) Pha (Spradling et al., 1999, Bloomington Drosophila Stock Center, 1999.2.23, BDGP Project Members, 1994-1999, ) pha (Kim et al., 1997)
Name Synonym	hb-interacting protein 76 (Kehle et al., 1998) lethal (3) L1243   Mi-2   PHD-containing ATPase (Kim et al., 1997, )
Secondary FlyBase IDs
	FBgn0010735 FBgn0011790 FBgn0013591 FBgn0024536 FBgn0028080 FBgn0028245
References ( 135 )
Generate a list of	All references relating to this gene ( 135 )
List References by type	Research paper  ( 68 ) Supplementary material  ( 8 ) Review  ( 20 ) Personal communication to FlyBase  ( 24 ) Abstract  ( 6 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 3 )
Recent research papers ( 15 )
	Lindsley et al., 2013, PLoS ONE 8(2): e55915 Anent the Genomics of Spermatogenesis in Drosophila melanogaster. [FBrf0220812] Antao et al., 2012, Mol. Cell. Biol. 32(12): 2170--2182 Protein Landscape at Drosophila melanogaster Telomere-Associated Sequence Repeats. [FBrf0218429] Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Crawford et al., 2012, Cell Death Differ. 19(12): 2040--2048 Conservation of caspase substrates across metazoans suggests hierarchical importance of signaling pathways over specific targets and cleavage site motifs in apoptosis. [FBrf0220233] Fasulo et al., 2012, PLoS Genet. 8(8): e1002878 The Drosophila MI-2 chromatin-remodeling factor regulates higher-order chromatin structure and cohesin dynamics in vivo. [FBrf0219217] Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Mathieu et al., 2012, Nucleic Acids Res. 40(11): 4879--4891 Recruitment of the ATP-dependent chromatin remodeler dMi-2 to the transcribed region of active heat shock genes. [FBrf0219222] Moshkin et al., 2012, Mol. Cell. Biol. 32(3): 675--688 Remodelers Organize Cellular Chromatin by Counteracting Intrinsic Histone-DNA Sequence Preferences in a Class-Specific Manner. [FBrf0217223] Nakayama et al., 2012, Development 139(24): 4582--4590 The PBAP remodeling complex is required for histone H3.3 replacement at chromatin boundaries and for boundary functions. [FBrf0219981] Sakoparnig et al., 2012, J. Comput. Biol. 19(2): 126--138 Binding profiles of chromatin-modifying proteins are predictive for transcriptional activity and promoter-proximal pausing. [FBrf0217402] Davis et al., 2011, BMC Dev. Biol. 11: 66 Identification of common and cell type specific LXXLL motif EcR cofactors using a bioinformatics refined candidate RNAi screen in Drosophila melanogaster cell lines. [FBrf0216830] Demakov et al., 2011, BMC Genomics 12: 566 Protein composition of interband regions in polytene and cell line chromosomes of Drosophila melanogaster. [FBrf0216974] Kharchenko et al., 2011, Nature 471(7339): 480--485 Comprehensive analysis of the chromatin landscape in Drosophila melanogaster. [FBrf0213294] Kirilly et al., 2011, Neuron 72(1): 86--100 Intrinsic epigenetic factors cooperate with the steroid hormone ecdysone to govern dendrite pruning in Drosophila. [FBrf0216319] Murawska et al., 2011, PLoS Genet. 7(7): e1002206 Stress-Induced PARP Activation Mediates Recruitment of Drosophila Mi-2 to Promote Heat Shock Gene Expression. [FBrf0214629] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...